1882 results about "Glutaraldehyde" patented technology

The invention discloses a method of treating a patient for pleural effusion comprising percutaneously delivering an adhesive material to a pleural space of the patient. Suitable adhesive materials for performing any of the embodiments of the methods of the invention can be selected from the group consisting of hydrogels, collagen, poly(lactic acid), poly(glycolide), cyanoacrylates, glutaraldehyde, PEG, protein, and polysaccharide and derivatives thereof. The invention also discloses a pleural effusion treatment apparatus comprising an adhesive material adapted to adhere pleural membranes defining a pleural space and a pleural space access member adapted to deliver the adhesive material to the pleural space.

Methods for decellularizing mammalian tissue for use in transplantation and tissue engineering. The invention includes methods for simultaneous application of an ionic detergent and a nonionic detergent for a long time period, which may exceed five days. One method utilizes SDS as the ionic detergent and Triton-X 100 as the nonionic detergent. A long rinse step follows, which may also exceed five days in length. This long duration, simultaneous extraction with two detergents produced tissue showing stress-strain curves and DSC data similar to that of fresh, unprocessed tissue. The processed tissue is largely devoid of cells, has the underlying structure essentially intact, and also shows a significantly improved inflammatory response relative to fresh tissue, even without glutaraldehyde fixation. Significantly reduced in situ calcification has also been demonstrated relative to glutaraldehyde fixed tissue. Applicants believe the ionic and non-ionic detergents may act synergistically to bind protein to the ionic detergent and may remove an ionic detergent-protein complex from the tissue using the non-ionic detergent. The present methods find one exemplary use in decellularizing porcine heart valve leaflet and wall tissue for use in transplantation.

A method of treating a biological tissue including contacting the biological tissue with an aqueous sterilizing solution, and maintaining the aqueous sterilizing solution at a temperature of about 50° C. for a time period of about 1 to 2 days. The method of treating a biological tissue may be utilized as a terminal sterilization step in a method for fixation of biological tissues, and bioprosthetic devices may be prepared by such fixation method. The fixation method may include the steps of A) fixing the tissue, B) treating the tissue with a mixture of i) a denaturant, ii) a surfactant and iii) a crosslinking agent, C) fabricating or forming the bioprosthesis (e.g., forming the tissue and attaching any non-biological components thereto) and D) subjecting the bioprosthesis to the terminal sterilization method. The aqueous sterilizing solution may be glutaraldehyde of about 0.625 weight percent buffered to a pH of about 7.4.

The invention relates to a method for preparing ethylenediamine modified chitosan composite magnetic microspheres and application thereof. The method specifically comprises the following steps of: mixing acidic aqueous solution of chitosan and a water-based magnetofluid first, then crosslinking by using glutaraldehyde, then adjusting the pH value to form a gelatinous precipitate, then modifying by using ethylenediamine and epichlorohydrin, and washing and drying to obtain a final product. The product has good absorption effect on radionuclide aranium and metal ions such as heavy metal Pb, Cr and the like; the removal rate reaches over 95 percent when the initial concentration of various ions is within the range of 200mg/L; and the product has quick absorption rate and good regeneration performance. The ethylenediamine modified chitosan composite magnetic microspheres can be used for metal recovery and pollution remediation in mines, radioactive waste water, smelteries, electronics factories and waste water of electroplating factories.

The invention relates to a preparation method of a chitosan compound flocculant which can effectively remove organic pollutants in water, can adsorb heavy metal ions such as nickel, chromium and lead in water and has high heavy metal ion adsorption capacity and high adsorption efficiency and a chitosan compound flocculant product thereof. The chitosan compound flocculant product is prepared by adopting chitosan as a raw material, glutaraldehyde as a crosslinking agent and washed and acid pickled attapulgite as a modifier. The preparation method of the compound flocculant capable of adsorbing heavy metal ions has the characteristics of simple technology, low cost, no special equipment and easy industrial implementation, the compound flocculant reduces dosage of chiston, and cost is reduced.

