32 results about "Cell concentration" patented technology

Provided are methods for the creation of ultra-high density cryopreserved cell banks. In certain embodiments, these methods employ altered perfusion culture techniques that allow for production of ultra-high density cell cultures that can be cryopreserved at unexpectedly high cell densities without the need for any cell concentration steps, while retaining excellent cell viability and quality.

This product is a work cell for cell therapy manufacturing, automating the entire manufacturing process, including cell concentration, separation, activation, gene transfer using viral vectors or electroporation, expansion culture, filling, finishing, and formulation. This product can provide a self-contained cleanroom environment with a robotic work cell, all within the size of a conference room or a small pickup truck.

The application discloses a preparation method of CCR2+ gamma delta T cells with anti-tumor activity and migration ability, and belongs to the technical field of cells; the method comprises the following steps: step (1), PBMC of peripheral blood of a healthy person is extracted, inoculated into complete culture medium, and added with zoledronic acid, IL-15 and metformin, and then is cultured in a T25 culture bottle; step (2), complete culture medium is supplemented, the cell concentration is adjusted, and then the cells are transferred into a T75 culture bottle for culture; step (3), complete culture medium is supplemented, the cell concentration is adjusted, glucocorticoid and nicotinamide are added, and then the cells are transferred into a T150 culture bottle for culture; step (4), cells are collected at D6, rinsed with normal saline, resuspended with complete culture medium, the cell concentration is adjusted, and then the cells are inoculated into a T300 culture bottle for culture; and step (5), complete culture medium is supplemented every two days from D8 to D16, the cell concentration is maintained, and then the cells are harvested at D16 to obtain CCR2+ gamma delta T cells.

A biological fluid processing device includes pump and valve systems, a controller, and an optical detection assembly. During a biological fluid processing procedure, the controller controls the operation of the pump and valve systems to separate a cell-containing fluid from the blood of a blood source and controls the optical detection assembly to emit light through the cell-containing fluid. The controller receives signals from the optical detection assembly that are indicative of the intensity of light that has passed through the cell-containing fluid. The controller receives or calculates an adjustment factor that is derived from historical data reflecting a cell collection procedure previously executed for the same blood source in which the same type of cell was collected. The controller determines an adjusted cell concentration of the cell-containing fluid based at least in part on the signals from the light detector and the adjustment factor.

A method, apparatus, and system for calculating cell concentration are disclosed. The method includes: determining an effective measurement region of a cell container, the cell container including a first encapsulation layer, a second encapsulation layer, and a solution cavity disposed between the first and second encapsulation layers; a first positioning mark is disposed on one side of the first encapsulation layer facing the solution cavity, and a second positioning mark is disposed on one side of the second encapsulation layer facing the solution cavity; dividing the effective measurement region into multiple sub-regions; focusing and acquiring an image of each sub-region; detecting the number of cells in each image and summing the number of cells in multiple images to obtain the total number of cells in the effective measurement region; focusing on the first and second positioning marks to obtain a first original cavity thickness; multiplying the first original cavity thickness by a preset adjustment coefficient to obtain an adjusted cavity thickness; calculating the volume of the effective measurement region based on the adjusted cavity thickness; and calculating the cell concentration based on the total number of cells and the volume of the effective measurement region.

1. The name of the design product: fluorescent in situ imaging cell analyzer. 2. The use of the design product: the design product is used for measuring cell concentration and activity, and belongs to the field of biological automatic analysis instrument. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

This invention discloses a method for large-scale expansion of MSC cells using 3D culture, comprising the following steps: MSC seed cell resuscitation; MSC seed cell planar culture; MSC seed cell planar expansion; MSC seed cell concentration and washing; primary scale-up culture of MSC seed cells; MSC cell washing and concentration; MSC seed cell scale-up culture; microcarrier lysis and cell suspension collection; MSC cell washing and concentration; MSC cell aliquoting and cryopreservation. The advantages of this invention are: it achieves highly efficient scale-up of MSC cells from 2D planar culture to a 3D microcarrier bioreactor, enabling cell expansion to reach more than 20-fold (primary scale-up culture) to more than 200-fold (secondary scale-up culture), while maintaining cell viability >95%.

The application discloses a method for improving the cadmium ion adsorption capacity of Escherichia coli, and belongs to the technical field of genetic engineering. E. coli BL21 to Cd 2+ , and the unit point mutation of T16C, K19C and S45C to CeMT2 can significantly improve the Cd 2+ binding capacity of CeMT2, and the cell Cd 2+ concentration of the T16C, K19C and S45C mutants is increased by 1.32 times, 1.50 times and 1.41 times, respectively. E. coli The recombinant expression of CeMT2 (K19C) and LmCadC in BL21 can obviously improve the cadmium ion adsorption capacity of Escherichia coli, and the maximum adsorption capacity reaches 8.5 mg / g.

