103 results about "Osteoblast" patented technology

The invention discloses an antler bone strengthening peptide for promoting bone repair as well as a screening method and application thereof, and belongs to the technical field of traditional Chinese medicinal material polypeptides. And the antler bone strengthening peptide is one of G40, G44 and G45. Grinding and crushing the antler, homogenizing the lysate, performing ultrasonic centrifugation, precipitating protein with methanol, centrifuging and discarding the supernatant; extracting, desalting, and freeze-drying to obtain a antler polypeptide extract; liquid chromatography-mass spectrometry detection, Peaks 8 search software and De Novo method analysis are adopted, according to the conditions that ALC is larger than or equal to 95%, local context is larger than or equal to 95%, Area is larger than or equal to 1000000 and an NCBI database, free polypeptide is obtained through comparison, the biological activity probability, the peptide fragment toxicity and the sensitization of the peptide fragment are predicted, and candidate polypeptide is obtained; synthesizing candidate polypeptides by a solid-phase synthesis method; the influence of the candidate polypeptide on osteoblast proliferation is evaluated, and the antler bone strengthening peptide is screened out. The antler bone strengthening peptide promotes osteoblast proliferation, and reveals a potential bone repair mechanism of the antler bone strengthening peptide.

The invention belongs to the technical field of medical osteogenesis materials, and particularly relates to a titanium-based material surface PDPASr (at) CO coating with osteogenesis promoting and antibacterial functions and a preparation method and application thereof.The preparation method comprises the steps that S1, a titanium-based material is soaked in an alkali solution and placed in a vacuum drying oven to be subjected to alkali heat treatment; s2, allowing the titanium-based material to stand under a dark condition, soaking the titanium-based material in a polydopamine solution for 20-28h, and then taking out the titanium-based material; s3, preparing a mixed solution system containing PAH, SrCl2 and NaOH, standing the titanium-based material under a dark condition, soaking the titanium-based material in the mixed solution system for 20-30 hours, and then taking out the titanium-based material; s4, the titanium-based material is soaked in a ferroferric dodecacarbonyl solution, the titanium-based material is taken out after being shaken and reacted for 20-30 h in a shaker under the dark condition, and the titanium-based material with the PDPASr (at) CO coating on the surface is obtained.The PDPASr (at) CO coating on the surface of the titanium-based material has the excellent osteogenesis performance, the spreading, early differentiation and late mineralization effects of osteoblasts can be obviously promoted, and the titanium-based material has the good application prospect. And under the condition of near-infrared light irradiation, the material shows excellent antibacterial performance.

The invention provides a nutrition enhancer beneficial to bone health of middle-aged and elderly people, a preparation method of the nutrition enhancer and application of the nutrition enhancer in preparation of medial care food, and belongs to the field of nutritious food. The nutrient supplement comprises a plant fermentation product, osteopontin peptide, collagen peptide, N-acetylglucosamine and calcium, vitamins and mineral substances can be added, and the plant fermentation product is obtained by performing anaerobic fermentation on rhizoma drynariae and chestnuts through a complex microbial inoculant composed of lactobacillus reuteri and bifidobacterium longum subsp. Longum; the collagen peptide is prepared by performing enzymolysis on bovine bones through alkaline protease and flavourzyme; the hydrogel is prepared by adopting a sodium alginate-calcium chloride-carboxymethyl chitosan micro-capsule embedding process. An in-vitro experiment shows that the nutrition enhancer can remarkably promote osteoblast proliferation, differentiation and mineralization; animal experiments show that the bone mineral density of osteoporosis model mice can be effectively improved. The nutrition enhancer disclosed by the invention is suitable for preparing care food and bone health products, and has a remarkable effect of improving bone health of middle-aged and elderly people.

The invention relates to the technical field of medical treatment, and particularly discloses dihydroartemisinin-loaded nano-hydrogel, which is prepared from the following components: 5 to 20 percent of gelatin methacryloyl, 5 to 15 percent of nano-hydroxyapatite and 0.5 to 2 percent of dihydroartemisinin, according to the invention, through the constructed Ge MA-nHA-DHA functionalized hydrogel system, the local concentration and bioavailability of dihydroartemisinin are remarkably improved, and the repair and regeneration of bone tissues are promoted. Research results show that the system can effectively inhibit inflammatory response, promote proliferation and differentiation of osteoblasts, improve the structure and function of bone tissues, slow down further collapse of femoral heads and finally improve the living quality of patients. In addition, the biological material shows good biocompatibility and appropriate drug release characteristics when applied in vivo, and a novel effective means is provided for treatment of steroid-induced femoral head necrosis.

