22 results about "Mature cell" patented technology

The invention discloses a culture method for improving proliferation and differentiation capacity of NK (Natural Killer) cells based on angelica sinensis-astragalus membranaceus exosomes, which comprises the following steps: firstly extracting exosomes in angelica sinensis and astragalus membranaceus, then adding the exosomes into an NK cell culture system according to a specific concentration, and regulating and controlling an NK cell signal channel by virtue of active ingredients carried by the exosomes so as to improve the proliferation and differentiation capacity of the NK cells. Therefore, the proliferation rate and differentiation maturity of the NK cells are remarkably improved. Experimental results show that compared with a traditional culture method, the proliferation multiple of the NK cells cultured through the method is increased by 28.99%-33.69%, the proportion of differentiated mature cells is increased by 15.91%-28.30%, and the cell killing activity is not remarkably reduced. The method can be widely applied to the field of immune cell treatment, provides a high-quality NK cell source for clinic, effectively solves the problems of slow cell proliferation rate, low differentiation maturity, easy activity reduction after large-scale culture and the like in traditional NK cell in-vitro culture, and has important clinical application value and industrialization prospect.

Disclosed herein include methods, compositions, and kits suitable for use in cell therapy. In some embodiments, there are provided methods and compositions for differentiating stem cells into pancreatic beta cells capable of producing insulin.

A method of generating cells of the T cell lineage is provided that involves culturing a sample comprising stem cells or progenitor cells with an engineered Notch ligand conjugated to a suspension support and isolating cells of the T cell lineage. In one embodiment, the cells of the T-cell lineage are progenitor T cells or mature T cells. Compositions, kits and uses thereof are also provided.

The application belongs to the technical field of biological medicine and nanomaterials, and particularly relates to a kind of bionic nano drug delivery system, preparation method and application in eye immunotherapy.The application is with mesoporous SiO2 Nanomicrosphere as core, after protonation treatment, load sCD83, and coat mature DC cell membrane, form DCM@MSN / sCD83 nano drug delivery system.The preparation method of the application has high biocompatibility and simple operation, and the system can actively target mature DC after vitreous injection, release sCD83 and induce mature DC to transform into resistant DC, secrete IL-10 and TGF-β and other anti-inflammatory factors, inhibit STING path activation, reduce retinal inflammatory damage;At the same time, it can penetrate blood-ocular barrier into peripheral circulation, induce mature DC differentiation in neck lymph nodes and spleen and other immune organs, realize local and systemic synergistic immunoregulation, and can be used for preparing medicaments for treating experimental autoimmune uveitis.

The current disclosure provides methods and compositions that provide for the insertion of transgenes in stem or progenitor cells without the deleterious effects to T cell differentiation by in vitro T cell differentiation methods. To accomplish this, the inventors have discovered that expression of the transgenes under the control of promoter regions, such as CD8a or CD8b, allows for the coordinated expression pattern that provides for both: 1) high expression of the transgene in mature T cells and 2) a coordinated level of expression of the transgene throughout the in vitro differentiation method that allows for the production of a population of mature T cells.

There is provided a method of generating T cell lineage cells, the method comprising (a) culturing a sample comprising stem cells or progenitor cells with a soluble multispecific ligand comprising a Notch ligand and a functional protein partner (FPP) that binds to a surface molecule on the stem cells or progenitor cells, and (b) isolating the T cell lineage cells. In one embodiment, the T cell lineage cells are progenitor T cells or mature T cells. Multispecific ligands, compositions, kits and uses thereof are also provided.

The invention discloses a pancreatic beta cell precursor cell transplantation chip based on human-derived iPSC (induced pluripotent stem cell) as well as a preparation method and application of the pancreatic beta cell precursor cell transplantation chip. The chip is composed of beta cell precursor cells (expressing Pdx1, Nkx6.1 and NeuroD1) and a porous degradable bracket (PCL / hyaluronic acid composite material), wherein the beta cell precursor cells (expressing Pdx1, Nkx6.1 and NeuroD1) are obtained by performing four-stage induction on autologous iPSC. The preparation method comprises the steps of autologous iPSC acquisition, beta cell precursor cell induction, three-dimensional bracket preparation and chip assembly. After the chip is implanted into the pancreas of a patient through minimally invasive surgery, precursor cells are differentiated into mature beta cells in vivo, insulin is secreted, the stent is gradually degraded, the pancreas islet function can be fundamentally improved, and the chip is used for treating type I and type II diabetes mellitus. The invention avoids immunological rejection, improves cell survival and functional stability, and has significant clinical transformation value.

In an aspect, a method of making a humanized animal includes providing an immune-deficient animal lacking mature T cells, B cells and NK cells; and injecting a population of human thymocytes into the immune-deficient animal to provide the humanized animal; wherein the population of human thymocytes are thymocytes collected from human thymus or thymus tissue; and wherein the humanized animal includes mature human T cells expressing human CD8 and human CD4. In another aspect, also included is a humanized animal prepared by the foregoing method.

A gene delivery system and applications thereof in the technical field of biological medicines that is particularly useful in the preparation of drugs for treatment of tumors are disclosed. The gene delivery system and applications relate to technology for inducing the differentiation of malignant tumor cells into mature cells, in which regulating the expression of HNF4 alpha protein in the malignant tumor cells using messenger ribonucleic acid, the malignant phenotype of the malignant solid tumor cells is inhibited, and the effective treatment of the malignant solid tumors is achieved. The gene delivery system and applications are therefore applicable to a method of preparing a drug for treating malignant solid tumors and to a method of treating a patient having a malignant solid tumor.

