403 results about "Adipose tissue" patented technology

The invention provides a preparation method of cereal fermentation broth postbiotics and application of the cereal fermentation broth postbiotics in improvement of systemic chronic inflammation and reduction of cholesterol and low-density lipoprotein, and belongs to the technical field of fermentation engineering. The invention provides a preparation method of cereal fermentation broth metabiotics, which is characterized in that mixed cereals are used as raw materials, anaerobic fermentation is carried out by using compound enzymes and various bacteria, and polysaccharides and proteins in the cereal raw materials can be quickly converted into functional small molecular substances, such as polypeptides, total phenols and total flavonoids. The cereal fermentation broth metagen can inhibit secretion of a macrophage proinflammatory medium, inhibit generation of inflammatory factors of liver, heart and intestinal tissues, and improve activation of adipose tissue macrophages related to body inflammation. After the cereal fermentation liquor is subjected to post-biogen treatment, glucose and lipid metabolism disorder can be effectively improved, liver function damage can be relieved, and the intestinal barrier function can be effectively improved.

The invention relates to the field of biomedical materials, and particularly discloses a combined biovalve material anti-calcification method and a biovalve material. A combined bioprosthetic valve material anti-calcification method comprises the following steps: taking an animal-derived biological material, cleaning, removing adipose tissue, cutting a precordial region part to obtain a pericardium material, and storing the pericardium material in ice normal saline for later use; mixing the pericardium material and the decellularized treatment fluid A, oscillating and cleaning; adding the pericardium material into the decellularized treatment solution B, oscillating and cleaning; carrying out crosslinking treatment by utilizing a glutaraldehyde pericardium material; adding the crosslinked pericardium material into a reducing agent solution, oscillating and cleaning; the method comprises the following steps: putting a pericardium material into a glycerol-ethanol mixed solution or a glycerol-isopropanol mixed solution, and soaking for more than 24 hours; according to the anti-calcification method, on the basis that the mechanical property of the valve material is effectively maintained, the anti-calcification performance is improved, and the durability of the valve material is improved.

The invention relates to a regenerated decellularized adipose derivative as well as a preparation method and application thereof. The preparation raw material of the regenerated decellularized adipose derivative is prepared from a vascular matrix component (SVF) separated from human adipose tissue through decellularization treatment and freeze drying; the prepared regenerated decellularized adipose derivative is a porous material, the porosity is greater than 90%, and the aperture is 50-200 [mu] m; the composition comprises the following components in percentage by weight: 60-70% of collagen, 15-20% of elastin, 1 * 10 < 10 >-5 * 10 < 10 > particles / g of exosome, less than or equal to 5 ng / mg of DNA (deoxyribonucleic acid) residue, more than or equal to 30 ng / g of VEGF (vascular endothelial growth factor) and more than or equal to 15 ng / g of FGF-2 (fibroblast growth factor). The prepared regenerated decellularized adipose derivative is low in DNA residual quantity, rich in growth factors and capable of meeting the requirement for deep tissue filling due to the fact that the collagen content and the elastin content are high.

The invention provides a method for constructing a CT-MRI (Computed Tomography-Magnetic Resonance Imaging) personalized three-dimensional heart model based on multi-modal imaging, which comprises the following steps of: firstly, carrying out myocardial and infarction region segmentation on a CMR-LGE image, and carrying out myocardial and intramyocardial adipose tissue segmentation on a CE-CT image to generate a corresponding Label image; then, a high-precision three-dimensional volume mesh model of the corresponding ventricle is constructed based on the segmentation results of the two types of images; and then, mapping the corresponding Label image with the tissue type to a corresponding finite element model according to the coordinates of the grid, and obtaining a corresponding personalized ventricular model with the tissue type. An interpolation method based on a general ventricular coordinate system is adopted, data of an MR ventricular model with infarct tissue attributes is transferred to a CT ventricular model, and therefore a mixed CT-MRI ventricular model is constructed. And finally, storing the constructed CT-MRI ventricular model data with the integrated tissue attributes as a standard geometric and topological file format.

