68 results about "O carboxymethyl chitosan" patented technology

The invention discloses a folic acid-modified O-carboxymethyl chitosan-deoxycholic acid complex which is an active-targeting nano-micelle carrier material prepared by using folic acid, O-carboxymethyl chitosan and deoxycholic acid as raw materials; O-carboxymethyl chitosan is used as a carrier material; deoxycholic acid is used for hydrophobicity reconstruction; folic acid is used for surface modification; and folic acid-mediated tumor tissue-targeting polymer micelle is prepared. Drug-loaded nano-micelle is prepared by a self-assembly method with paclitaxel as a model drug; experiment demonstrates that the drug-loaded nano-micelle has high drug load and encapsulation efficiency, has sustained release effect, is intaken into tumor cells through a folic acid acceptor approach, can increase the distribution of the drug in tumor tissue, thus improves curative effect, reduces toxic and side effect, and reaches the purpose of targeting treatment. The invention provides an ideal and novel drug carrier and preparation form for hard-soluble antitumor drugs.

The invention belongs to a marine chemical engineering technology, and concretely relates to a marine organism polysaccharide Schiff base derivative, a preparation method thereof, and an application thereof as an agricultural bactericide. The polysaccharide Schiff base derivative is an O-carboxymethyl chitosan aminobenzoic acid Schiff base derivative, and is represented by general formula I; and in the formula I, X is a carboxyl and methyl co-substituted phenyl group, a carboxyl and halogen co-substituted phenyl group or a carboxylphenyl group, and n is 1000-8000. The O-carboxymethyl chitosan aminobenzoic acid Schiff base derivative has good dissolvability and can be dissolved in various solvents, so the application field of the derivative is enlarged, the derivative has bacteriostatic activity and metal ion adsorption function, can kill agricultural pathogenic bacteria, and also can remove copper ions and other heavy metal ions enriched in cultivated land. The derivative has potential application values in the field of pesticides.

The invention belongs to the field of medical materials, particularly relates to N,O-carboxymethyl chitosan-polyaldehyde hyaluronic acid gel and use thereof, and aims at providing an anti-adhesion blocking agent which is good in anti-adhesion effect, good in biocompatibility and bioabsorbable. The N,O-carboxymethyl chitosan-aldehyde hyaluronic acid gel provided by the invention has structural unit shown in a formula I described in the specification, wherein x=10-10,000 and y=10-10,000. The invention also provides a preparation method of the N,O-carboxymethyl chitosan-aldehyde hyaluronic acid gel, and use thereof in prevention and treatment of postoperative tissue adhesion. The prepared N,O-carboxymethyl chitosan-aldehyde hyaluronic acid gel provides a novel choice for the anti-adhesion blocking agent.

The invention discloses a composite long-acting formaldehyde remover, comprising the following components by weight percent: 5-10% of 6-O-carboxymethyl chitosan and 5-10% of urea, the balance of water, and the sum of the components is 100%. Deacetylated degree of the 6-O-carboxymethyl chitosan is more than 90%, substitution degree is 60-70%, and weight-average molecular weight is 20000-500000. The components are weighed by the formula, the 6-O-carboxymethyl chitosan and urea are respectively added with water to be dissolved, and then the two solutions are mixed and compounded by the volume ratio of 1:1, thus obtaining the remover. The composite long-acting formaldehyde remover is convenient to use, the remover is coated on the surface of objects capable of releasing formaldehyde such as wall, furniture and textile, formaldehyde released by furniture, floor board and wall paper in house decoration can be effectively removed, no dead angle is kept, the remover is long-term effective, and no secondary pollution is produced, thus ensuring that human settlement is not puzzled or threatened by formaldehyde any longer.

The invention discloses a synthesis method of a 6-O-carboxymethyl chitosan sulfation product. The method comprises the following steps: (1) chitin alkaline treatment of chitin; (2) carboxymethylation of C6-O site of chitin; (3) deacetylation reaction of 6-O-carboxymethyl chitin; and (4) sulfation of 6-O-carboxymethyl chitosan. The synthesis method disclosed by the invention is a bran-new method for selectively replacing and controlling the replacement rate by using chitin. The synthesis product of the method provides N-site -SO3H with main anticoagulant activity and introduces -COOH, so that a lot of -COOH and SO3H which have negative electricity in the molecular structure are regularly distributed, and an anticoagulant effect of heparinoid is generated by the synergistic effect. High molecular polysaccharide is a heparinoid drug which is selectively modified by chitin via a safe reagent, so that reagent pollution of bulk drugs is reduced, the virus contamination risk of heparin biological extraction is avoided, the safety performance of the drug in the clinical experiment is more excellent in theory as compared with heparin sodium, so the 6-O-carboxymethyl chitosan sulfation product provided by the invention is expected to serve as a cheap direct thrombin inhibitor to replace heparin sodium anti coagulation drugs.

