97results about How to "Enhance osteogenic activity" patented technology

The invention provides a porous metal implant with a micro-arc oxidation coating and a preparation method. The preparation method comprises the steps of bracket preparation and micro-arc oxidation, specifically, a porous metal bracket with pores partially or completely communicating with one another is prepared at first, and the size of each pore is 300-1800 [mu]m; and then, the porous metal bracket is subjected to micro-arc oxidation under the stirring condition, and the porous metal implant with the external surface and the internal surface of the bracket being each coated with the micro-arc oxidation coating is prepared. According to the porous metal implant with the micro-arc oxidation coating and the preparation method, the uniform micro-arc oxidation coating containing calcium and phosphorus is formed on the internal and the external surface of the porous metal, the osteogenic activity of the porous metal can be facilitated, bone ingrowth can be improved, and the bone integration capability of the porous metal is improved; in addition, the coating process is high in efficiency, low in cost, free of pollution and convenient to apply industrially.

The invention discloses a magnesium oxide whisker/biodegradable polyester composite material and its preparation method and an application thereof. The composite material contains 0.1-60 wt% of magnesium oxide whisker and 40-99.9 wt% of biodegradable polyester. The magnesium oxide whisker/biodegradable polyester composite material provided by the invention has excellent mechanical properties, good biocompatibility and bone healing-promoting capability. Alkalinity of the magnesium oxide whisker also can regulate and control degradation rate of the biodegradable polyester, neutralize its acidic degradation products and reduce inflammatory response. The magnesium oxide whisker/biodegradable polyester composite material is a novel bone tissue repair material with an excellent osteogenic activity. The preparation method is a solution blended process or a melt blending method by using the magnesium oxide whisker and the biodegradable polyester as raw materials, or an in situ polymerization method by using the magnesium oxide whisker and a cyclic ester monomer as raw materials. According to the invention, preparation cost of the composite material is low; the preparation method is simple; condition is mild; and the preparation method is easy for industrial production.

The invention provides a PEEK (polyether-ether-ketone) surface modification method. The method comprises steps as follows: PEEK is sulfonated with concentrated sulfuric acid, and sulfonated PEEK is obtained; sulfonated PEEK is subjected to reduction treatment with sodium borohydride at the temperature of 80-160 DEG C for 0.5 h or longer.

The invention discloses a 3D printed Ti-PDA-PLGA microsphere bone defect repair stent. A 3D printed Ti stent is prepared by a laser sintering technology. Then, under certain conditions, dopamine is self-polymerized on the fiber surface of the 3D printed Ti stent to form a PDA coating, thereby preparing a 3D printed Ti-PDA stent; then PLGA microspheres carrying VEGF are prepared by a double emulsion-solvent evaporation method, and finally, BMP-2 and PLGA microspheres carrying VEGF are adsorbed and immobilized on the surface of the stent by an adsorption method, and finally the 3D printed Ti-PDA-PLGA microsphere bone defect repair stent is formed. The bone defect repair tissue engineering stent disclosed by the invention has the advantages of reliable mechanical property, high biological activity and safety, convenient implantation, small trauma and low cost, and can be used for the repair treatment of bone defect after bone traumas, bone tumors and bone infection.

The invention relates to a preparation method of a porous titanium/hydroxyapatite composite material, and belongs to the technical field of biomedical material preparation. According to the method, metal pure titanium powder and hydroxyapatite ceramic powder are uniformly mixed; after the materials are uniformly mixed, the mixture is mixed with ammonium hydrogen carbonate to obtain mixed powder; the mixed powder is mechanically pressed into blocky pressed green compacts; then, the blocky pressed green compacts are put into a discharging plasma sintering furnace to be sintered in a gradient heating mode; demolding is performed, and the titanium/hydroxyapatite composite material is obtained. The porous titanium/hydroxyapatite composite material obtained through preparation has the characteristics that the elasticity modulus is low; the pore parameters are controllable; the physical adaptability is good, and the like. The porous titanium/hydroxyapatite composite material also has good bioactivity and bone combining capability. Along with the bone induction effect of the hydroxyapatite in the human body, the bone tissues are gradually induced to grow into the pores to realize the biological fixation, so that the bone integration effect is greatly improved. The material is particularly applicable to biomedical engineering with human body hard tissue defects, such as artificial joints, artificial bones and dental implants.

