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Lactobacillus reuteri lyophilized preparation and method for preparing the same

A technology of Lactobacillus and freeze-dried preparations, applied in the field of microbial feed additives and its preparation, can solve the problems of endangering human safety, food agriculture and animal husbandry, drug residues, and destruction of normal intestinal flora of animals, etc.

Inactive Publication Date: 2011-02-09
长沙伟嘉饲料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The problem in the breeding industry in recent years is the long-term use of antibiotics, which often destroys the normal flora and drug residues in the intestinal tract of animals, which poses a potential threat to humans. These problems have also endangered human safety and food safety. and the development of agriculture and animal husbandry
This strain has been included in the list of safe feed-grade microbial strains by (FDA) and (AAFCO). my country's Ministry of Health Announcement No. 3 in 2003 announced that Lactobacillus reuteri can be used as a microbial strain for human health food, but Lactobacillus reuteri was not included in the 5 types of Lactobacillus strains that can be added to feed published in 2006

Method used

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  • Lactobacillus reuteri lyophilized preparation and method for preparing the same
  • Lactobacillus reuteri lyophilized preparation and method for preparing the same
  • Lactobacillus reuteri lyophilized preparation and method for preparing the same

Examples

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Effect test

Embodiment 1

[0024] Example 1 Lactobacillus reuteri freeze-dried preparation

[0025] 1-1 Isolation and Identification of Lactobacillus

[0026] Dissect the live chicken, scrape 1g of the cecal mucosa by aseptic operation, put it in a triangular flask with glass beads filled with 9ml of sterile normal saline, shake it well, and then absorb 0.5ml of bacterial solution into the 4.5ml In a test tube of sterile saline, this dilution is 10 -1 , repeat the above process, and make 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 of bacteria liquid. choose 10 -4 、10 -5 、10 -6 For three dilutions, absorb 0.1ml of the bacterial solution and drop it on the MRS medium plate respectively, and cultivate it at 37°C for 24-48 hours by candle jar method, pick colonies with different shapes and inoculate them on the MRS medium for pure culture, and then place Store in a refrigerator at 4°C for later use.

[0027] Then pick the pure culture for staining and microscopic examination; select non-spore-forming Gram-...

Embodiment 2

[0074] Application effect of embodiment 2 Lactobacillus reuteri freeze-dried preparation in broiler chicken production

[0075] 1. Add the freeze-dried preparation to the feed of AA broilers according to different addition ratios. The test is divided into a blank group, an antibiotic group and a 0.3% freeze-dried preparation group, and the average Daily weight gain. At the end of 21 days and 42 days, compared with the blank group and the antibiotic group, the addition of Lactobacillus reuteri freeze-dried preparation group can increase the average daily gain of broilers. Table 4, Figure 5 .

[0076] Table 4 The effect of adding the preparation group on the daily gain of broilers

[0077] group

0～3W

4～6W

Full period (0~6W)

blank group

31.14±0.45

77.85±0.14

55.04±0.70

antibiotic group

31.92±0.01

79.55±0.08

56.29±0.45

Bacteria

32.49±0.41

82.77±0.12

58.22±0.61

...

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Abstract

The invention relates to a lactobacillus reuteri lyophilized preparation and a preparing method thereof, belonging to a microbial feedstuff preparation additive; the lactobacillus reuteri lyophilized preparation is characterized in that the weight percentage of the protectant of the lyophilized preparation is that 1 percent of sucrose, 12 percent of skimmed milk, 4 percent of lactose, 1 percent of glycerin, 2 percent of starch and the rest of water; the lactobacillus reuteri lyophilized preparation of the invention is separated by the mucous membrane of cecum of soy sauce chicken, and the lactobacillus reuteri is identified by Chinese industrial microbial culture collection management center; the bacterial strain of the invention is fermented and enlarged, and then is frozen and dried; the viable count of the freezing additive reaches 10<10>cfu / g, and can be used for feeding animals directly; the experiment of the safety of animals and the experiment of feeding the animals prove that the bacterial strain has higher safety and functionality.

Description

technical field [0001] The invention relates to a microbial feed additive and a preparation method thereof, in particular to a freeze-dried preparation containing live bacteria composed of Lactobacillus reuteri for direct feeding and a preparation method thereof. Background technique [0002] The problem in the breeding industry in recent years is the long-term use of antibiotics, which often destroys the normal flora and drug residues in the intestinal tract of animals, which poses a potential threat to humans. These problems have also endangered human safety and food safety. and the development of agriculture and animal husbandry. However, microbial preparations represented by lactic acid bacteria can play the opposite role of antibiotics. It can support the growth and reproduction of beneficial bacteria in the intestinal tract of animals, thereby inhibiting the growth of harmful bacteria, and finally achieve the role of maintaining the intestinal microecological balance. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12R1/225C12N1/20A23K1/18A23K1/16A23K10/18A23K50/75
Inventor 张董燕季海峰王四新黄建国单达聪王雅民
Owner 长沙伟嘉饲料有限公司
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