665 results about "Cells signal" patented technology

The present invention relates generally to methods for stimulating cells, and more particularly, to a novel method to concentrate and stimulate cells that maximizes stimulation and / or proliferation of such cells. In the various embodiments, cells are stimulated and concentrated with a surface yielding enhanced proliferation, cell signal transduction, and / or cell surface moiety aggregation. In certain aspects methods for stimulating a population of cells such as T-cells, by simultaneous concentration and cell surface moiety ligation are provided by contacting the population of cells with a surface, that has attached thereto one or more agents that ligate a cell surface moiety and applying a force that predominantly drives cell concentration and cell surface moiety ligation, thereby inducing cell stimulation, cell surface moiety aggregation, and / or receptor signaling enhancement. Also provided are methods for producing phenotypically tailored cells, including T-cells for the use in diagnostics, drug discovery, and the treatment of a variety of indications, including cancer, viral infection, and immune related disorders. Compositions of cells having specific phenotypic properties produced by these processes are further provided.

The present invention relates generally to methods for stimulating cells, and more particularly, to a novel method to concentrate and / or stimulate cells that maximizes stimulation and / or proliferation of such cells. In the various embodiments, cells are stimulated and concentrated with a surface yielding enhanced proliferation, cell signal transduction, and / or cell surface moiety aggregation. In certain aspects methods for stimulating a population of cells such as T-cells, by simultaneous concentration and cell surface moiety ligation are provided by contacting the population of cells with a surface, that has attached thereto one or more agents that ligate a cell surface moiety and applying a force that predominantly drives cell concentration and cell surface moiety ligation, thereby inducing cell stimulation, cell surface moiety aggregation, and / or receptor signaling enhancement. Also provided are methods for producing phenotypically tailored cells, including T-cells for the use in diagnostics, drug discovery, and the treatment of a variety of indications, including cancer, viral infection, and immune related disorders. Compositions of cells having specific phenotypic properties produced by these processes are further provided.

Provided are antibodies that specifically bind to Programmed Death-1 (PD1, Pdcd-1, or CD279) and inhibit PD1-mediated cellular signaling and activities in immune cells, antibodies binding to a set of amino acid residues required for its ligand binding, and uses of these antibodies to treat or diagnose cancer, infectious diseases or other pathological disorders modulated by PD1-mediated functions.

A device and a method of use for treating a medical disorder by surgically implanting into a patient at least one sensor element capable of detecting and conveying cell signals: attaching a management unit such that a micro controller of the management unit is connected to at least one sensor element; and connecting the management unit via a lead bundle to at least one treatment device. The treatment device may be an electrical stimulation device, a magnetic stimulation device, a heat transfer device, or a medication delivery device. Responsive to signals from the one or more sensor elements, mathematical algorithms of the management unit use wavelet crosscorrelation analysis to prompt delivery of at least one treatment modality, such heat transfer, current pulses, magnetic stimulation or medication. The medical disorder may arise from the brain, central nervous system or organs and tissues outside of the central nervous system.

Systems, methods and devices for restoring or enhancing one or more motor functions of a patient are disclosed. The system comprises a biological interface apparatus and a joint movement device such as an exoskeleton device or FES device. The biological interface apparatus includes a sensor that detects the multicellular signals and a processing unit for producing a control signal based on the multicellular signals. Data from the joint movement device is transmitted to the processing unit for determining a value of a configuration parameter of the system. Also disclosed is a joint movement device including a flexible structure for applying force to one or more patient joints, and controlled cables that produce the forces required.

A method for sensing a close to ground signal recieved from an array cell within a memory array includes the steps of providing a reference unit with a reference cell having a similar structure and a similar current path therethrough to that of the array cell, providing a timing unit with a timing cell having a similar structure and a similar current path therethrough to that of the array cell, discharging the array, the reference unit and the timing unit prior to charging them, generating a cell signal, a reference signal and a timing signal, respectively, upon charging, generating a read signal when the timing signal at least reaches a predefined voltage level and generating a sensing signal from the difference of the cell and reference signals once the read signal is generated. The reference unit has a reference capacitance which is a multiple of the expected capacitance of a bit line of the array and the timing unit has a predefined timing capacitance.

