199 results about "Jejunum" patented technology

The invention belongs to the technical field of microbial genetic engineering, and particularly relates to an alpha-2, 3-sialyltransferase mutant and application thereof. The a-2, 3-sialyltransferase mutants V175N, P198A, G173D, D174Q, F196K and A315H are obtained by performing site-specific mutagenesis on six amino acids in an a-2, 3-sialyltransferase amino acid sequence coded by a gene derived from Campylobacter jejuni (OH4384), the yield of 3 '-sialyllactose is remarkably increased by single-point mutagenesis of the V175N, the yield of 3'-sialyllactose is remarkably increased by single-point mutagenesis of the V175N, the yield of 3 '-sialyllactose is remarkably increased by single-point mutagenesis of the P198A, the G173D, the D174Q, the F196K and the A315H, the optimal recombinant microbial strain 3 '-SL-09 (V175N / P198A / D174Q) is obtained through combination of multiple mutation sites, the yield can reach 5.91 g / L, the bottleneck problem of low catalytic efficiency of sialyltransferase is effectively solved, and an enzyme element with excellent performance is provided for high-efficiency and low-cost biological manufacturing of 3'-SL.

The utility model provides a nasogastroscope-guided gastrointestinal catheterization system based on a guide wire. The nasogastroscope-guided gastrointestinal catheterization system comprises a nasogastroscope (1), an imbedding tube (2) and the guide wire (3), wherein the guide wire (3) is arranged in an instrument cavity of the nasogastroscope (1); the implantation tube (2) is arranged along the guide wire (3) to a target jejunum nutrition tube putting position; the diameter of the guide wire (3) is 0.4 mm to 1.8 mm; the length of the guide wire is 1-7 m, and the section, 2-8 cm long, of the front end of the guide wire (3) is a flexible section (3.9). The utility model has the advantages of simple structure, simplification of catheterization operation, reduction of the pain of a patient, reduction of medical risks, guarantee of the success rate of catheterization, and excellent technical effect; the method has huge economic value and social value.

The invention belongs to the field of microorganisms and fermentation, and relates to a biological preparation, a preparation method thereof and application of the biological preparation in preparation of products for inhibiting escherichia coli. And the biological agent is a co-fermentation product of fructus forsythiae and bacillus amyloliquefaciens (Bacillus amyloliquefaciens). The biological preparation provided by the invention can obviously improve the secretion of IFN-alpha in a mouse body, reduce the production of IL-1beta, IL-6 and TNF-alpha, increase the proportion of CD3 + CD4 + and CD3 + CD8 + T cells, and improve the expression of ZO-1 and Occludin in duodenum and jejunum. Compared with single use of fructus forsythiae and bacillus amyloliquefaciens, the biological preparation shows a stronger synergistic effect, can effectively prevent and inhibit escherichia coli infection, and is not easy to generate drug resistance. The preparation method is simple, raw materials are easy to obtain, and the feed additive is suitable for developing antibacterial drugs and feed additives.

The invention relates to the technical field of guiding devices, in particular to a nasal jejunum nutrition tube imbedding guiding device which is used for guiding the process of imbedding a flexible nasal jejunum tube into a human body. The image acquisition device acquires a picture in the direction of the advancing direction of the insertion end of the nasal jejunum tube; the bag body is arranged in a manner of extending close to the insertion end of the nasal jejunum tube; during use, the bag body does not generate binding force on the nasal jejunum tube in a low-expansion state, the shape of the nasal jejunum tube is not limited by the bag body, when the bag body is in a high-expansion state, the bag body generates binding force on the nasal jejunum tube to enable the nasal jejunum tube to be bent, and the image acquisition device comprises a camera arranged in the insertion end of the nasal jejunum tube. The insertion end of the nasointestinal tube is provided with a closing part for preventing the camera from falling off, and the problem that the throat and the pylorus cannot pass through the nasointestinal tube quickly and smoothly in the existing nasointestinal tube indwelling process can be solved by improving the existing endoscope guiding technology.

