580 results about "Methane production" patented technology

The invention provides a coal bed gas productivity prediction method and system combined with artificial intelligence, and the method comprises the steps: firstly obtaining a multi-source coal bed gas productivity data set, which comprises geological structure data, reservoir physical property data and historical production dynamic data, of a target block, and then carrying out the time-space alignment processing of the multi-source coal bed gas productivity data set, the method comprises the following steps: obtaining a standardized coal bed gas productivity characteristic set, then performing characteristic extraction on the standardized coal bed gas productivity characteristic set to generate a prediction characteristic set containing geological attribute characteristic and dynamic attribute characteristic dual codes, and then performing iterative prediction on the prediction characteristic set based on a reinforcement learning strategy network. Finally, an optimization strategy set is generated according to the target coalbed methane productivity prediction result and error distribution of historical production dynamic data and fed back to a coalbed methane production control system to trigger parameter adjustment operation, and therefore the accuracy of coalbed methane productivity prediction can be improved, and optimization control over the production process is achieved.

The invention relates to a system for producing methane through biogas reflux coupling microbial electrolysis cell (MEC) enhanced anaerobic digestion and application. A reactor system comprises an MEC anaerobic digestion system and a biogas reflux system. The MEC anaerobic digestion system is provided with a biological anode plate and a biological cathode plate, electroactive microorganisms attached to the biological anode plate convert organic matters into carbon dioxide and generate electrons, and electroactive microorganisms attached to the biological cathode plate convert carbon dioxide into methane; a secondary biogas separation tank is adopted for biogas separation in the biogas backflow system, carbon dioxide in the biogas is more effectively separated out, and the carbon dioxide intermittently flows back to a biological cathode plate of the MEC anaerobic digestion system after being collected. Compared with the prior art, the anaerobic digestion treatment of the organic solid waste is realized, and the in-situ upgrading of the biogas is also realized; and the separated carbon dioxide reflux can be efficiently methanated, so that the emission of the carbon dioxide is reduced, the in-situ treatment of the carbon dioxide is realized, the intermediate links are reduced, and the cost is reduced.

To provide a methane manufacturing system that can reduce energy loss.SOLUTION: A system comprises a steam generating device 11 that generates steam, an electrolysis methanation device 12 that introduces a first gas mainly composed of carbon dioxide through a first pipe P1 and also introduces the steam generated in the steam generating device 11 through a second piping P2, causing electrolysis of the steam and the Sabatier reaction to produce a second gas mainly composed of methane and oxygen-containing steam that is steam containing oxygen, and sending the second gas to a third pipe P3 and the oxygen-containing steam to a fourth pipe P4. and a return pipe P5 that supplies the oxygen-containing steam sent to the fourth pipe P4 to at least one of the steam generating device 11 and the second pipe P2.SELECTED DRAWING: Figure 1

The invention provides a multi-target collaborative optimization scheduling method for a hydrogen-containing comprehensive energy system, and the method comprises the steps: building a hydrogen energy diversified utilization model containing electrolytic hydrogen production, hydrogen-to-methane production, hydrogen-to-electricity conversion, carbon capture, hydrogen storage and thermoelectric hydrogen doping, and fully absorbing surplus wind power new energy; on the basis, economy and low-carbon property are taken into consideration, a hydrogen-containing comprehensive energy system multi-target optimization scheduling model with minimum total operation cost and minimum carbon emission as targets is established, the model is solved in combination with an epsilon-constraint method and a TOPSIS decision-making method, and quantity-quality cooperative operation of the comprehensive energy system is promoted. Under the condition that the hydrogen doping ratio of fuel gas is fixed, the total cost and the carbon emission amount are reduced along with the increase of the hydrogen doping ratio of the CHP unit, and when the hydrogen doping ratio reaches up to 20%, the economic benefit and the environmental benefit of the IES are the best. Compared with a traditional IES optimization scheduling problem, multi-objective optimization can better promote quantity-quality cooperative operation of the IES, the overall performance of the comprehensive energy system is improved, and the method can be widely applied and popularized.

