190 results about "Osteoblast" patented technology

The invention belongs to the technical field of biological medicine, and discloses a probiotic agent capable of improving the physiological activity function of vitamin D and maintaining bone health and application of the probiotic agent. The invention provides a probiotic agent composed of bifidobacterium adolescentis and bifidobacterium longum, the probiotic agent can significantly improve the bone mineral density, the bone trabecula thickness, the bone trabecula number, the bone trabecula connectivity and the bone volume fraction of an osteoporosis mouse, and meanwhile, the bone trabecula separation degree of the osteoporosis-removed mouse is significantly reduced; the level of vitamin D metabolites such as 25-hydroxy vitamin D and 1, 25-dihydroxy vitamin D in mouse serum is improved; the content of serum calcium and alkaline phosphatase in serum of mice is reduced, and the level of osteocalcin and type I procollagen amino terminal peptide in the serum is improved. The probiotic agent can relieve bone calcium loss, maintain bone health, repair bone injury, increase osteoblasts and reduce the osteoporosis degree, and can be used for preparing drugs for preventing and / or treating osteoporosis or preparing health care products or food beneficial to improving bone mineral density.

The invention relates to the technical field of biological medicine, in particular to recombinant III-type humanized collagen as well as a preparation method and application thereof. In one embodiment, the recombinant III type humanized collagen prepared by the invention has an amino acid sequence as shown in SEQ ID NO: 1 or SEQ ID NO: 4. The recombinant III-type humanized collagen prepared by the invention has good biological activity, can promote collagen synthesis related gene expression, promote skin fibroblast proliferation, increase the content of skin collagenous fibers and elastic fibers, and improve skin elasticity and compactness; in addition, the expression of osteogenesis related genes is promoted, and the proliferation of osteoblasts is promoted. The results show that the recombinant III-type humanized collagen prepared by the invention has an extremely wide application prospect in developing related medical and cosmetic products for delaying skin aging and promoting wound repair and bone tissue healing.

The invention discloses a cranio-maxillofacial dissimilar metal bone implant with an antibacterial property and a preparation method thereof, and relates to the technical field of medical implant prostheses, the preparation method comprises the following steps: step 1, preparing a nano-silver-antibacterial peptide GL13K composite peptide solution with the antibacterial property; 2, based on the selective laser melting technology, titanium alloy powder is prepared into a porous framework; 3, melting zinc powder, and filling pores of the porous skeleton with the molten zinc powder to obtain a semi-finished heterogeneous metal bone implant; step 4, depositing the nano-silver-antibacterial peptide GL13K composite peptide solution prepared in the step 1 on the surface of the semi-finished heterogeneous metal bone implant prepared in the step 3 through a pulse electrodeposition method to obtain a finished heterogeneous metal bone implant; the prepared finished heterogeneous metal bone implant has a remarkable antibacterial effect, the postoperative infection risk can be reduced, and the nano-silver-antibacterial peptide GL13K composite peptide can further promote osteoblast attachment and proliferation and accelerate the bone healing process.

The invention discloses an antler bone strengthening peptide for promoting bone repair as well as a screening method and application thereof, and belongs to the technical field of traditional Chinese medicinal material polypeptides. And the antler bone strengthening peptide is one of G40, G44 and G45. Grinding and crushing the antler, homogenizing the lysate, performing ultrasonic centrifugation, precipitating protein with methanol, centrifuging and discarding the supernatant; extracting, desalting, and freeze-drying to obtain a antler polypeptide extract; liquid chromatography-mass spectrometry detection, Peaks 8 search software and De Novo method analysis are adopted, according to the conditions that ALC is larger than or equal to 95%, local context is larger than or equal to 95%, Area is larger than or equal to 1000000 and an NCBI database, free polypeptide is obtained through comparison, the biological activity probability, the peptide fragment toxicity and the sensitization of the peptide fragment are predicted, and candidate polypeptide is obtained; synthesizing candidate polypeptides by a solid-phase synthesis method; the influence of the candidate polypeptide on osteoblast proliferation is evaluated, and the antler bone strengthening peptide is screened out. The antler bone strengthening peptide promotes osteoblast proliferation, and reveals a potential bone repair mechanism of the antler bone strengthening peptide.

