61 results about "Cecum" patented technology

A temporary ostomy appliance is disclosed, including a catheter for extending through the abdominal wall into the intestine. The catheter may be a transcecal catheter for extending through the cecal valve into the ileum. A portion of a catheter that extends through the cecal valve is made collapsible when the catheter is empty. The collapsed portion expands to permit passage of effluent. A balloon carried on the catheter is preformed with a shape and size in order to permit inflation without elastic stretching of the balloon wall material. A filament is provided for permitting a portion of the catheter to be fastened to internal body tissue by surgical sutures or staples. In order to release the fastening without further surgery, the filament is withdrawn by pulling on a proximal portion outside the body.

Method for draining and decompressing a colon in which a drainage tube is inserted through the anus into the colon to extend through the entire colon. The drainage tube is fixed in position and suction is applied therethrough to cause fecal matter to pass therein through apertures and be withdrawn from the colon. The drainage tube is inserted by inserting a guidewire into the colon through the anus, manipulating and pushing the guidewire to the cecum, passing a filament through the colon wall to engage the guidewire, pulling the guidewire out of the colon and anus to draw the filament through the colon and out of the anus, attaching the drainage tube to the filament and drawing the filament with attached drainage tube through the anus into the colon. The invention also relates to constructions of the guidewire, the drainage tube and methods for inserting drainage tubes using guidewires.

Method for draining and decompressing a colon in which a drainage tube is inserted through the anus into the colon to extend through the entire colon. The drainage tube is fixed in position and suction is applied therethrough to cause fecal matter to pass therein through apertures and be withdrawn from the colon. The drainage tube is inserted by inserting a guidewire into the colon through the anus, manipulating and pushing the guidewire to the cecum, passing a filament through the colon wall to engage the guidewire, pulling the guidewire out of the colon and anus to draw the filament through the colon and out of the anus, attaching the drainage tube to the filament and drawing the filament with attached drainage tube through the anus into the colon. The invention also relates to constructions of the guidewire, the drainage tube and methods for inserting drainage tubes using guidewires.

The invention relates to a formulation for the delayed and controlled delivery of an adsorbent into the lower intestine of mammals. The formulation includes a carrageenan and an adsorbent, such as activated charcoal. The invention further relates to uses of this formulation, in particular to pharmaceutical uses. In one embodiment, the formulation is used to eliminate or reduce the side effects in the intestine, in particular in the colon, of pharmaceutical agents that are administered as a treatment for a disorder, but that have side effects when they reach the late ileum, the caecum or the colon.

Apparatus and methods for extending a tube, the apparatus including a foldable tube having a folded portion inside an unfolded portion of the tube, and a source of fluid coupled to the unfolded portion for providing fluid into the unfolded portion to unfold and extend the folded portion out of the unfolded portion to become an extension of the unfolded portion. The apparatus and methods are useful, inter alia, for cleansing the colon in the normal direction, that is, from the cecum to the anus, using an apparatus that is introduced via the anus. A washing liquid is supplied deep into the colon via the soft feed tube that is inserted via the anus and is extended into the colon by inflation. The washing liquid is then drained out via the anus through a drainage channel.

The invention relates to a lactobacillus reuteri lyophilized preparation and a preparing method thereof, belonging to a microbial feedstuff preparation additive; the lactobacillus reuteri lyophilized preparation is characterized in that the weight percentage of the protectant of the lyophilized preparation is that 1 percent of sucrose, 12 percent of skimmed milk, 4 percent of lactose, 1 percent of glycerin, 2 percent of starch and the rest of water; the lactobacillus reuteri lyophilized preparation of the invention is separated by the mucous membrane of cecum of soy sauce chicken, and the lactobacillus reuteri is identified by Chinese industrial microbial culture collection management center; the bacterial strain of the invention is fermented and enlarged, and then is frozen and dried; the viable count of the freezing additive reaches 10<10>cfu / g, and can be used for feeding animals directly; the experiment of the safety of animals and the experiment of feeding the animals prove that the bacterial strain has higher safety and functionality.