A cell processing centrifugal cup, comprising a cover body (1) and a cup body (2), the cover body (1) being used for closing an upper opening of the cup body (2), and the cup body (2) comprising an upper cup body (21) and a lower cup body (22) which are integrally formed. The centrifugal cup is internally provided with a circular sleeve (7); the upper end of the circular sleeve (7) is fixedly connected to a lower portion of the cover body (1), and the lower end thereof extends into the lower cup body (22) and is suspended; and a plurality of stirring paddles (6) are mounted outside the circular sleeve (7). The upper cup body (21) is of a cylindrical structure or a circular truncated cone structure having a large top and a small bottom; the inner wall surface of the lower cup body (22) gradually inclines towards the center from top to bottom; and the inner wall surface of the lower cup body (22) is smooth or is provided with at least one protrusion (221), the protrusion (221) being circumferentially arranged along the inner wall surface of the lower cup body (22). The centrifugal cup uses the cup body structure the inner wall of which has a certain degree of inclination, which, compared with existing straight cylindrical cups, helps to gather cells, reduces mutual pressing between cells and improves activity of the cells. The inner wall surface of the lower cup body is provided with a plurality of protrusions, and the plurality of stirring paddles are mounted outside the circular sleeve, so that aggregated cells can be gently dispersed into single suspension cells within a short time period.

The observation apparatus of the present invention is an observation apparatus for observing a cell suspension containing multiple cells. The observation apparatus comprises: at least one irradiation unit disposed opposite to the cell suspension, which irradiates the cell suspension with irradiation light having at least one wavelength in the range of 650 nm to 850 nm and 1060 nm to 1090 nm; at least one light-receiving unit disposed opposite to the cell suspension, which receives emitted light emitted from the cell suspension by irradiating it with the irradiation light; and a resolution unit communicatively connected to the light-receiving unit, which determines parameters related to the cell concentration in the cell suspension based on the intensity of the emitted light.

The invention relates to the field of medical instruments, and discloses a pressure-flow rate cooperative control method and system, and the method comprises the steps: obtaining standardized operation data of a flow cytometer fluid path; constructing a liquid path sparse Bayesian causal graph structure; performing feature screening and weight addition on the sparse Bayesian causal graph structure of the liquid path to obtain a multi-level risk probability of each time window; adjusting the importance coefficient of nodes in the liquid path sparse Bayesian causality graph structure by using the multi-level risk probability; using a liquid path sparse Bayesian causal graph structure to constrain the reinforcement learning strategy space to obtain a liquid path parameter adjustment suggestion; a liquid path parameter adjustment suggestion is combined with PID control to form a pressure and flow rate dual-target adaptive control instruction set; and executing the adaptive control instruction set, and driving continuous evolution of the liquid path parameter adjustment suggestions. According to the invention, the flow velocity is stable, the repeatability of cell concentration, absolute counting and signal intensity is improved, and high-quality quantitative analysis is supported.

This disclosure relates to the adjustment of the measurement of cellular concentration in a fluid determined by a sensor. [Solution] The biofluid processing apparatus 10 includes a pump system and a valve system, a control unit, and an optical detection assembly. During the execution of the biofluid processing procedure, the control unit controls the operation of the pump system and the valve system to separate the cell-containing fluid from the blood of the blood supply source, and further controls the optical detection assembly to irradiate the cell-containing fluid with light. The control unit receives a signal from the optical detection assembly indicating the intensity of the light transmitted through the cell-containing fluid. The control unit receives or calculates an adjustment coefficient derived from historical data reflecting a cell collection procedure previously performed on the same blood supply source from which the cells were collected. The control unit determines the adjusted cell concentration of the cell-containing fluid based at least in part on the signal from the photodetector and the adjustment coefficient.

The invention relates to the technical field of microbial culture, in particular to a strain morphology screening system and method based on an image technology. The method comprises the following steps: diluting a to-be-screened strain to a predetermined cell concentration, and injecting the diluted strain into a micro-chamber array of a micro-fluidic chip; periodically collecting images of the micro-cavity array, measuring morphological parameters of cells in each micro-cavity, and recording a collection moment; calculating the variable quantity of each morphological parameter of the same micro-chamber between two adjacent acquisition moments, performing weighted summation on the variable quantity of each morphological parameter according to a preset weight, and dividing the sum by the time interval to obtain the morphological change speed of each micro-chamber; and when the morphological change speed of the micro-chamber exceeds a preset speed threshold, adding the micro-chamber to a key monitoring list. According to the invention, the micro-chamber array of the micro-fluidic chip is combined with morphological parameter extraction and machine learning classification, and dynamic monitoring and accurate identification are carried out on the strain morphological transformation process, so that efficient screening of target strains is realized.