The invention discloses a bone-strengthening lactoprotein extract as well as a preparation method and application thereof, and belongs to the technical field of deep processing of cow milk. The bone-strengthening milk protein extract contains bone-strengthening active peptide FYPELFR. The preparation method specifically comprises the following steps: (1) pasteurization; (2) separating casein; (3) pepsin hydrolysis; (4) trypsin hydrolysis; (5) purifying and desalting; and (6) concentrating and drying. The bone-strengthening active peptide and the bone-strengthening lactoprotein extract containing the bone-strengthening active peptide can be combined with integrin alpha v beta 1 through hydrophobic interaction and hydrogen bonds, proliferation and differentiation of osteoblasts are promoted by activating a specific signal channel, proliferation of the osteoblasts is promoted, and the bone-strengthening active peptide and the bone-strengthening lactoprotein extract can be used for development of bone-strengthening functional food or drugs and have important significance on deep processing of cow milk.

The invention discloses an eggshell membrane nonapeptide and application thereof in prevention and treatment of osteoporosis, and belongs to the technical field of biology. The eggshell membrane nonapeptide is characterized in that the amino acid sequence of the eggshell membrane nonapeptide is shown as SEQ ID NO.1, and the amino acid sequence of the eggshell membrane nonapeptide is specifically Val-Pro-Pro-Gly-Asp-Tyr-Leu-Phe-Gln. The eggshell membrane nonapeptide provided by the invention can repair and improve the oxidation resistance, differentiation activity and bone formation ability of osteoblasts influenced by oxidative stress, and provides a new treatment means for preventing and treating osteoporosis caused by oxidative stress.

The invention belongs to the technical field of biological medicines, and particularly relates to efficient mineralized hydrogel for bone repair as well as a preparation method and application of the efficient mineralized hydrogel. According to the hydrogel for promoting hard bone repair provided by the invention, hydroxybutyl chitosan (HBC) and deferoxamine (DFO) are introduced into a fibrinogen-thrombin / bioglass system, so that the in-vitro mineralization ability of the material is remarkably improved. HBC provides a good adhesion scaffold for osteoblasts and promotes formation of a mineralized matrix; dFO is chelated with iron ions, a hypoxia environment is simulated, expression of osteogenesis related genes is activated, and deposition of mineralized matrixes is further promoted. And more and denser inorganic mineral substances are deposited on the surface of the material through the synergistic effect of the two.

The invention belongs to the technical field of cell small molecule compound reprogramming and bone tissue engineering material culture, and discloses a method for inducing fibroblasts to be reprogrammed into osteoblasts. According to the invention, small molecule compounds act on fibroblasts, each small molecule is endowed with characteristics of a set of characteristic gene expression mode, and the small molecules are divided into different modules according to the expression characteristic mode, so that the fibroblasts of the cells are converted into osteogenic differentiation, the osteogenic differentiation effect is enhanced and maintained, and the in-vivo development of the cells is simulated to a great extent; therefore, healthy osteoblasts can be prepared from the skin fibroblasts, cell stress pressure can be resisted, and the prepared cells are free of tumorigenicity, safe and healthy. The osteoblast disclosed by the invention can be applied to preparation of bone materials and medicines for treating bone defects, for example, the osteoblast and a traditional bone material are used for constructing an active bone tissue engineering material, so that the use of allogeneic bones commonly used in clinic at present is avoided, and the occurrence of adverse ethical events is reduced.

The invention discloses a bone cement composite material with collapse resistance and a preparation method thereof, and belongs to the technical field of biomedical materials. The composite material comprises a solid phase part and a liquid phase part, the liquid phase part and the solid phase part are mixed according to the liquid-solid ratio of 0.35 mL / g to 0.6 mL / g; the solid phase part is composed of calcium sulfate powder and calcium phosphate powder, and the mass ratio of the calcium sulfate powder to the calcium phosphate powder is 85: 15; the liquid phase part is selected from carboxymethyl chitosan or a combination of carboxymethyl chitosan and sodium alginate. The bone cement composite material prepared by the preparation method disclosed by the invention has good biocompatibility; due to a gel network formed by carboxymethyl chitosan and sodium alginate, the compressive strength of the bone cement is remarkably improved, and the collapse resistance and injectable performance are excellent; meanwhile, the bone cement composite material promotes the attachment and proliferation of osteoblasts, and can induce osteogenic differentiation in vitro.