The invention belongs to marine chemical engineering technologies, and particularly relates to a marine organism polysaccharide copper compound, preparation thereof and application of the marine organic polysaccharide copper compound serving as agricultural bactericide. The marine organism polysaccharide copper compound is an O-carboxymethyl chitosan diacetone aminobenzoic acid Schiff base copper compound derivative, and the general formula of the compound is shown as the formula I, in the formula I, X is phenyl for replacing carboxyl and methyl at the same time or phenyl or carboxyl phenyl for replacing carboxyl and halogen at the same time, and n=1000-8000. The prepared O-carboxymethyl chitosan diacetone aminobenzoic acid Schiff base copper compound derivative has good solubility, can be dissolved in kinds of solvent, and avoids heavy metal ion residues in soil while improving the antibacterial effect, the application field of the compound is widened, and the compound has potential application value in the field of pesticide.

The invention discloses a method for preparing a moisturizing agent N-maleylation-O-carboxymethyl chitosan, which is characterized by comprising the following steps: weighing 2 grams of O-carboxymethyl chitosan, putting the O-carboxymethyl chitosan in a reactor, adding 90 milliliters of acetic acid solution of which volume fraction is 3 percent into the reactor, stirring the mixture to dissolve for 1 hour till forming homogeneous phase solution, weighing maleic anhydride in a molar ratio of 1 to 1 with the O-carboxymethyl chitosan again, then dissolving the maleic anhydride in 5 milliliters of acetone, adding the solution into the reactor by stirring, and reacting the mixture for 15 hours at room temperature; and purifying the product by using the acetone, and drying the purified product in a vacuum constant temperature drying oven at the temperature of 50 DEG C to obtain N-maleylation-O-carboxymethyl chitosan. The method has the advantages that the moisturizing agent N-maleylation-O-carboxymethyl chitosan has better safety and bioactivity compared with other moisturizing agents and has broad market demands.

A gluconic acid modified chitosan nucleophilic NO donor and synthesizing method belongs to the medicine engineering technical field. The invention modifies the carboxylation reaction to NH2 group on chitosan or O-carboxymethyl chitosan for leading the NH2 group to produce nucleophilic NH group which can react with NO. Secondary amine NH group on gluconic acid modified chitosan or O-carboxymethyl chitosan molecules carries out reaction in a methanol solution of sodium methoxide with NO gas molecules, wherein, Na⁺/NH=2, the [N(O)NO]^- group is produced, and the molecular structural formula of the achieved gluconic acid modified chitosan or O-carboxymethyl chitosan nucleophilic NO donor is as follows.s The nucleophilic NO donor of the invention has different NO release speeds and larger load capacity, can simultaneously overcome cytotoxicity of nucleophilic reagent (polyamines) and avoid from producing oncogenicity coproduct of nitrosamine, and has a certain targeting capacity. R=H, CH2COONa.

The invention belongs to the technical field of crop anti-freezing, and discloses an anti-freezing agent for crops.The anti-freezing agent is prepared from, by mass, 15-20 parts of polyethylene glycol, 10-15 parts of N,O-carboxymethyl chitosan, 1-2 parts of soybean hydrolyzed amino acid, 1-3 parts of calcium lactate, 1-3 parts of monopotassium phosphate, 0.01-0.03 part of 6-benzylamino adenine, 0.2-0.4 part of brassin, 0.02-0.04 part of diethyl aminoethyl hexanoate, 0.01-0.03 part of abscisic acid and 1000 parts of distilled water.The anti-freezing agent is good in anti-freezing effect and cannot cause excessive growth of plants.

The present invention discloses a method for preparing a shoe antibacterial synthesis leather substrate and a liner through an O-carboxymethyl chitosan grafted nylon 66 fabric. The method comprises: 1) partially hydrolyzing a nylon 66 fabric; 2) carrying out carboxyl protecting and amino protecting ; 3) preparing O-carboxymethyl chitosan; 4) carrying out amino protecting on the O-carboxymethyl chitosan; 5) carrying out an amide condensation reaction on the O-carboxymethyl chitosan and the nylon 66 fabric obtained in the step 2); and 6) removing the amino protecting agent and the carboxyl protecting agent, and washing to obtain the O-carboxymethyl chitosan/nylon 66 shoe antibacterial synthesis leather substrate and the liner. According to the present invention, the chitosan and the nylon 66 base cloth are adopted as the raw materials and the grafting method is used to synthesize the O-carboxymethyl chitosan/nylon 66 shoe synthesis leather substrate and the liner, and the grafted and modified nylon 66 shoe synthesis leather substrate and liner have characteristics of a certain water vapor permeability and good hygiene performance.

