645 results about "Cells signal" patented technology

The present invention relates generally to methods for stimulating cells, and more particularly, to a novel method to concentrate and stimulate cells that maximizes stimulation and/or proliferation of such cells. In the various embodiments, cells are stimulated and concentrated with a surface yielding enhanced proliferation, cell signal transduction, and/or cell surface moiety aggregation. In certain aspects methods for stimulating a population of cells such as T-cells, by simultaneous concentration and cell surface moiety ligation are provided by contacting the population of cells with a surface, that has attached thereto one or more agents that ligate a cell surface moiety and applying a force that predominantly drives cell concentration and cell surface moiety ligation, thereby inducing cell stimulation, cell surface moiety aggregation, and/or receptor signaling enhancement. Also provided are methods for producing phenotypically tailored cells, including T-cells for the use in diagnostics, drug discovery, and the treatment of a variety of indications, including cancer, viral infection, and immune related disorders. Compositions of cells having specific phenotypic properties produced by these processes are further provided.

The present invention relates generally to methods for stimulating cells, and more particularly, to a novel method to concentrate and/or stimulate cells that maximizes stimulation and/or proliferation of such cells. In the various embodiments, cells are stimulated and concentrated with a surface yielding enhanced proliferation, cell signal transduction, and/or cell surface moiety aggregation. In certain aspects methods for stimulating a population of cells such as T-cells, by simultaneous concentration and cell surface moiety ligation are provided by contacting the population of cells with a surface, that has attached thereto one or more agents that ligate a cell surface moiety and applying a force that predominantly drives cell concentration and cell surface moiety ligation, thereby inducing cell stimulation, cell surface moiety aggregation, and/or receptor signaling enhancement. Also provided are methods for producing phenotypically tailored cells, including T-cells for the use in diagnostics, drug discovery, and the treatment of a variety of indications, including cancer, viral infection, and immune related disorders. Compositions of cells having specific phenotypic properties produced by these processes are further provided.

A device and a method of use for treating a medical disorder by surgically implanting into a patient at least one sensor element capable of detecting and conveying cell signals: attaching a management unit such that a micro controller of the management unit is connected to at least one sensor element; and connecting the management unit via a lead bundle to at least one treatment device. The treatment device may be an electrical stimulation device, a magnetic stimulation device, a heat transfer device, or a medication delivery device. Responsive to signals from the one or more sensor elements, mathematical algorithms of the management unit use wavelet crosscorrelation analysis to prompt delivery of at least one treatment modality, such heat transfer, current pulses, magnetic stimulation or medication. The medical disorder may arise from the brain, central nervous system or organs and tissues outside of the central nervous system.

A method for sensing a close to ground signal recieved from an array cell within a memory array includes the steps of providing a reference unit with a reference cell having a similar structure and a similar current path therethrough to that of the array cell, providing a timing unit with a timing cell having a similar structure and a similar current path therethrough to that of the array cell, discharging the array, the reference unit and the timing unit prior to charging them, generating a cell signal, a reference signal and a timing signal, respectively, upon charging, generating a read signal when the timing signal at least reaches a predefined voltage level and generating a sensing signal from the difference of the cell and reference signals once the read signal is generated. The reference unit has a reference capacitance which is a multiple of the expected capacitance of a bit line of the array and the timing unit has a predefined timing capacitance.

Methods, apparatus, media and signals for identifying associated cell signaling proteins are disclosed. The method involves producing and storing a comparison value for each pair of the cell signaling proteins in response to data values representing physical properties of respective cell signaling proteins. The method further involves identifying cell signaling protein pairs having comparison values satisfying a condition indicative of an association between the cell signaling proteins.

A method for conducting a broad range of biochemical analyses or manipulations on a series of nano- to subnanoliter reaction volumes and an apparatus for carrying out the same are disclosed. The invention is implemented on a fluidic microchip to provide high serial throughput. In particular, the disclosed device is a microfabricated channel device that can manipulate nanoliter or subnanoliter reaction volumes in a controlled manner to produce results at rates of 1 to 10 Hz per channel. The reaction volumes are manipulated in serial fashion analogous to a digital shift register. The invention has application to such problems as screening molecular or cellular targets using single beads from split-synthesis combinatorial libraries, screening single cells for RNA or protein expression, genetic diagnostic screening at the single cell level, or performing single cell signal transduction studies.

