1925 results about "Glutaraldehyde" patented technology

The invention discloses a method of treating a patient for pleural effusion comprising percutaneously delivering an adhesive material to a pleural space of the patient. Suitable adhesive materials for performing any of the embodiments of the methods of the invention can be selected from the group consisting of hydrogels, collagen, poly(lactic acid), poly(glycolide), cyanoacrylates, glutaraldehyde, PEG, protein, and polysaccharide and derivatives thereof. The invention also discloses a pleural effusion treatment apparatus comprising an adhesive material adapted to adhere pleural membranes defining a pleural space and a pleural space access member adapted to deliver the adhesive material to the pleural space.

Methods for decellularizing mammalian tissue for use in transplantation and tissue engineering. The invention includes methods for simultaneous application of an ionic detergent and a nonionic detergent for a long time period, which may exceed five days. One method utilizes SDS as the ionic detergent and Triton-X 100 as the nonionic detergent. A long rinse step follows, which may also exceed five days in length. This long duration, simultaneous extraction with two detergents produced tissue showing stress-strain curves and DSC data similar to that of fresh, unprocessed tissue. The processed tissue is largely devoid of cells, has the underlying structure essentially intact, and also shows a significantly improved inflammatory response relative to fresh tissue, even without glutaraldehyde fixation. Significantly reduced in situ calcification has also been demonstrated relative to glutaraldehyde fixed tissue. Applicants believe the ionic and non-ionic detergents may act synergistically to bind protein to the ionic detergent and may remove an ionic detergent-protein complex from the tissue using the non-ionic detergent. The present methods find one exemplary use in decellularizing porcine heart valve leaflet and wall tissue for use in transplantation.

Biocompatible phase invertable proteinaceous compositions and methods for making and using the same are provided. The subject phase invertable compositions are prepared by combining a proteinaceous substrate and a cross-linker. The proteinaceous substrate includes one or more proteins and an adhesion modifier, and may also include one or more of: a pasticizer, a carbohydrate, or other modification agent. In certain embodiments, the cross-linker is a heat-treated dialdehyde, e.g., heat-treated glutaraldehyde. Also provided are kits for use in preparing the subject compositions. The subject compositions, kits and systems find use in a variety of different applications.

Biocompatible phase invertable proteinaceous compositions and methods for making and using the same are provided. The subject phase invertable compositions are prepared by combining a proteinaceous substrate and a cross-linker. The proteinaceous substrate includes one or more proteins and an adhesion modifier, and may also include one or more of: a pasticizer, a carbohydrate, or other modification agent. In certain embodiments, the cross-linker is a heat-treated dialdehyde, e.g., heat-treated glutaraldehyde. Also provided are kits for use in preparing the subject compositions. The subject compositions, kits and systems find use in a variety of different applications.

A method of treating a biological tissue including contacting the biological tissue with an aqueous sterilizing solution, and maintaining the aqueous sterilizing solution at a temperature of about 50° C. for a time period of about 1 to 2 days. The method of treating a biological tissue may be utilized as a terminal sterilization step in a method for fixation of biological tissues, and bioprosthetic devices may be prepared by such fixation method. The fixation method may include the steps of A) fixing the tissue, B) treating the tissue with a mixture of i) a denaturant, ii) a surfactant and iii) a crosslinking agent, C) fabricating or forming the bioprosthesis (e.g., forming the tissue and attaching any non-biological components thereto) and D) subjecting the bioprosthesis to the terminal sterilization method. The aqueous sterilizing solution may be glutaraldehyde of about 0.625 weight percent buffered to a pH of about 7.4.

The invention relates to a method for preparing ethylenediamine modified chitosan composite magnetic microspheres and application thereof. The method specifically comprises the following steps of: mixing acidic aqueous solution of chitosan and a water-based magnetofluid first, then crosslinking by using glutaraldehyde, then adjusting the pH value to form a gelatinous precipitate, then modifying by using ethylenediamine and epichlorohydrin, and washing and drying to obtain a final product. The product has good absorption effect on radionuclide aranium and metal ions such as heavy metal Pb, Cr and the like; the removal rate reaches over 95 percent when the initial concentration of various ions is within the range of 200mg / L; and the product has quick absorption rate and good regeneration performance. The ethylenediamine modified chitosan composite magnetic microspheres can be used for metal recovery and pollution remediation in mines, radioactive waste water, smelteries, electronics factories and waste water of electroplating factories.