The invention relates to a preparation method of a protein magnetic imprinting nano sphere, in particular to a preparation method of a protein magnetic imprinting nano sphere which takes one of hemoglobin, muramidase or serum protein as template molecules; the preparation method includes the following steps: synthesizing magnetic nano particles which are Fe3O4 nano particles covered with silicon dioxide on the surfaces; carrying out silanization to the amino-groups on the surface of the magnetic sphere; using glutaric dialdehyde to connect protein; using silylation agent to fix the space structure of template protein; alkaline eluting the protein on the surface of the magnetic sphere to form imprinting sites. The hemoglobin, muramidase or serum protein magnetic nano imprinting polymer sphere prepared by the invention combines the precise and specific identification property of molecular imprinting and the quick separation property of the magnetic nano sphere under the action of an external magnetic field, integrates the advantages of molecular imprinting and the magnetic nano sphere, avoids complex centrifugation operation, plays vital roles in the separation of cells, protein and nucleic acid, the detection of biomolecules, tumor diagnosis and medicine targeting therapy and has promising application prospect in the field of isolation analysis.

The invention relates to a polyvinyl alcohol radical composite coating fresh keeping packaging material nano TiO2 functional modification method, which comprises the following steps: adding nano TiO2 and SiO2 into a polyvinyl alcohol aqueous solution and stirring; carrying out ultrasonic mixing on a mixed liquor; placing the mixed solution in a reaction kettle after ultrasonic; heating, stirring and reacting at constant temperature; adding stearic acid-alcohol mixed solution and glutaraldehyde with the same temperature and stirring; and carrying out cross-linking reaction at a constant temperature; stirring and cooling to a room temperature after reaction, so as to prepare into a nano composite coating new material. With the adoption of the polyvinyl alcohol radical composite coating fresh keeping packaging material nano TiO2 functional modification method, the nano TiO2 is adopted to modify a polyvinyl alcohol radical composite coating fresh keeping packaging material; the new material film formation moisture permeability is obviously reduced, and anti-bacteria fresh keeping performance of the packaging material is obviously improved.

The invention relates to a preparation method and application of a magnetic silicon dioxide composite microsphere. Superparamagnetic ferroferric oxide nano particles of which the diameter ranges from 4 nm to 30 nm are prepared through a high-temperature pyrolysis method, a silicon dioxide shell of which the thickness ranges from 5 nm to 20nm covers the outer surfaces of the magnetic ferroferric oxide nano particles through a reverse microemulsion method, amination modification is conducted on the silicon dioxide surface, glutaraldehyde is used as a crosslinking arm, ligand protein is connected into, and protein separation is conducted through specific binding of ligand protein and target protein. The prepared magnetic microsphere is small in particle diameter and good in monodispersity, the composite microsphere with amine is large in specific surface area, nucleophilic addition is utilized, after the crosslinking arm glutaraldehyde is connected into, multiple kinds of ligand protein can be connected into, and then multiple kinds of target protein can be separated. The method is suitable for rapid separation and application of protein in biological samples, and has wide application prospect and great application value in the biomedical field and other fields.

The invention supplies a biodegradable nanometer channel multi orifice stanching material and manufacturing method which uses chitosan as raw material. It includes orifice making, forming, orifice making agent removing. The multi orifice structure can be gained by adding orifice making agent in preparation, removing it in forming, which emulsifies to form latex, solidifies to form hydrogel, removes the agent by alcohol or ketone solvent, dries the hydrogel, or processes cross linking for the formaldehyde, glutaraldehyde, solidifies chitosan aqueous solution to form hydrogel, removes the agent by water, dries the hydrogel. This material can fast adsorb the moisture in the blood to stanch.

The invention relates to a PVA hydrogel-based photo-thermal evaporation material and preparation and application thereof. The preparation method comprises the following steps: step 1, respectively preparing an aqueous polyvinyl alcohol (PVA) solution, an aqueous nanometer photo-thermal material dispersion solution, an aqueous glutaraldehyde solution and a low-concentration hydrochloric acid solution; 2, fully mixing and stirring the aqueous PVA solution, the aqueous nanometer photo-thermal material dispersion solution, the aqueous glutaraldehyde solution and the low-concentration hydrochloricacid solution in the step 1 according to a certain sequence under a certain temperature condition; and 3, transferring the mixed and stirred product into a forming mold when the mixed and stirred product is not cured, and carrying out standing until a cross-linking reaction is complete, thereby obtaining the hydrogel material. The preparation method disclosed by the invention has the beneficial effects that preparation process is environment-friendly, pollution-free, simple and feasible; preparation cost is low; the obtained hydrogel can be used as an ideal photo-thermal evaporation and solarseawater desalination material; and the prepared hydrogel has high light absorption efficiency, photo-thermal conversion efficiency and evaporation efficiency, and can be applied to the field of solarseawater desalination.