The invention belongs to the technical field of medical supplies, and discloses a newborn head shape correcting pillow which comprises a pillow body, a positioning seat is arranged at the top end of the pillow body, an air conveying port is fixed to the outer portion of the positioning seat, a self-adaptive mechanism is installed at the bottom end of the positioning seat, a traction mechanism is arranged in the positioning seat, and a measuring mechanism is installed in the pillow body. Through cooperation of micro motors, a winding drum, an elastic band and other structures, the device can make a flexible silica gel head ring sleeve the forehead of a newborn, the multiple sets of micro motors are started to drive the winding drum to wind the elastic band, the traction direction is cooperatively controlled through the double motors, vector adjustment in a three-dimensional space is achieved, and the device accurately acts on a skull malformation area; the intermittent traction force can activate osteoblast activity of skull sutures, increase the local bone deposition rate and correct the head shape of the newborn, so that the aim of conveniently improving the correction efficiency of the device is achieved.

The invention discloses a decellularized amniotic membrane matrix composite scaffold, a preparation method thereof and application of the decellularized amniotic membrane matrix composite scaffold in orbital bone defect repair, and relates to the technical field of biomedical engineering and biomedical materials. In order to solve the limitation of an existing orbit implant material, methacrylic anhydride (MA) is grafted to HAM, methacrylate HAMMA hydrogel is synthesized, and then the decellularized amnion matrix composite scaffold (HAMMA / HAP / BMP-2) is prepared by combining a simulated body fluid biomimetic mineralization method with 3D printing and ultraviolet curing. The composite scaffold material prepared by the invention can regulate the release of BMP-2 and maintain the effective drug concentration of a bone defect area so as to regulate the immune microenvironment of an early bone defect repair area, collect osteoblasts and accelerate the repair of bone defects.

The invention belongs to the technical field of medical osteogenesis materials, and particularly relates to a titanium-based material surface PDPASr (at) CO coating with osteogenesis promoting and antibacterial functions and a preparation method and application thereof.The preparation method comprises the steps that S1, a titanium-based material is soaked in an alkali solution and placed in a vacuum drying oven to be subjected to alkali heat treatment; s2, allowing the titanium-based material to stand under a dark condition, soaking the titanium-based material in a polydopamine solution for 20-28h, and then taking out the titanium-based material; s3, preparing a mixed solution system containing PAH, SrCl2 and NaOH, standing the titanium-based material under a dark condition, soaking the titanium-based material in the mixed solution system for 20-30 hours, and then taking out the titanium-based material; s4, the titanium-based material is soaked in a ferroferric dodecacarbonyl solution, the titanium-based material is taken out after being shaken and reacted for 20-30 h in a shaker under the dark condition, and the titanium-based material with the PDPASr (at) CO coating on the surface is obtained.The PDPASr (at) CO coating on the surface of the titanium-based material has the excellent osteogenesis performance, the spreading, early differentiation and late mineralization effects of osteoblasts can be obviously promoted, and the titanium-based material has the good application prospect. And under the condition of near-infrared light irradiation, the material shows excellent antibacterial performance.

The invention relates to the technical field of biomedical materials, in particular to a preparation process of novel composite bone cement with biological activity, which comprises the following steps: calcining calcium phosphate in a calcining furnace at 800-1000 DEG C for 3 hours, cooling to 20 DEG C, grinding the calcium phosphate by using a grinding machine until the diameter is less than 100 mu m, and drying to obtain the novel composite bone cement with biological activity. Calcium phosphate micro powder is obtained; by fusing the calcium phosphate, the nano-hydroxyapatite, the bioglass and the collagen, the material has a mechanical support function of an inorganic phase and biological activity of an organic phase, the nano-hydroxyapatite is extremely similar to human bone minerals and can promote osteoblast attachment and growth, and the collagen constructs a bionic microenvironment and accelerates new bone formation, so that the material can be used for treating osteoporosis. The bone repair efficiency is obviously improved.

The invention provides a nutrition enhancer beneficial to bone health of middle-aged and elderly people, a preparation method of the nutrition enhancer and application of the nutrition enhancer in preparation of medial care food, and belongs to the field of nutritious food. The nutrient supplement comprises a plant fermentation product, osteopontin peptide, collagen peptide, N-acetylglucosamine and calcium, vitamins and mineral substances can be added, and the plant fermentation product is obtained by performing anaerobic fermentation on rhizoma drynariae and chestnuts through a complex microbial inoculant composed of lactobacillus reuteri and bifidobacterium longum subsp. Longum; the collagen peptide is prepared by performing enzymolysis on bovine bones through alkaline protease and flavourzyme; the hydrogel is prepared by adopting a sodium alginate-calcium chloride-carboxymethyl chitosan micro-capsule embedding process. An in-vitro experiment shows that the nutrition enhancer can remarkably promote osteoblast proliferation, differentiation and mineralization; animal experiments show that the bone mineral density of osteoporosis model mice can be effectively improved. The nutrition enhancer disclosed by the invention is suitable for preparing care food and bone health products, and has a remarkable effect of improving bone health of middle-aged and elderly people.

