303 results about "Xylanase" patented technology

The invention discloses a multifunctional cellulolytic enzyme and application thereof in degradation of agricultural by-products, and belongs to the technical field of biology. The multifunctional cellulolytic enzyme is named RuCelI, and the amino acid sequence of the multifunctional cellulolytic enzyme is as shown in SEQ ID NO. 1. Based on a rumen microbial metagenome resource mining technology, a novel multifunctional cellulose decomposition enzyme RuCelI is developed, and the enzyme has double activities of endo-cellulase and xylanase, can be used for degrading agricultural by-products, reducing the treatment cost and improving the degradation efficiency, and is beneficial to improving the utilization rate and the economic benefit of the agricultural by-products.

The invention provides Aspergillus tubingensis HNZY02, a method for producing enzymes by the Aspergillus tubingensis HNZY02 and application of the Aspergillus tubingensis HNZY02, and belongs to the technical field of microorganisms, the Aspergillus tubingensis HNZY02 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.42148. The aspergillus tubingensis HNZY02 shows excellent cellulase and xylanase synthesis capability, can synergistically and efficiently degrade main structural components such as cellulose, hemicellulose and lignin in tobacco stems, and improves the safety and application value of cigarettes while breaking the compact structure and increasing the reducing sugar content.

The invention discloses streptomyces pseudogriseogriseus for promoting straw degradation and increasing soil organic matters, and belongs to the technical field of microorganisms. The streptomyces pseudogriseolus NF3 disclosed by the invention has the activity of CMC-Na enzyme, xylanase, laccase, lignin peroxidase and manganese peroxidase, and can be used for degrading cellulose, hemicellulose and lignin at the same time, and the degradation rate of the streptomyces pseudogriseolus NF3 on corn straws reaches 67.75%; the fertilizer is applied to straw returning soil, soil nutrient release is accelerated, the soil organic matter content is increased, and the crop yield is increased.

A thermotolerant xylanase enzyme that is a variant of SEQ ID NO:2, wherein the variant comprises at least one amino acid substitution at a position selected from the group consisting of 90, 105, 114, and 115 and / or comprises any of the following sets of substitutions: (a) G48C and T206C; (b) Y123V, S125C and N171C; (c) S114C, Y123V, S125C and N171C; (d) Y123V, S125C, N171C, G48C and T206C; (e) S114C, Y123V, S125C, N171C, G48C and T206C; wherein the substitution or substitutions increases the thermotolerance of the xylanase relative to a parent xylanase lacking the substitution(s).

The application discloses a beta-1,4-xylanase mutant and a construction method thereof, and comprises the following steps: constructing a beta-1,4-xylanase recombinant expression vector; introducing the recombinant vector pNZ8048-P5-XynA into a competent lactococcus lactis by using an electrotransformation technology to construct a wild-type expression vector; on the basis, designing and constructing a multi-site mutant recombinant plasmid by using a site-directed mutation method, wherein the eighth asparagine (Asn) of the beta-1,4-xylanase is mutated into tyrosine (Tyr), the 22nd serine (Ser) is mutated into proline (Pro), and the 59th alanine (Ala) is mutated into lysine (Lys); introducing the multi-site mutant recombinant plasmid into the competent lactococcus lactis to construct a heterologous expression strain of the mutant beta-1,4-xylanase (Mut-1); and stably obtaining the mutant xylanase beta-1,4-xylanase (Mut-1) with high activity by using a metal affinity chromatography (Ni 2+ ) method. The specific enzyme activity of the mutant reaches 1929.30±5.36 U / mg, and the optimal temperature is increased by 15 DEG C compared with that of the wild type.

The invention discloses xylanase XynA00838, a truncated body of the xylanase XynA00838 for removing CBM1 and application of the xylanase XynA00838, and belongs to the technical field of microorganisms. The invention provides the XynA00838, the amino acid sequence of the truncation of the XynA00838 and the nucleotide sequence of the coding gene of the XynA00838, and provides characterization information, the XynA00838 and the truncation of the XynA00838 have obvious xylanase activity, and the specific enzyme activity of the truncation of the XynA00838 is obviously higher than that of wild type xylanase XynA00838 when six different plant polysaccharides are degraded, so that the XynA00838 and the truncation of the XynA00838 can be used for degrading various plant polysaccharides. When the truncated body degrades low-viscosity wheat arabinoxylan as a substrate, the specific enzyme activity reaches 4.46 + / -0.12 U / mg and is remarkably higher than that of wild type xylanase XynA00838. The CBM1 in the xylanase XynA00838 disclosed by the invention has a relatively good maintaining effect on the temperature stability of an enzyme catalysis module of the xylanase XynA00838. The truncated body XynA00838 delta CBM1 of the xylanase XynA00838 without the CBM1 has high enzyme activity and pH stability, so that the truncated body XynA00838 delta CBM1 has relatively great development and application potential, and a basic theoretical basis is provided for preparing a commercial feed enzyme preparation.

