551 results about "Glutaraldehyde" patented technology

The invention discloses a solar interface evaporator and a preparation method and application thereof. The evaporator comprises a porous cellulose supporting layer and a photo-thermal composite hydrogel layer loaded on the cellulose supporting layer, and the porous cellulose supporting layer and the photo-thermal composite hydrogel layer are combined in a gel in-situ cross-linking mode to form an integrated composite structure. The photo-thermal composite hydrogel layer is composite hydrogel formed by cross-linking polyvinyl alcohol, chitosan quaternary ammonium salt and carbon black. The preparation method comprises the following steps: adding polyvinyl alcohol and chitosan quaternary ammonium salt into a solvent to form a transparent mixed solution, adding the carbon-based photothermal conversion material, and continuously heating and stirring to prepare a hydrogel precursor solution; then adding glutaraldehyde and hydrochloric acid, and carrying out cross-linking reaction to form a hydrogel system; and immersing the cellulose sponge, standing, circularly and alternately freezing and unfreezing, and finally freeze-drying to obtain the product. The evaporator disclosed by the invention integrates highlight absorption, high heat utilization, water transportation, salt resistance and antibiosis, can realize large-scale production, and is suitable for scenes such as seawater desalination, sewage purification and emergency water supply.

The invention discloses a fruit and vegetable exosome extraction method based on ligand fishing. The fruit and vegetable exosome extraction method comprises the following steps: suspending amino-functionalized Fe3O4 (at) SiO2 nanoparticles in a glutaraldehyde solution, and adding activated agglutinin to obtain Fe3O4 (at) SiO2-agglutinin nanoparticles; adding a glutaraldehyde solution into agarose gel to obtain a size exclusion chromatographic column filler; the method comprises the following steps: dispersing Fe3O4 (at) SiO2-lectin nanoparticles in a PBS buffer solution to obtain a uniform suspension, adding the uniform suspension into a size exclusion chromatographic column filler to obtain a uniform slurry, filling the size exclusion chromatographic column with the uniform slurry, and flushing with the buffer solution to form a magnetic bead stationary phase; and loading fruit and vegetable supernatant into a size exclusion chromatographic column, passing through the magnetic bead stationary phase, cleaning residual impurities combined on the exosome-magnetic bead compound by using a buffer solution, and dissociating the exosome from the magnetic beads by using a competitive eluent of the exosome to obtain the high-purity fruit and vegetable exosome. The problem that an existing exosome extraction method is difficult to meet the requirements of high efficiency, simplicity, high purity and high activity at the same time is solved.

The invention discloses a biomass-inorganic-organic ternary composite flocculant and a preparation method and application thereof, and belongs to the field of coking wastewater treatment.The preparation method comprises the steps that chitosan serves as a substrate material, glutaraldehyde serves as a cross-linking agent, and polyferric sulfate (PFS) and chitosan are subjected to chemical cross-linking to generate an intermediate product PFS and chitosan composite particles; then potassium persulfate is used as an initiator, graft copolymerization of acrylamide and methacryloyloxyethyl trimethyl ammonium chloride is initiated through hydroxyl on the surface of PFS-chitosan, a cationic branched chain is introduced, and a final product is obtained. Through multi-component cooperation and structural design, the defects that a traditional flocculating agent is not thorough in demulsification, large in sludge amount, unstable in floc combination and the like in coking wastewater treatment are overcome, and the flocculating agent is particularly suitable for treating oil pollutants in coking wastewater.

The invention belongs to the technical field of membrane separation, and particularly relates to a method for preparing a high-flux hollow fiber nanofiltration membrane based on interfacial polymerization, the obtained membrane and application. The method comprises the following steps: firstly, carrying out pre-adsorption treatment on a polyethersulfone hollow fiber ultrafiltration base membrane through a polyethyleneimine aqueous solution to form a reactive anchoring layer; then sequentially dipping in an aqueous phase solution containing piperazine, sodium phytate and nanoscale cellulose whiskers and an organic phase solution containing trimesoyl chloride and 5-isocyanate isopeptide acyl chloride for interfacial polymerization; and finally, carrying out heat treatment on the obtained primary composite membrane, and completing a gas-phase cross-linking reaction in a glutaraldehyde steam atmosphere. According to the method, the hollow fiber nanofiltration membrane with high flux, high selectivity, excellent chlorine resistance and pollution resistance is successfully prepared through a multi-stage strengthening process, and the hollow fiber nanofiltration membrane is suitable for the fields of drinking water purification, industrial wastewater reuse, material separation and the like.

