65 results about "Cell concentration" patented technology

The cell treatment centrifugal cup comprises a cover body and a cup body, the cover body is used for sealing an upper opening of the cup body, the cup body comprises an upper cup body and a lower cup body which are integrally formed, a circular sleeve is arranged in the centrifugal cup, the upper end of the circular sleeve is fixedly connected with the lower portion of the cover body, and the lower end of the circular sleeve extends into the lower cup body and is suspended; a plurality of stirring paddles are mounted outside the circular sleeve; the upper cup body is of a cylindrical or big-end-up circular truncated cone structure, the inner wall face of the lower cup body gradually inclines towards the center from top to bottom, at least one protrusion is arranged on the inner wall face of the lower cup body, and the protrusions are arranged in the circumferential direction of the inner wall face of the lower cup body.

The present disclosure relates to methods of determining percentage viability of a cell composition comprising cell aggregates. Such methods may include providing a first aliquot of cells and a second aliquot of cells, wherein the first aliquot of cells and the second aliquot of cells have about the same concentration of cells, measuring a total number of live cells in the first aliquot of cells; measuring a total number of cells in the second aliquot of cells, and determining the percentage viability of the cell composition as a ratio between the total number of live cells in the first aliquot of cells and the total number cells in the second aliquot of cells.

Provided are methods for the creation of ultra-high density cryopreserved cell banks. In certain embodiments, these methods employ altered perfusion culture techniques that allow for production of ultra-high density cell cultures that can be cryopreserved at unexpectedly high cell densities without the need for any cell concentration steps, while retaining excellent cell viability and quality.

The utility model provides a uniform filling device for stem cell preparations, which relates to the field of stem cell preparation filling, and comprises an appliance body, a sterile bottle, a quick connection mechanism, a peristaltic pump, a filling needle, a penicillin bottle and a liquid storage bag body, and the liquid storage bag body comprises a disposable liquid storage bag; according to the liquid storage bag, the liquid storage bag body is assembled in the B + A level environment, the whole liquid storage bag is subjected to irradiation sterilization, the risk of visible foreign matter is reduced, the contamination risk is reduced to the minimum, the whole liquid storage bag is a closed pipeline, the contamination risk caused by manual operation is avoided, and the service life of the liquid storage bag is prolonged. In the filling process, the defects that each bottle of preparation finished product filled by a liquid storage bottle and a bottle shaking device is unstable in volume and large in error are overcome, after filling is finished, almost no stem cell preparation is left in the liquid storage bag, the loss of the stem cell preparation is reduced, and the filling efficiency is improved by filling through a filling machine. And the loading stability and the cell concentration uniformity of each bottle of stem cell preparation finished product are superior to those of manual loading.

This product is a work cell for cell therapy manufacturing, automating the entire manufacturing process, including cell concentration, separation, activation, gene transfer using viral vectors or electroporation, expansion culture, filling, finishing, and formulation. This product can provide a self-contained cleanroom environment with a robotic work cell, all within the size of a conference room or a small pickup truck.

The application discloses a preparation method of CCR2+ gamma delta T cells with anti-tumor activity and migration ability, and belongs to the technical field of cells; the method comprises the following steps: step (1), PBMC of peripheral blood of a healthy person is extracted, inoculated into complete culture medium, and added with zoledronic acid, IL-15 and metformin, and then is cultured in a T25 culture bottle; step (2), complete culture medium is supplemented, the cell concentration is adjusted, and then the cells are transferred into a T75 culture bottle for culture; step (3), complete culture medium is supplemented, the cell concentration is adjusted, glucocorticoid and nicotinamide are added, and then the cells are transferred into a T150 culture bottle for culture; step (4), cells are collected at D6, rinsed with normal saline, resuspended with complete culture medium, the cell concentration is adjusted, and then the cells are inoculated into a T300 culture bottle for culture; and step (5), complete culture medium is supplemented every two days from D8 to D16, the cell concentration is maintained, and then the cells are harvested at D16 to obtain CCR2+ gamma delta T cells.

