75 results about "Invertase" patented technology

The invention relates to the technical field of biological catalysis, and discloses an L379A mutant enzyme for preparing rebaudioside I. The enzyme is obtained by the following mutations generated by UGT76G1: leucine of the 379th amino acid sequence is mutated into alanine; the enzyme can be applied to RA in-vitro conversion preparation of RI with higher utilization value, the conversion rate is higher than 50%, and the enzyme activity is remarkably improved by 7 times compared with the original enzyme catalytic conversion enzyme activity. The UGT76G1 mutant disclosed by the invention has the advantages that (1) the blank is filled, and a special enzyme catalyst capable of realizing efficient in-vitro synthesis of rebaudioside I (RI) is provided for the first time; 2) efficiency jump: the catalytic conversion rate is greatly increased from original about 7% to more than 50% (increase gt; and 3) stability and reliability: in the cross-scale reaction of 10mL to 5L, the catalyst has stable catalytic performance, shows excellent industrial application potential, and is suitable for popularization and application.

The invention provides a preparation method and application of a bacterium-enzyme-magnetic nano-cluster composite detoxicating agent, the detoxicating agent takes a Fe3O4-HAP nano-cluster as a magnetic core, the surface of the detoxicating agent is subjected to functional modification, a composite flora composed of bacillus amyloliquefaciens, lactobacillus plantarum and saccharomyces cerevisiae is loaded to form sclerotia, a chitosan shell layer is constructed on the outermost layer, and the bacterium-enzyme-magnetic nano-cluster composite detoxicating agent is prepared. And a compound enzyme consisting of aflatoxin Bdegrading enzyme, zearalenone hydrolase and deoxynivalenol invertase is immobilized. The carrier can efficiently and synchronously degrade aflatoxin, vomitoxin and zearalenone, and can realize rapid separation and recovery through an external magnetic field.

The invention provides an application of an OsINV2 gene in regulation and control of rice cold stress resistance. A nucleotide sequence of the OsINV2 is shown as SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 or SEQ ID NO: 4. By revealing the key regulation and control effect of the vacuole sucrose invertase gene OsINV2 in rice low-temperature stress response, the molecular mechanism that the gene affects the cold resistance of plants by regulating the sugar metabolism balance in vacuoles is illustrated for the first time. The expression of the OsINV2 is regulated in a targeted manner through molecular marker-assisted breeding or a gene editing technology, so that the low-temperature survival rate in the seedling stage and the cold resistance in the booting stage of the rice can be remarkably improved, glumous flower degeneration and pollen abortion caused by low temperature are reduced, and meanwhile, the limitation of the cold resistance of varieties on geographical distribution is broken through; a theoretical support and a technical path are provided for breeding and adaptive popularization of cold-resistant rice varieties.

The invention relates to a multi-component ginseng extract containing ginseng polypeptide and a preparation method of the multi-component ginseng extract. The preparation method comprises the following steps: S1, preparing ginseng powder from a ginseng raw material; mixing the ginseng powder with an acidic buffer solution, and carrying out presoaking treatment and homogenate dissolution promoting treatment to obtain ginseng primary pulp; s2, mixing the wall-breaking enzyme system with the ginseng primary pulp, and performing acid wall-breaking treatment to obtain wall-broken primary pulp; mixing an invertase system with the wall-broken primary pulp, and carrying out enzymolysis synchronous glucoside conversion treatment to obtain a crude extract; s3, filtering and separating the crude extracting solution to obtain an extract containing the ginseng polypeptide; the pH values of the ginseng primary pulp, the wall-broken primary pulp and the crude extract are in a range of 4.0-6.0; the wall breaking enzyme system comprises pectinase and cellulase; the invertase system comprises beta-glucosidase and acid protease. The technical problems that in the prior art, a ginseng extract is complex in extraction process, multiple active ingredients are difficult to efficiently extract at the same time, the product quality is low, and the biological activity is poor are solved.

The invention relates to the field of synthetic biology, metabolic engineering and fermentation engineering, and discloses a genetically engineered bacterium and application thereof in production of alpha-ketoglutaric acid.The genetically engineered bacterium comprises the steps that the genetically engineered strain with escherichia coli BW25113 or corynebacterium glutamicum ATCC13032 as a host is constructed; overexpression type isocitrate dehydrogenase genes are integrated through chromosomes, alpha-ketoglutarate dehydrogenase complex genes are knocked out, NADPH invertase is overexpressed through plasmids, and exogenous AKG transporter protein is introduced, so that metabolic flux directional regulation and control and product efflux enhancement are realized; meanwhile, a staged fermentation process is adopted and comprises dynamic gradient adjustment of inorganic phosphorus concentration and two-stage pH control. According to the method, the AKG yield of 78.2 g / L can be obtained under the 5L tank scale, the sugar acid conversion rate reaches 0.68, and the method has the advantages of being high in yield, low in byproduct and controllable in cost. The obtained AKG is suitable for a plurality of fields such as medical intermediates, functional foods and cell culture additives, and has wide industrial application prospects.

The application discloses a polypeptide-based anti-fouling electrochemical sensor and a construction method and application thereof. In the application, cysteine is used as an anchoring end, an alpha-helix structure is formed by inserting polyproline to give a supporting effect to the whole peptide, then angiotensin converting enzyme inhibitory peptide (KSRE) and alpha-glucosidase inhibitory peptide (EKDER) are used as branches to prepare the anti-fouling polypeptide. The anti-fouling polypeptide has a better anti-fouling effect than other anti-fouling polypeptides, and the working electrode is modified by the anti-fouling polypeptide to prepare an anti-fouling electrochemical aptamer sensor for detecting AFB1, so that the anti-pollutant interference effect of the sensor can be greatly improved, the accuracy and sensitivity of AFB1 detection can be improved, and the anti-fouling electrochemical aptamer sensor has a wide application prospect.

The present invention discloses a method for preparing milk-derived angiotensin-converting enzyme inhibitory peptides by fermentation with composite strains, which belongs to the fields of microbial technology and fermentation. The ratio of whey protein hydrolysate with a molecular weight below 1000 Da and the angiotensin-converting enzyme inhibition rate in the milk-based fermentation broth are used as evaluation indicators. The composite strain Lactobacillus helveticus ( Lactobacillus helveticus , CICC: 20289), composite strain Pediococcus dilactici ( Pediococcus acidilactici , CICC:20719) were rescreened to obtain strains with excellent whey protein hydrolysis performance and high angiotensin-converting enzyme inhibitory peptide production capacity. The two composite strains obtained from the rescreening were enriched and cultured separately, and then inoculated into skim milk powder culture medium at a specific compound ratio. Through composite fermentation, the angiotensin-converting enzyme inhibitory peptide content in the milk-based fermentation product was increased. The process of this invention is rationally designed and highly operable. It provides a reference for further increasing the content of small molecule functional peptides in fermented dairy products, efficiently preparing milk-derived angiotensin-converting enzyme inhibitory peptides, and improving the nutritional value of dairy products, and has great application prospects.

The present application relates to the field of agricultural biotechnology, and particularly relates to a molecular marker capable of identifying allelic variations of a soluble acid invertase gene AcoInv-1 in pineapple fruits and application thereof. The molecular marker is amplified by using the following primers: forward primer 5-CTGCGAAATGAACTAGTCAACTC-3 and reverse primer 5-TAGAGAACCGTTCTTGG AATGG-3. The primers can identify allelic variations of the soluble acid invertase gene AcoInv-1 in pineapple fruits, i.e. AcoInv-1a and AcoInv-1b. It is found through research that the two allelic variations are extremely significantly related to the total sugar content of the pineapple fruits. It is proved through verification that the pineapple material of the AcoInv-1a type is related to high sugar content, and the pineapple material of the AcoInv-1a and AcoInv-1b types is related to low sugar content. The application of the molecular marker obtained by the present application in selecting pineapple varieties with high sugar content potential will help to accelerate the breeding selection cycle.

The present application relates to the field of plant genetic breeding, and particularly relates to a kind of evaluation method of asparagus yield. Including the following steps: S1, obtaining young plant period asparagus;S2, measures i variety of young plant period asparagus following parameters: photosynthetic rate A, ribulose-1,5-bisphosphate carboxylase / oxygenase activity B, leaf dry weight per unit area C, acid invertase activity D and root crown ratio E;The measurement sample of the acid invertase activity D is the tender stem of young plant period asparagus at 10-30cm from top;S3, according to the formula, the evaluation value is calculated, and the evaluation value is used to evaluate the yield of asparagus. The evaluation value obtained by using the above measurement index and calculation formula, and the significance level obtained by SPSS software is used to evaluate the yield of asparagus. The yield potential of asparagus can be comprehensively evaluated from the genetic point of view in a short time. Using the evaluation method of the present application, high-yield asparagus varieties can be selected in a short time without tracking for many years.