Isolated polypeptides comprising or consisting essentially of specific structural motifs (e.g., three β-sheets and two α-helices) are provided, wherein the polypeptides exhibit at least one cell signaling and/or other non-canonical activity of biological relevance. Also provided are polynucleotides encoding such polypeptides, binding agents that bind such polypeptides, analogs, variants and fragments of such polypeptides, etc., as well as compositions and methods of identifying and using any of the foregoing.

The invention provides a method and a device of transmitting and acquiring synchronous information blocks. The method comprises steps that according to a synchronous information block set SS burst Set mode, target mode index information is determined; the target mode index information is stored in every synchronous information block SS block, and the to-be-transmitted synchronous information block is acquired; by adopting a high frequency wave beam, user equipment in a target cell is periodically transmitted to the target synchronous information block; every SS burst Set comprises periodically transmitted synchronously burst SS burst of a first preset number; every SS burst comprises target synchronous information blocks of a second preset number are transmitted according to a space sequence. By adopting the method provided by the invention, the cell signal synchronizing of the user equipment in a 5G network is realized accurately and quickly.

Systems and methods for providing a framework for supporting a self organizing/optimization network (SON) feature in an over the air communications system. An enhanced mobile communications device is provided that performs functions to creating a framework for enhancing SON algorithms. The communications device stores event analysis data in an on board memory and, on certain conditions, signals the analysis to the network. The analysis includes information useful in performing SON algorithms at the network level, such as cell coverage and neighboring cell signal level information, cell selection and reselection information at different locations, relative signal strength between cell base stations at different locations, and coverage hole information. Utilizing the stored information, the network can automatically perform SON algorithms to improve network efficiency without the need for manual intervention by an operator. Embodiments include event filtering by the communications device to efficiently utilize the on board storage.

This invention is directed to a method for preparation of a biological sample for measurement of protein epitopes that allows for the preservation of intracellular protein epitopes and detection of signal transduction pathways based on the ability to capture transient activation states of the epitopes. The method provided by the invention allows for the rapid fixation of biological samples containing red blood cells, to ensure that epitopes of signal transduction molecules and other intracellular protein epitopes are preserved in the active state. The method of the invention further allows for lysis of red blood cells, thereby making it a useful method for cytometric analysis of biological samples, including, for example, whole blood, bone marrow aspirates, peritoneal fluids, and other red blood cell containing samples. The invention also provides a method to recover or “unmask” epitopes on intracellular antigens that have been made inaccessible by the cross linking fixative necessary to fix the sample. Significantly, the methods of the invention allow preservation and analysis of phospho-epitope levels in biological samples taken directly from patients to determine disease-specific characteristics.

The invention provides a chimeric antigen receptor (CAR) (a) an antigen binding domain of HN1 or SS, a transmembrane domain, and an intracellular T cell signaling domain, or (b) an antigen binding domain of SS1, a transmembrane domain, an intracellular T cell signaling domain, and a granulocyte-macrophage colony-stimulating factor (GM-CSF) receptor 2 leader. Nucleic acids, recombinant expression vectors, host cells, populations of cells, antibodies, or antigen binding portions thereof, and pharmaceutical compositions relating to the CARs are disclosed. Methods of detecting the presence of cancer in a mammal and methods of treating or preventing cancer in a mammal are also disclosed.

The present invention relates to novel polypeptides that bind to IL-23 receptor and inhibit the binding of IL-23 to its corresponding receptor and cell signaling thereof. The novel polypeptides of the present invention has a core structure of WX₁X₂X₃W,

where W is tryptophan, and X₁, X₂ and X₃ are amino acids, with the proviso that when one of X₁, X₂ or X₃ is W, the remaining two of X₁, X₂ or X₃ cannot be W. Preferred core structures include WVDYW or WQDYW. The present invention relates a composition containing the novel polypeptides, and use of same in treating IL-23 associated human diseases including, for example, inflammatory bowel diseases, psoriasis and Crohn's disease.

