153 results about "Campylobacter jejuni" patented technology

The present invention demonstrated the potential use of Lactobacillus johnsonii D115 as a probiotic, as a prophylactic agent or as a surface treatment of materials against human and animal pathogens such as Brachyspira pilosicoli, Brachyspira hyodysenteriae, Shigella sonnei, Vibrio cholera, Vibrio parahaemolyticus, Campylobacter jejuni, Streptococcus pneumoniae, Enterococcus faecalis, Enterococcus faecium, Clostridium perfringens, Yersinia enterocolitica, Escherichia coli, Klebbsiella pneumoniae, Staphylococcus aureus, Salmonella spp., Bacillus cereus, Aspergillus niger and Fusarium chlamydosporum. The proteineous antimicrobial compound was partially characterized and found to be heat tolerant up to 121° C. for 15 min, and acid tolerant up to pH1 for 30 min at 40° C. The compound is also stable to enzymatic digestion, being able to retain more than 60% antimicrobial activity when treated with pepsin and trypsin.

The invention relates to lactobacillus plantarum CCFM8724 and application thereof. The lactobacillus plantarum CCFM8724 has ability of suppressing growth of campylobacter jejuni in vitro, good resistance to acid and cholate and good adhesive ability to intestinal epithelial cells, and can control the campylobacter jejuni adhering to the intestinal epithelial cells so as to suppress growth of the campylobacter jejuni in chicken. The lactobacillus plantarum CCFM8724 can be used for preparing a medicinal composition for suppressing campylobacter jejuni; a fermentation agent containing the strain of lactobacillus plantarum CCFM8724 can be used for producing fermented dairy products, fermented bean products, fermented fruit/vegetable products and silage; the fermentation agent enables the products to obtain certain acidity and particular flavor; and meanwhile, the product preserving time is prolonged, and the nutritional value, digestibility and safety of the products are improved.

The invention relates to a pathogenic microorganism DNA detection chip and the chip comprises a carrier and a nucleic acid probe on the carrier. The nucleic acid probe is provided with a target detection probe used for detecting target gene of pathogenic microorganism. The pathogenic microorganism comprises but not limits to vibrio cholerae, pathogenic escherichia coli, campylobacter jejuni, Yersinia enterocolitica, parahemolytic vibrio, salmonella, and shigella and Listeria monocytogenes. The invention also relates to a preparation method of the pathogenic microorganism DNA detection chip and provides applications of the pathogenic microorganism DNA detection chip in detection of pathogenic microorganism. Also a pathogenic microorganism DNA detection chip kit is provided.

The invention relates to the production of ganglioside specific antibodies. These antibodies are produced following immunization with lipopolysaccharide antigen of Campylobacter jejuni. The antibodies bind to monosialogangliosides, including GM2 and GM1.

The invention discloses a quadruple fluorescent PCR (Polymerase Chain Reaction) detection kit for common chicken food-borne bacteria and an application method thereof. The kit contains a fluorescent PCR solution and standards of Clostridium perfringens, Salmonella enteritidis, Escherichia coli O157: H7 and Campylobacter jejuni, wherein the fluorescent PCR solution contains Taq DNA (deoxyribonucleic acid) polymerase, a PCR buffer solution, Mg<2+>, dNTPs (deoxy-ribonucleoside triphosphates), four pairs of bacterial specific primers and four kinds of corresponding fluorescent probes. According to the detection kit and the application method thereof, four kinds of chicken-carried main food-borne pathogenic bacteria, namely Clostridium perfringens, Salmonella enteritidis, Escherichia coli O157: H7 and Campylobacter jejuni, can be rapidly and accurately detected by using quadruple fluorescent PCR, and the detection kit can be applied to the assay of bacteria, the diagnosis of diseases and epidemiological investigation.

An immunogenic composition, and method of using the composition, composed of a capsule polysaccharide polymer from one or more strains Campylobacter jejuni. The composition is either used alone or is conjugated to a carrier molecule, such as CRM₁₉₇. An aspect of the invention is that the immunogenic composition induces an immune response without the induction of Gulliam Barre Syndrome.