The invention relates to the technical field of medical robots, in particular to a three-dimensional reconstruction method and system for a nasal jejunum catheterization robot. The method comprises the following steps: acquiring endoscope image data in real time through an endoscope camera and a data transmission channel, and performing preliminary denoising and enhancement; a parallel processing architecture is adopted, and the endoscope image data is divided, subjected to image denoising and enhancement again, improved in image contrast and subjected to distortion correction processing in sequence; carrying out dynamic modeling on the newly collected image data in the nasal jejunum dynamic three-dimensional model; performing dynamic path planning and attitude adjustment, and detecting errors in real time; the system comprises a data acquisition module, a data parallel processing module, a dynamic modeling module and an adjusting module. In this way, the real-time performance, the soft tissue adaptability and the catheter positioning information precision can be met, and therefore the reliability of nasal jejunum catheterization operation is improved.

The utility model discloses a jejunum catheterization human body model teaching aid, comprising a human body model which is internally provided with a catheterization channel which simulates an implantation path of a bionic human body nasointestinal tube; the human body model and the catheter placing channel are made of transparent materials. Therefore, the complete digestive tract structure from the oral cavity to the jejunum of the human body can be simulated, the whole process of nasointestinal tube placement can be visually and dynamically displayed, and the effect of jejunum tube placement teaching and practice training is improved.

The invention relates to the technical field of microorganisms, in particular to ruminococcus buchneri and application of in-vitro pure culture metabolic supernate of the ruminococcus buchneri in promoting intestinal development of piglets. The invention relates to ruminococcus buchneri, and the collection number of the ruminococcus buchneri is ATCC (American Type Culture Collection) 51896. The ruminococcus buchneri and the in-vitro pure culture metabolic supernate thereof can obviously improve the unit intestinal weight of the cecum of the weaned piglets, and have the trend of increasing the unit intestinal weight of the jejunum and the ileum of the piglets, increasing the average daily feed intake and reducing the feed-to-gain ratio. The in-vitro organoid culture result also shows that the amplification efficiency of the jejunum organoid of the piglet is remarkably improved.

The invention discloses bifidobacterium bifidum and application of the bifidobacterium bifidum in relieving ovalbumin-induced food allergy, and belongs to the technical field of microorganisms. According to the invention, a strain of bifidobacterium bifidum CCFM1063 is screened out, and the bifidobacterium bifidum CCFM1063 has the effects of relieving weight loss and allergic symptoms of mice caused by OVA; according to the present invention, with the application of the loratadine, the high mMCP-1 level caused by OVA can be significantly reduced, the IgE level can be reduced, the mRNA level of the jejunum allergy related cytokines such as TSLP, IL-4 and IL-33 can be significantly reduced, and the partial effect is superior to the loratadine; when the bifidobacterium bifidum CCFM1063 is combined with breast milk oligosaccharide 3 '-SL, the bifidobacterium bifidum CCFM1063 has a synergistic effect on improving food allergy symptoms, and the effect of the bifidobacterium bifidum CCFM1063 is obviously superior to that of a single strain.

The invention relates to the technical field of microorganisms, in particular to a lactobacillus plantarum strain LM2 and application of the lactobacillus plantarum strain LM2. The strain has relatively high GABA (gamma-aminobutyric acid) production capacity and nitrite degradation capacity, has a bacteriostatic effect on intestinal pathogens such as staphylococcus aureus, salmonella enteritidis, listeria monocytogenes and campylobacter jejuni, has a good bacteriostatic function, also has good acid resistance and cholate resistance, and can be used for preparing a bacterial strain for treating intestinal diseases. The strain can well survive in artificial intestinal juice and artificial gastric juice; in order to increase the yield of GABA, the accelerant capable of remarkably promoting the growth of the strain LM2 is obtained by researching the accelerant, and the highest survival rate of the freeze-dried powder prepared by optimizing the accelerant and the freeze-dried powder can reach 99% or above, the nitrite degradation rate can reach 99% or above, the yield of GABA is increased to 3.0 g / L or above, and the freeze-dried powder is bacterial powder capable of being widely applied.

The invention discloses bifidobacterium breve with hypoglycemic capacity and application, a product and a method thereof, and belongs to the technical field of microorganisms. The invention provides a bifidobacterium breve (Bifidobacterium breve) strain BBr37 with the blood sugar reducing capacity, and the preservation number of the bifidobacterium breve strain BBr37 is CGMCC (China General Microbiological Culture Collection Center) NO.32423. The invention also provides an application of the strain BBr37 in preparation of hypoglycemic drugs or health-care foods with an auxiliary hypoglycemic function or bacteriostatic agents or non-treatment-destination bacteriostasis, and a product and a method based on the strain BBr37. The strain BBr37 disclosed by the invention has a relatively strong inhibition effect on escherichia coli, staphylococcus aureus, salmonella, haemophilus parasuis, haemophilus parainfluenza, campylobacter jejuni and enterocolitis yarrowia, and can effectively achieve the effects of reducing blood sugar and regulating intestinal flora.

