23 results about "Osteoblast" patented technology

The application discloses a functional composition for improving bone and joint health, a conditioner and application. The functional composition comprises a first active component and a second active component; the first active component comprises elastin peptide, haematococcus pluvialis and ergothioneine; and the second active component comprises one or more of houttuynia cordata and non-denatured type II collagen. The application realizes the protection effect on joint, cartilage and bone health by scientific matching of the components and synergistically improving the glycosaminoglycan level in chondrocytes and the osteocalcin level in osteoblasts. Meanwhile, the components in the functional composition for improving bone and joint health are natural and have good biocompatibility, and have no obvious side effects, so that the application provides a kind of drug or a substitute for the treatment and prevention of bone diseases.

The application discloses an active peptide for promoting bone growth, and a preparation method and application thereof. The active peptide contains amino acid sequences GPAGPLGPV, LGVPLPL, FLPEPPQEE and SFLPEPPQE, and is derived from an enzymatic product of animal bone collagen. The average molecular weight of the enzymatic product is 500-1000 Da, and the mass percentage of components with a molecular weight less than 1000 Da is greater than 60%. The preparation method comprises the following steps: sequentially performing acid treatment, alkali treatment, extraction, filtration and ion exchange on animal bone, and then performing enzymolysis on the animal bone under the action of a protease to obtain an enzymolysis solution, wherein the protease is selected from alkaline protease, trypsin and papain; and the enzymolysis solution is separated and purified, wherein the separation and purification comprises ultrafiltration membrane grading and chromatographic separation, the cut-off molecular weight of the ultrafiltration membrane is 3kDa, and the chromatographic separation comprises gel chromatography and reverse-phase high performance liquid chromatography separation. The low-molecular active peptide obtained by the application can promote the proliferation and / or differentiation of osteoblasts, improve the alkaline phosphatase activity, has a small molecular weight and is easy to be absorbed and utilized by the body, has a wide raw material source, has a simple and controllable preparation process, and has a good application prospect.

This invention belongs to the field of genetic engineering technology and discloses a high-efficiency expression method for recombinant collagenase and its application in the preparation of bovine bone collagen peptides. The collagenase gene derived from Bacillus sp. HUB-I-004 strain (accession number J9CTX3) was optimized using E. coli-preferred codons. A recombinant expression vector was constructed and transformed into E. coli BL21(DE3). After optimization of induction conditions and Ni-NTA affinity purification, a highly active recombinant collagenase with a specific activity of 231.05 U / mg was obtained. Bovine bone collagen was extracted using a high-temperature, high-pressure method. Bovine bone collagen peptides were prepared under optimal conditions of a substrate concentration of 10 mg / mL, an enzyme addition of 20%, and enzymatic hydrolysis at 37℃ for 8 h, significantly improving peptide yield. The obtained product is mainly composed of small molecule peptides and exhibits significant osteoblast proliferation-promoting activity, making it widely applicable in the preparation of health foods and pharmaceuticals.

The application relates to the technical field of natural high-molecular compound processing and medical materials, and discloses a method for preparing mineralized collagen based on a synchronous biomimetic calcification and siliconization strategy. The preparation method comprises the following steps: dissolving collagen in a buffer solution, adding a soluble calcium ion solution, adjusting the pH value to 8-10 under the condition of an ice water bath, then culturing under stirring and constant temperature conditions for 15-30 min; then adding a silicate and phosphate mixed solution dropwise under stirring, adjusting the pH value to 8-10, and then constant temperature culture for 12-24 h; and the mineralized collagen material prepared by separating the precipitate, washing with water and freeze-drying has synchronous biomimetic calcification and siliconization. The composite material constructed by the application has a high mineralization degree close to that of natural bone, exhibits good biocompatibility, can effectively promote the proliferation and differentiation of osteoblasts, and has a good application prospect in the field of bone repair and the like.

The application discloses an autogenous bone block grinding device for oral implantation and relates to the technical field of special instruments for oral implantation. The autogenous bone block grinding device comprises a grinding cylinder, the grinding cylinder comprises an outer cylinder and an inner cylinder, and a driving shaft rod is rotatably arranged in the inner cylinder. A driving assembly is arranged on the top sidewall of the driving shaft rod, the driving end of the driving assembly is arranged in the interlayer groove between the outer cylinder and the inner cylinder, the driving end of the driving assembly is fixedly provided with an anti-sloshing assembly, the anti-sloshing assembly can realize bidirectional clamping and limiting of the bone block in the grinding disc, the upper narrow and lower wide arc-shaped groove on the anti-sloshing limiting plate is matched, the irregular bone block shape is matched, the contact area is increased, the circumferential slip and the radial jumping of the bone block during grinding are effectively prevented, the dry friction between the bone block and the grinding structure is reduced, the local friction heating is reduced, the osteoblasts and active factors are prevented from being damaged, and the biological activity of the autogenous bone is maximally reserved.

