145 results about "Jejunum" patented technology

The invention belongs to the technical field of microbial genetic engineering, and particularly relates to an alpha-2, 3-sialyltransferase mutant and application thereof. The a-2, 3-sialyltransferase mutants V175N, P198A, G173D, D174Q, F196K and A315H are obtained by performing site-specific mutagenesis on six amino acids in an a-2, 3-sialyltransferase amino acid sequence coded by a gene derived from Campylobacter jejuni (OH4384), the yield of 3 '-sialyllactose is remarkably increased by single-point mutagenesis of the V175N, the yield of 3'-sialyllactose is remarkably increased by single-point mutagenesis of the V175N, the yield of 3 '-sialyllactose is remarkably increased by single-point mutagenesis of the P198A, the G173D, the D174Q, the F196K and the A315H, the optimal recombinant microbial strain 3 '-SL-09 (V175N / P198A / D174Q) is obtained through combination of multiple mutation sites, the yield can reach 5.91 g / L, the bottleneck problem of low catalytic efficiency of sialyltransferase is effectively solved, and an enzyme element with excellent performance is provided for high-efficiency and low-cost biological manufacturing of 3'-SL.

The utility model provides a nasogastroscope-guided gastrointestinal catheterization system based on a guide wire. The nasogastroscope-guided gastrointestinal catheterization system comprises a nasogastroscope (1), an imbedding tube (2) and the guide wire (3), wherein the guide wire (3) is arranged in an instrument cavity of the nasogastroscope (1); the implantation tube (2) is arranged along the guide wire (3) to a target jejunum nutrition tube putting position; the diameter of the guide wire (3) is 0.4 mm to 1.8 mm; the length of the guide wire is 1-7 m, and the section, 2-8 cm long, of the front end of the guide wire (3) is a flexible section (3.9). The utility model has the advantages of simple structure, simplification of catheterization operation, reduction of the pain of a patient, reduction of medical risks, guarantee of the success rate of catheterization, and excellent technical effect; the method has huge economic value and social value.

The invention belongs to the field of microorganisms and fermentation, and relates to a biological preparation, a preparation method thereof and application of the biological preparation in preparation of products for inhibiting escherichia coli. And the biological agent is a co-fermentation product of fructus forsythiae and bacillus amyloliquefaciens (Bacillus amyloliquefaciens). The biological preparation provided by the invention can obviously improve the secretion of IFN-alpha in a mouse body, reduce the production of IL-1beta, IL-6 and TNF-alpha, increase the proportion of CD3 + CD4 + and CD3 + CD8 + T cells, and improve the expression of ZO-1 and Occludin in duodenum and jejunum. Compared with single use of fructus forsythiae and bacillus amyloliquefaciens, the biological preparation shows a stronger synergistic effect, can effectively prevent and inhibit escherichia coli infection, and is not easy to generate drug resistance. The preparation method is simple, raw materials are easy to obtain, and the feed additive is suitable for developing antibacterial drugs and feed additives.

The invention relates to the technical field of guiding devices, in particular to a nasal jejunum nutrition tube imbedding guiding device which is used for guiding the process of imbedding a flexible nasal jejunum tube into a human body. The image acquisition device acquires a picture in the direction of the advancing direction of the insertion end of the nasal jejunum tube; the bag body is arranged in a manner of extending close to the insertion end of the nasal jejunum tube; during use, the bag body does not generate binding force on the nasal jejunum tube in a low-expansion state, the shape of the nasal jejunum tube is not limited by the bag body, when the bag body is in a high-expansion state, the bag body generates binding force on the nasal jejunum tube to enable the nasal jejunum tube to be bent, and the image acquisition device comprises a camera arranged in the insertion end of the nasal jejunum tube. The insertion end of the nasointestinal tube is provided with a closing part for preventing the camera from falling off, and the problem that the throat and the pylorus cannot pass through the nasointestinal tube quickly and smoothly in the existing nasointestinal tube indwelling process can be solved by improving the existing endoscope guiding technology.

The invention relates to the technical field of medical robots, in particular to a three-dimensional reconstruction method and system for a nasal jejunum catheterization robot. The method comprises the following steps: acquiring endoscope image data in real time through an endoscope camera and a data transmission channel, and performing preliminary denoising and enhancement; a parallel processing architecture is adopted, and the endoscope image data is divided, subjected to image denoising and enhancement again, improved in image contrast and subjected to distortion correction processing in sequence; carrying out dynamic modeling on the newly collected image data in the nasal jejunum dynamic three-dimensional model; performing dynamic path planning and attitude adjustment, and detecting errors in real time; the system comprises a data acquisition module, a data parallel processing module, a dynamic modeling module and an adjusting module. In this way, the real-time performance, the soft tissue adaptability and the catheter positioning information precision can be met, and therefore the reliability of nasal jejunum catheterization operation is improved.

