The invention discloses a preparation method of an activated carbon adsorbent for regenerating and adsorbing methyl orange, which comprises the following steps of crushing an activated carbon raw material into particles with the particle size of 1.2-3 cm, soaking in an acid solution for 6-10 hours, then fishing out, washing with distilled water to be neutral, drying, soaking in an alkaline solution for 8-15 hours, then fishing out, washing with distilled water to be neutral, and drying, adding copper sulfate and ferrous sulfate into distilled water, then adding the activated carbon raw material in the step S1 into the solution, adding graphene oxide, transferring into a high-pressure reaction kettle, and carrying out high-temperature reaction, cooling, filtering and drying, putting an activated carbon raw material into a tubular furnace, introducing mixed gas, calcining for 4-8 hours, and cooling to obtain an activated carbon precursor, adding a surfactant into distilled water, then adding an activated carbon precursor, stirring, filtering, drying, then adding the dried activated carbon into a dopamine aqueous solution, adding into a 6-15% tris-HCl buffer solution, stirring for 6-12 hours, filtering, and drying to obtain the activated carbon.