The invention discloses a PVA / urea / CC lignocellulose composite mulching film as well as a preparation method and application thereof, and belongs to the technical field of agricultural mulching films. The composite mulching film disclosed by the invention is obtained by adding PVA (Polyvinyl Alcohol) into a corncob lignocellulose HTP-CC dispersion liquid subjected to hydrothermal pretreatment, then sequentially adding glutaraldehyde, urea and glycerol and carrying out cross-linking reaction. The mechanical property of the PUCH mulching film prepared by the invention meets the GB / T35795-2017 standard, and the PUCH mulching film has excellent water vapor barrier property, ultraviolet shielding property, urea controlled release property, heat insulation property and biodegradability. The characteristics not only can promote crop growth, but also can reduce agricultural environmental pollution. Therefore, the PUCH mulching film shows huge potential to replace the traditional plastic mulching film, and has important application prospects in agricultural planting.

A technology for preparing glutaraldehyde through cyclopentene oxidation belongs to the technical field of organic synthesis, glutaraldehyde is prepared through cyclopentene oxidation by adopting a photocatalysis-electrocatalysis synergistic catalyst g-C3N4 / TiO2 (at) Au-Co / N-G. The catalyst is of a unique core-shell structure, is stable in property, promotes an oxygen reduction reaction in a catalysis technology, and has good catalytic activity. The conversion rate of cyclopentene and the selectivity of glutaraldehyde are remarkably improved, and the effect is remarkable.

The invention belongs to the technical field of aerogel, and discloses MXene-based aerogel and a normal-pressure preparation method and application thereof.The preparation method comprises the steps that mixed dispersion liquid of chitosan and conductive filler is mixed with glutaraldehyde, standing and crosslinking are conducted, and hydrogel is obtained; in the hydrogel, the conductive filler accounts for 20-75% of the mass of a solid substance, the chitosan accounts for 15-55% of the mass of the solid substance, and the glutaraldehyde accounts for 1-20% of the mass of the solid substance; the filler is selected from MXene, a carbon nano tube, graphene, boron nitride or MOF (Metal Organic Framework); the hydrogel is frozen at the temperature ranging from-20 DEG C to-80 DEG C; and carrying out normal pressure drying on the frozen hydrogel at 25-80 DEG C to obtain the aerogel. The solvent exchange-free normal-pressure drying preparation process breaks through the limitation of solvent exchange on the size, the high cost of a large amount of solvent is saved, large-area extensible preparation can be realized on the basis of energy conservation and environmental protection, and meanwhile, the prepared aerogel has relatively high mechanical strength, and the application range is widened.

The invention relates to the field of material chemistry on-line detection, in particular to a 1, 2, 4-trichlorobenzene on-line detection material and a preparation method thereof, and provides a film-forming sensitive layer material capable of being coated on the surface of a transducer, and the film-forming sensitive layer material comprises chitosan, a crosslinking component with the content of 0.1-5wt% in terms of glutaraldehyde and magnetic core-shell molecularly imprinted particles after being dried, the magnetic core-shell molecularly imprinted particle contains a ferroferric oxide magnetic inner core, a silicon dioxide middle layer and a molecularly imprinted polymer shell layer, and the shell layer is a cyclodextrin methacrylate and ethylene glycol dimethacrylate copolymer network and takes 1, 2, 4-trichlorobenzene as a template. The material keeps the membrane integrity and long-term stability under the working conditions of circulation and regeneration and also gives consideration to quick response, and the residual 1, 2, 4-trichlorobenzene is controlled to be not greater than 0.10 wt% and the residual glutaraldehyde is controlled to be not greater than 0.5 wt%. The method is used for relieving structural mass transfer contradiction and processing and coating coupling contradiction caused by high filler load, and has online detection application value.

The invention discloses green leaf-imitated porous hydrogel as well as a preparation method and application thereof, and belongs to the technical field of hydrogel materials. The preparation method of the green leaf-imitated porous hydrogel comprises the following steps: mixing polyvinyl alcohol, tannic acid and a composite pigment in a solid state, and grinding to form uniformly dispersed mixed powder; then spraying a glutaraldehyde solution to the mixed powder, carrying out a cross-linking reaction at room temperature, and washing after the reaction to obtain hydrogel; soaking the hydrogel in a lithium chloride solution, and taking out the hydrogel after soaking to obtain the green leaf-imitated hydrogel with the automatic moisture absorption function; the hydrogel has a porous structure, can automatically absorb moisture and simulate a vegetation spectrum in a 400-2500nm wave band at high precision, is suitable for covering the surface of fabric or equipment so as to simulate the spectrum and thermal characteristics of vegetation, and can be widely applied to the field of infrared camouflage.

