337 results about "Invertase" patented technology

The present invention provides nucleotide sequences of Aspegillus fumigatus that encode proteins which exhibit enzyme activities. Vectors, expression constructs, and host cells comprising the nucleotide sequences of the enzyme genes are also provided. The invention further provides methods for producing the enzymes, and methods for modifying the enzymes in order to improve their desirable characteristics. The activities displayed by the enzymes of the invention include those of a tannase, cellulase, glucose oxidase, glucoamylase, phytase, beta-galactosidases, invertase, lipase, alpha-amylase, laccase, polygalacturonase or xylanase. The enzymes of the invention can be used in a variety of industrial processes. Enzymatically active compositions in various forms as well as antibodies to the enzymes and fragments thereof, are also provided.

The invention provides a functional geese fermented sausage and a preparation method thereof. The preparation method of the fermented sausage is characterized by comprising the following steps: killing geese, burning the geese to remove feathers, removing viscera, washing and cleaning the geese; then taking out the white and dark meat, spreading a mixture of nitrite and sodium erythorbate on the geese, pickling the geese in a refrigerator at 0-4 DEG C 15-18 hours, mincing, performing cut-mixing and inoculating the geese; filling the geese into a sausage casing and fermenting; and roasting andcooling the sausage, thus obtaining the geese fermented sausage product with the functions of resisting oxidation, resisting angiotensin converting enzyme (ACE) and regulating immune. The preparationmethod comprises the following steps in sequence: preparing a fermenting agent, preparing the geese, pickling, rolling, performing vacuum cut-mixing, filling the geese into the sausage casing, fermenting and finally ripening, thus obtaining the functional geese fermented sausage. The invention has the following advantage: the method for preparing the fermented sausage with the functions of resisting oxidation, resisting ACE and regulating immune by utilizing the geese is disclosed for the first time.

The invention provides a portable copper ion concentration detection method based on click chemistry and belongs to the field of analytical chemistry. Under the condition that sodium ascorbate reduces cupric into cuprous as a catalyst, a 1,3-dipole cycloaddition reaction is carried out between NDA modified with an azide group and DNA modified with an alkynyl group, so that DNA modified with cane sugar invertase is fixed on magnetic beads; The magnetic beads are separated from a solution, the cleaned magnetic beads are dissolved into a cane sugar solution to carry out enzymatic hydrolysis, and then a reaction solution after enzymatic hydrolysis is measured by adopting a blood glucose meter, so that copper ion concentration can be detected. The portable copper ion concentration detection method provided by the invention takes the blood glucose meter as a platform and can portably and quickly detect the copper ion concentration on site; and the portable copper ion concentration detection method can be applied to copper ion detection in a human body serum sample, and the portable copper ion concentration detection method has the advantages of easy operation, low cost, high sensitivity and good specificity.

The invention discloses a method for portably and rapidly detecting ochratoxin A. The method comprises the following steps: first, preparing sucrose invertase-terminal alkyne modified DNA; subsequently, combining biotin-nitrine modified DNA with the surface of a magnetic bead, adding the ochratoxin A with different concentration, and mixing; adding the previously-prepared sucrose invertase-terminal alkyne modified DNA, a CuSO4 solution and an ascorbic acid solution, wherein the ascorbic acid solution can be used for reducing Cu(II) to obtain a Cu(I) compound so as to catalyze the biotin-nitrine modified DNA and the sucrose invertase-terminal alkyne modified DNA to have a click chemistry reaction; taking a supernatant liquor after the reaction is ended, then adding the supernatant liquor into a sucrose solution, wherein the sucrose invertase can effectively catalyze the sucrose in the solution to be transformed into glucose; and finally, measuring by adopting a glucometer. The method is used for organically combining the rapid reaction advantage of the click chemistry reaction with the high specificity characteristic of an aptamer, and adopting the simple and portable glucometer to acquire data, thus the operation complexity and detection cost are greatly reduced, and the sensitivity and selectivity are improved.

Nucleic acid sequences of truncated or mutated alternansucrases, vectors containing these nucleic acids sequences, host cells transformed with the nucleic acid sequences encoding truncated or mutated alternansucrases are provided. Furthermore, a process to recombinantly alternansucrase with a high level of expression, while retaining the enzymatic activity is described.

