83 results about "Cell concentration" patented technology

The cell treatment centrifugal cup comprises a cover body and a cup body, the cover body is used for sealing an upper opening of the cup body, the cup body comprises an upper cup body and a lower cup body which are integrally formed, a circular sleeve is arranged in the centrifugal cup, the upper end of the circular sleeve is fixedly connected with the lower portion of the cover body, and the lower end of the circular sleeve extends into the lower cup body and is suspended; a plurality of stirring paddles are mounted outside the circular sleeve; the upper cup body is of a cylindrical or big-end-up circular truncated cone structure, the inner wall face of the lower cup body gradually inclines towards the center from top to bottom, at least one protrusion is arranged on the inner wall face of the lower cup body, and the protrusions are arranged in the circumferential direction of the inner wall face of the lower cup body.

The present disclosure relates to methods of determining percentage viability of a cell composition comprising cell aggregates. Such methods may include providing a first aliquot of cells and a second aliquot of cells, wherein the first aliquot of cells and the second aliquot of cells have about the same concentration of cells, measuring a total number of live cells in the first aliquot of cells; measuring a total number of cells in the second aliquot of cells, and determining the percentage viability of the cell composition as a ratio between the total number of live cells in the first aliquot of cells and the total number cells in the second aliquot of cells.

Provided are methods for the creation of ultra-high density cryopreserved cell banks. In certain embodiments, these methods employ altered perfusion culture techniques that allow for production of ultra-high density cell cultures that can be cryopreserved at unexpectedly high cell densities without the need for any cell concentration steps, while retaining excellent cell viability and quality.

Provided are a novel high-concentration cell preservation liquid and a high-concentration cell preservation method.　The cell preservation liquid is for preserving cells in a non-frozen state. The cell preservation liquid contains a basal medium and an additive added to the basal medium. The basal medium is a culture medium used for cell culture. The additive contains at least genetically modified albumin and ascorbic acid (ascorbic acid derivative). The cell preservation liquid stores the cells at a cell concentration of at least 1 × 107 cells / mL. For example, when cells are preserved in a cell preservation liquid at 4°C at a cell concentration of 1 × 108 cells / mL, the cell survival rate when 72 hours has elapsed is 80% or more.

The invention discloses a high-precision detection method of abnormal plasma cells, which comprises the following steps: preparing a film, immediately and uniformly mixing the extracted marrow fluid with an anticoagulant, sequentially diluting and centrifuging the marrow, then collecting a leukocyte layer, washing with a fluid medium RPMI 1640 / 5% FBS for at least one time, then counting the cells, adjusting the cell concentration to 1 * 10 < 6 > / ml, adding 100 [mu] l of the cell suspension into a funnel of a cell centrifugal smear device, centrifuging at room temperature for 1000 revolutions, continuing for 5 minutes, and preserving a slide filled with the cell suspension; the preparation of FISH cells comprises the following steps: firstly, preparing and pretreating a 90% formamide / 2 * SSC solution; according to the method, the accuracy and the efficiency of abnormal plasma cell detection are improved by combining an intelligent AI recognition model and a fluorescence in situ hybridization (FISH) technology. The application of the intelligent AI recognition model can reduce personal errors and improve the recognition capability of fluorescence signals, thereby improving the accuracy of detection results.

The invention provides a cell concentration device and a cell concentration control method, and belongs to the technical field of cell concentration. The cell concentration device comprises a body, at least two transducers, a container moving assembly and a control module, a containing groove is formed in the body and used for containing a cell bearing container, and a to-be-concentrated sample is stored in the cell bearing container; the two transducers are arranged on the two side faces of the containing groove respectively, the transducers are attached to the cell bearing container, and the transducers are used for converting electric signals into mechanical vibration to generate sound waves; the container moving assembly is arranged on the body and is used for lifting or lowering the cell bearing container; wherein the control module controls the two transducers to generate sound waves; meanwhile, the container moving assembly is controlled to lift the cell bearing container, so that cells in the sample are concentrated at the bottom of the cell bearing container under the action of the sound waves. The invention aims to solve the problems that the safety and integrity of concentrated cells are relatively low, the cell concentration time is long, and the cells are easy to damage.