The invention relates to a polyvinyl alcohol / chitosan nano fiber film dressing containing nano silver and preparation thereof. The nano fiber film dressing is prepared by the following steps: taking a mixture of polyvinyl alcohol and chitosan as the base material, then mixing nano silver into the polyvinyl alcohol / chitosan nano fiber film through an electrostatic spinning technology, and finally adding glutaraldehyde to crosslink the nano fiber film so as to obtain the target product. The obtained dressing has the characteristics that the preparation technology is simple; a wet environment can be achieved for promoting wound healing; and the dressing has an antiseptic function.

The invention relates to a preparation method of a chitosan compound flocculant which can effectively remove organic pollutants in water, can adsorb heavy metal ions such as nickel, chromium and lead in water and has high heavy metal ion adsorption capacity and high adsorption efficiency and a chitosan compound flocculant product thereof. The chitosan compound flocculant product is prepared by adopting chitosan as a raw material, glutaraldehyde as a crosslinking agent and washed and acid pickled attapulgite as a modifier. The preparation method of the compound flocculant capable of adsorbing heavy metal ions has the characteristics of simple technology, low cost, no special equipment and easy industrial implementation, the compound flocculant reduces dosage of chiston, and cost is reduced.

An endotoxin adsorbent used for blood perfusion is prepared from agat through adding the aqueous solution to the liquid mixture of toluene and CCl4 to obtain spherical agar, activating by epoxy chloropropane under alkaline condition, reacting on diamine and then on glutaraldehyde to obtain high-strength agar beads used as the carrier of adsorbent, reacting on the amino contained ligand (polymyxin B), and reducing by NaBH4. Its advantages are high compatibility to blood and high effect on absorbing endotoxin from blood.

The invention relates to a preparation method of a protein magnetic imprinting nano sphere, in particular to a preparation method of a protein magnetic imprinting nano sphere which takes one of hemoglobin, muramidase or serum protein as template molecules; the preparation method includes the following steps: synthesizing magnetic nano particles which are Fe3O4 nano particles covered with silicon dioxide on the surfaces; carrying out silanization to the amino-groups on the surface of the magnetic sphere; using glutaric dialdehyde to connect protein; using silylation agent to fix the space structure of template protein; alkaline eluting the protein on the surface of the magnetic sphere to form imprinting sites. The hemoglobin, muramidase or serum protein magnetic nano imprinting polymer sphere prepared by the invention combines the precise and specific identification property of molecular imprinting and the quick separation property of the magnetic nano sphere under the action of an external magnetic field, integrates the advantages of molecular imprinting and the magnetic nano sphere, avoids complex centrifugation operation, plays vital roles in the separation of cells, protein and nucleic acid, the detection of biomolecules, tumor diagnosis and medicine targeting therapy and has promising application prospect in the field of isolation analysis.

The invention relates to a polyvinyl alcohol radical composite coating fresh keeping packaging material nano TiO2 functional modification method, which comprises the following steps: adding nano TiO2 and SiO2 into a polyvinyl alcohol aqueous solution and stirring; carrying out ultrasonic mixing on a mixed liquor; placing the mixed solution in a reaction kettle after ultrasonic; heating, stirring and reacting at constant temperature; adding stearic acid-alcohol mixed solution and glutaraldehyde with the same temperature and stirring; and carrying out cross-linking reaction at a constant temperature; stirring and cooling to a room temperature after reaction, so as to prepare into a nano composite coating new material. With the adoption of the polyvinyl alcohol radical composite coating fresh keeping packaging material nano TiO2 functional modification method, the nano TiO2 is adopted to modify a polyvinyl alcohol radical composite coating fresh keeping packaging material; the new material film formation moisture permeability is obviously reduced, and anti-bacteria fresh keeping performance of the packaging material is obviously improved.

The invention relates to a preparation method and application of a magnetic silicon dioxide composite microsphere. Superparamagnetic ferroferric oxide nano particles of which the diameter ranges from 4 nm to 30 nm are prepared through a high-temperature pyrolysis method, a silicon dioxide shell of which the thickness ranges from 5 nm to 20nm covers the outer surfaces of the magnetic ferroferric oxide nano particles through a reverse microemulsion method, amination modification is conducted on the silicon dioxide surface, glutaraldehyde is used as a crosslinking arm, ligand protein is connected into, and protein separation is conducted through specific binding of ligand protein and target protein. The prepared magnetic microsphere is small in particle diameter and good in monodispersity, the composite microsphere with amine is large in specific surface area, nucleophilic addition is utilized, after the crosslinking arm glutaraldehyde is connected into, multiple kinds of ligand protein can be connected into, and then multiple kinds of target protein can be separated. The method is suitable for rapid separation and application of protein in biological samples, and has wide application prospect and great application value in the biomedical field and other fields.

