26 results about "Cell stimulation" patented technology

The invention relates to a transcranial cell stimulation experiment model, which is characterized in that a support structure is used for supporting a skull model, the upper end of the support structure enters the skull model, a placement platform fixed on the support structure is arranged in the skull model, a cell culture dish is placed on the placement platform, two sides of the skull model are respectively provided with a stimulation coil which is obliquely distributed, and the stimulation coils are arranged on the skull model. The power supply unit is used for rapidly discharging to the two stimulation coils so as to induce generation of transient strong pulse current in the stimulation coils, and a 1T-4T strong magnetic field is formed around the stimulation coils. A transcranial cell stimulation experiment method comprises the following steps: firstly, pretreating SH-SY5Y cells, and then putting the pretreated SH-SY5Y cells into a cell culture dish; specific current waveforms are transmitted to the two stimulation coils, and the SH-SY5Y cells are stimulated through a generated electromagnetic field. The method has the beneficial effects that experimental simulation and experimental verification are realized, the understanding of a non-invasive brain stimulation technology is enriched, and a new scientific basis is provided for the treatment of related neurological and mental diseases.

The objective is to provide a novel method for improving the efficiency of osteogenic differentiation induction based on immobilized BMP-2. [Solution] A method for stimulating cells with ligand molecules, comprising a stimulation step of applying physical stimulation to the cells and / or ligand molecules, wherein the ligand molecule is bound to or interacts with a receptor protein expressed on the cells and is immobilized on a culture substrate and / or matrix molecule used for culturing the cells, the receptor protein mediates the transmission of extracellular signals to the cells based on its binding to the ligand molecule, and the extracellular signals are enhanced by the physical stimulation.

The invention discloses a novel immune enhancement system, which comprises a specific TLR4 agonistic polypeptide and a CD40 single-domain antibody. According to the polypeptide, a KLAKLAK core module is adopted, penetrating peptide and hydrophobic groups are modified, and an NF-kappa B / MAPK pathway can be effectively activated; the antibody is obtained through shark immune screening and has high affinity to CD40. When in combined application, the GM-CSF / IL-4-containing medium synergistically promotes the maturation of dendritic cells, so that the expression of CD80 / CD86 / MHC-II is improved by more than 2 times, the secretion amount of IL-12 reaches 165 pg / mL, and the proliferation rate of T cells is improved to 48.6%. Animal experiments show that the T cell stimulation index of a combined group reaches 6.2, and the serum IFN-gamma level is improved by 9 times. The composition provided by the invention can significantly enhance the DC vaccine efficacy, and has important application value in the field of tumor immunotherapy.

A therapeutic device for cell stimulation or cell therapy, comprising a housing (12) containing an electrode (1), a generator (3) for generating high-frequency voltage pulses, a processor unit (6) comprising a control, regulation, and calculation module, a storage unit (9), at least one operating element (5, 15), and a controllable modulator (4) by means of which the generator (3) can be controlled, wherein a voltage pulse sequence comprising a plurality of voltage pulses can be generated by means of the modulator (4), wherein the frequency and duration of the voltage pulses can be arbitrarily adjusted by means of the modulator (4), wherein the electrode (1), the generator (3), the processor unit (6), the storage unit (9), the operating element (5), and the modulator (4) are arranged in the housing (12), wherein the electrode (1) contains a glass body (27) which includes a cavity containing a gas, and wherein the electrode (1) has a first end (21).which can be coupled to the modulator (4), wherein the electrode comprises a second dome-shaped end (22), wherein the gas can be brought into the state of a non-thermal primary plasma by the voltage pulses transmitted to the electrode (1), wherein a secondary plasma can be generated by ionizing the air located in the vicinity of the second end (22) of the electrode (1).

A therapeutic device for cell stimulation or cell therapy comprises a housing which contains an electrode, a generator for generating high-frequency pulses, a processor unit comprising a control, regulation and calculation module, a memory unit, a control element, a controllable modulator, by means of which the generator can be controlled. A voltage pulse sequence comprising a plurality of voltage pulses can be generated by means of the modulator, wherein the frequency of the voltage pulse sequence can be at least partially not constant.

The present invention addresses the problem of providing a novel method for increasing bone differentiation induction efficiency based on immobilized BMP-2.　Provided is a method for stimulating a cell with a ligand molecule, the method including a stimulation step for applying a physical stimulus to the cell and / or the ligand molecule. The ligand molecule binds to or interacts with a receptor protein expressed on the cell, and is immobilized on a culture substrate and / or a matrix molecule used for culturing the cell. The receptor protein mediates the transmission of an extracellular signal to the cell on the basis of binding to the ligand molecule, and the extracellular signal is enhanced by the physical stimulus.