The invention relates to a metal-organic framework supported heteropoly acid catalyst for synthesizing glutaraldehyde and a production method of the metal-organic framework supported heteropoly acid catalyst. The catalyst is prepared by a one-step synthesis method, namely a heteropoly acid component with catalytic oxidation activity is introduced into a duct of a metal-organic framework material UiO-66 in the process of synthesizing the metal-organic framework material UiO-66. The catalyst has the characteristics that the catalyst has a crystal framework structure, the active component is highly dispersed, and the heteropoly acid is over-high in load. The catalyst is applied to catalytic selective oxidation reaction of cyclopentene, so that the selectivity and the yield of the glutaraldehyde is greatly improved; the numerical value is much higher than the reported homogeneous catalysis level; and the metal-organic framework supported heteropoly acid catalyst has important industrial application value.

The invention discloses a method for removing sulfonic-group-containing dye in alkaline waste water by using a magnetic chitosan adsorbent, which comprises the following steps: (1) dissolving chitosan in an iron chloride water solution, stirring, adding ethanol to precipitate a solid, filtering out the solid, washing, mixing the solid with nano magnetic ferroferric oxide, carrying out cross-linking reaction with a glutaric dialdehyde water solution, and carrying out after-treatment to obtain a magnetic chitosan adsorbent; (2) adding the obtained magnetic chitosan adsorbent into an alkaline waste water solution containing the sulfonic-group-containing dye, stirring, carrying out magnetic separation, and drying to obtain the dye-adsorbed magnetic chitosan adsorbent; and (3) adding the dye-adsorbed magnetic chitosan adsorbent into a water solution containing a desorbing agent, stirring, and filtering to obtain a dye recovered solution and the recovered magnetic chitosan adsorbent. The method disclosed by the invention is simple to operate, has the advantages of environment friendliness and low cost, and has wide industrial application prospects.

The invention relates to a preparation method of a magnetic chitosan composite microsphere adsorbent, which belongs to the technical field of environmental material preparation. The preparation method comprises the following steps: firstly with FeCl3. 6H2O and FeCl2.4H2O as raw materials, preparing nano Fe3O4 by a coprecipitation process; and secondly, with glutaraldehyde as a crosslinking agent and Fe3O4, kaolin and chitosan as raw materials, synthesizing the novel magnetic chitosan composite microsphere adsorbent by an emulsification crosslinking process. The magnetic chitosan composite microsphere adsorbent is used for separating ciprofloxacin hydrochloride out of a water environment. The adsorption performance and the dynamic performance of the prepared adsorbent are researched in a static adsorption experiment. The results show that the novel magnetic chitosan composite microsphere adsorbent obtained by the method has a rapid adsorption dynamics characteristic, a high adsorption capacity and high magnetic-separation efficiency.

The invention discloses an ECL (electrochemiluminescence) immunosensor for detecting tumor markers and a preparation method and applications thereof. The immunosensor comprises a working electrode, a reference electrode and a counter electrode, wherein the working electrode is a gold electrode, cysteamine, glutaraldehyde, a tumor marker first-antibody, a tumor marker, and a composite functionalized nanosphere are sequentially decorated on the surface of the gold electrode, the composite functionalized nanosphere is simultaneously marked by a tumor marker second-antibody and an ECL marker, and non-specific active sites are closed by using bovine serum albumins. The preparation method comprises the steps of preparing the gold electrode on which the tumor marker first-antibody is fixedly carried; synthesizing the composite functionalized nanosphere simultaneously marked by the tumor marker second-antibody and the ECL marker; and finally, assembling the obtained gold electrode and the composite functionalized nanosphere into an ECL immunosensor. The ECL immunosensor disclosed by the invention is high in sensitivity, rapid in analysis speed, strong in stability and selectivity, good in reproducibility, easy to operate, and flexible in method.