A biological fluid processing device includes pump and valve systems, a controller, and an optical detection assembly. During a biological fluid processing procedure, the controller controls the operation of the pump and valve systems to separate a cell-containing fluid from the blood of a blood source and controls the optical detection assembly to emit light through the cell-containing fluid. The controller receives signals from the optical detection assembly that are indicative of the intensity of light that has passed through the cell-containing fluid. The controller receives or calculates an adjustment factor that is derived from historical data reflecting a cell collection procedure previously executed for the same blood source in which the same type of cell was collected. The controller determines an adjusted cell concentration of the cell-containing fluid based at least in part on the signals from the light detector and the adjustment factor.

A method, apparatus, and system for calculating cell concentration are disclosed. The method includes: determining an effective measurement region of a cell container, the cell container including a first encapsulation layer, a second encapsulation layer, and a solution cavity disposed between the first and second encapsulation layers; a first positioning mark is disposed on one side of the first encapsulation layer facing the solution cavity, and a second positioning mark is disposed on one side of the second encapsulation layer facing the solution cavity; dividing the effective measurement region into multiple sub-regions; focusing and acquiring an image of each sub-region; detecting the number of cells in each image and summing the number of cells in multiple images to obtain the total number of cells in the effective measurement region; focusing on the first and second positioning marks to obtain a first original cavity thickness; multiplying the first original cavity thickness by a preset adjustment coefficient to obtain an adjusted cavity thickness; calculating the volume of the effective measurement region based on the adjusted cavity thickness; and calculating the cell concentration based on the total number of cells and the volume of the effective measurement region.

The application discloses a polar microorganism separation and culture method based on a droplet microfluidic technology. The method comprises the following steps: obtaining a polar sample and performing oscillation extraction at low temperature to obtain a sample suspension; determining a plate cultivable rate R through a solid plate culture method; based on the R and a preset target number of cultivable cells N, calculating a target total number of cells T required by a droplet microfluidic experiment, and preparing a sample with a corresponding cell concentration for machine loading; generating single-cell-wrapped microdroplets through a droplet microfluidic system for the sample for machine loading, and performing long-term culture at low temperature; and screening positive droplets and purifying strains after the culture. Through the cultivable rate feedback, the sample concentration for the microfluidic loading is precisely optimized, the separation efficiency of the polar difficult-to-culture microorganism is significantly improved, the inter-species competition inhibition of the traditional plate is broken, and the diversity and novel group proportion of the obtained strains are greatly improved.

The invention provides a cell perfusion culture method and device, and the method comprises the steps: configuring a rotatable container capable of accommodating liquid, the container being provided with a first opening located in a bottom central region and a second opening located in a top central region; a cell-containing culture solution in a cell culture tank is injected into the container through the first opening for feeding; the container is controlled to rotate, and the cells are pushed to the position close to the container wall of the container through centrifugal force; liquid is continuously discharged from the second opening, so that continuous concentration of the cells is realized; the cell concentration is detected in real time, and when the cell concentration reaches a preset value, feeding, rotating and discharging operation is stopped; the conveying direction of the first opening is switched, the concentrated cells are conveyed back to the cell culture tank, perfusion culture is achieved, efficient, low-shear, low-cost and sustainable cell concentration and separation are achieved, perfusion stability and productivity are improved, and the cell concentration and separation device is suitable for large-scale cell production and industrial biopharmaceutical processes.

The invention provides novel sample chambers, units and multi-well plates, and systems and methods thereof, for built-in measurement assurance of cell counting methods and calibrated and / or quality-assured measurement and analysis of diverse types of biological cells, e.g., cell count, cell size, cell concentration, cell sub-population, cell morphology, cell viability, etc.

1. The name of the design product: fluorescent in situ imaging cell analyzer. 2. The use of the design product: the design product is used for measuring cell concentration and activity, and belongs to the field of biological automatic analysis instrument. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

This invention discloses a method for large-scale expansion of MSC cells using 3D culture, comprising the following steps: MSC seed cell resuscitation; MSC seed cell planar culture; MSC seed cell planar expansion; MSC seed cell concentration and washing; primary scale-up culture of MSC seed cells; MSC cell washing and concentration; MSC seed cell scale-up culture; microcarrier lysis and cell suspension collection; MSC cell washing and concentration; MSC cell aliquoting and cryopreservation. The advantages of this invention are: it achieves highly efficient scale-up of MSC cells from 2D planar culture to a 3D microcarrier bioreactor, enabling cell expansion to reach more than 20-fold (primary scale-up culture) to more than 200-fold (secondary scale-up culture), while maintaining cell viability >95%.

