Method and device for activating stem cells

a stem cell and activation method technology, applied in the field of stem cell activation devices and methods, can solve problems such as unintended side effects, and achieve the effects of enhancing an osteoblast phenotyp

Inactive Publication Date: 2010-05-13
DEPUY SYNTHES PROD INC
View PDF16 Cites 69 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0018]Another embodiment includes a device for activating bone marrow aspirate which includes a component for handling bone marrow aspirate drawn from a patient. The device also includes a component for exposing the bone marrow aspirate to an active agent in a manner effective for triggering mesenchymal stem cells to enhance an osteoblast phenotype.
[0019]Another device for activating bone marrow aspirate is described, including a component for mixing a bone graft substitute and a bone marrow aspirate drawn from a patient to form a mixture. The device further includes a component for exposing the mixture to a fixed or tethered active agent in a manner effective for triggering mesenchymal stem cells to enhance an osteoblast phenotype. Following exposure, BMA is separated from tethered or fixed active agent and can be mixed with a bone graft sub

Problems solved by technology

Moreover, the use of osteogenic medium involves addition of components to the cells (e.g., growth factors) t

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method and device for activating stem cells
  • Method and device for activating stem cells
  • Method and device for activating stem cells

Examples

Experimental program
Comparison scheme
Effect test

example 1

Materials and Methods

[0098]The following materials and methods were used to develop certain aspects of the invention.

Differentiation of Cells

[0099]Human mesenchymal stem cells (Lonza, Walkersville, Md.) at passage 3 were seeded in basal medium (Stem Cell Technologies, Vancouver, Canada) at a density of 6×104 cells / 35 mm well and incubated for 2 days at 37° C. Cells were rinsed with PBS, activated with 100 ng / ml of growth factor (FGF-2 or TGFβ3, R&D Systems, Minneapolis, Minn.) in fresh basal medium for 1 hour, rinsed with PBS and then incubated in either fresh basal medium or osteogenic differentiation medium (Stem Cell Technologies, Vancouver, Canada) for 7-14 days at 37° C.

Real time PCR

[0100]Total RNA was prepared from cells treated with various active agents using RNeasy Plus Mini Kit and QIA shredder Mini Spin columns (Qiagen). Total RNA was also prepared from untreated cells as a control. cDNA was generated using random hexamer and Oligo dT following the TaqMan Reverse Transcri...

example 2

Results

[0106]Primary human mesenchymal stem cells are capable of differentiating down an osteoblastic lineage. This is demonstrated in vitro by culturing cells in an osteogenic cocktail containing, but not limited to, dexamethasone, ascorbic acid and β-glycerophosphate. Under these culture conditions cells show an upregulation of osteoblast differentiation markers, the most common of which is the early marker alkaline phosphatase.

[0107]FIG. 1 shows a slight upregulation in alkaline phosphatase activity in the untreated mesenchymal stem cells (circles) from days 10 to 12, as expected. However, when the mesenchymal stem cells were pretreated with TGFβ3 (triangles) or FGF-2 (squares) for 1 hour, followed by removal of the agents and culture in differentiation media, the level of alkaline phosphatase activity increased significantly 2-3 fold.

[0108]These data indicate that just a 1 hour treatment of mesenchymal stem cells with either TGFβ3 or FGF-2 on day 0 can impact osteoblast differen...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Timeaaaaaaaaaa
Timeaaaaaaaaaa
Timeaaaaaaaaaa
Login to view more

Abstract

Invention embodiments described herein include methods and devices for stimulating mesenchymal stem cells in a stem cell source to differentiate into osteoblasts capable of forming bone. Devices and methods described include exposing a stem cell source, such as bone marrow aspirate, adipose tissue and/or purified allogenic stem cells, to an active agent, in a manner effective to form activated stem cells.

Description

RELATED APPLICATIONS[0001]This patent application claims the benefit of the filing date of U.S. Provisional Patent Application Ser. No. 61 / 110,096, filed Oct. 31, 2008, and entitled, “DEVICE FOR ACTIVATING BONE MARROW ASPIRATE USING EX VIVO STIMULATION BY GROWTH FACTORS FOR IMPROVED BONE FORMATION”, and of U.S. Provisional Patent Application Ser. No. 61 / 152,335, filed Feb. 13, 2009, and entitled, “METHOD AND DEVICE FOR FORMING A BONE MARROW ASPIRATE PRODUCT”, the contents of which are incorporated herein by reference in their entirety.FIELD[0002]Inventive subject matter described herein relates to devices and methods for activating stem cells, including activating stem cells in bone marrow aspirate using ex vivo stimulation. The inventive subject matter also relates to implants containing such activated stem cells.BACKGROUND OF THE INVENTION[0003]In order to provide for maximum bone formation, it is desirable to transplant cells that already exhibit an osteoblastic phenotype, becaus...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K35/12A61P19/00C12M1/00C12M1/24
CPCA61L27/12A61L27/18A61L27/20A61L27/3834A61L27/3847C12N5/0663A61L27/46A61L27/3895C08L67/04C08L1/02A61P1/02A61P19/00A61P19/02A61F2/28A61L27/38A61L27/3821A61L27/44A61K35/32C12N5/0654C12N2501/115C12N2501/135C12N2501/15C12N2501/155C12N2506/1346
Inventor HANS, MEREDITHBUECHTER, DOUGGRUSKIN, ELLIOTTHORNSBY, STEPHENBROWN, MELISSA
Owner DEPUY SYNTHES PROD INC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap