531 results about "Adipose tissue" patented technology

The invention discloses carboxymethyl chitosan-hyaluronic acid-stem cell exosome eye drops as well as a preparation method and application thereof, and relates to the technical field of eye drops and medical biogel. The carboxymethyl chitosan-hyaluronic acid-stem cell exosome eye drops are prepared from tannic acid modified carboxymethyl chitosan and adipose tissue-derived stromal cell exosome freeze-dried powder, the carboxymethyl chitosan-hyaluronic acid-stem cell exosome eye drops provided by the invention can effectively treat xerophthalmia, and are stable in components, safe and reliable. The three components including the tannic acid modified carboxymethyl chitosan, the hyaluronic acid and the adipose tissue-derived stromal cell exosome coexist, so that the functions of all the components can be fully played, a synergistic effect is achieved, and a better treatment effect on xerophthalmia is achieved.

The present invention relates to the technical field of microorganisms. Disclosed are lacticaseibacillus paracasei and a postbiotic product prepared therefrom, and a use of both. The present invention provides lacticaseibacillus paracasei (Latin name) YJS05199, which has the preservation number of GDMCC No.63668, and was preserved in Guangdong Microbial Culture Collection Center on 19 July 2023. The present invention further provides a postbiotic prepared by applying the lacticaseibacillus paracasei. The lacticaseibacillus paracasei and the postbiotic prepared therefrom have a bacteriostatic effect, can inhibit the weight gain of a mouse fed with high-fat diets, reduce the adipose tissue weight and the blood glucose concentration, and can remarkably reduce the levels of TG, TC, and LDL-C in serum of the mouse. Thus, the lacticaseibacillus paracasei and the postbiotic prepared therefrom have great application prospects in preparation of products for preventing or treating obesity and related diseases or in preparation of bacteriostatic products.

The invention provides a preparation method of cereal fermentation broth postbiotics and application of the cereal fermentation broth postbiotics in improvement of systemic chronic inflammation and reduction of cholesterol and low-density lipoprotein, and belongs to the technical field of fermentation engineering. The invention provides a preparation method of cereal fermentation broth metabiotics, which is characterized in that mixed cereals are used as raw materials, anaerobic fermentation is carried out by using compound enzymes and various bacteria, and polysaccharides and proteins in the cereal raw materials can be quickly converted into functional small molecular substances, such as polypeptides, total phenols and total flavonoids. The cereal fermentation broth metagen can inhibit secretion of a macrophage proinflammatory medium, inhibit generation of inflammatory factors of liver, heart and intestinal tissues, and improve activation of adipose tissue macrophages related to body inflammation. After the cereal fermentation liquor is subjected to post-biogen treatment, glucose and lipid metabolism disorder can be effectively improved, liver function damage can be relieved, and the intestinal barrier function can be effectively improved.

The invention provides a stem cell exosome composition for treating erectile dysfunction and application thereof, the stem cell exosome composition comprises stem cell exosome and vasoactive intestinal peptide, and the amino acid sequence of the vasoactive intestinal peptide is shown as SEQ ID NO: 2. The amino acid structure of the vasoactive intestinal peptide is modified, so that the biological stability can be improved; the vasoactive intestinal peptide is combined with the adipose-derived mesenchymal stem cell exosome for use, so that the ED symptom can be relieved; the vasoactive intestinal peptide and the adipose tissue-derived stem cell exosome are combined for use, so that the expression level of cell factors can be adjusted, and the microenvironment of penis tissues can be improved.

The invention discloses a method for preparing an animal adipose tissue simulant by using soybean protein and application of the animal adipose tissue simulant, and belongs to the technical field of deep processing of vegetable protein. Soybean protein is used as a raw material, the soybean protein and polysaccharide are compounded and heated to form thermal induction gel, then microgel particles are obtained through mechanical shearing and crushing, and recombinant soybean protein emulsion gel, namely the animal adipose tissue simulant, is prepared through vegetable oil emulsification and glutamine transaminase covalent cross-linking. The fat-reducing sausage is used as a substitute of pig back fat and is applied to development of fat-reducing sausages. The animal adipose tissue simulant prepared by the invention has texture and melting characteristics similar to those of animal pig back fat. The invention provides the preparation method of the plant-based fat simulant, which meets the requirements of environmental protection and health and sustainable production, not only meets the requirements of texture and melting characteristics of solid fat, but also provides technical support for high-value application of soybean protein in the field of plant meat foods.

The invention relates to the field of biomedical materials, and particularly discloses a combined biovalve material anti-calcification method and a biovalve material. A combined bioprosthetic valve material anti-calcification method comprises the following steps: taking an animal-derived biological material, cleaning, removing adipose tissue, cutting a precordial region part to obtain a pericardium material, and storing the pericardium material in ice normal saline for later use; mixing the pericardium material and the decellularized treatment fluid A, oscillating and cleaning; adding the pericardium material into the decellularized treatment solution B, oscillating and cleaning; carrying out crosslinking treatment by utilizing a glutaraldehyde pericardium material; adding the crosslinked pericardium material into a reducing agent solution, oscillating and cleaning; the method comprises the following steps: putting a pericardium material into a glycerol-ethanol mixed solution or a glycerol-isopropanol mixed solution, and soaking for more than 24 hours; according to the anti-calcification method, on the basis that the mechanical property of the valve material is effectively maintained, the anti-calcification performance is improved, and the durability of the valve material is improved.

The invention relates to a regenerated decellularized adipose derivative as well as a preparation method and application thereof. The preparation raw material of the regenerated decellularized adipose derivative is prepared from a vascular matrix component (SVF) separated from human adipose tissue through decellularization treatment and freeze drying; the prepared regenerated decellularized adipose derivative is a porous material, the porosity is greater than 90%, and the aperture is 50-200 [mu] m; the composition comprises the following components in percentage by weight: 60-70% of collagen, 15-20% of elastin, 1 * 10 < 10 >-5 * 10 < 10 > particles / g of exosome, less than or equal to 5 ng / mg of DNA (deoxyribonucleic acid) residue, more than or equal to 30 ng / g of VEGF (vascular endothelial growth factor) and more than or equal to 15 ng / g of FGF-2 (fibroblast growth factor). The prepared regenerated decellularized adipose derivative is low in DNA residual quantity, rich in growth factors and capable of meeting the requirement for deep tissue filling due to the fact that the collagen content and the elastin content are high.

The invention relates to the technical field of microorganisms, in particular to enterococcus faecalis EF-1 capable of reducing blood lipid, a microbial preparation and application of the enterococcus faecalis EF-1. The enterococcus faecalis EF-1 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on September 27, 2024, and the preservation number is CGMCC No.32097. The enterococcus faecalis EF-1 is named as enterococcus faecalis EF-1. The enterococcus faecalis EF-1 has the functions of: (1) relieving the weight increase speed of animals, reducing body weight gain, and preventing, relieving or treating obesity; (2) the contents of triglyceride, cholesterol and low-density lipoprotein cholesterol in liver and serum are reduced; (3) the liver function is protected, and the contents of ALT and AST in serum and liver are reduced; (4) liver lipid deposition and lesion are relieved; (5) relieving adipose tissue hypertrophy; (6) preventing, relieving or treating metabolic syndrome; (7) reducing blood sugar, improving insulin resistance and relieving diabetes mellitus; 8, increasing the content of short-chain fatty acid; and (9) regulating intestinal flora.

The invention discloses a gastrodia elata polypeptide extraction process, and belongs to the technical field of polypeptide extraction. According to the method, gastrodia elata cell membranes are broken through acetone pretreatment, so that protein and lipid are dissociated, trimethylsilyl ether passivates active groups, immobilized enzyme decomposes cellulose and adipose tissues through normal-temperature enzymolysis, a protein stabilizer prevents oxidation and inhibits enzyme activity, differential pressure extraction is combined with a sodium pyrophosphate buffer solution, polypeptide is promoted to be dissolved out, and activity is maintained; the high extraction rate of the gastrodia elata polypeptide and low-active group destructiveness are realized. Reaction sites and binding force are increased by epoxy groups on the surface of an immobilized enzyme carrier prepared by modifying corn starch, enzyme molecules are stable and appropriate due to good biocompatibility, enzyme molecule conformation is optimized through covalent binding, a rigid structure is enhanced, enzyme activity is kept at a high level for a long time, continuous and efficient enzymolysis is performed in gastrodia elata polypeptide extraction, and the gastrodia elata polypeptide extraction efficiency is improved. Compared with the traditional free enzyme, the gastrodia elata polypeptide enzyme has the advantages that the enzyme use cost is reduced, the enzyme consumption and waste generation are reduced, and an efficient and economical scheme is provided for gastrodia elata polypeptide extraction.

