Co-production method of high-purity fructose-glucose powder by using fructo-oligosaccharide

A high-purity fructooligosaccharide technology, applied in fermentation and other directions, can solve the problems of large amount of resin, low recycling rate, low recovery rate of enzyme activity, etc., and achieve the effect of simplifying production process, short production cycle and reducing production cost.

Inactive Publication Date: 2012-10-03
BAOLINGBAO BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The enzyme activity recovery rate of the immobilized enzyme is low, the recycling

Method used

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  • Co-production method of high-purity fructose-glucose powder by using fructo-oligosaccharide
  • Co-production method of high-purity fructose-glucose powder by using fructo-oligosaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A method for high-purity fructo-oligosaccharide co-production fructose powder, it comprises the steps:

[0038] (1) Immobilized fructosyltransferase: the volume ratio of alumina and enzyme solution is 1:2.5, at pH 6.5, in a 55°C water bath, and oscillating at a speed of 150rpm for 2-4h, add glutaraldehyde solution, cross-linking agent The final concentration of glutaraldehyde is 0.3%. After cross-linking at 4-10°C and 90rpm for 4-6h under slow shaking, filter and wash with pH6.0-7.0 phosphate buffer to wash glutaraldehyde residue and unfixed The free enzyme until the eluate has no enzyme activity, that is, the first immobilized fructosyltransferase is obtained. Add the primary immobilized fructosyltransferase to 20g / L carrageenan solution at a weight percentage of 15%, then embed it with carrageenan, dry it in a 28°C incubator for 5 hours, cut it into small pieces, and place it in 0.2%-0.4% The glutaraldehyde solution was hardened for 6-8 hours, and the glutaraldehyde ...

Embodiment 2

[0050] A method for high-purity fructo-oligosaccharide co-production fructose powder, it comprises the steps:

[0051] (1) Immobilized fructosyltransferase: the volume ratio of alumina and enzyme solution is 1:3, at pH 7.0, in a 55°C water bath, and oscillating at a speed of 200rpm for 2-4h, add glutaraldehyde solution, cross-linking agent The final concentration of glutaraldehyde is 0.4%. After cross-linking at 4-10°C and 100rpm for 4-6h under slow shaking, filter and wash with phosphate buffer solution of pH 6.0-7.0 to wash glutaraldehyde residue and unfixed The free enzyme until the eluate has no enzyme activity, that is, the first immobilized fructosyltransferase is obtained. Add the primary immobilized fructosyltransferase to 20g / L carrageenan solution at a weight percentage of 20%, then embed it with carrageenan, dry it in a 28°C incubator for 5 hours, cut it into small pieces, and place it in 0.2%-0.4% The glutaraldehyde solution was hardened for 6-8 hours, and the glu...

Embodiment 3

[0062] A method for high-purity fructo-oligosaccharide co-production fructose powder, it comprises the steps:

[0063] (1) Immobilized fructosyltransferase: the volume ratio of alumina and enzyme solution is 1:2.5, at pH 6.0, in a 55°C water bath, and oscillating at a speed of 100rpm for 2-4h, add glutaraldehyde solution, cross-linking agent The final concentration of glutaraldehyde is 0.2%. After cross-linking at 4-10°C and 90rpm for 4-6h under slow shaking, filter and wash with pH 7.0 phosphate buffer to wash away glutaraldehyde residue and unfixed free Enzyme, until the eluate has no enzyme activity, the first immobilized fructosyltransferase is obtained.

[0064] (2) The production process parameters of high-purity fructo-oligosaccharide and fructose powder are carried out according to Example 1. The immobilized fructosyltransferase obtained according to the above method is used in the production of high-purity fructo-oligosaccharides and fructoglucose powder with an enzy...

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Abstract

The invention provides a co-production method of high-purity fructose-glucose powder by using fructo-oligosaccharide. The method comprises the following steps: (1) preparing materials; (2) transforming immobilized enzyme; (3) decoloring and filtering; (4) hybridizing; (5) performing chromatographic separation; (6) separating mother liquor; (7) performing invertase enzymolysis; (8) perfoming isomerization; (9) preparing fructose-glucose syrup; (10) drying; and (11) preparing the fructose-glucose powder, wherein fructosyl transferase which is immobilized by adopting an alumina-carrageenan-glutaraldehyde crosslinking immobilization technology is transformed, and high-purity fructo-oligosaccharide is produced by adopting a chromatographic separation technology, and separated mother liquor containing monosaccharide and cane sugar as main components is generated; the mother liquor has complex components, cannot be utilized in a general state, and is directly drained, so that resource waste and environment pollution are caused. Therefore, the cane sugar and little fructo-oligosaccharide in the separated mother liquor are subjected to invertase enzymolysis, fructose-glucose is produced by refining and isomerizing, and the fructose-glucose powder is prepared by adopting an advanced vacuum drying technology, so that the comprehensive utilization of the fructo-oligosaccharide is realized, and the considerable economical benefit is obtained.

Description

technical field [0001] The invention relates to a new technology for high-purity fructo-oligosaccharide co-production fructose powder. The high-purity fructo-oligosaccharide is prepared by immobilized fructosyltransferase conversion, refining and chromatographic separation technology, and simultaneously produces fructo-oligosaccharide separation mother liquor; and comprehensive utilization of the separated mother liquor to realize joint production of high-purity fructo-oligosaccharide and fructose powder, belonging to the field of sugar preparation. Background technique [0002] Due to its unique structure, fructo-oligosaccharides are not digested by gastric acid and enzymes in the digestive tract, and can go directly to the large intestine, where they are rapidly and selectively absorbed by the beneficial flora Bifidobacteria, causing the Bifidobacteria to proliferate rapidly and produce short-chain fatty acids. Make the pH of the intestinal tract more acidic, inhibit the g...

Claims

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Application Information

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IPC IPC(8): C12P19/20C12P19/18
Inventor 刘宗利王乃强王彩梅杨杰王明珠李方华
Owner BAOLINGBAO BIOLOGY
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