To provide an improved verification method for verifying the performance of a fluorescence-based analysis system.SOLUTION: (a) negatively staining cells in a first portion of a standard sample comprising target cells expressing a target cell marker; (b) positively staining target cells in a second portion of the standard sample; (c) passing the first and second portions of the standard sample through an instrument to obtain a fluorescence measurement indicative of target cell concentration; (d) based on the fluorescence measurement obtained in (c), each diluted sample having a nominal concentration of target cells, each nominal cell concentration being greater than the concentration of target cells indicated by the fluorescence measurement of the negatively stained first portion in (a); Preparing a dilution series comprising a plurality of diluted samples, wherein the nominal concentration in each diluted sample is different from the nominal concentration in each of the remaining diluted samples; (e) passing the dilution series of (d) through the instrument and obtaining a fluorescence measurement; and (f) comparing the nominal cell concentration of (d) and the fluorescence measurement of (e) for each diluted sample to quantify the performance of the staining method in the instrument.SELECTED DRAWING: Figure 1

The invention provides a cell concentration method and device. The device comprises a driving unit, a horizontally-arranged rotating plate, a hose penetrating through the rotating plate, a pipe body connected with the hose and a conveying assembly composed of a feeding pipe, a discharging pipe, a feeding valve and a discharging valve. And the control unit realizes continuous centrifugal concentration of liquid containing cells by coordinating the rotating speed of the driving unit and the working modes of the feeding valve and the discharging valve. The hose keeps an internal flow channel unobstructed through the anti-distortion characteristic of the hose in the rotating process, the hose body tends to be in a horizontal state along with rotation of the rotating plate to form a stable centrifugal field, and the conveying assembly achieves continuous separation and recovery of cell sediment through multi-mode flow speed regulation and control. According to the technical scheme, through the anti-twisting property of the hose and the combined structure of the horizontal pipe body, the device structure is simplified, and the manufacturing cost is reduced.

The invention relates to the technical field of waste lithium battery positive electrode material recovery, and discloses a method for recovering a ternary lithium positive electrode material through photocatalytic microbiological leaching and electrodeposition. The method specifically comprises the following steps: (1) pretreating to obtain a positive electrode material with the purity of more than or equal to 95%; (2) culturing by adopting a 9K liquid culture medium to obtain an activated bacterial solution with the thallus concentration of more than 1 * 10 < 8 > / mL (the ratio of acidithiobacillus acidophilus to thiobacillus ferrooxidans to leptospirillum is 5: 3: 2); (3) mixing a TiO2 / g-C3N4 heterojunction photocatalyst with a positive electrode material, adding an activated bacterial liquid, and carrying out synergistic leaching under the condition that the solid-to-liquid ratio is 1: 15, wherein the leaching rates of Ni, Co and Mn within 72 hours respectively reach 98.3 + / -0.5%, 96.7 + / -0.3% and 94.2 + / -0.4%; and (4) after the leachate is filtered, electro-deposition is carried out by adopting a method of adjusting electrolytic potential and pH step by step, cobalt, nickel and manganese are sequentially and selectively recovered, and lithium is recovered in the form of Li2CO3 (the purity is 99.7%). Compared with the prior art, the method has the advantages that the metal recovery rate is increased while the acid consumption and the waste water and waste gas emission are effectively reduced, and the photocatalyst and the microbial strains can be recycled.

The application discloses a method for improving the cadmium ion adsorption capacity of Escherichia coli, and belongs to the technical field of genetic engineering. E. coli BL21 to Cd 2+ , and the unit point mutation of T16C, K19C and S45C to CeMT2 can significantly improve the Cd 2+ binding capacity of CeMT2, and the cell Cd 2+ concentration of the T16C, K19C and S45C mutants is increased by 1.32 times, 1.50 times and 1.41 times, respectively. E. coli The recombinant expression of CeMT2 (K19C) and LmCadC in BL21 can obviously improve the cadmium ion adsorption capacity of Escherichia coli, and the maximum adsorption capacity reaches 8.5 mg / g.