The invention belongs to the technical field of water treatment, and particularly relates to a preparation method and application of a PDA / CS composite adsorbent loaded with a bimetallic system, and the preparation method comprises the following steps: 1) dissolving dopamine in a Tris buffer solution to prepare a dopamine prepolymer solution; then adding FeCl3, and carrying out constant-temperature water bath; centrifuging the obtained black suspension, performing suction filtration, washing, collecting a filter cake, and performing freeze drying to obtain PDA microspheres; 2) dispersing PDA microspheres in deionized water, and carrying out ultrasonic treatment until the PDA microspheres are uniformly dispersed; 3) dissolving chitosan in an acetic acid solution, and adding CuCl2 to prepare a mixed solution; the suspension obtained in the step 2 is dropwise added into a mixed solution for constant-temperature water bath, the pH of the mixed solution is adjusted through a NaOH solution, glutaraldehyde is added for constant-temperature water bath after constant-temperature water bath, and 4, the obtained suspension is subjected to centrifugation, suction filtration, washing, filter cake collection and freeze drying.

The invention belongs to the technical field of temporary plugging agents, and relates to a medium-temperature drilling fluid nano temporary plugging agent and a preparation method thereof. The temporary plugging agent is prepared from the following raw materials in parts by mass: 10 to 15 parts of small-particle-size coated silicon dioxide, 15 to 20 parts of medium-particle-size coated silicon dioxide, 30 to 50 parts of large-particle-size coated silicon dioxide, 15 to 20 parts of fiber, 5 to 8 parts of polyether diglycidyl ether, 5 to 8 parts of hyperbranched polyethyleneimine, 1 to 1.5 parts of water-soluble cellulose and 0.5 to 1 part of cationic quaternary ammonium salt. The coated silicon dioxide takes chitosan and glutaraldehyde as wall raw materials and silicon dioxide as a core material, and the particle size of the small-particle-size core material is 0.3-1 mu m; the particle size of the medium-particle-size particle core material is 150-250 microns; the particle size of the large-particle-size particle core material is 250-350 microns, the coated silicon dioxide particles are bonded together through a microscopic interpenetrating network formed by the reaction of hyperbranched polyethyleneimine and polyether diglycidyl ether, and the particles are matched with fibers to improve the bearing strength.

The invention discloses a super-hydrophobic chitosan aerogel PDMS (at) Co-PBA / CS and an application of the super-hydrophobic chitosan aerogel PDMS (at) Co-PBA / CS in oil-water separation. Adding Co-PBA into a chitosan aqueous solution, and then adding PVA; adding acetic acid and glutaraldehyde into the obtained mixed solution, stirring in a water bath, and freeze-drying to obtain Co-PBA / CS aerogel; and dissolving a hydrophobic material and a curing agent in an organic solvent, stirring to obtain a uniform suspension, immersing the Co-PBA / CS aerogel into the suspension, and carrying out vacuum drying to obtain PDMS-coated Co-PBA / CS. The super-hydrophobic chitosan aerogel PDMS (at) Co-PBA / CS prepared by the preparation method disclosed by the invention has relatively high adsorption capacity on both organic solvents and oil, and the removal efficiency is close to 100%. In addition, the composite material also has excellent self-cleaning and photo-thermal conversion performance, and can realize surface cleaning and removal of high-viscosity oil. The multifunctional aerogel provides a new strategy for removing pollutants such as oil in a complex and severe water environment.

The invention provides an adsorption material and a preparation method and application thereof, the adsorption material comprises a carrier and an endotoxin affinity binding ligand, the endotoxin affinity binding ligand comprises a recombinant C factor protein, and the carrier and the recombinant C factor protein are connected through a spacer arm; wherein the carrier comprises at least one of resin, chitosan, cellulose, agarose and glucan; the spacer arm comprises at least one of modified polyethylene glycol, polyvinyl pyrrolidone, polymethylacrylic acid-2-hydroxyethyl ester, polyvinyl alcohol, polyacrylic acid, hexamethylenediamine, divinyl sulfone and glutaraldehyde, and at least one end of the modified polyethylene glycol is provided with carboxyl, aldehyde or amino. The adsorption material provided by the invention can efficiently adsorb endotoxin, has the characteristics of low toxicity risk and excellent blood compatibility, and can improve the safety of blood purification.

