1321 results about "Lactic acid" patented technology

This invention provides an in vitro cell-free protein synthesis system, its reagent kit, and its applications, belonging to the field of protein synthesis technology. This invention improves an in vitro cell-free protein synthesis system based on Kluyveromyces lactis by adding exogenous L-arabinose, significantly enhancing the system's protein synthesis capacity. Furthermore, it provides a more efficient and higher-throughput in vitro protein synthesis reagent kit and a method for synthesizing exogenous proteins. The improvements provided by this invention can be achieved without molecular modification, are simple and convenient to operate, and save costs.

The invention belongs to the field of microorganisms. The invention provides a compound strain, a microbial inoculum and application of the compound strain in preparation of fermented East purple perilla tea. The compound strain comprises phytobacterium plantarum 2HDS3-La5 with the preservation number of CGMCC NO.34339, pediococcus acidilactici 2HDS3-SM6 with the preservation number of CGMCC NO.34337, Witzmania clostridium N2-La1 with the preservation number of CGMCC NO.34338, and the microbial inoculum is applied to preparation of fermented East purple perilla tea. The invention also provides application of the compound strain or a microbial inoculum containing the compound strain in preparation of fermented East purple perilla tea. The compound strain disclosed by the invention is applied to preparation of the fermented Dongbao tea, the content of substances with anti-inflammatory, anti-oxidation and immunoregulation effects in the Dongbao tea is remarkably increased by multiple times, and the prepared fermented Dongbao tea can remarkably improve the cell activity.

The invention provides a clostridium endophyllum engineering bacterium as well as a construction method and application thereof, and belongs to the technical field of biology, compared with a wild type strain, the clostridium endophyllum engineering bacterium can only produce acetic acid in a low-pH environment under the condition of taking CO2 and H2 as raw materials, and does not generate by-products such as ethanol, lactic acid, 2, 3-butanediol and the like. Besides, the fermentation liquor of the clostridium endophyllum engineering bacteria can be directly used as an acidic substrate to adjust the pH value of downstream yeast fermentation liquor, meanwhile, automatic addition of a carbon source is achieved, zero addition of inorganic salt in the cascade conversion process from CO2 and H2 to grease or yeast protein is achieved, and the economical efficiency and environmental friendliness of the whole process are improved.

The invention discloses bifidobacterium longum subsp.infantis capable of promoting bone growth and a metagen thereof, and belongs to the technical field of biology. The bifidobacterium longum subsp. Infantis CCFM1445 provided by the invention can promote the increase of bone volume fraction, femoral bone trabecula thickness and far-end growth plate thickness of mammals in a growth period, reduce the levels of serum bone resorption markers I-type collagen amino-terminal peptide and tartrate-resistant acid phosphatase, improve the levels of serum bone alkaline phosphatase and parathyroid hormone, and improve the clinical application value of the bifidobacterium longum subsp. Infantis CCFM1445. And the relative abundance of the other bacteria in the intestinal flora and the levels of creatine and indole-3-lactic acid are increased. The thermally inactivated bifidobacterium longum subsp. Infantis CCFM1445 can promote the increase of the volume fraction of femoral bones and the number of bone trabecula of malnourished mice and improve the N-terminal propeptide level of serum I-type procollagen. Therefore, the medicine or health care product prepared from the bifidobacterium longum subsp. Infantis CCFM1445 can be used for assisting in regulating bone metabolism and bone mass, and has a wide market prospect.

The invention provides application of lactic acid modified histone H3 in preparation of drugs and / or diagnostic kits for preventing and / or treating pancreatic ductal adenocarcinoma, and belongs to the technical field of disease diagnosis and / or treatment. The invention provides application of a lactic acid modified histone H3 as a target spot in preparation of a pancreatic ductal adenocarcinoma diagnostic kit or a medicine for preventing and / or treating pancreatic ductal adenocarcinoma. The site of lactic acid modification is 23-site lysine. The expression of H3K23la detected in clinical sample tissues in pancreatic ductal adenocarcinoma tumor tissues is obviously higher than that in para-carcinoma normal tissues, overexpression and non-expression of H3K23la in cells are regulated and controlled through in-vitro experiments in combination with overexpression and knock-down technologies, and results show that proliferation, invasion and migration capacities of pancreatic ductal adenocarcinoma cells are remarkably inhibited, so that the pancreatic ductal adenocarcinoma cells are remarkably inhibited. Therefore, the H3K23la can be used as a target spot for diagnosis and treatment of the pancreatic duct adenocarcinoma, and has wide application in diagnosis or treatment of the pancreatic duct adenocarcinoma.