The invention discloses an RPA (recombinase polymerase amplification) primer for detecting a fowl adenovirus serotype 4. The nucleotide sequence of the RPA primer is as shown in SEQ ID No.1 and SEQ ID No.2 in the description. The invention further discloses a method for detecting the RPA of the fowl adenovirus serotype 4, and the method mainly comprises the steps of primer synthesis, extraction of DNA in a sample to be tested, RPA amplification reaction and analysis of an RPA amplification product. According to the RPA primer disclosed by the invention, the specificity is strong, the sensitivity is high, and the detection result is accurate. The detection method disclosed by the invention is simple in operation and good in stability, and a low-cost, rapid and specific on-site diagnosis method for effectively detecting and authenticating chicken cecum hepatitis-hydropericardium syndrome is provided.

The invention is intended for secure emptying of organism's cavities, particularly for colon decompression. The device combine the drain with means of its delivery in the shape of everting by fluid invaginator, comprising the drain, as well as their intractor. The secure emptying of cavity is ensured by a hose with punctures, placed in drain. The liquid, feeding into hose, realizes drain's recanalization, irrigates its holes and dilutes cavity contents. Transportation of drain into colon is ensured by synergy of two intubation forces. The vanguard force is generated by fluid pressure which everts invaginator, the rearguard (extraanal)—by “cylinder-piston” unit with a cuff. The hollow unit's piston acts as pneumatic carriage, but cuff is periodically joining with invaginator with drain or intractor. Evacuaton of contents is ensured by influx-and-extract method. The sequential connection of intractor and drain ensures not only intubation, but also contents' evacuation through both ends of drain. On the drawing is represented cecum with a distal fragment of drain-irrigation system of device: at left—in “evacuation” mode, at right—in “drain's recanalizaton-irrigation”, “intestines irrigation” mode.

Techniques, methods and lavages are disclosed for prevention or treatment of shock, particularly cecal ligation or cecal inoculation shock, by administering a specific therapeutic agent, which is able to use smaller volumes of reagent to achieve partial to complete inhibition, than other previously described techniques. The agent includes a combination of enzyme inhibitor, cytotoxic lipid binding protein, and antibiotic.

Preparations for preventing bile acid diarrhea characterized by containing a bile acid adsorbent coated with a polymer so as to allow the release thereof around the lower part of the small intestine and the cecum; and medicines (for example, antilipemics) comprising a combination of bile acid re-absorption inhibitors with the above-described preparations for preventing bile acid diarrhea.

The invention provides an application of codonopsis pilosula polysaccharide as medicine for regulating intestinal flora of human body, namely the application of codonopsis pilosula polysaccharide as medicine, food or health products for preventing colitis. An acute colitis mouse model is established by using fructo-oligosaccharide, codonopsis pilosula polysaccharide and codonopsis pilosula saponinto be respectively prevented in a healthy mouse model for 21 days and adopting 3% dextran sodium sulfate to be induced for 7 days. The remission of three drugs to macroscopical pathological symptomsof colitis is compared by evaluating changes of body weight, colon length, pathological index of disease, histological section and myeloperoxidase activity of experimental mice and the like. The regulation of drug on intestinal flora of colitis mice is investigated by using the Illumina MiSeq 250 sequencing platform based on the variable region of 16S V3-V4, and dominant bacteria in each drug intervention group are identified, which shows the absorption of the highest exposure amount of monomer saponin in codonopsis pilosula saponin in serum and the cecum, clarifies the mechanism that codonopsis pilosula polysaccharide plays a role in the treatment of diseases by changing the action of intestinal flora on saponin metabolism.