This invention belongs to the field of silica technology, specifically relating to a method for preparing silica composite materials: silica microspheres or a silica mixture are added to a chitosan solution, followed by the addition of a PEI aqueous solution and stirring. A glutaraldehyde solution is then added, and the mixture is stirred again to obtain a gel-like product, which is then dried to obtain the silica composite material. The silica mixture includes a silica sponge-skeletal mixture, which is prepared by adding sponge-skeletal needles to a silica sol. This invention simplifies the preparation process of silica composite materials and improves the synergistic effect between silica and the matrix.

The invention relates to a chitosan-collagen synergistic self-assembly fiber material and a preparation method thereof, and belongs to the technical field of biological fiber materials. According to the method, the mass ratio of chitosan to collagen is controlled, co-assembly is induced through hydrogen bonds and electrostatic interaction in a low-temperature PBS solution, and then glutaraldehyde crosslinking, gradient dehydration and supercritical drying are performed to prepare the freeze-dried fiber. The obtained material completely retains the triple helix conformation of collagen, and has excellent conformation stability and enzymolysis resistance. The mechanical strength of the composite fiber is remarkably improved, and the composite fiber has excellent antibacterial property and cell affinity. The process does not need a toxic cross-linking agent, the operation is simple and convenient, and the prepared fiber is suitable for the high-end biomedical fields of tissue engineering scaffolds, intelligent antibacterial dressings, degradable nerve conduits and the like.

The invention discloses a bionic self-crimping material and application in the nerve field, and relates to the technical field of nano materials. The preparation method comprises the following steps: firstly, carrying out ultraviolet photopolymerization on acrylic acid and acrylic acid-N-hydroxysuccinimide, adding chitosan and glutaraldehyde to form hydrogel, and introducing epigallocatechin gallate into a chitosan-acrylic acid network. The material is rapidly and autonomously curled into a tube shape after being in contact with wet nerves, glutaraldehyde is directionally and orderly diffused by adopting a three-channel micro-fluidic chip, and the fineness is improved, so that the matching degree of nerve broken ends with different thicknesses is increased; the surface active groups of the material and the natural component chitosan cooperate to realize suture-free fixation; the plant phenol antioxidant removes free radicals at nerve injury parts; the degradation period is synchronous with natural healing of nerves, and secondary operation for taking out is not needed. The electroneurographic signals using the material are transmitted more smoothly, the target muscle atrophy degree is reduced, and the limb movement function is recovered to be close to the normal level.

The invention relates to a salt-resistant biomass interface evaporation material as well as a preparation method and application thereof, and relates to the technical field of interface evaporation materials. The preparation method comprises the following steps: mixing sunflower fiber dispersion liquid, polyvinyl alcohol and carbon-containing molybdenum disulfide, then adding glutaraldehyde, then carrying out liquid nitrogen freezing and freeze drying, sequentially soaking with a tannic acid solution, a calcium chloride solution and a ferric chloride solution, and then carrying out freeze drying. Natural sunflower fibers are used as a main framework, cotton is used as a supporting material, carbon-containing MoS2 is prepared and crosslinked with polyvinyl alcohol, a hydrophilic functional layer is constructed on the surface of the material through tannic acid, and the tannic acid is coordinated and complexed with Ca and Fe < + >, so that a more stable hydrophilic complexed coating is formed on the surface of the material; the surface wettability and the water conveying capacity of the material are further improved, meanwhile, salt crystal deposition and precipitation in the seawater evaporation process are effectively inhibited, and the oxidation resistance and the seawater corrosion resistance of the material are improved.

