680 results about "Xylanase" patented technology

The invention relates to the technical field of animal feed preparation, in particular to a functional feed additive for ruminants and a preparation method of the functional feed additive for the ruminants, and the functional feed additive for the ruminants is characterized by consisting of the following components in percentage by mass: probiotics which are saccharomycetes, bacillus subtilis and enterococcus faecalis; an enzyme preparation, xylanase and protease; prebiotics are added; a microcapsule coating material; a binder; the balance is a carrier; the probiotics and the enzyme preparation are wrapped in the chitosan / sodium alginate composite film through a microcapsule coating technology, adverse factors such as temperature, humidity and illumination in the production, transportation and storage process can be effectively isolated, meanwhile, in the rumen low-pH, strong-protease and complex-microorganism environment, the coating layer can slowly release and protect active ingredients, and the effect of preventing and treating the probiotics and the enzyme preparation is achieved. Effective content and activity during final feeding are ensured, consistent growth promotion can be kept in different varieties, weights and feeding stages, and production benefits and feeding stability are improved.

The invention discloses a high temperature resistant xanthan gum lyase mutant and a preparation method thereof, wild type xanthan gum lyase comes from Paenibacillus anensis, site-directed mutagenesis is carried out on the wild type xanthan gum lyase to obtain the high temperature resistant xanthan gum lyase mutant, and the mutant comprises at least one of N66V, R292A and S676V. The enzymatic activity of the xanthan gum lyase mutant treated in a buffer solution with the pH value of 5.0 at the temperature of 65-85 DEG C for 2 h is remarkably improved compared with that of an original enzyme, and the enzymatic activity of N66V / R292A / S676V is improved by 3.8 times compared with that of the original enzyme at the temperature of 85 DEG C.

The invention discloses a compound enzyme preparation for improving the absorption and utilization rate of a fertilizer and a preparation method thereof, and belongs to the technical field of enzyme preparations. The compound enzyme preparation is prepared from the following components in parts by weight: 100 parts of purified water, 6 to 8 parts of modified cellulase, 10 to 12 parts of immobilized protease, 10 to 12 parts of phosphatase, 10 to 14 parts of urease, 8 to 10 parts of nitrate reductase, 10 to 12 parts of pectinase, 8 to 10 parts of chitinase, 6 to 8 parts of xylanase, 10 to 12 parts of beta-glucosidase, 10 to 12 parts of superoxide dismutase and 4.5 to 6 parts of a microbial agent, the modified cellulase is obtained by chemically modifying cellulase; the immobilized protease is prepared by the following steps: adsorbing activated and coupled modified polylactic acid and a protease solution, crosslinking with glutaraldehyde, filtering, washing and drying. The compound enzyme preparation improves the fertilizer absorption and utilization rate of plants.

The invention relates to bacillus velezensis and application thereof in preparation of a multifunctional straw decomposition agent, and belongs to the technical field of microorganisms. The bacillus velezensis has the functions of producing cellulase, producing xylanase, degrading abamectin, decomposing inorganic phosphorus and degrading straws; when straw is returned to the field, the multifunctional straw decomposition agent provided by the invention not only can accelerate the decomposition speed of straw, decompose abamectin residues in soil and improve the soil ecology, but also can promote the growth of pepper plants and enhance the stress resistance of the pepper plants, and has a good comprehensive application effect.

The invention relates to the field of enzyme engineering, in particular to modified xylanase, a DNA (deoxyribonucleic acid) molecule, a recombinant plasmid and a transfected cell. The modified xylanase protected by the application is formed by replacing a deletion segment of a receptor enzyme with a substitution segment, the receptor enzyme is wild type xylanase, the sequence of the receptor enzyme is SEQ ID NO: 1, the deletion segment comprises a carbohydrate binding module of the receptor enzyme, the substitution segment is a carbohydrate binding module of a donor enzyme, the donor enzyme is a carbohydrate active enzyme, and the donor enzyme is a carbohydrate active enzyme. The sequence of the donor enzyme is one of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4 and SEQ ID NO: 5. The carbohydrate binding module of the carbohydrate active enzyme with relatively high activity is replaced into the receptor enzyme, so that the binding capacity of the receptor enzyme and the carbohydrate is improved, and the activity of the receptor enzyme is improved.