The present invention discloses a highly active ginseng fermented product and its application. Ginseng is fermented using a composite strain composed of independently screened plant lactobacillus Byf‑r‑01 and grape juice spore Hansen yeast Byf‑j‑01. The composite strain metabolic enzyme system is highly complementary, and the prototype ginsenosides are efficiently converted into rare ginsenosides in a collaborative manner, while simultaneously improving the content of polyphenols, flavonoids, alkaloids and volatile oils. The present invention adds chitosan-polyaspartic acid complex to neutralize lactic acid and maintain pH stability. At the same time, it promotes the proliferation of composite strains as a nitrogen source, significantly improves strain biomass and the activity of saponin-related converting enzymes, and promotes the conversion of ginsenosides. The highly active ginseng fermented product prepared by the present invention has both strong antioxidant and anti-fatigue functions. It has been confirmed by animal experiments that it can prolong the exhaustive swimming time of mice, reduce blood urea nitrogen levels, increase liver / muscle glycogen reserves, and significantly alleviate lactic acid accumulation after exercise, providing a new path for the development of high value-added functional products for ginseng.

The invention discloses a Thomasclavia ramosa CMLF06 strain, saponin converting enzyme and application of the Thomasclavia ramosa CMLF06 strain and the saponin converting enzyme, the Thomasclavia ramosa CMLF06 strain is preserved in China General Microbiological Culture Collection Center, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is January 25, 2024, and the preservation number is CGMCC NO. 41093. The invention further discloses a method for preparing the saponin converting enzyme. The Thhomasclavia ramosa CMLF06 strain can convert ginsenoside to generate rare ginsenoside and sapogenin, the saponin converting enzyme GE001065 can convert ginsenoside and notoginsenoside to generate various rare ginsenoside in the presence of ethanol, the activity and stability of the enzyme GE001065 in an ethanol solution and a methanol solution are remarkably improved, the enzyme GE001065 shows an activation effect in various alcohols, and the enzyme GE001065 can be used for preparing the rare ginsenoside. The method can be used in the fields of medicine and functional food.

The invention relates to the field of protein, in particular to a milk-derived peptide APPSPIGVF with antihypertensive activity as well as a preparation method and application of the milk-derived peptide APPSPIGVF, and the amino acid sequence of the milk-derived peptide APPSPIGVF is Ala-Pro-Pro-Ser-Pro-Ile-Gly-Val-Phe. An angiotensin converting enzyme activity inhibition experiment, an alpha-amylase activity inhibition experiment and a dipeptidyl peptidase-4 activity inhibition experiment prove that the milk-derived peptide APPSPIGVF has very good functions of resisting hypertension and reducing blood sugar. The milk-derived peptide APPSPIGVF disclosed by the invention has an anti-hypertension capability, and can be used for remarkably inhibiting the activity of alpha-amylase and dipeptidyl peptidase-4, improving the capability of resisting chronic diseases such as hypertension and hyperglycemia of an organism, reducing the morbidity of the organism and improving the quality of life; the method has very important significance for developing food, health care products and medicines with the functions of resisting hypertension and reducing blood sugar.

The invention discloses a biosynthesis process of creatine monohydrate, and belongs to the technical field of bioengineering. The invention discloses a creatine monohydrate biosynthesis method, which is characterized in that sarcosine invertase is utilized to catalyze chloroacetic acid and monomethylamine to generate N-alkylation reaction so as to prepare sarcosine, and then creatine monohydrate is generated under the catalysis of the creatine invertase, the process is mild in reaction condition, the temperature is 25-37 DEG C, the pH is 6.0-8.0, the energy consumption is lower, the conversion efficiency is high, the environment is protected, and the economical efficiency is high. The invention provides a new synthesis method for sustainable synthesis of creatine monohydrate, and provides a new idea for industrial production of creatine monohydrate.