The invention provides a method for constructing a CT-MRI (Computed Tomography-Magnetic Resonance Imaging) personalized three-dimensional heart model based on multi-modal imaging, which comprises the following steps of: firstly, carrying out myocardial and infarction region segmentation on a CMR-LGE image, and carrying out myocardial and intramyocardial adipose tissue segmentation on a CE-CT image to generate a corresponding Label image; then, a high-precision three-dimensional volume mesh model of the corresponding ventricle is constructed based on the segmentation results of the two types of images; and then, mapping the corresponding Label image with the tissue type to a corresponding finite element model according to the coordinates of the grid, and obtaining a corresponding personalized ventricular model with the tissue type. An interpolation method based on a general ventricular coordinate system is adopted, data of an MR ventricular model with infarct tissue attributes is transferred to a CT ventricular model, and therefore a mixed CT-MRI ventricular model is constructed. And finally, storing the constructed CT-MRI ventricular model data with the integrated tissue attributes as a standard geometric and topological file format.

The invention belongs to the technical field of biological medicine, and particularly relates to a stem cell preparation based on immunoregulation and a preparation method and application thereof. The stem cell preparation based on immunoregulation is prepared from the following raw materials in parts by weight: 5 to 40 parts of freeze-dried powder, 40 to 120 parts of gastrodia elata polysaccharide and 2 to 6 parts of melatonin, the freeze-dried powder is obtained by mixing and freeze-drying a stem cell culture solution and curcumin lipidosome; the volume ratio of the stem cell culture solution to the curcumin liposome is 1: (10-32); the protein concentration in the stem cell culture solution is 1.2 mg / mL to 1.6 mg / mL. The stem cell preparation provided by the invention can utilize the immune regulation effect of the mesenchymal stem cells to realize the immune repair of adipose tissues.

The invention relates to the field of microorganism sampling detection, in particular to escherichia coli sampling detection equipment for mouse experiments. The technical problem to be solved is that when mouse intestinal escherichia coli is sampled and detected, attention needs to be highly concentrated during manual operation, physical and mental exhaustion is easily caused, and the intestinal tract is damaged by slight carelessness. In addition, in the prior art, adipose tissue existing on the outer surface of the intestinal tract cannot be removed, and therefore the sampling detection result is affected. According to the technical scheme, the escherichia coli sampling and detecting equipment for the mouse experiment comprises a sampling box, a fixing assembly and the like; and a fixing assembly is mounted in the sampling box. According to the device, the left end and the right end of the intestinal tract of a mouse are fixed through the first clamping block and the second clamping block respectively, sterile water is injected into the intestinal tract of the mouse through an injection pipe for flushing, and cleaning of the intestinal tract of the mouse is completed, so that the pressure of manual operation is relieved, and meanwhile accidental damage to the intestinal tract of the mouse caused by manual operation is reduced.

The invention relates to brevibacillus parabrevis and application thereof in tissue isolation, and belongs to the technical field of biology. The brevibacillus parabrevis JN-6 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation number is CGMCC NO.29951, and the preservation date is March 7, 2024. The brevibacillus parabrevis JN-6 disclosed by the invention has the advantages that the preservation number is CGMCC NO.29951; the brevibacillus parabrevis provided by the invention has collagen degradation capability, and the produced collagenase can be used for adipose tissue separation and is further cultured into adipose-derived stem cells. When the produced collagenase is used for digestion and separation, the number of obtained cells can be 6.17 * 10 < 6 > / mL, the adipose-derived stem cells can be effectively differentiated, and the method has a good application prospect.

The invention discloses a raspberry homogeneous polysaccharide. The monosaccharide components of the raspberry homogeneous polysaccharide comprise fucose, rhamnose, arabinose, galactose, xylose, galacturonic acid, glucuronic acid and apiose. The main chain structure of the raspberry homogeneous polysaccharide is 1, 4-linked galacturonic acid pyranose, and the weight-average molecular weight of the raspberry homogeneous polysaccharide is 8.54 + / -5.0 kDa. The invention discloses a raspberry homogeneous polysaccharide component for inhibiting dietary oil absorption and preventing and improving obesity, which is prepared by the steps of water extraction and alcohol precipitation, ion exchange chromatographic column elution and permeation gel chromatographic column elution. The structure of the compound is analyzed by high performance gel permeation chromatography, infrared spectroscopy, ion chromatography, gas chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy. The homogeneous polysaccharide component can be used for improving obesity and fat tissue accumulation hypertrophy, and the efficacy of the homogeneous polysaccharide component is related to inhibition of absorption of dietary oil in the intestinal tract and promotion of discharge of the dietary oil into the body.

