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Lactobacillus plantarum CCFM8724 and application thereof

A technology of CCFM8724 and Lactobacillus plantarum, applied in the field of microorganisms, to achieve the effect of good adhesion ability

Active Publication Date: 2012-07-04
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on lactic acid bacteria and probiotics against Campylobacter jejuni

Method used

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  • Lactobacillus plantarum CCFM8724 and application thereof
  • Lactobacillus plantarum CCFM8724 and application thereof
  • Lactobacillus plantarum CCFM8724 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0127] Embodiment 1: Analytical experiment of antibacterial substances in the fermentation supernatant of Lactobacillus plantarum CCFM8724 of the present invention

[0128] Adopt the Oxford cup method to determine the main substance of the antibacterial effect of the fermentation supernatant of Lactobacillus plantarum CCFM8724 by the following experiments:

[0129] (1) Heat treatment: heat the supernatant of Lactobacillus plantarum CCFM8724 obtained by the method described in this manual at a temperature of 100° C. for 20 minutes, and then carry out the antibacterial test.

[0130] (2) pH adjustment: the pH value of the supernatant of Lactobacillus plantarum CCFM8724 was adjusted to 6.2, and then the antibacterial experiment was carried out.

[0131] (3) Catalase treatment: After the catalase solution (1mg / ml) is filtered through a 0.22 micron membrane, it is added to the Lactobacillus plantarum according to its volume ratio of 1:10 with the supernatant of Lactobacillus planta...

Embodiment 2

[0139] Example 2: Adhesion experiment of Lactobacillus plantarum CCFM8724 on intestinal epithelial cells.

[0140] Adhesion was determined by the following test method:

[0141] (1) Intestinal epithelial cell line HT-29 cell culture

[0142] The intestinal epithelial cell line HT-29 cells (purchased from the Cell Bank of the Type Culture Collection Committee of the Chinese Academy of Sciences) were cultured using RPMI1640 medium (Gibco). HT-29 cells were placed in an incubator at 5% CO 2 The culture was carried out under the condition of atmosphere and temperature of 37°C, and the culture medium was changed once every 48 hours of culture, so that the culture continued for 15 days. Insert sterile coverslips into a 6-well cell culture plate, add 2 mL of cells to the wells at a concentration of 2 × 10 5 cells / mL of this cell culture medium, and the cell culture medium was changed every day until a monolayer of cells formed on the coverslip.

[0143] (2) Adhesion test

[0144...

Embodiment 3

[0148] Example 3: The experiment of Lactobacillus plantarum CCFM8724 inhibiting the adhesion of Campylobacter jejuni to intestinal epithelial cells HT-29.

[0149] The experimental method is as follows:

[0150] Use 0.2% trypsin solution to digest the cultured HT-29 cells and adjust the cell concentration to 2×10 5 pcs / mL -1 , and then added to a 6-well cell culture plate, 2ml per well, in RPMI1640 medium at 37°C, 5% CO 2 and 95% air for cultivation. The cell culture medium was changed every day until a monolayer of cells was formed. The monolayer cells were washed 3 times with PBS buffer (pH=7.2), and then the suspension of Lactobacillus plantarum CCFM8724 was added to each well. The MOI (multiplicity of infection) was 200:1. 2 Cultivate for 2 h under the condition of 95% air, then wash with PBS buffer (pH=7.2) for 3 times, then add Campylobacter jejuni (Campylobacter jejuni NCTC11168) bacterial suspension, MOI (multiplicity of infection) is 200:1, at temperature 37°C wi...

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Abstract

The invention relates to lactobacillus plantarum CCFM8724 and application thereof. The lactobacillus plantarum CCFM8724 has ability of suppressing growth of campylobacter jejuni in vitro, good resistance to acid and cholate and good adhesive ability to intestinal epithelial cells, and can control the campylobacter jejuni adhering to the intestinal epithelial cells so as to suppress growth of the campylobacter jejuni in chicken. The lactobacillus plantarum CCFM8724 can be used for preparing a medicinal composition for suppressing campylobacter jejuni; a fermentation agent containing the strain of lactobacillus plantarum CCFM8724 can be used for producing fermented dairy products, fermented bean products, fermented fruit / vegetable products and silage; the fermentation agent enables the products to obtain certain acidity and particular flavor; and meanwhile, the product preserving time is prolonged, and the nutritional value, digestibility and safety of the products are improved.

Description

【Technical field】 [0001] The invention belongs to the technical field of microorganisms. More specifically, the present invention relates to a kind of Lactobacillus plantarum (Lactobacillus plantarum) CCFM8724, and also relates to the application of said Lactobacillus plantarum CCFM8724. 【Background technique】 [0002] Campylobacter jejuni (Campylobacter jejuni) is a member of the Campylobacter genus and is a Gram-negative bacterium. It is a common pathogen of infectious diarrhea worldwide. The World Health Organization (WHO) listed Campylobacter jejuni disease as a common of foodborne diseases. C.jejuni is a pathogenic bacteria co-infected by humans and animals. It exists widely in the environment. C.jejuni can colonize the intestinal tract of various wild birds, poultry, and livestock, such as chickens, ducks, geese and other poultry, cats, dogs, etc. Pets, livestock such as cattle and pigs, birds such as pigeons and sparrows, and wild animals are common animal hosts. H...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/74A61P31/04A23C9/12A23C11/10A23C13/16A23C19/032A23L1/20A23L1/238A23L1/24A23L1/218C12R1/25A23L11/50A23L11/60A23L19/20A23L27/50A23L27/60A61K35/747
CPCY02A40/90
Inventor 陈卫张灏赵建新田丰伟王刚张秋香赵煜宋元达郭敏
Owner JIANGNAN UNIV
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