The invention discloses a double-balloon intestine fistulization catheter for preventing anastomotic fistula after a colorectal surgery. The double-balloon intestine fistulization catheter comprises a catheter body, a first balloon, a second balloon, a threaded compressed tablet, a first water injection opening and a second water injection opening, wherein the first balloon and the second balloon are two hollow balloons which sleeve the catheter body and are made of an elastic material; the first balloon is close to the head end; the threaded compressed tablet sleeves the part between the second balloon and the tail end; a side hole is formed in the wall, between the first balloon and the head end, of the catheter body; the first water injection opening and the second water injection opening are positioned at the tail end of the catheter body, and are respectively communicated with the first balloon and the second balloon. According to the invention, when the catheter is inserted into the intestinal tract after an intestinal surgery, the circulation of the small intestine is blocked after the first balloon is injected with water to enable intestinal fluid to be led out of the body through the head end and side hole of the catheter; after being injected with water, the second balloon and the threaded compressed tablet positioned outside the body are coordinated to fix the catheter to prevent slipping or shifting of the catheter, and at the same time, the intestinal wall of the cecum is clung to and adhered with the abdominal wall to prevent intestinal fluid from leaking outwards to the abdominal cavity.

The invention discloses a diet starch source combination capable of improving health of intestinal tracts of piglets. The ratio of amylose to amylopectin is 0.48, so that the concentration of total short chain fatty acids, particularly the concentration and the molar ratio of butyrate, of contents in cecum and colon of a piglet can be increased, and accordingly maintaining of completeness and functions of enterocyte of hindgut of the piglet is facilitated. After the piglet is fed by feed containing the amylose and the amylopectin with the ratio of 0.48 for a long time, the gastro-intestinal morphology and the intestinal enzyme activity of the piglet can be improved, the height of intestinal villus is increased, the depth of a crypt is increased, accordingly, growth and development of the small intestine of the piglet can be promoted, and the gastro-intestinal morphology structure is improved. As a starch source with the ratio of the amylose to amylopectin to be 0.48 is used as a daily feed energy source, health of the intestinal tracts of the piglet is possibly facilitated, and development of later-period production performance of the piglet is also facilitated.

The invention relates to the field of biological analysis methods, and in particular to an analysis method of cecal metabolome in a broiler chicken. The change of metabolites and metabolic pathways ofcecal contents after the broiler chicken eats a yeast culture produced at different fermentation time is detected by jointly using a gas chromatography-mass spectrometry, thereby determining the influence of the yeast culture on the cecal metabolic regulation and the metabolites of the broiler chicken. Metabolomics data show that yeast culture is used for feeding the broiler chicken, the cecal differential metabolites mainly involve in amino acid metabolism and cholesterol metabolism, and participates in fat, vitamin digestion and absorption processes.

The invention provides a preparation method of a mice intestinal flora alteration ERIC-PCR (Enterobacterial Repetitive Intergenic Consensus sequence-based Polymerase Chain Reaction) fingerprint spectrum. The preparation method comprises the steps: moulding mice intestinal flora alteration caused by antibacterial-associated diarrhea, extracting a cecum content DNA, eliminating humic acid in the cecum content DNA, eliminating a PCR inhibitor in the cecum content DNA, performing ERIC-PCR amplification and native polyacrylamide gel electrophoresis (Native-PAGE), and comparing with a conventional flora analysis result so as to establish the ERIC-PCR fingerprint spectrum of the mice intestinal flora alteration. According to the preparation method, with a pure-series mice as a research object, the defect caused when a conventional bacterium culturing method is applied to the microflora analysis is solved. The preparation method can be used for rapid detection of the laboratory mice intestinal flora alteration, prevents the defects of complicated operation, long time consumption, large workload and the like of conventional bacterium separation and culturing, and is capable of accurately, rapidly and effectively detecting the mice intestinal flora distribution condition.

The invention relates to a lactobacillus plantarum RS-09 with high adhesion and applications thereof, and belongs to the field of microorganisms. The strain is separated from apple juice, and is preserved in in the China General Microbiological Culture Collection Center with a preservation number of CGMCC NO.17118; has stronger acid resistance and choline salt resistance, can be stored for a longtime, has higher adhesion capacity to human colon cancer cells HT-29, and can be stably planted in intestinal tracts, wherein the planting rate of the cecum is highest; can obviously reduce the content of TNF-alpha in serum, increase the content of IL-10 and IL-4 in the serum, and has the function of immunoregulation; and has strong bacteriostatic effect on salmonella enteritis, has prevention andtreatment effects on enteritis diseases caused by flora disorder, can be applied to the field of functional foods, not only has practical production value, but also has very important significance onbody health.