This invention discloses a Hu sheep active peptide complex and its application in bone health care. Using healthy Hu sheep bones as raw material, collagen is extracted after defatting and decalcification. This collagen is then enzymatically hydrolyzed with trypsin and alkaline protease, followed by ultrafiltration, desalting, and freeze-drying to obtain Hu sheep bone collagen peptides. These collagen peptides are mixed with pharmaceutical excipients, calcium source compounds, chondroprotective agents, and fat-soluble vitamins. An ethanol-water binder solution containing palmitoyl aspartate diethyl ester and hesperidin-3-O-glucoside is added, and the mixture is granulated, dried, sized, and pressed to obtain the active peptide complex. This complex, through the synergistic effect of its components, can significantly promote osteoblast proliferation and inhibit apoptosis, effectively increasing bone density in osteoporosis model animals. It also exhibits excellent biocompatibility and no cytotoxicity, solving the problems of limited function, insufficient preparation efficiency, and instability of existing single active peptides. This makes it suitable for the development and application of bone health-related nutritional supplements.

The present application relates to the technical field of medicine, in particular to a MK-4 analogue, a preparation method and application thereof. The MK-4 analogue provided by the present application is more stable in liver microsomes and has a longer half-life. In the osteogenic experiment of osteoblasts, the osteogenic ability is stronger, which is of great significance for enhancing the specificity, effectiveness and reducing the side effects of drugs, and thus can be used for preparing drugs for preventing and / or treating osteoporosis. As the MK-4 analogue, it is expected to be used for preparing drugs for treating cardiovascular and cerebrovascular diseases, chronic kidney disease, neurodegenerative diseases, diabetes and cancer, etc.

A method for promoting bone healing in a subject includes administering to the subject in need thereof a composition including Lactobacillus plantarum TWK10 deposited at China General Microbiological Culture Collection Center with an accession number CGMCC No. 13008. The composition can effectively promote activation and generation of osteoblasts, and bone maturation.

The application provides a polytetrafluoroethylene barrier film and its application and preparation method, and belongs to the technical field of biomedical materials. The polytetrafluoroethylene barrier film comprises a film body and a bioactive layer on the surface of the film body; wherein the material of the film body comprises polytetrafluoroethylene, and the outer surface of the film body has a polar group; the material of the bioactive layer comprises a hydrophilic bioactive molecule. In the polytetrafluoroethylene barrier film provided by the application, the polar group on the outer surface of the polytetrafluoroethylene film body interacts with and effectively combines with the hydrophilic bioactive molecule, the combination stability between the film body and the bioactive layer is improved, the adhesion and growth of osteoblasts can be realized, and then the new bone mass during the bone augmentation surgery using the polytetrafluoroethylene barrier film is improved. In addition, the hydrophilic bioactive molecule can also endow the polytetrafluoroethylene barrier film with multifunctionality due to its characteristics.

Mesenchymal stem cells and osteoblast progenitor cells are induced to differentiate into osteoblasts. Provided is a culture medium for stem cell culture containing 60 vol% to 90 vol% of a basal culture medium and 10 vol% to 40 vol% of physiological saline, and further having, added thereto, 1 ng / mL to 100 ng / mL of EGF, 0.2 ng / mL to 20.0 ng / mL of FGF-2, 0.2 ng / mL to 20.0 ng / mL of PDGF, and 0.5 mM to 8.0 mM of magnesium ascorbyl phosphate.

This application relates to the field of biotechnology, and particularly to collagen peptides that promote osteoblast proliferation and differentiation and increase bone density, as well as their preparation methods and applications. The method includes (A) soaking coarsely shaped cartilage in an alkaline solution, followed by washing the soaked cartilage; (B) removing grease from the cartilage; (C) washing the grease-removed cartilage with an acid solution; (D) subjecting the acid-washed cartilage to high-pressure heat treatment using microwave heating; (E) stepwise enzymatic hydrolysis, including first hydrolyzing the cartilage with a protease, followed by further hydrolysis with chondroitin sulfate ABC enzyme; and (F) purifying the enzyme-inactivated hydrolysate by alcohol precipitation. The product obtained by this method exhibits good acid resistance and is rich in small molecule chondroitin sulfate.