The invention discloses a paper-based diaphragm for alkaline water electrolysis and a preparation method of the paper-based diaphragm. A PPS / aramid fiber porous base material is prepared by adopting a wet non-woven process, a PVA-polymer quaternary ammonium salt blending layer is constructed on one side of the base material, OH <-> conduction is promoted by utilizing a quaternary ammonium group of polymer quaternary ammonium salt, and a three-dimensional network is formed through glutaraldehyde crosslinking. According to the paper-based diaphragm prepared through the method, when the porosity is 60.4%, the strength still reaches 8.98 MPa, the surface resistance of the diaphragm is greatly reduced to 68 m omega.cm < 2 >, the ionic conductivity synchronously jumps to 515 mS.cm <-1 >, the electrolytic current density is promoted to reach 1474 mA under the polarization voltage of 2.0 V, the vertical swelling rate of the diaphragm is stabilized at 12% through the cross-linked functional layer, and the paper-based diaphragm has the advantages that the paper-based diaphragm can be applied to the field of lithium ion batteries. The diaphragm achieves an optimal balance in key indexes such as aperture, strength, ionic conductivity and alkali liquor stability.

The invention belongs to the technical field of novel material catalysis, and particularly relates to a hollow mesoporous silica supported phosphotungstic acid catalyst and a preparation method and application thereof. According to the invention, the advantages of high specific surface area and regional effect performance of hollow mesoporous silica and the characteristic of adjustable acidity of vacancy heteropolyacid quaternary ammonium salt are innovatively combined, and the cationic surfactant is loaded on the surface of the hollow mesoporous silica, so that the contact surface of raw materials is effectively increased, efficient conversion of reactants is assisted, and the reaction efficiency is improved. The supported catalyst with efficient catalysis and stable performance is obtained. The catalyst can be used for a reaction for preparing glutaraldehyde through heterogeneous catalysis of cyclopentene oxidation, and the reaction condition is mild. Experiments show that under the catalytic system, the cyclopentene conversion rate can reach 90% or above, the glutaraldehyde selectivity is improved to 75% or above, double improvement of the cyclopentene conversion rate and the glutaraldehyde selectivity is successfully achieved, meanwhile, the advantage that the catalyst is easy to separate is achieved, and an efficient solution is provided for industrial application of synthesis of glutaraldehyde through cyclopentene oxidation.

The invention discloses a microneedle system for continuous blood glucose monitoring, which comprises a microneedle array, an electrode system, a circuit connection and signal acquisition unit and a fixing and packaging structure, the working electrode is used for penetrating into the skin and making contact with intertissue fluid to control the diffusion speed of glucose and prevent glucose oxidase from losing, the surface of the working electrode is wrapped with glucose oxidase cross-linked and fixed through glutaraldehyde, one end of the electrode system is inserted into the microneedle, and the other end of the electrode system is connected with the circuit connection and signal acquisition unit. The fixing and packaging structure is arranged on the outer side of the circuit connecting and signal collecting unit and used for wrapping the electronic device and fixing the microneedle system to the skin surface. According to the microneedle system for continuous blood glucose monitoring, rapid and accurate detection of in-vivo blood glucose level changes is achieved, and minimally invasive property and good patient compliance can be achieved while the detection sensitivity and accuracy are guaranteed.

The invention discloses a preparation method and application of a catalyst with a high-valence tungsten coordination structure, and the preparation method of the catalyst comprises the following steps: enabling tungstic acid and hydrogen peroxide with specific concentration and proportion to react at a certain temperature to prepare a peroxy tungstic acid solution; dissolving a polydentate organic ligand capable of forming a stable chelation structure with a tungsten center in an organic solvent, mixing the obtained ligand solution with a peroxytungstic acid solution according to a specific ratio, and carrying out a coordination reaction at a certain temperature until the ligand is completely reacted; and finally, carrying out solid-liquid separation, washing and drying to obtain the high-valence tungsten coordination structure catalyst. The catalyst can be used for a reaction for synthesizing glutaraldehyde through cyclopentene oxidation, under the mild condition, hydrogen peroxide serves as a green oxidizing agent, the conversion rate of cyclopentene reaches 90% or above, the selectivity of glutaraldehyde exceeds 60%, the catalyst is stable in structure and can be recycled for 10 times or above, and the problems that in the prior art, a catalyst is difficult to separate, low in activity, poor in stability and the like are solved; the method has a remarkable industrial application value.