The application discloses a functional composition for improving bone and joint health, a conditioner and application. The functional composition comprises a first active component and a second active component; the first active component comprises elastin peptide, haematococcus pluvialis and ergothioneine; and the second active component comprises one or more of houttuynia cordata and non-denatured type II collagen. The application realizes the protection effect on joint, cartilage and bone health by scientific matching of the components and synergistically improving the glycosaminoglycan level in chondrocytes and the osteocalcin level in osteoblasts. Meanwhile, the components in the functional composition for improving bone and joint health are natural and have good biocompatibility, and have no obvious side effects, so that the application provides a kind of drug or a substitute for the treatment and prevention of bone diseases.

The application discloses a jaw bone defect negative pressure repair body with a flushing function and a preserved periosteum, which comprises a base body, the base body is a titanium alloy net structure formed by 3D printing, two ends of the base body are adapted to be attached to bone broken ends, and an outer surface of the base body is adapted to be contacted with jaw bone periosteum, the base body is internally provided with a negative pressure suction pipeline penetrating through the base body, an outer pipe opening of the negative pressure suction pipeline is connectable to a negative pressure suction device, a pipe wall of the negative pressure suction pipeline is distributed with through holes formed by the titanium alloy net structure, and a reserved flushing pipeline is arranged around an outer periphery of the negative pressure suction pipeline, and the reserved flushing pipeline is distributed with flushing holes around the outer periphery. The repair body has the following functions: promoting growth and differentiation of osteoblasts on the surface of the bone broken ends and the periosteum, and promoting new bone formation; and being capable of discharging tissue exudates and the like out of the body.

The invention discloses oyster pallium hexapeptide with an effect of promoting osteogenesis as well as a preparation method and application of the oyster pallium hexapeptide. The amino acid sequence of the oyster pallium hexapeptide is Phe-Phe-Glu-Pro-Lys-Phe. Fresh oyster mantle is used as a raw material, pepsin and trypsin are used for hydrolysis and centrifugation to obtain supernate, then ultrafiltration is carried out to obtain filtrate with the molecular weight being 1000 Da or below, finally freeze drying is carried out, the sequence of oyster hydrolytic peptide is obtained through mass spectrum identification, and by means of a molecular docking technology and a database prediction simulation method, the oyster hydrolytic peptide is obtained. Oyster peptide with high osteogenesis promoting capacity is screened from a plurality of peptide sequences. The oyster hexapeptide obtained by the invention has the effects of improving osteoblast proliferation and differentiation capacity and improving dexamethasone-induced zebra fish bone loss, and can be used as a beneficial dietary supplement in related products for delaying bone loss.

The purpose of the present invention is to three-dimensionally culture osteoblasts produced with a direct conversion technique, and to provide a three-dimensional bone-like tissue composed of the osteoblasts and bone matrix. The present invention relates to, for example, a method for producing an osteoblast-containing three-dimensional cell mass, comprising: a first step for performing direct conversion from somatic cells of mammals into osteoblasts; a second step for subjecting cells having undergone the first step to high-density culture, and forming a cell sheet containing the osteoblasts; and a third step for obtaining an osteoblast-containing three-dimensional cell mass from the cell sheet obtained in the second step.

The invention relates to the technical field of metal surface treatment, in particular to a treatment process of a hydrophilic implant. According to the process, the implant is sequentially subjected to sand blasting and acid etching, compound acid solution activation, gradient alkaline neutralization, multi-stage ultrasonic cleaning, low-temperature plasma modification, nitrogen protection ultraviolet radiation and normal saline sealing storage treatment. Through a multi-step synergistic effect, a stable hydrophilic micro-nano structure is constructed on the surface of the implant, a large number of Ti-OH hydrophilic groups are formed, carbon and hydrogen atom adsorption is effectively isolated, and the problems that the hydrophilicity of an existing implant is prone to loss, osteoblast climbing is difficult, and the healing period is long are solved. The implant treated by the process is durable and stable in hydrophilicity, the healing period after implantation is shortened to about one month, the planting survival rate is remarkably increased, the process is green and safe, and industrial production can be realized.

This invention discloses a method for preparing leek seed extract and its applications. The method includes two-phase solvent extraction, crystallization, liquid-phase preparation of active ingredient A, gel column preparation of active ingredient B, and low-temperature drying. The preparation process of this invention is simple and efficient, and the obtained leek seed extract is light in color and has a strong effect on promoting osteoblast proliferation and differentiation. It can be applied in the fields of pharmaceuticals, food, and cosmetics related to the prevention of osteoporosis.