The invention belongs to the technical field of biological medicine, and discloses lactococcus lactis HX21473 and application thereof, the lactococcus lactis HX21473 is preserved in China General Microbiological Culture Collection Center (CGMCC) on January 20, 2025, and the preservation number is CGMCC NO.33476. The invention further discloses a preparation method of the lactococcus lactis HX21473. The invention provides a strain of lactococcus lactis HX21473 capable of efficiently expressing a foreign protein. The lactococcus lactis HX21473 has strong acid resistance, can be used as an oral vaccine live carrier, and promotes research, development and application of oral vaccines.

The invention provides application of indole-3-lactic acid in preparation of uric acid reducing products, and relates to the technical field of medicines. The indole-3-lactic acid is found to have a good blood uric acid reducing effect for the first time, a new uric acid reducing medicine component is provided for health-care food, hyperuricemia and related diseases thereof, and the indole-3-lactic acid is small in renal function damage and is a safe and efficient uric acid reducing functional component. The gout treatment mechanism of the indole-3-lactic acid is mainly realized by relieving the inflammatory response of the organism and improving uric acid excretion, and the indole-3-lactic acid has dual effects, is different from the mechanism of the existing medicine, and has unique innovation.

The invention discloses a preparation method of high-viscosity cationic starch, which belongs to the technical field of modified starch, and comprises the following steps: crosslinking epichlorohydrin, ball-milling and cationizing; the epoxy chloropropane crosslinking comprises the following steps: mixing starch, calcium hydroxide and deionized water, stirring at 40-50 DEG C, adding sodium stearoyl lactylate, stirring at 50-55 DEG C, naturally cooling to room temperature, adding sodium chloride and epoxy chloropropane, stirring, dropwise adding a potassium hydroxide aqueous solution, stirring, adjusting the pH value to 6, filtering, taking filter residues, washing and drying to obtain crosslinked starch; in the step of ball milling, calcium hydroxide, sucrose fatty acid ester SE-11 and deionized water are mixed and then subjected to ball milling, and a ball-milled material is obtained; the prepared high-viscosity cationic starch is high in paste liquid viscosity and cationic substitution degree, good in paste liquid viscosity stability, uniform in substitution degree and paste liquid viscosity, high in reaction efficiency in cationization and low in alkali consumption.

Disclosed is a strain of Bifidobacterium longum subsp. longum named dipro-O. The Bifidobacterium longum subsp. longum dipro-O used in the present invention has the ability to overproduce BSH, the ability to reduce cholesterol, and the ability to metabolize tryptophan into indole-3-lactic acid, can effectively reduce body weight, significantly lower insulin resistance levels resulting from high-fat diets, reduce the expression of the inflammatory cytokine TNF-α, significantly lower the levels of total cholesterol (TC) and low-density lipoprotein (LDL) in the blood, and increase the level of high-density lipoprotein (HDL), and can ameliorate disorders of the gut microbiome structure. In addition, the strain can also have an ameliorating effect on other lipid metabolism-related diseases such as hyperlipidemia, thereby providing health interventions for the host's body weight, liver, intestinal health, and blood lipid levels. Moreover, pasteurized dipro-O can also have the effect of regulating lipid metabolism, further extending the range of applications of the strain.

The invention relates to the technical field of intelligent fermentation, in particular to a control system of a yogurt fermentation device, which comprises an acquisition module, a trigger generation module, a judgment module, an identification module, a rate adjustment module, a correction module and a threshold adjustment module. According to the method, key parameters in the tank are collected in real time, initial risks such as high temperature or acidity abnormity in the tank are found in time, the abnormity type is judged according to the lactic acid generation rate and the milk curd viscosity change, and therefore the abnormity index is calculated to adjust the stirring rate, the adjustment result is corrected according to the whey separation rate, and the accuracy of the adjustment result is improved. The temperature threshold is automatically adjusted according to the fluctuation condition of the corrected stirring rate within a certain time, so that dynamic balance is formed among the temperature, the pH value, the lactic acid generation rate and the curd viscosity; the problem that fermentation abnormity is difficult to recognize and adjust in time due to single parameter monitoring and empirical threshold judgment is effectively solved.

