70 results about "Cell stimulation" patented technology

The present invention relates generally to methods for stimulating cells, and more particularly, to a novel method to concentrate and stimulate cells that maximizes stimulation and / or proliferation of such cells. In the various embodiments, cells are stimulated and concentrated with a surface yielding enhanced proliferation, cell signal transduction, and / or cell surface moiety aggregation. In certain aspects methods for stimulating a population of cells such as T-cells, by simultaneous concentration and cell surface moiety ligation are provided by contacting the population of cells with a surface, that has attached thereto one or more agents that ligate a cell surface moiety and applying a force that predominantly drives cell concentration and cell surface moiety ligation, thereby inducing cell stimulation, cell surface moiety aggregation, and / or receptor signaling enhancement. Also provided are methods for producing phenotypically tailored cells, including T-cells for the use in diagnostics, drug discovery, and the treatment of a variety of indications, including cancer, viral infection, and immune related disorders. Compositions of cells having specific phenotypic properties produced by these processes are further provided.

The present invention relates generally to methods for stimulating cells, and more particularly, to a novel method to concentrate and / or stimulate cells that maximizes stimulation and / or proliferation of such cells. In the various embodiments, cells are stimulated and concentrated with a surface yielding enhanced proliferation, cell signal transduction, and / or cell surface moiety aggregation. In certain aspects methods for stimulating a population of cells such as T-cells, by simultaneous concentration and cell surface moiety ligation are provided by contacting the population of cells with a surface, that has attached thereto one or more agents that ligate a cell surface moiety and applying a force that predominantly drives cell concentration and cell surface moiety ligation, thereby inducing cell stimulation, cell surface moiety aggregation, and / or receptor signaling enhancement. Also provided are methods for producing phenotypically tailored cells, including T-cells for the use in diagnostics, drug discovery, and the treatment of a variety of indications, including cancer, viral infection, and immune related disorders. Compositions of cells having specific phenotypic properties produced by these processes are further provided.

The present invention provides methods and devices for the knowledge-based discovery and optimization of differences between cell types. In particular, the present invention provides visual servoing optical microscopy, as well as analysis methods. The present invention provides means for the close monitoring of hundreds of individual, living cells over time: quantification of dynamic physiological responses in multiple channels; real-time digital image segmentation and analysis; intelligent, repetitive computer-applied cell stress and cell stimulation; and the ability to return to the same field of cells for long-term studies and observation. The present invention further provides means to optimize culture conditions for specific subpopulations of cells.

An improved electrode for neural stimulation, including deep brain stimulation, cortical stimulation, muscle stimulation and other similar applications. The improvement of our invention over prior art consisting of the possibility of a larger number of electrode pads from where to originate the electrical stimulation, thereby offering the possibility of fine control of the location of the stimulating signal. Our invention discloses a system of address wires which controls switches and demultiplexers to select one of a plurality of wires and one of a plurality of electrode pads from where the electric stimulation starts, and latches that maintain some selected choices after the address buses go on to select other wires and other electrode tips. Our invention also discloses time delay lines which are used to keep the stimulating pulses for a pre-assigned time, after which the stimulating pulses stop until further instruction to start again.

The present invention provides methods and devices for the knowledge-based discovery and optimization of differences between cell types. In particular, the present invention provides visual servoing optical microscopy, as well as analysis methods. The present invention provides means for the close monitoring of hundreds of individual, living cells over time; quantification of dynamic physiological responses in multiple channels; real-time digital image segmentation and analysis; intelligent, repetitive computer-applied cell stress and cell stimulation; and the ability to return to the same field of cells for long-term studies and observation. The present invention further provides means to optimize culture conditions for specific subpopulations of cells.