The present invention provides a composition of mesenchymal stem cells derived from autologous fat, a preparation method thereof, and an application thereof, belonging to the technical field of medical adjuvants. Mesenchymal stem cells are isolated from autologous fat particles and cultured in a stem cell culture medium supplemented with cell stimulating particles and fetal bovine serum. Stem cells and extracellular matrix are obtained by separation. The stem cells are prepared into stem cell liposomes, and sanguinarine and curcumin are embedded in a composite shell material of chitosan and β-cyclodextrin to prepare sustained-release microspheres. The composition of mesenchymal stem cells derived from autologous fat prepared by the present invention has good air permeability, softness, certain mechanical properties and good biocompatibility, can rapidly promote wound healing, is convenient to remove, and will not cause secondary damage.

Some embodiments of the methods and compositions provided herein relate to the use of hapten labeled cells to stimulate chimeric antigen receptor (CAR) T cells. In some embodiments, CAR T cells can include a CAR that specifically binds to a hapten. Some embodiments relate to the in vivo or in vitro stimulation CAR T cells by hapten labeled cells.

The present invention discloses an immune cell expressing non-secretory IL-10 for enhancing cellular immunotherapy, a preparation method and a use thereof. The present invention unexpectedly discovered that non-secretory IL-10 can significantly reduce the mitochondrial dysfunction of CAR cells compared to secretory IL-10, and when facing target cell stimulation, compared with secretory IL-10, non-secretory IL-10 can effectively improve the viability of CAR cells and reduce the occurrence of CAR apoptosis. Based on this, the present invention expresses non-secretory IL-10 in CAR cells, while effectively reducing the main toxic and side effects of CAR cell therapy, by protecting the mitochondrial function of CAR cells and improving the viability of CAR cells, reducing the occurrence of CAR cell apoptosis and significantly improving the tumor killing ability of CAR cells, thereby achieving the attenuation and synergistic effect of CAR cells.

A therapeutic device for cell stimulation or cell therapy comprises a housing accommodating electrodes, a generator for generating high-frequency pulses, a processor unit comprising a control, regulation and calculation module, a memory unit, a control element, a controllable modulator by means of which the generator can be controlled. A voltage pulse sequence comprising a plurality of voltage pulses can be generated by means of the modulator, wherein the frequency of the voltage pulse sequence can be at least partially non-constant.

Disclosed herein include methods, compositions, and kits suitable for use in spatiotemporal regulation of therapeutic T-cells through a combination of molecular and physical actuation. There are provided, in some embodiments, thermal bioswitches that allow T-cells to sense small changes in temperature and use them as inputs for the actuation of genetic circuits. Also disclosed herein are T cell activity sensors. Genetic circuits capable of inducing expression of a payload upon thermal stimulation and / or immune cell stimulation are provided. There are provided, in some embodiments, thermally actuated immune cells and methods of using are provided. Oscillator circuits and methods of preventing T cell exhaustion are also disclosed.

The present disclosure provides cell stimulating particles and methods of using the same. Also provided are populations of cells produced using the cell-stimulating particles.

The utility model relates to the technical field of culture tubes, and discloses a quickly detachable modular triple cell stimulation culture tube which comprises a culture tube, the top of the culture tube is open, and a limiting groove is formed in one side of the top of the culture tube. According to the technical scheme, the inserting block in the pipe cap is inserted into the culture pipe, meanwhile, the inclined opening in the bottom of the inserting block continuously extrudes the wedge-shaped block in the positioning mechanism, and the wedge-shaped block is supported by the spring on the piston rod and continuously extrudes the wedge-shaped block along with the bottom of the inserting block; the wedge-shaped block can be stored in the limiting groove in the direction of the limiting block sliding block, when the wedge-shaped block corresponds to the clamping groove in the middle of the inserting block, the wedge-shaped block can be extruded out by the supporting force of the spring, the wedge-shaped block is limited in the clamping groove, the pipe cap is positioned, it is guaranteed that after an operator inserts the pipe cap to the top of the culture pipe, the culture pipe can be rapidly sealed, and the working efficiency is improved. And the pipe cap is locked through the positioning mechanism, so that the pipe cap is effectively prevented from being opened under the condition of external force.