The invention relates to a preparation method of a nano-zinc oxide-doped hydroxyapatite porous ceramic and belongs to the technical field of preparation of biomedical materials. The preparation methodtakes nano-zinc powder and hydroxyapatite powder as the raw materials and comprises weighing out ZnO and HA (hydroxyapatite) at a weight ratio of 1-7%:99%-93%, performing ball milling to obtain composite powder, uniformly mixing and pressing the composite powder and a pore forming agent of ammonium bicarbonate to obtain a blank, and then preparing the nano-zinc oxide-doped hydroxyapatite porous ceramic through a spark plasma sintering method. The prepared nano-zinc oxide-doped hydroxyapatite porous ceramic is pure in composition and achieves a controllable porosity of 40-70%, and added nano-zinc oxide can improve the osteogenic induction capacity of the nano-zinc oxide-doped hydroxyapatite porous ceramic and further improve the bone-forming capacity of parts around an implanted part; by controlling the porosity of the nano-zinc oxide-doped hydroxyapatite porous ceramic, the preparation method can prepare biological ceramics which meet different requirements and are applied to bone scaffold, filling and repair materials.

The invention discloses an antibacterial functional gradient porous HA-Ag bone filling scaffold with an active interlayer and belongs to the technical field of biomedical material preparation. The functional gradient porous HA-Ag bone filling scaffold material with the active interlayer is prepared by weighing and ball-milling hydroxyapatite HA and Ag powder according to an ingredient proportion, weighing and mixing mixed powder and an ammonium bicarbonate pore former according to a porosity proportion, preparing three kinds of powder, then laying the powder in a gradient manner, mechanically pressing into a block pressed blank, putting the pressed blank into a discharge plasma sintering furnace, performing sintering after vacuuming a system to 2-6Pa, heating at a rate of 50-100min/DEG C, keeping at 800-1000 DEG C for 5-10min, and cooling to room temperature in the furnace. The material can induce adhesion and proliferation of bone cells on the surface of the material actively, has good osteogenesis activity and antibacterial property, high porosity and mechanical stability at the same time, and can serve as a good artificial bone filling material.

The invention provides an EBM molded titanium implant and a preparation method and application thereof and belongs to the technical field of biomedical implant materials. The preparation method of theEBM molded titanium implant comprises the step of carrying out aftertreatment on a titanium implant molded in an EBM manner, wherein the aftertreatment comprises ultrasonic acid etching and anodic oxidation. According to the preparation method, during the preparation of the EBM molded titanium implant, the EBM molded titanium implant is subjected to surface treatment by adopting ultrasonic acid etching and anodic oxidation, so that residual powder can be effectively removed, and meanwhile, a micro-nano multistage structure beneficial to cell adhesion, propagation and differentiation is prepared on the surface of the titanium implant. The preparation method provided by the invention is simple and convenient, the consumption of chemical reagents can be effectively lowered; meanwhile, the prepared titanium implant has a rich micro-nano multistage structure, and thus, the osteogenesis activity of the implant can be effectively promoted; meanwhile, the bone integration capability of the implant can be effectively enhanced, the success rate of planting is increased, and thus, the EBM molded titanium implant and the preparation method thereof have broad application prospects.

The invention relates to a preparation method of hydroxyapatite biological ceramics with strontium cooperated with an ordered micrometer structure for osteogenesis. The preparation method comprises the following steps: (1) preparing strontium doped hydroxyapatite powder; (2) uniformly mixing the strontium doped hydroxyapatite powder and a binder, placing the mixture in a mold with a bottom lined with an organic plastic mesh screen, dry pressing and molding, sintering at a high temperature, and cooling, thus obtaining the strontium doped hydroxyapatite ceramics. Compared with the prior art, byadopting the dry pressing and molding technology, the organic plastic mesh screen with a mesh aperture dimension of 25 to 75 micrometers is used as a template for regulating and controlling an orderedstructure, the structure with the surface having ordered micrometer patterns is prepared on the basis of the strontium doped hydroxyapatite, so that the hydroxyapatite biological ceramics have betteradhesion, proliferation and differentiation of osteoblast; and the preparation method is novel, simple, easy, and capable of preparing the strontium doped surface ordered micrometer structure hydroxyapatite biological ceramics having a synergistic osteogenic differentiation effect in a low-cost and large-scale manner without special equipment.

To cover shortages of existing PMMA bone cement products, including very high hardness and poor biocompatibility, the invention provides a mineralized collagen incorporated PMMA bone adhesive and filling material. Mineralized collagen (MC) is prepared via an in vitro biomimetic mineralization process and has a chemical composition and structure of self-assembled nano-sized calcium phosphate and collagen molecules, thus possessing biomimetic mineralized structure and mechanical properties similar to natural human bone, good biocompatibility, osteogenic activity and biodegradation ability. An MC incorporated bone adhesive and filling material with high compressive strength and low elastic modulus, and improved biocompatibility compared to pure PMMA bone cements may be obtained. Such bone adhesive and filling materials reduce the risk of abrading the host bone tissue and avoiding damage of the implant caused by extrusion, and form osteointegration with the host bone, improving the stability of the bone adhesive and filling materials at the implantation site.