The invention relates to an aleppo avens n-acetone extract and application thereof in treating necrotic enteritis. The aleppo avens n-acetone extract is obtained through a reflux extraction method, and the extraction conditions are as follows: the acetone concentration is 50% or 75%, the reaction time is 0.5 h or 1 h, the material-liquid ratio is 1: 5 or 1: 10, and the extraction temperature is 65 DEG C. According to the invention, a reflux extraction method is adopted to extract the aleppo avens, and the obtained aleppo avens n-acetone extract contains various compounds such as ellagic acid, buddleoside, 4, 4 '-dimethyl ellagic acid and the like; the aleppo avens n-acetone extract can increase the villus height of duodenum and jejunum and the ratio of villus height to crypt depth, maintain the morphological structure in the duodenum intestinal tract, increase the content of acetic acid, propionic acid and butyric acid in cecum, and significantly reduce necrotic enteritis lesion.

The invention belongs to the technical field of biological medicines, and particularly relates to application of UH15-38 in preparation of a medicine for preventing and treating PEDV-PoRVA co-infection. According to the invention, a PEDV and PoRVA co-infected piglet model is systematically established for the first time, a high-throughput transcriptomics means is combined, an enhancement mechanism of co-infection on host pathogenicity is deeply analyzed from an immune regulation level, and based on the mechanism, UH15-38 is found to be capable of effectively treating PEDV and PoRVA co-infected piglets. Experimental data show that the UH15-38 remarkably relieves clinical diarrhea symptoms caused by co-infection, reduces the copy number of PoRVA and PEDV viruses, effectively inhibits duplication of the viruses in intestinal tracts, remarkably reduces the expression levels of IL-1beta and p-MLKL in jejunum tissues, and reduces necrotic apoptosis and inflammatory response. The UH15-38 can provide a new application value for the treatment of the porcine viral diarrhea.

The invention discloses a kit for simultaneously detecting 16 food-borne pathogenic bacteria. Each group of probes is designed based on bacterial classification, virulence gene specificity or serotype marks of the pathogenic bacteria; a group A probe vibrio detection group is used for detecting vibrio cholerae universal type, vibrio cholerae O1 serotype, vibrio cholerae O139 serotype and vibrio parahaemolyticus; the B group of probe invasive enterobacteriaceae pathogen group is used for detecting escherichia coli O157: H7, shigella, salmonella and yersinia small intestine; the C group probe escherichia coli pathotype detection group is used for detecting enterotoxigenic escherichia coli ETEC, enteroinvasive escherichia coli EIEC, enteroaggregative adherent escherichia coli EAEC and pathogenic escherichia coli EPEC; and the D group of probes are used for detecting campylobacter jejuni, campylobacter colons, listeria monocytogenes and enterohemorrhagic escherichia coli EHEC in the campylobacter and toxin type pathogen group.

The invention discloses a probiotic biofilm pulse ultrasonic atomization spraying device, and particularly relates to the technical field of jejunum nutrient canal processing.The device comprises a base, and a spraying sleeve shell and an electric frame are installed at the top of the base; an inner conversion frame is rotationally arranged in the spraying sleeve shell, a linear placement groove is formed in the outer side of the inner conversion frame, and multiple sets of vacuum fixing clamps are slidably connected to the inner walls of the two sides of the linear placement groove; a rotary drawing mechanism is arranged in the electrical frame and comprises a fixed feeding pipe, a drawing sleeve and an air supply shaft seat; the drawing sleeve penetrates through the middle of the air supply shaft seat and is in threaded connection with the air supply shaft seat, the air supply shaft seat is provided with a second cooling pipeline and an air outlet ring cavity from inside to outside, a first communicating hose is connected between the second cooling pipeline and the fixed feeding pipe, a vortex sheet is arranged in the air outlet ring cavity, and the air outlet ring cavity can be filled with inert gas; and piezoelectric ultrasonic atomization sheets are mounted in the end parts of the cooling pipeline II and the drawing sleeve. Environmental microbial pollution is effectively prevented, and the spraying effect of the probiotic biological membrane is guaranteed.