The utility model discloses a jejunum catheterization human body model teaching aid, comprising a human body model which is internally provided with a catheterization channel which simulates an implantation path of a bionic human body nasointestinal tube; the human body model and the catheter placing channel are made of transparent materials. Therefore, the complete digestive tract structure from the oral cavity to the jejunum of the human body can be simulated, the whole process of nasointestinal tube placement can be visually and dynamically displayed, and the effect of jejunum tube placement teaching and practice training is improved.

The invention relates to the technical field of microorganisms, in particular to ruminococcus buchneri and application of in-vitro pure culture metabolic supernate of the ruminococcus buchneri in promoting intestinal development of piglets. The invention relates to ruminococcus buchneri, and the collection number of the ruminococcus buchneri is ATCC (American Type Culture Collection) 51896. The ruminococcus buchneri and the in-vitro pure culture metabolic supernate thereof can obviously improve the unit intestinal weight of the cecum of the weaned piglets, and have the trend of increasing the unit intestinal weight of the jejunum and the ileum of the piglets, increasing the average daily feed intake and reducing the feed-to-gain ratio. The in-vitro organoid culture result also shows that the amplification efficiency of the jejunum organoid of the piglet is remarkably improved.

The invention relates to the technical field of microorganisms, in particular to a lactobacillus plantarum strain LM2 and application of the lactobacillus plantarum strain LM2. The strain has relatively high GABA (gamma-aminobutyric acid) production capacity and nitrite degradation capacity, has a bacteriostatic effect on intestinal pathogens such as staphylococcus aureus, salmonella enteritidis, listeria monocytogenes and campylobacter jejuni, has a good bacteriostatic function, also has good acid resistance and cholate resistance, and can be used for preparing a bacterial strain for treating intestinal diseases. The strain can well survive in artificial intestinal juice and artificial gastric juice; in order to increase the yield of GABA, the accelerant capable of remarkably promoting the growth of the strain LM2 is obtained by researching the accelerant, and the highest survival rate of the freeze-dried powder prepared by optimizing the accelerant and the freeze-dried powder can reach 99% or above, the nitrite degradation rate can reach 99% or above, the yield of GABA is increased to 3.0 g / L or above, and the freeze-dried powder is bacterial powder capable of being widely applied.

The invention belongs to the technical field of biological medicines, and particularly relates to application of UH15-38 in preparation of a medicine for preventing and treating PEDV-PoRVA co-infection. According to the invention, a PEDV and PoRVA co-infected piglet model is systematically established for the first time, a high-throughput transcriptomics means is combined, an enhancement mechanism of co-infection on host pathogenicity is deeply analyzed from an immune regulation level, and based on the mechanism, UH15-38 is found to be capable of effectively treating PEDV and PoRVA co-infected piglets. Experimental data show that the UH15-38 remarkably relieves clinical diarrhea symptoms caused by co-infection, reduces the copy number of PoRVA and PEDV viruses, effectively inhibits duplication of the viruses in intestinal tracts, remarkably reduces the expression levels of IL-1beta and p-MLKL in jejunum tissues, and reduces necrotic apoptosis and inflammatory response. The UH15-38 can provide a new application value for the treatment of the porcine viral diarrhea.

The invention discloses a kit for simultaneously detecting 16 food-borne pathogenic bacteria. Each group of probes is designed based on bacterial classification, virulence gene specificity or serotype marks of the pathogenic bacteria; a group A probe vibrio detection group is used for detecting vibrio cholerae universal type, vibrio cholerae O1 serotype, vibrio cholerae O139 serotype and vibrio parahaemolyticus; the B group of probe invasive enterobacteriaceae pathogen group is used for detecting escherichia coli O157: H7, shigella, salmonella and yersinia small intestine; the C group probe escherichia coli pathotype detection group is used for detecting enterotoxigenic escherichia coli ETEC, enteroinvasive escherichia coli EIEC, enteroaggregative adherent escherichia coli EAEC and pathogenic escherichia coli EPEC; and the D group of probes are used for detecting campylobacter jejuni, campylobacter colons, listeria monocytogenes and enterohemorrhagic escherichia coli EHEC in the campylobacter and toxin type pathogen group.