The utility model relates to the technical field of biomedical treatment, in particular to an adipose-derived mesenchymal stem cell sampling device which comprises a shell, a centrifugal motor is fixedly connected to the inner end of the bottom side of the shell, a rotating shaft is in output connection with the upper end of the centrifugal motor, and a plurality of centrifugal frames are fixedly connected to the outer end of the rotating shaft; the upper end of the centrifugal tube is sleeved with a test tube cover in a threaded mode, and the lower end of the test tube cover is fixedly connected with a shearing device. The adipose mesenchymal stem cell sampling device has the beneficial effects that by arranging the shearing device, adipose tissues can be directly sheared through the shearing device when being sheared, so that the shearing efficiency is improved, the sheared adipose tissues are ensured not to have the phenomena of insufficient and non-uniform shearing, and the sampling quality of the adipose mesenchymal stem cells is improved. And the high efficiency of subsequent centrifugal treatment is ensured.

The invention provides an application of lactobacillus rhamnosus NKU FL1-8 in resisting obesity, serum inflammation and cognitive impairment caused by high fat diet, and belongs to the technical field of microorganisms. The lactobacillus rhamnosus NKU FL1-8 has the advantages that the lactobacillus rhamnosus NKU FL1-8 can be used for resisting the obesity, the serum inflammation and the cognitive impairment caused by high fat diet; the invention finds that the lactobacillus rhamnosus NKU FL1-8 can obviously reduce adipose tissue increase caused by high-fat diet, relieve liver function impairment caused by high-fat diet, inhibit serum inflammation caused by high-fat diet, improve cognitive impairment caused by high-fat diet, strengthen intestinal barrier, increase the content of short-chain fatty acid in cecum and excrement and improve the immunity of the cecum. The intestinal flora is regulated, and the diversity of the intestinal flora is improved, so that related diseases caused by high-fat diet are effectively resisted.

Lymphedema is characterized by the accumulation of protein-rich interstitial fluid, lipids and a significant inflammatory cell infiltrate in the limb. It causes a significant morbidity and is a common disabling disease affecting more than 250 million people worldwide, however there is no curative treatment for lymphedema. Here, the inventors found that dermolipectomies from patient with lymphedema exhibit inflamed gene expression profile compared to normal arm on same patient. After lipidomic analysis, the inventors identified severe decrease in arachidonic acid-derived lipid mediators generated by the 15-lipoxygenase (15-LOX) in lymphedematous arms. Using a mouse model of lymphedema, they reproduced the etiology of the human pathology including the loss of specialized pro-resolving lipid mediators that play essential roles in resolution of inflammation. This was associated with a lack of regulatory T cells (Treg) recruitment in the injured limb adipose tissue. Importantly, the inventors identified the lymphatic endothelial 15-LOX was responsible for the chemoattraction and transendothelial migration of Tregs. These results were confirmed by an aggravation of lymphedema and deterioration of the lymphatic network in an original transgenic mouse model in which ALOX15 gene is selectively deleted in the lymphatic system. Importantly, this was reversed by the injection of 15-LOX expressing lentivectors. These results provide evidence that lymphatic lipoxygenase may represent a novel therapeutic target for lymphedema by serving as a mediator of Treg invasion into lymphedematous adipose tissue.

Described are systems and methods to determine one or more regional body composition values for one or more regions of a body based on a processing of one or more two-dimensional images that include a representation of the body. Regional body composition values may include any of a fat tissue mass, a lean muscle mass, a bone mineral density, a skeletal muscle mass, etc., which may be determined for any one or more regions of the body, such as the right arm, left arm, right leg, left leg, trunk, android, gynoid, etc.