Described herein are novel biological circuit chemotactic converter that utilize modular components, such as genetic toggle switches and single invertase memory modules (SIMMs), for detecting and converting external inputs, such as chemoattractants, into outputs that allow for autonomous chemotaxis in cellular systems. Flexibility in these biological circuit chemotactic converter is provided by combining individual modular components, i.e., SIMMs and genetic toggle switches, together. These biological converter switches can be combined in a variety of network topologies to create network systems that regulate chemotactic responses based on the combination and nature of input signals received.

The present invention is directed to methods and compositions to eliminate cold storage-induced sweetening of potato or sweet potato. The invention is accomplished in part by silencing the vacuolar acid invertase gene using RNAi technology. The resulting potatoes withstand cold storage without significant hexogenesis, producing potatoes or sweet potatoes that have reduced Maillard reactions when fried in hot oil. The fried products accumulate significantly lower levels of acrylamide compared to controls.

The invention relates to commercially viable methods for producing biologically active vitamin K dependent proteins, particularly Factor IX. Factor IX is produced at a level of at least about 15 mg / L and is at least 25% biologically active. The method relies upon co-expression of one or more of paired basic amino acid converting enzyme (PACE), vitamin K dependent epoxide reductase (VKOR) and vitamin K dependent γ-glutamyl carboxylase (VKGC) at a preferred ratio so that the vitamin K dependent protein is efficiently produced and processed by a recombinant cell.

The present invention relates to the development and utilization of plant protein. Defatted wheat plumule as material is protein extracted and enzyme hydrolyzed to obtain hydrolysate containing several kinds of powerful ACE inhibiting active peptides. The obtained wheat plumule protein hydrolysate is one kind of polypeptide mixture containing Ala-Met-Tyr tripeptide, Val-Trp depeptide and other polypeptide compounds. The wheat plumule protein hydrolysate can lower blood pressure of hypertension patient by means of suppressing ACE, and is one safe and effective blood pressure lowering material. In addition, the hydrolysate has also effects of raising immunity and resisting oxidation.

The invention relates to a preparation method of a levan-containing yoghourt, and the method comprises the following steps of: (1) preparing a fermented culture solution by implementing activation and enzyme production fermentation to a levan strain; (2) centrifugally collecting the supernatant of the fermented culture solution or the thallus; (3) adding ammonium sulfate powder into the supernatant of the fermented culture solution, centrifugating and collecting albumen precipitation; or crushing and centrifugating the thallus by using ultrasonic waves, adding the ammonium sulfate powder into the supernatant, centrifugating and collecting the albumen precipitation; and purifying to prepare pure enzyme protein levansucrase; and (4) adding sugar and the pure enzyme protein levansucrase into raw milk, then vaccinating with lactobacillus, and preparing the levan-containing yoghourt after fermentation. According to the preparation method of the levan-containing yoghourt, levan is produced from microorganism levan sucrase when yoghourt is fermented, so that a novel yoghourt containing functional polysaccharide is directly acquired without complex polysaccharide purification processes. The preparation method of the levan-containing yoghourt has wide prospect of application.

The invention relates to a method for preparing a high-protein forage by utilizing waste cassava vinasse. The method comprises the steps: 1) utilizing rotation heating evaporation to concentrate a cassava vinasse liquid; 2) adding a probiotics seed liquid and invertase into the cassava vinasse concentrated liquid, uniformly stirring and fermenting; 3) putting the cassava vinasse slurry into a diaphragm type filter press to perform positive-pressure water draining, extruding water draining, residual liquid evacuating and air back flushing, and collecting filtrate; 4) putting vinasse filter cakes in a drying apparatus to perform high-temperature ventilation drying; 5) stirring the filtrate, corn flour, wheat bran fine powder, peanut bran and soybean meal to mix uniformly; and 6) grinding dry filter cakes into granular vinasse, uniformly mixing granular vinasse and dry regulators, preparing into granules, and drying to obtain the high-protein forage. The method is low in production cost and helps to reduce environmental pollution and change waste into valuables, and the prepared forage product is high in nutrition value, abundant in beneficial bacteria, free of hormones and free of antibiotics.