The invention discloses a method for preparing O-carboxymethyl chitosan covered nano-silver. According to the method, after silver nitrate solutions and O-carboxymethyl chitosan solutions are mixed according to a ratio, O-carboxymethyl chitosan covered nano-silver antibacterial agents are prepared through ultraviolet irradiation; the nano-silver serves as a main antibacterial ingredient, O-carboxymethyl chitosan serve as an auxiliary antibacterial ingredient, a reducing agent and a stabilizer, the toxicity of the antibacterial agents is lowered, Ag ions are reduced to elemental silver by the O-carboxymethyl chitosan on the condition of ultraviolet irradiation, and the O-carboxymethyl chitosan is adsorbed on the surface of a silver core to prevent aggregation of the nano-silver and realizes the synergistic antibacterial effect; and during detection, the minimal inhibitory concentration of the antibacterial agents to escherichia coli is 1 micro/ milliliter and to staphylococcus aureus is 2 micro/ milliliter and reaches the minimum concentration limit of the best similar products at home and abroad, the used materials are harmless to the human body, the price is low, and therefore the antibacterial agents are friendly to environment.

The invention relates to a method for preparing O-carboxymethyl chitosan nanoparticles, which is characterized by comprising the following steps: dissolving 1.0g of chitosan in 20ml of acetic aqueous solution with a volume ratio of 1 percent, stirring to dissolve the chitosan, adding 20ml of isopropanol solution into dissolved chitosan colloid, stirring to fully and uniformly mix the mixture, soaking for 17 hours at room temperature, then adding 42 percent potassium hydroxide solution, and stirring to acquire white chitosan deposits in the shape of short fibers. The method has the advantages of mild reaction condition, simple production technology and easy industrialized production, and the acquired O-carboxymethyl chitosan nanoparticles have no toxicity or harm, and can be used for adsorbing toxic metal ions.

The invention relates to a method for preparing a chitosan encapsulated nano-silver graphene oxide composite antibacterial material. The method comprises the steps of proportionally mixing a silver nitrate solution and an O-carboxymethyl chitosan solution, then, carrying out ultraviolet irradiation so as to prepare an O-carboxymethyl chitosan encapsulated nano-silver antibacterial agent, and carrying out self-assembly on purified O-carboxymethyl chitosan encapsulated nano-silver particles and graphene oxide in an aqueous solution, thereby preparing the O-carboxymethyl chitosan encapsulated nano-silver graphene oxide composite antibacterial material. According to the method, O-carboxymethyl chitosan and the graphene oxide serve as an auxiliary antibacterial agent and a stabilizer, so that the re-adding of chemical reagents is avoided, and the toxicity of the antibacterial agent is lowered. Under ultraviolet irradiation, Ag ions are reduced to elemental silver, O-carboxymethyl is adsorbed to the surface of silver cores, and silver nanoparticles are adsorbed to the surface of the graphene oxide after self-assembly, so that a synergistic antibacterial action is exerted; and the used materials are harmless to human bodies and are low in price, so that the method is an environment-friendly novel composite antibacterial agent preparation method.

The invention belongs to the technology of ocean chemical engineering and particularly relates to a marine organism polysaccharide copper compound and a preparation method and application thereof.The compound is as shown in the formula I.In the formula I, X is pyridyl, and n is 4-4000.The compound shown in the formula I is applied to agricultural fungicide preparation.A prepared O-carboxymethyl chitosan acetylacetone nicotinamide schiff base copper compound has good solubility, can be dissolved in various solvents, improves the antifungal effect, prevents heavy metal ions from being left in soil and has potential application value in the pesticide field, and the application field of the compound is widened.The formula I is as shown in the description.