The invention discloses bifidobacterium longum subsp.longum capable of improving the physiological activity function of vitamin D and maintaining bone health and application of the bifidobacterium longum subsp.longum, and belongs to the technical field of biological medicine. The bifidobacterium longum subsp. Longum CCFM1448 provided by the invention can obviously improve the total bone mineral density, the bone mineral density of distal femur bone trabecula, the thickness of the bone trabecula, the number of the bone trabecula, the connectivity of the bone trabecula and the bone volume fraction of an osteoporosis mouse, and obviously reduce the separation degree of the bone trabecula of the osteoporosis mouse; the content of a vitamin D metabolite 1, 25-dihydroxy vitamin D in serum of a mouse with osteoporosis is remarkably increased, the content of serum calcium and alkaline phosphatase in the serum of the mouse with osteoporosis is reduced, and the level of osteocalcin and I-type procollagen amino-terminal original peptide in the serum is increased. Therefore, the bifidobacterium longum subsp. Longum CCFM1448 has the effects of relieving bone calcium loss, maintaining bone health, repairing bone injury, increasing osteoblasts and reducing osteoporosis degree.

The invention discloses a preparation method and application of a manganese-containing bioglass composite membrane material, and belongs to the technical field of biomedical materials. The method for preparing the manganese-containing bioglass composite membrane material comprises the following steps: slowly adding a solution 1 into a solution 2, and stirring; adding TEP and stirring, adding calcium nitrate tetrahydrate and manganese nitrate tetrahydrate, and stirring to obtain a Mn-MBG spray precursor solution; carrying out spray drying and calcination on the Mn-MBG spray precursor solution; and adding hexafluoroisopropanol into the mixture, stirring to obtain an electrostatic spinning precursor solution, and carrying out electrostatic spinning. The composite membrane has the advantages of good anti-oxidative stress and anti-inflammatory effects, effective removal of ROS (reactive oxygen species), adjustment of M1 / M2 polarization of macrophages and the like. In addition, the composite membrane also has excellent mechanical properties, continuous ion release ability and remarkable biological activity, promotes functions of osteoblasts and vascular endothelial cells and regulates immune response, and can be applied to the field of bone defect repair.

The present invention relates to the field of oral implants, and in particular to a dopamine-doped BP@Sr-modified zirconia implant and its application. The dopamine-doped BP@Sr coating is used to modify inert interventional medical devices. 2+ Bio-multifunctional coating with black phosphorus nanosheets (BP@Sr). The high adhesion of dopamine can improve the biological inertness of zirconium oxide itself. It also improves the hydrophilicity and roughness of the sample, thereby promoting the adhesion and proliferation of osteoblasts on its surface, and making zirconium oxide have a certain photothermal effect and excellent resistance to bacterial microbial infection. It has potential clinical applicability and research value in orthopedic and dental implant devices.

The invention relates to application of Sirt1 in preparation of products for diagnosis, prognosis or treatment of steroid-induced femoral head necrosis, and belongs to the technical field of biotechnology and medicine. It is found that Sirt1 participates in dexamethasone-induced osteoblast ferroptosis, differential expression of Sirt1 can significantly affect the degree of dexamethasone-induced osteoblast ferroptosis, and occurrence of the steroid-induced femoral head necrosis phenomenon is prompted. The invention provides application of Sirt1 as a marker molecule for diagnosing steroid-induced femoral head necrosis in preparation of a reagent for diagnosing or screening steroid-induced femoral head necrosis, and provides application of an expression promoter of Sirt1 in preparation of a medicine for treating steroid-induced femoral head necrosis.