The present disclosure relates to a process for preparing an upcycled material from a dry raw material with a moisture content of 0-30% w / w comprising lignocellulose and derived from spent cereal and / or pseudocereal grain and / or food waste or lignocellulosic biomass or a mixture thereof, the process comprising: a) a pre-treatment comprising the steps of: i) grinding and / or pulverizing the raw material to obtain a powder capable of passing through a sieve having a mesh size of 10-1000 μm and ii) performing autohydrolysis, followed by b) enzymatic hydrolysis of the of powder obtained from step ii), wherein the enzymatic hydrolysis comprises adding an enzyme mixture comprising at least a glucanase and a xylanase, and c) a post-treatment comprising the step of grinding and / or pulverizing the enzymatically hydrolysed mixture obtained from step b) to obtain the upcycled material in the form of a flour-like product.

The invention relates to a production process of a high-elasticity heat-resistant polyester yarn, which comprises the following steps: firstly, selecting high-molecular-weight polyethylene glycol terephthalate, adding 1-3% of nano filler and 5-10% of functional copolymer, and drying at 150 DEG C for 4-6 hours to improve the stability and processability of the material; secondly, melt extrusion is conducted at 280-290 DEG C, spinning is conducted through a multi-section temperature control spinning head, and the fiber cooling uniformity is effectively controlled. Then, two times of drafting and a heat setting process are adopted, the orientation degree of a molecular chain is improved in one time of drafting, and low-temperature plasma treatment is combined to optimize the surface activity and the setting effect of the fibers. In the secondary drafting, the fiber deformation is finely adjusted at low temperature, the thermal damage is reduced, the elasticity is improved, and finally, xylanase treatment and ultraviolet curing are combined, so that the softness, the skin-friendly property and the weather resistance of the fiber are remarkably improved.

The invention discloses a multifunctional cellulolytic enzyme and application thereof in degradation of agricultural by-products, and belongs to the technical field of biology. The multifunctional cellulolytic enzyme is named RuCelI, and the amino acid sequence of the multifunctional cellulolytic enzyme is as shown in SEQ ID NO. 1. Based on a rumen microbial metagenome resource mining technology, a novel multifunctional cellulose decomposition enzyme RuCelI is developed, and the enzyme has double activities of endo-cellulase and xylanase, can be used for degrading agricultural by-products, reducing the treatment cost and improving the degradation efficiency, and is beneficial to improving the utilization rate and the economic benefit of the agricultural by-products.

The present invention is directed toward a method for producing a plant-based food ingredient, comprising treating a slurry of plant material with an alpha amylase, a xylanase, and optionally at least one additional enzyme. The invention further includes the plant-based food ingredient produced by this method. The plant-based food ingredients produced by this method may be used to produce dairy alternative food products.

The invention discloses salt-tolerant xylanase and application thereof, and relates to the technical field of biology. The amino acid sequence of the salt-tolerant xylanase is as shown in SEQ ID NO: 1. According to the invention, a new xylanase gene xynaes is separated from a metagenome of Xinjiang Aiding Lake, and the xylanase belongs to the GH10 family and has low homology with reported xylanase. The recombinant xylanase has high activity and tolerance at a high temperature, has good performance in an alkaline environment, has high tolerance to various metal ions, quickly loses activity under SDS treatment, and should be prevented from being in contact with SDS in industrial production.

A variant polypeptide of xylanase, and a fusion protein and an enzyme composition comprising the variant polypeptide, a recombinant host cell for production of the variant polypeptide, methods for using it and its use, is disclosed comprising an amino acid sequence having at least 79%, but less than 100% amino acid sequence identity with amino acids 1-191 of SEQ ID NO: 1, wherein the amino acid sequence has xylanase activity, at least one disulfide bridge between two Cys residues in the 1-191 amino acid region, and an amino acid substitution at the position 23 or 28, or at the positions 23 and 28, the positions corresponding to the positions 23 and 28 of the SEQ ID NO: 1.