The invention discloses a coronary artery and pericoronal adipose tissue segmentation system based on self-supervised learning, and belongs to the technical field of medical image analysis. The problem that an existing supervised learning model needs to depend on a large-scale annotation data set is solved. According to the method, the CCTA image is obtained through the coronary artery CT radiography technology, and the coronary artery segmentation model is pre-trained on the unannotated CCTA image in a self-supervised learning mode, so that the model learns rich feature representation; and then coronary artery segmentation model fine tuning is carried out on annotation data, so that the dependence on a large-scale annotation data set is reduced. The pericoronal fat segmentation algorithm adopts a traditional image processing algorithm, does not need annotation, and saves the network training cost. According to the method, an efficient segmentation framework is constructed, and the problem of high cost required in existing coronary artery and peripheral adipose tissue segmentation work is solved. The method can be applied to coronary artery and pericoronal adipose tissue segmentation.

The invention provides a fat acellular matrix hydrogel material and a preparation method thereof. The fat freeze-dried powder is subjected to decellularization, digestion, acid-base balance regulation and gelation to prepare the fat freeze-dried powder. According to the method disclosed by the invention, a chemical decellularization method that liquid nitrogen freeze crushing is combined with 0.5% SDS and 1% Triton X-100 compound decellularization liquid is adopted, and fat is further removed through isopropyl alcohol treatment; adipose tissues can be uniformly crushed into powder through liquid nitrogen freezing and crushing, the action area of decellularization liquid is increased, the thoroughness of decellularization is remarkably improved, and the problem of fat or decellularization liquid residue can be avoided through multiple times of pure water and alcohol replacement treatment; according to the method, the mild decellularization solution is adopted for treatment for 12 hours, so that damage to key components of the matrix due to high-concentration chemical reagents and long-time action is effectively avoided. According to the method disclosed by the invention, immunological rejection caused by cell residues and adverse effects of fat residues on biocompatibility and mechanical properties can be remarkably reduced, so that the clinical applicability of the hydrogel is improved.

The invention relates to an injectable acellular fat matrix composition as well as a preparation method and application thereof. Specifically, the invention provides an injectable decellularized fat matrix composition prepared by a high-pressure homogenization-enzymolysis coupling method, and the method comprises the following steps: performing high-pressure homogenization on a decellularized fat matrix to obtain matrix particles, performing protease enzymolysis, and performing freeze drying to obtain the injectable decellularized fat matrix composition. Therefore, the acellular fat matrix composition with excellent injectability, biological activity retention rate and long-term skin thickening effect is prepared.

The invention relates to a compound probiotic for preventing, relieving or treating hyperlipemia. The compound probiotic comprises a Bifidobacterium animalis subsp. Lactis C-2 strain with the preservation number of GDMCC (China General Microbiological Culture Collection Center) No: 65455 and a Lactobacillus fermentum GLF-217 strain with the preservation number of CGMCC No.28336. The invention further relates to a preparation method of the compound probiotic for preventing, relieving or treating the hyperlipemia. The two strains have potential interaction and can cooperate with each other to achieve synergistic interaction, and the effect of preventing, relieving or treating hyperlipemia is remarkably improved through compounding of the two strains. Specifically, the weight and the fat content are obviously reduced, the blood sugar and blood fat level is improved, the immunity is regulated, the antioxidant level is improved, the fat tissue metabolism level is regulated, and the gastrointestinal tract short-chain fatty acid metabolism is improved.

The invention discloses a Dunella strain, polysaccharide produced by the Dunella strain and application of the Dunella strain. The preservation number of the Duronella G3 is CCTCC (China Center For Type Culture Collection) NO: M 2024137, and the exopolysaccharide TDG3 produced by the Duronella G3 is used as a TLR4 / DC-SIGN dual receptor agonist and has the effect of remarkably reducing body weight. The exopolysaccharide TDG3 disclosed by the invention can obviously reduce the appetite of mice, reduce the food intake, reduce the body weight and reduce the content of adipose tissues, can also relieve or prevent liver fatty degeneration caused by obesity, is safe and non-toxic, is suitable for preparing the medicines for reducing fat and losing weight, and has a relatively good clinical application value and a wide application prospect.

The present invention relates to the technical field of microorganisms, in particular to a Lactobacillus acidophilus GOLDGUT-LA100 having functions of lipid lowering, blood glucose lowering and weight loss and application thereof. The Lactobacillus acidophilus GOLDGUT-LA100 provided by the present invention is preserved in China General Microbiological Culture Collection Center, with the preservation number of CGMCC No. 28398. The strain has strong acid resistance, can adapt to the digestive tract environment, and has high bile salt hydrolase activity. At the same time, in animal experiments, the strain significantly reduces blood glucose and serum cholesterol levels, reduces liver fat deposition, reduces adipose tissue inflammation, effectively reduces the weight, and has broad application prospects in the development of products for improving obesity, lowering lipid and lowering blood glucose.