The invention belongs to the technical field of biomedical materials and bone tissue engineering, and discloses preparation and application of a self-piezoelectric aluminum nitride film / cavity assembly structure for promoting large-section critical bone defect repair. The thin film is based on the piezoelectric property of AlN, the self-power-generation capacity in the physiological vibration environment is achieved by optimizing the cavity structure, and the surface current density of AlN is remarkably improved. The film provided by the invention can continuously provide stable bio-electricity signals under the conditions of no polarization treatment and no external physical stimulation, so that the adhesion, proliferation and differentiation of osteoblasts are promoted. In-vivo experiments show that the film / cavity assembly structure can effectively repair the critical bone defect of the rabbit femur without a stent, and is superior to an existing piezoelectric material. The limitation that a traditional piezoelectric bone repair material depends on external stimulation and a stent is broken through, and an innovative strategy with high clinical transformation potential is provided for bone tissue repair.

The invention discloses a functional composition capable of improving bone and joint health, a conditioner and application. The functional composition comprises a first active component and a second active component, the first active component is prepared from elastin peptide, haematococcus pluvialis and ergothioneine; the second active component is prepared from one or more of herba houttuyniae and non-denatured type II collagen. Through scientific compatibility of all the components, the levels of glycosaminoglycan in chondrocytes and osteocalcin in osteoblasts are synergistically improved, and the protection effect on joint, cartilage and skeleton health is achieved. Meanwhile, a plurality of components in the functional composition capable of improving the health of the bones and the joints are natural in source, good in biocompatibility and free of obvious side effects, and a medicine or an alternative scheme thereof is provided for treatment and prevention of the bone diseases.

The application provides a traditional Chinese medicine granule for improving osteoporosis and a preparation method and application thereof, and belongs to the technical field of medicines. Codonopsis pilosula, drynaria rhizome, epimedium and eucommia are subjected to deep eutectic solvent extraction, purification, and preparation of a traditional Chinese medicine extract, water extraction, dialysis and drying of pilose antler to prepare pilose antler protein peptide; water extraction and alcohol precipitation of cistanche to prepare cistanche polysaccharide; after modification of hydroxyapatite by citric acid, sodium dihydrogen phosphate and beta-cyclodextrin, the product is prepared by inclusion of calcium salt, alpha-cholecalciferol and vitamin D3, and then mixed with the traditional Chinese medicine extract, the pilose antler protein peptide and the cistanche polysaccharide to prepare the traditional Chinese medicine granule for improving osteoporosis. The prepared traditional Chinese medicine granule for improving osteoporosis can promote the synthesis of collagen and bone matrix protein, inhibit bone resorption, promote bone remodeling and enhance the activity of osteoblasts, and has a good improvement effect on osteoporosis, bone fracture and other symptoms, and has a wide application prospect.

The application belongs to the field of biomedical materials and bone tissue engineering technology, and discloses a preparation and application of a self-piezoelectric aluminum nitride film / cavity assembly structure for promoting repair of large segment critical bone defects. The film is based on the piezoelectric properties of AlN, and through optimization of the cavity structure, self-power generation capability under a physiological vibration environment is realized, and the surface current density of AlN is significantly improved. The film can continuously provide stable bioelectric signals without polarization treatment and external physical stimulation, thereby promoting adhesion, proliferation and differentiation of osteoblasts. In vivo experiments show that the film / cavity assembly structure can effectively repair rabbit femoral critical bone defects without a scaffold, and is superior to existing piezoelectric materials. The application breaks through the limitations of traditional piezoelectric bone repair materials which depend on external stimulation and scaffolds, and provides an innovative strategy with high clinical conversion potential for bone tissue repair.

The invention provides application of disulfiram in preparation of a medicine for treating calcified aortic valve diseases, and belongs to the field of biological medicine. The disulfiram is used for intervening in-vitro cultured valvular interstitial cells and a mouse in-vivo calcified aortic valve stenosis model, and experimental results show that the disulfiram can inhibit the capability of differentiating the valvular interstitial cells to osteogenic phenotypes, relieve extracellular calcification deposition and relieve aortic valve calcification and stenosis. Therefore, the disulfiram can be used as a medicine for treating the calcified aortic valve disease and has a wide application prospect.

