61 results about "Jejunum" patented technology

The invention belongs to the technical field of microbial genetic engineering, and particularly relates to an alpha-2, 3-sialyltransferase mutant and application thereof. The a-2, 3-sialyltransferase mutants V175N, P198A, G173D, D174Q, F196K and A315H are obtained by performing site-specific mutagenesis on six amino acids in an a-2, 3-sialyltransferase amino acid sequence coded by a gene derived from Campylobacter jejuni (OH4384), the yield of 3 '-sialyllactose is remarkably increased by single-point mutagenesis of the V175N, the yield of 3'-sialyllactose is remarkably increased by single-point mutagenesis of the V175N, the yield of 3 '-sialyllactose is remarkably increased by single-point mutagenesis of the P198A, the G173D, the D174Q, the F196K and the A315H, the optimal recombinant microbial strain 3 '-SL-09 (V175N / P198A / D174Q) is obtained through combination of multiple mutation sites, the yield can reach 5.91 g / L, the bottleneck problem of low catalytic efficiency of sialyltransferase is effectively solved, and an enzyme element with excellent performance is provided for high-efficiency and low-cost biological manufacturing of 3'-SL.

The invention belongs to the technical field of biological medicines, and particularly relates to application of UH15-38 in preparation of a medicine for preventing and treating PEDV-PoRVA co-infection. According to the invention, a PEDV and PoRVA co-infected piglet model is systematically established for the first time, a high-throughput transcriptomics means is combined, an enhancement mechanism of co-infection on host pathogenicity is deeply analyzed from an immune regulation level, and based on the mechanism, UH15-38 is found to be capable of effectively treating PEDV and PoRVA co-infected piglets. Experimental data show that the UH15-38 remarkably relieves clinical diarrhea symptoms caused by co-infection, reduces the copy number of PoRVA and PEDV viruses, effectively inhibits duplication of the viruses in intestinal tracts, remarkably reduces the expression levels of IL-1beta and p-MLKL in jejunum tissues, and reduces necrotic apoptosis and inflammatory response. The UH15-38 can provide a new application value for the treatment of the porcine viral diarrhea.

The invention discloses a probiotic biofilm pulse ultrasonic atomization spraying device, and particularly relates to the technical field of jejunum nutrient canal processing.The device comprises a base, and a spraying sleeve shell and an electric frame are installed at the top of the base; an inner conversion frame is rotationally arranged in the spraying sleeve shell, a linear placement groove is formed in the outer side of the inner conversion frame, and multiple sets of vacuum fixing clamps are slidably connected to the inner walls of the two sides of the linear placement groove; a rotary drawing mechanism is arranged in the electrical frame and comprises a fixed feeding pipe, a drawing sleeve and an air supply shaft seat; the drawing sleeve penetrates through the middle of the air supply shaft seat and is in threaded connection with the air supply shaft seat, the air supply shaft seat is provided with a second cooling pipeline and an air outlet ring cavity from inside to outside, a first communicating hose is connected between the second cooling pipeline and the fixed feeding pipe, a vortex sheet is arranged in the air outlet ring cavity, and the air outlet ring cavity can be filled with inert gas; and piezoelectric ultrasonic atomization sheets are mounted in the end parts of the cooling pipeline II and the drawing sleeve. Environmental microbial pollution is effectively prevented, and the spraying effect of the probiotic biological membrane is guaranteed.

The invention discloses application of bifidobacterium longum subsp. Infantis CCFM1269 in preparation of a product for relieving lactoglobulin allergy, and belongs to the technical field of microorganisms. The bifidobacterium longum subsp. Infantis CCFM1269 provided by the invention can relieve food allergy, and the food allergy can be relieved specifically as follows: (1) allergy symptom score and diarrhea occurrence rate can be relieved, and anus temperature and immune organ index can be recovered; (2) the immune response is regulated, the levels of histamine, total IgE, IgG1, beta-lactoglobulin specific IgE and proinflammatory factors IL-4 and IL-17A in serum are reduced, the levels of IFN-gamma and IL-10 are improved, and the immune balance is recovered; (3) inflammation of lung and jejunum tissues is relieved; (4) improving the oxidative stress state; (5) the intestinal barrier function is enhanced; and (6) recovering the diversity of intestinal flora, and adjusting the relative abundance of key bacteria genus such as Alloprevotella and the like.