The invention provides a modification method of a ramie fabric, which comprises the following steps: sequentially treating and modifying the ramie fabric by three stages of pectinase, complex enzyme comprising xylanase, hemicellulase, cellulase and laccase, and alkaline protease, so that hairiness on the surface of the ramie fabric is greatly reduced, and the scratchiness is greatly reduced. The wearing experience feeling of the textile taking the ramie fabric as the raw material is greatly improved.

The invention discloses a radix pseudostellariae extract with high content of radix pseudostellariae cyclic peptide B and a preparation method and cosmetic application of the radix pseudostellariae extract, and belongs to the technical field of cosmetics. The preparation method comprises the following steps: (1) crushing radix pseudostellariae, adding alcohol and performing ultrasonic treatment to obtain feed liquid; (2) adding a compound enzyme into the feed liquid to carry out enzymolysis, enzyme deactivation, solid-liquid separation, concentration and impurity removal to obtain an enzymatic hydrolysate; and (3) eluting the enzymatic hydrolysate with macroporous resin to obtain an eluent, and drying to obtain the product, the compound enzyme is prepared from pectinase, papain, beta-glucosidase, cellulase and xylanase according to the mass ratio of (5 to 10) to (0.5 to 1) to (3 to 7) to (0.3 to 1). The radix pseudostellariae extract disclosed by the invention is prepared by adopting an enzymolysis process, more active ingredients can be reserved, the content of radix pseudostellariae cyclic peptide B in the obtained extract is high, and the radix pseudostellariae extract has relatively good anti-inflammatory, soothing, repairing and anti-aging effects.

The invention provides Aspergillus tubingensis HNZY02, a method for producing enzymes by the Aspergillus tubingensis HNZY02 and application of the Aspergillus tubingensis HNZY02, and belongs to the technical field of microorganisms, the Aspergillus tubingensis HNZY02 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.42148. The aspergillus tubingensis HNZY02 shows excellent cellulase and xylanase synthesis capability, can synergistically and efficiently degrade main structural components such as cellulose, hemicellulose and lignin in tobacco stems, and improves the safety and application value of cigarettes while breaking the compact structure and increasing the reducing sugar content.

The invention relates to recombinant trichoderma reesei as well as a preparation method and application thereof, and belongs to the technical field of gene engineering and enzyme engineering. The recombinant trichoderma reesei comprises a promoter and a xylanase gene, the promoter is derived from trichoderma reesei, and the xylanase gene is derived from penicillium oxalicum. Trichoderma reesei is transformed by using a xylanase gene expression plasmid, heterologous expression of xylanase from penicillium oxalicum is realized, and feruloyl oligosaccharide is prepared by using the obtained xylanase and taking brewer's grains as a raw material, so that the feruloyl oligosaccharide has high yield.

The invention belongs to the technical field of feed additives, and particularly provides a compound preparation for improving the digestibility of dry coconut shreds, which comprises a compound enzyme preparation, the compound enzyme preparation comprises xylanase with the enzyme activity of 0.5 million U / g to 25 million U / g, glucanase with the enzyme activity of 0.8 million U / g to 200 million U / g, mannase with the enzyme activity of 10,000 U / g to 200 million U / g, cellulase with the enzyme activity of 1.2 million U / g to 25 million U / g, pectinase with the enzyme activity of 10,000 U / g to 25 million U / g, galactosidase with the enzyme activity of 10,000 U / g to 300 million U / g and phytase with the enzyme activity of 0.8 million U / g to 200 million U / g. According to the compound preparation provided by the invention, macromolecular substances such as starch in the dry coconut shreds can be degraded into micromolecular substances which are easy to digest, so that the digestion utilization rate is greatly increased; fat is degraded, fatty acid components such as palmitic acid, oleic acid and lauric acid in the coconut shreds are released, and the effects of reducing blood pressure, promoting digestion and the like are achieved; meanwhile, the generated active substances can improve the disease resistance of animals, improve the health state of organisms, improve the nutritional value of the dried shredded coconut and increase the variety of unconventional feed raw materials.