Methods using in vitro processes are disclosed for inducing or enhancing expression of enteric bacterial antigens or virulence factors. The methods, therefore, produce antigenically enhanced enteric bacteria. Also methods for using the antigenically enhanced bacteria are also disclosed, as well as vaccines containing the enteric bacteria. Specifically a whole enteric bacterium or components thereof are provided by Helicobacter species. Also there are other enteric bacteria which are useful for the disclosed invention; such as Campylobacter jejuni.

Immunogenic capsule polysaccharide polymer composition, and its method of producing, with improved structural integrity and immunogenic properties. The invention also relates to a method of using the compositions to elicit an immune response to Campylobacter jejuni.

The invention provides a method for detecting fluorescent quantitative PCR of 12 pathogenic bacteria in real time at the same time, including the steps: collecting specific pathogenic gene or toxin gene of the target pathogen and using it as a target gene to design primers and probes so as to make the reaction conditions consistent; extracting a genome template of a sample to be detected; adding the template respectively into tubules equipped with different specific upstream and downstream primers and probes, and then adding the corresponding fluorescent quantitative PCR reagents; under the same cycle of fluorescent quantitative PCR, the corresponding primers and probes are used to detect the samples simultaneously, quickly and quantitatively in their respective reaction tubes. Easier, Quick and efficient, Twelve common pathogenic bacteria (Escherichia coli O157: H7, Listeria monocytogenes, Salmonella, Vibrio parahaemolyticus, Streptococcus betae, Yersinia enterocolitica, Streptococcusfaecalis, Shigella, Proteus mirabilis, Vibrio fluvialis, Campylobacter jejuni, Staphylococcus aureus) can be detected simultaneously in drinking water and food economically.

The invention relates to a composite gene chip for detecting food pathogenic microbes, which comprises eight parallel sub-arrays constructed by detection probe groups for detecting each microbe in the eight food pathogenic microbes, blank lines are respectively arranged among the eight sub-arrays to be as intervals, thus constructing a big array; the eight food pathogenic microbes are listeria monocytogenes, comma bacillus, vibrio parahaemolyticus, salmonella, shigella, staphylococcus aureus, campylobacter jejuni and escherichia coli. The gene chip can clearly and accurately detect the species of eight target food pathogenic microbes, and also can definitely exclude other off-target microbe samples out of the eight target food pathogenic microbes. Each food pathogenic microbe detection sub-array on the chip can clearly and definitely distinguish off-target microbe samples from target microbe samples.

The inventive subject matter relates to a construct comprising antigens derived from multiple enterobacteria including Campylobacter jejuni capsule polysaccharide polymer, enterotoxigenic Escherichia coli recombinant polypeptide construct and lipopolysaccharide from Shigella spp. The subject invention also relates to a method of inducing an immune response utilizing the inventive composition.

The invention discloses a specific antibody of a campylobacter jejuni specific multi-epitope artificial polypeptide and coated immunomagnetic beads and an application thereof. The amino acid sequence of the artificial polypeptide prepared after tandem expression of a campylobacter jejuni specific surface protein multi-antigen epitope is SEQ ID No.1. A nucleotide sequence for encoding the campylobacter jejuni specific surface protein multi-antigen epitope is SEQ ID No.2. A kit for enriching campylobacter jejuni comprises immunomagnetic beads containing a specific antibody coated with the campylobacter jejuni specific multi-epitope artificial polypeptide. By applying the immunomagnetic beads or the kit, campylobacter jejuni can be enriched from a sample pre-enrichment liquid, and the sensitivity and detectable rate of subsequent separate culturing or PCR (Polymerase Chain Reaction) detection are increased greatly; and the specific antibody has the advantages of high specificity, high sensitivity, easiness, convenience, rapidness and suitability for large-scale sample detection.