Provided herein are compositions, systems, and methods for treating a disease, such as kidney and / or cardiovascular disease, with an agent that reduces the production of trimethylamine (TMA) or trimethylamine-n-oxide (TMAO) in a subject. In certain embodiments, the agent is: i) 3,3-dimethyl-1-butanol (DMB) or a DMB derivative or related compound, ii) acetylsalicylic acid or derivative thereof (e.g., with an enteric coating for delivery to the colon and / or cecum); iii) a flavin monooxygenase 3 (FMO3) inhibitor; iv) a gut TMA lyase inhibitor; v) an antibiotic or antimicrobial; vi) a probiotic or prebiotic; vii) an antiplatelet agent; or viii) a TMA and / or TMAO sequestering agent.

This invention relates to new methods of treating parasitic infections, such as trichomonas vaginalis infection and cecum amoeba infection, using L-enantiomer enriched ornidazole, in particular enantiomerically pure L-ornidazole, which provides benefits such as higher efficacy and lower toxicity to central nervous system over the existing racemic Ornidazole drug. New methods of synthesizing L- and D-enantiomers of Ornidazole in high purity and enantiomeric excess (ee), new formulations of the enantiomerically enriched L- or D-ornidazole, as well as their preparation processes and methods of use, are also disclosed.

The present invention relates to the field of food and particularly relates to astaxanthin-enriched inulin solid drink and a preparation method thereof. The astaxanthin-enriched inulin solid drink comprises the following ingredients of an astaxanthin extract, inulin, a sweetener and compound fruit juice powder. The preparation method of the astaxanthin-enriched inulin solid drink comprises the following steps: S1, mixing is conducted: raw materials of the astaxanthin extract, inulin, sweetener and compound fruit juice powder are mixed to obtain the mixed raw materials; S2, granulating is conducted: the mixed raw materials are granulated using a boiling-type granulation tower; temperature is controlled at 50-55 DEG C; activity of each of the components is ensured; and the granulating time is 25-30 min; and S3, cooling is conducted: after the granulation, the granulated materials are placed and cooled to obtain a finished product. The inulin can generate butyrate by the fermentation in human body cecum (or colon) and enables crypts in mucosa to get shallow; cells in each crypt are increased to thus increase absorption areas; cecal veins are more developed, thereby promoting absorption effects of the astaxanthin in human body; the astaxanthin is prepared into the solid drink; and the sold drink is easy for consumption.

The invention relates to a method for establishing a model of fluorescence orthotopic transplantation of subcutaneous metastasis organ cancers, which is characterized in that human colon adenocarcinoma cell strains or cancer cell strain green fluorescent proteins (GFP) of corresponding organs are selected; GFP plasmids carry out transfection on cancer cell strains of the organs via retrovirus pLPCX so that the cancer cell strains of the organs contain GFP expression plasmids; a GFP gene is connected with a pLPCX vector via a T4 ligase; a GFP-pLPCX vector is transfected to a PT67 package cell to obtain a Lovo cell; nude mice are selected and subjected to subcutaneous inoculation with Lovo cell suspension; tumors can be formed in about 4 weeks; orthotopic transplantation is carried out after green fluorescence is observed; a cut is opened on the skin and peritoneum; about 1cm cecum of the nude mice is taken out of the peritoneum; the gap between the cecum and the peritoneum is sutured and the cecum is transferred subcutaneously; the cut cecum is subjected to serosa; two fluorescence-carrying tumors with diameter of about 1mm are transplanted to the cecum via the suture and the skin is closed.