The invention provides an automatic parallel culture method, system and equipment of cell parallel reaction equipment, and relates to the field of cell culture and biological reaction process control, and the method comprises the following steps: initializing culture parameters of a plurality of reaction tanks; in the culture process, carrying out on-line or quasi-on-line detection on the cell concentration, dissolved oxygen, pH and temperature in the reaction tank; based on a detection result, calculating a liquid change strategy parameter and / or a gas adjustment parameter of each reaction tank by the control system; according to a calculation result, independently executing liquid change operation and / or gas flow regulation operation on each reaction tank through automatic equipment; by repeating the steps, automatic, parallel and continuous culture of the multiple reaction tanks is realized. According to the invention, a special cell interception device is not needed, high-throughput, low-cost and full-automatic continuous culture can be realized under the condition of small volume, the reactor culture representativeness and high-throughput screening efficiency are both realized, and the method is suitable for application scenes such as cell amplification, differentiation culture and process optimization.

The invention discloses a method for high-density amplification of feeder layer cells and application thereof. According to the method, the time for transferring the feeder layer cells to the high-density culture bottle in the feeder layer cell resuscitation and multiplication culture stage process is defined, and the cell concentration and the liquid changing and supplementing mode of feeder layer cell high-density multiplication culture are controlled. According to the method for high-density amplification of the feeder layer cells, on the premise that the quality of the feeder layer cells is guaranteed, the consumption of a culture medium used for producing the same number of feeder layer cells is reduced by 50%, the operation steps are remarkably simplified, and the inter-batch stability is higher.

The invention provides a cascade viscoelastic micro-fluidic chip and a method for preparing, sorting and enriching circulating tumor cells. The cascade viscoelastic micro-fluidic chip comprises a cell sorting module, a transition section and a cell concentration module. The cell sorting module is composed of an inlet I, an inlet II, an annular bifurcated branch, a straight channel section I, an amplification section, a serpentine channel I and an outlet I; the cell concentration module is composed of a second straight channel section, a first bifurcation structure, a second serpentine channel, a second outlet, a third straight channel section, a second bifurcation structure, a third serpentine channel, a third outlet, a fourth straight channel section, a third bifurcation structure, a fourth outlet and a fifth outlet. The cell sorting module and the cell concentration module are cascaded through a transition section. The invention breaks through the bottleneck that the traditional sorting technology can be compatible with downstream biological analysis only by intervening manual centrifugation and other operations at the rear end, and can provide powerful technical support for developing a tumor liquid biopsy integrated system.

A biofluidic processing apparatus, a biofluidic processing system, and a method for implementing a controller to perform a biofluidic processing procedure for a blood source are disclosed. The biofluidic processing apparatus includes a pump system and a valve system, a controller, and an optical detection assembly. During the biofluidic processing procedure, the controller controls the operation of the pump system and the valve system to separate the cell-containing fluid from the blood of the blood source, and controls the optical detection assembly to emit light through the cell-containing fluid. The controller receives a signal from the optical detection assembly indicating the intensity of light passing through the cell-containing fluid. The controller receives or calculates a modulation factor derived from historical data reflecting previous cell collection procedures performed on the same blood source with the same type of cells collected. The controller determines the regulated cell concentration of the cell-containing fluid based at least in part on the signal from the photodetector and the modulation factor.

The utility model belongs to the technical field of single-cell sterile printing separation, and particularly relates to a single-cell sterile printing separation device which comprises a separation cup, one side of the separation cup is fixedly connected with a transparent plate, the top end of the separation cup is movably connected with an upper cover, a supporting groove is formed in the upper cover, and the transparent plate is arranged in the supporting groove. A limiting groove is formed in the top of the upper cover; the rotating rod is rotated to drive the fan-shaped end of the driving plate to slide from the inner side of the arc-shaped plate, and the other end of the driving plate drives the driving rod to enter the anti-skid pad in the sliding groove, so that thrust is applied to the arc-shaped plate and the sliding groove respectively, the driving plate, the arc-shaped plate and the driving rod are matched to drive the bottom plate to rotate, and the rotating rod rotates by one circle to drive the bottom plate and the stirring rod to rotate by one circle; the stirring device is used for stirring the cell suspension so as to ensure uniform distribution of cells and prevent the cells from gathering or settling in the dilution process, so that the concentration uniformity of the diluted cells is ensured, and the influence on the precision and efficiency of cell separation is avoided.