The application discloses an impedance measurement method and device for intelligent control of an electrotransformation instrument, and relates to the technical field of impedance measurement. The type of an electrotransformation cup and the cell concentration of a sample solution are acquired to determine a switch control signal of a resistance matrix; a sine alternating current excitation of a target frequency is applied to the electrotransformation cup; the voltage on both sides of the electrotransformation cup and the voltage on both sides of the resistance matrix are detected to obtain a voltage signal; and the complex impedance of the sample solution is calculated according to the voltage signal and the resistance matrix. By acquiring the type of the electrotransformation cup and the cell concentration, the configuration of the resistance matrix is automatically controlled to realize adaptive measurement of different impedance ranges. The problems of signal saturation or weakness are effectively avoided, and the linear range and signal-to-noise ratio of measurement are improved. The complex impedance is calculated in real time to provide feedback support for electrotransformation parameter regulation, and the compatibility and intelligent level of the system to complex experimental conditions are enhanced, so that the efficiency of electrotransformation operation can be significantly improved.

A cell processing centrifugal cup, comprising a cover body (1) and a cup body (2), the cover body (1) being used for closing an upper opening of the cup body (2), and the cup body (2) comprising an upper cup body (21) and a lower cup body (22) which are integrally formed. The centrifugal cup is internally provided with a circular sleeve (7); the upper end of the circular sleeve (7) is fixedly connected to a lower portion of the cover body (1), and the lower end thereof extends into the lower cup body (22) and is suspended; and a plurality of stirring paddles (6) are mounted outside the circular sleeve (7). The upper cup body (21) is of a cylindrical structure or a circular truncated cone structure having a large top and a small bottom; the inner wall surface of the lower cup body (22) gradually inclines towards the center from top to bottom; and the inner wall surface of the lower cup body (22) is smooth or is provided with at least one protrusion (221), the protrusion (221) being circumferentially arranged along the inner wall surface of the lower cup body (22). The centrifugal cup uses the cup body structure the inner wall of which has a certain degree of inclination, which, compared with existing straight cylindrical cups, helps to gather cells, reduces mutual pressing between cells and improves activity of the cells. The inner wall surface of the lower cup body is provided with a plurality of protrusions, and the plurality of stirring paddles are mounted outside the circular sleeve, so that aggregated cells can be gently dispersed into single suspension cells within a short time period.

The observation apparatus of the present invention is an observation apparatus for observing a cell suspension containing multiple cells. The observation apparatus comprises: at least one irradiation unit disposed opposite to the cell suspension, which irradiates the cell suspension with irradiation light having at least one wavelength in the range of 650 nm to 850 nm and 1060 nm to 1090 nm; at least one light-receiving unit disposed opposite to the cell suspension, which receives emitted light emitted from the cell suspension by irradiating it with the irradiation light; and a resolution unit communicatively connected to the light-receiving unit, which determines parameters related to the cell concentration in the cell suspension based on the intensity of the emitted light.

The invention relates to the field of medical instruments, and discloses a pressure-flow rate cooperative control method and system, and the method comprises the steps: obtaining standardized operation data of a flow cytometer fluid path; constructing a liquid path sparse Bayesian causal graph structure; performing feature screening and weight addition on the sparse Bayesian causal graph structure of the liquid path to obtain a multi-level risk probability of each time window; adjusting the importance coefficient of nodes in the liquid path sparse Bayesian causality graph structure by using the multi-level risk probability; using a liquid path sparse Bayesian causal graph structure to constrain the reinforcement learning strategy space to obtain a liquid path parameter adjustment suggestion; a liquid path parameter adjustment suggestion is combined with PID control to form a pressure and flow rate dual-target adaptive control instruction set; and executing the adaptive control instruction set, and driving continuous evolution of the liquid path parameter adjustment suggestions. According to the invention, the flow velocity is stable, the repeatability of cell concentration, absolute counting and signal intensity is improved, and high-quality quantitative analysis is supported.

This disclosure relates to the adjustment of the measurement of cellular concentration in a fluid determined by a sensor. [Solution] The biofluid processing apparatus 10 includes a pump system and a valve system, a control unit, and an optical detection assembly. During the execution of the biofluid processing procedure, the control unit controls the operation of the pump system and the valve system to separate the cell-containing fluid from the blood of the blood supply source, and further controls the optical detection assembly to irradiate the cell-containing fluid with light. The control unit receives a signal from the optical detection assembly indicating the intensity of the light transmitted through the cell-containing fluid. The control unit receives or calculates an adjustment coefficient derived from historical data reflecting a cell collection procedure previously performed on the same blood supply source from which the cells were collected. The control unit determines the adjusted cell concentration of the cell-containing fluid based at least in part on the signal from the photodetector and the adjustment coefficient.