The invention discloses a probiotic biofilm pulse ultrasonic atomization spraying device, and particularly relates to the technical field of jejunum nutrient canal processing.The device comprises a base, and a spraying sleeve shell and an electric frame are installed at the top of the base; an inner conversion frame is rotationally arranged in the spraying sleeve shell, a linear placement groove is formed in the outer side of the inner conversion frame, and multiple sets of vacuum fixing clamps are slidably connected to the inner walls of the two sides of the linear placement groove; a rotary drawing mechanism is arranged in the electrical frame and comprises a fixed feeding pipe, a drawing sleeve and an air supply shaft seat; the drawing sleeve penetrates through the middle of the air supply shaft seat and is in threaded connection with the air supply shaft seat, the air supply shaft seat is provided with a second cooling pipeline and an air outlet ring cavity from inside to outside, a first communicating hose is connected between the second cooling pipeline and the fixed feeding pipe, a vortex sheet is arranged in the air outlet ring cavity, and the air outlet ring cavity can be filled with inert gas; and piezoelectric ultrasonic atomization sheets are mounted in the end parts of the cooling pipeline II and the drawing sleeve. Environmental microbial pollution is effectively prevented, and the spraying effect of the probiotic biological membrane is guaranteed.

The invention discloses application of bifidobacterium longum subsp. Infantis CCFM1269 in preparation of a product for relieving lactoglobulin allergy, and belongs to the technical field of microorganisms. The bifidobacterium longum subsp. Infantis CCFM1269 provided by the invention can relieve food allergy, and the food allergy can be relieved specifically as follows: (1) allergy symptom score and diarrhea occurrence rate can be relieved, and anus temperature and immune organ index can be recovered; (2) the immune response is regulated, the levels of histamine, total IgE, IgG1, beta-lactoglobulin specific IgE and proinflammatory factors IL-4 and IL-17A in serum are reduced, the levels of IFN-gamma and IL-10 are improved, and the immune balance is recovered; (3) inflammation of lung and jejunum tissues is relieved; (4) improving the oxidative stress state; (5) the intestinal barrier function is enhanced; and (6) recovering the diversity of intestinal flora, and adjusting the relative abundance of key bacteria genus such as Alloprevotella and the like.

The invention relates to the technical field of medical instrument simulation and testing, in particular to a high-precision 3D printing personalized alimentary canal entity model method which comprises the steps that alimentary canal CT image data covering the range from the nasal cavity to the jejunum of a target individual and biomechanical property reference data of different sections of the alimentary canal are obtained; generating a stiffness distribution diagram; transparent flexible photosensitive resin is used as a base material, and various transparent flexible photosensitive resin materials with different elastic moduli are prepared by adding a modifier; generating a printing instruction; a digital light processing 3D printer is adopted, a solid model is printed layer by layer according to a printing instruction, the distributed transparent flexible photosensitive resin material is automatically switched and used in the printing process, and exposure parameters of ultraviolet light are dynamically adjusted based on the characteristics of the currently used transparent flexible photosensitive resin material so as to control the post-curing elastic modulus of different sections. Through accurate elastic modulus control and dynamic exposure adjustment, the mechanical properties of different sections of the digestive tract are highly consistent with the actual situation.

The invention discloses a rapeseed-derived polypeptide capable of relieving injury of vomitoxin to piglet intestines and application of the rapeseed-derived polypeptide. The rapeseed polypeptide provided by the invention is prepared from the following peptide fragments: YLKDPSKF, IVGI, KGFF, GHQYIVT, YYF, FHLY, STFSEY, SVDKQIAK, FYSI and ALRAPLGQN. The rapeseed polypeptide has the advantages that the rapeseed polypeptide is prepared from the following peptide fragments; the method comprises the following steps: extracting rapeseed protein isolate from fat rapeseed dregs, and carrying out enzymolysis, dialysis and ultrafiltration to obtain the micromolecular rapeseed polypeptide with the molecular weight of less than 1kDa. Researches show that the polypeptide can relieve piglet weight loss caused by vomitoxin intestinal injury, can reduce the concentration of proinflammatory factors in serum, and can relieve inflammatory factor infiltration; meanwhile, the rapeseed polypeptide can protect the jejunum tissue of the piglet and reverse jejunum tissue crypt hypertrophy and intestinal villus shortening caused by a DON model group, has an intestinal tissue injury recovery effect, and can be applied to deep processing of rapeseed meal and relieving vomitoxin-induced piglet intestinal injury.