The invention relates to the field of microorganism sampling detection, in particular to escherichia coli sampling detection equipment for mouse experiments. The technical problem to be solved is that when mouse intestinal escherichia coli is sampled and detected, attention needs to be highly concentrated during manual operation, physical and mental exhaustion is easily caused, and the intestinal tract is damaged by slight carelessness. In addition, in the prior art, adipose tissue existing on the outer surface of the intestinal tract cannot be removed, and therefore the sampling detection result is affected. According to the technical scheme, the escherichia coli sampling and detecting equipment for the mouse experiment comprises a sampling box, a fixing assembly and the like; and a fixing assembly is mounted in the sampling box. According to the device, the left end and the right end of the intestinal tract of a mouse are fixed through the first clamping block and the second clamping block respectively, sterile water is injected into the intestinal tract of the mouse through an injection pipe for flushing, and cleaning of the intestinal tract of the mouse is completed, so that the pressure of manual operation is relieved, and meanwhile accidental damage to the intestinal tract of the mouse caused by manual operation is reduced.

The invention relates to brevibacillus parabrevis and application thereof in tissue isolation, and belongs to the technical field of biology. The brevibacillus parabrevis JN-6 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation number is CGMCC NO.29951, and the preservation date is March 7, 2024. The brevibacillus parabrevis JN-6 disclosed by the invention has the advantages that the preservation number is CGMCC NO.29951; the brevibacillus parabrevis provided by the invention has collagen degradation capability, and the produced collagenase can be used for adipose tissue separation and is further cultured into adipose-derived stem cells. When the produced collagenase is used for digestion and separation, the number of obtained cells can be 6.17 * 10 < 6 > / mL, the adipose-derived stem cells can be effectively differentiated, and the method has a good application prospect.

Described are systems and methods to determine one or more regional body composition values for one or more regions of a body based on a processing of one or more two-dimensional images that include a representation of the body. Regional body composition values may include any of a fat tissue mass, a lean muscle mass, a bone mineral density, a skeletal muscle mass, etc., which may be determined for any one or more regions of the body, such as the right arm, left arm, right leg, left leg, trunk, android, gynoid, etc.

The invention relates to an injectable acellular fat matrix composition as well as a preparation method and application thereof. Specifically, the invention provides an injectable decellularized fat matrix composition prepared by a high-pressure homogenization-enzymolysis coupling method, and the method comprises the following steps: performing high-pressure homogenization on a decellularized fat matrix to obtain matrix particles, performing protease enzymolysis, and performing freeze drying to obtain the injectable decellularized fat matrix composition. Therefore, the acellular fat matrix composition with excellent injectability, biological activity retention rate and long-term skin thickening effect is prepared.

Use of Mycobacterium vaccae, for example strain ATCC 15483, for the treatment or prevention of weight gain, visceral adipose tissue (VAT), obesity, obesity-related metabolic diseases and stress-related disorders.

The invention discloses a culture medium for adipose-derived stem cells, and particularly relates to the technical field of stem cell culture. The culture medium comprises a basic culture medium and nerve-vascular niche components, wherein the basic culture medium comprises DMEM / F12, 10% of human-derived platelet lysate and 1% of antibiotic solution; the nerve-vascular niche comprises the following components: a Slit3 recombinant protein, a nerve growth factor-beta, a glial cell-derived neurotrophic factor, a lysyl oxidase inhibitor, dexamethasone, 3-isobutyl-1-methylxanthine and insulin. The traditional fetal calf serum is replaced by the human-derived platelet lysate, so that the risks of immunological rejection and pathogen pollution are avoided; through the synergistic effect of nerve-vessel niche factors, blood vessel maturation and innervation are promoted, programmed mechanical stretching culture is combined, the adipogenic differentiation efficiency of adipose-derived stem cells is remarkably improved, and engineered adipose tissue which is huge in lipid droplet, compact in structure and provided with a nerve-vessel network is formed.