The invention discloses an escherichia coli CG1061 strain for effectively degrading aflatoxin B1. The strain is collected in the Guangdong microbial culture collection center on February 1, 2018, withthe collection number being GDMCC NO:60324. The strain is separated from a chicken cecum, is a nonpathogenic escherichia coli, can effectively degrade AFB1, can be applied to detoxicating microbes, detoxicating enzymic preparations and the like for preparing aflatoxin B1, and has a good application prospect in the fields of food and feed processing and the like.

The invention provides a tank container train loading and unloading arm, and belongs to the technical field of tank container train loading and unloading equipment. The tank container train loading and unloading arm comprises a supporting mechanism, a lower arm tube, an inner arm tube, a middle arm tube, an outer arm tube, a hose, an upper arm tube, a quick joint and other parts. The tank container train loading and unloading arm has the beneficial effects that the bottom unloading of the end surface of a tank container train is realized under the condition that the train is not unlocked, thedesign of each arm tube meets the requirement that the dead point and cecum phenomenon is required not occur on a conveying pipeline when the heavy-mass oil such as fuel oil is loaded and unloaded, noliquid bag exists in the flowing direction of a medium in the whole process, and the oil discharging efficiency and the safety and reliability are greatly improved; and the upper arm tube, the metalhose, the outer arm tube, the middle arm tube, the inner arm tube, the lower arm tube, the rotary joint and the like cooperate in a collaborative mode so that the connecting port plane of the quick joint can be kept parallel to the connecting port plane of a liquid discharging port, and then the quick joint and the liquid discharging port can be smoothly, accurately and hermetically connected.

The invention discloses a gradient centrifugation separation method of total microbes in porcine gastrointestinal contents and manure. The gradient centrifugation separation method comprises the following steps: A, performing reagent preparation of a separation buffer solution; B, performing an experimental method as follows: (1) selecting the porcine gastrointestinal contents and manure and aseptically collecting contents and manure in stomach, jejunum, ileum, cecum and colon; (2) respectively weighing the weight of the contents and manure, placing into a sterile centrifugal tube, centrifuging and discarding supernatant; (3) adding the separation buffer solution into the centrifugal tube, shaking up on a vortex mixer and centrifuging; (4) taking out the centrifugal tube, pouring supernatant fluid into the centrifugal tube, centrifuging the centrifugal tube loaded with the supernatant fluid and discarding the supernatant; (5) weighing the separation buffer solution, adding into a centrifugal tube a, shaking up on the vortex mixer, centrifuging and discarding the supernatant; (6) respectively repeating the step 3, the step 4 and the step 5 twice; (7) pouring away the supernatant fluid, transferring thalli into a 2ml centrifugal tube and preserving at the temperature of -70 DEG C for determining the dry weight of the thalli and a microbial flora. The method is easy, simple and convenient to operate and the cost is low.

Oral drug delivery formulations which specifically administer antibacterial agents to the ileum, caecum, and / or the colon, without significant administration elsewhere in the gastrointestinal tract, are disclosed. The formulations include, as actives, a combination of a macrolide or aminoglysoside, or quinolone antibacterial and an anti-Gram-negative lipopeptide (polymyxin) antibacterial agent or other peptide antibacterials effective against Gram-negative bacteria. The formulations can be used to treat infections or unwanted colonization in the colon, and to provide effective decontamination of the colonic flora from unwanted or potentially pathogenic bacteria.

A temporary ostomy appliance is disclosed, including a catheter for extending through the abdominal wall into the intestine. The catheter may be a transcecal catheter for extending through the cecal valve into the ileum. A portion of a catheter that extends through the cecal valve is made collapsible when the catheter is empty. The collapsed portion expands to permit passage of effluent. A balloon carried on the catheter is preformed with a shape and size in order to permit inflation without elastic stretching of the balloon wall material. A filament is provided for permitting a portion of the catheter to be fastened to internal body tissue by surgical sutures or staples. In order to release the fastening without further surgery, the filament is withdrawn by pulling on a proximal portion outside the body.

