102 results about "Jejunum" patented technology

The invention belongs to the technical field of microbial genetic engineering, and particularly relates to an alpha-2, 3-sialyltransferase mutant and application thereof. The a-2, 3-sialyltransferase mutants V175N, P198A, G173D, D174Q, F196K and A315H are obtained by performing site-specific mutagenesis on six amino acids in an a-2, 3-sialyltransferase amino acid sequence coded by a gene derived from Campylobacter jejuni (OH4384), the yield of 3 '-sialyllactose is remarkably increased by single-point mutagenesis of the V175N, the yield of 3'-sialyllactose is remarkably increased by single-point mutagenesis of the V175N, the yield of 3 '-sialyllactose is remarkably increased by single-point mutagenesis of the P198A, the G173D, the D174Q, the F196K and the A315H, the optimal recombinant microbial strain 3 '-SL-09 (V175N / P198A / D174Q) is obtained through combination of multiple mutation sites, the yield can reach 5.91 g / L, the bottleneck problem of low catalytic efficiency of sialyltransferase is effectively solved, and an enzyme element with excellent performance is provided for high-efficiency and low-cost biological manufacturing of 3'-SL.

The invention relates to the technical field of guiding devices, in particular to a nasal jejunum nutrition tube imbedding guiding device which is used for guiding the process of imbedding a flexible nasal jejunum tube into a human body. The image acquisition device acquires a picture in the direction of the advancing direction of the insertion end of the nasal jejunum tube; the bag body is arranged in a manner of extending close to the insertion end of the nasal jejunum tube; during use, the bag body does not generate binding force on the nasal jejunum tube in a low-expansion state, the shape of the nasal jejunum tube is not limited by the bag body, when the bag body is in a high-expansion state, the bag body generates binding force on the nasal jejunum tube to enable the nasal jejunum tube to be bent, and the image acquisition device comprises a camera arranged in the insertion end of the nasal jejunum tube. The insertion end of the nasointestinal tube is provided with a closing part for preventing the camera from falling off, and the problem that the throat and the pylorus cannot pass through the nasointestinal tube quickly and smoothly in the existing nasointestinal tube indwelling process can be solved by improving the existing endoscope guiding technology.

The invention relates to the technical field of microorganisms, in particular to a lactobacillus plantarum strain LM2 and application of the lactobacillus plantarum strain LM2. The strain has relatively high GABA (gamma-aminobutyric acid) production capacity and nitrite degradation capacity, has a bacteriostatic effect on intestinal pathogens such as staphylococcus aureus, salmonella enteritidis, listeria monocytogenes and campylobacter jejuni, has a good bacteriostatic function, also has good acid resistance and cholate resistance, and can be used for preparing a bacterial strain for treating intestinal diseases. The strain can well survive in artificial intestinal juice and artificial gastric juice; in order to increase the yield of GABA, the accelerant capable of remarkably promoting the growth of the strain LM2 is obtained by researching the accelerant, and the highest survival rate of the freeze-dried powder prepared by optimizing the accelerant and the freeze-dried powder can reach 99% or above, the nitrite degradation rate can reach 99% or above, the yield of GABA is increased to 3.0 g / L or above, and the freeze-dried powder is bacterial powder capable of being widely applied.

The invention belongs to the technical field of biological medicines, and particularly relates to application of UH15-38 in preparation of a medicine for preventing and treating PEDV-PoRVA co-infection. According to the invention, a PEDV and PoRVA co-infected piglet model is systematically established for the first time, a high-throughput transcriptomics means is combined, an enhancement mechanism of co-infection on host pathogenicity is deeply analyzed from an immune regulation level, and based on the mechanism, UH15-38 is found to be capable of effectively treating PEDV and PoRVA co-infected piglets. Experimental data show that the UH15-38 remarkably relieves clinical diarrhea symptoms caused by co-infection, reduces the copy number of PoRVA and PEDV viruses, effectively inhibits duplication of the viruses in intestinal tracts, remarkably reduces the expression levels of IL-1beta and p-MLKL in jejunum tissues, and reduces necrotic apoptosis and inflammatory response. The UH15-38 can provide a new application value for the treatment of the porcine viral diarrhea.