The application discloses a construction method of a fat-targeted gene therapy vector. The method comprises the following steps: obtaining a fat tissue-specific promoter sequence; obtaining a shRNA coding sequence targeting a Tks4 gene; cloning the promoter sequence and the shRNA coding sequence into an adeno-associated virus vector skeleton to construct a recombinant expression vector; and co-transfecting the recombinant expression vector and an auxiliary plasmid into a packaging cell to package and purify a recombinant adeno-associated virus particle. The vector constructed by the application adopts an adiponectin promoter to drive the expression of miR30-shRNA targeting the Tks4, and can be specifically expressed in fat tissues and livers after being injected through a tail vein, significantly inhibits fat cell hypertrophy and systemic fat accumulation induced by a high-fat diet, reduces the serum cholesterol level, does not cause an immune inflammatory reaction, does not affect sugar metabolism, and is not expressed in non-target tissues. The application provides a safe and efficient new strategy for the gene therapy of obesity, hyperlipidemia, fatty liver and related metabolic diseases.

The utility model provides a ruminant living body muscle adipose tissue minimally invasive sampling device, which relates to the technical field of animal tissue sampling, and comprises an outer sleeve and an inner cutting rod, one end of the outer sleeve is provided with a needle, the other end of the outer sleeve is provided with a handle, and the outer sleeve is provided with a sampling groove close to the needle; the inner cutting rod is movably arranged in the outer sleeve, and a sampling knife is correspondingly arranged at the sampling groove. The animal sampling device has the advantages of being high in sampling efficiency, capable of reducing animal injuries, convenient, easy and fast to operate and low in cost.

A method of preparing viable stromal and mesenchymal stem cells from adipose tissue that produces high quality and high counts of stem cells with a low risk of contamination. The apparatus provides ultrasonic waves through a constant temperature bath to the tissue held in a sterile sonication container such as a test tube or jar. No sonication probe touches the tissue or the cells during the process. The stem cells produced are ready to be administered to a patient.

The invention provides a preserving fluid for preserving adipose tissue-derived stem cell extracellular vesicles and a formula of a corresponding preparation, and particularly, the formula comprises sodium chloride, cane sugar, polysorbate 80, histidine and histidine hydrochloride. According to the formula, the storage stability of the extracellular vesicles secreted by adipose tissue can be improved, substances in the extracellular vesicles are prevented from being degraded, convenience is provided for clinical application of the extracellular vesicles, and good market prospects are achieved.

Disclosed are a method for creating a surgical training model, a surgical training model apparatus, a bone model, an article that emulates tissue of an animal musculoskeletal system, an article that emulates animal fat tissue, and an article that emulates animal skin tissue. One version of the method comprises placing a spinal vertebrae model in a cavity model that emulates an animal body cavity; forming a first layer on top of the vertebrae model, wherein the first layer emulates an animal muscle tissue; placing a second layer over the first layer, wherein the second layer emulates an animal fat tissue; and placing a third layer over the second layer, wherein the third layer emulates an animal skin tissue. The spinal vertebrae model can be 3D printed from a thermoplastic polymer and infiltrated with a foam into an interior space of the 3D printed spinal vertebrae model.

To provide a tissue product, more specifically, an extracellular tissue matrix made from adipose tissue.SOLUTION: A tissue product is provided, wherein the tissue product comprises a breast implant in the form of a porous acellular tissue matrix sponge, wherein the implant comprises an acellular adipose tissue matrix construct comprising a particulate acellular adipose tissue matrix, wherein the matrix is in a suspended, dried and stabilized form, and wherein the suspension comprises 2-10 wt.% solids.SELECTED DRAWING: None

The invention relates to the technical field of biological medicines, and discloses application of INHBC as a target spot in prevention and / or treatment of metabolic diseases. Specifically, the invention relates to application of substances for promoting INHBC gene expression or improving protein activity in preparation of products for preventing and / or treating metabolic diseases, and the metabolic diseases comprise diseases caused by energy metabolism disorder, such as obesity, diabetes, fatty liver disease, hyperlipidemia and the like. According to the application disclosed by the invention, liver specific overexpression or INHBC knock-in can improve mouse liver fatty degeneration and abnormal glucose metabolism induced by high fat diet, and in addition, the heat production activity of mouse adipose tissues can be enhanced, so that obesity induced by high fat diet is inhibited; the compound has an important application value in preventing and / or treating metabolic diseases such as obesity, type 2 diabetes and fatty liver diseases.