The utility model discloses a mould is placed under the mould of simulation ultrasonic real -time guidance of nasal -intestinal tube, including the digestive tract simulation body of simulation esophagus, stomach, duodenum and jejunum, it adopts the flexible material of ultrasonic wave penetration, front surface is not transparent, back surface is transparent, and its inside esophagus is used for filling air, and the part except esophagus is used for filling liquid, the digestive tract simulation body is fixed between two organic glass, front surface is not transparent, back surface is transparent, the organic glass of front surface opens at esophagus place, antrum gastricum place respectively, as the acoustic window of ultrasonic, is used for the inflation sign of esophagus and antrum gastricum nasal -intestinal tube catheter tip cloud sign of ultrasonic observation when placing nasal -intestinal tube respectively, is provided with many resistance positions at stomach and duodenum, for making nasal -intestinal tube catheter tip forward into the obstruction. The utility model can simulate the sign of ultrasonic under different conditions on the mould, effectively simulates the actual entering process of nasal -intestinal tube under the real -time guidance of ultrasonic, and facilitates simulation teaching demonstration.

This invention discloses a fermented microbial preparation for inhibiting Campylobacter jejuni in calves, its preparation method, and its application. The fermented microbial preparation is a co-fermentation product of a mixture of Lygodium japonicum decoction and liquid culture medium, inoculated with Lactobacillus paracasei; the Lactobacillus paracasei is... Lacticaseibacillus paracasei KQNIU. Comparative experiments in this invention revealed that, compared to other types of traditional Chinese medicine, the inhibitory effect of fermented Lygodium japonicum on Campylobacter jejuni is significantly higher than that of other types of traditional Chinese medicine. Furthermore, the co-fermentation of the traditional Chinese medicine Lygodium japonicum and the probiotic Lactobacillus paracasei KQNIU achieves a synergistic effect between the two, overcoming the problem of limited efficacy when used alone.

1. Name of the product in this design: Anastomosis device (inserted fully endocavitary electrically powered tubular type - straight). 2. Application of this design: for laparoscopic anastomosis of esophagus and jejunum, intestine to intestine, or thoracoscopic anastomosis of esophagus and jejunum or esophagus and stomach within the thoracic cavity. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D view 1. 5. Other situations requiring explanation: Part A is made of transparent material.

The application discloses application of Fagopyrum cymosum root powder in preparation of products for improving egg quality or intestinal health of laying ducks, and belongs to the technical field of feed additives. The application provides application of Fagopyrum cymosum root powder in preparation of products for improving egg quality or intestinal health of laying ducks, and the Fagopyrum cymosum root powder can improve eggshell strength, yolk color and eggshell proportion, improve the content of aspartic acid, threonine, glutamic acid, glycine, cystine and leucine in yolk, improve the content of arachidonic acid and arachidonic acid in yolk, and reduce the content of saturated fatty acid in yolk; the Fagopyrum cymosum root powder can improve the activity of maltase in the mucous membrane of the jejunum of laying ducks, and can improve the diversity and abundance of intestinal flora of laying ducks. The application provides a new strategy for improving egg quality or intestinal health of laying ducks.