A method of preparing an antigen-immobilized immuno-fluorescence slide, the method comprising: immobilizing a C-reactive protein on a slide to prepare a protein chip; mixing an antibody that specifically binds to a target protein, with streptavidin to label the antibody with a fluorescent nanoparticle; immuno-reacting the antibody by competitive mixing, assaying with a fluorescence camera, wherein the immobilizing of the C-reactive protein on the slide comprises: modifying the slide with 3-aminopropyltrimethoxysilane to prepare a modified slide; hydrating the slide modified with 3-aminopropyltrimethoxysilane; activating the modified slide by using a glutaraldehyde solution; dissolving a C-reactive protein at a concentration of 0.01-0.5 mg/ml in a 30-70 mM phosphate buffer solution (pH 6.5-7.8) to prepare an antigen solution for immobilization; placing a petri dish comprising the slide on a spotting guide and spotting 1-100 μl of the antigen solution on spotting points; and performing a reaction on the slide prepared as described above for 1-6 hours to immobilize the antigen, and an immune-fluorescence slide prepared by using the method.

The invention relates to a formula and method for immobilizing a tick sample for scanning electron microscopy, which belongs to the technical field of methods for immobilizing samples for scanning electron microscopy. The immobilization formula comprises a PBS buffer solution, polysorbate and glutaraldehyde, and a glutaraldehyde immobilization liquid is prepared from the above-mentioned components. The method comprises the following steps: (1) observing whether host tissue is left in basis capituli of the tick sample and if so, removing the host tissue; (2) cleaning the surface of the tick sample; (3) immobilizing the tick sample at room temperature by using the glutaraldehyde immobilization liquid; (4) carrying out gradient dehydration with ethanol on the immobilized tick sample; (5) putting the tick sample into mixed liquor of absolute ethyl alcohol and acetone for displacement, and then putting the tick sample into acetone for displacement; and (6) drying the tick sample in a vacuum freeze drying instrument for drying and spraying gold on the tick sample. During scanning electron microscopic observation, the phenomenon of fuzzy and foggy images or incapable imaging due to ionization discharging of the sample as water vapor produced after electron beam bombardment of the sample encounters high-energy electron streams is prevented.

The invention provides a method for preparing a macroporous reticulated polyvinyl alcohol spherical vector. The method comprises the following steps: firstly, polyvinyl alcohol, calcium carbonate, sodium alginate and water are mixed in a certain mass proportion, and stirring is performed for full dissolution of the polyvinyl alcohol and uniform mixture, and then white PVA sol is obtained; secondly, the sol obtained is added into saturated boric acid solution containing 3 percent of calcium chloride by a peristaltic pump, and PVA gel beads are formed, cleaned by water and then placed into diluted hydrochloric acid solution for dipping until no air bubble is generated; thirdly, a spherical vector is thrown into glutaral pentanedial water solution and adjusted into acidity so as to generate crosslinking reaction to form a more stable crosslinking structure; and fourthly, the spherical vector is dipped into water and cleaned into neutrality, and then the white macroporous reticulated PVA spherical vector with elasticity is obtained. The macroporous reticulated polyvinyl alcohol vector prepared by the method has stable macroporous reticulated structure and good hydrophilicity, physical and chemical stability and anti-biological degradability, is suitable for immobilized enzymes and microorganisms so as to form a plurality of bed-shaped bioreactors, and is used in the modern bioengineering field such as sewage treatment and so on.

The invention relates to a preparation method for a novel magnetic chitosan composite microsphere antibiotic adsorbent, belonging to the technical field of preparing environment materials. The preparation method comprises the following steps: by taking FeCl3.6H2O, FeCl2.4H2O and halloysite nanotubes HNTs as raw materials, preparing Fe3O4/HNTs compound through co-precipitation, then by taking glutaraldehyde as a crosslinking agent and Fe3O4/HNTs compound and chitosan as raw materials, synthesizing the magnetic chitosan composite microsphere adsorbent by adopting an emulsification crosslinking method, and separating tetracycline (TC) in water environment. The static adsorption experiments are used for researching the adsorption balance and dynamics performance of the prepared adsorbent. Results show that the obtained novel magnetic chitosan composite microsphere antibiotic adsorbent has fast adsorption dynamics property, high adsorption capacity and high magnetic separation capability.