The invention relates to a binding solution for purification of nucleic acid. The binding solution particularly comprises salts and solubilizers; the salts are selected from at least one of potassium chloride, potassium acetate, lithium chloride, lithium acetate, sodium chloride and sodium acetate; the solubilizers are nonionic solubilizers and / or ionic solubilizers. A method of separating and purifying nucleic acid via the binding solution is simple to perform and has no need for centrifuging, the target nucleic acid purified has good purity and is directly applicable to linking, transformation, enzyme cleavage, sequencing, hybridizing and other molecular biological experiments.

The invention provides an albumin angiotensin converting enzyme inhibition peptide and a preparation method thereof, belonging to the biotechnology field. A new active peptide with angiotensin converting enzyme inhibition function is separated and purified from albumin zymolyte and has a sequence of RVPSL. The preparation method comprises the steps of: carrying out the enzymolysis on albumin to prepare the zymolyte, purifying the albumin angiotensin converting enzyme inhibition peptide, identifying the structure of the albumin angiotensin converting enzyme inhibition peptide, synthesizing the albumin angiotensin converting enzyme inhibition peptide and checking the activity of the albumin angiotensin converting enzyme inhibition peptide. The invention can be used for preparing antihypertensive drugs and functional foods assisting blood pressure reduction.

The invention aims at providing a novel angiotensin-converting enzyme inhibition peptide, namely an ACE inhibition peptide prepared form isochrysis galbana, and with the amino acid sequence of SEQ ID NO:1. The invention provides a preparation method of a high-activity angiotensin-converting enzyme inhibition peptide sourcing from marine microalgae. The current angiotensin-converting enzyme inhibiter mainly comes from food and large macroalgae of laver and the like, and coming from bait microalgae is not reported. The structure of the obtained active peptide with a unique structure is Tyr-Met-Gly-Leu-Asp-Leu-Lys. The structure is novel and has the characteristics of being small in molecular weight and easily absorbed by a human body.

The invention belongs to the technical field of natural product preparation and particularly relates to a method for preparing high-activity corn antihypertensive peptide and a special device. An enzyme membrane reactor comprises a reaction tank, a temperature control system, an automatic pH dripping instrument, a strong force stirrer, a material liquid storage tank, a peristaltic pump, a control valve, a pressure meter, an ultrafiltration membrane assembly, a pipeline heat exchanger, a collection tank, a liquid level controller, a nanofiltration membrane assembly and the like. The process method comprises the following steps that corn gluten meal is subjected to alcohol alkali liquid extraction to obtain concentrated corn protein, the concentrated corn protein is pretreated at high temperature and is then added into a continuous enzyme membrane reactor system, the corn antihypertensive peptide is prepared by adopting the enzyme membrane coupling technology, then, the products are subjected to ultrafiltration grading through the ultrafiltration membrane assembly and then are subjected to desalting through the nanofiltration membrane, and the high-activity corn antihypertensive peptide is obtained. The inhibitory activity of the obtained corn antihypertensive peptide on angiotensin-converting enzyme (ACE) is maintained at a value higher than 86 percent, the protein recovery rate reaches higher than 70 percent, and in addition, the systolic pressure of spontaneously hypertensive rats can be obviously reduced.

The invention relates to a method for detecting a transcription factor by a biosensor and a glucometer. The method comprises the following steps of 1, fixing a nucleic acid sequence specifically binding to a transcription factor on a solid matrix, and adding a sample into the solid matrix with the nucleic acid sequence to obtain a compound, 2, fixing a transcription factor antibody and invertase on carriers, 3, mixing the solid matrix obtained by the step 1 and the carriers obtained by the step 2, and removing the unbound carriers, and 4, adding excess cane sugar into the mixture obtained by the step 3, carrying out a reaction for a certain time, detecting glucose content by a conventional glucometer, and converting the glucose content into content of the transcription factor in the sample. The method has high sensitivity and singularity, simple processes, a low cost and a fast detection rate, adopts the portable detection apparatuses, and is suitable for popular popularization.