The invention provides real-time fluorescence quantitative PCR (Polymerase Chain Reaction) primers and probe for detecting campylobacter jejuni and a method for carrying out quantitative detection by using the primers and probe. The nucleotide sequences of the primers and probe are respectively shown in SEQ ID NO.2, SEQ ID NO.3 and SEQ ID NO.4. The invention also provides a kit for detecting campylobacter jejuni. The detection method and the kit thereof provided by the invention have the advantages of accurate detection, high sensitivity, strong specificity, simplicity, convenience, quicknessand good clinical specimen detection capability.

The invention relates to a primer combination for detecting infectious diarrhea pathogen. The primer combination comprises at least one combination of the following combinations: a rotavirus primer combination, an enteric adenovirus primer combination, a Norovirus primer combination, a salmonella primer combination, a Shigella primer combination and a campylobacter jejuni primer combination. The invention also relates to a kit containing the primer combination. The kit also comprises a primer-coating micro-fluidic chip, an isothermal amplification reaction liquid, an isothermal amplification enzyme solution, and a negative control. The invention also relates to a detection method by using the above primer combination. The detection method comprises steps as follows: coating of the primer combination, nucleic acid extraction of a sample to be detected, LAMP reaction and result interpretation. The kit provided by the invention can detect infectious diarrhea pathogen rapidly and accurately within one hour, and is also of great significance for rapid assisted guide treatment and drug use. The multi-index detection also can be used in regional epidemiological investigation and epidemic surveillance so as to study epidemic situation of infectious diarrhea in China.

The invention relates to a campylobacter jejuni chitosan nanometer DNA vaccine, a preparation method and application thereof. The campylobacter jejuni chitosan nanometer DNA vaccine is a nano-microcapsule composed of a chitosan and a recombinant plasmid; the recombinant plasmid is a pCAGGS-flaA which consists of a campylobacter jejuni flagellin gene flaA and a eukaryotic expression vector; the flagellin gene flaA has a nucleotide sequence showed by an SEQ NO.1. The campylobacter jejuni chitosan nanometer DNA vaccine is made by dissolving the constructed recombinant plasmid in chitosan solution; the vaccine can also be used for preparing the drugs for preventing chicken campylobacter jejuni. Tests prove that the vaccine can stimulate to produce specific humoral and local mucosal immune response; after oral challenge, the cloaca bacteria exhausting rate shows a significant declining trend, and the number of the campylobacter jejuni in blood, small intestine, large intestine and caecum can be decreased.

The invention provides a high-sensitivity rapid molecule detection kit for campylobacter jejuni being one of food-borne pathogens; and the high-sensitivity rapid molecule detection kit comprises four high-specificity primers (two internal primers flhA-FIP/flhA-BIP and two external primers flhA-F3/flhA-B3) and a high-efficiency reaction system, wherein the four high-specificity primers aim at the gene flhA of a specificity detection target and can be applied to a loop-mediated isothermal amplification target sequence, and the high-efficiency reaction system can carry out the loop-mediated isothermal amplification target sequence. Simultaneously, the invention also discloses a method for detecting the campylobacter jejuni by using the kit. Compared with the detection sensitivity of a PCR (Polymerase Chain Reaction) detection method, the detection sensitivity of the kit and method provided by the invention for detecting the campylobacter jejuni is increased by more than 100 times, and the kit and method have high specificity on the detection of the campylobacter jejuni. The kit and the detection method provided by the invention have the advantages of high sensitivity, strong specificity, simple and convenient operation, short time consumption and low cost, thereby a high-efficiency reliable convenient and practical means is provided for detecting the campylobacter jejuni in food.

The inventive method and associated reagents relate to a molecular approach to determining Campylobacter jejuni capsule/Penner types. The invention also relates to a method of identifying Campylobacter jejuni types using primers in a multiplex PCR assay.