The invention discloses a probiotic biofilm pulse ultrasonic atomization spraying device, and particularly relates to the technical field of jejunum nutrient canal processing.The device comprises a base, and a spraying sleeve shell and an electric frame are installed at the top of the base; an inner conversion frame is rotationally arranged in the spraying sleeve shell, a linear placement groove is formed in the outer side of the inner conversion frame, and multiple sets of vacuum fixing clamps are slidably connected to the inner walls of the two sides of the linear placement groove; a rotary drawing mechanism is arranged in the electrical frame and comprises a fixed feeding pipe, a drawing sleeve and an air supply shaft seat; the drawing sleeve penetrates through the middle of the air supply shaft seat and is in threaded connection with the air supply shaft seat, the air supply shaft seat is provided with a second cooling pipeline and an air outlet ring cavity from inside to outside, a first communicating hose is connected between the second cooling pipeline and the fixed feeding pipe, a vortex sheet is arranged in the air outlet ring cavity, and the air outlet ring cavity can be filled with inert gas; and piezoelectric ultrasonic atomization sheets are mounted in the end parts of the cooling pipeline II and the drawing sleeve. Environmental microbial pollution is effectively prevented, and the spraying effect of the probiotic biological membrane is guaranteed.

The invention discloses application of bifidobacterium longum subsp. Infantis CCFM1269 in preparation of a product for relieving lactoglobulin allergy, and belongs to the technical field of microorganisms. The bifidobacterium longum subsp. Infantis CCFM1269 provided by the invention can relieve food allergy, and the food allergy can be relieved specifically as follows: (1) allergy symptom score and diarrhea occurrence rate can be relieved, and anus temperature and immune organ index can be recovered; (2) the immune response is regulated, the levels of histamine, total IgE, IgG1, beta-lactoglobulin specific IgE and proinflammatory factors IL-4 and IL-17A in serum are reduced, the levels of IFN-gamma and IL-10 are improved, and the immune balance is recovered; (3) inflammation of lung and jejunum tissues is relieved; (4) improving the oxidative stress state; (5) the intestinal barrier function is enhanced; and (6) recovering the diversity of intestinal flora, and adjusting the relative abundance of key bacteria genus such as Alloprevotella and the like.

The invention relates to the technical field of medical instrument simulation and testing, in particular to a high-precision 3D printing personalized alimentary canal entity model method which comprises the steps that alimentary canal CT image data covering the range from the nasal cavity to the jejunum of a target individual and biomechanical property reference data of different sections of the alimentary canal are obtained; generating a stiffness distribution diagram; transparent flexible photosensitive resin is used as a base material, and various transparent flexible photosensitive resin materials with different elastic moduli are prepared by adding a modifier; generating a printing instruction; a digital light processing 3D printer is adopted, a solid model is printed layer by layer according to a printing instruction, the distributed transparent flexible photosensitive resin material is automatically switched and used in the printing process, and exposure parameters of ultraviolet light are dynamically adjusted based on the characteristics of the currently used transparent flexible photosensitive resin material so as to control the post-curing elastic modulus of different sections. Through accurate elastic modulus control and dynamic exposure adjustment, the mechanical properties of different sections of the digestive tract are highly consistent with the actual situation.