The invention relates to a saline alkali land improved special compound fertilizer for improving saline alkali lands and growing crops in the saline alkali lands, which is characterized in that the saline alkali land improved special compound fertilizer comprises 15 kilograms of devulcanized slag, 8 kilograms of bentonite, 25 kilograms of monoammonium phosphate, 27 kilograms of urea, 21 kilograms of potassium chloride or potassium sulfate and 4 kilograms of zinc sulfate as calculated by 100 kilograms of compositions; and the special compound fertilizer can provide organic nutritive compositions such as medium elements, microelements and humic acid, improve the quality of the crops, increase the reserve of soil nutrients, improve the effectiveness of nitrogen, phosphorus, potassium and the microelements, have important function on the balance of soil nutrient resources in the saline alkali lands, update and activate old organic substances, and improve the quality of humic substances, thereby comprehensively improving the soil fertility, the utilization rate of the nitrogen, and the activity of various enzymes such as invertase, protease and amylase in the soil, reducing ammonia nitration, promoting phosphorus absorption of the crops, reducing environmental pollution, contributing to environmental protection, and reducing the amount of the fertilizer, consequently saving the fertilization cost.

The invention provides a halohydrin dehalogenase encoded gene, a recombinant vector containing the gene, and a recombinant genetically engineered bacterium, and provides a novel bacterial strain, namely parvibaculum lavamentivorans ZJB 14001, of the gene. The recombinant halohydrin dehalogenase is regarded as an invertase to synthesize epoxy chloropropane by taking catalysis of 1,3-dichloro-2-propanol as an example, to prepare (S)-4-chloro-3-hydroxybutyronitrile by taking catalysis of CN-mediated (S)-epoxy chloropropane with a ring-opening reaction as an example, and to respectively prepare (S)-4-chloro-3-hydroxybutyronitrile and ethyl-(R)-4-cyano-3-hydroxybutyrate by taking catalysis of 1,3-dichloro-2-propanol and (S)-4-chloro-3-hydroxybutyronitrile as an example with CN-mediated ring opening.

The invention relates to a preparation method of a biosensor for detecting L-histidine and application thereof. Firstly, single strand DNA1 modified by biotin is fixed on magnetic beads (MBs) modified by streptavidin (SA) to obtain a compound DNA1-MBs. In addition, colloidal gold is modified by DNA2 and invertase to obtain DNA2-invertase modified colloidal gold. Then, DNA1-MBs and DNA2-invertase modified colloidal gold are mixed to obtain an L-histidine sensor. A certain amount of L-histidine is added into the prepared L-histidine sensor to react for a period of time, through magnetic separation, a supernatant is obtained, saccharose is added into the supernatant to react for a period of time and is converted to glucose by invertase, generated glucose is directly detected by a glucometer, and the content of L-histidine is detected by detecting the content of glucose. The sensor is high in sensitivity and selectivity; the glucometer is used as a detection instrument and the magnetic beads are used as carriers; the operation is simple; the detection instrument is small in size, low in price and convenient to carry.

The invention provides escherichia.coli with high L-aspartase yield. The collection number of the escherichia.coli collected in the China General Microbiological Culture Collection Center is CGMCC No.5450. The activity of a shake flask-fermented biotransformation enzyme (L-aspartase) can be up to 4,200 U / ml; the activity of a biotransformation enzyme fermented with a 2-ton tank can be up to 4,500 U / ml, which is increased by over 30 percent in comparison to the activity of a fermentation strain; and fumaric acid is biologically transformed directly and efficiently by using free cells with high transformation activities to generate L-aspartase; and when the concentration of fumaric acid is 18.5-20.0 percent, the biological transformation time of a 20-ton tank is shortened by one third in comparison to strain deriving time, and is 6-8 hours, the transformation rate is over 97.5 percent, and the transformation efficiency is higher that of the same level.

The invention relates to a starch surface glue sizing agent, a method for producing the same and application thereof. The method is as follows: starch is subjected to dissolving, conversion, enzyme deactivation, storage and feeding. The glue sizing agent produced by the method has the characteristics of stable quality, low preparation cost and the like.