The application discloses an active peptide for promoting bone growth, and a preparation method and application thereof. The active peptide contains amino acid sequences GPAGPLGPV, LGVPLPL, FLPEPPQEE and SFLPEPPQE, and is derived from an enzymatic product of animal bone collagen. The average molecular weight of the enzymatic product is 500-1000 Da, and the mass percentage of components with a molecular weight less than 1000 Da is greater than 60%. The preparation method comprises the following steps: sequentially performing acid treatment, alkali treatment, extraction, filtration and ion exchange on animal bone, and then performing enzymolysis on the animal bone under the action of a protease to obtain an enzymolysis solution, wherein the protease is selected from alkaline protease, trypsin and papain; and the enzymolysis solution is separated and purified, wherein the separation and purification comprises ultrafiltration membrane grading and chromatographic separation, the cut-off molecular weight of the ultrafiltration membrane is 3kDa, and the chromatographic separation comprises gel chromatography and reverse-phase high performance liquid chromatography separation. The low-molecular active peptide obtained by the application can promote the proliferation and / or differentiation of osteoblasts, improve the alkaline phosphatase activity, has a small molecular weight and is easy to be absorbed and utilized by the body, has a wide raw material source, has a simple and controllable preparation process, and has a good application prospect.

To provide osteoblasts and compositions comprising the osteoblasts for treating bone diseases.SOLUTION: Disclosed is an osteoblast having both proliferation ability and differentiation ability, where the osteoblast has higher expression levels of connexin 43, RUNX2 and collagen type 1A compared with those of undifferentiated stem cells and mature osteocytes; higher expression levels of angiopoietin and alkaline phosphatase compared with those of undifferentiated stem cells; and lower expression levels of osterix, osteocalcin and osteopontin compared with those of mature osteocytes. Also, the osteoblast has a lower Ki-67 expression level compared with that of undifferentiated stem cells.SELECTED DRAWING: Figure 1

This invention relates to the field of biomedical materials technology, and more particularly to a method for preparing a hydroxyapatite bone tissue engineering scaffold, comprising the following steps: preparing acetylated or alkylated modified cellulose nanocrystals; using the modified cellulose nanocrystals as a biodispersant to synthesize hydroxyapatite; and using the synthesized hydroxyapatite to prepare a bone tissue engineering scaffold. The preparation method of this invention is simple, requires minimal experimental conditions, and does not require expensive equipment; it also does not introduce organic surfactants, and the dispersant used is essentially harmless to the environment and organisms; the prepared HA exhibits good dispersibility; and the hydroxyapatite bone tissue engineering scaffold has good biocompatibility, which is beneficial for osteoblast proliferation and differentiation, and for the structural remodeling and construction of bone defect tissue.

The invention discloses application of black bean source oligopeptide in preparation of a product for preventing / treating osteoporosis, and belongs to the technical field of bioactive peptides. According to the black bean source oligopeptide helpful for improving osteoporosis, the black bean source oligopeptide comprises oligopeptide KGVG and / or oligopeptide KIGT; wherein the amino acid sequence of the oligopeptide KGVG is Lys-Gly-Val-Gly, and the amino acid sequence of the oligopeptide KIGT is Lys-Ile-Gly-Thr; the black bean source oligopeptide can directly up-regulate osteoblast activity, enhance expression of BMP-2 and Runx2, improve the level of bone formation marker osteocalcin, remarkably improve the bone density and bone microstructure of ovariectomized mice and enhance bone mechanical strength, is non-toxic to other tissues, can be used as an effective nutrition strategy for treating and relieving osteoporosis, and has broad application prospects. A scientific basis is provided for efficient utilization of the black bean source bioactive peptide.

This invention belongs to the field of genetic engineering technology and discloses a high-efficiency expression method for recombinant collagenase and its application in the preparation of bovine bone collagen peptides. The collagenase gene derived from Bacillus sp. HUB-I-004 strain (accession number J9CTX3) was optimized using E. coli-preferred codons. A recombinant expression vector was constructed and transformed into E. coli BL21(DE3). After optimization of induction conditions and Ni-NTA affinity purification, a highly active recombinant collagenase with a specific activity of 231.05 U / mg was obtained. Bovine bone collagen was extracted using a high-temperature, high-pressure method. Bovine bone collagen peptides were prepared under optimal conditions of a substrate concentration of 10 mg / mL, an enzyme addition of 20%, and enzymatic hydrolysis at 37℃ for 8 h, significantly improving peptide yield. The obtained product is mainly composed of small molecule peptides and exhibits significant osteoblast proliferation-promoting activity, making it widely applicable in the preparation of health foods and pharmaceuticals.