The invention discloses application of bifidobacterium longum subsp. Infantis CCFM1269 in preparation of a product for relieving lactoglobulin allergy, and belongs to the technical field of microorganisms. The bifidobacterium longum subsp. Infantis CCFM1269 provided by the invention can relieve food allergy, and the food allergy can be relieved specifically as follows: (1) allergy symptom score and diarrhea occurrence rate can be relieved, and anus temperature and immune organ index can be recovered; (2) the immune response is regulated, the levels of histamine, total IgE, IgG1, beta-lactoglobulin specific IgE and proinflammatory factors IL-4 and IL-17A in serum are reduced, the levels of IFN-gamma and IL-10 are improved, and the immune balance is recovered; (3) inflammation of lung and jejunum tissues is relieved; (4) improving the oxidative stress state; (5) the intestinal barrier function is enhanced; and (6) recovering the diversity of intestinal flora, and adjusting the relative abundance of key bacteria genus such as Alloprevotella and the like.

The utility model discloses a nasal jejunum endoscope which comprises a nasal tube, the upper end of the nasal tube is communicated with a connector used for connection, and the two ends of the connector are respectively provided with a branch tube capable of penetrating into a jejunum nutrient canal or a nasobiliary inner tube. The bullet head is connected to the end, away from the connector, of the nasal tube in a sleeved mode, a through hole is formed in the surface of the bullet head, and the through hole is communicated with the nasal tube and the connector. When the nasal jejunointestinal tube endoscope is used, the LED lamp core is turned on to position the nasal jejunointestinal tube, blind insertion is not needed, the intubation risk is reduced, pain of a patient is relieved, meanwhile, the LED lamp core and the lamp holder are wrapped by the omni-directional silica gel sleeve, the possibility that seepage flows in is prevented, safety and reasonability of the whole structure are guaranteed, the whole nasal tube, the connector, the branch tube and the bullet head form the nasal jejunointestinal tube, and the nasal jejunointestinal tube endoscope is convenient to use. The nasal tube can be smoothly introduced into the gastrointestinal part through the end part of the bullet head, and meanwhile, the hollow space of the bullet head has a small-amplitude buffer, so that the possibility of hard injury to the stomach wall and the esophageal wall is reduced.

The invention relates to the technical field of medical instrument simulation and testing, in particular to a high-precision 3D printing personalized alimentary canal entity model method which comprises the steps that alimentary canal CT image data covering the range from the nasal cavity to the jejunum of a target individual and biomechanical property reference data of different sections of the alimentary canal are obtained; generating a stiffness distribution diagram; transparent flexible photosensitive resin is used as a base material, and various transparent flexible photosensitive resin materials with different elastic moduli are prepared by adding a modifier; generating a printing instruction; a digital light processing 3D printer is adopted, a solid model is printed layer by layer according to a printing instruction, the distributed transparent flexible photosensitive resin material is automatically switched and used in the printing process, and exposure parameters of ultraviolet light are dynamically adjusted based on the characteristics of the currently used transparent flexible photosensitive resin material so as to control the post-curing elastic modulus of different sections. Through accurate elastic modulus control and dynamic exposure adjustment, the mechanical properties of different sections of the digestive tract are highly consistent with the actual situation.

The utility model discloses an anti-displacement pancreatic duct support, and relates to the technical field of medical instruments. The anti-displacement pancreatic duct support comprises a traction ball, a flow guide structure is arranged on the outer side of the traction ball, an anti-displacement structure is arranged at the other end of the flow guide structure, and a support structure is arranged on the other side of the anti-displacement structure; according to the anti-displacement pancreatic duct stent, the anti-displacement structure is arranged, the flow guide structure and the stent structure can be connected through the two connecting joints, and then the anti-displacement pancreatic duct stent is sewn and fixed to the jejunum mucous membrane surface of a pancreatic anastomotic stoma through an absorbable suture in cooperation with a suture hole in the fixing blocking piece; the function of reinforcing the anastomotic stoma and reducing erosion of the anastomotic stoma by digestive juice is achieved, the situation that the stent accidentally falls off in the pancreatic duct is avoided, the stability of the stent is improved, and meanwhile the use efficiency of the stent is improved.