The application discloses a compound plant essential oil preparation for improving intestinal tract barrier function of laying hens and a preparation method thereof, relates to the technical field of livestock and poultry intestinal tract health regulation, and comprises microcapsule particles with a multilayer structure, which sequentially comprises a core layer, a first coating layer, an intermediate functional layer and a second coating layer; the compound plant essential oil preparation for improving intestinal tract barrier function of laying hens and the preparation method thereof are characterized in that the preparation is arranged as a multilayer microcapsule structure composed of the core layer, the first coating layer, the intermediate functional layer and the second coating layer in sequence, and the first coating layer and the second coating layer adopt enteric polymers with different pH dissolution ranges, so that the intermediate functional layer can be released first in the pH environment from the duodenum to the jejunum, and the core layer is released subsequently in the pH environment of the ileum, thereby realizing the graded and zoned release of active components of plant essential oils in the front and rear sections of the small intestine of laying hens, and matching different essential oil components with the barrier repair requirements of each section of the intestinal tract.

The utility model discloses a multifunctional bedside nasojejunum nutrition tube placement training device which comprises a head and neck bionic block, a nasopharyngeal cavity channel is arranged in the head and neck bionic block; the pleuroperitoneal cavity frame comprises a hard simulation frame and bionic skin covering the hard simulation frame. Wherein the head and neck bionic block and the pleuroperitoneal cavity frame are integrally formed or detachably connected. A bionic esophagus, a bionic stomach, a bionic duodenum and a part of bionic jejunum which are sequentially connected are arranged in the pleuroperitoneal cavity frame, and a bionic cardia and a bionic pylorus are arranged at the head end and the tail end of the bionic stomach respectively. A bionic inferior vena cava, a bionic abdominal aorta, a bionic mesenteric superior vein and a bionic mesenteric superior artery are further arranged in the pleuroperitoneal cavity frame; the bionic inferior vena cava and the bionic abdominal aorta are arranged on the rear sides of the bionic stomach and the bionic duodenum, the bottom ends are closed, and the head ends penetrate out of the bionic skin; the bottom ends of the bionic superior mesenteric vein and the bionic superior mesenteric artery are closed, and the head ends of the bionic superior mesenteric vein and the bionic superior mesenteric artery partially wrap the bionic duodenum and then penetrate out of the bionic skin.

An antimicrobial peptide having an amino acid sequence selected from SEQ ID NOs: 2-12 is provided. The antimicrobial peptide has antimicrobial activity against Gram-negative bacteria, Campylobacter jejuni, Escherichia coli, Salmonella enterica subsp. enterica serovar typhimurium, Salmonella enterica subsp. enterica serovar choleraesuis, Klebsiella pneumonica, and / or Enterobacter cloacae. A composition, pharmaceutical composition, food additive, cosmetic composition, or hygine product having an antimicrobial peptide including an amino acid sequence selected from SEQ ID NOs: 2-12 is also provided. A method of treating an infectious disease caused by bacteria including a step of administering a pharmaceutical composition having the antimicrobial peptide as an active ingredient is also provided.

The application provides a jejunum catheterization intelligent navigation method and system based on real-time ultrasonic contrast, relates to the technical field of biological medical engineering, and comprises the following steps: collecting multiple data sets; performing unified time base alignment on the multiple data sets, and performing structure encapsulation and normalization processing to obtain jejunum catheterization navigation acquisition data frames; performing harmonic selective extraction on the contrast frames in the jejunum catheterization navigation acquisition data frames to obtain a harmonic selective enhancement result set; generating a tube body probability graph based on the harmonic selective enhancement result set to obtain a tube body candidate data set; constructing a candidate tube body space-time graph to generate a consistent tube body trajectory set; performing head positioning judgment and reliability evaluation on the consistent tube body trajectory set to determine a jejunum catheterization head positioning result; generating navigation prompt information based on the jejunum catheterization head positioning result, and outputting a navigation interaction result; performing navigation comprehensive risk evaluation on the navigation interaction result, and triggering a closed-loop self-adaption and degradation strategy according to the evaluation result.