The invention provides a preparation method of O-carboxymethyl chitosan resin. The method comprises the following steps: 1) preparing single O-carboxymethyl chitosan by using chitin as a raw material, performing carboxymethylation under relatively high temperature by using chitin as the raw material, and then preparing single O-carboxymethyl chitosan with relatively good selectivity by removing acetamido on a bit C2; 2) preparing the O-carboxymethyl chitosan resin by adopting an emulsification crosslinking method, firstly protecting -NH2 and -COO<-> together by adopting a complex reaction between CuSO4 and -NH2 and -COO<-> of O-carboxymethyl chitosan and a Schiff base reaction between formaldehyde and -NH2 of O-carboxymethyl chitosan; then enabling part of hydroxyl on O-carboxymethyl chitosan molecules to participate a crosslinking reaction by using glutaric dialdehyde as a crosslinking agent, thus preparing the O-carboxymethyl chitosan resin with a net structure; finally washing off Cu<2+> and formaldehyde by using hydrochloric acid to obtain glutaric dialdehyde crosslinked O-carboxymethyl chitosan resin containing a great number of active groups. The prepared O-carboxymethyl chitosan resin provided by the invention contains amino groups and carboxyl groups, can be connected with various biological active substances, has relatively good adsorption effects on acid radical ions and protein, has strong load capacities on heavy metal ions, can be bio-degraded, is good in acid resistance and alkali resistance, and can be applied to the field of foods, medicines and chemical industries.

The invention discloses an anti-haze spray for improving the haze filtering capacity of a mask. The anti-haze spray is prepared from, by weight, 4-6 parts of N,O-carboxymethyl chitosan, 3-5 parts of sodium alginate, 2-4 parts of ethyl alcohol, 1-3 parts of copper sulfate, 80-100 parts of water and 4-6 parts of diethylenetriamine pentaacetic acid and glycerin, wherein the ratio of the weight part of diethylenetriamine pentaacetic acid to the weight part of glycerin is (3-5):1. The anti-haze spray further comprises lysozyme, and the concentration of lysozyme is 400,000-600,000 per gram, in other words, 400,000-600,000 pieces of lysozyme are contained per gram of the anti-haze spray. When the spray is sprayed, the filtering capacity of the common mask for PM2.5 can be remarkably improved. The anti-haze spray will not affect the breathability of the mask. The anti-haze effect of the anti-haze spray may be related with the ratio of the weight part of diethylenetriamine pentaacetic acid to the weight part of glycerin in the formula.

The disclosed preparation method for an electrode chemical modified by O-carboxylmethychitose covalence bond comprises: dipping glass-carbon electrode into O-carboxylmethychitose-formic acid solution for modification, taking out to clean with formic acid and distilled water. This invention can improve detection sensitivity for DA, can eliminate effect of AA and UA, and has long lifetime.

The invention discloses modified chitosan pakchoi concentrated fertilizer with high infectious microbe inhibiting activity. The modified chitosan pakchoi concentrated fertilizer with high infectious microbe inhibiting activity comprises the following components in parts by weight: chitosan cationic solution, cinnamic acid, cerium oxalate, humulone, algin, shrimp and crab shells, tremolite, iron-containing waste residue, nano carbon, EM microbial agent, zinc methionine complex, ethoxy quinoline, porous high-activity humic acid, superfine glass fibers with micropores, O-carboxymethyl chitosan and right amount of weakly acidic small molecular group activated water. The modified chitosan pakchoi concentrated fertilizer with high infectious microbe inhibiting activity adopts chitosan rare earth cerium copolymer carried by the superfine glass fibers, supplement of cerium oxalate and antibacterial cultivation on the pakchoi are realized, and the weakly acidic small molecular group activated water and O-carboxymethyl chitosan treated cinnamic acid are combined, so that antibacterial, disease-resistant and high-efficiency cultivation is promoted, and natural environment-friendly cultivation is realized; meanwhile, shrimp and crab shell waste, tremolite, sandy soil and iron-containing waste residue are combined for composting, and the fertilizer has good permeability, is rich in nutrients and can promote high-efficiency cultivation of the pokchoi.

The invention provides preparation and application of calcium sulphate cement with osteogenic activity and antibacterial property. The calcium sulphate cement comprises a solid phase and a liquid phase, wherein the solid phase comprises the following components in percentages by mass: 80-90% of calcium sulphate and 10-20% of silver-strontium hydroxyl apatite; and the liquid phase is 0.1-1wt% of an aqueous solution of O-carboxymethyl chitosan. The solid-phase calcium sulphate is modified by synthesized strontium-silver-doped hydroxyl apatite in a compounded manner, the doped strontium which is a microelement has double effects of inducing osteogenesis and suppressing osteoclast, the silver as an antibacterial agent can be used widely, and owing to addition of the strontium and the silver, the osteoconduction and the antibacterial property of the calcium sulphate cement are improved. In the liquid phase, O-carboxymethyl chitosan molecules contain abundant hydroxyls (-OH) and carboxyls (-COOH), the biological activity and the biological compatibility of the calcium sulphate cement are improved effectively, and the prepared calcium sulphate cement has the advantages of long injectable time, high compressive strength, small degradation rate and the like.