The invention supplies a biodegradable nanometer channel multi orifice stanching material and manufacturing method which uses chitosan as raw material. It includes orifice making, forming, orifice making agent removing. The multi orifice structure can be gained by adding orifice making agent in preparation, removing it in forming, which emulsifies to form latex, solidifies to form hydrogel, removes the agent by alcohol or ketone solvent, dries the hydrogel, or processes cross linking for the formaldehyde, glutaraldehyde, solidifies chitosan aqueous solution to form hydrogel, removes the agent by water, dries the hydrogel. This material can fast adsorb the moisture in the blood to stanch.

The invention relates to a PVA hydrogel-based photo-thermal evaporation material and preparation and application thereof. The preparation method comprises the following steps: step 1, respectively preparing an aqueous polyvinyl alcohol (PVA) solution, an aqueous nanometer photo-thermal material dispersion solution, an aqueous glutaraldehyde solution and a low-concentration hydrochloric acid solution; 2, fully mixing and stirring the aqueous PVA solution, the aqueous nanometer photo-thermal material dispersion solution, the aqueous glutaraldehyde solution and the low-concentration hydrochloricacid solution in the step 1 according to a certain sequence under a certain temperature condition; and 3, transferring the mixed and stirred product into a forming mold when the mixed and stirred product is not cured, and carrying out standing until a cross-linking reaction is complete, thereby obtaining the hydrogel material. The preparation method disclosed by the invention has the beneficial effects that preparation process is environment-friendly, pollution-free, simple and feasible; preparation cost is low; the obtained hydrogel can be used as an ideal photo-thermal evaporation and solarseawater desalination material; and the prepared hydrogel has high light absorption efficiency, photo-thermal conversion efficiency and evaporation efficiency, and can be applied to the field of solarseawater desalination.

Fixed-dried red blood cells (RBCs), and processes for preparing the same are disclosed. The red blood cells, upon reconstitution with distilled water or appropriate buffer: bind oxygen with native affinities, have partial deformability, present minimal thrombogenicity to platelets, and have oblated blood group antigens. The RBCs are preferably fixed by means of cross-linkers with aldehyde functions such as paraformaldehyde or glutaraldehyde either alone or in combination. Native oxygen kinetics are achieved by preparing the red blood cells with 1,6-diphosphofructose. Blood group antigens and chemical functions that render the lyophilized RBCs thrombogenic are occluded by chemically attaching polyoxyethylene glycol polymers to the surface membrane of the red blood cells. The cross-linked red blood cells are preferably died by lyophilization.

The invention relates to a metal-organic framework supported heteropoly acid catalyst for synthesizing glutaraldehyde and a production method of the metal-organic framework supported heteropoly acid catalyst. The catalyst is prepared by a one-step synthesis method, namely a heteropoly acid component with catalytic oxidation activity is introduced into a duct of a metal-organic framework material UiO-66 in the process of synthesizing the metal-organic framework material UiO-66. The catalyst has the characteristics that the catalyst has a crystal framework structure, the active component is highly dispersed, and the heteropoly acid is over-high in load. The catalyst is applied to catalytic selective oxidation reaction of cyclopentene, so that the selectivity and the yield of the glutaraldehyde is greatly improved; the numerical value is much higher than the reported homogeneous catalysis level; and the metal-organic framework supported heteropoly acid catalyst has important industrial application value.

The invention discloses a method for removing sulfonic-group-containing dye in alkaline waste water by using a magnetic chitosan adsorbent, which comprises the following steps: (1) dissolving chitosan in an iron chloride water solution, stirring, adding ethanol to precipitate a solid, filtering out the solid, washing, mixing the solid with nano magnetic ferroferric oxide, carrying out cross-linking reaction with a glutaric dialdehyde water solution, and carrying out after-treatment to obtain a magnetic chitosan adsorbent; (2) adding the obtained magnetic chitosan adsorbent into an alkaline waste water solution containing the sulfonic-group-containing dye, stirring, carrying out magnetic separation, and drying to obtain the dye-adsorbed magnetic chitosan adsorbent; and (3) adding the dye-adsorbed magnetic chitosan adsorbent into a water solution containing a desorbing agent, stirring, and filtering to obtain a dye recovered solution and the recovered magnetic chitosan adsorbent. The method disclosed by the invention is simple to operate, has the advantages of environment friendliness and low cost, and has wide industrial application prospects.