The invention provides an underground gasification method for generating methane through reaction of hydrogen and an underground coal seam and application, and relates to the technical field of underground in-situ methane production of coal. According to the method, hydrogen is injected into the underground coal seam and reacts in the presence of a catalyst under the heating and pressurizing conditions, methane-rich gas is directly generated, the methane content is 95% mol-98.5% mol, the H2 content is 0.1% mol-3% mol, and CO2 is smaller than or equal to 0.1% mol. The underground gasification method does not involve tedious steps of oxygen oxidation, carbon dioxide reduction and the like, coal is directly converted into methane, and simple production of methane is achieved; according to the method, the traditional coal mining process is changed fundamentally, the deep underground methane direct mining is really realized, the direct coal mining is converted into the direct gasification methane production, and the technical blank of generating the methane through the in-situ reaction of the hydrogen and the underground coal seam is filled.

The invention belongs to the technical field of biomass utilization, and particularly relates to a method for preparing methane through in-situ modified charcoal reinforced anaerobic fermentation. According to the method, biochar is added into an anaerobic fermentation hydrolytic acidification system, the biochar is beneficial to enhancing interspecific electron transfer capability (DIET), hydrolytic acidification is promoted, and meanwhile, organic acids (acetic acid, butyric acid and the like) generated in the anaerobic fermentation process are utilized to perform in-situ modification on the biochar; the in-situ modified charcoal can promote anaerobic fermentation to produce methane and gas and improve the quality, so that the gas production rate and the methane production content are increased, and meanwhile, the acidification modified charcoal is simply, conveniently and efficiently realized. According to the method, the characteristic that the biochar is easy to load and modify is fully utilized, and different characteristics of different stages of an anaerobic fermentation technology are combined, so that acidification in-situ modification of the biochar is realized, and anaerobic fermentation is promoted to produce methane, produce gas and improve quality.

The invention discloses a carbon quantum dot loaded diatom electrocatalyst and a preparation method and application thereof. The preparation method comprises the following steps: dissolving and mixing diethylene triamine pentaacetic acid and salt of metal M1 in water, and performing reflux reaction to obtain a complex solution of the metal M1; adding salt of metal M2 for reflux reaction, filtering after the reaction is finished, and removing a solvent from filtrate to obtain a solid complex of bimetals M1 and M2; drying and calcining to obtain a diatomic electrocatalyst precursor; and carrying out post-treatment and freeze-drying to obtain the diatomic electrocatalyst. The carbon quantum dot loaded bimetallic atom site catalyst is obtained by a preparation strategy of coupling molecular design and quantum dot confinement, and is used for research on deep reduction of carbon dioxide into methane through electro-catalysis. The obtained catalyst has excellent methane production activity on an electro-catalysis carbon dioxide reduction reaction under an alkaline condition and a flow cell configuration, and still has relatively good stability under long-term work.

The utility model provides a kitchen garbage treatment system which comprises a garbage collection device and is characterized in that the garbage collection device is connected with the inlet end of a sorting and pulping device, the outlet end of the sorting and pulping device is connected with a sand and impurity removal device, and the outlet end of the sand and impurity removal device is connected with the inlet end of a damp and hot hydrolysis device; the outlet end of the moist heat hydrolysis device is connected with the three-phase centrifugal device, a liquid outlet and a slag outlet of the three-phase centrifugal device are connected with a feed port of the homogeneous mixing tank, a discharge port of the homogeneous mixing tank is connected with the wet anaerobic reactor, and the wet anaerobic reactor is connected with the solid-liquid separation device through a biogas slurry discharge port. A liquid phase outlet of the solid-liquid separation device is connected with a carbon source storage and compounding device through an MAP sedimentation tank; organic matters rich in the kitchen garbage are converted into a biological composite carbon source with a high additional value, and compared with a traditional kitchen garbage treatment technology in which the kitchen garbage is used for producing methane, the method has certain advantages, the income of main products is increased, and the generation of by-products and wastes is reduced.