The invention relates to the technical field of fibers, and particularly discloses a skin-friendly fiber, a preparation method of the skin-friendly fiber and a hygienic product with the skin-friendly fiber. The skin-friendly fiber comprises a fiber matrix and a coating layer coating the fiber matrix, and the fiber matrix comprises a core layer and a skin layer coating the core layer; the core layer is prepared from the following raw materials: PET resin, whitening master batch, a flexibilizer, a compatilizer and an antioxidant; the skin layer comprises the following raw materials: PE resin, an initiator, glycidyl methacrylate and tertiary amine; the coating layer comprises the following raw materials: red camellia oil microcapsules and polylysine; the capsule core of the red camellia oil microcapsule is red camellia oil, and the capsule wall of the red camellia oil microcapsule is prepared from the following raw materials: chitosan, hyaluronic acid and a glutaraldehyde solution in a mass ratio of 1: (2-3): (1.5-2). The skin-friendly fiber can be used for manufacturing non-woven fabrics of hygienic products, and has the advantages of being skin-friendly, soft, breathable and high in moisture penetrability.

The invention relates to the technical field of immunochromatography, in particular to time-resolved fluorescent microspheres, a preparation method and application of the time-resolved fluorescent microspheres in PSP / CRP / MxA quantitative detection immunochromatography test strip, and the preparation method of the time-resolved fluorescent microspheres comprises the following steps: 1) preparing surface aminated polystyrene microspheres; (2) carrying out glutaraldehyde crosslinking and 1, 10-phenanthroline-5-amino combination; 3) grafting a rare earth fluorescent dye on the surface; and 4) coating a polymer shell layer and modifying carboxyl. According to the invention, through a dual fixation strategy of surface grafting and polymer coating, the time-resolved fluorescent dye is confined to a functional region between the surface of the microsphere and a polymer shell layer, diffusion and leakage of dye molecules to the external environment are blocked, and the problem of dye shedding of traditional fluorescent microspheres is solved; the polymer shell layer can effectively isolate external ultraviolet light, oxygen and other corrosive factors, reduce photooxidative degradation and non-radiative transition loss of fluorescent molecules, and improve the light stability and fluorescent lifetime of the fluorescent molecules.

The invention relates to the field of biomimetic materials, in particular to a biomimetic self-cleaning anti-fouling aquaculture net cage material, a preparation method and application of the biomimetic self-cleaning anti-fouling aquaculture net cage material. 0.5 to 2.0 parts by weight of a carboxylated graphene nanosheet; 2.0 to 5.0 parts by weight of nitrogen-doped titanium dioxide nanoparticles; 1.0 to 3.0 parts by weight of silica nanoparticles; 5.0 to 10.0 parts by weight of calcium ion cross-linked modified sodium alginate; 1.0 to 3.0 parts by weight of octadecyl trichlorosilane; 0.5 to 1.5 parts by weight of lauryl sodium sulfate; through the synergistic effect of a double bionic structure and enhanced photocatalysis, the effective inhibition on attachment of various organisms is realized, and the inhibition rate on attachment organisms such as algae, bacteria and barnacles reaches 75% or above.

The invention discloses a high-barrier anti-permeation packaging bag and a processing method thereof, and relates to the technical field of packaging bag preparation. Comprising the following raw materials in parts by mass: 150 to 200 parts of deionized water, 50 to 70 parts of polyvinyl alcohol, 3 to 5 parts of modified montmorillonite, 7.5 to 10.5 parts of carboxylated cellulose nanofiber, 3 to 8 parts of zinc-doped mesoporous silica, 3 to 5 parts of a synergist, 5 to 10 parts of glycerol and 0.25 to 0.35 part of glutaraldehyde. By introducing the synergist prepared from sodium chloroacetate, triethylamine, calcium chloride dehydrate and the like, the water vapor and oxygen barrier capacity and the antibacterial capacity of the packaging bag can be effectively improved; and due to the introduction of the modified montmorillonite, the carboxylated cellulose nanofiber, the zinc-doped mesoporous silica and other components, the water vapor and oxygen barrier ability and the antibacterial ability of the packaging bag are further improved. Therefore, the method has a wider application prospect.

The invention discloses a method for extracting edible collagen and preparing a collagen casing, which comprises the following steps of: mixing collagen dispersion or collagen slurry formed by collagen blocks with soy isolate protein to form a composite collagen casing; the preparation method comprises the following steps: mixing collagen dispersoid or collagen pulp and soybean protein isolate in a solvent according to a mass ratio, uniformly stirring, adding glycerol, uniformly stirring and mixing, adding glutaraldehyde, continuously stirring for about 10-30 minutes to form a film-forming solution, uniformly stirring and mixing, finally putting the film-forming solution into an air dry oven, drying, taking out, and putting into a constant-humidity dryer for 48 hours for later use. The tubular casing subjected to extrusion molding is solidified to be shaped, the extruded casing passes through a coagulating bath, the molecular structure of collagen is changed, and a flowable sol state is converted into a stable gel state, so that the casing obtains strength and toughness, and ammonia water or an alkaline salt solution is put into the bath.