The invention discloses a detection kit of 16 food born pathogenic bacteria. The detection kit comprises a PCR amplification reagent and reference reagents; the PCR amplification reagent is composed of PCR buffer solution A, enzyme system B, and a primer probe mixed solution of the 16 food-borne pathogenic bacteria; the reference reagents are mainly composed of a negative control and a positive control; the PCR buffer solution A contains 5*Buffer, 25mM dN(U)TPs; the enzyme system B is mainly composed of HotStart Hi Taq DNA polymerase and UNG anti-pollution enzyme; the primer probe mixed solution of the 16 food-borne pathogenic bacteria contains a mixed solution of detection primer probes of salmonella, Shigella, enteropathogenic Escherichia coli, enterotoxigenic E. coli, enteroinvasive escherichia coli, adherent escherichia coli, Escherichia coli O157, Staphylococcus aureus, listeria monocytogenes, Bacillus Cereus, campylobacter jejuni, vibrio parahaemolyticus, Vibrio cholerae group O1, Vibrio cholerae group O139, Yersinia enterocolitica, and enterobacter sakazakii. The detection kit is high in detection sensitivity, is simple, and is convenient.

The invention relates to a Listeria monocytogenes monoclonal antibody hybridoma cell strain and an application thereof and belongs to the technical field of food safety immunological detection. According to the invention, a complete antigen of a specific polypeptide of Listeria monocytogenes is uniformly mixed with an equal volume of Freund adjuvant (Sigma), BALB/c mice are immunized by subcutaneous injection, after the BALB/c mice are immunized for four times, spleen cells of immunized mice are fused with mice myeloma cells of immunized mice by virtue of a PEG method, an indirect ELISA screening and three times of subcloning are carried out to obtain a monoclonal cell strain A. The monoclonal antibody secreted by virtue of the monoclonal cell strain A has cross reaction with different Listeria monocytogenes culture supernatants and no cross reaction with listeria except Listeria monocytogenes, E. coliO 157, Salmonella and Campylobacter jejuni and thus the Listeria monocytogenes monoclonal antibody hybridoma cell strain can be used in the specific detection of Listeria monocytogenes in a food.

The invention belongs to the technical field of biological detection and in particular relates to colloidal gold for campylobacter jejuni antigen detection, a glass fiber sample pad containing the colloidal gold, a detection reagent strip and a detection kit. The colloidal gold is prepared by virtue of a sodium citrate reduction method, and a polyvinylpyrrolidone stabilizer is added in the colloidal gold preparation process. The glass fiber sample pad comprises a colloidal gold-antibody conjugate and glass fibers; the campylobacter jejuni antigen detection reagent strip comprises a supporting plate, an antibody-nitrocellulose membrane, an absorbent pad, the glass fiber sample pad and a glass fiber sample adding pad; and the campylobacter jejuni antigen detection kit comprises a detection card and a detection strip. The colloidal gold for campylobacter jejuni antigen detection provided by the invention is uniform in particle size and high in dispersity and stability; and the campylobacter jejuni antigen detection reagent strip and the detection kit are fast, uniform and stable in color developing, high in sensitivity, convenient to use and easy to operate, and an organic unification of a complicated principle and simple operation is really realized.

The invention relates to a microaerophilic separation culture method of Campylobacter jejuni, which adopts the combination of a general candle-cylinder method and a pyrogallic acid method to create a microaerophilic environment. A directive streak cultivation method is adopted against the separation culture of the Campylobacter jejuni, a small amount of animal tissue detection samples are picked and directly inoculated on the selected culture medium by using connection annulus, the concentration of oxygen in the air is reduced by the candle-cylinder method with the combination of pyrogallic acid and anhydrous sodium carbonate mixture, an alkaline environment is formed by using anhydrous sodium carbonate so as to lead the pyrogallic acid to react with oxygen for absorbing the oxygen, therefore the culture environment meets the microaerophilic conditions required by the Campylobacter jejuni. Typical colonies obtained from separation culture are picked for PCR identification so as to further determine the Campylobacter jejuni. The microaerophilic separation culture method of Campylobacter jejuni has the advantages of low cost, simple operation, high detectable rate, good culture efficiency and the like, thus improving the separation culture efficiency of the Campylobacter jejuni and resolving the difficulty for cultivating the Campylobacter jejuni under microaerophilic condition.