The invention discloses a rapeseed-derived polypeptide capable of relieving injury of vomitoxin to piglet intestines and application of the rapeseed-derived polypeptide. The rapeseed polypeptide provided by the invention is prepared from the following peptide fragments: YLKDPSKF, IVGI, KGFF, GHQYIVT, YYF, FHLY, STFSEY, SVDKQIAK, FYSI and ALRAPLGQN. The rapeseed polypeptide has the advantages that the rapeseed polypeptide is prepared from the following peptide fragments; the method comprises the following steps: extracting rapeseed protein isolate from fat rapeseed dregs, and carrying out enzymolysis, dialysis and ultrafiltration to obtain the micromolecular rapeseed polypeptide with the molecular weight of less than 1kDa. Researches show that the polypeptide can relieve piglet weight loss caused by vomitoxin intestinal injury, can reduce the concentration of proinflammatory factors in serum, and can relieve inflammatory factor infiltration; meanwhile, the rapeseed polypeptide can protect the jejunum tissue of the piglet and reverse jejunum tissue crypt hypertrophy and intestinal villus shortening caused by a DON model group, has an intestinal tissue injury recovery effect, and can be applied to deep processing of rapeseed meal and relieving vomitoxin-induced piglet intestinal injury.

The utility model discloses a mould is placed under the mould of simulation ultrasonic real -time guidance of nasal -intestinal tube, including the digestive tract simulation body of simulation esophagus, stomach, duodenum and jejunum, it adopts the flexible material of ultrasonic wave penetration, front surface is not transparent, back surface is transparent, and its inside esophagus is used for filling air, and the part except esophagus is used for filling liquid, the digestive tract simulation body is fixed between two organic glass, front surface is not transparent, back surface is transparent, the organic glass of front surface opens at esophagus place, antrum gastricum place respectively, as the acoustic window of ultrasonic, is used for the inflation sign of esophagus and antrum gastricum nasal -intestinal tube catheter tip cloud sign of ultrasonic observation when placing nasal -intestinal tube respectively, is provided with many resistance positions at stomach and duodenum, for making nasal -intestinal tube catheter tip forward into the obstruction. The utility model can simulate the sign of ultrasonic under different conditions on the mould, effectively simulates the actual entering process of nasal -intestinal tube under the real -time guidance of ultrasonic, and facilitates simulation teaching demonstration.

The application discloses a nasal jejunum catheterization robot and a system thereof, and relates to the technical field of medical devices.The robot control box is connected with a nasal jejunum tube at the output end, and one end of the nasal jejunum tube is provided with a robot end part.The inside of the robot end part is provided with a steering assembly, and one end of the steering assembly is mounted with an electromagnetic chuck.The one end of the electromagnetic chuck is connected with one side of the tubular device through magnetic attraction.The input end of the electromagnetic chuck is electrically connected with a wire harness.The one end of the wire harness penetrates through the inside of the nasal jejunum tube and is connected with the inside control end of the robot control box.The robot end part comprises a leather sheath, and a plurality of holes are formed in the outer wall of the leather sheath.A plurality of sensors are mounted in the holes respectively, and the sensors are electrically connected with the wire harness respectively.

This invention discloses a fermented microbial preparation for inhibiting Campylobacter jejuni in calves, its preparation method, and its application. The fermented microbial preparation is a co-fermentation product of a mixture of Lygodium japonicum decoction and liquid culture medium, inoculated with Lactobacillus paracasei; the Lactobacillus paracasei is... Lacticaseibacillus paracasei KQNIU. Comparative experiments in this invention revealed that, compared to other types of traditional Chinese medicine, the inhibitory effect of fermented Lygodium japonicum on Campylobacter jejuni is significantly higher than that of other types of traditional Chinese medicine. Furthermore, the co-fermentation of the traditional Chinese medicine Lygodium japonicum and the probiotic Lactobacillus paracasei KQNIU achieves a synergistic effect between the two, overcoming the problem of limited efficacy when used alone.

1. Name of the product in this design: Anastomosis device (inserted fully endocavitary electrically powered tubular type - straight). 2. Application of this design: for laparoscopic anastomosis of esophagus and jejunum, intestine to intestine, or thoracoscopic anastomosis of esophagus and jejunum or esophagus and stomach within the thoracic cavity. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D view 1. 5. Other situations requiring explanation: Part A is made of transparent material.