The invention discloses a preparation method and application of an edible grass leaf source active peptide with ACE (angiotensin converting enzyme) inhibitory activity, and belongs to the technical field of biological active peptides. The preparation method comprises the following steps: firstly, extracting azolla filliculoidas protein from azolla filliculoidas powder by adopting an ultrasonic-assisted alkali-solution and acid-isolation method; dissolving a certain amount of rumex hanus powder in distilled water, adjusting the pH value of the solution, carrying out water bath preheating, and adding neutral protease for enzymolysis; and carrying out ultrafiltration and DEAE Sepharose FF anion gel column separation on the obtained enzymolysis product to obtain the azolla filliculoidas source active peptide with efficient angiotensin converting enzyme inhibitory activity. The preparation method is simple in process, mild in condition and easy to popularize, the obtained active peptide has wide application prospects in development of functional food, health care products and antihypertensive drugs, and effective utilization and value improvement of azolla filliculoidas protein resources are facilitated.

The present disclosure provides pharmaceutical compositions comprising fibronectin based scaffold domain proteins that bind, for example, proprotein convertase subtilisin kexin-9 (PCSK9).

This invention discloses a strain of *Lactobacillus casei* that produces thermostable invertase and its applications. This strain was deposited on August 14, 2023, at the China Center for Type Culture Collection (CCTCC), Wuhan, Hubei Province, China, with accession number CCTCCNO: M20231470. The *Lactobacillus casei* provided by this invention is easy to culture and can produce high levels of thermostable invertase. Its probiotic powder can rapidly degrade sucrose and prevent sucrose intolerance. The metabiotic prepared from this strain can also effectively degrade sucrose and prevent sucrose intolerance.

This invention belongs to the field of biotechnology, specifically relating to a multifunctional bioactive peptide based on millet protein and its preparation method. The amino acid sequence of the bioactive peptide is shown in SEQ ID NO: 1. The bioactive peptide of this invention can activate alcohol dehydrogenase activity and inhibit xanthine oxidase and angiotensin-converting enzyme activity. It has the characteristics of small molecular weight and easy absorption. Furthermore, bioinformatics analysis predicts that it has advantages such as non-toxicity, non-carcinogenicity, high hydrophilicity, and long half-life. It can be used to develop drugs with therapeutic effects on diseases such as hangover relief, uric acid reduction, and blood pressure reduction, and has broad application prospects.

The invention discloses a multifunctional bioactive peptide from highland barley and application of the multifunctional bioactive peptide. The bioactive peptide has an amino acid sequence as shown in SEQ ID NO: 1. The multifunctional bioactive peptide disclosed by the invention has the activity of inhibiting angiotensin converting enzyme, the activity of inhibiting dipeptidyl peptidase-4, the antioxidant activity and the anti-inflammatory activity, can be used for developing polypeptide medicines or functional foods with a disease treatment effect, and has a good application prospect.

The invention provides a reagent and a kit for detecting ADP (adenosine diphosphate), a detection method of ADP and application of the detection method, and belongs to the technical field of ADP detection. The reagent for detecting the ADP comprises ATP sulfating enzyme, pyrophosphatase, an enzyme inhibitor, invertase for catalyzing the ADP into ATP, a substrate of the invertase, bioluminescent enzyme and a substrate of the bioluminescent enzyme, wherein the enzyme inhibitor inhibits the activity of the ATP sulfating enzyme and the pyrophosphatase. According to the present invention, the ADP detection accuracy and the ADP detection sensitivity are high, the fluorescence signal is stable, and the reagent can be used for detecting the enzyme activity of phosphotransferase and / or ATP hydrolase.

Disclosed are, inter alia, combinations comprising a first antisense (e.g., first double stranded RNAi (dsRNAi)) agent for inhibiting expression of proprotein convertase subtilisin kexin 9 (PCSK9), for example, human PCSK9, and a second antisense agent (e.g., second dsRNAi agent) for inhibiting expression of lipoprotein (a) (Lp(a)); pharmaceutical compositions including the same, and methods of treatment using the same.

This invention provides a sea buckthorn seed protein peptide, its preparation method, and its application, relating to the field of plant protein peptide extraction technology. The preparation method includes: pulverizing defatted sea buckthorn seed residue, performing ultrasonic-assisted extraction, adjusting the pH value to precipitate, and centrifuging to obtain a protein extract; adding a complex protease composed of alkaline protease, flavor protease, and neutral protease in a specific enzyme activity ratio for targeted enzymatic hydrolysis; purifying the hydrolysate sequentially through a 3kDa ultrafiltration membrane and a 1kDa nanofiltration membrane, collecting the permeate; adding an encapsulating agent and spray drying to obtain the final product. The sea buckthorn seed protein peptide prepared by this method has a molecular weight of less than 3kDa (≥85%), a purity of ≥90%, and exhibits excellent DPPH free radical scavenging ability and angiotensin-converting enzyme inhibitory activity. This invention achieves efficient extraction of sea buckthorn seed protein and targeted enrichment of active peptides. The product can be used as a functional food, health product, or pharmaceutical raw material for antioxidation and blood pressure reduction.