The invention provides hypoxia regulation exosome hydrogel and a preparation method and application thereof in bone defect repair, and compared with the prior art, the hypoxia regulation exosome hydrogel provided by the invention has the advantages that the exosome cultured by 3D stem cells modified by superparamagnetic ferroferric oxide nanoparticles is loaded on the GelMA hydrogel modified by bone targeting polypeptide, and the preparation method is simple. The intelligent oxygen-producing gel is innovatively constructed by skillfully utilizing the relationship between hypoxia and ROS generation according to the challenge that the hypoxia microenvironment of a bone defect part is closely followed. According to the hydrogel disclosed by the invention, osteogenesis regeneration is promoted by regulating and controlling a Wnt / beta-catenin pathway in osteoblasts, so that hypoxia and oxidative stress microenvironments are safely, efficiently and intelligently improved at injured parts. And the adhesion to the defect part is improved, and the problem that the superparamagnetic ferroferric oxide nanoparticles are difficult to effectively retain at the defect part is solved.

The invention relates to the technical field of medical treatment, and particularly discloses dihydroartemisinin-loaded nano-hydrogel, which is prepared from the following components: 5 to 20 percent of gelatin methacryloyl, 5 to 15 percent of nano-hydroxyapatite and 0.5 to 2 percent of dihydroartemisinin, according to the invention, through the constructed Ge MA-nHA-DHA functionalized hydrogel system, the local concentration and bioavailability of dihydroartemisinin are remarkably improved, and the repair and regeneration of bone tissues are promoted. Research results show that the system can effectively inhibit inflammatory response, promote proliferation and differentiation of osteoblasts, improve the structure and function of bone tissues, slow down further collapse of femoral heads and finally improve the living quality of patients. In addition, the biological material shows good biocompatibility and appropriate drug release characteristics when applied in vivo, and a novel effective means is provided for treatment of steroid-induced femoral head necrosis.

The invention discloses a milk-derived polypeptide for promoting osteoblast proliferation and differentiation and application thereof, and belongs to the technical field of bioactive peptides. The invention provides a milk-derived bioactive peptide WLKPDPS. The milk-derived bioactive peptide WLKPDPS has extremely high biological safety and a function of promoting osteoblast proliferation and differentiation. The milk-derived bioactive polypeptide WLKPDPS disclosed by the invention has an obvious effect of promoting osteoblast proliferation, can obviously promote osteoblast mineralization, and is expected to be used for preparing medicines for promoting osteoblast proliferation and preventing osteoporosis. The milk-derived bioactive polypeptide WLKPDPS is convenient to synthesize, can be industrially produced, and has a good application prospect in the fields of foods, medicines, cosmetics and the like.

The application discloses a functional composition for improving bone and joint health, a conditioner and application. The functional composition comprises a first active component and a second active component; the first active component comprises elastin peptide, haematococcus pluvialis and ergothioneine; and the second active component comprises one or more of houttuynia cordata and non-denatured type II collagen. The application realizes the protection effect on joint, cartilage and bone health by scientific matching of the components and synergistically improving the glycosaminoglycan level in chondrocytes and the osteocalcin level in osteoblasts. Meanwhile, the components in the functional composition for improving bone and joint health are natural and have good biocompatibility, and have no obvious side effects, so that the application provides a kind of drug or a substitute for the treatment and prevention of bone diseases.

The application discloses a jaw bone defect negative pressure repair body with a flushing function and a preserved periosteum, which comprises a base body, the base body is a titanium alloy net structure formed by 3D printing, two ends of the base body are adapted to be attached to bone broken ends, and an outer surface of the base body is adapted to be contacted with jaw bone periosteum, the base body is internally provided with a negative pressure suction pipeline penetrating through the base body, an outer pipe opening of the negative pressure suction pipeline is connectable to a negative pressure suction device, a pipe wall of the negative pressure suction pipeline is distributed with through holes formed by the titanium alloy net structure, and a reserved flushing pipeline is arranged around an outer periphery of the negative pressure suction pipeline, and the reserved flushing pipeline is distributed with flushing holes around the outer periphery. The repair body has the following functions: promoting growth and differentiation of osteoblasts on the surface of the bone broken ends and the periosteum, and promoting new bone formation; and being capable of discharging tissue exudates and the like out of the body.

The present invention discloses a polypeptide for enhancing osteoblast activity. The polypeptide of the present invention has stable chemical properties, is easy to synthesize on a large scale at low cost, has high biocompatibility, is non-toxic, and can be widely used in the prevention and treatment of orthopedic diseases.