A device for the high-speed analysis of photon- or particle-generated image data or for the high-speed energy-discrimination analysis of photon- or particle-counting data. The device uses a sensor that collects the photons or particles on an array of solid state detectors, as electrical analog signals, and stores the analog-signal information on capacitors of readout arrays associated with the detector arrays. Integration of the photon or particle flux signals on the readout arrays proceeds for a given time frame and then image-related signals are transferred to an analog correction processor. The analog correction processor is comprised of one or more integrated circuit chips where each chip contains an array of correction processor unit cells. In these unit cells signals are corrected, in parallel, for gain and offset nonuniformities in the detection and processing chain. Corrections to all the signals are made in a time frame or less and the information is then transferred to an analog image processor. Particle-counting data is transferred directly from the readout array chips to the analog image processor. The analog image processor is comprised of one or more integrated circuit chips where each chip is made up of an array of image processor unit cells. Each unit cell contains circuitry for implementing an image processing or energy discrimination algorithm, and circuitry for outputting the signals and / or the position of only those unit cells for which the algorithm is satisfied. The analog image processor chip may also contain circuitry that counts the number of cells for which the algorithms have been satisfied. The analog image processor implements the algorithm and outputs the data in a time frame or less.

Methods and composition of induction of pluripotent stem cells are disclosed. For example, in certain aspects methods for generating essentially vector-free induced pluripotent stem cells with cell signaling regulators are described. Furthermore, certain aspects of the invention provide novel compositions comprising induced pluripotent stem cells essentially free of exogenous retroviral vector elements in the presence of a medium comprising signaling inhibitors. In certain aspects, feeder-free episomal reprogramming methods may be provided.

The disclosure provides a chimeric antigen receptor (CAR) comprising a) an antigen binding domain of HA22, a transmembrane domain, and an intracellular T cell signaling domain; or b) an antigen binding domain of BL22, a transmembrane domain, and an intracellular T cell signaling domain comprising CD28 and / or CD137. Nucleic acids, recombinant expression vectors, host cells, populations of cells, antibodies, or antigen binding portions thereof, and pharmaceutical compositions relating to the CARs are disclosed. Methods of detecting the presence of cancer in a mammal and methods of treating or preventing cancer in a mammal are also disclosed.

Methods, apparatus, media and signals for identifying associated cell signaling proteins are disclosed. The method involves producing and storing a comparison value for each pair of the cell signaling proteins in response to data values representing physical properties of respective cell signaling proteins. The method further involves identifying cell signaling protein pairs having comparison values satisfying a condition indicative of an association between the cell signaling proteins.

A device and method of use for treating a medical disorder by surgically implanting into a patient at least one sensor element capable of detecting and conveying cell signals; attaching a management unit such that a micro controller of the management unit is connected to at least one sensor element; and connecting the management unit via a lead bundle to at least one treatment device. The treatment device may be an electrical stimulation device, a magnetic stimulation device, a heat transfer device, or a medication delivery device. Responsive to signals from the one or more sensor elements, mathematical algorithms of the management unit use wavelet crosscorrelation analysis to prompt delivery of at least one treatment modality, such heat transfer, current pulses, magnetic stimulation or medication. The medical disorder may arise from the brain, central nervous system or organs and tissues outside of the central nervous system.

A method for conducting a broad range of biochemical analyses or manipulations on a series of nano- to subnanoliter reaction volumes and an apparatus for carrying out the same are disclosed. The invention is implemented on a fluidic microchip to provide high serial throughput. In particular, the disclosed device is a microfabricated channel device that can manipulate nanoliter or subnanoliter reaction volumes in a controlled manner to produce results at rates of 1 to 10 Hz per channel. The reaction volumes are manipulated in serial fashion analogous to a digital shift register. The invention has application to such problems as screening molecular or cellular targets using single beads from split-synthesis combinatorial libraries, screening single cells for RNA or protein expression, genetic diagnostic screening at the single cell level, or performing single cell signal transduction studies.