The invention discloses an adipose tissue obtaining needle capable of reducing bleeding and nerve injury, which comprises a guide rod, one end of the guide rod is fixedly communicated with a negative pressure suction bottle connecting assembly, the other end of the guide rod is fixedly communicated with a needle head, and the guide rod is provided with a plurality of water drop holes close to the needle head. A user cannot be stabbed when being accidentally touched, the sharp and solid round blunt needle penetrates through a collection part to reduce the resistance of a traditional blunt end, the phenomenon that the cortex of the collection part is damaged is avoided, and the strain of the collection part is reduced; the water drop holes are formed, stress is better dispersed, the tensile strength of the guide rod during fat collection is improved, the service life is prolonged, and compared with a traditional solid guide rod and a guide rod with the water drop holes, normal use is guaranteed, meanwhile, the weight of the guide rod is reduced, and positioning and transferring are convenient; and by arranging the guide groove, the problems that the needle deviates from the fat collecting direction, fat extraction is not uniform, and even the fat extraction position deviates are solved.

The invention relates to a regenerated fat derivative as well as a preparation method and application thereof. According to the preparation method, the fat derivative freeze-dried powder rich in various cytokines, collagen and hyaluronic acid can be obtained from human adipose tissues. The regenerated fat derivative has the advantages of being good in structural stability, not prone to degradation, stable in form, good in safety and the like, and can be used for preparing medical products or cosmetic products. The medical products comprise repair dressings, injections, hemostatic gel, hemostatic filling gel, hemostatic materials or filling microspheres, and the beauty products comprise facial masks, nano-liposome essence or cream water emulsion spray.

The present invention discloses a biopsy forceps guiding catheter that can be used for zero-ray myocardial biopsy, comprising a catheter body, wherein a central cavity penetrating through both axial ends of the catheter body is provided inside the catheter body, at least one electrode element is provided at the first axial end of the catheter body, an airbag component is provided at the first axial end of the catheter body near the rear side of the electrode element, the electrode element is connected to an electrical connection assembly extending to the second axial end of the catheter body, the electrical connection assembly is connected to an electrocardiogram monitor, and the airbag component is connected to a ventilation assembly extending to the second axial end of the catheter body. The electrocardiogram and injury current in the cardiac cavity are recorded by the electrocardiogram monitor leads to select a site with a significant injury current for sampling, thereby reducing the influence of scars and fat tissue on the quality of the sampled sample, and the airbag component can drift and move with the blood, thereby solving the problem that the current biopsy forceps need to rely on X-ray guidance to be delivered into the ventricle and it is difficult to accurately obtain a specific layer of myocardium under the endocardium during sampling.

Devices, systems, and methods for treatment of the subcutaneous tissues of various types. Non-invasive ultrasound energy is used for planning, monitoring and treating the adipose tissue, small blood vessels, and benign tumors of the subcutaneous tissue. While high intensity focal ultrasound (HIFU) has been used to treat many areas of the body including cancer lesions typically using ablation / high heat energy, lower intensity volumetric ultrasound (LIVU) techniques are used to denature the subcutaneous layer. Various safety features may be included to further enhance the treatment of the subcutaneous tissue using LIVU techniques.

The present invention discloses a thiophene-substituted aza-fluoro-boron-dipyrrole compound, nanoparticles of the compound, and their preparation methods and applications. The thiophene-substituted aza-fluoro-boron-dipyrrole compound designed and synthesized by the present invention, the introduction of the thiophene group can significantly red-shift the absorption spectrum of aza-fluoro-boron-dipyrrole, successfully red-shifting to the 810nm near-infrared region, which is more conducive to its application in vivo and in photothermal therapy. Nanoparticles prepared with this compound as the core can be effectively distributed to visceral adipose tissue after local intraperitoneal administration, selectively targeting visceral adipose tissue, have good biocompatibility and high photothermal conversion efficiency, can achieve temperature regulation by adjusting its concentration and 808nm laser power, and can be used for photothermal treatment of obesity.