The invention belongs to the technical field of new energy materials, and particularly relates to a preparation method of a bamboo-based hard carbon sodium ion battery negative electrode material, which specifically comprises the following steps: (1) slicing, crushing, screening, sieving, drying and treating fresh bamboo under steam pressure to obtain bamboo powder; (2) bamboo powder and lactococcus lactis liquid are mixed and fermented, and after fermentation is completed, the bamboo powder and the lactococcus lactis liquid are washed to be neutral and dried; (3) placing the bamboo powder subjected to fermentation treatment in an inert atmosphere, heating, keeping constant temperature, and cooling to obtain a pre-carbonized sample; (4) soaking the pre-carbonized sample in a hydrochloric acid solution, continuously reacting, washing to be neutral, and drying; and (5) continuously heating the acid-washed and dried material in the step (4) in the inert atmosphere, carbonizing at a constant temperature, and cooling to obtain the final sodium-ion battery negative electrode material. The method disclosed by the invention has the advantages of remarkable environmental friendliness, low cost, simplicity and convenience in operation and the like; and the battery negative electrode material prepared by the method disclosed by the invention has excellent electrochemical performance.

The invention discloses pediococcus acidilactici TH903 and application thereof in directional preparation of rare ginsenoside Rg4 by fermenting ginseng berries, and belongs to the field of fermentation engineering, the pediococcus acidilactici TH903 is preserved in the China Center for Type Culture Collection on September 29, 2025, the preservation number is CCTCC NO: M20252155, and the preservation unit is the China Center for Type Culture Collection. The Pediococcus acidilactici TH903 is utilized to successfully and directionally convert ginsenoside Re in the ginseng berry extract into rare ginsenoside Rg4, the rare ginsenoside Rg4 is mainly used as a fermentation product, the main target product, namely the rare ginsenoside Rg4, is accurately prepared, and the Pediococcus acidilactici TH903 has relatively high specificity, and can be used for preparing the rare ginsenoside Rg4. Meanwhile, the method has the advantages of high conversion efficiency and high conversion product content, and a cheap and efficient preparation process is established for the rare ginsenoside Rg4.

The invention discloses a preparation process of a rare earth precipitation resistant electrophoretic coating for an automobile body. The preparation process comprises the following steps: S1, preparing a stable solution; s2, preparing a rare earth chelating solution; adding lanthanum lactate into the stable solution for multiple times, and adding ammonium ceric sulfate for multiple times after the lanthanum lactate is completely dissolved; s3, preparing a resin base solution; s4, preparing mixed slurry; s5, adding a solvent and an auxiliary agent; a five-membered ring chelate with thermodynamic stability is formed by carboxyl, hydroxyl and rare earth elements in sodium citrate, rare earth ions can be tightly wrapped through strong coordination bonds, namely, empty orbits of the rare earth ions and lone pair electrons of a chelating agent form coordination bonds, so that the rare earth ions cannot be dissociated in a system, and the rare earth ions are prevented from being dissociated in the system. And a path of generating hydroxide precipitates by free rare earth ions is thoroughly blocked, so that rare earth elements are effectively ensured to be uniformly distributed in the electrophoretic paint, and the surface of a raw material treated by a subsequent electrophoresis process is more uniform.

Escherichia coli is modified by means of metabolic engineering, a genetically engineered bacterium for high yield of O-succinyl-L-homoserine is obtained, and a construction method of the genetically engineered bacterium comprises the following steps: weakening a synthetic route of an organic acid metabolic byproduct of a chassis strain E.coli W3110 [delta] metI [delta] metJ [delta] thrB [delta] metB; wherein the synthetic route of the organic acid metabolic byproducts is selected from at least one of a formic acid synthetic route, a lactic acid synthetic route and an ethanol synthetic route. The constructed genetically engineered bacterium is applied to microbial fermentation production of the O-succinyl-L-homoserine, effective accumulation of the O-succinyl-L-homoserine is achieved, the sugar acid conversion rate reaches 46.8%, and a foundation is laid for subsequent construction of high-yield O-succinyl-L-homoserine engineering bacteria.