The invention belongs to the field of preparation of natural products related to health, and particularly discloses a composition for promoting health of heart and cerebral vessels as well as a preparation method and application of the composition. The composition is prepared from the following raw materials in parts by mass: 10 to 30 parts of earthworm protein, 5 to 20 parts of milk tripeptide, 1 to 10 parts of rice bran fatty alkanol, 5 to 25 parts of aronia melanocarpa extract, 3 to 15 parts of mulberry leaf extract and 1 to 8 parts of micro-embedded lactobacillus plantarum, the composition is prepared into an enteric-coated preparation by a split-phase granulation process: an earthworm protein / lactotripeptide / alkanol coating is an enteric-coated pellet I, an aronia melanocarpa / mulberry leaf extract coating is a sustained-release pellet II, and the enteric-coated pellet I and the sustained-release pellet II are mixed with probiotic pellets and filled into enteric-coated capsules. The system releases protein peptides in duodenum, releases plant extracts slowly in jejunum and releases probiotics in colon, so that the synergistic effects of reducing blood sugar and regulating lipid, resisting atherosclerosis and improving diabetic vasculopathy are remarkably improved.

The invention discloses a pig jejunum crypt separation and organoid model construction method, and belongs to the technical field of animal tissue separation and culture. The method specifically comprises the following steps: intestinal tract collection, cleaning solution preparation, intestinal tract treatment, tissue trimming, tissue cleaning, digestive juice preparation, tissue digestion, cell mass separation, crypt separation, crypt counting, crypt collection, crypt inoculation and organoid culture. The porcine jejunum organ obtained by the method has a typical three-dimensional cystic structure, has complete epithelial polar and functional cell types, including microvillus, goblet cells, panning cells and endocrine cells, and shows good barrier function and drug metabolic activity. A reliable in-vitro model platform is provided for pig intestinal development, nutrient absorption, pathogen infection mechanism research and precision medical treatment, meanwhile, a technical foundation is laid for large-scale application of organoids, and the method has important scientific significance and clinical value.

The present application relates to the field of microbial technology, in particular to bacillus subtilis BS-1 and its application in water buffalo silage feed, the strain BS-1 of the present application has good bacteriostatic activity on escherichia coli, salmonella and streptococcus uberis, after mixing and fermenting according to a specific proportion with orange peel and cassava residue, the bacteriostatic spectrum of the obtained silage feed is significantly enhanced, which can simultaneously inhibit escherichia coli, campylobacter jejuni, salmonella, streptococcus uberis, staphylococcus aureus and streptococcus agalactiae that cause diarrhea and mastitis. In the feed, after mixing 60%-75% of orange peel and 25%-40% of cassava residue, the crude protein content of the fermentation product is significantly improved, and the fiber content is reduced. Feeding test shows that the feed can significantly improve the milk yield and milk quality of lactating water buffalo, and effectively alleviate diarrhea. The present application provides a safe, efficient and nutritious silage feed with disease prevention function.

The invention discloses a combined modular multifunctional stomach decompression jejunum nutrition tube assembly, which relates to the field of medical instruments and comprises a tube body, a first joint, a second joint, a jejunum tube, a suction tube and a pressure adjusting tube. The tube body is formed by connecting a first tube body and a second tube body, the interior of the tube body is divided into a first tube cavity and a second tube cavity which are independent of each other through an arc-shaped partition plate, the first connector and the second connector are inserted into a connector in the top of the tube body in an interchangeable mode, and the suction tube and the pressure adjusting tube penetrate through the first connector to be communicated with the first tube cavity. The second connector is used for fixing a jejunum tube which penetrates through the second tube cavity and is led out from the bottom through hole. Gastrointestinal fluid regurgitation and cross contamination are fundamentally prevented through the tube cavity separation design, the modular structure allows flexible configuration of single or composite functions according to clinical requirements, and meanwhile, the integrated pressing rotary type locking mechanism can stably and conveniently fix and adjust the jejunum tube; and the reliability and the safety of treatment are obviously improved.

The invention discloses a feed additive for improving oxidation resistance of weaned piglets and improving intestinal morphological integrity and application of the feed additive. It is found for the first time that when gamma-PGA of different doses is added into weaned piglet feed, the growth performance of weaned piglets can be remarkably improved, the apparent digestibility of nutrient substances in the feed is improved, the intestinal morphological integrity of small intestines is improved, and jejunum antioxidant enzyme activity and gene expression are promoted. The application of the feed additive is expected to improve the oxidation resistance of the weaned piglets and promote the intestinal morphological integrity of the piglets, and the feed additive has a wide application prospect in actual breeding production. In addition, the invention provides a new solution thought and method for improving animal growth performance and maintaining intestinal morphological integrity.