The application discloses a cell treatment method based on sinusoidal alternating current stimulation, and the method comprises the following steps: applying sinusoidal alternating current stimulation to target stem cells, and the mitochondrial membrane potential, ATP synthesis and aerobic respiration level of the stem cells treated by the electric stimulation are improved. The application further discloses a cell stimulation device based on sinusoidal alternating current stimulation, and the device comprises a cell culture container and a planar interdigital electrode substrate placed in the cell culture container, wherein the planar interdigital electrode substrate comprises an optically transparent substrate and metal planar interdigital electrodes on the substrate. The application discloses the application of the stem cells treated by the above method or the stem cells treated by the device in the preparation of a medicine for mitochondrial transplantation treatment. The application improves the mitochondrial membrane potential, ATP synthesis capacity and aerobic respiration level of the cells by applying sinusoidal alternating current stimulation to the target stem cells.

The present invention provides a culture medium for inducing type C1 iNKT cells, a culture method thereof and applications thereof, relating to the technical field of cell culture. The culture medium comprises a DC culture medium and an iNKT culture medium. The DC culture medium comprises GM-CSF, IL-4, TNF-α, LPS, 7DW8-5 and autologous plasma. The iNKT culture medium comprises IL-2, IL-7, IL-15, IL-21, 7DW8-5, lupeol, betulinic acid, resveratrol and autologous plasma. Meanwhile, a culture method corresponding to the culture medium is also provided. The type C1 iNKT cells obtained after being cultured with the culture medium of the present invention for 14 days can secrete a relatively high level of IFN-γ without the stimulation of target cells, and the proportion of type C1 iNKT cells in CD3+ T cells increases significantly. Such a culture medium and a culture method can be used in the fields of inducing the growth and expansion of type C1 iNKT cells, exosome extraction, etc., so as to efficiently obtain target cells and target factors.

In an embodiment, the present disclosure pertains to an organ-chip model having a plurality of cell culture chambers connected through arrays of microfluidic channels. In some embodiments, each cell culture chamber of the plurality of cell culture chambers include an inlet and an outlet. In some embodiments, the inlet is configured to receive at least one of a cell, cell media, or a cell stimulant. In some embodiments, at least one outlet is configured to collect effluent. In some embodiments, the organ-chip model can include, without limitation, an organ-chip model of amnion membrane, an organ-chip model of a feto-maternal interface (fetal membrane-decidua parietalis), an organ-chip model of a feto-maternal interface (placenta-decidua interface), an organ-chip model of a cervix, and combinations thereof. In some embodiments, the organ-chip model is an interconnected organ-chip model having a combination of one or more organ-chip models with interconnected cell culture chambers.

An exemplary pulsed electric field system and method that can uniformly emit electrical pulses via use of a conductivity-controlled liquid medium to penetrate a pre-defined depth of a target tissue for a whole or region of a hollow or tubular organ to induce a tissue response. The conductivity-controlled liquid medium allows more uniform electrical pulses to be generated with less localized or focal bursts of pulses, which can reduce the required power rating of the electric generator for the pulses. The pulsed electric field system and method can be used for irreversible electroporation and subsequent exfoliation of the surface tissue, electroporation, pulsed field ablation, or cell stimulation.

Provided is a chimeric antigen receptor targeting BCMA. The receptor comprises a humanized scFv structure targeting BCMA, wherein the heavy-chain variable region scFv-VH and the light-chain variable region scFv-VL of scFv are connected via one or more G4S sequences. A new second-generation CAR-T cell targeting BCMA is successfully constructed by means of transduction by using a retroviral vector. In vitro and in vivo experiments show that after stimulation with BCMA positive tumor cells, the BCMA CAR 31-T cells can be effectively activated to secrete cytokines such as IFN-γ and TNF-α, thereby promoting the apoptosis of tumor cells. The BCMA CAR 31-T cells can effectively and specifically kill BCMA positive tumor cells.

The present invention describes a whole blood assay chip that enables separation of the cellular compartment from surrounding plasma, facilitating multiplexed measurement of otherwise difficult to detect soluble factors produced upon cellular stimulation. The invention is a microfluidic chip with an incubation chamber comprising an inlet, an outlet, and a fluidic barrier. Using this platform, the cellular compartment can be stimulated with various substances and levels of immunologic biomarkers could be measured. Through a proprietary immunologic algorithm described herein, this would allow for the diagnosis of an allergic response to foods, antibiotics, and / or other drugs. Additional applications of the invention besides allergy testing could include pregnancy testing, blood type, and medical applications requiring whole blood cellular stimulation readouts.