The utility model discloses a mould is placed under the mould of simulation ultrasonic real -time guidance of nasal -intestinal tube, including the digestive tract simulation body of simulation esophagus, stomach, duodenum and jejunum, it adopts the flexible material of ultrasonic wave penetration, front surface is not transparent, back surface is transparent, and its inside esophagus is used for filling air, and the part except esophagus is used for filling liquid, the digestive tract simulation body is fixed between two organic glass, front surface is not transparent, back surface is transparent, the organic glass of front surface opens at esophagus place, antrum gastricum place respectively, as the acoustic window of ultrasonic, is used for the inflation sign of esophagus and antrum gastricum nasal -intestinal tube catheter tip cloud sign of ultrasonic observation when placing nasal -intestinal tube respectively, is provided with many resistance positions at stomach and duodenum, for making nasal -intestinal tube catheter tip forward into the obstruction. The utility model can simulate the sign of ultrasonic under different conditions on the mould, effectively simulates the actual entering process of nasal -intestinal tube under the real -time guidance of ultrasonic, and facilitates simulation teaching demonstration.

A duodenum built-in jejunum cannula releasing system and a use method therefor, the system including: implanting device, pushing assembly, fixing line and releasing line. The implanting device includes storage pipe shell, releaser, membrane pipe and stent; the storage pipe shell is tubular and provided with near-end opening and far-end opening; the membrane pipe and the stent which are to be released are arranged in the storage pipe shell in folded state; the pushing assembly includes push-pull guiding wire, inner pipe, middle pipe and outer pipe which are sleeved with one another from the inside out in sequence and may move relative to one another.

The application discloses a nasal jejunum catheterization robot and a system thereof, and relates to the technical field of medical devices.The robot control box is connected with a nasal jejunum tube at the output end, and one end of the nasal jejunum tube is provided with a robot end part.The inside of the robot end part is provided with a steering assembly, and one end of the steering assembly is mounted with an electromagnetic chuck.The one end of the electromagnetic chuck is connected with one side of the tubular device through magnetic attraction.The input end of the electromagnetic chuck is electrically connected with a wire harness.The one end of the wire harness penetrates through the inside of the nasal jejunum tube and is connected with the inside control end of the robot control box.The robot end part comprises a leather sheath, and a plurality of holes are formed in the outer wall of the leather sheath.A plurality of sensors are mounted in the holes respectively, and the sensors are electrically connected with the wire harness respectively.

This invention discloses a fermented microbial preparation for inhibiting Campylobacter jejuni in calves, its preparation method, and its application. The fermented microbial preparation is a co-fermentation product of a mixture of Lygodium japonicum decoction and liquid culture medium, inoculated with Lactobacillus paracasei; the Lactobacillus paracasei is... Lacticaseibacillus paracasei KQNIU. Comparative experiments in this invention revealed that, compared to other types of traditional Chinese medicine, the inhibitory effect of fermented Lygodium japonicum on Campylobacter jejuni is significantly higher than that of other types of traditional Chinese medicine. Furthermore, the co-fermentation of the traditional Chinese medicine Lygodium japonicum and the probiotic Lactobacillus paracasei KQNIU achieves a synergistic effect between the two, overcoming the problem of limited efficacy when used alone.

1. Name of the product in this design: Anastomosis device (inserted fully endocavitary electrically powered tubular type - straight). 2. Application of this design: for laparoscopic anastomosis of esophagus and jejunum, intestine to intestine, or thoracoscopic anastomosis of esophagus and jejunum or esophagus and stomach within the thoracic cavity. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D view 1. 5. Other situations requiring explanation: Part A is made of transparent material.

The utility model provides an improved jejunum fistulization tube, and relates to the technical field of fistulization tubes, the improved jejunum fistulization tube comprises a fistulization tube body, the fistulization tube body comprises a tube body used for feeding, the tube body comprises a feeding section and a discharging hole, and the feeding section is arranged at one end of the tube body; the discharging hole is formed in the side face, away from the feeding section, of the pipe body, so that the nutrient solution can flow in from the feeding section and flow out from the discharging hole; the discharge hole is in a long strip shape along the axis of the pipe body; the long-strip-shaped discharging holes are formed in the end, extending into the jejunum, of the tube body, so that a nutrient solution in the tube body flows into the jejunum from the discharging holes, when particles in the nutrient solution block the discharging holes, the discharging holes have enough space so that the particles can flow into the jejunum from the discharging holes in different positions, and the blocking risk is reduced.

