326 results about "Invertase" patented technology

The invention provides a functional geese fermented sausage and a preparation method thereof. The preparation method of the fermented sausage is characterized by comprising the following steps: killing geese, burning the geese to remove feathers, removing viscera, washing and cleaning the geese; then taking out the white and dark meat, spreading a mixture of nitrite and sodium erythorbate on the geese, pickling the geese in a refrigerator at 0-4 DEG C 15-18 hours, mincing, performing cut-mixing and inoculating the geese; filling the geese into a sausage casing and fermenting; and roasting andcooling the sausage, thus obtaining the geese fermented sausage product with the functions of resisting oxidation, resisting angiotensin converting enzyme (ACE) and regulating immune. The preparationmethod comprises the following steps in sequence: preparing a fermenting agent, preparing the geese, pickling, rolling, performing vacuum cut-mixing, filling the geese into the sausage casing, fermenting and finally ripening, thus obtaining the functional geese fermented sausage. The invention has the following advantage: the method for preparing the fermented sausage with the functions of resisting oxidation, resisting ACE and regulating immune by utilizing the geese is disclosed for the first time.

The invention provides a portable copper ion concentration detection method based on click chemistry and belongs to the field of analytical chemistry. Under the condition that sodium ascorbate reduces cupric into cuprous as a catalyst, a 1,3-dipole cycloaddition reaction is carried out between NDA modified with an azide group and DNA modified with an alkynyl group, so that DNA modified with cane sugar invertase is fixed on magnetic beads; The magnetic beads are separated from a solution, the cleaned magnetic beads are dissolved into a cane sugar solution to carry out enzymatic hydrolysis, and then a reaction solution after enzymatic hydrolysis is measured by adopting a blood glucose meter, so that copper ion concentration can be detected. The portable copper ion concentration detection method provided by the invention takes the blood glucose meter as a platform and can portably and quickly detect the copper ion concentration on site; and the portable copper ion concentration detection method can be applied to copper ion detection in a human body serum sample, and the portable copper ion concentration detection method has the advantages of easy operation, low cost, high sensitivity and good specificity.

Described herein are novel biological circuit chemotactic converter that utilize modular components, such as genetic toggle switches and single invertase memory modules (SIMMs), for detecting and converting external inputs, such as chemoattractants, into outputs that allow for autonomous chemotaxis in cellular systems. Flexibility in these biological circuit chemotactic converter is provided by combining individual modular components, i.e., SIMMs and genetic toggle switches, together. These biological converter switches can be combined in a variety of network topologies to create network systems that regulate chemotactic responses based on the combination and nature of input signals received.

The present invention is directed to methods and compositions to eliminate cold storage-induced sweetening of potato or sweet potato. The invention is accomplished in part by silencing the vacuolar acid invertase gene using RNAi technology. The resulting potatoes withstand cold storage without significant hexogenesis, producing potatoes or sweet potatoes that have reduced Maillard reactions when fried in hot oil. The fried products accumulate significantly lower levels of acrylamide compared to controls.

The invention relates to a preparation method of a levan-containing yoghourt, and the method comprises the following steps of: (1) preparing a fermented culture solution by implementing activation and enzyme production fermentation to a levan strain; (2) centrifugally collecting the supernatant of the fermented culture solution or the thallus; (3) adding ammonium sulfate powder into the supernatant of the fermented culture solution, centrifugating and collecting albumen precipitation; or crushing and centrifugating the thallus by using ultrasonic waves, adding the ammonium sulfate powder into the supernatant, centrifugating and collecting the albumen precipitation; and purifying to prepare pure enzyme protein levansucrase; and (4) adding sugar and the pure enzyme protein levansucrase into raw milk, then vaccinating with lactobacillus, and preparing the levan-containing yoghourt after fermentation. According to the preparation method of the levan-containing yoghourt, levan is produced from microorganism levan sucrase when yoghourt is fermented, so that a novel yoghourt containing functional polysaccharide is directly acquired without complex polysaccharide purification processes. The preparation method of the levan-containing yoghourt has wide prospect of application.

The invention provides an albumin angiotensin converting enzyme inhibition peptide and a preparation method thereof, belonging to the biotechnology field. A new active peptide with angiotensin converting enzyme inhibition function is separated and purified from albumin zymolyte and has a sequence of RVPSL. The preparation method comprises the steps of: carrying out the enzymolysis on albumin to prepare the zymolyte, purifying the albumin angiotensin converting enzyme inhibition peptide, identifying the structure of the albumin angiotensin converting enzyme inhibition peptide, synthesizing the albumin angiotensin converting enzyme inhibition peptide and checking the activity of the albumin angiotensin converting enzyme inhibition peptide. The invention can be used for preparing antihypertensive drugs and functional foods assisting blood pressure reduction.