The invention discloses a synthetic method of 11alpha hydroxy-canrenone, comprising the following steps of: heating and dissolving canrenone, propylene glycol and tween 80 to obtain a substrate; adding the substrate into a culture medium; adding an apergillus ochraceus strain and fermenting; and after the fermenting is finished, extracting and refining from a mycelium to obtain the 11alpha hydroxy-canrenone. By adopting a biosynthesis technology, novel invertase can be selected, thereby greatly reducing the cost of the 11alpha hydroxy-canrenone. The specificity of the invertase on the canrenone is improved to over 85 percent from the traditional 75 percent; the feeding concentration of the canrenone is improved to over 0.8 percent from 0.6 percent; the yield of the 11alpha hydroxy-canrenone is improved to over 60 percent from 50 percent; and main quality indexes of the 11alpha hydroxy-canrenone reach that: the content (HPLC-High Performance Liquid Chromatography) is not less than 98 percent, the content of total impurities is not more than 2.0 percent and the content of single impurity peak is not more than 1.5 percent.

A method of using high efficiency chromatograph and mass spectrum unitedly to quickly screen inhibitor of angiotonin converzyme uses hippuric acid bialkyd as substrate and uses purified rabbit lung angiotonin converzyme as source of angiotonin converzyme for carrying out quick analysis on vitality of angiotonin converzyme in human blood plasma.

The invention provides a co-production method of high-purity fructose-glucose powder by using fructo-oligosaccharide. The method comprises the following steps: (1) preparing materials; (2) transforming immobilized enzyme; (3) decoloring and filtering; (4) hybridizing; (5) performing chromatographic separation; (6) separating mother liquor; (7) performing invertase enzymolysis; (8) perfoming isomerization; (9) preparing fructose-glucose syrup; (10) drying; and (11) preparing the fructose-glucose powder, wherein fructosyl transferase which is immobilized by adopting an alumina-carrageenan-glutaraldehyde crosslinking immobilization technology is transformed, and high-purity fructo-oligosaccharide is produced by adopting a chromatographic separation technology, and separated mother liquor containing monosaccharide and cane sugar as main components is generated; the mother liquor has complex components, cannot be utilized in a general state, and is directly drained, so that resource waste and environment pollution are caused. Therefore, the cane sugar and little fructo-oligosaccharide in the separated mother liquor are subjected to invertase enzymolysis, fructose-glucose is produced by refining and isomerizing, and the fructose-glucose powder is prepared by adopting an advanced vacuum drying technology, so that the comprehensive utilization of the fructo-oligosaccharide is realized, and the considerable economical benefit is obtained.

The invention discloses an efficient agaricus bisporus culture medium prepared from crop straws and vinasse, wherein the culture medium comprises the following components: 36-44 parts by weight of wheat straw, 20-30 parts by weight of rice straw, 37-43 parts by weight of vinasse, 9-11 parts by weight of soybean cake powder, 1-2 parts by weight of cellulase, 0.8-1 part by weight of xylanase, 0.1-0.3 part by weight of Aspergillus oryzae, 0.3-0.5 part by weight of Aspergillus niger, 1.2-1.4 parts by weight of candida utilis, 1.1-1.3 parts by weight of bacillus subtilis, 1-2 parts by weight of solid lactobacillus powder, 2-3 part by weight of zeolite, and 1-2 part by weight of kieselguhr. The activation and biochemical invertase treatments are applied to the straws for preparing highly soluble straws; then mixing the straws with the fermented soybean cake for preparing highly active biochemical humic acid through high-temperature stirring reaction; therefore, the culture medium is conveniently absorbed and utilized by the agaricus bisporus, so as to accelerate the metastasis of the agaricus bisporus. The conditions of generating intermediate products of the biochemical humic acid are created by loading the zeolite and the kieselguhr to the biochemical invertase and the zymophyte.

Disclosed is an apparatus and method for continuously converting sucrose to β-D-glucose. The method comprises a three stage enzymatic reactor in which an aqueous solution of sucrose is first converted into a solution of fructose and α-D-glucose by passing it through a porous, packed column containing an inert media on which invertase is immobilized. This solution is then sent through a second packed column containing glucose isomerase and finally a third packed column containing mutarotase. Solution temperature and pH are adjusted to maximize glucose output.

A series of independent non-transgenic mutations found in acid invertase genes of tomatoes; tomato plants having these mutations in their acid invertase genes; and a method of creating and finding similar and / or additional mutations in acid invertase genes by screening pooled and / or individual tomato plants. The tomato plants of the present invention exhibit altered acid invertase enzyme activity and altered concentrations of sugar in the tomato fruit without having the inclusion of foreign nucleic acids in their genomes.