The invention relates to the technical field of microbial culture, in particular to a strain morphology screening system and method based on an image technology. The method comprises the following steps: diluting a to-be-screened strain to a predetermined cell concentration, and injecting the diluted strain into a micro-chamber array of a micro-fluidic chip; periodically collecting images of the micro-cavity array, measuring morphological parameters of cells in each micro-cavity, and recording a collection moment; calculating the variable quantity of each morphological parameter of the same micro-chamber between two adjacent acquisition moments, performing weighted summation on the variable quantity of each morphological parameter according to a preset weight, and dividing the sum by the time interval to obtain the morphological change speed of each micro-chamber; and when the morphological change speed of the micro-chamber exceeds a preset speed threshold, adding the micro-chamber to a key monitoring list. According to the invention, the micro-chamber array of the micro-fluidic chip is combined with morphological parameter extraction and machine learning classification, and dynamic monitoring and accurate identification are carried out on the strain morphological transformation process, so that efficient screening of target strains is realized.

The invention provides an automatic parallel culture method, system and equipment of cell parallel reaction equipment, and relates to the field of cell culture and biological reaction process control, and the method comprises the following steps: initializing culture parameters of a plurality of reaction tanks; in the culture process, carrying out on-line or quasi-on-line detection on the cell concentration, dissolved oxygen, pH and temperature in the reaction tank; based on a detection result, calculating a liquid change strategy parameter and / or a gas adjustment parameter of each reaction tank by the control system; according to a calculation result, independently executing liquid change operation and / or gas flow regulation operation on each reaction tank through automatic equipment; by repeating the steps, automatic, parallel and continuous culture of the multiple reaction tanks is realized. According to the invention, a special cell interception device is not needed, high-throughput, low-cost and full-automatic continuous culture can be realized under the condition of small volume, the reactor culture representativeness and high-throughput screening efficiency are both realized, and the method is suitable for application scenes such as cell amplification, differentiation culture and process optimization.

1. The name of the design product: online cell counter (CellPure). 2. The use of the design product: for real-time online detection of cell concentration, viability and size. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

The present invention relates to A semi- quantitative flow cytometry method for measuring the frequency and / or the cell concentration of one or more mononuclear cell subsets in a biological sample, wherein the subset of mononuclear cells is detected by a set of markers selected from the group of: Viability, CD45, CD66b, CD19, CD3, CD4, CD8, HLA-DR, CD279, Ki67, CD45RA and CD197, wherein said frequency and / or the cell count are determined with an inter-assay coefficient of variation (CV) of less than or equal to 20%.

The invention discloses a method for high-density amplification of feeder layer cells and application thereof. According to the method, the time for transferring the feeder layer cells to the high-density culture bottle in the feeder layer cell resuscitation and multiplication culture stage process is defined, and the cell concentration and the liquid changing and supplementing mode of feeder layer cell high-density multiplication culture are controlled. According to the method for high-density amplification of the feeder layer cells, on the premise that the quality of the feeder layer cells is guaranteed, the consumption of a culture medium used for producing the same number of feeder layer cells is reduced by 50%, the operation steps are remarkably simplified, and the inter-batch stability is higher.

Provided herein are methods for using particle counting to determine live cell concentration, total cell concentration, cell viability and biomass of cell aggregate compositions, such as for accurate cell dosing for clinical applications.

A method of determining a cell concentration of a subject fluid includes first determining an unadjusted cell concentration of each of a plurality of fluids based on an intensity of light emitted through the fluid and a correlation curve derived using a first cell counter. A measured cell concentration obtained for each fluid from a second cell counter is then plotted against the unadjusted cell concentration to create a curve represented by an equation that is selected to be used as an adjustment equation. Light is then emitted through the subject fluid, with at least a portion of the light exiting the subject fluid being received. An unadjusted cell concentration of the subject fluid is determined based on the correlation curve and the intensity of the received light. The adjustment equation is then applied to the unadjusted concentration to determine an adjusted cell concentration of the subject fluid.