This invention discloses a method for preparing a carbon source for microbial culture medium based on enzymatic hydrolysis of bamboo powder and its application, belonging to the field of high-value utilization technology of biomass resources. Using bamboo powder as raw material, the invention involves mild alkali pretreatment to disrupt the dense structure of lignocellulose, followed by enzymatic hydrolysis using a specialized enzyme system of endoglucanase Cel7B and xylanase XynA expressed heterologously by *E. coli* in an optimized ratio. After solid-liquid separation, an enzymatic hydrolysate rich in reducing sugars is obtained. The hydrolysate is then nutritionally fortified with trace elements such as sodium thiosulfate and sodium silicate to produce a low-cost, high-efficiency liquid carbon source. This invention achieves high-value utilization of bamboo waste. The entire process is mild, environmentally friendly, and simple, easily scaled up industrially, and solves the industry bottleneck of high carbon source cost in industrial microbial culture, demonstrating good application prospects and market value.

The application discloses a method for preparing a hard carbon negative material for a sodium ion battery by treating biomass by using a xylanase. The method uses biomass as a precursor, removes part of hemicellulose by using a xylanase, and then carbonizes to obtain a biomass-based hard carbon for a negative electrode of a sodium ion battery. The preparation method is simple, environment-friendly, non-toxic and harmless, and conforms to the green and sustainable development concept. The prepared hard carbon negative material exhibits excellent performance in the sodium ion battery.

A variant polypeptide of xylanase, and a fusion protein and an enzyme composition comprising the variant polypeptide, a recombinant host cell for production of the variant polypeptide, methods for using it and its use, is disclosed comprising an amino acid sequence having at least 79%, but less than 100% amino acid sequence identity with amino acids 1-191 of SEQ ID NO: 1, wherein the amino acid sequence has xylanase activity, at least one disulfide bridge between two Cys residues in the 1-191 amino acid region, and an amino acid substitution at the position 23 or 28, or at the positions 23 and 28, the positions corresponding to the positions 23 and 28 of the SEQ ID NO: 1.

This invention relates to the field of fertilizer technology, specifically to an organic liquid fertilizer based on straw enzymatic hydrolysate and its preparation method. The method includes: pretreating corn straw with potassium hydroxide, followed by enzymatic hydrolysis with cellulase and xylanase to obtain the enzymatic supernatant; sequentially treating the supernatant with an ultrafiltration membrane with a molecular weight cutoff of 3000 Da and a nanofiltration membrane with a molecular weight cutoff of 500 Da to collect oligosaccharides with a molecular weight window of 500-3000 Da; partially stabilizing the reducing end of the oligosaccharides with sodium borohydride; pre-complexing with boric acid at pH 8.4-8.8; then sequentially adding relatively low molecular weight sodium polyglutamate, calcium chloride dihydrate aqueous solution, and ultra-high molecular weight sodium polyglutamate to construct a sequential complexation system; subsequently adding potassium nitrate and citric acid aqueous solution; and finally, after filtration at 5 μm and heat treatment at 70℃, obtaining the organic liquid fertilizer. This fertilizer has a high proportion of 500-3000 Da oligosaccharides and a low reducing sugar equivalent, inhibiting storage precipitation and browning, and improving the retention rate of extractable oligosaccharides in the soil.