The invention relates to lactobacillus gasseri FMTA1121E with an effect of regulating a vaginal micro-ecological environment and application of the lactobacillus gasseri FMTA1121E. The lactobacillus gasseri FMTA1121E is classified and named as lactobacillus gasseri, and the preservation number of the lactobacillus gasseri FMTA1121E is CGMCC No.34962. The lactobacillus gasseri FMTA1121E can be metabolized to generate beneficial components such as lactic acid, gamma-aminobutyric acid and hydrogen peroxide so as to regulate the genital tract environment and improve the health degree; and the bacillus subtilis has excellent bacteriostasis and sterilization effects on various common vaginal pathogenic bacteria such as escherichia coli, staphylococcus aureus, gardnerella vaginalis, candida albicans, neisseria gonorrhoeae and the like. Therefore, the lactobacillus gasseri FMTA1121E strain can be applied to preparation of preparations for preventing and treating related genital tract diseases such as vaginitis.

The present application relates to the technical field of traditional Chinese medicine, in particular to a kind of slimming traditional Chinese medicine composition and preparation method thereof.The present application uses the raw materials such as Buzha Leaf, hawthorn, lotus leaf, immature fruit of Chinese citron, magnolia officinalis, raphanus sativus, dried orange peel, pellitory, alisma orientalis and cassia obtusifolia, after decoction, lactic acid lactobacillus lactis CICC 6242 and royi mucous lactobacillus CICC 6121 are fermented with 0.4-0.8:1 ratio, then combined enzymolysis is carried out using ginger protease and snail protease, and then 15-25wt% ethanol is used for extraction, and the composition is endowed with excellent lipid-lowering and weight loss effect.

The invention discloses an extraction method of safflower leaf exosome-like nano-vesicles and application of the safflower leaf exosome-like nano-vesicles in preparation of drugs for preventing and treating adriamycin-induced cardiotoxicity, fresh safflower leaves are taken and cleaned, a PBS solution is added for crushing and filtering, filtrate is collected and continuously centrifuged, supernate is taken and subjected to ultracentrifugation, precipitates are collected after centrifugation, and the safflower leaf exosome-like nano-vesicles are obtained; and filtering and sterilizing by using a filter membrane to obtain the safflower leaf exosome-like nano-vesicles. The carthamus tinctorius leaf exosome-like nano-vesicles can significantly improve the heart function of doxorubicin-induced cardiotoxic mice; myocardial injury induced by doxorubicin can be repaired, and myocardial fibrosis induced by doxorubicin can be inhibited; the content of a myocardial injury marker lactic dehydrogenase in body serum can be reduced, a new treatment strategy is provided for preventing and treating adriamycin-induced cardiotoxic diseases, and the application prospect is good.

The invention relates to the field of comprehensive utilization of biomass resources and preparation of high polymer materials, in particular to a green preparation method of high-deacetylation-degree chitin capable of recycling reaction waste liquid. The invention aims to solve the problems of serious environmental pollution, resource waste, low product purity and incomplete waste liquid recycling in chitin extraction. The method comprises the following steps: by taking crustacean biomass as a raw material, firstly carrying out high-voltage pulse electric field pretreatment to crush the tissue structure of the raw material and improve the reaction activity, and then heating and extracting in a choline chloride-lactic acid deep eutectic solvent system to realize efficient removal of proteins and inorganic salts in the raw material; according to the method, the chitin and the alkali liquor are subjected to a rapid deacetylation reaction by adopting microwave assistance and cooperating with the action of the phase transfer catalyst; according to the method, a waste liquid graded recycling system is adopted, hydrochloric acid in waste acid liquid generated by decalcification is recycled through electrodialysis, alkali liquid is recycled through ultrafiltration-nanofiltration double-membrane combination, deep eutectic solvent components are recycled through reduced pressure distillation, and recycling of acid, alkali and a solvent is achieved.