The invention relates to a mineralized collagen based skull repairing device and a manufacturing method thereof. The mineralized collagen based skull repairing device is made of a mineralized collagen/PCL (polycaprolactone) composite material; and the appearance of the repairing device comprises a spherical segment cap at the upper part and a cylindrical plug at the lower part. A separation mold and a secondary pouring technology are adopted during preparation of the mineralized collagen based skull repairing device, and the product defect caused by air residue at the blind end of the mold is overcome. The skull repairing device made of the mineralized collagen/ PCL composite material has excellent osteogenic activity, the material is gradually and passively degraded under the creeping substitution action of cells, and growth of new bones can be guided at the skull defect part. A degradation product of the composite material is neutral, acidic materials causing aseptic inflammation cannot be generated, and new bone formation isn't affected; and through composition of PCL, the skull repairing device has excellent elasticity and toughness besides enough crush resistance and bending strength, and the repairing requirement of a skull of a human body is met.

The invention discloses a calcium phosphate-based composite microsphere support and a preparation method thereof. The preparation method comprises the following steps of dissolving polyglycollide-lactide in dichloromethane to obtain a polyglycollide-lactide solution; dispersing crystallized calcium phosphate and anhydrous calcium hydrogen phosphate in the polyglycollide-lactide solution to obtain a solution 1; adding Tween-80 to liquid wax to obtain a solution 2: adding the solution 1 to the solution 2 under a stirring state to obtain composite microspheres; pouring the composite microspheres into a mold, adding deionized water, conserving in a constant temperature and humidity chamber after demolding; immersing in cold acetone and freeze drying to obtain the calcium phosphate-based composite microsphere support. The prepared microsphere support has a three-dimensional through pore structure with moderate pore size, and has good mechanical properties and good biocompatibility. The pore size of the support is further enlarged after polyglycollide-lactide is degraded, thereby facilitating the formation of new bones. Besides, the preparation process is simple; raw materials are easily available; cost is low and industrialized production can be easily realized.

The invention relates to a composite artificial bone for enhancing osteogenic activity and a preparation method thereof. A human bone implant material needs to supply a certain compression resistance support at the implanted part to maintain and supply a spatial structure for cells to grow and shin, and in addition, a minienvironment similar to the environment of a natural bone needs to be provided so as to facilitate adhesion, proliferation and differentiation of cells, induce ingrowth of vessels, and finally to mediate generation of new bones. The preparation method of the composite artificial bone comprises the following steps: preparing a collagen aqueous solution, adding nano-hydroxyapatite, fully and uniformly mixing, pouring into a mold, prefreezing at 4 DEG C, -20 DEG C and -80 DEG C respectively, performing vacuum freeze drying, placing in a polyphenol solution for incubation, prefreezing at 4 DEG C, -20 DEG C and -80 DEG C respectively once again, and performing vacuum freeze drying. According to the composite artificial bone and the preparation method thereof disclosed by the invention, oleuropein is used for crosslinking, so the mechanical properties of stent materials are improved by virtue of bonding effects, and the osteogenic activity of the artificial bone stent materials are greatly improved by virtue of good antioxidant activity and osteogenic promotion property of the oleuropein.

The invention discloses a surface modified polyarylether bone implant material containing a phthalazinone biphenyl structure and a preparation method thereof. The polyarylether bone implant material comprises polyarylether containing the phthalazinone biphenyl structure, a polydopamine layer and an osteolite-like layer, wherein the surface of the polyarrylether is of a three dimensional structurewith holes; the polydopamine layer is attached to the surface of the polyarrylether; and the osteolite-like layer is attached to the surface of the polydopamine layer. The preparation method of the surface modified polyarylether bone implant material containing the phthalazinone biphenyl structure comprises the following steps: preparing the three dimensional structure with holes on the surface ofpolyarrylether; modifying polyarrylether by polydopamine; and preparing the osteolite-like layer. The polyarylether material has relatively high biocompatibility, osteogenic activity and surface coating stability.

The invention relates to a micro-arc oxidation method in a non-aqueous electrolyte system. The micro-arc oxidation method comprises the following steps that (1) an electrolyte is prepared, wherein a fluorine-containing compound is added into mixed alcohol, stirring is carried out until complete dissolving is achieved, cooling is carried out to the room temperature, then an additive is dissolved ina mixture, and the electrolyte is obtained; (2) sample pretreatment is carried out, wherein samples are ground, polished, rinsed, ultrasonically cleaned and blown to be dried for standby application;and (3) micro-arc oxidation is carried out, wherein the pre-treated samples connected with a micro-arc oxidation power supply anode and a stainless steel sheet connected with a cathode are placed inthe electrolyte, and then rinsing and blow drying are carried out after micro-arc oxidation treatment. The method is simple and easy to implement, and a prepared fluoride film layer can be applied tothe fields of acid environment corrosion resistance, biomedical magnesium alloy surface treatment and the like.