The invention which relates to a Campylobacter jejuni detection method with a liquid chip belongs to the technical fields of immunization and food health detection. The invention which aims to provide a liquid chip utilization technology provides a Campylobacter jejuni detection method to realize the rapid Campylobacter jejuni detection. The liquid chip which is prepared in the invention is applied to detect Campylobacter jejuni. Compared with several other detection methods, the Campylobacter jejuni detection method with the liquid chip of the invention has the advantages of bidirectional high throughout of the detection sample number and the detection project (a large number of samples can simultaneously undergo detection projects with the number of high to ninety six), good flexibility, high sensitivity, good signal/noise ratio, simple operation, wide standard curve range, wide application range and the like. The liquid chip technology has a wide application prospect in fields of clinical diagnosis, infectious disease diagnosis by veterinarians, food microbe detection and the like when the technology is used as an advanced operation platform for the bioinformatics. The method which allows the rapid Campylobacter jejuni detection to be realized makes a contribution to the food safety.

A method for the detection and multiple, simultaneous quantification of any combination of Listeria sp., Staphylococcus aureus, Campylobacter jejuni and/or Escherichia coli O157.-H7, in one or more test samples, by multiplex amplification reaction, using real-time polymerase chain reaction (PCR). The steps in the method are: (a) extracting DNA from the test sample or samples; (b) preparing a reaction mixture specific for the pathogens to be detected and quantified, such that the reaction mixture contains the necessary reagents for enzymatic amplification of the extracted DNA and identification of the pathogens to be detected and quantified; (c) amplifying the reaction mixture by means of multiplex amplification reaction using PCR; and (d) simultaneously determining the presence or absence of and quantification of the pathogens in the test sample or samples. The method has the special feature that (i) the reaction mixture for enzymatic amplification of the DNA has sets of pairs of oligonucleotide primers identified as SEQ ID No. 1 and SEQ ID No. 2, SEQ ID No. 4 and SEQ ID No. 5, SEQ ID No. 7 and SEQ ID No. 8, and SEQ ID No. 10 and SEQ ID No. 11, and probes with oligonucleotide sequences identified as SEQ ID No. 3, SEQ ID No. 6, SEQ ID No. 9 and SEQ ID No. 12; (ii) the presence or absence of and quantification of said pathogens in any combination is determined by means of a fluorescent signal or fluorescence emission specific for each pathogen.

The invention relates to the technology of biological detection, and aims to provide a campylobacter jejuni rapid quantitative detection kit. The kit comprises a plurality of freezing tubes, wherein the freezing tubes A are filled with freeze-dried powder, the freeze-dried powder comprises the following ingredients: a probe-method fluorescent quantitative PCR pre-mixed liquid reagent as well as two primers and a probe which are used for fluorescent quantitative detection; the five freezing tubes B are respectively filled with sterile double distilled water; the five freezing tubes C are respectively filled with TZ lysate; the total number of the freezing tubes D, E, F, G, H, I and J is seven, the freezing tubes D, E, F, G, H, I and J are respectively filled with campylobacter jejuni standard substance DNA freeze-dried powder with different concentrations, and the concentration of the campylobacter jejuni is decreased progressively by ten times. The campylobacter jejuni rapid quantitative detection kit is realized on the basis of design of primer probes on campylobacter jejuni mapA genes and optimization of detection conditions. By a campylobacter jejuni rapid quantitative detection method, the number of campylobacter jejuni in a quantitative sample can be detected within a short time, and the minimum lower limit of detection can reach 102 CFU/mL; the campylobacter jejuni rapid quantitative detection method has good specificity, sensitivity and accuracy, is convenient and speedy, and is easy to operate.