The invention relates to a composite type mercury ion adsorbent and a preparation method thereof. The adsorbent consists of chitosan, polyvinyl alcohol and clay. A polyvinyl alcohol macromolecular chain and a chitosan macromolecular chain are penetrated and wound mutually by high-speed stirring, so that the inorganic clay is dispersed fully; and the release of the chitosan and the polyvinyl alcohol in the process of using the adsorbent is avoided effectively by a method of combining cross linking agent cross linking and freezing crossing linking of glutaraldehyde. The adsorbent has high adsorption capacity and high adsorptive selectivity on Hg(II) ions, hardly adsorbs Cd(II) and Pb(II) ions, and adsorbs a small amount of Cu(II) ions, and can be recycled, and adsorbability of the adsorbentis slightly influenced by the pH value of a solution. The integral preparation process is performed in an aqueous solution, the preparation method is simple and convenient, low in energy consumption,low in production cost, environment-friendly, and easy to industrialize, and special process equipment is avoided.

The invention relates to an optimized preparation method of oxidized graphite paper, belonging to the technical field of nanomaterials. The preparation method comprises the following steps: preparing graphene oxide by adopting hydrogen peroxide; then, adding a glutaric dialdehyde solution into the suspension of the graphene oxide; and casting and drying on a substrate after stirring to obtain the oxidized graphite paper with enhanced mechanical properties. The method of the invention is simple and easy, is suitable for large-scale preparation, and can realize large-scale preparation of the oxidized graphite paper. By adding a small amount of cross-linking agent, the obtained oxidized graphite paper with enhanced mechanical properties has regular structure, uniform height and enough mechanical strength.

The invention relates to a preparation method of a magnetic chitosan composite microsphere adsorbent, which belongs to the technical field of environmental material preparation. The preparation method comprises the following steps: firstly with FeCl3. 6H2O and FeCl2.4H2O as raw materials, preparing nano Fe3O4 by a coprecipitation process; and secondly, with glutaraldehyde as a crosslinking agent and Fe3O4, kaolin and chitosan as raw materials, synthesizing the novel magnetic chitosan composite microsphere adsorbent by an emulsification crosslinking process. The magnetic chitosan composite microsphere adsorbent is used for separating ciprofloxacin hydrochloride out of a water environment. The adsorption performance and the dynamic performance of the prepared adsorbent are researched in a static adsorption experiment. The results show that the novel magnetic chitosan composite microsphere adsorbent obtained by the method has a rapid adsorption dynamics characteristic, a high adsorption capacity and high magnetic-separation efficiency.

The invention discloses a method for finishing an antistatic antibacterial and hydrophilic polyester fabric. The finishing method comprises the following steps: step (10) preparing an N,O-carboxymethyl chitosan solution; step (20) performing pretreatment on the polyester fabric by adopting cold plasma; and step (30) dipping the polyester fabric treated in the step (20) by utilizing a polyethyleneimine solution, then dipping the polyester fabric by utilizing a glutaraldehyde solution, and finally, dipping the polyester fabric by utilizing the N,O-carboxymethyl chitosan solution prepared in the step (10), thereby obtaining the treated polyester fabric. According to the finishing method, the polyester fabric has good antistatic antibacterial and hydrophilic performances.

The invention discloses an ECL (electrochemiluminescence) immunosensor for detecting tumor markers and a preparation method and applications thereof. The immunosensor comprises a working electrode, a reference electrode and a counter electrode, wherein the working electrode is a gold electrode, cysteamine, glutaraldehyde, a tumor marker first-antibody, a tumor marker, and a composite functionalized nanosphere are sequentially decorated on the surface of the gold electrode, the composite functionalized nanosphere is simultaneously marked by a tumor marker second-antibody and an ECL marker, and non-specific active sites are closed by using bovine serum albumins. The preparation method comprises the steps of preparing the gold electrode on which the tumor marker first-antibody is fixedly carried; synthesizing the composite functionalized nanosphere simultaneously marked by the tumor marker second-antibody and the ECL marker; and finally, assembling the obtained gold electrode and the composite functionalized nanosphere into an ECL immunosensor. The ECL immunosensor disclosed by the invention is high in sensitivity, rapid in analysis speed, strong in stability and selectivity, good in reproducibility, easy to operate, and flexible in method.