A fusion protein of IL-21 cytokine and IL-21 receptor is disclosed. Also disclosed are cells comprising the cytokine-receptor fusion, and methods and compositions for the treatment of tumors utilizing the cytokine-receptor fusion and utilizing the cells expressing the cytokine-receptor fusion.

The invention discloses a tumor antigen screening method based on HLA (human leukocyte antigen) typing. According to the method disclosed by the invention, on the basis of biosignal screening, antigen presentation and T cell stimulation immune screening are carried out through human body antigen presenting cells (APC). The immortalized B cells are used as antigen presenting cells and are long-acting and stable, APC cells of different HLA types are respectively set for antigen screening by referring to high-frequency HLA type arrangement characteristics, the coverage is wide, and the specificity is high; according to the method disclosed by the invention, rapid antigen screening can be carried out on a high-frequency antigen of a population queue or a personalized antigen of a single sample, and the high-immunogenicity antigen screened by the method disclosed by the invention can be used for tumor vaccine or TCR-T construction, so that tumor neoantigen screening can be carried out more rapidly and accurately by the method disclosed by the invention; the screened target spot can be used as a tumor treatment target spot applied to tumor vaccines, CAR-T or TCR-T cell therapy and the like.

The application relates to the technical field of microfluidic chip, and particularly discloses an ITO glass digital microfluidic system based on an upper pressing probe array. The system comprises an ITO electrode array module, a probe array module, a mobile functional probe module and a control system, the non-permanent connection of the ITO electrode is realized by adopting the upper pressing spring probe, the wiring on the surface of the substrate is reduced, and the optical transparency is improved; two sets of independent but cooperatively working electrode systems are adopted, and a double-mode control system based on an independent double-path DAC module + high-voltage operational amplifier provided by FPGA is used to realize droplet driving and cell stimulation respectively, the decoupling and integration of the driving and stimulation functions on the same platform are realized, the whole process automation of positioning, driving and stimulation is realized, and the flexibility and operation precision of the system are improved.

This invention relates to the use of a combination of peripheral blood concentrated cells and concentrated supernatant for the treatment of premature ovarian failure. In one aspect, the combination comprises peripheral blood concentrated cells, granulocyte-macrophage stimulating factors, a supernatant concentrate, and optional excipients; the peripheral blood concentrated cells and supernatant concentrate are prepared using an automated cell separation system and a tangential flow ultrafiltration system. The proportion of concentrated cells is such that the concentrated cells are CD45... + Cell count 4 x 10^6 cells: 10–15 ng granulocyte-macrophage stimulating factor: 75–125 μL supernatant concentrate. The invention also relates to a method for preparing the combination of the claims, comprising the step of mixing a specified amount of concentrated cells, granulocyte-macrophage stimulating factor, supernatant concentrate, and optional excipients to prepare a sterile formulation. The cell combination of the present invention exhibits excellent effects.

A cell stimulation apparatus according to an embodiment of the present invention includes: an electrode unit in which an electrode element is installed; a pMUT element disposed above the electrode unit to be spaced apart from the electrode unit, and configured to generate an ultrasonic wave through a voltage applied thereto; and a well element installed between the electrode unit and the pMUT element and forming a cell chamber together with the electrode unit and the pMUT element, wherein a bio-sample is disposed on the electrode element, and the ultrasonic wave generated by the pMUT element is transmitted to the bio-sample so as to provide ultrasound stimulation to the bio-sample.

The invention relates to an over-activated dendritic cell which can achieve durable anti-tumor immunity based on adoptive cell metastasis. The present application relates to cancer immunotherapies, such as T cell stimulation mediated anti-tumor therapies.

Provided herein are synthetic promoters, including synthetic transcriptional regulatory sequences and variant MND promoters, that can be used for controlling expression of transcribable sequences, such as chimeric antigen receptors (CARs) and T cell receptors (TCRs), such as by allowing for induction, repression, or constitutive activation of transcription of the transcribable sequence by T cell stimulation. Also provided herein are polynucleotides and vectors containing the synthetic transcriptional regulatory sequences and variant MND promoters and transcribable sequences described herein. Also provided herein are engineered T cells containing the synthetic transcriptional regulatory sequences, variant MND promoters, polynucleotides, and vectors described herein, as well as methods of making and using same.