Isolated polypeptides comprising or consisting essentially of specific structural motifs (e.g., three β-sheets and two α-helices) are provided, wherein the polypeptides exhibit at least one cell signaling and / or other non-canonical activity of biological relevance. Also provided are polynucleotides encoding such polypeptides, binding agents that bind such polypeptides, analogs, variants and fragments of such polypeptides, etc., as well as compositions and methods of identifying and using any of the foregoing.

The invention provides a method and a device of transmitting and acquiring synchronous information blocks. The method comprises steps that according to a synchronous information block set SS burst Set mode, target mode index information is determined; the target mode index information is stored in every synchronous information block SS block, and the to-be-transmitted synchronous information block is acquired; by adopting a high frequency wave beam, user equipment in a target cell is periodically transmitted to the target synchronous information block; every SS burst Set comprises periodically transmitted synchronously burst SS burst of a first preset number; every SS burst comprises target synchronous information blocks of a second preset number are transmitted according to a space sequence. By adopting the method provided by the invention, the cell signal synchronizing of the user equipment in a 5G network is realized accurately and quickly.

Systems and methods for providing a framework for supporting a self organizing / optimization network (SON) feature in an over the air communications system. An enhanced mobile communications device is provided that performs functions to creating a framework for enhancing SON algorithms. The communications device stores event analysis data in an on board memory and, on certain conditions, signals the analysis to the network. The analysis includes information useful in performing SON algorithms at the network level, such as cell coverage and neighboring cell signal level information, cell selection and reselection information at different locations, relative signal strength between cell base stations at different locations, and coverage hole information. Utilizing the stored information, the network can automatically perform SON algorithms to improve network efficiency without the need for manual intervention by an operator. Embodiments include event filtering by the communications device to efficiently utilize the on board storage.

The embodiment of the invention discloses a network coverage detection method, which comprises the following steps of: receiving a measurement report, which is reported by a terminal which meets preset reporting conditions, by a network side, wherein the preset conditions are associated with cell signal strength; and determining the network coverage condition among neighboring cells according to the measurement report. When the network coverage detection method of the embodiment of the invention is used, a road test step can be saved and the network optimization cost is reduced. The embodiment of the invention further comprises a network coverage detection system and a base station and a terminal used for the network coverage detection system.

This invention is directed to a method for preparation of a biological sample for measurement of protein epitopes that allows for the preservation of intracellular protein epitopes and detection of signal transduction pathways based on the ability to capture transient activation states of the epitopes. The method provided by the invention allows for the rapid fixation of biological samples containing red blood cells, to ensure that epitopes of signal transduction molecules and other intracellular protein epitopes are preserved in the active state. The method of the invention further allows for lysis of red blood cells, thereby making it a useful method for cytometric analysis of biological samples, including, for example, whole blood, bone marrow aspirates, peritoneal fluids, and other red blood cell containing samples. The invention also provides a method to recover or “unmask” epitopes on intracellular antigens that have been made inaccessible by the cross linking fixative necessary to fix the sample. Significantly, the methods of the invention allow preservation and analysis of phospho-epitope levels in biological samples taken directly from patients to determine disease-specific characteristics.

Non-volatile memory (NVM) cells are sensed using a forced neighbor signal to eliminate improper readings generated by a neighbor effect. A selected NVM cell is sensed using a near-ground signal by applying a potential to a first terminal, coupling a second terminal to ground, and then decoupling the second terminal and comparing the resulting cell signal with a reference signal as both signals are developing (i.e., increasing from ground). A forced neighbor signal is applied to one more neighboring cells such that as the sensed cell signal develops (increases from ground), the forced neighbor signal develops at a similar rate, thereby maintaining a voltage across the neighboring cells close to zero and thus preventing leakage of the sensed cell signal through the neighbor cell(s). A dc sensing approach utilizes a current source and grounded resistor to minimize leakage through the neighbor cell(s).

The present invention relates to novel polypeptides that bind to IL-23 receptor and inhibit the binding of IL-23 to its corresponding receptor and cell signaling thereof. The novel polypeptides of the present invention has a core structure of WX1X2X3W, where W is tryptophan, and X1, X2 and X3 are amino acids, with the proviso that when one of X1, X2 or X3 is W, the remaining two of X1, X2 or X3 cannot be W. The present invention relates a composition containing the novel polypeptides, and use of same in treating IL-23 associated human diseases including, for example, inflammatory bowel diseases, psoriasis and Crohn's disease.