The invention discloses a cryopreservation method and application of an NK cell cryopreservation solution, and belongs to the technical field of cell cryopreservation. The NK cell cryopreservation solution comprises DMSO, a PBS buffer solution and a hydroxyethyl starch solution; the volume fraction of the DMSO in the NK cell freezing medium is 2.8 to 3.2 percent; the cryopreservation method of the NK cell cryopreservation liquid comprises the following steps: culturing activated NK cells by using a complete culture medium added with IL-15 and IL-18, centrifuging, cleaning, preparing the NK cell cryopreservation liquid, re-suspending to a preset cell concentration by using the NK cell cryopreservation liquid, cooling, freezing, and preserving in liquid nitrogen. The NK cell cryopreservation liquid used in the invention is a universal NK cell cryopreservation liquid, and the cryopreservation method provided by the invention has a good cryopreservation effect and can be used instantly.

The utility model provides a uniform filling device for stem cell preparations, which relates to the field of stem cell preparation filling, and comprises an appliance body, a sterile bottle, a quick connection mechanism, a peristaltic pump, a filling needle, a penicillin bottle and a liquid storage bag body, and the liquid storage bag body comprises a disposable liquid storage bag; according to the liquid storage bag, the liquid storage bag body is assembled in the B + A level environment, the whole liquid storage bag is subjected to irradiation sterilization, the risk of visible foreign matter is reduced, the contamination risk is reduced to the minimum, the whole liquid storage bag is a closed pipeline, the contamination risk caused by manual operation is avoided, and the service life of the liquid storage bag is prolonged. In the filling process, the defects that each bottle of preparation finished product filled by a liquid storage bottle and a bottle shaking device is unstable in volume and large in error are overcome, after filling is finished, almost no stem cell preparation is left in the liquid storage bag, the loss of the stem cell preparation is reduced, and the filling efficiency is improved by filling through a filling machine. And the loading stability and the cell concentration uniformity of each bottle of stem cell preparation finished product are superior to those of manual loading.

This product is a work cell for cell therapy manufacturing, automating the entire manufacturing process, including cell concentration, separation, activation, gene transfer using viral vectors or electroporation, expansion culture, filling, finishing, and formulation. This product can provide a self-contained cleanroom environment with a robotic work cell, all within the size of a conference room or a small pickup truck.

The application discloses a preparation method of CCR2+ gamma delta T cells with anti-tumor activity and migration ability, and belongs to the technical field of cells; the method comprises the following steps: step (1), PBMC of peripheral blood of a healthy person is extracted, inoculated into complete culture medium, and added with zoledronic acid, IL-15 and metformin, and then is cultured in a T25 culture bottle; step (2), complete culture medium is supplemented, the cell concentration is adjusted, and then the cells are transferred into a T75 culture bottle for culture; step (3), complete culture medium is supplemented, the cell concentration is adjusted, glucocorticoid and nicotinamide are added, and then the cells are transferred into a T150 culture bottle for culture; step (4), cells are collected at D6, rinsed with normal saline, resuspended with complete culture medium, the cell concentration is adjusted, and then the cells are inoculated into a T300 culture bottle for culture; and step (5), complete culture medium is supplemented every two days from D8 to D16, the cell concentration is maintained, and then the cells are harvested at D16 to obtain CCR2+ gamma delta T cells.

A biological fluid processing device includes pump and valve systems, a controller, and an optical detection assembly. During a biological fluid processing procedure, the controller controls the operation of the pump and valve systems to separate a cell-containing fluid from the blood of a blood source and controls the optical detection assembly to emit light through the cell-containing fluid. The controller receives signals from the optical detection assembly that are indicative of the intensity of light that has passed through the cell-containing fluid. The controller receives or calculates an adjustment factor that is derived from historical data reflecting a cell collection procedure previously executed for the same blood source in which the same type of cell was collected. The controller determines an adjusted cell concentration of the cell-containing fluid based at least in part on the signals from the light detector and the adjustment factor.