The invention provides application of loranthus parasiticus in preparation of a lipid-lowering medicine. The application has the advantages that the loranthus parasiticus extract can effectively improve HFD-induced obesity and glucose and lipid metabolism disorder; the loranthus parasiticus extract can relieve adipose tissue enlargement and liver fatty degeneration.

The present invention relates to a Korean microbiome-derived Clostridium butyricum strain having anti-obesity efficacy and a use thereof. The strain has an excellent effect of reducing the body weight and adipose tissue weight of experimental animals compared to a control group (strain-untreated) under high-fat diet conditions, has an excellent effect of reducing the content of total cholesterol, triglycerides, low-density lipoprotein (LDL), leptin, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in serum, and has an excellent effect of reducing blood glucose and increasing the content of glucagon-like peptide-1 (GLP-1) in an oral glucose load test, and is thus expected to be effectively used as an ingredient in a composition for preventing, alleviating, or treating obesity and metabolic diseases caused by obesity.

The purpose of the present invention is to provide an excellent infertility treatment capable of improving the implantation rate of an embryo (fertilized egg) by improving the uterus environment. The present invention is a composition for treating uterus, which contains a mesenchymal stem cell culture supernatant, and which is used as a uterus environment-improving agent, an implantation aid, an embryo transplantation solution, a sperm transplantation solution or an implantation rate-improving agent. The mesenchymal stem cells are preferably derived from adipose tissue, umbilical cord tissue or bone marrow tissue.

The present invention relates to an adipose tissue fluidification device, specifically an adipose tissue fluidification device for obtaining nanofat for reinjection into a patient as a facial or body filler, comprising a body, two ends aligned along the same geometric axis, and a plurality of channels internal to the body or three meshes internal to the body.

This invention provides tissue products manufactured from adipose tissue and methods for manufacturing the same. [Solution] A method for producing a tissue product is provided, comprising the steps of: selecting adipose tissue; processing the tissue to remove substantially all cellular material from the tissue; suspending the tissue in a liquid to form a suspension containing 2-4% by weight of solids; and freezing and drying the suspension to form a porous sponge.

The invention relates to the technical field of biomedical materials and tissue engineering, and discloses a preparation method and application of adjustable porous fat foam. Comprising the following steps: fat tissue pretreatment and freeze-thaw circulation, multi-step combined decellularization and impurity removal, graded washing and purification, freeze grinding and freeze drying, preparation of a decellularized fat matrix suspension, porous foam scaffold forming and foam scaffold gradient rehydration. By adopting a multi-step combined decellularization and impurity removal process, residual lipid droplets in adipose tissues can be actively extruded out by mechanical squeezing assisted by isopropanol degreasing, a lipid wrapping phenomenon is broken, meanwhile, residual nucleic acid and adipose components can be thoroughly removed by virtue of a synergistic degradation effect of nuclease and lipase, the immunogenicity risk is reduced, and the immunogenicity of the adipose tissues is improved. All the steps have a synergistic effect under strict control of temperature, concentration and time parameters, so that the structural integrity of the extracellular matrix is ensured, the decellularization efficiency and thoroughness are improved, and the biological safety and biocompatibility of a final product are ensured.

The present invention refers to a delivery system for molecules, preferably drugs, said system comprising fat tissue or derivatives thereof. Moreover, the present invention refers to a fat-based delivery system, preferably loaded with molecules having antitumor activities, for use in the treatment of cancers.

The invention belongs to the technical field of biology, and particularly relates to application of a herba artemisiae scopariae aqueous extract in enhancing liver chemotaxis of adipose-derived mesenchymal stem cells. In order to provide a way for enhancing the liver chemotaxis of the adipose-derived stem cells, the capillary artemisia aqueous extract is used for improving the expression level of chemotactic factors of the adipose-derived stem cells in vitro and intervening the proliferation, migration and chemotaxis capabilities of the adipose-derived stem cells to liver cells, the concentration of the capillary artemisia aqueous extract is 0.5 mg / mL to 1 mg / mL, the chemotaxis time is 24 h to 48 h, and the capillary artemisia aqueous extract is used for enhancing the liver chemotaxis of the adipose-derived stem cells. And the chemotactic factors are CCR1 and CXCR4.