The invention relates to the field of sludge circulation treatment, in particular to a sludge circulation treatment method capable of reducing pollution and carbon. The sludge circulation treatment method capable of reducing pollution and carbon comprises the following treatment steps: S1, sludge disintegration: selecting a sludge sample in sewage, and screening out dominant bacteria and reproduction conditions; s2, anaerobic digestion treatment: adding the screened strains into a sludge anaerobic digestion device for anaerobic digestion treatment; s3, sewage treatment: conveying the sewage subjected to anaerobic digestion treatment into a sewage treatment device, and selecting organic matters dissolved out of sludge in the step of anaerobic digestion treatment as a denitrification carbon source for biological treatment. According to the present invention, the sludge digestion innovative process is adopted, the original anaerobic reaction process in the sewage process is utilized, the decomposition and acidification effects on the biochemical excess sludge are enhanced, the methanogenesis reaction is inhibited through the internal ammonia nitrogen, the dissolution acid production efficiency is improved, the reaction time is shortened, and the on-site resource utilization of the sludge is achieved.

The invention relates to the technical field of further improvement of oil field recovery efficiency and CCUS in the later stage of CO2 oil displacement, and discloses a method for producing methane from crude oil, which comprises the step of degrading the crude oil in a crude oil produced liquid sample in the presence of a biological surface active substance to generate a first product containing methane. According to the invention, exogenous microorganisms are cooperated with indigenous microorganisms to form an oil extraction system for producing methane by using CO2 and crude oil, and biological factors are combined with non-biological factors to integrally improve the efficiency of converting CO2 into methane.

The invention discloses a method for efficiently degrading sludge containing a large amount of microplastics through sodium hydroxide hydrothermal pretreatment. The method comprises the following steps: injecting sodium hydroxide and activated sludge containing micro-plastics into a reaction kettle together, continuing for 30 minutes under the condition of 170 DEG C, taking out the reaction kettle after the reaction is finished, and naturally cooling. The obtained sludge is fed into a sludge anaerobic reactor in a daily feeding form, all the reactors operate in a water bath at 37 DEG C under the stirring condition of 120 rpm, and various pollutant indexes, methane production amount changes and the like in an anaerobic system are monitored every day. The method can effectively improve the degradation efficiency of the sludge containing a large amount of micro-plastics, improve the stability and resource utilization efficiency of the sludge, obviously reduce the content of the micro-plastics in a sludge system and reduce environmental pollution.

To provide a methane producing apparatus that can reduce an amount of methanation catalyst.SOLUTION: A methane producing apparatus 1 produces methane by allowing raw material gas to pass through a first reactor 10 and a second reactor 20. Each reactor comprises: a heating part 112 which heats raw material gas to an optimal temperature; a reaction part 113 which allows raw material gas to perform methanation reaction by a catalyst maintained at an optimal temperature; a cooling part 114 which cools reaction gas; and a heat medium flow channel 13 which is a flow channel arranged in a state of surrounding the parts, and through which heat medium is flown. A heat medium in a first temperature zone which has been supplied to the heat medium flow channel performs heat exchange with reaction gas of the cooling part 114 to enter a second temperature zone higher than the first temperature zone, performs heat exchange with a catalyst of the reaction part 113 to enter a third temperature zone higher than the second temperature zone, and performs heat exchange with raw material gas of the heating part 112 to enter a fourth temperature zone lower than the third temperature zone and higher than the first temperature zone.SELECTED DRAWING: Figure 1

The invention provides a high-crystallinity modified iron oxide as well as a preparation method and application thereof, and relates to the technical field of organic solid waste treatment. The preparation method comprises the following steps: preparing a precursor MIL-88B by utilizing a hydrothermal method; the precursor MIL-88B is subjected to calcination treatment, and an iron oxide with the MIL-88B as the precursor is obtained; the temperature condition of the calcination treatment is 550-850 DEG C. The high-crystallinity modified iron oxide prepared by the preparation method improves methane production efficiency and stability, avoids competition of iron reducing bacteria, is stable in long-term operation and low in cost, and has both environmental and economic benefits.