A method, apparatus, and system for calculating cell concentration are disclosed. The method includes: determining an effective measurement region of a cell container, the cell container including a first encapsulation layer, a second encapsulation layer, and a solution cavity disposed between the first and second encapsulation layers; a first positioning mark is disposed on one side of the first encapsulation layer facing the solution cavity, and a second positioning mark is disposed on one side of the second encapsulation layer facing the solution cavity; dividing the effective measurement region into multiple sub-regions; focusing and acquiring an image of each sub-region; detecting the number of cells in each image and summing the number of cells in multiple images to obtain the total number of cells in the effective measurement region; focusing on the first and second positioning marks to obtain a first original cavity thickness; multiplying the first original cavity thickness by a preset adjustment coefficient to obtain an adjusted cavity thickness; calculating the volume of the effective measurement region based on the adjusted cavity thickness; and calculating the cell concentration based on the total number of cells and the volume of the effective measurement region.

The application discloses a polar microorganism separation and culture method based on a droplet microfluidic technology. The method comprises the following steps: obtaining a polar sample and performing oscillation extraction at low temperature to obtain a sample suspension; determining a plate cultivable rate R through a solid plate culture method; based on the R and a preset target number of cultivable cells N, calculating a target total number of cells T required by a droplet microfluidic experiment, and preparing a sample with a corresponding cell concentration for machine loading; generating single-cell-wrapped microdroplets through a droplet microfluidic system for the sample for machine loading, and performing long-term culture at low temperature; and screening positive droplets and purifying strains after the culture. Through the cultivable rate feedback, the sample concentration for the microfluidic loading is precisely optimized, the separation efficiency of the polar difficult-to-culture microorganism is significantly improved, the inter-species competition inhibition of the traditional plate is broken, and the diversity and novel group proportion of the obtained strains are greatly improved.

The invention provides a cell perfusion culture method and device, and the method comprises the steps: configuring a rotatable container capable of accommodating liquid, the container being provided with a first opening located in a bottom central region and a second opening located in a top central region; a cell-containing culture solution in a cell culture tank is injected into the container through the first opening for feeding; the container is controlled to rotate, and the cells are pushed to the position close to the container wall of the container through centrifugal force; liquid is continuously discharged from the second opening, so that continuous concentration of the cells is realized; the cell concentration is detected in real time, and when the cell concentration reaches a preset value, feeding, rotating and discharging operation is stopped; the conveying direction of the first opening is switched, the concentrated cells are conveyed back to the cell culture tank, perfusion culture is achieved, efficient, low-shear, low-cost and sustainable cell concentration and separation are achieved, perfusion stability and productivity are improved, and the cell concentration and separation device is suitable for large-scale cell production and industrial biopharmaceutical processes.

The invention provides novel sample chambers, units and multi-well plates, and systems and methods thereof, for built-in measurement assurance of cell counting methods and calibrated and / or quality-assured measurement and analysis of diverse types of biological cells, e.g., cell count, cell size, cell concentration, cell sub-population, cell morphology, cell viability, etc.

1. The name of the design product: fluorescent in situ imaging cell analyzer. 2. The use of the design product: the design product is used for measuring cell concentration and activity, and belongs to the field of biological automatic analysis instrument. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

A functionally closed cell processing platform designed to minimize contamination and automate cell processing. The platform integrates multiple specialized cassettes, including a reagent sample cassette (RSC) for reagent delivery and sample collection, a process fluids cassette (PFC) for waste and buffer management, and a cell processing cassette (CPC) for cell handling, along with a transfer syringe cassette (TSC) for precise fluid transfer. The system features a centrifuge capable of multi-mode operation for cell concentration and mixing, pneumatic controls for fluid and microbubble manipulation, and UV sanitizers to maintain aseptic conditions. Advanced functionalities include optical and thermal sensors for real-time monitoring, automated control of fluid and reagent handling, and a microbubble cell selection system for precise target cell isolation. This innovative platform ensures efficient, contamination-free cell processing, supporting applications in gene therapy, cell manufacturing, and biomedical research.

