14 results about "Cell stimulation" patented technology

The invention relates to a transcranial cell stimulation experiment model, which is characterized in that a support structure is used for supporting a skull model, the upper end of the support structure enters the skull model, a placement platform fixed on the support structure is arranged in the skull model, a cell culture dish is placed on the placement platform, two sides of the skull model are respectively provided with a stimulation coil which is obliquely distributed, and the stimulation coils are arranged on the skull model. The power supply unit is used for rapidly discharging to the two stimulation coils so as to induce generation of transient strong pulse current in the stimulation coils, and a 1T-4T strong magnetic field is formed around the stimulation coils. A transcranial cell stimulation experiment method comprises the following steps: firstly, pretreating SH-SY5Y cells, and then putting the pretreated SH-SY5Y cells into a cell culture dish; specific current waveforms are transmitted to the two stimulation coils, and the SH-SY5Y cells are stimulated through a generated electromagnetic field. The method has the beneficial effects that experimental simulation and experimental verification are realized, the understanding of a non-invasive brain stimulation technology is enriched, and a new scientific basis is provided for the treatment of related neurological and mental diseases.

The objective is to provide a novel method for improving the efficiency of osteogenic differentiation induction based on immobilized BMP-2. [Solution] A method for stimulating cells with ligand molecules, comprising a stimulation step of applying physical stimulation to the cells and / or ligand molecules, wherein the ligand molecule is bound to or interacts with a receptor protein expressed on the cells and is immobilized on a culture substrate and / or matrix molecule used for culturing the cells, the receptor protein mediates the transmission of extracellular signals to the cells based on its binding to the ligand molecule, and the extracellular signals are enhanced by the physical stimulation.

A therapeutic device for cell stimulation or cell therapy, comprising a housing (12) containing an electrode (1), a generator (3) for generating high-frequency voltage pulses, a processor unit (6) comprising a control, regulation, and calculation module, a storage unit (9), at least one operating element (5, 15), and a controllable modulator (4) by means of which the generator (3) can be controlled, wherein a voltage pulse sequence comprising a plurality of voltage pulses can be generated by means of the modulator (4), wherein the frequency and duration of the voltage pulses can be arbitrarily adjusted by means of the modulator (4), wherein the electrode (1), the generator (3), the processor unit (6), the storage unit (9), the operating element (5), and the modulator (4) are arranged in the housing (12), wherein the electrode (1) contains a glass body (27) which includes a cavity containing a gas, and wherein the electrode (1) has a first end (21).which can be coupled to the modulator (4), wherein the electrode comprises a second dome-shaped end (22), wherein the gas can be brought into the state of a non-thermal primary plasma by the voltage pulses transmitted to the electrode (1), wherein a secondary plasma can be generated by ionizing the air located in the vicinity of the second end (22) of the electrode (1).

A therapeutic device for cell stimulation or cell therapy comprises a housing which contains an electrode, a generator for generating high-frequency pulses, a processor unit comprising a control, regulation and calculation module, a memory unit, a control element, a controllable modulator, by means of which the generator can be controlled. A voltage pulse sequence comprising a plurality of voltage pulses can be generated by means of the modulator, wherein the frequency of the voltage pulse sequence can be at least partially not constant.

The present invention addresses the problem of providing a novel method for increasing bone differentiation induction efficiency based on immobilized BMP-2.　Provided is a method for stimulating a cell with a ligand molecule, the method including a stimulation step for applying a physical stimulus to the cell and / or the ligand molecule. The ligand molecule binds to or interacts with a receptor protein expressed on the cell, and is immobilized on a culture substrate and / or a matrix molecule used for culturing the cell. The receptor protein mediates the transmission of an extracellular signal to the cell on the basis of binding to the ligand molecule, and the extracellular signal is enhanced by the physical stimulus.

Some embodiments of the methods and compositions provided herein relate to the use of hapten labeled cells to stimulate chimeric antigen receptor (CAR) T cells. In some embodiments, CAR T cells can include a CAR that specifically binds to a hapten. Some embodiments relate to the in vivo or in vitro stimulation CAR T cells by hapten labeled cells.

A therapeutic device for cell stimulation or cell therapy comprises a housing accommodating electrodes, a generator for generating high-frequency pulses, a processor unit comprising a control, regulation and calculation module, a memory unit, a control element, a controllable modulator by means of which the generator can be controlled. A voltage pulse sequence comprising a plurality of voltage pulses can be generated by means of the modulator, wherein the frequency of the voltage pulse sequence can be at least partially non-constant.