The invention discloses an integrated microfluidic chip for screening medicine. The integrated microfluidic chip comprises a PDMS (polydimethylsiloxane) fluid channel layer and a glass layer. The PDMS fluid channel layer and the glass layer are bonded with each other by oxygen plasma to form a fluid channel unit which is of an irreversible structure; the fluid channel unit comprises a concentration gradient generation structural region, an array cell culture region and a buffer structural region. The invention further provides a method for applying the integrated microfluidic chip. The integrated microfluidic chip and the method have the advantages that medicine concentration gradient generation, chip cell culture, medicine stimulation on cells, result generation and detection are integrated on the chip, the buffer structural region can be opened and closed, accordingly, the concentration gradient generation structural region and the array cell culture region can independently work and integrally run, and various functions can be flexibly switched over; cell inoculation, stimulated, washing and dyeing operation procedures and the like can be simplified by the aid of the method, various experimental correlation parameters can be obtained by means of one-step running, and a novel technical platform and the novel method can be provided for medicine screening and cell-medicine research.

The present invention provides methods for stimulating cells using quantum dots. In addition, the present invention provides methods for treating a variety of clinical conditions using stimulation of quantum dots to induce cell stimulation and / or function.

The present invention provides methods and devices for the knowledge-based discovery and optimization of differences between cell types. In particular, the present invention provides visual servoing optical microscopy, as well as analysis methods. The present invention provides means for the close monitoring of hundreds of individual, living cells over time: quantification of dynamic physiological responses in multiple channels; real-time digital image segmentation and analysis; intelligent, repetitive computer-applied cell stress and cell stimulation; and the ability to return to the same field of cells for long-term studies and observation. The present invention further provides means to optimize culture conditions for specific subpopulations of cells.

The invention relates to a CD19-based chimeric antigen receptor and application thereof, in particular to a lentivirus vector material built by a chimeric antigen receptor T (CAR-T) cell technology using a tumor specific target point CD19 as the basis, a method, and application thereof to anti-tumor treatment. The chimeric antigen receptor is formed by serially connecting an antigen combination structure domain, a membrane spaning structure domain, a costimulatory signal conduction region, a CD3 zeta signal conduction structure domain and an inducible suicide fusion structure domain, wherein the antigen combination structure domain is combined with the tumor surface antigen; the tumor surface antigen is CD19. The chimeric antigen receptor is subjected to specific gene transformation on theT cell stimulation signals. Compared with other chimeric antigen receptors, the chimeric antigen receptor provided by the invention has a better reaction effect and higher safety, so that the CAR-T cells have higher immune effects and low side effects; the treatment effect and safety of the CAR-T cells are enhanced.

The invention relates to a co-culture method of a DC cell and a CIK cell and belongs to the technical field of cell culture. The co-culture method comprises the following steps: 1) extracting a single nucleus cell; 2) culturing the CIK cell; 3) culturing the DC cell; 4) carrying out mixed culturing on the DC cell and the CIK cell; and 5) carrying out multiplication culture. According to the co-culture method disclosed by the invention, after the DC cell is stimulated to be mature and co-cultured with the CIK cell, a factor GM-CSF is added according to the concentration and replenished after culture for 12h, so that the activity of the DC cell is kept, the survival time of the DC cell in a CIK culture system is prolonged, and the activity of the DC cell is kept for a long time.

Piezoelectric nanotransducers for use in an in vivo treatment of cell stimulation through electrical stimulation are described. The nanotransducers are localized in a target site, and an electrical stimulus is induced in the same site through external stimulation of the nanotransducers by ultrasonic waves.

The invention discloses a sample pretreatment method based on flow combination ICP-MS (Inductively Coupled Plasma Mass Spectrometry) single cell protein detection, and belongs to the technical field of sample pretreatment of flow cytometry. The sample pretreatment method comprises the following steps: (1) collecting and transporting a whole blood sample; (2) performing PBMC (Peripheral Blood Mononuclear Cell) cell separation; (3) performing cell active dyeing; (4) performing cell stimulation; (5) performing cell immobilization; (6) performing surface antibody dyeing; (7) performing intracellular phosphorylated protein dyeing; (8) performing single cell labeling, and the like. With the combination of a metal element labeled antibody with cell surface antigen, labeled cells are mixed with beads as internal reference for standardization, dead cells and living cells are distinguished in the pretreatment process, then the situation that the testing result analysis and description are affected by too many dead cells is avoided, single cells are distinguished from cell dimmers, cell trimers or even cell multimers, and flow combination ICP-MS single cell protein detection requirements can be very well met.