The invention relates to a shale or coal rock gas evaluation method based on a gas-water two-phase flow isotope fractionation model. The shale or coal rock gas evaluation method comprises the following steps: 1, collecting data; 2, establishing a gas well model; 3, establishing a control equation set; 4, coupling the influence of the real gas effect and the effective stress; 5, establishing a gas transmission model and a relative permeability model; 6, establishing a relation between capillary pressure and saturation; 7, considering critical desorption pressure; 8, setting boundary conditions of initial conditions; 9, solving a control equation set; 10, calculating production dynamic parameters and isotope values; 11, performing history fitting; 12, verifying historical fitting quality; 13, predicting productivity; 14, calculating a gas dynamic output proportion; 15, identifying a fractionation inflection point and gas production mechanism conversion; by coupling gas-water two-phase flow, multiple gas transmission mechanisms, stress sensitivity and methane isotope competitive adsorption theories, yield prediction of the deep reservoir and quantitative evaluation of the dynamic proportion of produced gas are realized.

The invention provides a kitchen garbage biomass energy resource cascade recovery system, which comprises a pretreatment unit, a biomass energy resource cascade recovery unit, a biomass energy resource cascade recovery unit and a biomass energy resource cascade recovery unit, and is characterized in that the pretreatment unit is used for pretreating kitchen garbage and straws to obtain slurry and solids; the solid phase treatment unit comprises a biological hydrolysis device, a biological fermentation device and a biological decomposition device which are connected in sequence; the biological hydrolysis device is connected with the pretreatment unit; the product utilization unit is connected with the biological decomposition device; the liquid phase treatment unit comprises a dark fermentation hydrogen production device, a microbial electrolysis cell hydrogen production device and an anaerobic digestion methane production device which are connected in sequence; the dark fermentation hydrogen production device is connected with the pretreatment unit; and the tail water utilization unit is connected with the anaerobic digestion methane production device. The gas, the liquid and the solid generated by the recovery system are subjected to effective energy recovery and maximum resource utilization, no three wastes are generated, and the recovery system can be widely applied to the fields of agriculture, environmental protection, energy and the like.

The invention discloses an anaerobic fermentation tank for livestock and poultry breeding mixed liquid dung, and belongs to the technical field of livestock and poultry waste treatment and utilization. The device is structurally provided with a steel tank body, a water inlet, an exhaust port, a water outlet, a heat exchange pipe, a slag discharge port and a pressure gauge. The water inlet, the exhaust port, the drainage port and the pressure indicator are arranged at the upper end of the tank body; the deslagging port and the heat exchange pipe are arranged at the lower end of the tank body; the interior of the tank body is divided into a plurality of independent cabins by isolation baffles, and the adjacent cabins are sequentially connected in series through cabin flow guiding openings and cabin slag discharging openings. Each cabin is divided into a plurality of small chambers by flow guide plates, and the adjacent small chambers are sequentially connected in series through small chamber exhaust holes and small chamber slag discharge holes. The heat exchange pipe is arranged at the bottom upper part of the tank body and annularly penetrates through the cabins; and the slag discharge port is arranged at the bottom of the serial tail cabin. According to the invention, the energy-consumption-free repeated settling separation of suspended matters in the liquid dung is fully realized, the solid / liquid separation efficiency of the liquid dung is improved, the methane content in unit liquid dung is high, the concentration of suspended matters in biogas slurry is low, the biogas residue is less, the problems of surface crusting, pipeline blockage and the like are avoided during long-term operation of equipment, and the operation and maintenance cost is low.

The invention relates to a sea surface methane concentration inversion prediction method and system based on a time sequence neural network, and relates to the technical field of marine environment monitoring processing, and the method comprises the steps: taking obtained water color remote sensing data and environmental parameters as references, and performing inversion analysis on optical parameters and diffusion attenuation coefficients; and further inverting a key driving quantity reflecting a main physical process of the change of methane on the sea surface, extracting time sequence characteristics with physical significance, inputting the time sequence characteristics into a time sequence neural network model group to invert a methane concentration value, and predicting a methane concentration value in a certain time period. Therefore, starting from the processes of sea surface methane production and sea surface methane concentration change, the influence of factors such as optical characteristics, environmental parameters and various driving quantities on sea surface methane production and emission is deeply studied, on one hand, the sea surface methane concentration value with the known target time is accurately inverted, and on the other hand, the sea surface methane concentration value with the known target time is accurately inverted. The methane concentration value in a future time period can be predicted, and the monitoring efficiency and applicability are improved.