The invention discloses a rapeseed-derived polypeptide capable of relieving injury of vomitoxin to piglet intestines and application of the rapeseed-derived polypeptide. The rapeseed polypeptide provided by the invention is prepared from the following peptide fragments: YLKDPSKF, IVGI, KGFF, GHQYIVT, YYF, FHLY, STFSEY, SVDKQIAK, FYSI and ALRAPLGQN. The rapeseed polypeptide has the advantages that the rapeseed polypeptide is prepared from the following peptide fragments; the method comprises the following steps: extracting rapeseed protein isolate from fat rapeseed dregs, and carrying out enzymolysis, dialysis and ultrafiltration to obtain the micromolecular rapeseed polypeptide with the molecular weight of less than 1kDa. Researches show that the polypeptide can relieve piglet weight loss caused by vomitoxin intestinal injury, can reduce the concentration of proinflammatory factors in serum, and can relieve inflammatory factor infiltration; meanwhile, the rapeseed polypeptide can protect the jejunum tissue of the piglet and reverse jejunum tissue crypt hypertrophy and intestinal villus shortening caused by a DON model group, has an intestinal tissue injury recovery effect, and can be applied to deep processing of rapeseed meal and relieving vomitoxin-induced piglet intestinal injury.

The utility model discloses a mould is placed under the mould of simulation ultrasonic real -time guidance of nasal -intestinal tube, including the digestive tract simulation body of simulation esophagus, stomach, duodenum and jejunum, it adopts the flexible material of ultrasonic wave penetration, front surface is not transparent, back surface is transparent, and its inside esophagus is used for filling air, and the part except esophagus is used for filling liquid, the digestive tract simulation body is fixed between two organic glass, front surface is not transparent, back surface is transparent, the organic glass of front surface opens at esophagus place, antrum gastricum place respectively, as the acoustic window of ultrasonic, is used for the inflation sign of esophagus and antrum gastricum nasal -intestinal tube catheter tip cloud sign of ultrasonic observation when placing nasal -intestinal tube respectively, is provided with many resistance positions at stomach and duodenum, for making nasal -intestinal tube catheter tip forward into the obstruction. The utility model can simulate the sign of ultrasonic under different conditions on the mould, effectively simulates the actual entering process of nasal -intestinal tube under the real -time guidance of ultrasonic, and facilitates simulation teaching demonstration.

A duodenum built-in jejunum cannula releasing system and a use method therefor, the system including: implanting device, pushing assembly, fixing line and releasing line. The implanting device includes storage pipe shell, releaser, membrane pipe and stent; the storage pipe shell is tubular and provided with near-end opening and far-end opening; the membrane pipe and the stent which are to be released are arranged in the storage pipe shell in folded state; the pushing assembly includes push-pull guiding wire, inner pipe, middle pipe and outer pipe which are sleeved with one another from the inside out in sequence and may move relative to one another.

1. The name of the product of this design: Duodenal jejunal internal cannula device. 2. Purpose of the product of this design: used for the insertion and removal of medical products in the digestive tract. 3. The design key point of this appearance design product lies in the shape. 4. The picture or photo that best illustrates the key points of the design: 3D drawing of Design 1. 5. Designate Design 1 as the base design. 6. The product of this design is a slender object, and its length is omitted.

The application discloses a nasal jejunum catheterization robot and a system thereof, and relates to the technical field of medical devices.The robot control box is connected with a nasal jejunum tube at the output end, and one end of the nasal jejunum tube is provided with a robot end part.The inside of the robot end part is provided with a steering assembly, and one end of the steering assembly is mounted with an electromagnetic chuck.The one end of the electromagnetic chuck is connected with one side of the tubular device through magnetic attraction.The input end of the electromagnetic chuck is electrically connected with a wire harness.The one end of the wire harness penetrates through the inside of the nasal jejunum tube and is connected with the inside control end of the robot control box.The robot end part comprises a leather sheath, and a plurality of holes are formed in the outer wall of the leather sheath.A plurality of sensors are mounted in the holes respectively, and the sensors are electrically connected with the wire harness respectively.

This invention discloses a fermented microbial preparation for inhibiting Campylobacter jejuni in calves, its preparation method, and its application. The fermented microbial preparation is a co-fermentation product of a mixture of Lygodium japonicum decoction and liquid culture medium, inoculated with Lactobacillus paracasei; the Lactobacillus paracasei is... Lacticaseibacillus paracasei KQNIU. Comparative experiments in this invention revealed that, compared to other types of traditional Chinese medicine, the inhibitory effect of fermented Lygodium japonicum on Campylobacter jejuni is significantly higher than that of other types of traditional Chinese medicine. Furthermore, the co-fermentation of the traditional Chinese medicine Lygodium japonicum and the probiotic Lactobacillus paracasei KQNIU achieves a synergistic effect between the two, overcoming the problem of limited efficacy when used alone.