The application discloses a kind of biocompatible materials and preparation method for bionic porous intervertebral fusion cage, belong to intervertebral fusion cage material technical field.A kind of biocompatible materials for bionic porous intervertebral fusion cage, by weight parts includes the following components: polyether ether ketone 65-80 parts, modified functional filler 15-25 parts, hydroxyapatite 8-12 parts, flow modifier 0.5-1 part;The modified functional filler is graphene oxide coated modified barium titanate functional filler.By introducing surface conductive modified nano piezoelectric ceramic particles, realize the effective conversion and transmission of mechanical stimulation to bioelectric signal, this microstructure design makes the insulating polyether ether ketone matrix possess the electric activity of simulating natural bone stress-electric signal response mechanism, so as to be favorable to activate osteoblast activity and promote bone integration of implant interface.

The invention discloses an interbody fusion cage based on a Thiessen polygonal gradient porous structure, and belongs to the technical field of biomedical engineering and orthopedic implants. The fusion cage body structure is of a three-dimensional communicated porous structure, the basic topological configuration of the porous structure is a Thiessen polygon, and the porous structure at least has gradient change in the distribution direction from the outer surface to the interior of the porous structure. The outer-layer small hole structure is beneficial for blood-source factors and osteoblasts to quickly enter and attach, so that osteogenesis is started; the inner-layer macroporous structure provides sufficient space for cell deep migration, blood vessel growth and new bone matrix deposition, sufficient osseointegration from outside to inside is finally achieved, the structure highly simulates a natural bone trabecula network, cell siphon attachment and internal connectivity are synergistically optimized through gradient pore design, and a specific post-treatment process is combined, so that the natural bone trabecula network structure is obtained. And the osseointegration performance and safety of the fusion cage are improved.

The invention provides application of xylotetraose or a composition of xylotetraose and nystose in preparation of an anti-osteoporosis product. The invention discloses the synergistic effect of the xylotetraose and the nystose in the aspect of osteoporosis resistance for the first time. When the zebrafish bone mineral composition and the zebrafish bone mineral composition are combined for use, the bone mineralization degree of zebrafish can be remarkably enhanced, the mineralization rate is increased, osteoblast activity is improved, bone metabolic disorder is effectively reversed, and the synergistic anti-osteoporosis potential of the zebrafish bone mineral composition is fully proved. Meanwhile, the invention also discloses the anti-osteoporosis effect of the xylotetraose for the first time, and the xylotetraose can promote bone mineralization, promote bone formation, inhibit bone resorption and regulate bone metabolism balance to a certain extent.

The application relates to the technical field of natural high-molecular compound processing and medical materials, and discloses a method for preparing mineralized collagen based on a synchronous biomimetic calcification and siliconization strategy. The preparation method comprises the following steps: dissolving collagen in a buffer solution, adding a soluble calcium ion solution, adjusting the pH value to 8-10 under the condition of an ice water bath, then culturing under stirring and constant temperature conditions for 15-30 min; then adding a silicate and phosphate mixed solution dropwise under stirring, adjusting the pH value to 8-10, and then constant temperature culture for 12-24 h; and the mineralized collagen material prepared by separating the precipitate, washing with water and freeze-drying has synchronous biomimetic calcification and siliconization. The composite material constructed by the application has a high mineralization degree close to that of natural bone, exhibits good biocompatibility, can effectively promote the proliferation and differentiation of osteoblasts, and has a good application prospect in the field of bone repair and the like.

The present application is based on the anatomical features of oral periodontal alveolar bone tissue and the regeneration speed difference of soft and hard tissues, and provides a method for preparing a gradient biomimetic scaffold including a soft tissue layer, a barrier layer and a hard tissue layer by using coaxial electrospinning technology. The gradient biomimetic scaffold exhibits good mechanical properties and biocompatibility. Among them, the gradient distribution of the soft tissue layer can induce the adhesion and regeneration of fibroblasts, the barrier layer at the interface can prevent fibroblasts from invading the osteogenic layer, and the hard tissue layer can induce the differentiation and regeneration of osteoblasts. The gradient biomimetic scaffold prepared by the present application can be used as an excellent guided oral soft and hard tissue combined repair material, and has a broad application prospect in oral medicine.

