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Gradient composite material and preparation method and application thereof

A composite material, gradient technology, applied in the fields of medical science, prosthesis, tissue regeneration, etc., to achieve the effect of controllable process, good hydrophilicity, and small surface roughness

Active Publication Date: 2018-08-17
上海积力威尔生物科技合伙企业(有限合伙)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a kind of gradient composite material with high-efficiency promotion of osteogenesis and fibrogenesis and can significantly improve the strength of tendon-bone bonding and its preparation method and application, so as to solve the problems existing in existing tendon-bone healing materials. one or more technical issues

Method used

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  • Gradient composite material and preparation method and application thereof
  • Gradient composite material and preparation method and application thereof
  • Gradient composite material and preparation method and application thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0032] In the first aspect, the present invention provides a method for preparing a gradient composite material. The method includes the following steps:

[0033] (1) Using organic solvents to formulate biocompatible polymers and bioactive ceramic powder into a variety of slurries with different contents of bioactive ceramic powder; and

[0034] (2) The multiple slurries prepared in step (1) are cast layer by layer or co-pressed by layered casting in a manner of increasing or decreasing bioactive ceramic powder content to obtain a gradient composite containing multilayer casting film material.

[0035] In the present invention, the multiple refers to two or more than two, the multi-layer refers to two and more than two layers; the layer-by-layer casting refers to placing a certain volume of a slurry Casting in the casting machine to obtain the first layer of cast film, and then on the basis of the first layer of cast film, another slurry is placed in the casting machine for casting ...

Embodiment 1

[0057] ①Mix the polycaprolactone and calcium silicophosphate powder in four 50mL sealed bottles according to different volume ratios of 1:0, 7:3, 5:5 and 3:7, and then put them into each sealed bottle Add a certain amount of dichloromethane to dissolve the polycaprolactone, and after the polycaprolactone is completely dissolved, 4 kinds of slurries with different volume percentages of uniformly dispersed calcium silicophosphate are obtained.

[0058] ②The 4 kinds of slurries obtained in ① were used for layered casting using the preparation parameters in Table 1 to prepare 4 kinds of single-layer casting films with increasing volume percentage of calcium silicophosphate. In Table 1, the casting volume indicates the amount of each slurry used for casting.

[0059] Perform performance tests on the four single-layer cast films prepared in this example:

[0060] figure 1 The backscattered surface topography (SEM image) of the four single-layer cast films prepared in this example. by fig...

Embodiment 2

[0062] The adhesion experiments of mouse osteoblasts and fibroblasts were carried out on the 4 kinds of cast monolayer films obtained in Example 1. After each sample of monolayer cast film was sterilized by 75% alcohol for 2 hours, the density was 5×10 4 Cells / mL 1mL of cell suspension was inoculated on the surface of the sample. After culturing for a specific time (1, 4, 24h), wash each monolayer cast film sample twice with phosphate buffered saline solution (PBS buffer), and use the mass fraction Fix the cells with 4% paraformaldehyde, wash with PBS buffer, add 500 μL volume of 0.1% Triton 100 for cell permeabilization, and finally use rhodamine dye and 4,6-diamidine- 2-Phenylindole (DAPI) stains the cytoskeleton and nucleus of the cells, and observes the cytoskeleton and nucleus under a fluorescence microscope.

[0063] figure 2 The experimental results of the morphology observation of osteoblasts on the surface of the four single-layer cast film samples of this example. by ...

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Abstract

The invention relates to a gradient composite material and a preparation method and an application thereof. The method comprises the steps: preparing a variety of slurries with different contents of bioactive ceramic powder from a biocompatible polymer and the bioactive ceramic powder with an organic solvent; and carrying out layer-by-layer tap casting or layering tap casting co-pressing of a variety of slurries in a manner of progressively increasing or decreasing the contents of the bioactive ceramic powder, to prepare the gradient composite material containing a plurality of layers of tap-casted films. The gradient composite material preferably contains four layers of tape-casted films, and the volume percentage contents of the bioactive ceramic powder are 0%, 30%, 50% and 70% from thefirst-layer tape-casted film to the fourth-layer tape-casted film respectively. The prepared gradient composite material can promote the early adhesion and proliferation of osteoblasts and fibroblastsand effectively promote the formation of transition layers between tendons and bone tissues, and the tendon-bone bonding strength can be increased by 38.6%. The gradient composite material provided by the invention can be used as a tendon-bone healing patch material, and is a quite potential biomedical material.

Description

Technical field [0001] The invention belongs to the technical field of biomedical material preparation, and particularly relates to a gradient composite material for tendon-bone healing, and a preparation method and application thereof. Background technique [0002] The tendon-bone anchor point is an important area connecting two different tissues, tendon-bone, and plays a key role as a bridge between tendon and bone. Investigations and studies have found that in people over 50 years old, the probability of partial or complete tearing at the tendon-bone stop of the rotator cuff is as high as 30-50%. In China, with the popularization of national fitness and more and more attention to health, the probability of tendon-bone injury caused by excessive or improper exercise is also increasing year by year. [0003] The tendon-bone attachment point is connected with a highly ordered layered part of a millimeter in two phases of tendon and bone. In the physiological and mechanical tests,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/10A61L27/12A61L27/18A61L27/50
CPCA61L27/10A61L27/12A61L27/18A61L27/50A61L2430/02A61L2430/10C08L67/04
Inventor 宁聪琴郭劲书赵金忠苏为
Owner 上海积力威尔生物科技合伙企业(有限合伙)
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