FIELD: biotechnology.SUBSTANCE: concerns a new strain of bacteria Campylobacter jejuni / 723 of the family Campylobacteriасеае of the genus Campylobacter species C. jejuni, deposited in the All-Russian State Collection of Exotic Types of Foot-and-Mouth Disease Viruses and Other Animal Pathogens (GKSHM) of the FSBI ARRIAH under the registration No. 723-dep / 24-GKSHM of the FSBI ARRIAH. The proposed strain grows on Columbia agar with the addition of 5% defibrinated ram blood; the strain possesses pronounced properties characteristic of its species.EFFECT: presented strain can be used in the development and production of components (antigen and serum) for serological reactions for the diagnosis of campylobacteriosis in farm animals and as a positive control for the identification of bacteria of the genus Campylobacter by real-time PCR and the identification of the species Campylobacter jejuni by time-of-flight mass spectrometry.1 cl, 3 dwgs, 3 tbls, 5 ex

The invention discloses application of beta2M as a biomarker in early weaning stress of animals, and belongs to the technical field of detection and analysis. According to the application of the beta2M as the biomarker in preparation of a product for detecting the early weaning stress of animals, the product is used for detecting the content of the beta2M in jejunum tissues or serum samples and can be used for judging whether the animals have weaning stress symptoms or not. The invention provides a new biomarker for prevention, diagnosis and treatment of animal weaning stress intestinal injury.

The utility model discloses a nasal cavity double-tube fixing device. The nasal cavity double-tube fixing device comprises a fixing tube, a connecting tube and a clamping head. One end of the connecting pipe is fixedly connected with the fixed pipe, and the other end of the connecting pipe is fixedly connected with the clamping head; the jejunum nutrition tube and the pressure reduction tube can be fixed and adjusted through the fixing tube, and specifically, the jejunum nutrition tube and the pressure reduction tube are clamped and loosened through the fixing assembly; compared with a traditional mode that a cloth strip is directly used for fixing, the length of insertion and extraction of the fixed jejunum nutrition tube and decompression tube can be adjusted, and the jejunum nutrition tube and decompression tube can be conveniently suitable for being used by different patients; besides, a clamping head is fixedly arranged at the tail end of the connecting tube, the whole device can be fixed on the nasal cavity of the patient through the clamping head, the fixing tube is directly placed near the nostril, the clamping head is clamped on the nasal cavity of the patient, and the fixing tube is directly fixed and prevented from falling off.

This invention discloses a multilayer microsphere for the prevention and / or treatment of bacterial diarrhea, its preparation method, and its applications, relating to the field of biomedical technology. The multilayer microsphere has a three-layer core-shell structure: the core layer is loaded with *Lactobacillus plantarum* and sodium butyrate, the middle layer is a chitosan coating layer, and the outer layer is a sodium alginate coating layer. This invention has found that *Lactobacillus plantarum* and sodium butyrate have a significant synergistic antibacterial effect, effectively inhibiting the proliferation of pathogenic *Escherichia coli* and *Salmonella*. In vivo experiments have confirmed that the multilayer microsphere can effectively restore the body weight of diarrhea-prone animals, repair pathological damage to the jejunum and colon, and alleviate intestinal inflammation at the molecular level by inhibiting the activation of NF-κB and MAPK inflammatory pathways, downregulating the expression of pro-inflammatory factors IL-1β, IL-6, and TNF-α, and upregulating the level of the anti-inflammatory factor IL-10. The multilayer microsphere provided by this invention can serve as a novel, green, and safe formulation suitable for the prevention and control of bacterial diarrhea in livestock and poultry farming.