A Bifidobacterium longum subsp. longum dipro-017. The Bifidobacterium longum subsp. longum dipro-017 used has the ability to metabolize tryptophan to produce indole-3-lactic acid, can inhibit the pathogenic bacteria Salmonella Typhimurium, Staphylococcus aureus, and Escherichia coli, and can effectively mitigate weight loss caused by ulcerative colitis, improve a DAI score, improve colon length, mitigate the content of lipopolysaccharide (LPS) in blood, and increase the level of anti-inflammatory cytokine IL-10, as well as mitigate the content of bone metabolism indicators tartrate-resistant acid phosphatase (TRAP) and N-terminal propeptide of type I procollagen (PINP) in blood.

The invention belongs to the field of food production and processing, and requests to protect a production process of compound lactobacillus fermented chilli sauce and a method for preparing sour soup, fresh red chilli is used as a main raw material, and the chilli sauce raw material is prepared by cleaning, selecting, crushing, preheating, grinding, pulping, adding or not adding edible glacial acetic acid or citric acid, concentrating, sterilizing and aseptically filling; performing blending and temporary storage, pre-sterilization, cooling, compound lactobacillus strain pre-dissolution, feeding, stirring, fermentation, re-sterilization and sterile filling to obtain the compound lactobacillus fermented chilli sauce, and blending the compound lactobacillus fermented chilli sauce with fermented tomato sauce, edible oil and the like to obtain the sour soup. Industrial compound lactic acid bacteria are adopted, various organic acids, amino acids and the like are generated through metabolism, the fermented chili sauce is endowed with unique sour and fragrant flavor, the taste is mellower, and the fermentation process is controllable and stable; meanwhile, the prepared fermented chilli sauce is innovatively applied to sour soup products, the standardization degree of the products is high, and the production efficiency is improved.

The application relates to a modified polylactic acid oligomer and a preparation method and application thereof. The modified polylactic acid oligomer has the structure shown in the following formula (I). The modified polylactic acid oligomer obtained by copolymerization of 5-hydroxy-2-hexenoic acid and lactic acid has more excellent and stable and persistent antibacterial activity, the antibacterial efficiency is greatly improved, the sterilization rate is improved, and the sterilization time is shortened; when the modified polylactic acid oligomer is applied to various antibacterial coatings and textile finishing, the surface no longer has a wet sticky feeling, the commercialization and wide application of the material are more beneficial, and the modified polylactic acid oligomer has higher affinity to a target substrate, so that the persistence, stability and washability of a surface coating can be improved.

The application belongs to the technical field of dechlorination carrier and specifically provides a preparation method of a dechlorination active carrier, which comprises the following steps: 1) polyoxyethylene-polyoxypropylene block copolymer and modified protein solution are dissolved in deionized water, then cobalt nitrate, aluminum lactate and 2-aldehyde benzene boronic acid are added and stirred uniformly, reaction is carried out at 110-130 DEG C, centrifugation and drying are carried out to obtain a precursor; 2) tetrabutylphosphonium hydroxide and sodium aluminate are added into deionized water, TEOS and the precursor are added after mixing, stirring is carried out, crystallization is carried out at 165-175 DEG C, centrifugation and washing are carried out, and the obtained solid is dried and calcined to obtain the dechlorination active carrier. The carrier prepared by the application has the advantage of good dechlorination effect.

The invention discloses combined probiotics for improving sarcopenia as well as a preparation method and application of the combined probiotics, and belongs to the field of probiotics. The invention relates to a combined probiotic for improving sarcopenia. The combined probiotic comprises a lactobacillus reuteri body, a lactobacillus johnsonii body, a lactobacillus fermentum body and a bifidobacterium adolescentis body. The combined probiotics disclosed by the invention can be used for remarkably improving the overall symptoms of sarcopenia model mice, including inhibiting weight loss, constipation symptoms and colonic mucosa destruction, prolonging load swimming time, improving muscle endurance, improving skeletal muscle atrophy, improving the expression of I-type slow muscle fiber protein MyHC I and promoting slow muscle fiber repair, and has the effects of preventing and treating sarcopenia. The invention discloses a probiotic intervention method for treating sarcopenia, which comprises the following steps of: preparing probiotics for treating sarcopenia, reducing the content of inflammatory cytokines in muscular tissues, increasing the content of hepatic glycogen, improving systematic metabolism of mice with sarcopenia and damaged serum markers, including lactic acid, lactic dehydrogenase, creatine kinase, creatinine, alanine transaminase ALT and aspartate transaminase AST, providing a new scheme for probiotic intervention for sarcopenia, and being used for preparing related functional products or intervention means.