The invention discloses a gene control device and method based on CRISPR-Cas9. The device comprises a nucleotide sequence, wherein the nucleotide sequence is an sgRNA itself or can be transcribed into an sgRNA, the sgRNA structurally comprises a first part bonded with inactivated Cas9 protein and a second part connected to the 3'end of the first part, the 5'end of the first part is provided with an antisense sequence, and the second part is an aptamer sequence part which comprises a ligand binding part and an aptamer stem part; when no ligand exists, the aptamer sequence is pair-bonded with the aptamer stem part; when a ligand exists, the aptamer sequence is dissociated from the aptamer stem part so as to be pair-bonded with a target DNA. By integrating an RNA riboswitch bonded with a specific biological signal into the sgRNA, a bridge of cell signal intensity and gene expression intensity is established, any target gene can be activated or silenced on the basis that biological signal identification is achieved, and artificial connection is built between signal ligands and cytogene.

The present invention provides for chimeric Wnt antagonists comprising a Frz domain component derived from a Frizzled protein, a secreted Frizzled related protein or Ror protein and an Fc immunoglobulin component, and their use in the treatment and diagnostic detection of cellular Wnt signaling and Wnt-mediated disorders, including cancer.

The present invention provides high speed mobile terminal cell switchover control method. Said method includes when terminal moves away current service base station at higher set traveling speed and approaches predicted target cell, network side judges target cell signal whether satisfying pre-switching condition, if satisfying then starting up pre-switching and establishing channel in target cell. Said invention realizes high speed mobile terminal switching through predetermining mobile terminal travel direction, pre-applying channel resource in target cell and establishing channel.

The invention provides a determination method for a voice service scheme of a VoLTE (Voice over Long Term Evolution) terminal and the VoLTE terminal. The method comprises the following steps: reselecting from a 2G/3G (Two-Generation/Three-Generation) cell to an LTE cell by the VoLTE terminal according to cell signal intensity measured in a preset range; if a reselected LTE cell identity is stored at the VoLTE terminal, transmitting a first position updating request to a mobile management entity MME by the VoLTE terminal, wherein a VoLTE voice service scheme restriction identity is carried in the first position updating request, so an IMS (IP Multimedia Subsystem) unregistration request message of the VoLTE terminal is transmitted to an IMS by a home subscriber server HSS according to the VoLTE voice service scheme restriction identity carried in a first position updating message; receiving, by the VoLTE terminal, an IMS unregistration success response message transmitted by the IMS; determining circuit switched fallback CSFB according to the IMS unregistration success response message to serve as the voice service scheme of the VoLTE terminal to perform the voice service.

The invention relates to a culture medium for preparing neural stem cells and an application of the culture medium. The culture medium for preparing the neural stem cells comprises a culture medium suitable for the growth of stem cells, and a cell signal pathway inhibitor which is selected from at least one of the following agents: a GSK (Glaxo Smith Klein) inhibitor, an MEK (Methyl Ethyl Ketone) inhibitor, a TGF (Transforming Growth Factor)-beta inhibitor, a ROCK inhibitor and a BMP inhibitor. The somatic cells are cultured by using the culture medium, especially the somatic cells for expressing the transcription regulator are cultured, so as to effectively transdifferentiate the somatic cells into neutral stem cells and the transdifferentiation time is greatly shortened.

Signal Transducer and Activator of Transcription (STAT) proteins have a fundamental role cell signaling, and are activated by a large number of cytokines and growth factors. One member of the STAT family, STAT3, has a critical role in oncogenesis. The present invention relates generally to disruption of the pathway of STAT3 signaling in the treatment of human cancer. STAT3 activation is shown to be present in diverse tumor cell lines and tumors, to promote oncogenesis, to inhibit apoptosis, and to reduce sensitivity to chemotherapeutic agents. Inhibition of STAT3 signaling induces apoptosis specifically in tumor cell lines, and increases sensitivity to chemotherapeutic agents. The invention relates more particularly to methods, compositions, means of administering such compositions, and means for identifying such compositions for the inhibition of STAT3 intracellular signaling in the treatment of human cancers.