The invention relates to bacillus subtilis Y32, and the preservation number of the bacillus subtilis Y32 is CGMCC (China General Microbiological Culture Collection Center) No.34819. The strain can produce xylanase with high temperature resistance and high stability, various hydrolases such as beta-mannase, protease and the like, exopolysaccharides and botrytis cinerea inhibition, and is resistant to acid, swine bile salt and 10% NaCl. The optimum temperature of xylanase produced by the strain is 55 DEG C, the optimum pH value is 6.0, under the conditions, the half-life period is 12.5 h, the relative enzyme activity is still kept above 80% after incubation for 3 h, and the xylanase has good thermal stability. The strain can also improve the yield of cucumbers and pinellia ternate, and can also improve the laying rate of 70-week-old hyline brown laying hens from 70.4% to 90.1%. The strain has a good application prospect in the fields of agriculture, food processing and cosmetic production.

The invention relates to bacillus megatherium XW-02 (the preservation number is CCTCC NO: M 2025185), a microbial preparation of the bacillus megatherium XW-02, and application of the microbial preparation. The bacillus megatherium XW-02 has the advantages that the preservation number is CCTCC NO: M 2025185; the strain can efficiently express xylanase through xylan induction, hemicellulose in tobacco leaves is remarkably degraded, chemical composition is optimized, and combustion harmful substances are reduced. The treated cigar has the advantages that the aroma richness is improved, the purity is improved, the taste mellowness is improved, and the overall quality is improved by 1-2 grades. The xylan extracted from agricultural wastes is used as an inducer to construct a green biological manufacturing system, the cost is reduced by more than 80% compared with the traditional process, and the emission of three wastes is reduced. The process compatibility is high, the method can be matched with an existing production line only by additionally arranging a strain culture module, the single-line annual tobacco leaf treatment amount reaches 500 tons, and economic benefits are remarkable. The invention provides a safe, efficient and environment-friendly quality improvement solution for the tobacco industry, and has great industrialization value.

The invention relates to the field of extraction of sacha inchi oil, in particular to a preparation method and application of sacha inchi oil, and aims to solve the problems of large loss of heat-sensitive components, solvent residue and low oil yield in the traditional process. The ratio of cellulase to pectinase to xylanase to lipase is 3: 2: 1: 0.5, and carrying out enzymolysis for 2-3 hours at the pH value of 4.5-5.5 and the temperature of 45-50 DEG C; the obtained enzymolysis product is subjected to 28-40kHz ultrasonic treatment for 20-30 minutes, and then is subjected to two-stage gradient squeezing (pre-squeezing at 50-55 DEG C to final squeezing at 35-40 DEG C) in combination with 0.1 mu m ceramic membrane filtration, so that efficient oil extraction and active ingredient protection are realized. The oil yield of the sacha inchi oil is increased to 50.0%, the content of alpha-linolenic acid is larger than or equal to 42%, the content of vitamin E reaches 158 mg / 100 g, no solvent is left, and the sacha inchi oil is suitable for the fields of functional food and cosmetics.

The invention discloses chimeric xylanase XynZT-1-Z5 and a construction method thereof, and relates to the technical field of molecular biology, and the technical key points are as follows: comparing the sequence of xylanase XynZT-1 with the sequence of xylanase from different thermoduric bacteria, analyzing the amino acid conserved site of the xylanase XynZT-1, and replacing the sequence beta5-beta6 of the thermoduric bacteria onto the XynZT-1 to obtain the chimeric xylanase XynZT-1-Z5. The chimeric enzyme XynZT-1-Z5 has efficient overexpression, good solubility and relatively strong temperature and pH stability, is particularly suitable for industrial enzyme catalytic reaction under the conditions of high temperature and extreme pH, and can be widely applied to the food industry, the wine brewing industry, the papermaking industry and the feed industry.

The invention belongs to the technical field of preparation of food additives, and particularly relates to a method for extracting sa-hao seed gum and oligosaccharide thereof by using a compound enzyme method. According to the method provided by the invention, complete artemisia desertorum seeds are taken as a raw material, enzymolysis is carried out by using a compound system consisting of xylanase, cellulase and pectinase, and after enzyme deactivation and alcohol precipitation, the artemisia desertorum gum or the artemisia desertorum gum oligosaccharide can be obtained. Compared with a traditional water extraction method and an acid-base extraction method, the extraction yield is remarkably increased through the enzymolysis synergistic effect, the process is green and safe, no chemical residue exists, the molecular weight of the product is controllable, and high purity and functional activity are both achieved. The method is easy and convenient to operate, low in cost and suitable for large-scale production, and has wide application prospects in the fields of food, medicine and functional oligosaccharide development.