1. Name of the product in this design: Anastomosis device (inserted fully endocavitary electrically powered tubular type - straight). 2. Application of this design: for laparoscopic anastomosis of esophagus and jejunum, intestine to intestine, or thoracoscopic anastomosis of esophagus and jejunum or esophagus and stomach within the thoracic cavity. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D view 1. 5. Other situations requiring explanation: Part A is made of transparent material.

The invention relates to the technical field of biological agriculture, in particular to a preparation method, device and application of basic ionic liquid for animals, and the basic ionic liquid comprises potassium bicarbonate, potassium carbonate, sodium bicarbonate, sodium carbonate, germanium dioxide, sodium selenite, chromic oxide, chitosan oligosaccharide and purified water. The preparation method and device of the basic ionic liquid for animals and the application have the beneficial effects that through scientific proportioning and careful preparation, the basic ionic liquid can remarkably improve the growth performance, the immunity and the intestinal health level of animals, improve the villus height and the crypt depth of jejunum and ileum, and improve the animal immunity. And a ratio of fluff length to recess depth; and by optimizing the equipment structure and the operation process, the preparation efficiency and quality are improved, and the labor cost and the error rate are reduced.

The utility model provides an improved jejunum fistulization tube, and relates to the technical field of fistulization tubes, the improved jejunum fistulization tube comprises a fistulization tube body, the fistulization tube body comprises a tube body used for feeding, the tube body comprises a feeding section and a discharging hole, and the feeding section is arranged at one end of the tube body; the discharging hole is formed in the side face, away from the feeding section, of the pipe body, so that the nutrient solution can flow in from the feeding section and flow out from the discharging hole; the discharge hole is in a long strip shape along the axis of the pipe body; the long-strip-shaped discharging holes are formed in the end, extending into the jejunum, of the tube body, so that a nutrient solution in the tube body flows into the jejunum from the discharging holes, when particles in the nutrient solution block the discharging holes, the discharging holes have enough space so that the particles can flow into the jejunum from the discharging holes in different positions, and the blocking risk is reduced.

1. The name of the product of this design: Esophageal jejunal stent (bristle type). 2. Purpose of the product of this design: to prevent or treat anastomotic fistula after reconstruction and anastomosis of the digestive tract in the human body. 3. The key points of the design of this product are its shape and its combination of elements. 4. The picture or photo that best illustrates the key points of the design: main view.

The utility model provides a jejunum fistulization tube fixing application, which belongs to the technical field of medical instruments and comprises an absorption layer (1), a through hole (2) matched with a fistulization tube is formed in the absorption layer (1), an opening (3) is formed in the absorption layer (1), and the opening (3) is communicated with the through hole (2); the absorbing layer (1) is detachably connected with a waterproof layer (4) matched with the absorbing layer (1); the waterproof layer (4) and the absorption layer (1) are attached to each other and then press and fix the fistulization tube. A bonding layer (5) is arranged on the periphery of the absorbing layer (1); a plurality of groups of bandages (6) are symmetrically arranged on two sides of the opening (3) of the absorption layer (1); an edge sealing strip (7) is arranged on one side of the opening (3) of the absorption layer (1), stretches across the opening (3) and is adhered to the other side of the opening (3); the application provided by the utility model is convenient for a patient to turn over and wear, and can prevent external liquid from permeating into a fistulization opening to cause infection when the body of the patient is cleaned.

The invention discloses a detection method for detecting enteropathogenic bacteria, and relates to the field of molecular biology, specific genes are searched in genomes of salmonella, shigella, vibrio parahaemolyticus, campylobacter jejuni, diarrheagenic escherichia coli, vibrio cholerae and staphylococcus aureus, a beta-actin gene is adopted as a reference gene, and the enteropathogenic bacteria are detected. Primer and probe sequences are designed, a probe group consisting of the primer and probe sequences is used for carrying out real-time fluorescent PCR amplification reaction on nucleic acid in a detected sample, then melting curve analysis is carried out, and whether the pathogenic bacteria exist in the nucleic acid sequence to be detected or not can be judged according to a Tm value. The detection method provided by the invention has the advantages of rapidness, high throughput, accuracy and high sensitivity.