Disclosed herein is a method which allows the cell phone operator to download traffic information on the operators cell phone display with a minimum of keystrokes. The cell phone can have a option mode, voice activation or a sequence of keystrokes programmed within the cell or mobile phone to facilitate access of the cell or mobile phone to regional traffic information. This is accomplished by the cell or mobile phone service provider procuring traffic information and data from traffic information provided by an entity such as Traffic Pulse. Alternately the cell phone service provider can make internet access to a traffic information provider directly to the operator of the cell phone. Besides having to first access the internet from a cell phone, and then second navigate to a traffic information website, an operator must provide location information, such as current position through zip code, address, or longitudinal and latitudinal coordination. Cell and mobile phone technology already has the function to monitor the locations of a cell or mobile phone to access various cell phone transmitting and receiving towers in close proximity to the cell or mobile phone. This is accomplished by one example known as triangulation measurement or hyperbolic multi-lateration positioning of cell signals between cell towers or through GPS technology incorporated in the cell or mobile phone. Therefore, the use of current location information of a cell phone acquiring regional traffic data does not need to this information be input, using keystrokes on the cell phone keyboard, or by voice annunciations by the cell phone operator.

A gas sensor system includes an ammonia-sensing cell for generating a signal upon exposure to an unknown gas comprising ammonia, an A / F cell for generating a signal upon exposure to hydrocarbons in the gas, a heater in thermal communication with the cells and a housing in which the cells and the heater are mounted. The housing permits an unknown gas to flow therethrough for contact with the cells, and there is a sensor control circuit in communication with the cells. The sensor control circuit is configured to utilize the signals from the cells to generate an ammonia concentration signal indicating the concentration of ammonia in the unknown gas. Ammonia may be sensed in an unknown gas by heating such cells to selected working temperatures, exposing them to an unknown gas, obtaining signals from the cells, and using the cell signals to determine the ammonia content.

The invention provides a chimeric antigen receptor (CAR) (a) an antigen binding domain of HN1 or SS, a transmembrane domain, and an intracellular T cell signaling domain, or (b) an antigen binding domain of SS1, a transmembrane domain, an intracellular T cell signaling domain, and a granulocyte-macrophage colony-stimulating factor (GM-CSF) receptor 2 leader. Nucleic acids, recombinant expression vectors, host cells, populations of cells, antibodies, or antigen binding portions thereof, and pharmaceutical compositions relating to the CARs are disclosed. Methods of detecting the presence of cancer in a mammal and methods of treating or preventing cancer in a mammal are also disclosed.

The present invention relates to novel polypeptides that bind to IL-23 receptor and inhibit the binding of IL-23 to its corresponding receptor and cell signaling thereof. The novel polypeptides of the present invention has a core structure of WX1X2X3W,where W is tryptophan, and X1, X2 and X3 are amino acids, with the proviso that when one of X1, X2 or X3 is W, the remaining two of X1, X2 or X3 cannot be W. Preferred core structures include WVDYW or WQDYW. The present invention relates a composition containing the novel polypeptides, and use of same in treating IL-23 associated human diseases including, for example, inflammatory bowel diseases, psoriasis and Crohn's disease.

The invention discloses a gene control device and method based on CRISPR-Cas9. The device comprises a nucleotide sequence, wherein the nucleotide sequence is an sgRNA itself or can be transcribed into an sgRNA, the sgRNA structurally comprises a first part bonded with inactivated Cas9 protein and a second part connected to the 3'end of the first part, the 5'end of the first part is provided with an antisense sequence, and the second part is an aptamer sequence part which comprises a ligand binding part and an aptamer stem part; when no ligand exists, the aptamer sequence is pair-bonded with the aptamer stem part; when a ligand exists, the aptamer sequence is dissociated from the aptamer stem part so as to be pair-bonded with a target DNA. By integrating an RNA riboswitch bonded with a specific biological signal into the sgRNA, a bridge of cell signal intensity and gene expression intensity is established, any target gene can be activated or silenced on the basis that biological signal identification is achieved, and artificial connection is built between signal ligands and cytogene.