The invention belongs to the technical field of development of plant-derived bioactive peptides, and discloses a preparation method and application of a moringa seed antihypertensive polypeptide. Moringa seed polypeptide with the activity of inhibiting angiotensin converting enzyme (ACE) is separated and purified from moringa seed crude protein, nine moringa seed ACE inhibitory peptides with brand-new sequences and the effect of reducing blood pressure are finally obtained through structural identification, activity screening and solid-phase synthesis, sequence modification is conducted on polypeptide PLVRRAIY with the highest activity, and the trypsin enzymolysis resistance of the polypeptide PLVRRAIY is improved. Molecular level and animal experiments show that both the original peptide PLVRRAIY and the modified peptide PPVQQAIY have significant ACE inhibitory activity and antihypertensive effect, the trypsin degradation resistance of the modified peptide in the gastrointestinal tract environment is enhanced, the stability is significantly improved, and a new resource is provided for development of natural antihypertensive polypeptide drugs.

This invention belongs to the field of resource utilization technology of cooking wine residue, and discloses an ACE-inhibiting peptide derived from cooking wine residue. To improve the economic value of cooking wine residue and develop an oligopeptide with antihypertensive effects, this invention uses cooking wine residue as raw material and obtains an antihypertensive peptide monomer through various separation and purification methods such as water extraction, membrane filtration, and high-performance liquid chromatography. The amino acid sequence is WCYND, and the molecular weight is 699.73 Da. It exhibits good activity, can be artificially synthesized, and is safe and non-toxic. Its ACE-inhibiting activity has been measured, and this antihypertensive oligopeptide has an inhibitory effect on angiotensin-converting enzyme in vitro, which can be applied in the field of antihypertensive treatment and is beneficial for the high-value comprehensive utilization of cooking wine residue.

To provide an injection device for subcutaneous administration, for preventing cardiovascular events in a human subject.SOLUTION: The present invention provides an injection device for subcutaneous administration, comprising a fixed dose of 275 mg to 325 mg of an interfering ribonucleic acid (RNAi) agent. The device comprises a double-stranded ribonucleic acid comprising a sense strand and an antisense strand forming a double-stranded region, where the antisense strand comprises the nucleotide sequence of 5'-asCfsaAfAfAfgCfaAfaAfcAfgGfuCfuagsasa-3' (SEQ ID NO: 3), and the sense strand comprises the nucleotide sequence of 5'-csusagacCfuGfudTuugcuuuugu-3' (SEQ ID NO: 4), where a, g, c and u are 2'-O-methyl (2'-OMe) A, G, C, or U; Af, Gf, Cf or Uf are 2'-fluoro A, G, C or U; dT is 2'-deoxythymidine; and s is a phosphorothioate linkage.SELECTED DRAWING: None

Compositions and methods relating to ApoA-1 fusion polypeptides are disclosed. The fusion polypeptides include a first polypeptide segment corresponding to an ApoA-1 polypeptide or ApoA-1 mimetic, and may also include a dimerizing domain such as, e.g., an Fc region, which is typically linked carboxyl-terminal to the first polypeptide segment via a flexible linker. In some embodiments, the fusion polypeptide further includes a second polypeptide segment located carboxyl-terminal to the first polypeptide segment and which confers a second biological activity (e.g., an RNase, paraoxonase, platelet-activating factor acetylhydrolase, cholesterol ester transfer protein, lecithin-cholesterol acyltransferase, polypeptide that specifically binds to proprotein convertase subtilisin / kexin type 9, or polypeptide that specifically binds to amyloid beta). Also disclosed are dimeric proteins comprising first and second ApoA-1 fusion polypeptides as disclosed herein. The fusion polypeptides and dimeric proteins are useful in methods for therapy.