The present invention provides a method for anaerobic digestion of sludge to produce methane based on direct interspecies electron transfer. A conductive material is added to the sludge, and an anaerobic digestion reaction is carried out under stirring; the conductive material is an SC / FeS composite material. In the present invention, the SC / FeS composite material is added to the sludge as the conductive material. Due to the good biocompatibility, fast mass transfer ability and electron transfer ability of SC; as well as the FeS particles with good conductivity, the anaerobic digestion efficiency of microorganisms can be improved, the resource utilization of wastewater treatment can be realized, and the methane production can be increased by 1.2 - 3.75 times.

The present invention discloses a method for improving the methane production and purity in the anaerobic fermentation of straw-like raw materials, which comprises the following steps: S1. Pretreatment; S2. Inoculating the pretreated initial liquid material with hydrogen-producing and acid-producing microorganisms, and carrying out anaerobic fermentation by means of magnetic field strengthening and external circulation; S31. Collecting the fermentation gas in step S2, preparing nano-bubble water, then adding the pretreated initial liquid material, inoculating hydrogenophilic methanogenic microorganisms, and carrying out anaerobic fermentation to produce methane; S32. Collecting the fermentation liquid in step S2, inoculating acetic acidophilic methanogenic microorganisms, and carrying out anaerobic fermentation to produce methane by means of magnetic field strengthening and external circulation. This application separately uses acid-producing substrates and hydrogen to separate the methanogenesis stage into two independent pathways of acidophilic methanogenesis and hydrogenophilic methanogenesis. Combining magnetic field strengthening and nano-bubble technology, it improves the organic matter degradation effect, hydrogen-producing and acid-producing effect, as well as the utilization efficiency of hydrogen and acid-producing substrates, increases the methane production and purity, and shortens the entire fermentation cycle.

The invention discloses a dry-type high-pressure anaerobic fermentation system and method for agricultural waste, and belongs to the technical field of biogas fermention.The dry-type high-pressure anaerobic fermentation system comprises a mixing pool, a feeding tank and an anaerobic fermentation reactor, and the feeding tank is connected with a hydrolysis pool feeding pipeline and an acid production pool feeding pipeline; the anaerobic fermentation reactor comprises a hydrolysis tank, an acid production tank and a methane production tank which are separated by a reactor outer wall, a first separation wall and a second separation wall which are arranged in a concentric circle, and a biogas collection area formed by a separation top plate and a reactor dome is arranged at the top end of the reactor outer wall; an overflow channel is formed at the top end of the first separating wall; a plurality of separating wall supporting columns are circumferentially and uniformly distributed at the bottom of the second separating wall; a leachate tank is correspondingly arranged at the bottom end of the methanogenesis tank, and a plurality of biogas slurry lifting pipes are arranged in the methanogenesis tank; a fermentation waste discharge pipeline is arranged on the side wall of the second separation wall, a biogas discharge pipe is arranged on the dome of the reactor, and the fermentation system is stable in operation, small in consumption of circulating inoculum and high in volume gas production rate.

The invention relates to a pre-fermentation method for improving biogas production of straws and a biogas production fermentation system. The pre-fermentation method comprises the following steps: S1, preparing a mixed microbial agent to be fermented; the mixed microbial agent is prepared by inoculating the following zymophyte in parts by weight into a fermentation culture medium according to the total inoculation amount of 3-10% (v / v): 5-50 parts of bacillus subtilis, 10-20 parts of white-rot fungi, 5-10 parts of aspergillus oryzae, 5-10 parts of trametes versicolor and 5-10 parts of trichoderma viride; s2, uniformly mixing the mixed microbial agent with the micro-nano bubble water, and fermenting to form an enhanced hydrolysis bacterium solution containing a microbial agent fermentation solution and an enzyme solution; s3, spraying enhanced hydrolysis bacteria liquid onto the straws for enhanced hydrolysis so as to complete pretreatment before fermentation and biogas production; wherein the volume of the reinforced hydrolytic bacteria liquid required by each kg of straw is 3-5L. Compared with the prior art, the mixed microbial agent and the micro-nano bubble water are used for carrying out cooperative treatment on the straw, so that the gas production efficiency and the methane production amount of the straw can be effectively improved.