The present invention provides for chimeric Wnt antagonists comprising a Frz domain component derived from a Frizzled protein, a secreted Frizzled related protein or Ror protein and an Fc immunoglobulin component, and their use in the treatment and diagnostic detection of cellular Wnt signaling and Wnt-mediated disorders, including cancer.

The invention belongs to the technical field of molecular biology and relates to application of an exosome derived from human mesenchymal stem cells to preparation of medicines for antagonizing scars or tissue and organ fibrosis. According to the application, the high-purity and high-bioactivity exosome is obtained by subjecting healthy human umbilical cords to primary in-vitro culture, collecting liquid supernatant after amplification, performing low-temperature ultracentrifugation, purifying and the like; the exosome is used individually or added into medicines, health care products and cosmetics according to a proportion to be developed into a product with a bioactivity function, thereby being capable of regulating organism cell signal transduction effectively, inhibiting fibrocyte differentiation and then antagonizing the tissue and organ fibrosis and scar forming. Through experimental verification, the exosome can play a remarkable role in repairing promotion in hypertrophic scar after skin injury, particularly pathological fibrosis proliferation such as scar contracture of the joint parts, hepatic fibrosis and cardiac fibrosis, and is high in safety, little in toxicity and convenient to storage and transport, thereby being wide in application prospect.

The present invention provides high speed mobile terminal cell switchover control method. Said method includes when terminal moves away current service base station at higher set traveling speed and approaches predicted target cell, network side judges target cell signal whether satisfying pre-switching condition, if satisfying then starting up pre-switching and establishing channel in target cell. Said invention realizes high speed mobile terminal switching through predetermining mobile terminal travel direction, pre-applying channel resource in target cell and establishing channel.

The invention provides a determination method for a voice service scheme of a VoLTE (Voice over Long Term Evolution) terminal and the VoLTE terminal. The method comprises the following steps: reselecting from a 2G / 3G (Two-Generation / Three-Generation) cell to an LTE cell by the VoLTE terminal according to cell signal intensity measured in a preset range; if a reselected LTE cell identity is stored at the VoLTE terminal, transmitting a first position updating request to a mobile management entity MME by the VoLTE terminal, wherein a VoLTE voice service scheme restriction identity is carried in the first position updating request, so an IMS (IP Multimedia Subsystem) unregistration request message of the VoLTE terminal is transmitted to an IMS by a home subscriber server HSS according to the VoLTE voice service scheme restriction identity carried in a first position updating message; receiving, by the VoLTE terminal, an IMS unregistration success response message transmitted by the IMS; determining circuit switched fallback CSFB according to the IMS unregistration success response message to serve as the voice service scheme of the VoLTE terminal to perform the voice service.

The invention relates to a culture medium for preparing neural stem cells and an application of the culture medium. The culture medium for preparing the neural stem cells comprises a culture medium suitable for the growth of stem cells, and a cell signal pathway inhibitor which is selected from at least one of the following agents: a GSK (Glaxo Smith Klein) inhibitor, an MEK (Methyl Ethyl Ketone) inhibitor, a TGF (Transforming Growth Factor)-beta inhibitor, a ROCK inhibitor and a BMP inhibitor. The somatic cells are cultured by using the culture medium, especially the somatic cells for expressing the transcription regulator are cultured, so as to effectively transdifferentiate the somatic cells into neutral stem cells and the transdifferentiation time is greatly shortened.

Signal Transducer and Activator of Transcription (STAT) proteins have a fundamental role cell signaling, and are activated by a large number of cytokines and growth factors. One member of the STAT family, STAT3, has a critical role in oncogenesis. The present invention relates generally to disruption of the pathway of STAT3 signaling in the treatment of human cancer. STAT3 activation is shown to be present in diverse tumor cell lines and tumors, to promote oncogenesis, to inhibit apoptosis, and to reduce sensitivity to chemotherapeutic agents. Inhibition of STAT3 signaling induces apoptosis specifically in tumor cell lines, and increases sensitivity to chemotherapeutic agents. The invention relates more particularly to methods, compositions, means of administering such compositions, and means for identifying such compositions for the inhibition of STAT3 intracellular signaling in the treatment of human cancers.