High-throughput microfluidic chip and cell analysis device and method

A microfluidic chip and analysis device technology, applied in the field of medical devices, can solve the problems of low throughput and complicated high-throughput cell collection process, and achieve the effect of simple structure and controllable stimulation time
CN104498327AActive Publication Date: 2015-04-08HUAZHONG UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Applications(China)
Current Assignee / Owner
HUAZHONG UNIV OF SCI & TECH
Publication Date
2015-04-08

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Abstract

The invention discloses a high-throughput microfluidic chip and a cell analysis device and method. The high-throughput microfluidic chip comprises a cell observation pool, a stimulant channel, a cell introduction channel, a buffer solution channel and a waste liquid channel, wherein the stimulant channel is connected with the opening of the cell observation pool, the cell introduction channel is connected with the side face of the cell observation pool, the connecting site is close to the opening, the buffer solution channel is connected with the bottom of the cell observation pool, and the waste liquid channel is connected with the opening of the cell observation pool. The cell analysis device comprises the high-throughput microfluidic chip, a signal collection device and electric proportional switching valves, and the channel inlet end of the high-throughput microfluidic chip is connected with independent electric proportional switching valves; the signal collection device is arranged at the cell observation pool and is used for collecting cell signals or imaging cells. The analysis method comprises the following steps: capturing cells, stimulating cells and releasing cells. The chip, the device and the method provided by the invention can be used for achieving high-throughput cell stimulation and analysis in multiple modes.
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Description

technical field

[0001] The invention belongs to the field of medical devices, and more specifically relates to a high-throughput microfluidic chip, a cell analysis device and a method. Background technique

[0002] Cells are the basic unit of all known life in nature except viruses, and the basis of all life activities. Therefore, in-depth research on cells is the key to uncovering the mysteries of life and transforming life. The ability of cells to recognize and respond to their microenvironment through signal transduction is crucial for the development, tissue repair, and immunity of organisms. When cells are stimulated by different chemical drugs, they will trigger the spatiotemporal changes of cell response, regulate the electrophysiological activity of cells, thereby affecting the growth and differentiation of cells and maintaining the physiological balance of cells.

[0003] Many dynamic intracellular reactions, such as protein transport, protein interaction, post-tra...

Claims

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