The present application belongs to the technical field of pulping and papermaking, and provides a method for preparing a cotton-stalk biochemomechanical pulp. The present application provides a method for preparing a cotton-stalk biochemomechanical pulp, the method comprising the following steps: cutting cleaned and dried cotton stalks, and then sequentially performing pre-steaming and a screw extrusion fiberization treatment; immersing the defibrated cotton stalks into a buffer solution of cellulase to perform an enzymatic treatment for a set period of time, and then immersing same into a hot alkali solution; sequentially subjecting the cotton stalks, which have been subjected to a hot alkali impregnation treatment, to high-consistency pulp refining and a latency removal treatment, and then subjecting the resulting coarse pulp to a second-stage enzymatic treatment by using a compound enzyme of xylanase and pectinase; and subjecting the pulp, which has been subjected to the second-stage enzymatic treatment, to pulp screening and PFI beating, so as to obtain a cotton-stalk biochemomechanical pulp.

The invention discloses a method for improving the quality of ganoderma lucidum mushroom residues through synergistic fermentation of bacteria and enzymes and application of the method. According to the method disclosed by the invention, cellulase, beta-galactosidase and xylanase with proper concentrations and lactobacillus plantarum, bacillus subtilis and saccharomyces cerevisiae with proper proportions are adopted, and enzymes and bacteria are mutually cooperated, so that the characteristics of the raw materials are well balanced, the finally prepared ganoderma lucidum mushroom bran is high in protein content and low in fiber content, and the nutritional value of a feed is improved; the total amount of amino acids and essential amino acids are remarkably increased, and the feed protein quality can be improved; the rumen dry matter digestibility and energy metabolites are remarkably improved, and ruminant absorption and utilization are facilitated; the ganoderma lucidum mushroom bran fermented feed is added into the daily ration, so that the daily gain, the carcass rate and the dressing percentage of fed animals can be remarkably improved, the levels of serum GLU, TG, ALT and AST are reduced, the expression of key inflammatory cytokines (IL-1beta and IL-6) is inhibited, and the ganoderma lucidum mushroom bran fermented feed has the comprehensive effects of regulating glycolipid metabolism and relieving liver load and systemic inflammation, so that the immune function of animal bodies is improved.

The application discloses a preparation method of bio-modified wheat bran containing xylo-oligosaccharide, and comprises the following steps: fermenting wheat bran by using mixed strains and composite enzymes; wherein the mixed strains comprise lactic acid bacteria, Saccharomyces cerevisiae and Bacillus licheniformis; and the composite enzymes comprise cellulase and xylanase. The preparation method provided by the application effectively reduces the contents of crude fiber and neutral detergent fiber in the wheat bran, increases the contents of crude protein and xylo-oligosaccharide in the fermented wheat bran, which are beneficial to the growth and development of animals, and improves the nutritional value of the wheat bran, so that the proportion of the wheat bran in feed application can be increased, and the addition amount of corn and soybean meal can be reduced.

The application discloses a process for improving the quality of palm kernel meal feed by enzyme and bacteria synergistic fermentation, and belongs to the field of feed biotechnology.The process is characterized by the following steps: crushing and screening palm kernel meal, mixing the palm kernel meal with 20% bran and 4% ammonium sulfate as dry matter to obtain a basic substrate;compounding bacillus subtilis, aspergillus niger and saccharomyces cerevisiae at a ratio of 1:1:1 of viable bacterial number, adding 0.67% mannanase and 0.3% xylanase to prepare a bacteria-enzyme mixed solution, inoculating the bacteria-enzyme mixed solution at a 10% inoculation amount, adjusting the moisture content to 50% and the pH to 6.4, and then sealing and storing the bacteria-enzyme mixed solution for use after 4 days of fermentation at 33.5 DEG C; and the bacteria-enzyme mixed solution can be stored for a long time.The process can efficiently degrade crude fiber and anti-nutritional factors, improve the protein content and the digestibility, is stable and easy to mass-produce, can replace part of corn and soybean meal in the daily ration of ruminants, and realizes high-value utilization of palm kernel meal.

The invention belongs to the technical field of catalysts, and particularly relates to a carbon-coated metal oxide catalyst as well as a preparation method and application thereof. The method comprises the following steps: mixing biomass and enzyme-containing liquid, and carrying out enzymolysis treatment to obtain biomass subjected to enzymolysis treatment; enzymes in the enzyme-containing liquid comprise cellulase and xylanase; mixing the biomass subjected to enzymolysis treatment, metal salt and a nitrogen source, and sequentially performing pyrolysis in protective gas and annealing treatment in an air atmosphere to obtain a carbon-coated metal oxide catalyst; the pyrolysis comprises low-temperature pyrolysis and high-temperature pyrolysis in sequence; the temperature of the low-temperature pyrolysis is 350 to 450 DEG C; the temperature of the high-temperature pyrolysis is 700 to 800 DEG C. The carbon-coated metal oxide catalyst prepared by the invention has a multi-stage structure with a core metal oxide, a middle carbon branch channel and an outer layer N-doped charcoal, is large in specific surface area, has excellent structural uniformity and reactivity, and can improve the efficiency of biomass pyrolysis for hydrogen production.