The invention relates to an injection capable of treating androgenetic alopecia with dermal papilla cell exosomes. The injection is a normal saline solvent containing dermal papilla cell exosomes. A preparation method of the injection comprises fiver steps including dermal papilla cell separation, dermal papilla cell adherent culture, dermal papilla cell stimulation induced culture, exosome freezedried powder preparation, and injection preparation. The amount of the exosomes secreted by the dermal papilla cells prepared using the preparation method is large, the content of effective components capable of promoting hair regeneration is high, and the treatment effect of the injection on androgenetic alopecia is excellent.

A method for stimulating pancreatic cells to synthesize and / or excrete insulin comprises contacting insulin-producing cells, in particular their aqueous suspension, with a water-soluble cellulose derivative, in particular selected from alkylated, hydroxyalkylated, and alkylated-hydroxyalkylated cellulose or a mixture thereof. A medium for the culture of pancreatic beta-cells contains an effective cell-stimulating amount of a cellulose derivative. It can be used to stimulate pancreatic beta-cells to produce and / or excrete insulin. An apparatus for such stimulation comprises a container holding a solution of a cellulose derivative in an aqueous culture medium. Stimulation of pancreatic beta-cells by a cellulose derivative is useful in the management of diabetes. Further preservative or therapeutic methods using the aqueous cellulose derivatives are disclosed.

The present invention provides compositions and methods for modulating the growth, development, and repair of bone, cartilage, or other connective tissue. Also provided are devices and stimulation waveforms to differentially modulate the behavior of osteoblasts, chondrocytes, and other connective tissue cells to promote proliferation, differentiation, matrix formation, or mineralization for in vitro or in vivo applications. Continuous mode and pulsed segment mode of cell stimulation with charge-balanced signals can be used. Bone, cartilage, and other connective tissue growth is stimulated in part by the release of nitric oxide via electrical stimulation, and can be regulated by simultaneous supply of NO donors and NO synthase inhibitors. Bone, cartilage and other connective tissue growth is stimulated in part by the release of BMP-2 and BMP-7 in response to electrical stimulation that promotes cell differentiation. The methods and devices are useful for promoting bone fracture repair, cartilage and connective tissue repair, and for engineering tissue for transplantation.

The invention belongs to the field of a medical instrument, and provides a ultrasonic microbubble cell stimulation device and an operation method thereof. The ultrasonic microbubble cell stimulation device comprises: a culture dish, a positioning frame, and a base, a culture hole is arranged in the culture dish, a sealing film is arranged on an open end, a culture cup is arranged in the culture hole, and the base is connected with a transmission componentthat is for transmitting an ultrasonic wave to stimulate a cell and is capable of adjusting a distance from the base to the culture dish; andthe base and the positioning frame are connected through a position-adjusting component. The operation method of the ultrasonic microbubble cell stimulation device comprises: a guide rod installationstep; a transmission component connecting and assembly step; a positioning frame installation step; a culture cup installation and microbubble injecting step; and a culture dish installation step. The ultrasonic microbubble cell stimulation device can successfully prevent air bubbles from entering into the culture dish, ensuring that the ultrasonic waves act on the microbubbles directly, has a controllable stimulation area and intensity, is easy to operate, is versatile, and has a wide range of application.

The invention discloses a high-throughput microfluidic chip and a cell analysis device and method. The high-throughput microfluidic chip comprises a cell observation pool, a stimulant channel, a cell introduction channel, a buffer solution channel and a waste liquid channel, wherein the stimulant channel is connected with the opening of the cell observation pool, the cell introduction channel is connected with the side face of the cell observation pool, the connecting site is close to the opening, the buffer solution channel is connected with the bottom of the cell observation pool, and the waste liquid channel is connected with the opening of the cell observation pool. The cell analysis device comprises the high-throughput microfluidic chip, a signal collection device and electric proportional switching valves, and the channel inlet end of the high-throughput microfluidic chip is connected with independent electric proportional switching valves; the signal collection device is arranged at the cell observation pool and is used for collecting cell signals or imaging cells. The analysis method comprises the following steps: capturing cells, stimulating cells and releasing cells. The chip, the device and the method provided by the invention can be used for achieving high-throughput cell stimulation and analysis in multiple modes.