This invention belongs to the field of biotechnology. Specifically, this invention discloses neural stem cells, their preparation methods and applications, including the following steps: (1) washing hippocampal tissue with PBS buffer, placing the washed hippocampal tissue in a digestive solution for digestion, adding a digestion termination solution to terminate digestion, centrifuging to obtain cell pellet, washing the cell pellet with PBS buffer, centrifuging, resuspending the cells in DMEM / F12 medium, and adjusting the cell concentration to 1×10⁻⁶. 4 (2) Primary culture; (3) Amplification culture. The amplification culture medium described in this invention can provide a carrier with viscosity during the culture of neural stem cells, promote the division and proliferation of neural stem cells, improve the activity and stability of neural stem cells, and increase the survival rate and cell number, and has broad application prospects.

A cell counter and diagnostic device, disclosed is a device for determining the concentration of cells of a given size in a 5 fluid sample, the sample comprising a plurality of cells, the device comprising: a light source arranged to emit light along a central axis to illuminate the plurality of cells in the fluid sample; the container is used for containing a sample; a detector arranged for receiving light at a plurality of displacements relative to the central axis, the light having passed through a plurality of cells in the fluid sample, and generating a plurality of signals, each signal being associated with and indicative of an intensity of the light received at 10 respective displacements of the plurality of displacements; and a processor arranged to: acquire a first light intensity reading at a first displacement relative to the central axis; acquiring a second light intensity reading at a second displacement relative to the central axis; determining a difference between the first light intensity reading and the second light intensity reading; determining a cell concentration based on the difference; wherein the container is one of: an effluent tube arranged to deliver 15 effluent from the patient; an effluent bag arranged for collecting effluent from the patient; and a cartridge constituting part of the automated peritoneal dialysis system. Figure 9

The application discloses a kind of intelligent mobile robot path planning method, simulates CAR-T targeted therapy, comprising the following steps: grid map, input robot starting point and end point position;Suppose that intelligent group for path planning is T cell, T cell uses octal neighborhood search at starting point, set the maximum iteration number of algorithm, T cell selects starting point and expands search eight sub-neighborhood, T cell in sub-neighborhood is elite to CAR-T cell, and the elite rate is directly proportional to the distance to end point;CAR-T cell is transferred to other sub-neighborhood with certain probability;Judge whether end point is found;If find, then current iteration ends, otherwise, the sub-neighborhood of selected cell concentration highest, continue to repeat the above octal neighborhood search process until end point is found.The initial T cell concentration of historical optimal path is enhanced in the next iteration.The application has the advantages that the search efficiency and accuracy of the algorithm are improved, a globally optimal task path can be planned for the robot in a short time, and the work efficiency is effectively improved.

This invention discloses an algorithm-assisted microfluidic-microwave integrated biosensor and its high-precision detection method, relating to the fields of biosensors and medical detection technology. The biosensor includes a microfluidic sorting module, a dual-frequency microwave sensing module, and an intelligent algorithm unit. The microfluidic sorting module achieves label-free cell sorting and enrichment through a helical microchannel. The dual-frequency microwave sensing module acquires the dual-band dielectric response of cells through two independent resonant units. The intelligent algorithm unit incorporates a dual-mode feature fusion model based on a multi-head cross-attention mechanism, which can dynamically fuse multi-dimensional sensing features. This invention solves the problems of limited sensitivity and large nonlinear concentration prediction errors in traditional single-frequency microwave sensors, achieving label-free quantitative detection of cell concentration with low error, high linearity, and strong anti-interference capabilities.

A milk analysis system (100) for counting individual somatic cells (410a, 410b) in a milk sample. The system comprises a measuring chamber (220) with an inlet (231), outlet (241), first plate (281), second plate (282), and wall element (283) forming an enclosed volume. A sensor (160) captures an image (270) through a transparent portion of the first plate. A controller (200) processes the image to determine a region of interest (275), counts individual somatic cells, applies a correction factor, calculates sample volume, and determines cell concentration. The controller controls the sensor, processes the image, counts cells, applies the correction factor, calculates volume, and determines cell concentration per volume based 10 on the corrected count and calculated volume.