Disclosed is a method for the production of a stable and homogeneous food composition, especially fruit preparation, comprising the steps of addition of active xylanase to plant fibre to perform an enzymation step, followed by addition of fibre solution to other food ingredients.

The invention belongs to the technical field of xylooligosaccharide preparation, and discloses a method for co-producing dissolving pulp and xylooligosaccharide by using bagasse. Comprising the following steps: (1) mixing a hydrogen bond acceptor, an alkaline hydrogen bond donor and a protective agent, and heating and stirring to prepare an alkaline eutectic solvent; (2) adding the bagasse into an alkaline eutectic solvent, and heating and stirring for reaction to obtain a reaction mixture; (3) carrying out solid-liquid separation on the reaction mixture, washing a solid component to be neutral to obtain holocellulose, and collecting a washing solution; mixing the washing solution and the liquid component, adding into absolute ethyl alcohol, standing and precipitating, then carrying out solid-liquid separation, and washing and freeze-drying the solid precipitate to obtain hemicellulose; (4) respectively performing enzymolysis on holocellulose and hemicellulose by adopting xylanase, collecting enzymatic hydrolysate and enzymolysis residues, merging the enzymatic hydrolysate, and treating to obtain xylooligosaccharide; washing and freeze-drying holocellulose enzymolysis residues to obtain the dissolving pulp. The alkaline eutectic solvent is used for assisting enzymolysis to obtain the dissolving pulp and the xylooligosaccharide.

The invention relates to a method for producing a cellulose sheet having a xylan content of less than 25.0 percent by weight and having a tear index of at least 4.00 mN∙m2∙g-1, characterised by the combination of measures that straw, which contains lignin, xylan and cellulose and is suspended in an aqueous phase, is treated by means of an alkaline aqueous solution containing ethanol in order to reduce the lignin content, as a result of which a first cellulose is obtained, the first cellulose is treated with a xylanase in order to reduce the xylan content, as a result of which a second cellulose is obtained, and a cellulose sheet is formed from the second cellulose.

The application relates to the technical field of biomaterial modification, and particularly relates to a method for carrying out directional enzymolysis treatment on the surface of defatted cotton through composite plant protease, which comprises the following steps: physically pretreating the defatted cotton to enhance the surface activation degree; preparing a composite enzyme solution containing bromelain, pectinase and xylanase; contacting the pretreated defatted cotton with the composite enzyme solution to carry out directional enzymolysis under controlled conditions; controlling the enzymolysis depth by adjusting the reaction temperature and pH value in stages; after the reaction is completed, the enzyme activity is terminated through heat treatment, pH adjustment or addition of chemical reagents; finally, the treated defatted cotton is cleaned and dried to obtain a surface-modified defatted cotton product, selective directional enzymolysis of the outer layer of the defatted cotton is realized, the surface fiber diameter is reduced by 8-12%, the internal fiber structure is kept intact, the tensile strength retention rate is greater than or equal to 85%, and the problem that the depth of the existing enzymolysis technology is uncontrollable is solved.

The invention discloses a Pichia pastoris indirect surface display system based on a TRAP protein scaffold, which is characterized in that Sed1p protein from saccharomyces cerevisiae is used as anchoring protein, and tetrapeptide repeat affinity protein TRAP is fixed on the anchoring protein on the surface of a Pichia pastoris cell to obtain the Pichia pastoris indirect surface display system based on the TRAP protein scaffold. The target protein with the carboxyl end fused with the peptide MEEVV or the peptide MRRVW is indirectly displayed on the surface of the pichia pastoris cell through specific binding with the tetrapeptide repeat affinity protein TRAP. The xylanase and the beta-xylosidase are indirectly displayed on the surface of the pichia pastoris cell by utilizing the surface display system, so that the pichia pastoris surface display system has the catalytic capabilities of the xylanase and the beta-xylosidase, can perform a synergistic effect to degrade the zelkova xylan, and has a good application prospect.