The invention discloses an application of lactobacillus johnsonii CGMCC No.4926 in lowering a pH value of intestinal tract of a weaning piglet. Addition of proper dosage of the lactobacillus johnsonii to forage can lower pH values of ileum and cecum of the weaning piglet. A low pH value of the intestinal tract is not benefit for growth of intestinal tract pathogen, so that with the addition of the lactobacillus johnsonii, effect of prevention of diseases caused by the pathogen can be reached.

The invention relates to a lactobacillus reuteri lyophilized preparation and a preparing method thereof, belonging to a microbial feedstuff preparation additive; the lactobacillus reuteri lyophilized preparation is characterized in that the weight percentage of the protectant of the lyophilized preparation is that 1 percent of sucrose, 12 percent of skimmed milk, 4 percent of lactose, 1 percent of glycerin, 2 percent of starch and the rest of water; the lactobacillus reuteri lyophilized preparation of the invention is separated by the mucous membrane of cecum of soy sauce chicken, and the lactobacillus reuteri is identified by Chinese industrial microbial culture collection management center; the bacterial strain of the invention is fermented and enlarged, and then is frozen and dried; the viable count of the freezing additive reaches 10<10>cfu / g, and can be used for feeding animals directly; the experiment of the safety of animals and the experiment of feeding the animals prove that the bacterial strain has higher safety and functionality.

The invention relates to a composition capable of increasing yield of eimeria tenella coccidian oocysts and a preparation method thereof. The composition is composed of, by weight, 30-50 parts of cortex phellodendri, 0.5-5 parts of propolis, 20-60 parts of coptis chinensis and 10-20 parts of metronidazole. The composition is scientific and reasonable, simple in process, convenient to use and low in cost. The composition can increase yield of eimeria tenella coccidian oocysts through effects of preventing and treating intestinal inflammation, inhibiting breeding of anaerobic bacteria in cecum,promoting tissue cell regeneration and accelerating intestinal mucosa repair, reduce the number of chicks used for oocyst propagation and lower manpower, material resource and time cost, thereby greatly improving production efficiency of avian coccidiosis vaccines.

The invention discloses an identification method for campylobacter jejuni infection-associated chicken microRNA. The identification method comprises the following steps of: a first step of collecting chicken meconium, and detecting a positive rate of campylobacter jejuni; a second step of collecting cecum content for counting campylobacter jejuni and cecum tissue samples for RNA extraction; a third step of extracting total RNA of samples, detecting quality, and mixing in a tank; a fourth step of constructing a sequencing sample library; a fifth step of carrying out Solexa sequencing; a sixth step of carrying out subsequent analysis; a seventh step of predicating a target gene of the microRNA; an eighth step of obtaining campylobacter jejuni cfu infection-assocaited chicken microRNAs according to the microRNA and the target gene information thereof. The identification method disclosed by the invention regulates the target gene of the microRNA to indirectly participate in a plurality of immune-associated signal channels, so that important regulation effect is achieved in campylobacter jejuni infection; the microRNAs can be important candidate microRNAs in anti-campylobacter jejuni infection breeding, and foundation is laid up for deeply researching an acting mechanism of the microRNAs in campylobacter jejuni in future, so that theoretical basis and scientific basis are provided for molecular disease-resistant breeding of chicken.

The invention belongs to the technical field of biological medicine, and particularly relates to a medicine composition for preventing or treating pyohemia. The invention discloses application of Huperzine A (HupA) and (TIENAM) for preparing medicine for preventing or treating pyohemia through combined use, and medicine prepared by the method. The HupA and the TIENAM have the obvious treatment effect when being used in a combined way, so that the Cecal Ligation and Puncture (CLP) mouse survival rate is further improved to 50 percent (P is smaller than 0.05) from 15 percent; the expression of inflammatory factors in blood serum is inhibited to different degrees; the pathologic damage of the pyohemia on tissues of lungs, livers and the like can be obviously reduced; the organ failure is inhibited.