The negative pressure liposuction device comprises a machine shell, a liquid storage pipe and a collecting pipe are installed at the bottom of the side face of the machine shell, a liquid injection opening is formed in the surface of the machine shell, a suction pump is installed at the back end of the machine shell, a connecting pipe is connected between the suction pump and a cover plate at the top end of the collecting pipe, and a top plate slides in the liquid storage pipe. According to the device, through the arrangement of the top plate, the adjusting pipe, the electric push rod and the suction force adjusting mechanism, the suction force in an area with more or less fat is adjusted according to the input amount of the swelling liquid, and fat tissues can be separated and liquefied more effectively by increasing the input amount of the swelling liquid in the area with more fat; the suction process is smoother, fat can be sucked more quickly through large suction force, the operation time is shortened, and the risk of injury to subcutaneous tissue can be reduced by reducing the input amount of swelling liquid and the suction force in an area with the small fat amount.

The invention discloses application of caffeic acid phenethyl ester to improvement of negative effects of high-carbohydrate feed on fish growth and metabolism, and belongs to the technical field of aquaculture. The caffeic acid phenethyl ester is added into a high-carbohydrate feed formula, so that the metabolic capability of adipose tissues, livers and muscles of the grass carp and the weever on glucose can be remarkably enhanced, ectopic deposition of lipid in the livers and the muscles is relieved, fatty liver diseases are improved, muscle protein deposition is promoted, and the immunity of the grass carp and the weever is improved. The inhibiting effect of the high-carbohydrate feed on the growth performance of the grass carp and the weever is reversed. The invention further provides formulas of the high-carbohydrate feed containing the caffeic acid phenethyl ester for the grass carp and the weever respectively, and data support is provided for research on improvement of the utilization capacity of the grass carp and the weever on the high-carbohydrate feed.

Embodiments provide device for treating sleep apnea, the device including: a stand, a chin and neck support, an applicator integrated into the chin and neck support configured to provide High-Intensity Focused Electromagnetic Technology (HIFEM) therapy and Radio Frequency (RF) therapy, and a control panel configured to adjust an intensity of the delivered HIFEM and RF therapies in real-time. The applicator is configured to provide HIFEM therapy by delivering HIFEM energy to a patient's mouth, throat, and neck to stimulate and strengthen muscles associated with the patient's breathing, and further configured to provide RF therapy by delivering RF energy to reduce fat tissue in the patient's airway.

The present invention relates to a method for in vitro method for diagnosing a cognitive impairment in a subject, or to in vitro method for monitoring the response to a therapy directed to improve the cognitive function in a subject suffering from cognitive impairment, comprising determining the expression levels of a gene specifically expressed in adipose tissue. The invention also discloses a kit to into practice the methods of the invention as well as a composition for use in the treatment of cognitive impairment by targeting the altered expression of this gene in adipose tissue.

Use of Mycobacterium vaccae, for example strain ATCC 15483, for the treatment or prevention of weight gain, visceral adipose tissue (VAT), obesity, obesity-related metabolic diseases and stress-related disorders.

The invention discloses a culture medium for adipose-derived stem cells, and particularly relates to the technical field of stem cell culture. The culture medium comprises a basic culture medium and nerve-vascular niche components, wherein the basic culture medium comprises DMEM / F12, 10% of human-derived platelet lysate and 1% of antibiotic solution; the nerve-vascular niche comprises the following components: a Slit3 recombinant protein, a nerve growth factor-beta, a glial cell-derived neurotrophic factor, a lysyl oxidase inhibitor, dexamethasone, 3-isobutyl-1-methylxanthine and insulin. The traditional fetal calf serum is replaced by the human-derived platelet lysate, so that the risks of immunological rejection and pathogen pollution are avoided; through the synergistic effect of nerve-vessel niche factors, blood vessel maturation and innervation are promoted, programmed mechanical stretching culture is combined, the adipogenic differentiation efficiency of adipose-derived stem cells is remarkably improved, and engineered adipose tissue which is huge in lipid droplet, compact in structure and provided with a nerve-vessel network is formed.