The present disclosure discloses an osteoporosis treatment system, apparatus, and method. According to the present disclosure, the osteoporosis treatment system includes: a solid gel pad that has a groove formed to be inclined in consideration of a main direction of trabeculae for an osteoporosis treatment area of a patient obtained using an imaging device; and a fixing part that fixes the solid gel pad to skins near treatment areas of each patient, wherein a probe of low-intensity pulsed ultrasound is inserted into the groove of the solid gel pad so that pulsed ultrasound applied by the low-intensity pulsed ultrasound amplifies movement of fluid inside a trabecular bone to maximize a response of osteoblasts distributed on a surface of the trabeculae.

The invention discloses a sodium niobate / molybdenum diselenide heterojunction and a preparation method and application thereof. The invention provides a sodium niobate / molybdenum diselenide heterojunction which comprises manganese-doped sodium niobate and molybdenum diselenide, and the ion molar ratio of Nb to Mo in the manganese-doped sodium niobate and molybdenum diselenide is 1.5: (0.5-3). The doped sodium niobate / molybdenum diselenide heterojunction has high piezoelectric performance and sonodynamic performance and has enzyme-like activity, under high-power ultrasonic driving, the sonodynamic and enzyme-like activity of the material is enhanced through the piezoelectric effect of the material, multiple ROS are generated, bacteria are killed in a synergistic mode, and biological membranes are removed; under the driving of low-power ultrasonic waves, oxidative stress materials are relieved, osteoblasts are proliferated and differentiated, and potential application prospects are achieved in the aspect of treating infectious bone tissues.

The application discloses an autogenous bone block grinding device for oral implantation and relates to the technical field of special instruments for oral implantation. The autogenous bone block grinding device comprises a grinding cylinder, the grinding cylinder comprises an outer cylinder and an inner cylinder, and a driving shaft rod is rotatably arranged in the inner cylinder. A driving assembly is arranged on the top sidewall of the driving shaft rod, the driving end of the driving assembly is arranged in the interlayer groove between the outer cylinder and the inner cylinder, the driving end of the driving assembly is fixedly provided with an anti-sloshing assembly, the anti-sloshing assembly can realize bidirectional clamping and limiting of the bone block in the grinding disc, the upper narrow and lower wide arc-shaped groove on the anti-sloshing limiting plate is matched, the irregular bone block shape is matched, the contact area is increased, the circumferential slip and the radial jumping of the bone block during grinding are effectively prevented, the dry friction between the bone block and the grinding structure is reduced, the local friction heating is reduced, the osteoblasts and active factors are prevented from being damaged, and the biological activity of the autogenous bone is maximally reserved.

The invention relates to the technical field of health-care wine, and discloses raspberry and fructus alpiniae oxyphyllae health-care wine and a preparation method thereof. The health-care wine takes 8-12 parts of raspberry and 1-3 parts of fructus alpiniae oxyphyllae which are homologous in medicine and food as core raw materials, preferably 10 parts of raspberry and 1.5 parts of fructus alpiniae oxyphyllae; the content of kaempferol-3-O-rutinoside in the raspberry is greater than or equal to 0.03%, and the content of volatile oil in the fructus alpiniae oxyphyllae is greater than or equal to 1.0% (ml The preparation method comprises the following steps: infiltrating 42.5-52% vol of solid-state white spirit base for 48 hours, continuously / intermittently and dynamically extracting by 18 times of volume, blending, aging by five-ten thousandth egg white powder for 30 days, and filtering with the turbidity of less than or equal to 1.0 NTU. Through the synergistic effect of raspberry and fructus alpiniae oxyphyllae, osteoblast activity is activated in a targeted manner, bone calcium deposition is promoted, and bone metabolism is improved in an auxiliary manner; the raw materials are safe and non-toxic, the process is scientific and controllable, the finished product is clear and transparent, medicine fragrance and wine fragrance are coordinated, the taste is mellow and mild, and a safe, effective and easily acceptable bone health care scheme is provided for people with osteoporosis.

The invention relates to the technical field of biological medicines and functional nucleic acid molecules, and discloses pearl mussel source miRNA and application thereof, a biological preparation and application thereof. The pearl mussel source miRNA is miRNA derived from a pearl mussel pallium exosome, contains a sequence shown in SEQ ID NO: 1, and can be modified by phosphorylation, locked nucleic acid and the like to enhance the stability. The biological preparation containing the miRNA can be used for preparing a medicine for preventing and / or treating osteoporosis or functional food for promoting bone mineralization, and the bone mineral density and the bone microstructure are improved by promoting osteoblast proliferation, differentiation and mineralization. According to the invention, marine biological resources which are natural in source and high in safety are utilized, and new active ingredients and technical schemes are provided for prevention and treatment of osteoporosis.