The invention discloses salt-resistant and alkali-resistant xylanase and application thereof, and relates to the technical field of biology. The amino acid sequence of the salt-resistant and alkali-resistant xylanase is as shown in SEQ ID NO: 2. According to the invention, a novel salt-resistant and alkali-resistant gene cwp6-xyn10 is obtained from the Qaihu saline-alkali soil by utilizing a metagenome technology. Then, the gene is subjected to heterologous expression in E.coli, and enzymatic properties are analyzed. Enzymatic characterization shows that the CWP6-Xyn10 is a salt-resistant, alkali-resistant and high-activity xylanase, and has good salt resistance and pH stability.

The invention relates to the technical field of traditional Chinese medicine compositions, and provides a traditional Chinese medicine composition for removing acnes, which is prepared from the following components in parts by weight: 1-2 parts of honeysuckle stem extract, 0.3-0.8 part of fructus forsythiae extract, 2-4 parts of radix scutellariae extract, 0.5-3.5 parts of cortex magnoliae officinalis extract and 1-5 parts of astragalus complanatus extract. The preparation method of the fructus forsythiae extract comprises the following steps: crushing fructus forsythiae, performing steam explosion treatment to obtain pretreated fructus forsythiae, adding water, adding biological compound enzyme, performing enzymolysis, filtering and performing enzyme deactivation to obtain filtrate; enabling the filtrate to pass through macroporous resin, washing with water, carrying out first elution to obtain a solution I, and carrying out second elution to obtain a solution II; performing post-treatment on the solution I to obtain forsythiaside; performing post-treatment on the solution II to obtain forsythin; the biological compound enzyme comprises alpha-galactosidase, lipase and xylanase. Through the technical scheme, the problem of low extraction rate of forsythin and forsythiaside in fructus forsythiae in the prior art is solved.

Aiming at series feed utilization problems caused by high moisture content of processing wastes of fruits and vegetables such as oranges and the like, the invention provides the dehydrating agent, the dehydrating agent comprises main components and secondary components, the main components comprise citric acid, sodium bicarbonate, sodium carbonate and quick lime, and the secondary components comprise one or more of pectinase, cellulase and xylanase; wherein the composition proportion of the main components is 1: (1-5), the composition proportion of the secondary components is 1: (1-5), and the proportion of the main components to the secondary components is 1: (0.02-0.5). Meanwhile, the invention also provides a low-cost physicochemical dehydration method and a resource utilization method of the dehydrated liquid. The method not only solves the problems that the processing wastes of fruits and vegetables such as citrus are easy to decay, high in transportation cost and difficult to crush and process, but also turns wastes into wealth, and the eluate is applied to culture of microorganisms and production and accumulation of high-value metabolites of the microorganisms, so that the comprehensive resource utilization of the processing wastes of citrus is realized; the method is helpful for realizing'grain storage in technology 'and'comprehensive and multi-way food resource development'.

This invention relates to a method for extracting plant salts from halophytes, belonging to the field of biomass resource recycling technology. The method includes five main steps: raw material pretreatment, salt extraction, ultrasonic-pressing combined desalination, filtration purification, and vacuum freeze-drying. Specifically, the salt extraction step employs a composite extraction system composed of a weakly alkaline ethanol aqueous solution, a choline-based eutectic solvent, and a combination of cellulase / pectinase / xylanase. Under gentle shaking conditions, the halophyte powder is synergistically extracted, significantly improving the dissolution rate of salts and flavor compounds. The ultrasonic-pressing combined treatment further breaks down plant tissues, enhancing the desalination effect. This invention features a simple process flow and high extraction efficiency. The resulting plant salt extract is a white or pale yellow loose porous solid, rich in minerals such as sodium and potassium, as well as various small-molecule amino acids, possessing good nutritional and flavor value, and is suitable as a natural seasoning or nutritional supplement.