The invention provides a pearl powder polyamino acid composite bone repair material with osteogenic activity, and a preparation method and application thereof. The composite bone repair material is made of a pearl powder and an amino acid through in situ polymerization, wherein the amino acid is composed of a gamma-amino acid and a non-gamma-amino acid, and the non-gamma-amino acid is an alpha-amino acid and/or an epsilon-amino acid. The composite bone repair material of the invention has excellent mechanical properties and adjustable degradation performance, is convenient for meeting different needs, does not cause damage to tissues after degradation, has high osteogenic activity, has good biocompatibility and biological activity, and can be used as an excellent, non-toxic and novel biomedical material. When the amino acid is a specific amino acid of the invention, the mechanical strength of the obtained composite bone repair material is significantly improved, the strength of human natural cortical bone is met, and the composite bone repair material can be used as a bone repair material with excellent performance. In addition, the preparation method is simple, has no complicatedprocess, is easy to operate, can realize industrialized production, and has excellent application prospects.

The invention discloses a medical implant for improving cell osteogenesis performance and a preparation method thereof. The polished and cleaned medical metal is subjected to laser processing so as toobtain a surface structure with super-hydrophilic performance, and a statin drug coating is prepared on the super-hydrophilic surface, so that the drug loading is more uniform and sufficient. The medical implant with the drug coating has the function of enhancing the osteogenesis performance of human cells, is beneficial to rapid and tight combination with human tissues after being implanted intoa human body, is beneficial to smooth implementation of a transplantation operation, and effectively avoids secondary injury caused by transplantation failure.

The invention relates to a preparation method of a gradient porous bone scaffold material. The preparation method comprises the following steps: (1) preparing nano bioactive glass powder into a suspension solution A; (2) dissolving collagen and auxiliary materials into glacial acetic acid to prepare a suspension solution B; (3) adding the suspension solution A into the suspension solution C to obtain a suspension solution C; obtaining a suspension solution (1), a suspension solution (2) and a suspension solution (3), which have different water contents, respectively; (4) injecting the suspension solution (1) into a mold and freezing; after curing, injecting the suspension solution (2) and freezing; continually injecting the suspension solution (3) and freezing; then carrying out freeze drying; (5) carrying out crosslinking treatment; and (6) freezing and carrying out freeze drying, and finally carrying out sterilization treatment. The invention provides the gradient porous bone scaffold material prepared by the preparation method. By adopting the preparation method of the gradient porous bone scaffold material, the problem that a high-pore structure and a high mechanical property of the scaffold material cannot coexist is solved and the long-period stability of the bone scaffold material which is planted into a bone defect part is effectively improved; and meanwhile, the bone scaffold material is used as a carrier to realize space-time controlled release of loading cells or growth factors or medicines.

The invention discloses a preparation method of nano zinc loaded hydroxyapatite porous bioceramic and belongs to the technical field of biomecial material preparation. The method includes: weighing nano hydroxyapatite (99-93%) and nano zinc powder (1-7%) according to a mass ratio, ball-milling to obtain compound powder, mixing the compound powder with a pore former ammonium hydrogen carbonate, pressing after well mixing to obtain a blank, and utilizing discharging plasma sintering technology to prepare the nano zinc loaded hydroxyapatite porous bioceramic. The porous bioceramic prepared by themethod is uniform and pure in composition, porosity is controllable between 40-70%, compressive strength is 82-162Mpa, and pore size is 50-600um; by adding zinc, osteogenic inducing ability of the porous bioceramic is improved remarkably, and degradation rate is matched with osteogenesis rate; the porous bioceramic is suitable for bone stents and filling and repairing materials.

The invention discloses a method for preparing a multifunctional sodium alginate stent embedded with drug-loaded microspheres by using a 3D printing technology based on in-situ emulsification, and aims to provide a preparation method of a multifunctional bone defect repair stent material. The method is characterized by comprising the following steps: by taking a bioactive substance lecithin as an emulsifier, dispersing an amination modified polylactic acid solution dissolved with an antibacterial or anti-inflammatory drug into a sodium alginate solution to form a stable emulsion, then constructing the sodium alginate stent embedded with the drug-loaded microspheres in situ by utilizing a low-temperature 3D printing technology, and using divalent strontium ions (Sr<2+>) as a cross-linking agent to improve the mechanical properties and osteogenic activity of the stent. The method has the characteristics that the prepared tent can be individually designed according to the characteristics of the bone defect part of a patient, and the prepared stent has multiple functions of good biological activity, osteogenic ability, mechanical property, antisepsis, anti-inflammation and the like, and has potential application prospects in the field of bone tissue engineering.