The invention discloses a method for enriching bone collagen osteogenic active peptides based on affinity adsorption, including: installing a hydroxyapatite pre-packed column in an AKTA pure protein purification system, and enriching the bone collagen peptide solution by affinity adsorption through a hydroxyapatite column-AKTA pure protein purification system, wherein, firstly, eluting with mobile phase a as an eluting solution, collecting samples when an absorption peak occurs, and obtaining a bone collagen osteogenic active peptide sample F1, wherein the mobile phase a is a potassium chloride solution. The present invention screens out polypeptides affinity-adsorbed with a hydroxyapatite column matrix, obtains bone collagen peptides with strong ability to bind hydroxyapatite, measures the calcium binding capacity of the bone collagen peptide components obtained by enrichment and separation, and its effect on osteoblast proliferation, and provides technical support for the enrichment and preparation of affinity-adsorbed bone collagen osteogenic active peptides, which is of great significance to improving product effectiveness.

The present invention relates to the use of a polysaccharide from pitaya branches, and relates to the field of pharmaceutical technology. The present invention provides a use of a polysaccharide from pitaya branches in the treatment of osteoporosis. The polysaccharide is an extract obtained from pitaya branches by water extraction. The polysaccharide from pitaya branches promotes the proliferation and differentiation of osteoblasts, improves bone biomechanics, and is significantly effective in preventing and treating osteoporosis.

The invention discloses oyster pallium hexapeptide with an effect of promoting osteogenesis as well as a preparation method and application of the oyster pallium hexapeptide. The amino acid sequence of the oyster pallium hexapeptide is Phe-Phe-Glu-Pro-Lys-Phe. Fresh oyster mantle is used as a raw material, pepsin and trypsin are used for hydrolysis and centrifugation to obtain supernate, then ultrafiltration is carried out to obtain filtrate with the molecular weight being 1000 Da or below, finally freeze drying is carried out, the sequence of oyster hydrolytic peptide is obtained through mass spectrum identification, and by means of a molecular docking technology and a database prediction simulation method, the oyster hydrolytic peptide is obtained. Oyster peptide with high osteogenesis promoting capacity is screened from a plurality of peptide sequences. The oyster hexapeptide obtained by the invention has the effects of improving osteoblast proliferation and differentiation capacity and improving dexamethasone-induced zebra fish bone loss, and can be used as a beneficial dietary supplement in related products for delaying bone loss.

The purpose of the present invention is to three-dimensionally culture osteoblasts produced with a direct conversion technique, and to provide a three-dimensional bone-like tissue composed of the osteoblasts and bone matrix. The present invention relates to, for example, a method for producing an osteoblast-containing three-dimensional cell mass, comprising: a first step for performing direct conversion from somatic cells of mammals into osteoblasts; a second step for subjecting cells having undergone the first step to high-density culture, and forming a cell sheet containing the osteoblasts; and a third step for obtaining an osteoblast-containing three-dimensional cell mass from the cell sheet obtained in the second step.

The invention relates to the technical field of metal surface treatment, in particular to a treatment process of a hydrophilic implant. According to the process, the implant is sequentially subjected to sand blasting and acid etching, compound acid solution activation, gradient alkaline neutralization, multi-stage ultrasonic cleaning, low-temperature plasma modification, nitrogen protection ultraviolet radiation and normal saline sealing storage treatment. Through a multi-step synergistic effect, a stable hydrophilic micro-nano structure is constructed on the surface of the implant, a large number of Ti-OH hydrophilic groups are formed, carbon and hydrogen atom adsorption is effectively isolated, and the problems that the hydrophilicity of an existing implant is prone to loss, osteoblast climbing is difficult, and the healing period is long are solved. The implant treated by the process is durable and stable in hydrophilicity, the healing period after implantation is shortened to about one month, the planting survival rate is remarkably increased, the process is green and safe, and industrial production can be realized.

The present invention relates to the biological field. Further, the present invention provides the use of iPSC induced differentiation into pancreatic progenitor cells combined with medication for the treatment of diabetes. Specifically, the present invention provides a monoclonal antibody that specifically inhibits the activity of BMP4, which can inhibit the differentiation of iPSC cells into common osteoblasts and adipocytes by inhibiting the activity of BMP4 protein, thereby promoting the transformation of iPSC into pancreatic progenitor cells and differentiation into islet-like cells. After the islet-like cells and ginsenoside Rb3 are combined to prepare a drug kit, the effect of lowering blood sugar can be significantly improved, and it has a good prospect for drug application.