The utility model provides an improved jejunum fistulization tube, and relates to the technical field of fistulization tubes, the improved jejunum fistulization tube comprises a fistulization tube body, the fistulization tube body comprises a tube body used for feeding, the tube body comprises a feeding section and a discharging hole, and the feeding section is arranged at one end of the tube body; the discharging hole is formed in the side face, away from the feeding section, of the pipe body, so that the nutrient solution can flow in from the feeding section and flow out from the discharging hole; the discharge hole is in a long strip shape along the axis of the pipe body; the long-strip-shaped discharging holes are formed in the end, extending into the jejunum, of the tube body, so that a nutrient solution in the tube body flows into the jejunum from the discharging holes, when particles in the nutrient solution block the discharging holes, the discharging holes have enough space so that the particles can flow into the jejunum from the discharging holes in different positions, and the blocking risk is reduced.

The application discloses application of Fagopyrum cymosum root powder in preparation of products for improving egg quality or intestinal health of laying ducks, and belongs to the technical field of feed additives. The application provides application of Fagopyrum cymosum root powder in preparation of products for improving egg quality or intestinal health of laying ducks, and the Fagopyrum cymosum root powder can improve eggshell strength, yolk color and eggshell proportion, improve the content of aspartic acid, threonine, glutamic acid, glycine, cystine and leucine in yolk, improve the content of arachidonic acid and arachidonic acid in yolk, and reduce the content of saturated fatty acid in yolk; the Fagopyrum cymosum root powder can improve the activity of maltase in the mucous membrane of the jejunum of laying ducks, and can improve the diversity and abundance of intestinal flora of laying ducks. The application provides a new strategy for improving egg quality or intestinal health of laying ducks.

The application discloses a compound plant essential oil preparation for improving intestinal tract barrier function of laying hens and a preparation method thereof, relates to the technical field of livestock and poultry intestinal tract health regulation, and comprises microcapsule particles with a multilayer structure, which sequentially comprises a core layer, a first coating layer, an intermediate functional layer and a second coating layer; the compound plant essential oil preparation for improving intestinal tract barrier function of laying hens and the preparation method thereof are characterized in that the preparation is arranged as a multilayer microcapsule structure composed of the core layer, the first coating layer, the intermediate functional layer and the second coating layer in sequence, and the first coating layer and the second coating layer adopt enteric polymers with different pH dissolution ranges, so that the intermediate functional layer can be released first in the pH environment from the duodenum to the jejunum, and the core layer is released subsequently in the pH environment of the ileum, thereby realizing the graded and zoned release of active components of plant essential oils in the front and rear sections of the small intestine of laying hens, and matching different essential oil components with the barrier repair requirements of each section of the intestinal tract.

The utility model discloses a multifunctional bedside nasojejunum nutrition tube placement training device which comprises a head and neck bionic block, a nasopharyngeal cavity channel is arranged in the head and neck bionic block; the pleuroperitoneal cavity frame comprises a hard simulation frame and bionic skin covering the hard simulation frame. Wherein the head and neck bionic block and the pleuroperitoneal cavity frame are integrally formed or detachably connected. A bionic esophagus, a bionic stomach, a bionic duodenum and a part of bionic jejunum which are sequentially connected are arranged in the pleuroperitoneal cavity frame, and a bionic cardia and a bionic pylorus are arranged at the head end and the tail end of the bionic stomach respectively. A bionic inferior vena cava, a bionic abdominal aorta, a bionic mesenteric superior vein and a bionic mesenteric superior artery are further arranged in the pleuroperitoneal cavity frame; the bionic inferior vena cava and the bionic abdominal aorta are arranged on the rear sides of the bionic stomach and the bionic duodenum, the bottom ends are closed, and the head ends penetrate out of the bionic skin; the bottom ends of the bionic superior mesenteric vein and the bionic superior mesenteric artery are closed, and the head ends of the bionic superior mesenteric vein and the bionic superior mesenteric artery partially wrap the bionic duodenum and then penetrate out of the bionic skin.