The invention provides a method for producing methane by sludge anaerobic digestion based on direct interspecific electron transfer, which comprises the following steps: adding a conductive material into sludge, and carrying out anaerobic digestion reaction under stirring; the conductive material is an SC / FeS composite material. According to the invention, the SC / FeS composite material is added into the sludge as a conductive material, and the SC has good biocompatibility and rapid mass transfer capability and electron transfer capability; according to the present invention, the anaerobic digestion efficiency of the microorganisms can be improved by using the activated carbon and the ferrous sulfide FeS particles with good conductivity, the wastewater treatment resource utilization can be achieved, and the methane yield can be improved by 1.2-3.75 times;

The invention discloses a continuous kettle type nitromethane production process, and relates to the technical field of basic chemical raw material synthesis and flow chemical reaction process control, which comprises the following steps: quantitatively and continuously pumping a temperature-regulated sodium nitrite mixed solution into a raw material mixer in a closed-loop automatic control mode through a variable-frequency feeding pump matched with a mass flow meter; synchronously, quantitatively and continuously pumping dimethyl sulfate into the raw material mixer in a closed-loop automatic control manner by matching a feeding pump with a stop valve and a mass flow meter, and uniformly mixing the two raw materials in the raw material mixer according to the stoichiometric molar ratio of the dimethyl sulfate to the sodium nitrite, according to the tandem type segmented design, each reaction kettle can be subjected to customized temperature field control and heat transfer design specially aiming at the special thermodynamic and dynamic requirements of the reaction stage where the reaction kettle is located, the heat transfer efficiency of the whole system is greatly improved, and due to the fact that reaction heat is taken away timely and accurately, the heat transfer efficiency is greatly improved. And a by-product generation path induced by local abnormal high temperature is effectively restrained.

The invention provides a carbon-manganese-based composite material as well as a preparation method and application thereof. The carbon-manganese-based composite material is a MnO2-BC composite material formed by MnO2 and biochar, and MnO2 nanoparticles are loaded on the surface of the biochar; by adding 60mg of MnO2-BC / g.VS into a WAS anaerobic fermentation system, the anaerobic fermentation performance of the WAS can be greatly improved, and the degradation rates of polysaccharide, protein and SCOD (soluble chemical oxygen demand) are improved; the method can promote the directional enrichment of Desulfovibrio and Geobacter from the angles of energy metabolism regulation and electron directional transduction optimization, stably and efficiently solves the bottleneck problems of easy acid accumulation, poor flora synergistic effect, easy environmental stress in methane production and the like in the traditional WAS anaerobic fermentation, and has excellent application potential.

The present disclosure relates to ruminant feed additive composition comprising a combination of urea, biuret and N-containing by-products of the biuret production out of urea, and optionally one or more nitrate compounds, to inhibit methane production emanating from the activities of the digestive tract of ruminant animals, the composition comprising one or more other methane inhibition compounds, chosen out of the group consisting of halogenated methane analogues in the form of halogenated aliphatic compounds with 1 or 2 carbons (also called C1-C2 hydrocarbons) and halocarbon compounds, nitro compounds, plant secondary metabolites (PSM), seaweed, algae, lichen, and / or fungi, dietary lipid and / or fatty acids, The present disclosure further relates to the use of such a ruminant feed additive composition to inhibit methane emissions from digestive activities of ruminant animals and additionally mitigate the amount of nitrous oxide (N2O) and ammonia (NH4) in the urine of the ruminant animal. The present disclosure also relates to a method for inhibiting the production of methane emanating from the digestive activities of ruminant animals, wherein the method comprises the step of orally administering to the ruminant animal an effective amount of such a ruminant feed additive composition.