The invention aims at providing a novel angiotensin-converting enzyme inhibition peptide, namely an ACE inhibition peptide prepared form isochrysis galbana, and with the amino acid sequence of SEQ ID NO:1. The invention provides a preparation method of a high-activity angiotensin-converting enzyme inhibition peptide sourcing from marine microalgae. The current angiotensin-converting enzyme inhibiter mainly comes from food and large macroalgae of laver and the like, and coming from bait microalgae is not reported. The structure of the obtained active peptide with a unique structure is Tyr-Met-Gly-Leu-Asp-Leu-Lys. The structure is novel and has the characteristics of being small in molecular weight and easily absorbed by a human body.

The invention relates to a saline alkali land improved special compound fertilizer for improving saline alkali lands and growing crops in the saline alkali lands, which is characterized in that the saline alkali land improved special compound fertilizer comprises 15 kilograms of devulcanized slag, 8 kilograms of bentonite, 25 kilograms of monoammonium phosphate, 27 kilograms of urea, 21 kilograms of potassium chloride or potassium sulfate and 4 kilograms of zinc sulfate as calculated by 100 kilograms of compositions; and the special compound fertilizer can provide organic nutritive compositions such as medium elements, microelements and humic acid, improve the quality of the crops, increase the reserve of soil nutrients, improve the effectiveness of nitrogen, phosphorus, potassium and the microelements, have important function on the balance of soil nutrient resources in the saline alkali lands, update and activate old organic substances, and improve the quality of humic substances, thereby comprehensively improving the soil fertility, the utilization rate of the nitrogen, and the activity of various enzymes such as invertase, protease and amylase in the soil, reducing ammonia nitration, promoting phosphorus absorption of the crops, reducing environmental pollution, contributing to environmental protection, and reducing the amount of the fertilizer, consequently saving the fertilization cost.

The invention provides a halohydrin dehalogenase encoded gene, a recombinant vector containing the gene, and a recombinant genetically engineered bacterium, and provides a novel bacterial strain, namely parvibaculum lavamentivorans ZJB 14001, of the gene. The recombinant halohydrin dehalogenase is regarded as an invertase to synthesize epoxy chloropropane by taking catalysis of 1,3-dichloro-2-propanol as an example, to prepare (S)-4-chloro-3-hydroxybutyronitrile by taking catalysis of CN-mediated (S)-epoxy chloropropane with a ring-opening reaction as an example, and to respectively prepare (S)-4-chloro-3-hydroxybutyronitrile and ethyl-(R)-4-cyano-3-hydroxybutyrate by taking catalysis of 1,3-dichloro-2-propanol and (S)-4-chloro-3-hydroxybutyronitrile as an example with CN-mediated ring opening.

The invention relates to a preparation method of a biosensor for detecting L-histidine and application thereof. Firstly, single strand DNA1 modified by biotin is fixed on magnetic beads (MBs) modified by streptavidin (SA) to obtain a compound DNA1-MBs. In addition, colloidal gold is modified by DNA2 and invertase to obtain DNA2-invertase modified colloidal gold. Then, DNA1-MBs and DNA2-invertase modified colloidal gold are mixed to obtain an L-histidine sensor. A certain amount of L-histidine is added into the prepared L-histidine sensor to react for a period of time, through magnetic separation, a supernatant is obtained, saccharose is added into the supernatant to react for a period of time and is converted to glucose by invertase, generated glucose is directly detected by a glucometer, and the content of L-histidine is detected by detecting the content of glucose. The sensor is high in sensitivity and selectivity; the glucometer is used as a detection instrument and the magnetic beads are used as carriers; the operation is simple; the detection instrument is small in size, low in price and convenient to carry.

The invention discloses a synthetic method of 11alpha hydroxy-canrenone, comprising the following steps of: heating and dissolving canrenone, propylene glycol and tween 80 to obtain a substrate; adding the substrate into a culture medium; adding an apergillus ochraceus strain and fermenting; and after the fermenting is finished, extracting and refining from a mycelium to obtain the 11alpha hydroxy-canrenone. By adopting a biosynthesis technology, novel invertase can be selected, thereby greatly reducing the cost of the 11alpha hydroxy-canrenone. The specificity of the invertase on the canrenone is improved to over 85 percent from the traditional 75 percent; the feeding concentration of the canrenone is improved to over 0.8 percent from 0.6 percent; the yield of the 11alpha hydroxy-canrenone is improved to over 60 percent from 50 percent; and main quality indexes of the 11alpha hydroxy-canrenone reach that: the content (HPLC-High Performance Liquid Chromatography) is not less than 98 percent, the content of total impurities is not more than 2.0 percent and the content of single impurity peak is not more than 1.5 percent.