The invention discloses an auxiliary device for jejunostomy nursing. The auxiliary device comprises an embedded air bag, a transition section and an outer side fixing disc. One end of the transition section is fixedly connected with the embedded air bag, and the other end of the transition section is fixedly connected with the outer side fixing disc; the embedded air bag is of an internally hollow inflatable air bag structure; the device can be embedded into the stoma of a patient to communicate the intestinal tract with the outside, the device can be expanded by adding gas or liquid so as to firmly fix the device and open the enteric cavity at the same time, and the device can also lift the opening of the stoma and turn intestinal contents, so that the nursing operation of the stoma is facilitated; and an ostomy bag can be better adhered, so that the skin of a patient is protected, and the risks of intestinal fistula and anastomotic fistula are reduced.

The invention relates to the technical field of microorganisms, in particular to a lactobacillus plantarum strain LS2 and application thereof.The lactobacillus plantarum strain LS2 is separated from cheese, has the high GABA production capacity and the nitrite degradation capacity, has the antibacterial effect on enteric pathogenic bacteria including escherichia coli, salmonella enteritidis and campylobacter jejuni, and has the advantages that the lactobacillus plantarum strain LS2 is high in GABA production capacity and nitrite degradation capacity; the strain has good antibacterial function, good acid resistance and cholate resistance, and can well survive in artificial intestinal juice and artificial gastric juice; in order to improve the yield of GABA, the accelerant capable of remarkably promoting the growth of the strain LS2 is obtained by researching the accelerant, the highest survival rate of the freeze-dried powder prepared by optimizing the accelerant and the freeze-dried powder can reach 99% or above, the nitrite degradation rate can reach 98% or above, the yield of GABA is improved to 3.3 g / L or above, and the freeze-dried powder is bacterial powder capable of being widely applied.

An antimicrobial peptide having an amino acid sequence selected from SEQ ID NOs: 2-12 is provided. The antimicrobial peptide has antimicrobial activity against Gram-negative bacteria, Campylobacter jejuni, Escherichia coli, Salmonella enterica subsp. enterica serovar typhimurium, Salmonella enterica subsp. enterica serovar choleraesuis, Klebsiella pneumonica, and / or Enterobacter cloacae. A composition, pharmaceutical composition, food additive, cosmetic composition, or hygine product having an antimicrobial peptide including an amino acid sequence selected from SEQ ID NOs: 2-12 is also provided. A method of treating an infectious disease caused by bacteria including a step of administering a pharmaceutical composition having the antimicrobial peptide as an active ingredient is also provided.

The invention discloses a dual digital PCR (polymerase chain reaction) detection method for monkey campylobacter jejuni and monkey enterocolitis yersinia, and relates to the technical field of microbiological detection, the detection method comprises the following specific steps: multi-source heterogeneous data collection: collecting monkey feces and intestinal tissue samples, extracting genome DNA (deoxyribonucleic acid) by using a phenol-chloroform method, detecting the concentration and purity of DNA, and detecting the concentration and purity of the DNA; after reaching the standard, storing at-20 DEG C for later use; according to the method, high-sensitivity and specific detection on the two pathogenic bacteria is realized through a combination of well-designed primers and probes, according to the method, a gyrA gene conserved region of monkey campylobacter jejuni CJ and a POL gene conserved region of monkey yersinia enterocolitica YE are used as detection targets, and specific fragments of 249bp and 182bp are amplified respectively; the method effectively avoids interference of non-specific amplification, can simultaneously detect two pathogenic bacteria in a single reaction system through a dual digital PCR technology, accurately calculates target gene copy number concentration through a Poisson distribution formula, and improves detection accuracy and reliability.