A porous structure (1) provided with a pattern that is composed of a conductive polymer, which comprises a porous body (2) and a pattern (3) that is composed of a conductive polymer and arranged on the porous body (2). The porous body (2) is preferably a gel, and a dopant may be added to the pattern (3) that is composed of a conductive polymer. If an agarose gel is used as the gel (2) and a PEDOT electrode (3A) is used as the pattern (3) that is composed of a conductive polymer in the porous structure (1) which is provided with the pattern (3) that is composed of a conductive polymer, the porous structure (1) can be used as an electrode for cell stimulation. The porous structure (1) provided with the pattern (3) that is composed of a conductive polymer can be produced by an electropolymerization method.

The invention relates to specifically relates to a genetically engineered cell and a method for efficiently amplifying NK cells in vitro by using the genetically engineered cell, belonging to the field of immunology. According to the invention, a Piggybac transposon system is utilized to construct a K562 engineered cell that realizes transmembrane stable expression of IL-15, IL-18 and 4-1BBL; andthe method for amplifying and activating NK cells based on the genetically engineered K562 cell is provided in virtue of an in-vitro cell stimulation technology. The genetically engineered cell can realize simultaneous transmembrane high-expression of IL-15, IL-18 and 4-1BBL. The NK cell amplification method of the invention is simple to operate and low in cost; the amplified NK cells have the advantages of high purity, large quantity, good killing activity, etc.; and the NK cell amplification method is suitable for large-scale production of NK cells and lays a good foundation for the clinicalapplication of adoptive immunotherapy of NK cells.

The present invention provides methods and devices for the knowledge-based discovery and optimization of differences between cell types. In particular, the present invention provides visual servoing optical microscopy, as well as analysis methods. The present invention provides means for the close monitoring of hundreds of individual, living cells over time; quantification of dynamic physiological responses in multiple channels; real-time digital image segmentation and analysis; intelligent, repetitive computer-applied cell stress and cell stimulation; and the ability to return to the same field of cells for long-term studies and observation. The present invention further provides means to optimize culture conditions for specific subpopulations of cells.

The present invention relates generally to methods for stimulating, activating, and maintaining or increasing the polyclonality of expressed TCRs in a population of T cells. In the various embodiments, cells are stimulated with a surface, wherein the surface has attached thereto one or more agents that ligate a cell surface moiety of at least a portion of the T cells and stimulates at least a portion of the T cells, yielding enhanced proliferation, cell signal transduction, and / or cell surface moiety aggregation. In certain aspects methods for stimulating a population of cells such as T-cells, by cell surface moiety ligation are provided by contacting the population of cells with a surface, that has attached thereto one or more agents that ligate a cell surface moiety thereby inducing cell stimulation, cell surface moiety aggregation, and / or receptor signaling enhancement. Also provided are methods for producing T-cells for the use in diagnostics and the treatment of a variety of indications, including cancer, viral infection, and immune related disorders. Compositions of cells having increased polyclonality produced by these processes are further provided.

A device for electrically stimulating living cells and promoting biological cell growth and differentiation is hereby disclosed, said device being based on the generation of an electrical field due to a piezoelectric effect yielded by a nanostructure made from a piezoelectric material. When a cell contacts the nanostructure, the nanostructure suffers a mechanical stress which in turn produces an electrical field that locally stimulates the cell membrane. This in-situ electrical stimulation allows the activation of voltage-dependent ionic channels present in the membrane of electroconductive cells. It allows the control of key cellular messengers, such as calcium ions, that can lead to the stimulation of neural circuits in neurons, provoking motion in muscle cells or promoting cell growth and differentiation.

Provided herein is are methods for evaluating the biological activity and / or therapeutic potential of a glucan, comprising in one embodiment: co-culturing a first population of cells with a second population of cells, wherein the first population comprises cells capable of being stimulated by said glucan to produce and / or secrete cytokines and growth factors, and the second population comprises collagen-producing cells; contacting said co-cultured cells with the glucan and incubating for a period of time sufficient to induce the production of collagen from the collagen-producing cells; and determining the level of production of collagen from said collagen-producing cells, wherein the level of production of collagen is indicative of the biological activity and / or therapeutic potential of the glucan.