The application discloses an enzymolysis method for efficiently releasing phenolic substances of rhizoma polygonati, and belongs to the field of biological enzymatic method research of rhizoma polygonati components. The rhizoma polygonati enzymolysis method comprises the following steps: crushing and screening rhizoma polygonati, weighing rhizoma polygonati powder and a composite enzyme composed of papain, beta-glucanase and xylanase, adding the composite enzyme into water, adjusting a pH value, activating in a constant-temperature water bath kettle first, then performing an enzymolysis reaction, taking out immediately after the enzymolysis is completed, inactivating, and obtaining an enzymolysis product; then adding ethanol into the enzymolysis product in a flask, performing a boiling water bath extraction, filtering while hot, obtaining an extract, performing rotary evaporation concentration, obtaining an alcohol extract, placing the precipitate in a flask, adding pure water, performing a boiling water bath extraction, filtering while hot, obtaining an extract, and obtaining a water extract, and the method ends; the method can make the phenolic components in rhizoma polygonati extraction obviously change, a plurality of new substances appear, and the new substances have good antioxidant capacity, and the method provides a scientific basis for the field of rhizoma polygonati deep processing technology.

The present disclosure relates to a modified polypeptide having xylanase activity and the use thereof.

The invention relates to a preparation method of a tobacco shaddock peel extract as well as a product and application of the tobacco shaddock peel extract. The preparation method comprises the following steps: mixing a shaddock peel raw material with compound enzyme, performing enzymolysis under the condition of microwave and ultrasonic co-treatment, filtering enzymatic hydrolysate, concentrating filtrate, performing purification and elution by adopting macroporous resin, collecting eluent, and drying to obtain the shaddock peel extract, the compound enzyme comprises rhamnosidase, cellulase, xylanase and ficin protease. According to the preparation method, a specific preparation process is adopted, efficient extraction of related active ingredients and fragrant substances in the shaddock peel can be achieved, the shaddock peel extract product with excellent fragrance characteristics is prepared, and the shaddock peel extract product can be applied to preparation of tobacco products so as to further soften smoke and improve the smoking experience.

A food or beverage ingredient is produced from enzymatically hydrolysed cereal, by contacting a slurry of cereal bran with an enzyme composition including xylanase, alpha-amylase, beta-glucanase, cellulase and endoprotease. A beverage creamer composition may contain the food or beverage ingredient.

The invention belongs to the technical field of microorganisms, and particularly relates to a fungus strain capable of efficiently degrading lignocellulose as well as a screening method and application of the fungus strain. The fungus strain is named as QMJ-1, and is classified and named as Fusarium sp. The fungus strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO: 42128, and the fungus strain is preserved on July 15, 2025. The fungus strain for degrading lignocellulose, which is obtained by the screening method disclosed by the invention, has a complete lignocellulose degrading enzyme system, including filter paper enzyme, carboxymethyl cellulase, xylanase, laccase, manganese peroxidase and the like, and various enzymes are high in activity and have the capability of efficiently degrading lignocellulose. In addition, the screening method disclosed by the invention is simple and convenient in steps and relatively low in cost, can effectively convert agricultural wastes into value-added products, and has a good application prospect.

This invention belongs to the field of agricultural fertilizer technology, specifically relating to an organic water-soluble fertilizer based on molasses fermentation concentrate and its preparation method. The organic water-soluble fertilizer comprises molasses fermentation concentrate, a compound enzyme preparation, polyaspartic acid, EDTA-2Na, and water-soluble nitrogen, phosphorus, and potassium fertilizer. It employs a staged temperature-varying enzymatic hydrolysis process. Laccase first oxidizes and degrades polyphenols at 30–35°C, opening the polyphenol-polysaccharide complex structure. Then, the temperature is raised to 42–48°C, where β-glucanase, xylanase, and mannanase directionally degrade colloidal polysaccharides. Simultaneously, EDTA-2Na is introduced to chelate metal ions, and polyaspartic acid inhibits scale dispersion. These three components synergistically form an "enzymatic hydrolysis-chelation-scale inhibition" anti-clogging system. This invention addresses the technical problems of existing molasses fermentation concentrate fertilizers being prone to clogging in drip irrigation systems, having low efficiency in constant-temperature synchronous enzymatic hydrolysis, and having an imperfect single anti-clogging mechanism. It provides an organic water-soluble fertilizer composed of molasses fermentation concentrate, a compound enzyme preparation, polyaspartic acid, EDTA-2Na, and water-soluble nitrogen, phosphorus, and potassium fertilizer. This invention effectively solves the problem of clogging of honey fermentation concentrate in drip irrigation systems, improves enzymatic hydrolysis efficiency, and is suitable for drip irrigation fertilization of various crops such as bananas and vegetables.