A method of preparing an antigen-immobilized immuno-fluorescence slide, the method comprising: immobilizing a C-reactive protein on a slide to prepare a protein chip; mixing an antibody that specifically binds to a target protein, with streptavidin to label the antibody with a fluorescent nanoparticle; immuno-reacting the antibody by competitive mixing, assaying with a fluorescence camera, wherein the immobilizing of the C-reactive protein on the slide comprises: modifying the slide with 3-aminopropyltrimethoxysilane to prepare a modified slide; hydrating the slide modified with 3-aminopropyltrimethoxysilane; activating the modified slide by using a glutaraldehyde solution; dissolving a C-reactive protein at a concentration of 0.01-0.5 mg / ml in a 30-70 mM phosphate buffer solution (pH 6.5-7.8) to prepare an antigen solution for immobilization; placing a petri dish comprising the slide on a spotting guide and spotting 1-100 μl of the antigen solution on spotting points; and performing a reaction on the slide prepared as described above for 1-6 hours to immobilize the antigen, and an immune-fluorescence slide prepared by using the method.

The invention relates to a formula and method for immobilizing a tick sample for scanning electron microscopy, which belongs to the technical field of methods for immobilizing samples for scanning electron microscopy. The immobilization formula comprises a PBS buffer solution, polysorbate and glutaraldehyde, and a glutaraldehyde immobilization liquid is prepared from the above-mentioned components. The method comprises the following steps: (1) observing whether host tissue is left in basis capituli of the tick sample and if so, removing the host tissue; (2) cleaning the surface of the tick sample; (3) immobilizing the tick sample at room temperature by using the glutaraldehyde immobilization liquid; (4) carrying out gradient dehydration with ethanol on the immobilized tick sample; (5) putting the tick sample into mixed liquor of absolute ethyl alcohol and acetone for displacement, and then putting the tick sample into acetone for displacement; and (6) drying the tick sample in a vacuum freeze drying instrument for drying and spraying gold on the tick sample. During scanning electron microscopic observation, the phenomenon of fuzzy and foggy images or incapable imaging due to ionization discharging of the sample as water vapor produced after electron beam bombardment of the sample encounters high-energy electron streams is prevented.

The invention provides a method for preparing a macroporous reticulated polyvinyl alcohol spherical vector. The method comprises the following steps: firstly, polyvinyl alcohol, calcium carbonate, sodium alginate and water are mixed in a certain mass proportion, and stirring is performed for full dissolution of the polyvinyl alcohol and uniform mixture, and then white PVA sol is obtained; secondly, the sol obtained is added into saturated boric acid solution containing 3 percent of calcium chloride by a peristaltic pump, and PVA gel beads are formed, cleaned by water and then placed into diluted hydrochloric acid solution for dipping until no air bubble is generated; thirdly, a spherical vector is thrown into glutaral pentanedial water solution and adjusted into acidity so as to generate crosslinking reaction to form a more stable crosslinking structure; and fourthly, the spherical vector is dipped into water and cleaned into neutrality, and then the white macroporous reticulated PVA spherical vector with elasticity is obtained. The macroporous reticulated polyvinyl alcohol vector prepared by the method has stable macroporous reticulated structure and good hydrophilicity, physical and chemical stability and anti-biological degradability, is suitable for immobilized enzymes and microorganisms so as to form a plurality of bed-shaped bioreactors, and is used in the modern bioengineering field such as sewage treatment and so on.

The invention provides PAAm / PVA dual-network hydrogel and a preparation method thereof. The dual-network hydrogel is PAAm / PVA dual-network hydrogel based on acrylamide (AAm) and polyvinyl alcohol (PVA), which is high in strength and high in moisture content. The dual-network hydrogel is prepared by the following steps: dissolving the polyvinyl alcohol into water to obtain a solution S1; dissolving the acrylamide, a crosslinking agent and an initiator into water to obtain a solution S2; mixing the solution S1 with the solution S2 at a certain ratio to obtain a solution S3; and obtaining the PAAm / PVA dual-network hydrogel through hot or / and light or / and redox initiated polymerization. The dual-network hydrogel can also be further crosslinked through the crosslinking agents such as glutaraldehyde and sodium borate to change the mechanical property. The PAAm / PVA dual-network hydrogel has the characteristics of being high in balanced water absorption rate (EWC), high in strength, convenient to prepare, and low in swelling degree, and is molded once.