A preparation method of an N-2-hydroxypropyl trimethyl ammonium chloride chitosan/ N,O-carboxymethyl chitosan naonparticle relates to a preparation method of the chitosan naonparticle. The method comprises the following steps of: 1, taking an N-2-hydroxypropyl trimethyl ammonium chloride chitosan solution, dripping an N,O-carboxymethyl chitosan naonparticle solution therein, stirring magnetically at a room temperature and under a sterile condition, and obtaining a solution A; and 2, centrifugating the solution A, eluting the deposit with deionized water for three times and performing freeze drying under vacuum to obtain the N-2-hydroxypropyl trimethyl ammonium chloride chitosan/ N,O-carboxymethyl chitosan naonparticle. Compared with the chitosan naonparticle, the N-2-hydroxypropyl trimethyl ammonium chloride chitosan/ N,O-carboxymethyl chitosan naonparticle obtained by the method has the advantages that the particle diameters can be easily controlled and are uniformly distributed, the biological compatibility is good, the stability is high, and has the characteristics of good water solubility, electropositive property and the like; in the process of preparing the nanoparticle, the safety and the nontoxicity are achieved; the preparation process is simple; the operation is simple; the preparation conditions are mild; the cost is low; and the N-2-hydroxypropyl trimethyl ammonium chloride chitosan/ N,O-carboxymethyl chitosan naonparticle can be easily produced on a large scale.

The invention discloses a preparation method for N-2-hydroxypropyldimethylethyl ammonium chloride chitosan (N-2-HFCC)/carboxymethyl chitosan Newcastle disease virus nanoparticles, relates to preparation methods for virus nanoparticles, and solves problems that chitosan nanoparticles have low encapsulation rate and drug-loading capacity on vaccine antigens and poor adsorption capability on the mucosal surface. The method comprises the following steps: 1, preparation of N-2-HFCC; 2, preparation of the N-2-HFCC/carboxymethyl chitosan Newcastle disease virus nanoparticles, wherein a, a Newcastle disease L-series virus solution is dropwise added into an N-2-HFCC solution, stirring is performed, and a solution A is obtained; b, the solution A is stirred, an N,O-carboxymethyl chitosan solution is dropwise added, and a solution B is obtained; c, the solution B is centrifuged, precipitated, washed with water and dried to obtain the N-2-HFCC/N,O-carboxymethyl chitosan nanoparticles loaded with Newcastle disease attenuated live vaccines. The nanoparticles prepared with the method are high in biosafety, good in bacteriostatic effect and high in encapsulation rate. The method is used for preparing the Newcastle disease virus nanoparticles.

The invention relates to a nanoscale chitosan derivative ultrasonic contrast medium capable of converting surface charges and a preparation method of the contrast medium. According to the nanoscale chitosan derivative ultrasonic contrast medium, tween 20, lecithin and perfluorohexane are adopted for preparing a mixed suspension solution through a nano emulsion method, O-carboxymethyl chitosan is stirred and dispersed in the mixed suspension solution through a high-speed machine, and after low-speed centrifugation and filtering, the nanoscale chitosan derivative ultrasonic contrast medium, witha small and even particle size, capable of converting the surface charges is obtained. According to the prepared nanoscale chitosan derivative ultrasonic contrast medium capable of converting the surface charges, with the O-carboxymethyl chitosan as a shell membrane, a perfluorohexane solution is wrapped by the interior of the shell membrane, the particle size is 150-200 nanometers, the ultrasonic contrast medium can penetrate through the blood vessel endothelium and enter the tumor microenvironment and has targeting performance and high efficiency on tumor treatment, the ultrasonic contrastmedium and protein substances in the serum are not easily subjected to protein agglutination in the blood environment, and the ultrasonic contrast medium has good blood compatibility and stability andfurther has a good drug(doxorubicin)-loaded capacity.

The invention belongs to a marine chemical engineering technology and particularly relates to a marine biological polysaccharide-copper compound and its preparation method and application. The compound is shown in the formula I. The compound shown in the formula I is used for preparation of an agricultural fungicide. The O-carboxymethyl chitosan acetylacetone aminopyridine schiff base-copper compound has good solubility, is soluble in a variety of solvents, improves the antibacterial effects, prevents the heavy metal ion residues in the soil, expands an application field and has a potential application value in the field of pesticide. In the formula I, R represents hydrogen, methyl, chlorine or trifluoromethyl and n is 4 to 4000.