The invention provides a composite antifouling system coating as well as a use method and application thereof. The coating sequentially comprises a bottom layer, a copper-containing middle layer and a surface layer in the direction from near to far away from a base material, wherein the copper-containing middle layer comprises 20-30 parts of self-polishing acrylic silicon resin, 5-10 parts of lime rosin, 8-12 parts of zinc oxide, 30-50 parts of cuprous oxide, 2-3 parts of a compound antifouling agent, 0.5-1 part of a thixotropic agent and a first diluent. The surface layer is prepared from 30 to 35 parts of caprolactone and lactic acid grafted linear polyester, 5 to 10 parts of highly hydrolyzed N-(hydroxymethyl) acrylamide (NMA) modified acrylic resin, 15 to 20 parts of copper-free organic antifouling agent, 5 to 12 parts of polytetrafluoroethylene micro powder, 2 to 3 parts of dispersing agent and second diluent. The high-hydrolysis copper-free surface layer forms an isolation barrier, so that the copper-containing middle layer is prevented from being in direct contact with air, and the problem that the antifouling efficiency is reduced due to the fact that water is not discharged for a long time after coating is solved; the stable hydrolysis rate of the surface layer resin can ensure the continuous release of the organic antifouling agent, and the copper-containing intermediate layer can provide deep antifouling guarantee and synergistically realize long-acting antifouling.

The invention discloses a preparation method of a fructus cannabis protein peptide product with effects of resisting fatigue and improving exercise ability, which comprises the following steps: performing preliminary crushing, ultralow-temperature freezing micro-crushing and low-temperature continuous phase-change extraction degreasing on fructus cannabis, performing step-by-step hydrolysis by using polysaccharase and 2.4 L bacterial protease to extract fructus cannabis protein, performing low-temperature salting-out, and performing freeze-drying to obtain the fructus cannabis protein peptide product with the effects of resisting fatigue and improving exercise ability. The fructus cannabis protein peptide powder is prepared through low-temperature ultrasonic dissolution promotion, high-pressure micro-jet homogenization, compound enzyme C hydrolysis, 1% pepsase, high-temperature enzyme deactivation, chlorophyll removal through counter-current extraction, hydrogen peroxide decoloration, ultralow-temperature instantaneous concentration, low-temperature vacuum belt type drying and low-temperature ultrahigh-pressure sterilization. The fructus cannabis protein peptide prepared by the invention has the following remarkable effects: 1, the exercise ability is remarkably improved, the accumulation of lactic acid and urea nitrogen is reduced, the exhaustion time is prolonged, and the physical and mental fatigue is relieved; 2, the content of hepatic glycogen and muscle glycogen is obviously increased, the energy reserve of the body is increased, the explosive power and the reactivity are improved, and the athletic performance is improved; and 3, the antioxidant level of the body is improved, and fatigue and immune recovery after exercise are promoted.

The invention discloses a cellulose microcrystal reinforced degradable flame-retardant PLA composite material and a preparation method thereof, and belongs to the field of bio-based functional polymer materials. The composite material is prepared from the following components in parts by weight: 80 to 100 parts of polylactic acid (PLA), 3 to 10 parts of bamboo cellulose microcrystal, 3 to 10 parts of biomass polyphosphate, 1 to 5 parts of GO-g-PLA, 10 to 30 parts of PBAT toughening agent and 0.2 to 1 part of auxiliary agent. The preparation method comprises the following steps: (1) preparing high-crystallinity bamboo cellulose microcrystals by adopting a supercritical CO2 method; (2) preparing a GO-g-PLA interface compatilizer through graft polymerization; (3) synthesizing a biomass polyphosphate flame retardant by taking pentaerythritol and diphenolic acid as raw materials; and (4) melting, blending and extruding the components. Through the synergistic effect of a flame retardant-GO-BCMC three-dimensional network, the material has excellent mechanical properties (the tensile strength is 45-47 MPa, and the impact strength is 10-12 kJ / m), high flame retardance (LOI is 28-29%, UL-94 V-0 level) and environmental friendliness (the 180-day biodegradation rate gt); the composite material can be widely applied to the fields of electronic and electric appliances, automotive trim, medical materials and the like.