The application provides a jejunum catheterization intelligent navigation method and system based on real-time ultrasonic contrast, relates to the technical field of biological medical engineering, and comprises the following steps: collecting multiple data sets; performing unified time base alignment on the multiple data sets, and performing structure encapsulation and normalization processing to obtain jejunum catheterization navigation acquisition data frames; performing harmonic selective extraction on the contrast frames in the jejunum catheterization navigation acquisition data frames to obtain a harmonic selective enhancement result set; generating a tube body probability graph based on the harmonic selective enhancement result set to obtain a tube body candidate data set; constructing a candidate tube body space-time graph to generate a consistent tube body trajectory set; performing head positioning judgment and reliability evaluation on the consistent tube body trajectory set to determine a jejunum catheterization head positioning result; generating navigation prompt information based on the jejunum catheterization head positioning result, and outputting a navigation interaction result; performing navigation comprehensive risk evaluation on the navigation interaction result, and triggering a closed-loop self-adaption and degradation strategy according to the evaluation result.

One or more antibodies, particularly nanobodies or VHH single domain antibodies, directed against one or more Campylobacter bacteria targets with roles in Campylobacter colonization, particularly Campylobacter jejuni, in animals, particularly in poultry are provided. The nanobodies are useful in reducing or blocking Campylobacter colonization or infection. The invention provides methods for reducing or blocking Campylobacter colonization or infection, for improving food safety, and for reducing bacterial gastroenteritis in animals, including humans.

The invention belongs to the field of preparation of natural products related to health, and particularly discloses a composition for promoting health of heart and cerebral vessels as well as a preparation method and application of the composition. The composition is prepared from the following raw materials in parts by mass: 10 to 30 parts of earthworm protein, 5 to 20 parts of milk tripeptide, 1 to 10 parts of rice bran fatty alkanol, 5 to 25 parts of aronia melanocarpa extract, 3 to 15 parts of mulberry leaf extract and 1 to 8 parts of micro-embedded lactobacillus plantarum, the composition is prepared into an enteric-coated preparation by a split-phase granulation process: an earthworm protein / lactotripeptide / alkanol coating is an enteric-coated pellet I, an aronia melanocarpa / mulberry leaf extract coating is a sustained-release pellet II, and the enteric-coated pellet I and the sustained-release pellet II are mixed with probiotic pellets and filled into enteric-coated capsules. The system releases protein peptides in duodenum, releases plant extracts slowly in jejunum and releases probiotics in colon, so that the synergistic effects of reducing blood sugar and regulating lipid, resisting atherosclerosis and improving diabetic vasculopathy are remarkably improved.

The invention discloses a pig jejunum crypt separation and organoid model construction method, and belongs to the technical field of animal tissue separation and culture. The method specifically comprises the following steps: intestinal tract collection, cleaning solution preparation, intestinal tract treatment, tissue trimming, tissue cleaning, digestive juice preparation, tissue digestion, cell mass separation, crypt separation, crypt counting, crypt collection, crypt inoculation and organoid culture. The porcine jejunum organ obtained by the method has a typical three-dimensional cystic structure, has complete epithelial polar and functional cell types, including microvillus, goblet cells, panning cells and endocrine cells, and shows good barrier function and drug metabolic activity. A reliable in-vitro model platform is provided for pig intestinal development, nutrient absorption, pathogen infection mechanism research and precision medical treatment, meanwhile, a technical foundation is laid for large-scale application of organoids, and the method has important scientific significance and clinical value.

1. The name of this design product: Four-chamber two-balloon tube. 2. Purpose of the product with this design: used for jejunal nutrition, gastric decompression, and prevention of intestinal and gastric mucosal damage. 3. The key point of the design of this product lies in its shape. 4. The picture or photo that best illustrates the key points of the design: Design 1 3D drawing. 5. Designate Design 1 as the base design.