The present invention discloses a bi-phasic anaerobic digestion (AD) system [100] designed to produce biohydrogen, biohythane, and high-purity biogas with at least 90% methane content from various organic wastes, including lignocellulosic biomass. This system utilizes a single, closed, horizontal cylindrical reactor with two chambers: acidification chamber I and biomethanation chamber II. It facilitates high-pressure solid-state AD through optimal supplementation of nutrient nanoparticles and bioaugmented inoculum. The system's novel design, featuring a central duct, cones, and perforated plates, ensures efficient mixing and heat and mass transfer. The system can also generate biohythane, containing 15-25% hydrogen and 75-85% methane, by controlling the flow of gases between chambers. Additional features include micro-aeration to enhance hydrolysis, gravity settling chambers for slurry withdrawal, controlled gas passage between chambers to optimize methane production, and a programmable logic controller (PLC) for automated pH, temperature monitoring, and process control.

To provide: a methane production device and a methane production method which, in production of methane gas by bio-methanation, enable supply of the methane gas at a proper concentration required on a use place where the methane gas is used; and a power generation system utilizing the methane production device.SOLUTION: A methane production method according to the present invention, which is for performing methane production based on bio-methanation, includes: a reaction step of performing a methanation reaction with hydrogen gas and biogas being supplied; and a control step of controlling the efficiency of the methanation reaction to obtain methane gas having any methane concentration. According to the methane production method, the methane gas can be supplied at a proper concentration adapted to a use place of the methane gas. A power generation system according to the present invention utilizes the methane gas obtained from a methane production device according to the present invention. The power generation system enables the methane production device to have a high efficiency without replacing an existing gas power generator.SELECTED DRAWING: Figure 1

To provide means for fast and simple quantification of bacteria that inhibit methane production.SOLUTION: The present invention provides a primer set targeting uncultured bacteria of the Succinivibrionaceae family for detection, and a primer set targeting Prevotella lacticifex for detection.SELECTED DRAWING: None

The invention relates to a method for inhibiting rumen gas production by using a genetically modified laver filament as a ruminant feed additive and a method for inhibiting methane production by using the genetically modified laver filament as the ruminant feed additive. A porphyra yezoensis bromine peroxidase gene is cloned, an overexpression vector of the gene is constructed, porphyra yezoensis thallus is used as a receptor for genetic transformation, and a porphyra algal strain of which the key enzyme expression quantity is remarkably improved is obtained through screening. The method comprises the following steps: inducing thalli to generate protonema, crushing the protonema by using a crusher, carrying out ventilated large-scale culture in a seawater culture medium added with PES by using a column bioreactor to obtain a large number of protonema materials, collecting the protonema materials, crushing the protonema materials by using the crusher, and drying at low temperature. The obtained laver protonema powder is put into a vacuum bag for low-temperature preservation in dark light, and can be applied to preparation of ruminant feed as a dairy cow feed additive. The filamentous can accumulate halogenated compounds such as bromidogen and the like, can inhibit the activity of a key enzyme generated by methane in rumen, and further inhibits the generation of methane.

The invention discloses an electric methane preparation catalyst compounded by Ni nanoclusters and ZrO2 with lattice embedded with Ru single atoms as well as a preparation method and application of the electric methane preparation catalyst. The electric methane preparation catalyst comprises a NiNCRuSA / ZrO2 bimetallic active site catalyst compounded by the Ni nanoclusters and the ZrO2 with the lattice embedded with the Ru single atoms. The electric methane preparation catalyst comprises a NiNCRuSA / ZrO2 bimetallic active site catalyst, the NiNCRuSA / ZrO2 bimetallic active site catalyst is of a ZrO2 composite structure formed by embedding Ru monatomic into Ni nanoclusters and crystal lattices, the Ni nanoclusters and the Ru monatomic are adjacently distributed on a ZrO carrier to form a synergistic catalysis structure, rich and efficient active sites are provided for catalytic reaction, and reactant adsorption and conversion are facilitated; in the subsequent CO2 methanation reaction, the Ni element quickly dissociates H2 and provides sufficient H *, Ru replaces ZrO2 to serve as an active site for CO2 adsorption and conversion, conversion of an intermediate of CO2 can be accelerated, the reaction activity is greatly enhanced through cooperation of the Ni element and the Ru, the reaction energy barrier is reduced, and the overall catalytic efficiency is improved.