The invention provides PAAm/PVA dual-network hydrogel and a preparation method thereof. The dual-network hydrogel is PAAm/PVA dual-network hydrogel based on acrylamide (AAm) and polyvinyl alcohol (PVA), which is high in strength and high in moisture content. The dual-network hydrogel is prepared by the following steps: dissolving the polyvinyl alcohol into water to obtain a solution S1; dissolving the acrylamide, a crosslinking agent and an initiator into water to obtain a solution S2; mixing the solution S1 with the solution S2 at a certain ratio to obtain a solution S3; and obtaining the PAAm/PVA dual-network hydrogel through hot or/and light or/and redox initiated polymerization. The dual-network hydrogel can also be further crosslinked through the crosslinking agents such as glutaraldehyde and sodium borate to change the mechanical property. The PAAm/PVA dual-network hydrogel has the characteristics of being high in balanced water absorption rate (EWC), high in strength, convenient to prepare, and low in swelling degree, and is molded once.

The invention provides a microcapsule sustained release agent and a preparation method and application thereof. The microcapsule sustained release agent is prepared by embedding essence with gelatin and Arabic gum, and is applied to sanitary towels or sanitary cushions or panty-shape diapers or pet cushions. The microcapsule sustained release agent consists of a core material and a wall material, wherein the wall material consists of natural high molecular gelatin, Arabic gum and a curing agent; the core material consists of essence and essential oil; the curing agent consists of tannic acid and glutaraldehyde; and microencapsulation is performed on the core material and the wall material in the ratio 1:2. The invention further provides a preparation method of the microcapsule sustained release agent. The preparation method comprises the following steps of: adding an emulsifier into Arabic gum for mixing; adding essence or essential oil into an emulsion for homogenizing and emulsifying; adding a gelatin solution into the emulsified emulsion; agglomerating into a capsule; curing with a curing agent; and drying to obtain a high-dispersity microcapsule sustained release agent. By adding the microcapsule sustained release agent into the space between a skin contact layer and a water-absorbing layer of a sanitary towel or sanitary cushion or panty-shape diaper or pet cushion, the effect of keeping fragrance for a long time can be achieved.

The invention relates to a photocatalytic composite gel bead and a preparation method as well as an application of the gel bed. The composite bead takes crosslinked alginate as a matrix, and the matrix is filled with titanium dioxide nanometer particles. The preparation method specifically comprises the following steps of: firstly mixing the titanium dioxide, the alginate and water uniformly, dropwise adding the mixed solution into a calcium chloride solution by using an injector to prepare uniform beads, collecting and washing the beads, adding the washed beads into a solution of glutaraldehyde and hydrochloric acid to carry out a crosslinking reaction, and finally collecting and washing the beads to obtain the final product. The product has the advantages of low cost, simple preparation, good light degradation effect, easy separation and recycling and the like, has a good light degradation effect on hexavalent chromium, and can be applied to the treatment of chromium containing wastewater discharged from an electroplating factory, an electronic equipment factory, a metallurgical refinery and the like.

The invention relates to a thin-film material for an optical fiber biosensor probe and a preparation method thereof. The material has catalytic characteristic and photosensitivity and is made from Fe3O4/SiO2 magnetic nanocomposite particles, bioactive enzyme, fluorescent material and sol-gel. The bioactive enzyme is fixed on the Fe3O4/SiO2 magnetic nanocomposite particles and the fixed bioactive enzyme and the fluorescent material are embedded in a sol-gel grid. The preparation method comprises the following steps: a Fe3O4/SiO2 magnetic nanocomposite particle carrier is synthesized; the carrier is activated by glutaraldehyde or by glutaraldehyde, bovine serum albumin and glutaraldehyde in sequence and then has a crosslinking reaction with the bioactive enzyme to fix the enzyme; the Fe3O4/SiO2 magnetic nanocomposite particles fixed with the bioactive enzyme are dripped on the surface of a treated glass substrate after being mixed with the sol-gel liquid blended with a Ru(bpy)3Cl2 fluorescent indicator; after cleaning, drying and aging, a sol-gel optical bio-composite sensing film embedding the bioactive enzyme and the fluorescent indicator is obtained. The optical fiber biosensor probe made from the thin-film material is especially applicable to the detection of blood sugar, blood cholesterol and the like.