The application discloses a bone-strengthening milk protein extract and a preparation method and application thereof, and belongs to the technical field of deep processing of cow milk. The bone-strengthening milk protein extract contains a bone-strengthening active peptide FYPELFR, and the preparation method specifically comprises the following steps: (1) pasteurization; (2) casein separation; (3) pepsin hydrolysis; (4) trypsin hydrolysis; (5) purification and desalination; and (6) concentration and drying. The bone-strengthening active peptide and the bone-strengthening milk protein extract containing the same can be combined with integrin alpha v beta 1 through hydrophobic interaction and hydrogen bonding, can promote the proliferation and differentiation of osteoblasts by activating specific signal pathways, can promote the proliferation of osteoblasts, can be used for the development of bone-strengthening functional food or medicine, and has important significance for the deep processing of cow milk.

This invention discloses a method for preparing peptide-calcium chelates and a method for confirming their osteogenic physiological activity. The prepared peptide-calcium chelates exhibit good thermal stability and osteoblast proliferation-promoting activity. The combined use of ultrasound and microwave treatment significantly enhances the chelation effect between peptides and calcium. The method for confirming the osteogenic physiological activity of peptide-calcium chelates includes steps such as preparing samples of the peptide-calcium chelates to be confirmed, rat animal experiments, sample preparation, comparison of bone turnover markers, comparison of bone microstructure, and histological comparison. This method has a good ability to evaluate the anti-osteoporosis activity of peptide-calcium chelates and can provide a theoretical basis for guiding the future development of bioactive functional foods using collagen peptide-calcium chelates.

The present application belongs to the technical field of egg yolk protein deep processing and functional active polypeptide preparation, and relates to the technical field of preparation and separation of calcium absorption promoting peptides, and aims to improve the preparation efficiency and preparation source of food-derived calcium absorption promoting peptides. The present application discloses a method for preparing calcium absorption promoting peptides from defatted egg yolk powder, and the preparation method and application thereof. The method directly uses defatted egg yolk powder as the starting material, and then neutral protease and trypsin are added in sequence for complex enzymolysis. After enzyme inactivation, separation and drying, the product is obtained. Through the step-by-step synergistic effect of double enzymes, the functional peptide segments with phosphoserine modification, VVGS(p)AEAGVDAASVSEEFRADHPF; LGGVGSPVSGGGKQLAS(p); IVLSVNITS(p); NPLS(p)YTSAK, can be efficiently released and enriched, so that the final product has excellent calcium ion chelating capacity and significant osteoblast promoting activity. The product can be directly used as a functional ingredient in various food and health care products related to bone health.

The application belongs to the technical field of biomedical materials, and particularly relates to an enzymatic polypeptide-based hydrogel as well as a preparation method and application thereof. The enzymatic polypeptide-based hydrogel comprises gelatin, bioactive polypeptides and TGase enzyme, and the gelatin and the bioactive polypeptides are connected through the TGase enzyme; the amino acid sequence of the bioactive polypeptide is Q x GRGDS, wherein x is an integer selected from 1-5. The hydrogel improves the mechanical strength and the bioactivity through a physical-chemical double network structure, eliminates the interference of metal ions, realizes the directional differentiation regulation of osteoblasts, and effectively promotes the proliferation and mineralization of the osteoblasts. The enzymatic polypeptide hydrogel has the characteristics of easy synthesis and control, good biocompatibility and low biological toxicity, and has great application prospect in bone tissue engineering.

This invention relates to the field of bone repair materials technology, and discloses a bone repair material utilizing deer bone peptides and its preparation method. The method includes the preparation of deer bone extract, the preparation of melon seed extract, the formulation of a composite peptide solution, the preparation of a carrier base solution, and the compounding and molding. By using a composite peptide solution containing deer bone peptides and melon seed peptides, the synergistic effect of the two peptides enables the material to possess excellent osteoblast proliferation, differentiation, and anti-inflammatory activity, thereby promoting the repair and regeneration of bone defects and enhancing the bioactivity and therapeutic targeting of the material. By mixing the composite peptide solution with a carrier base solution containing a biocompatible carrier material and adding a cross-linking agent for cross-linking, a stable structure is formed between the peptides and the carrier material, thereby ensuring that the material has good mechanical support properties and a controllable degradation rate, ensuring that the material maintains its morphology in the early stages of implantation and guides new bone growth in an orderly manner over time.