1. The name of the product of this design: Esophageal jejunal stent (bristle type). 2. Purpose of the product of this design: to prevent or treat anastomotic fistula after reconstruction and anastomosis of the digestive tract in the human body. 3. The key points of the design of this product are its shape and its combination of elements. 4. The picture or photo that best illustrates the key points of the design: main view.

The application discloses application of Fagopyrum cymosum root powder in preparation of products for improving egg quality or intestinal health of laying ducks, and belongs to the technical field of feed additives. The application provides application of Fagopyrum cymosum root powder in preparation of products for improving egg quality or intestinal health of laying ducks, and the Fagopyrum cymosum root powder can improve eggshell strength, yolk color and eggshell proportion, improve the content of aspartic acid, threonine, glutamic acid, glycine, cystine and leucine in yolk, improve the content of arachidonic acid and arachidonic acid in yolk, and reduce the content of saturated fatty acid in yolk; the Fagopyrum cymosum root powder can improve the activity of maltase in the mucous membrane of the jejunum of laying ducks, and can improve the diversity and abundance of intestinal flora of laying ducks. The application provides a new strategy for improving egg quality or intestinal health of laying ducks.

The application discloses a compound plant essential oil preparation for improving intestinal tract barrier function of laying hens and a preparation method thereof, relates to the technical field of livestock and poultry intestinal tract health regulation, and comprises microcapsule particles with a multilayer structure, which sequentially comprises a core layer, a first coating layer, an intermediate functional layer and a second coating layer; the compound plant essential oil preparation for improving intestinal tract barrier function of laying hens and the preparation method thereof are characterized in that the preparation is arranged as a multilayer microcapsule structure composed of the core layer, the first coating layer, the intermediate functional layer and the second coating layer in sequence, and the first coating layer and the second coating layer adopt enteric polymers with different pH dissolution ranges, so that the intermediate functional layer can be released first in the pH environment from the duodenum to the jejunum, and the core layer is released subsequently in the pH environment of the ileum, thereby realizing the graded and zoned release of active components of plant essential oils in the front and rear sections of the small intestine of laying hens, and matching different essential oil components with the barrier repair requirements of each section of the intestinal tract.

The invention discloses a campylobacter jejuni rapid detection method and a kit based on a specific molecular target rpoZ. The sequence of the molecular target is as shown in SEQ ID NO. 1. The PCR / qPCR detection kit for detecting campylobacter jejuni is established on the basis of the target rpoZ, has extremely high specificity, only has specific amplification signals for nucleic acid of campylobacter jejuni, and has no cross reaction for other common food-borne pathogenic bacteria such as escherichia coli, salmonella and staphylococcus aureus. Meanwhile, the sensitivity is high, and the nucleic acid detection target can detect the campylobacter jejuni genome DNA as low as 132fg / mu L. The method can effectively detect campylobacter jejuni in samples such as food, is high in specificity, good in accuracy and simple to operate, and can be applied to the fields of food safety and the like.

The utility model discloses a multifunctional bedside nasojejunum nutrition tube placement training device which comprises a head and neck bionic block, a nasopharyngeal cavity channel is arranged in the head and neck bionic block; the pleuroperitoneal cavity frame comprises a hard simulation frame and bionic skin covering the hard simulation frame. Wherein the head and neck bionic block and the pleuroperitoneal cavity frame are integrally formed or detachably connected. A bionic esophagus, a bionic stomach, a bionic duodenum and a part of bionic jejunum which are sequentially connected are arranged in the pleuroperitoneal cavity frame, and a bionic cardia and a bionic pylorus are arranged at the head end and the tail end of the bionic stomach respectively. A bionic inferior vena cava, a bionic abdominal aorta, a bionic mesenteric superior vein and a bionic mesenteric superior artery are further arranged in the pleuroperitoneal cavity frame; the bionic inferior vena cava and the bionic abdominal aorta are arranged on the rear sides of the bionic stomach and the bionic duodenum, the bottom ends are closed, and the head ends penetrate out of the bionic skin; the bottom ends of the bionic superior mesenteric vein and the bionic superior mesenteric artery are closed, and the head ends of the bionic superior mesenteric vein and the bionic superior mesenteric artery partially wrap the bionic duodenum and then penetrate out of the bionic skin.