To provide a method of determining the concentration of one or more substances in a fluid and / or the platelet mass index of the fluid.SOLUTION: An optical detection assembly for monitoring a fluid includes a light source, a light detector array, and a controller. The controller receives signals from the light detector array that are indicative of intensity of light received by the light detector array after the light has passed through the fluid. The controller generates a scattering profile based on the signals, with the scattering profile including rising and falling edges each having a slope. The controller calculates one or both slopes and determines a platelet mass index (for a platelet-containing fluid) and / or a concentration of a substance in the fluid based on said slopes. The optical detection assembly may also be used to analyze a reference fluid having a known concentration of the substance, with signals received by the controller when analyzing the reference fluid being a factor when determining the concentration of the substance in the fluid of interest.SELECTED DRAWING: None

The invention relates to the technical field of cell extraction, in particular to a preparation method of a whole tissue single-cell suspension of human placenta villus tissue. The preparation method of the human placenta villus tissue single-cell suspension has the advantages of being small in digestive enzyme dosage, short in digestion time and high in cell activity, the prepared single-cell suspension is high in total cell concentration, the cell activity is 90% or above, the caking rate is low, the placenta villus tissue whole-tissue single-cell suspension can be rapidly prepared in a large scale, operation is easy, and the preparation method is suitable for industrial production. The method is simple in operation and low in reagent cost, can effectively retain the activity of whole tissue cells and the integrity of cell membranes, overcomes the defects of complicated operation, low cell activity and the like in the existing method, and has better application value in the fields of placenta villus tissue cell culture, organoid culture, flow cytometry, single cell sequencing and the like.

This invention discloses a method for large-scale expansion of MSC cells using 3D culture, comprising the following steps: MSC seed cell resuscitation; MSC seed cell planar culture; MSC seed cell planar expansion; MSC seed cell concentration and washing; primary scale-up culture of MSC seed cells; MSC cell washing and concentration; MSC seed cell scale-up culture; microcarrier lysis and cell suspension collection; MSC cell washing and concentration; MSC cell aliquoting and cryopreservation. The advantages of this invention are: it achieves highly efficient scale-up of MSC cells from 2D planar culture to a 3D microcarrier bioreactor, enabling cell expansion to reach more than 20-fold (primary scale-up culture) to more than 200-fold (secondary scale-up culture), while maintaining cell viability >95%.

A bioreactor system for culturing cells, comprising: —at least one bioreactor (3); and —at least one hydrocyclone (5a; 5b; 5c; 5d, 5d′; 5e) comprising: —a cell culture inlet (7) which is connected to a cell culture outlet (9) of the bioreactor (3) via a pump (11), whereby a cell culture from the bioreactor can be transferred to the hydrocyclone (5a: 5b; 5c; 5d, 5d′; 5e) when a cell culture is provided in the bioreactor, —a hydrocyclone separation part (13) into which cell culture can be introduced from the cell culture inlet (7), —a top outlet (15) through which a part of the cell culture with decreased cell concentration can be transferred after separation in the hydrocyclone separation pan (13); and —a bottom outlet (17) through which a pan of the cell culture with increased cell concentration can be transferred after separation in the hydrocyclone separation part (13), wherein said bottom outlet (17) of the hydrocyclone (5a; 5b; 5c; 5d, 5d′; 5e) is provided in a position such that when the bioreactor system is used for culturing cells the bottom outlet (17) is in connection with a headspace (21) of the bioreactor (3) and wherein said bottom outlet (17) is provided such that the bottom outlet (17) has a free space around it such that a cell culture transferred out form the bottom outlet (17) is allowed to freely discharge around the bottom outlet.