Provided is the use of a mulberry total polyphenol extract in the preparation of a product for promoting bone formation. The active ingredients of the mulberry total polyphenol extract include phenolic acids, polyphenol derivatives, flavones, isoflavones and tannins. The product for promoting bone formation can promote osteoblast proliferation, promote osteogenic calcification, inhibit sclerostin activity, and / or promote bone growth.

This invention relates to a polypeptide derived from lactoferrin and its uses. The amino acid sequence of the polypeptide is shown in any one of SEQ ID NO:1 to SEQ ID NO:3. The polypeptide of this invention is not only non-toxic and highly safe, but also exhibits good osteogenic activity, specifically promoting osteoblast proliferation, differentiation, and mineralization, and increasing the calcium ion content within osteoblasts. Therefore, the polypeptide of this invention has a positive effect on bone development due to its good osteogenic activity and can be used in the preparation of food (e.g., infant formula).

The present invention relates to a novel lappaconitine derivative and a pharmaceutically acceptable salt thereof, a preparation method therefor, and a medical use thereof using osteogenesis-promoting activity. The lappaconitine derivative induces the differentiation of stem cells into preosteoblasts, and increases bone density when administered to an osteoporosis animal model, and induces osteogenesis, and thus can be effectively used for preventing, alleviating, or treating bone-related diseases such as osteoporosis.

This invention discloses a calcium phosphate ceramic particle-methacrylated gelatin hydrogel scaffold for osteoblasts pretreated with Tideglusib to enhance osteogenic differentiation, relating to the fields of biomedical engineering and bone tissue regeneration. It comprises osteoblasts pretreated with Tideglusib and activated, biphasic calcium phosphate ceramic particles, and a methacrylated gelatin hydrogel encapsulating the osteoblasts and ceramic particles. The preparation method includes: obtaining rat osteoblasts; seeding the osteoblasts onto the biphasic calcium phosphate ceramic particles; and pretreating the seeded biphasic calcium phosphate ceramic particles with Tideglusib solution to activate the Wnt signaling pathway and promote osteogenic differentiation. This invention exhibits excellent bone defect repair capabilities, good degradability and biocompatibility, provides necessary mechanical support, and has strong clinical translational potential.

The application discloses a camel blood serum polypeptide and application thereof in preparation of a product for promoting proliferation and / or differentiation of osteoblasts, and the amino acid sequence of the camel blood serum polypeptide is SEPSGTAFGGR, DEFF or EFL. Based on a camel blood serum peptide segment identified by LC-MS / MS, the target polypeptide is obtained from camel blood serum related polypeptides through computer virtual screening, and the MC3T3E1 cell is used for standardization of the proliferation activity of the osteoblasts. It is found through experiment verification that the camel blood serum polypeptide has significant proliferation and differentiation activity of the osteoblasts, and can be used as an active substance for promoting osteogenesis and used for bone tissue repair.

The application discloses a polypeptide-containing composition and its use for bone growth of children. The application also discloses a growth-promoting polypeptide which is screened and separated from a horse foal leg bone and can effectively promote proliferation of osteoblasts. The growth-promoting polypeptide can effectively promote weight increase of a rat femur, can significantly improve bone strength and bone density, can increase ALP activity, promote maturation of osteoblasts and start calcification, can significantly improve calcium ion content in the bone, and has a good effect of promoting bone growth and improving bone strength.

This invention discloses an osteosarcoma biomarker, relating to the field of tumor prognostic prediction technology. The osteosarcoma biomarker includes the genes CGREF1, COL13A1, KIF25, and RPL35AP7. This invention also discloses a method for constructing an osteosarcoma prognostic prediction model, using the expression levels of the biomarkers CGREF1, COL13A1, KIF25, and RPL35AP7 and their corresponding multivariate analysis coefficients to construct the osteosarcoma prognostic prediction model. The osteosarcoma biomarker and its screening method provided by this invention, with osteoblasts as the core research object, avoid the problem of mutual masking of signals from different cells in overall transcriptome analysis. By mapping osteoblast-specific molecular characteristics to the patient's overall transcriptome data, it achieves clinical translational application.