The invention discloses an extracellular vesicle and a preparation method and application thereof, and belongs to the technical field of cosmetics, and the preparation method comprises the following steps: mixing rose and a PBS buffer solution, juicing, and filtering to obtain a filtrate; adding cellulase, xylanase and beta-glucanase into the filtrate, carrying out enzymolysis, and filtering to obtain an enzymatic hydrolysate; and centrifuging the enzymatic hydrolysate, filtering, resuspending and precipitating to obtain the rose extracellular vesicles. The rose extracellular vesicles can guarantee structural integrity and biological activity, can effectively load active ingredients, and can effectively deliver active molecules in the rose extracellular vesicles to target cells through interaction with membranes of skin cells. The extracellular vesicles can be fused with skin cell membranes to enter cells, active ingredients encapsulated by the extracellular vesicles are released, and the absorption efficiency of skin is improved.

The invention relates to an enzyme and bacterium combined feed additive for pre-digestion of feed raw materials as well as preparation and application of the enzyme and bacterium combined feed additive. The feed additive comprises: (a) an enzyme preparation, which is composed of protease, cellulase, xylanase, mannase, phytase, pectinase and keratinase, and has a total enzyme activity of 42500-45000 U / g; the probiotics are composed of bacillus subtilis, lactobacillus plantarum and saccharomyces cerevisiae, and the viable count is larger than or equal to 107 CFU / g; and (c) the mass ratio of enzyme to bacteria is (1-2): 1. According to the additive, the enzyme preparation and the probiotics are jointly used, and the raw materials are effectively pretreated, so that the nutritional value and the digestibility of the feed are improved. The feed additive disclosed by the invention comprises a specific enzyme preparation and probiotic combination, and predigestion treatment on the feed raw materials is realized through an optimized preparation process and fermentation process, so that the nutritional value and the utilization value of the feed raw materials are improved. The additive is simple in preparation method, low in cost and environment-friendly, and has a wide application prospect.

The invention discloses a compound bacterium enzyme preparation for ensiling and application of the compound bacterium enzyme preparation, and belongs to the field of bacterium enzyme compound preparations. The bacterial enzyme preparation disclosed by the invention comprises lactobacillus plantarum, lactobacillus buchneri, cellulase and xylanase, has the effects of improving the fermentation quality of silage, promoting the improvement of the organism immunity and the anti-stress capability of ruminants and the like, and has wide market value.

The preparation method comprises the following steps: degreasing polygonatum cyrtonema leaf superfine powder, sequentially carrying out ultrasonic-assisted enzymolysis treatment on cellulase, xylanase, high-temperature-resistant alpha-amylase, papain and saccharifying enzyme, and carrying out alcohol precipitation, dialysis and vacuum freeze drying treatment to obtain the polygonatum cyrtonema leaf polysaccharide. The polygonatum cyrtonema leaf enzyme extraction crude polysaccharide is obtained; and separating the enzyme-extracted crude polysaccharide through a cellulose column, and purifying through a sephadex column to obtain the polygonatum cyrtonema leaf enzyme-extracted polysaccharide. Polysaccharide in polygonatum cyrtonema leaves is extracted through ultrasonic-assisted compound enzyme, single pure polysaccharide is obtained through alcohol precipitation, dialysis, cellulose column chromatography and gel column chromatography, and the purity can reach 99.86%; the obtained enzyme extraction polysaccharide has good anti-cancer activity, can significantly inhibit proliferation of breast cancer cells and colorectal cancer cells, has the characteristics of good biocompatibility, strong specificity, low toxic and side effects and the like, and has wide application prospects.