The invention relates to the field of immunosensors, and discloses a preparation method of a double-signal intelligent immunosensor and application of the double-signal intelligent immunosensor in silk fibroin detection. The preparation method comprises the following steps: (1) preparing the INV / HRP-ZIF-90 nanoparticles; (2) preparing an antibody functionalized magnetic bead probe solution; (3) preparing an Ab2-INV / HRP (at) ZIF-90 solution; and (4) preparing the double-signal intelligent immunosensor. According to the double-signal immunosensor, on the basis of an immunomagnetic bead ZIF-90 sandwich structure, ATP is used for triggering release of double enzymes (sucrose invertase and horse radish peroxidase), and a double-mode detection means of glucometer signal conversion and TMB color development-Image J analysis is combined, so that qualitative and semi-quantitative detection of a target antigen in silk can be realized, double signals can be mutually verified, and the detection sensitivity is high. And the detection accuracy is improved. The double-signal immunosensor not only has strong response specificity, but also has good portability, and can meet the requirements of on-site rapid detection and result verification of silk cultural relics.

This invention belongs to the field of food additive content detection and discloses a dual-detection zone test strip for analyzing glucose and sucrose content. The test strip uses Au@Fe, a cluster-polyphenol-metal coordination network nanozyme with high peroxidase activity, as the signal conversion unit. It constructs a multi-enzyme cascade catalytic process by coupling glucose oxidase (GOx) and invertase (IVt). The RGB color channel signals of the test strip are acquired using smartphone image recognition. A concentration signal correlation between glucose and sucrose is established by unifying the dual-detection zone signal benchmark, ultimately achieving highly sensitive and simultaneous accurate detection of both components in a glucose and sucrose coexisting system. With its advantages of portability, visualization, ease of operation, high sensitivity, and accurate results, this test strip can meet the needs of beverage sugar content detection in daily life, providing a valuable detection tool for controlling daily sugar intake to prevent diseases such as diabetes and obesity.

Disclosed herein are methods of producing food products or food precursors. The methods can include (a) providing a food product / precursor comprising at least water and sucrose, and (b) contacting the food product / precursor with at least one glucosyltransferase (GTF) and, in some aspects, a second enzyme using sucrose as a substrate, such as an invertase. The one or more GTFs may be selected from the group consisting of (i) a GTF that synthesizes alpha-1, 6-glucan, and / or (ii) a GTF that synthesizes alpha-1, 3-glucan. Such enzymatic treatment typically results in the production of alpha-glucan in the food product / precursor. Treatment of a food product / precursor with a second enzyme using sucrose as a substrate can alter the effects of GTF treatment on the food product / precursor, such as texture formation, sugar composition, and / or sweetness. Food products and food precursors produced by such methods are also disclosed.

The invention relates to a bifunctional peptide from cyclina sinensis, and belongs to the technical field of polypeptides. The amino acid sequence of the bifunctional peptide disclosed by the invention is His-Tyr-Pro-Gly-Gln-Pro-Tyr, and the amino acid sequence of the bifunctional peptide disclosed by the invention is as shown in the specification. The invention relates to an oligopeptide with alpha-glucosidase inhibitory activity and ACE (angiotensin converting enzyme) inhibitory activity. The invention also discloses a preparation method of the bifunctional peptide. The bifunctional peptide can also be synthesized by adopting a peptide synthesis method or a synthesis and modification method. The bifunctional peptide provided by the invention takes protein from marine cyclina sinensis as a starting point, is cut into active peptide with a specific sequence structure through control of cutting conditions of protease, has remarkable alpha-glucosidase inhibitory activity and angiotensin converting enzyme (ACE) inhibitory activity, has ICvalues of 0.4662 mg / mL and 0.09976 mg / mL respectively, and can be used for preparing the marine cyclina sinensis bifunctional peptide. Therefore, the tea has double physiological functions of reducing blood sugar and blood pressure. The bifunctional peptide provided by the invention can be used for preparing medicines or health-care foods for reducing blood sugar and / or blood pressure, has a clear sequence structure and dual biological activity, and provides a new way for developing multifunctional ocean-derived peptide products.

The present application provides a kind of yeast peptide and its preparation method and application.The yeast peptide provided by the present application contains the following sequence peptide segment: phenylalanine-valine-alanine-leucine-proline, valine-leucine-isoleucine-leucine-lysine and alanine-glycine-leucine-threonine-leucine.The present application provides angiotensin converting enzyme (ACE) inhibitory activity and in vivo blood pressure lowering activity.