To provide an improved verification method for verifying the performance of a fluorescence-based analysis system.SOLUTION: (a) negatively staining cells in a first portion of a standard sample comprising target cells expressing a target cell marker; (b) positively staining target cells in a second portion of the standard sample; (c) passing the first and second portions of the standard sample through an instrument to obtain a fluorescence measurement indicative of target cell concentration; (d) based on the fluorescence measurement obtained in (c), each diluted sample having a nominal concentration of target cells, each nominal cell concentration being greater than the concentration of target cells indicated by the fluorescence measurement of the negatively stained first portion in (a); Preparing a dilution series comprising a plurality of diluted samples, wherein the nominal concentration in each diluted sample is different from the nominal concentration in each of the remaining diluted samples; (e) passing the dilution series of (d) through the instrument and obtaining a fluorescence measurement; and (f) comparing the nominal cell concentration of (d) and the fluorescence measurement of (e) for each diluted sample to quantify the performance of the staining method in the instrument.SELECTED DRAWING: Figure 1

The invention provides a cell concentration method and device. The device comprises a driving unit, a horizontally-arranged rotating plate, a hose penetrating through the rotating plate, a pipe body connected with the hose and a conveying assembly composed of a feeding pipe, a discharging pipe, a feeding valve and a discharging valve. And the control unit realizes continuous centrifugal concentration of liquid containing cells by coordinating the rotating speed of the driving unit and the working modes of the feeding valve and the discharging valve. The hose keeps an internal flow channel unobstructed through the anti-distortion characteristic of the hose in the rotating process, the hose body tends to be in a horizontal state along with rotation of the rotating plate to form a stable centrifugal field, and the conveying assembly achieves continuous separation and recovery of cell sediment through multi-mode flow speed regulation and control. According to the technical scheme, through the anti-twisting property of the hose and the combined structure of the horizontal pipe body, the device structure is simplified, and the manufacturing cost is reduced.

The invention relates to the technical field of waste lithium battery positive electrode material recovery, and discloses a method for recovering a ternary lithium positive electrode material through photocatalytic microbiological leaching and electrodeposition. The method specifically comprises the following steps: (1) pretreating to obtain a positive electrode material with the purity of more than or equal to 95%; (2) culturing by adopting a 9K liquid culture medium to obtain an activated bacterial solution with the thallus concentration of more than 1 * 10 < 8 > / mL (the ratio of acidithiobacillus acidophilus to thiobacillus ferrooxidans to leptospirillum is 5: 3: 2); (3) mixing a TiO2 / g-C3N4 heterojunction photocatalyst with a positive electrode material, adding an activated bacterial liquid, and carrying out synergistic leaching under the condition that the solid-to-liquid ratio is 1: 15, wherein the leaching rates of Ni, Co and Mn within 72 hours respectively reach 98.3 + / -0.5%, 96.7 + / -0.3% and 94.2 + / -0.4%; and (4) after the leachate is filtered, electro-deposition is carried out by adopting a method of adjusting electrolytic potential and pH step by step, cobalt, nickel and manganese are sequentially and selectively recovered, and lithium is recovered in the form of Li2CO3 (the purity is 99.7%). Compared with the prior art, the method has the advantages that the metal recovery rate is increased while the acid consumption and the waste water and waste gas emission are effectively reduced, and the photocatalyst and the microbial strains can be recycled.

The application discloses a method for improving the cadmium ion adsorption capacity of Escherichia coli, and belongs to the technical field of genetic engineering. E. coli BL21 to Cd 2+ , and the unit point mutation of T16C, K19C and S45C to CeMT2 can significantly improve the Cd 2+ binding capacity of CeMT2, and the cell Cd 2+ concentration of the T16C, K19C and S45C mutants is increased by 1.32 times, 1.50 times and 1.41 times, respectively. E. coli The recombinant expression of CeMT2 (K19C) and LmCadC in BL21 can obviously improve the cadmium ion adsorption capacity of Escherichia coli, and the maximum adsorption capacity reaches 8.5 mg / g.