The invention discloses a soil-improving growth-promoting modified lignin soil conditioner and a preparation method thereof, and belongs to the technical field of high polymer materials and soil improvement. The modified lignin soil conditioner comprises an agent A, an agent B and an agent C, the agent A is a cationic modified lignin polymer and is prepared by graft copolymerization of lignosulfonate and a cationic monomer in a free radical initiation mode; the agent B is lignin controlled-release particles and is prepared by wrapping an amino acid amphoteric regulator in the cationic modified lignin polymer and another negative charge natural polymer through electrostatic intercalation self-assembly; the agent C is at least one of citric acid, lactic acid, itaconic acid, fulvic acid, wood vinegar, maleic acid, maleic anhydride and polyacrylic acid. The soil conditioner disclosed by the invention has the advantages of low cost, environment friendliness and efficient repairing performance, and an innovative solution is provided for agricultural soil improvement.

The invention discloses a method for efficiently preparing high-activity hericium erinaceus polysaccharide from waste in the edible mushroom industry, and belongs to the technical field of deep processing of edible mushrooms and recycling of waste. The method comprises the following steps: carrying out steam explosion pretreatment on hericium erinaceus waste mushroom residues, and carrying out synergistic compatibility on the hericium erinaceus waste mushroom residues, tea residues, bean residues and citrus residues which are subjected to specific enzymolysis to prepare a liquid fermentation culture medium; a two-stage control strategy is adopted for submerged fermentation of hericium erinaceus liquid, and hypha proliferation and polysaccharide synthesis conditions are optimized; and after fermentation is finished, extracting and purifying mycelium polysaccharide by adopting a choline chloride-lactic acid eutectic solvent system. According to the method, high-valued utilization of the edible fungus chaff and various agricultural byproducts is achieved, the content of beta-(1-> 3)-D-glucan in the prepared hericium erinaceus polysaccharide is larger than or equal to 55%, the immunocompetence (IC50) reaches 32 micrograms / mL, the hericium erinaceus polysaccharide is remarkably superior to commercially available products, and the hericium erinaceus polysaccharide can be directly used as functional food and medicine raw materials. The process is green and efficient, and has remarkable economic and environmental benefits.

The invention belongs to the technical field of gene engineering and cell transformation, and discloses a gene engineering construction method and application of anti-apoptosis human mesenchymal stem cells. Through HDAC1 K412 site specific lactic acid mutation, breakthrough improvements are realized: for example, CRISPR-Cas9-mediated accurate gene editing is utilized, only a single amino acid site is changed, that is, K412R mutation simulates persistent lactic acid, and whole genome apparent modification disturbance can be avoided; the modification does not depend on an exogenous metabolic substrate, and the negative feedback effect of traditional metabolic intervention is overcome; the mutant continuously inhibits a P53 apoptosis pathway, so that the oxidative stress mediated apoptosis resistance of the MSC is remarkably improved. According to the design concept of one target spot and one regulation, the core contradiction that long-term effectiveness and specificity cannot be considered in the prior art is fundamentally solved, and a brand new normal form is provided for stem cell treatment.

The invention provides a method for preparing a coarse cereal fermented staple food based on an enzyme-bacterium synergistic effect, belongs to the technical field of coarse cereal staple food processing, and aims to solve the problems that the existing coarse cereal staple food is high in hardness, rough in taste, obvious in oral adhesion feeling, loose in structure and poor in flavor coordination in a cold state. The preparation method comprises the following steps: pretreating raw materials, preparing dough through cooperation of bacteria and enzymes, and fermenting and steaming, wherein the raw materials comprise lactic acid esterification modified inulin, enzyme microcapsules, coated enzymes, calcium carbonate-starch composite sustained-release microspheres and the like. The preparation method provided by the invention can effectively improve the cold-state comprehensive quality of the coarse cereal staple food, maintain low cold-state hardness, roughness and adhesive force, reduce the residue falling rate, ensure pure flavor and no beany flavor, stabilize the calcium dissolution rate, adapt to non-heating eating scenes such as daily room temperature and low temperature in winter, and realize the consideration of cold-state quality and nutrient enrichment.