The invention relates to the technical field of microorganisms, in particular to giant spheroidin Escherichia and application of the giant spheroidin Escherichia in promoting intestinal development of piglets. The Megasphaera elsdenii provided by the invention is preserved in Guangdong Microbial Culture Collection Center on May 22, 2025, the preservation number is GDMCC No: 66369, and the Megasphaera elsdenii can be used for promoting the development of intestinal tracts and intestinal mucosa of piglets and retarding growth retardation caused by weaning stress. In addition, the in-vitro pure culture supernatant of the giant spheroidin can also improve the viability of the jejunum stem cells and promote the proliferation of the epithelial cells of the pig intestinal tract.

The invention discloses an auxiliary device for jejunostomy nursing. The auxiliary device comprises an embedded air bag, a transition section and an outer side fixing disc. One end of the transition section is fixedly connected with the embedded air bag, and the other end of the transition section is fixedly connected with the outer side fixing disc; the embedded air bag is of an internally hollow inflatable air bag structure; the device can be embedded into the stoma of a patient to communicate the intestinal tract with the outside, the device can be expanded by adding gas or liquid so as to firmly fix the device and open the enteric cavity at the same time, and the device can also lift the opening of the stoma and turn intestinal contents, so that the nursing operation of the stoma is facilitated; and an ostomy bag can be better adhered, so that the skin of a patient is protected, and the risks of intestinal fistula and anastomotic fistula are reduced.

The invention relates to the technical field of microorganisms, in particular to a lactobacillus plantarum strain LS2 and application thereof.The lactobacillus plantarum strain LS2 is separated from cheese, has the high GABA production capacity and the nitrite degradation capacity, has the antibacterial effect on enteric pathogenic bacteria including escherichia coli, salmonella enteritidis and campylobacter jejuni, and has the advantages that the lactobacillus plantarum strain LS2 is high in GABA production capacity and nitrite degradation capacity; the strain has good antibacterial function, good acid resistance and cholate resistance, and can well survive in artificial intestinal juice and artificial gastric juice; in order to improve the yield of GABA, the accelerant capable of remarkably promoting the growth of the strain LS2 is obtained by researching the accelerant, the highest survival rate of the freeze-dried powder prepared by optimizing the accelerant and the freeze-dried powder can reach 99% or above, the nitrite degradation rate can reach 98% or above, the yield of GABA is improved to 3.3 g / L or above, and the freeze-dried powder is bacterial powder capable of being widely applied.

An antimicrobial peptide having an amino acid sequence selected from SEQ ID NOs: 2-12 is provided. The antimicrobial peptide has antimicrobial activity against Gram-negative bacteria, Campylobacter jejuni, Escherichia coli, Salmonella enterica subsp. enterica serovar typhimurium, Salmonella enterica subsp. enterica serovar choleraesuis, Klebsiella pneumonica, and / or Enterobacter cloacae. A composition, pharmaceutical composition, food additive, cosmetic composition, or hygine product having an antimicrobial peptide including an amino acid sequence selected from SEQ ID NOs: 2-12 is also provided. A method of treating an infectious disease caused by bacteria including a step of administering a pharmaceutical composition having the antimicrobial peptide as an active ingredient is also provided.

The invention discloses a dual digital PCR (polymerase chain reaction) detection method for monkey campylobacter jejuni and monkey enterocolitis yersinia, and relates to the technical field of microbiological detection, the detection method comprises the following specific steps: multi-source heterogeneous data collection: collecting monkey feces and intestinal tissue samples, extracting genome DNA (deoxyribonucleic acid) by using a phenol-chloroform method, detecting the concentration and purity of DNA, and detecting the concentration and purity of the DNA; after reaching the standard, storing at-20 DEG C for later use; according to the method, high-sensitivity and specific detection on the two pathogenic bacteria is realized through a combination of well-designed primers and probes, according to the method, a gyrA gene conserved region of monkey campylobacter jejuni CJ and a POL gene conserved region of monkey yersinia enterocolitica YE are used as detection targets, and specific fragments of 249bp and 182bp are amplified respectively; the method effectively avoids interference of non-specific amplification, can simultaneously detect two pathogenic bacteria in a single reaction system through a dual digital PCR technology, accurately calculates target gene copy number concentration through a Poisson distribution formula, and improves detection accuracy and reliability.