The invention relates to the technical field of bioengineering, in particular to a process for continuously producing nicotine based on immobilized enzyme. The immobilized microbial agent comprises a composite carrier, an engineering strain and an immobilized auxiliary agent, in the process for continuously producing nicotine based on immobilized enzyme, amine oxidase of an engineering strain is subjected to site-specific modification, so that spatial structure deformation when the amine oxidase is combined with a carrier is reduced, and a three-dimensional network structure of hydroxyethyl chitosan-guar gum composite microspheres in the composite carrier is matched to provide an adaptive microenvironment; the activity loss of enzyme molecules caused by steric hindrance or structural change is reduced; in the immobilization auxiliary agent, gallic acid and an enzyme molecule sulfydryl form a coordinate bond to maintain an active center conformation, glutaraldehyde and polyethyleneimine enhance the binding stability through a crosslinking effect and charge regulation, and the maintaining effect of enzyme activity during continuous production is improved, so that the enzyme activity loss in the immobilization process is reduced, and the immobilization efficiency is improved. The stability of the enzyme in long-term continuous reaction is enhanced.

The invention relates to the technical field of sewage treatment, in particular to a biological enzyme-containing deodorizing and antibacterial composition for sewage treatment. The biological enzyme-containing deodorizing and antibacterial composition for sewage treatment is prepared from the following raw materials in parts by mass: 1 to 3 parts of a compound biological enzyme preparation, 5 to 15 parts of bamboo charcoal powder, 1 to 2 parts of chitosan, 1 to 3 parts of dopamine, 0.005 to 0.024 part of glutaraldehyde, 10 to 30 parts of bentonite, 1 to 5 parts of graphene, 1 to 3 parts of nano silicon dioxide, 10 to 20 parts of wood flour, 5 to 10 parts of sodium hydrogen sulfite and 1 to 5 parts of sodium carboxymethyl cellulose. 1-2 parts of polyacrylamide and 1-5 parts of a nonionic surfactant. The composition disclosed by the invention forms a synergistic effect, can remove most suspended matters and metal ions in sewage, can quickly catalyze and decompose organic matters into water-soluble small molecular compounds such as organic acid and alcohol, efficiently and remarkably reduces the contents of nitrogen and phosphorus in the sewage and purifies a water body.

The invention belongs to the technical field of enzyme engineering and gene engineering, and particularly relates to a recombinant immobilized enzyme, an expression gene thereof and application of the recombinant immobilized enzyme in production of macrocyclodextrin. According to the preparation method, SpyTag and SpyCatcher peptides are fused to the N end of isoamylase and the N end of 4-alpha-glucanotransferase respectively, the 4-alpha-glucanotransferase modified by the SpyTag and the isoamylase modified by the SpyCatcher are obtained, and after spontaneous isopeptide bonds are formed between the SpyTag and the SpyCatcher, the self-assembled macrocyclodextrin production enzyme cluster is obtained. The obtained macrocyclodextrin production enzyme cluster forms an enzyme aggregate with a three-dimensional structure under the traditional glutaraldehyde-mediated catalytic action, namely the recombinant immobilized enzyme. The recombinant immobilized enzyme disclosed by the invention can be repeatedly utilized, is simple and convenient to operate, is easy to separate a converted product, and can be applied to industrial production of the macrocyclodextrin.

The application discloses a preparation method and application of a POMs-PEI nanofiltration membrane and belongs to the nanofiltration membrane preparation and application field. 12 The preparation method of the nanofiltration membrane is as follows: step 1, performing first soaking treatment on a PAN membrane in a PMo 12 solution to obtain a POMs modified membrane; and step 2, performing second soaking treatment on the POMs modified membrane in a PEI solution and then performing third soaking treatment on the POMs modified membrane in a glutaraldehyde solution to obtain the POMs-PEI nanofiltration membrane. The application solves the problem of low dye and salt ion separation efficiency of traditional polymer membrane treatment industrial printing and dyeing wastewater, the prepared nanofiltration membrane has excellent dye and salt separation performance and chemical stability, the preparation process is simple, and the nanofiltration membrane is suitable for large-scale industrial production.