The invention discloses a xylanase mutant with synchronously improved thermal stability and pH tolerance and application of the xylanase mutant, and belongs to the field of gene engineering and protein engineering. According to the invention, xylanase AbXyL10A from Agaricus bisporus var burnettii is taken as a female parent, and two amino acid sites of Thr234 and Arg268 are subjected to mutation, so that two types of xylanase mutants T234N and R268L are obtained. Compared with wild type xylanase, the thermal stability and pH tolerance of the two xylanase mutants are obviously improved. In the aspect of specific activity, when the beech xylan is used as a substrate, the specific activity of the two xylanase mutants is obviously improved compared with that of wild type xylanase. The invention provides a theoretical reference for the improvement of xylanase, and the obtained xylanase mutant with synchronously improved thermal stability and pH tolerance has a huge application prospect in the aspects of animal feed and biomass degradation sugar production.

The invention relates to a compound preparation for improving the physique of weak stiff livestock. Every 100 ml of the compound preparation mainly comprises the following components according to a ratio: 0.1 to 0.3 g of vitamin E, 1 to 3 g of lactobacillus, 1 to 2 g of bile acid, 1 to 3 g of xylanase, 0.2 to 0.4 g of vitamin B1, 0.2 to 0.4 g of vitamin B2, 0.2 to 0.4 g of vitamin B6, 0.2 to 0.4 g of vitamin B12, 1 to 3 g of nicotinamide, 0.5 to 1.0 g of butafosfan, 1 to 3 g of taurine, 1 to 3 g of lysine, 0.5 to 1.0 g of levamisole hydrochloride, 5 g of soybean oil, 50 g of gelatin liquid, 5 to 10 ml of sodium sulfate solution, 1 to 2 g of Pickering emulsifier, 3 to 5 g of liquid paraffin and 1 to 2 g of Tween-80. The compound preparation not only pays attention to nutritional supplement, but also pays attention to regulation of an immune system and improvement of intestinal flora balance, so that the physique of the weak stiff livestock is comprehensively improved, and powerful support is provided for efficient development of the breeding industry.

The invention discloses a method for cyclically preparing high-activity xylooligosaccharide from recoverable acid, and belongs to the technical field of xylooligosaccharide preparation. The method comprises the following steps: carrying out hydrolysis reaction on moso bamboo processing residues and a gallic acid solution, and carrying out solid-liquid separation to obtain a high-polymerization-degree xylooligosaccharide solution; then carrying out enzymolysis reaction on the xylooligosaccharide solution with high polymerization degree and xylanase to obtain a high-activity xylooligosaccharide solution, and carrying out concentration, refrigeration treatment and recrystallization on the obtained high-activity xylooligosaccharide solution to recover residual acid so as to obtain the high-concentration high-activity xylooligosaccharide solution. According to the method, the high-activity xylooligosaccharide is prepared through hydrolysis of the easily-recycled organic acid, and the method has the advantages of being high in xylooligosaccharide concentration, high in high-activity component content, simple in reagent recycling, easy to operate and the like and is suitable for preparing xylooligosaccharide through high-selectivity conversion of hemicellulose in bamboo processing residues.

The invention discloses a method for preparing biomechanical pulp by treating kenaf with ternary low eutectic solvent-assisted bioenzyme, comprising the following steps: cutting kenaf stalks into small segments, mixing with low eutectic solvents, reacting at 100-150°C for 30-60min, cooling, and solid-liquid separation to obtain pretreated kenaf stalks; washing the pretreated kenaf stalks to neutrality, mixing with composite enzymes, and performing enzyme treatment at 40-60°C for 30-120min, performing pulping treatment on the kenaf stalks after enzyme treatment, washing and screening the slurry to obtain biomechanical pulp; the composite enzyme is a composite enzyme of xylanase and lipase, or a composite enzyme of cellulase and xylanase. Pretreatment with a low eutectic solvent can effectively improve the treatment efficiency of the bioenzyme.

The invention discloses a method for degumming ramie by using biological enzymes, and belongs to the technical field of ramie processing. The method provided by the invention comprises the steps of degumming by using a composite enzymatic hydrolyzate of pectinase, mannanase and xylanase, alkali boiling, cleaning, bleaching and acid washing, cleaning, dehydration and the like. The weight loss rate of ramie treated by the method can reach about 47.8%, compared with the traditional chemical method, such as the weight loss rate of about 18% for alkali boiling using NaOH, the method not only significantly improves the degumming efficiency and improves the fiber quality, but also reduces the production cost and environmental pollution, and has significant technical advantages and economic benefits.