The utility model discloses a double-interface side hole type nutrition pusher which comprises a nutrition tube, a supporting tube and a path finding guide tube, the supporting tube is movably arranged in the nutrition tube in a penetrating mode, and the path finding guide tube is movably arranged in the supporting tube in a penetrating mode. The nutrient canal is of a two-way joint structure and comprises a first sleeve joint formed by the near end of the nutrient canal and a second sleeve joint located on one side of the first sleeve joint, and a plurality of first interlayer side holes are formed in the near end of a canal body of the nutrient canal. The nutrient canal adopts the structural design of double interfaces, double cavities and the near-end side hole, the far end of the nutrient canal is arranged in the jejunum and can be used as the nutrient canal, the farthest end of the side hole is provided with an MARK mark, and the MARK does not exceed the pylorus when the nutrient canal is arranged, so that the side hole is positioned in the gastral cavity and is used for gastrointestinal decompression, and the nutrient canal is particularly suitable for patients suffering from gastric paralysis and needing enteral nutrition.

The invention provides a polypeptide that is antigenic in a host. The invention also provides a vaccine for use in reducing or preventing Campylobacter colonization in a host. The vaccine comprises a polypeptide of the present invention and / or antibodies against the polypeptide of the present invention. The host may be a human, cow, sheep, goat, and chicken. The invention has particular application for reducing or preventing Campylobacter jejuni colonization in poultry. The invention also provides a vaccine for use in reducing or preventing campylobacteriosis; and a vaccine composition comprising the polypeptide of the present invention; or antibodies raised against the polypeptide of the present invention, in association with a pharmaceutically acceptable vehicle useful for inducing an immune response in a host.

The present application relates to the field of microbial technology, in particular to bacillus subtilis BS-1 and its application in water buffalo silage feed, the strain BS-1 of the present application has good bacteriostatic activity on escherichia coli, salmonella and streptococcus uberis, after mixing and fermenting according to a specific proportion with orange peel and cassava residue, the bacteriostatic spectrum of the obtained silage feed is significantly enhanced, which can simultaneously inhibit escherichia coli, campylobacter jejuni, salmonella, streptococcus uberis, staphylococcus aureus and streptococcus agalactiae that cause diarrhea and mastitis. In the feed, after mixing 60%-75% of orange peel and 25%-40% of cassava residue, the crude protein content of the fermentation product is significantly improved, and the fiber content is reduced. Feeding test shows that the feed can significantly improve the milk yield and milk quality of lactating water buffalo, and effectively alleviate diarrhea. The present application provides a safe, efficient and nutritious silage feed with disease prevention function.

The present application relates to the field of agricultural husbandry and veterinary technology, in particular to a protease-resistant antibacterial peptide FR and application thereof. The present application provides a protease-resistant antibacterial peptide FR, and the amino acid sequence of the antibacterial peptide FR is shown as SEQ ID NO. 1. The results of the specific implementation of the present application show that the protease-resistant antibacterial peptide FR provided by the present application has the activity of resisting gram-negative bacteria (Escherichia coli, Shigella flexneri, Salmonella and Campylobacter jejuni, etc.), has low hemolytic activity, can resist the cleavage of high-concentration protease, and has very good application prospect. In summary, the antibacterial peptide FR provided by the present application has the advantages of resisting gastrointestinal environment, resisting high salt and resisting protease cleavage, can realize the killing effect on gram-negative bacteria in the presence of enzymes and salt ions, and has the characteristics of low hemolytic activity, thereby providing a new way for the development of antibiotic substitutes.

Methods and apparatuses (e.g., devices, systems, etc.) for forming a bypass between different luminal body regions. The methods and apparatuses described herein may be used to join different portions of a patient's GI tract, including but not limited to forming a bypass to reduce nutritional update, decrease weight, and / or improve diabetes control. In some cases these methods and apparatuses may be used for performing a gastrojejunostomy bypass.