The application provides a numerical simulation method and system for separating cells by using a hydrocyclone, and comprises the following steps: obtaining the concentration and surface area of cells to be separated, the number of fluid phases, and the shear stress of fluid under different shear rates; calculating the sphericity of the cells based on the surface area of the cells to be separated; obtaining the non-Newtonian fluid flow index of the fluid based on the shear stress of the fluid under different shear rates; calculating the drag force on the cells based on the sphericity of the cells and the non-Newtonian fluid flow index of the fluid; selecting different methods according to the concentration of the cells and the number of fluid phases, simulating the process of separating the cells by using a micro hydrocyclone based on the drag force, and obtaining characteristic parameters. The numerical simulation calculation is expanded from a single model to a plurality of models, which provides a guarantee for more comprehensive research and application of the micro hydrocyclone for separating cells.

Described herein are apparatuses and methods for the processing and / or measurements of chemical or biochemical samples on a digital microfluidic device. Also described are methods to configure and operate the modules for efficient processing and measurements of the samples on the device. The apparatus can be used in applications such as DNA / RNA / protein / cell concentration / purification, real-time PCR, isothermal amplification, immunoassay, cell-based assay, library preparation for NGS sequencing, etc.

The application discloses a feedback feeding control method based on multi-component concentration online detection, which is used for producing ethanol by using Saccharomyces cerevisiae and comprises the following steps: S1, in-situ online sampling is performed, and online monitoring instruments are used to detect the substrate and product concentrations in a fermentation tank in real time; S2, a kinetic model based on substrate consumption is established according to the detected substrate, product and predicted cell concentration of a soft measurement model; S3, a particle swarm algorithm is combined to optimize the parameters of the substrate consumption kinetics, and the optimized model is used as a reference track; S4, the output of a rolling optimization concentration feedback controller is optimized; and S5, the output of the controller is calculated according to a single neuron adaptive PID feedback controller based on a quadratic performance index, so that the optimal set track is quickly tracked, and the substrate concentration in the fermentation tank is maintained at a required concentration. Experimental results prove that the feedback feeding control based on the online detection of the component concentration is beneficial to the utilization rate of the substrate and the generation of the product, and meanwhile, the method can also be applied to other actual fermentations.

The utility model relates to biological experiment equipment technical field, concretely relates to a cell culture plate, the cell culture plate includes: culture plate main part, culture hole, set up in the upper surface of culture plate main part, is enclosed by bottom wall and side wall, side wall sets up in the side of culture hole, and is connected with culture plate main part, bottom wall is connected in the below of side wall, and bottom wall is the taper type of wide on the top narrow, mounting groove sets up in the inside of side wall, limit along, through mounting groove, makes detachable connection between limit along with side wall. The utility model discloses through setting limit along, increase the limiting resistance of cell liquid in culture hole in the process of oscillation or shaking, limit the fluctuation amplitude of cell liquid in culture hole in the process of oscillation or shaking, under the condition of guaranteeing the cell liquid cell concentration uniformity, avoid cell liquid to splash out culture hole, effectively guarantee the accuracy of tumor cell proliferation inhibition experiment.

The invention discloses an electrochemical cell sensor for enhancing electron transfer based on cell spreading, preparation and application. The electrochemical cell sensor comprises a first antibody / RGO (at) Ti3C2Tx cell spreading promoting unit and a FeCN (at) second antibody signal output unit, a second antibody in the FeCN and second antibody signal output unit is covalently connected with FeCN, the FeCN targets the surface of a cell membrane through the second antibody by taking monatomic Fe as a catalytic activity center, and a precipitation type TMB reaction solution is catalyzed by simulating peroxidase activity to generate TMB < + > precipitates around cells; the cell concentration is quantified by quantifying the current generated by the electroactive TMB +. The electrochemical cell sensor can enhance electric signal response by promoting cell height spreading, can be used for detecting cell concentration to realize electric signal output of single cells, and can also be used for detecting cell membrane protein.

The present disclosure provides cassettes for use in automated cell engineering systems that include cell concentration filters for reducing fluid volume of a cell sample during or following automated processing. The disclosure also provides methods of concentrating a cell population, as well as automated cell engineering systems that can utilize the cassettes and carry out the methods.

This invention discloses a method for gene transfection of fibroblasts. The method includes: obtaining a fibroblast suspension with a cell concentration of 1-2 cells / µL; preparing cell suspension droplets in a cell culture dish, each droplet consisting of 10±0.5µL of the fibroblast suspension; incubating the cell culture dish containing the cell suspension droplets in a microenvironment, and adding cell culture medium after cell adhesion; obtaining a plasmid solution containing a linearized target gene with a concentration of 150-200 ng / µL; aspirating the plasmid solution into an injection needle, adjusting the angle between the injection needle and the horizontal plane to 30-60°, and injecting it sequentially into each cell; performing single-cell culture after injection to obtain stably transfected transgenic cell clones. This method aims to obtain a cell line without selection markers while achieving high-efficiency and stable transfection.