The application discloses an impedance measurement method and device for intelligent control of an electrotransformation instrument, and relates to the technical field of impedance measurement. The type of an electrotransformation cup and the cell concentration of a sample solution are acquired to determine a switch control signal of a resistance matrix; a sine alternating current excitation of a target frequency is applied to the electrotransformation cup; the voltage on both sides of the electrotransformation cup and the voltage on both sides of the resistance matrix are detected to obtain a voltage signal; and the complex impedance of the sample solution is calculated according to the voltage signal and the resistance matrix. By acquiring the type of the electrotransformation cup and the cell concentration, the configuration of the resistance matrix is automatically controlled to realize adaptive measurement of different impedance ranges. The problems of signal saturation or weakness are effectively avoided, and the linear range and signal-to-noise ratio of measurement are improved. The complex impedance is calculated in real time to provide feedback support for electrotransformation parameter regulation, and the compatibility and intelligent level of the system to complex experimental conditions are enhanced, so that the efficiency of electrotransformation operation can be significantly improved.

The monitoring and control of bioprocesses is provided. The present disclosure provides the ability to generate generic calibration models, independent of cell line, using inline Raman probes to monitor changes in glucose, lactate, glutamate, ammonium, viable cell concentration (VCC), total cell concentration (TCC) and product concentration. Calibration models were developed from cell culture using two different CHOK1SV GS-KO™ cell lines producing different monoclonal antibodies (mAbs). Developed predictive models, qualified using an independent CHOK1SV GS-KO™ cell line not used in calibration, measured changes in glucose, lactate, ammonium, VCC, and TCC with minor prediction errors over the course of cell culture with minimal cell line dependence. The development of these generic models allows the application of spectroscopic PAT techniques in a clinical manufacturing environment, where processes are typically run once or twice in GMP manufacturing based on a common platform process.

A cell processing centrifugal cup, comprising a cover body (1) and a cup body (2), the cover body (1) being used for closing an upper opening of the cup body (2), and the cup body (2) comprising an upper cup body (21) and a lower cup body (22) which are integrally formed. The centrifugal cup is internally provided with a circular sleeve (7); the upper end of the circular sleeve (7) is fixedly connected to a lower portion of the cover body (1), and the lower end thereof extends into the lower cup body (22) and is suspended; and a plurality of stirring paddles (6) are mounted outside the circular sleeve (7). The upper cup body (21) is of a cylindrical structure or a circular truncated cone structure having a large top and a small bottom; the inner wall surface of the lower cup body (22) gradually inclines towards the center from top to bottom; and the inner wall surface of the lower cup body (22) is smooth or is provided with at least one protrusion (221), the protrusion (221) being circumferentially arranged along the inner wall surface of the lower cup body (22). The centrifugal cup uses the cup body structure the inner wall of which has a certain degree of inclination, which, compared with existing straight cylindrical cups, helps to gather cells, reduces mutual pressing between cells and improves activity of the cells. The inner wall surface of the lower cup body is provided with a plurality of protrusions, and the plurality of stirring paddles are mounted outside the circular sleeve, so that aggregated cells can be gently dispersed into single suspension cells within a short time period.

The observation apparatus of the present invention is an observation apparatus for observing a cell suspension containing multiple cells. The observation apparatus comprises: at least one irradiation unit disposed opposite to the cell suspension, which irradiates the cell suspension with irradiation light having at least one wavelength in the range of 650 nm to 850 nm and 1060 nm to 1090 nm; at least one light-receiving unit disposed opposite to the cell suspension, which receives emitted light emitted from the cell suspension by irradiating it with the irradiation light; and a resolution unit communicatively connected to the light-receiving unit, which determines parameters related to the cell concentration in the cell suspension based on the intensity of the emitted light.