The utility model discloses a blockage dredging device for a jejunum tube. The blockage dredging device comprises a three-way joint, a guide core, a vibration handle and a locking piece, the three-head connector comprises a first connector, a second connector and a third connector, the first connector is suitable for being connected with the end of a jejunum tube, the second connector is suitable for being connected with a negative pressure device, and the third connector and the first connector are located on the same axis; one end of the guide core penetrates into the jejunum tube through the third joint and the first joint; an insertion opening is formed in one end of the vibration handle, and the other end of the guide core is detachably inserted into the insertion opening, so that the guide core is driven by the vibration handle to vibrate; the locking piece is arranged at the front end of the vibration handle and used for fixing the other end of the guide core in the inserting opening. The dredging device for the blocked jejunum tube achieves the efficient, safe and convenient dredging effect, and the clinical use value of the jejunum tube and the treatment experience of a patient are remarkably improved.

The application discloses an antibacterial peptide ATMP7 mutant for improving the oxidation stress resistance of animals after an attack and application thereof. The mutants are A7-S1, A7-S2 and A7-S3 with amino acid sequences shown in SEQ ID NO:2, SEQ ID NO:21 and SEQ ID NO:22 respectively. The mutant is convenient to artificially synthesize and low in cost, and has significantly better bacteriostatic activity and application effect than ATMP7, and has better acid resistance. The mutant can improve the oxidation resistance of white-feathered broilers after an attack. The white-feathered broilers are attacked by Clostridium welchii and Escherichia coli, and the oxidation stress reaction of the body is generated, and the mutant can relieve the oxidation stress of the white-feathered broilers after an attack, improve the immunity of the body, restore the morphological structure of the jejunum, regulate the intestinal flora structure, and improve the intestinal barrier function, thereby providing a reference for the application of the antibacterial peptide in breeding.

The utility model discloses a silica gel four-cavity jejunum nutrition tube which comprises a first tube body, a second tube body, an installation head, a feeding channel, a feeding hole, a first ventilation channel, a jejunum balloon, a second ventilation channel, a gastrostomy balloon, a pressure reduction channel, a pressure reduction hole, a feeding tube, a first ventilation tube, a second ventilation tube and a pressure reduction tube. A feeding tube, a first ventilation tube, a second ventilation tube, a pressure reduction tube and a first tube body are fixed on the mounting head, the first tube body is connected with the second tube body, and the mounting head, the feeding tube, the first ventilation tube, the second ventilation tube, the pressure reduction tube, the first tube body and the second tube body are of an integrated structure; according to the jejunum nutrition tube, the pressure reduction hole is formed in the first tube body and communicated with the pressure reduction channel, so that air can be exhausted in the stomach in time when the jejunum nutrition tube is used for feeding, a patient is not prone to feeling uncomfortable due to overlarge pressure in the stomach, and the comfort level of the patient is guaranteed.

The application discloses an albumin postbiotic peptide and application thereof in preparation of a medicine for relieving or treating food allergy, and belongs to the technical field of bioactive peptides. The albumin postbiotic peptide is prepared from egg white protein powder through enzymolysis, inoculation of fermentation lactobacillus mucus IOB802, fermentation and inactivation again. The polysaccharide content in the albumin postbiotic peptide is as high as 1860.74 mg / 100g, the albumin postbiotic peptide can regulate immune balance, reduce allergy-related factors such as IgE and IL-4 and histamine level, increase IgG2a antibody level, reduce infiltration of eosinophils and mast cells in jejunum tissue, down-regulate expression of proinflammatory factors such as IL-25 and TSLP, up-regulate tight junction proteins such as ZO-1 and Occludin, repair intestinal barrier, block penetration of allergens and relieve food allergy symptoms.