The present invention relates to the field of therapeutic immunization, specifically with the use of a novel hepatitis B virus (HBV) antigen formulation for cell stimulation. The formulation is formed by HBV surface antigen (HBsAg) and nucleocapsid antigen (HBcAg) precipitates in suspension. The formulation contains said antigens as precipitates in suspension as particles with sizes less than 500 nm and greater than 500 nm, in a mixture in which the proportion between the particles of the sizes mentioned is between 50% - 50% and 80% - 20%, respectively. The selection of a range of particle sizes allows the levels of stimulation of various cell types to be maximized. In addition, a description is given of a method for cell stimulation using said formulation and the subsequent passive immunization of patients with chronic hepatitis B, based on the maximum in vivo or in vitro stimulation of heterologous or autologous cells (dendritic cells, B cells and macrophages). The cells stimulated with this formulation are transferred to patients with a chronic HBV infection.

Type 2 diabetes (T2D) is a clinical syndrome caused by insufficient insulin secretion for insulin requirements. described herein are compositions and methods for microphysiological MPS models of disease (MODs) for diabetes. These platforms allow one to compare the effect of chronic β-cell stimulation in the presence and absence of patient specific immune cells in IPSC-derived islets from each group. Additionally, one can reproduce the T2D β-cell phenotype, using islets-on-chips will also be exposed to gluco-lipotoxicity. Likewise, skeletal muscle-on-chips are exposed to patient specific activated immune cells, variable motor neuron innervation and lipids characteristic of T2D.

We describe an improved method for generating sizable numbers of mature dendritic cells from nonproliferating progenitors in human blood. The first step or “priming” phase is a culture of T cell depleted mononuclear cells in medium supplemented with GM-CSF and IL-4 to produce immature dendritic cells. The second step or “differentiation” phase requires the exposure to dendritic cell maturation factor such as monocyte conditioned medium. Using this two-step approach, substantial yields are obtained. The dendritic cells derive from this method have all the features of mature cells. They include a stellate cell shape, nonadherence to plastic, and very strong T cell stimulatory activity. The mature dendritic cells produced according to this invention are useful for activating T cells.

The invention discloses a cell addressing micro-fluidic chip, a cell analysis device and a cell analysis method. The chip includes a cell culture channel, a pair of side pressure channels, an addressing channel, a stimulation channel and waste liquid channels. During work, when the chip is in an addressing state, the pressure of the addressing channel is greater than that of the stimulation channel; when the chip is in a stimulated state, the pressure of the stimulation channel is greater than that of the addressing channel. The cell analysis device comprises the micro-fluidic chip, a signal acquisition device and electric proportioning conversion valves, wherein the inlet ends of the side pressure channels, the addressing channel and the stimulation channel of the chip are respectively connected with the independent electric proportioning conversion valves; each electric proportioning conversion valve outputs corresponding hydraulic pressure according to different electrical signals; the signal acquisition device is arranged in the cell culture channel and used for collecting cell signals or imaging cells. According to the invention, the technical problems such as solution switching time, cellular environment control and stimulating point space control in an existing micro-fluidic based cell stimulation method are solved.

Cell stimulation, staining, and visualization are common techniques in both clinical and research settings. The invention is directed to microfluidic devices for in situ cell stimulation, staining, and / or visualization, and related methods for applying one or more stimuli to the cells, and fixing and staining of cells in situ. The device allows for high-throughput screening of living cells using a minimal quantity of reagents where the fate of individual cells can be followed over time.

The invention is applicable to the technical field of cell stimulation, and provides a cell stimulation device and a cell stimulation method. The cell stimulation device includes stimulation probes, abase, and a positioning base that can slide relative to the base. The base is provided with a plurality of holes for mounting the stimulation probes, the positioning base is provided with a positioning structure for positioning a culture dish, and the stimulation probes are mounted at the holes and towards the positioning base. The invention provides the cell stimulation device and the cell stimulation method, cells can be stimulated by controlling the area and intensity of uniform ultrasonic stimulation on the cells in the culture dish through cooperation with arrays of the stimulation probes in different size and height adjustment of the positioning base, the experiment effect is good, operation is accurate, simple and convenient, stimulation of multiple groups of cells at the same timecan be achieved and the uniformity is good.