A method of using high efficiency chromatograph and mass spectrum unitedly to quickly screen inhibitor of angiotonin converzyme uses hippuric acid bialkyd as substrate and uses purified rabbit lung angiotonin converzyme as source of angiotonin converzyme for carrying out quick analysis on vitality of angiotonin converzyme in human blood plasma.

The invention provides a co-production method of high-purity fructose-glucose powder by using fructo-oligosaccharide. The method comprises the following steps: (1) preparing materials; (2) transforming immobilized enzyme; (3) decoloring and filtering; (4) hybridizing; (5) performing chromatographic separation; (6) separating mother liquor; (7) performing invertase enzymolysis; (8) perfoming isomerization; (9) preparing fructose-glucose syrup; (10) drying; and (11) preparing the fructose-glucose powder, wherein fructosyl transferase which is immobilized by adopting an alumina-carrageenan-glutaraldehyde crosslinking immobilization technology is transformed, and high-purity fructo-oligosaccharide is produced by adopting a chromatographic separation technology, and separated mother liquor containing monosaccharide and cane sugar as main components is generated; the mother liquor has complex components, cannot be utilized in a general state, and is directly drained, so that resource waste and environment pollution are caused. Therefore, the cane sugar and little fructo-oligosaccharide in the separated mother liquor are subjected to invertase enzymolysis, fructose-glucose is produced by refining and isomerizing, and the fructose-glucose powder is prepared by adopting an advanced vacuum drying technology, so that the comprehensive utilization of the fructo-oligosaccharide is realized, and the considerable economical benefit is obtained.

The invention discloses an efficient agaricus bisporus culture medium prepared from crop straws and vinasse, wherein the culture medium comprises the following components: 36-44 parts by weight of wheat straw, 20-30 parts by weight of rice straw, 37-43 parts by weight of vinasse, 9-11 parts by weight of soybean cake powder, 1-2 parts by weight of cellulase, 0.8-1 part by weight of xylanase, 0.1-0.3 part by weight of Aspergillus oryzae, 0.3-0.5 part by weight of Aspergillus niger, 1.2-1.4 parts by weight of candida utilis, 1.1-1.3 parts by weight of bacillus subtilis, 1-2 parts by weight of solid lactobacillus powder, 2-3 part by weight of zeolite, and 1-2 part by weight of kieselguhr. The activation and biochemical invertase treatments are applied to the straws for preparing highly soluble straws; then mixing the straws with the fermented soybean cake for preparing highly active biochemical humic acid through high-temperature stirring reaction; therefore, the culture medium is conveniently absorbed and utilized by the agaricus bisporus, so as to accelerate the metastasis of the agaricus bisporus. The conditions of generating intermediate products of the biochemical humic acid are created by loading the zeolite and the kieselguhr to the biochemical invertase and the zymophyte.

Disclosed is an apparatus and method for continuously converting sucrose to β-D-glucose. The method comprises a three stage enzymatic reactor in which an aqueous solution of sucrose is first converted into a solution of fructose and α-D-glucose by passing it through a porous, packed column containing an inert media on which invertase is immobilized. This solution is then sent through a second packed column containing glucose isomerase and finally a third packed column containing mutarotase. Solution temperature and pH are adjusted to maximize glucose output.

The invention discloses a method for preparing low blood sugar starch by enzymatic modification, which belongs to the technical field of deep processing of starch. The method biologically modifies and recombines a starch molecular chain fine structure after commercialized massive starch products of corn starch, wheat starch, rice starch, potato starch, cassava starch, sweet potato starch and the like are pasted by using the hydrolysis catalyzing technology of sucrase so as to obtain low blood sugar starch derivatives rich in slow-digestion starch and resistant starch; and the content ratios of the slow-digestion starch and the resistant starch in the low blood sugar starch product are improved by 15 percent and 10 percent respectively compared with the primary starch. The application and the development of the product relate to a plurality of fields of food, medicament and the like; and the low blood sugar starch is not only the medicinal food for diabetes, obesity, hypertension and cardiovascular disease patients, but also can be used as special food for athletes slowly releasing continuous energy or ideal health-care food for healthy people.

The present invention relates to a novel compound of the formula I:

and/or all stereoisomeric forms of the compound of the formula I and/or mixtures of these forms in any ratio, and/or a physiologically tolerated salt of the compound of the formula I, in which R1 to R5 and V1, V2 have the meanings stated in the claims and specification. The inventive compounds are suitable as inhibitors of metalloproteases, especially of ADAMTS proteases and TNF-α converting enzyme (TACE), and for the treatment of disorders such as but not limited to osteoarthrosis and rheumatoid arthritis.

A process for preparing Angiotensin Converting Enzyme (ACE) inhibitors from glycinin of soy flour and its use as ACE inhibitors.