The present disclosure provides cell stimulating particles and methods of using the same. Also provided are populations of cells produced using the cell-stimulating particles.

The utility model relates to the technical field of culture tubes, and discloses a quickly detachable modular triple cell stimulation culture tube which comprises a culture tube, the top of the culture tube is open, and a limiting groove is formed in one side of the top of the culture tube. According to the technical scheme, the inserting block in the pipe cap is inserted into the culture pipe, meanwhile, the inclined opening in the bottom of the inserting block continuously extrudes the wedge-shaped block in the positioning mechanism, and the wedge-shaped block is supported by the spring on the piston rod and continuously extrudes the wedge-shaped block along with the bottom of the inserting block; the wedge-shaped block can be stored in the limiting groove in the direction of the limiting block sliding block, when the wedge-shaped block corresponds to the clamping groove in the middle of the inserting block, the wedge-shaped block can be extruded out by the supporting force of the spring, the wedge-shaped block is limited in the clamping groove, the pipe cap is positioned, it is guaranteed that after an operator inserts the pipe cap to the top of the culture pipe, the culture pipe can be rapidly sealed, and the working efficiency is improved. And the pipe cap is locked through the positioning mechanism, so that the pipe cap is effectively prevented from being opened under the condition of external force.

The application discloses a cell treatment method based on sinusoidal alternating current stimulation, and the method comprises the following steps: applying sinusoidal alternating current stimulation to target stem cells, and the mitochondrial membrane potential, ATP synthesis and aerobic respiration level of the stem cells treated by the electric stimulation are improved. The application further discloses a cell stimulation device based on sinusoidal alternating current stimulation, and the device comprises a cell culture container and a planar interdigital electrode substrate placed in the cell culture container, wherein the planar interdigital electrode substrate comprises an optically transparent substrate and metal planar interdigital electrodes on the substrate. The application discloses the application of the stem cells treated by the above method or the stem cells treated by the device in the preparation of a medicine for mitochondrial transplantation treatment. The application improves the mitochondrial membrane potential, ATP synthesis capacity and aerobic respiration level of the cells by applying sinusoidal alternating current stimulation to the target stem cells.

An exemplary pulsed electric field system and method that can uniformly emit electrical pulses via use of a conductivity-controlled liquid medium to penetrate a pre-defined depth of a target tissue for a whole or region of a hollow or tubular organ to induce a tissue response. The conductivity-controlled liquid medium allows more uniform electrical pulses to be generated with less localized or focal bursts of pulses, which can reduce the required power rating of the electric generator for the pulses. The pulsed electric field system and method can be used for irreversible electroporation and subsequent exfoliation of the surface tissue, electroporation, pulsed field ablation, or cell stimulation.

A fusion protein of IL-21 cytokine and IL-21 receptor is disclosed. Also disclosed are cells comprising the cytokine-receptor fusion, and methods and compositions for the treatment of tumors utilizing the cytokine-receptor fusion and utilizing the cells expressing the cytokine-receptor fusion.

This invention relates to the use of a combination of peripheral blood concentrated cells and concentrated supernatant for the treatment of premature ovarian failure. In one aspect, the combination comprises peripheral blood concentrated cells, granulocyte-macrophage stimulating factors, a supernatant concentrate, and optional excipients; the peripheral blood concentrated cells and supernatant concentrate are prepared using an automated cell separation system and a tangential flow ultrafiltration system. The proportion of concentrated cells is such that the concentrated cells are CD45... + Cell count 4 x 10^6 cells: 10–15 ng granulocyte-macrophage stimulating factor: 75–125 μL supernatant concentrate. The invention also relates to a method for preparing the combination of the claims, comprising the step of mixing a specified amount of concentrated cells, granulocyte-macrophage stimulating factor, supernatant concentrate, and optional excipients to prepare a sterile formulation. The cell combination of the present invention exhibits excellent effects.

Provided herein are synthetic promoters, including synthetic transcriptional regulatory sequences and variant MND promoters, that can be used for controlling expression of transcribable sequences, such as chimeric antigen receptors (CARs) and T cell receptors (TCRs), such as by allowing for induction, repression, or constitutive activation of transcription of the transcribable sequence by T cell stimulation. Also provided herein are polynucleotides and vectors containing the synthetic transcriptional regulatory sequences and variant MND promoters and transcribable sequences described herein. Also provided herein are engineered T cells containing the synthetic transcriptional regulatory sequences, variant MND promoters, polynucleotides, and vectors described herein, as well as methods of making and using same.