The utility model discloses multi -functional visualization is placed pipe soft mirror, including control handle and soft mirror mirror body, one end of soft mirror mirror body is connected with control handle, and the other end of soft mirror mirror body is installed with camera component, the inside installation of casing has battery, battery installation has circuit board, camera component is connected with circuit board, soft mirror mirror body is installed with lighting component, lighting component is connected with circuit board, the outer wall of soft mirror mirror body is recessed to form the recess for accommodating intestinal tube along the axial direction to the inside. Through the recess for accommodating intestinal tube along the axial direction to the inside recessed of the outer wall of soft mirror mirror body, and soft mirror mirror body end installs camera component and lighting component, when placing the tube, the whole embedding recess of presetting intestinal tube of guide wire can realize visualization and guide soft mirror mirror body and intestinal tube to jejunum, after guiding soft mirror mirror body and intestinal tube to jejunum, fix intestinal tube and quit soft mirror, can complete intestinal tube and keep.

The utility model relates to the technical field of jejunum nutrient canals, in particular to a negative pressure suction jejunum nutrient canal which comprises a conveying mechanism, the conveying mechanism comprises a main pipeline and a branch pipe connected to the peripheral side face of the main pipeline, an adjusting ball valve and a drainage mechanism are installed at one end of the branch pipe, and the drainage mechanism comprises a negative pressure ball connected to one end of the main pipeline. One end, far away from the main pipeline, of the negative pressure ball is provided with a connecting pipe, and one end of the connecting pipe is connected with a jejunum nutrient canal; according to the device, the nutrient solution conveying pipe, the negative pressure ball and the drainage bag are arranged, when the device is normally used, a main pipeline can be clamped through a clamp, so that the drainage bag is in a closed state, at the moment, the adjusting ball valve is in an open state, and nutrient solution conveying operation can be normally conducted through the conveying pump; the clamp can be taken down, the adjusting ball valve is closed, at the moment, the negative pressure ball can be pressed to form negative pressure in the negative pressure ball, and therefore foreign matter deposited in the jejunum nutrient canal can be adsorbed.

The present application relates to the field of medical devices, in particular to a guide wire, a guide device and a jejunostomy system, comprising the following components: a main guide wire, which is bendable; a release point, which is located on the main guide wire and can be disconnected to divide the main guide wire into two segments; a capture point, which is located on the main guide wire and is staggered with the release point. The present application can guide the jejunum feeding tube inserted into the stomach cavity to smoothly insert into the jejunum, without cutting the jejunum, greatly reducing the intubation difficulty of the jejunum feeding tube, without causing additional trauma to the patient, avoiding additional gastric stoma hole trauma caused by unsuccessful surgery, and achieving minimally invasive implantation with a simple implantation process.

The invention discloses a campylobacter jejuni liquid-phase probe targeted capturing and library building method and a kit thereof, and belongs to the technical field of germ detection. Specific probe enrichment, magnetic bead purification and high-throughput sequencing are combined, and the problems that in the prior art, low-load detection is difficult, information is incomplete, and operation is tedious are solved. Aiming at the characteristics of wide food-borne transmission, multiple low-load samples and strong complex matrix interference of campylobacter jejuni, low-load accurate detection, whole-genome information analysis and high-throughput standardized operation are realized through a liquid-phase probe targeted capture technology; the method is suitable for large-scale sample detection in the fields of food safety detection, clinical diagnosis, public health research and the like.

This invention discloses a monoclonal antibody for detecting the surface protein SapA of Campylobacter fetus and its application. The monoclonal antibodies are named 4F6 and 7B3, and the amino acid sequences of the heavy and light chain variable regions of these two antibodies are disclosed. The monoclonal antibody prepared by this invention can specifically recognize the surface protein SapA of Campylobacter fetus. The prepared monoclonal antibody has good specificity, showing no cross-reactivity with non-Camplybacteria such as standard Escherichia coli, Salmonella, Listeria, Klebsiella pneumoniae, Staphylococcus aureus, and Streptococcus, and also with Campylobacter jejuni and Campylobacter coli. The test strip prepared by combining the monoclonal antibody of this invention with colloidal gold can achieve rapid on-site detection of Campylobacter fetus with a low detection limit and high sensitivity, successfully developing an immunological method for the specific detection of Campylobacter fetus.