The invention discloses an intelligent artificial bionic DC cell nanoparticle nDC-M and a preparation method, application and a detection method thereof. The nano-particle is of a core-shell structure, the inner core is a hypoxia response type nano-micelle of a sound-sensitive agent SP8 and IL-2 for activating T cells to be mature, the outer layer is coated with a mature DC cell membrane, and the nano-particle has the immune recognition characteristic and the treatment function. In a tumor hypoxic microenvironment, the micelle specifically releases SP8 and IL-2; sP8 generates ROS through ultrasonic excitation, tumor cell immunogenicity death is induced, and antigens are released; iL-2 activates proliferation and functions of tumor infiltration lymphocytes; the outer DC cell membrane and the antigen capture functional group enhance the collection and activation of immune cells through surface antigen presentation, and amplify the anti-tumor immune response. By combining sonodynamic therapy and immunoregulation, tumor growth is inhibited, metastatic load is reduced, good biocompatibility and safety are achieved, and a novel bionic delivery system is provided for metastatic tumor immune combined therapy.

A gene delivery system and applications thereof in the technical field of biological medicines that is particularly useful in the preparation of drugs for treatment of tumors are disclosed. The gene delivery system and applications relate to technology for inducing the differentiation of malignant tumor cells into mature cells, in which regulating the expression of HNF4 alpha protein in the malignant tumor cells using messenger ribonucleic acid, the malignant phenotype of the malignant solid tumor cells is inhibited, and the effective treatment of the malignant solid tumors is achieved. The gene delivery system and applications are therefore applicable to a method of preparing a drug for treating malignant solid tumors and to a method of treating a patient having a malignant solid tumor.

The invention discloses a method for obtaining, activating and amplifying natural killer cells through iPSC differentiation induction, and relates to the technical field of stem cell biology, the method for obtaining, activating and amplifying natural killer cells through iPSC differentiation induction comprises the following steps: S1, preparing and inoculating iPSC cells; s2, differentiating the iPSC to a mesoderm; s3, the mesoderm cells are differentiated to CD34 + hematopoietic endothelial cells; s4, differentiating the CD43 < + > / CD45 < + > hematopoietic stem cells into the iNK cells; s5, maturation of the iNK cells; s6, activating and amplifying the iNK cells; in the step S1, cells are resuspended by using a fresh E8 complete culture medium and are inoculated into a porous culture plate; a plurality of induction culture media are used in the culture process; by adopting a porous culture plate and using an E8 complete culture medium to resuspend cells, efficient inoculation and primary culture of iPSC are realized, and the survival rate and amplification efficiency of cells are improved; the differentiation process of the iPSC towards the mesoderm, the hematopoietic endothelial cells and the CD34 + hematopoietic stem cells is optimized by using various induction culture media, and the differentiation efficiency is improved.

The current invention provides for methods and systems of inducing cell cycle exit and terminal differentiation in stem cells undergoing differentiation into various mature cell types in particular pancreatic endocrine cells. The current invention also provides for methods and systems of inducing differentiation of pancreatic endocrine cells from stem cells. The invention also provides for the cells produced by the methods that are suitable for transplantation or grafting into a subject for the prevention and / or treatment of disease, and useful for basic research and drug testing.

To provide methods, compositions, and kits for generating progenitor and mature T cells, and uses of the cells.SOLUTION: A method of generating cells of the T cell lineage is provided comprising: (a) culturing a sample comprising stem and progenitor cells with a Notch ligand conjugated to a suspension support; and (b) isolating cells of the T cell lineage. In one aspect, the cell of the T cell lineage is a primary T cell or a mature T cell. Compositions, kits, and uses of the cells are also provided.SELECTED DRAWING: Figure 1

A method of generating cells of the T cell lineage is provided comprising (a) culturing a sample comprising stem cells or progenitor cells with a Notch ligand conjugated to a suspension support and (b) isolating cells of the T cell lineage. In one embodiment, the cells of the T-cell lineage are progenitor T cells or mature T cells. Compositions, kits and uses thereof are also provided.

The application discloses a method for simulating wood formation and radial growth of broad-leaved trees by using climate data and belongs to the technical field of tree growth prediction. In order to solve the problem of accurate simulation of wood formation and radial growth of broad-leaved trees, the application comprises the following steps: collecting input data including latitude data of a target region and long-term daily meteorological data of the target region; establishing cambium activation conditions of the broad-leaved trees; calculating relative growth rates of cell expansion, cell wall deposition and cambium cells, and calculating expansion rates of the cambium cells; establishing a cambium cell division model and a vessel cell differentiation cycle model; establishing a cell expansion model and a cell wall thickening model, simulating the growth process of the cells in the xylem until the cell wall lignification process is completed, and then the cells enter a mature cell stage; and based on the mature cell stage, calculating annual ring width and anatomical characteristics, and completing the simulation process of simulating the wood formation and radial growth of the broad-leaved trees by using the climate data.

The application discloses application of MST1 interfering RNA in promoting in-vitro differentiation of ESCs into insulin-secreting cells, and belongs to the technical field of biotechnology. In the process of in-vitro directional differentiation of rat ESCs into beta cells, the application infects by using an MST1 shRNA lentivirus vector, and indexes are detected and functions are determined by using cell biology and molecular biology methods, and it is found for the first time that the efficiency of inducing ESCs to differentiate into beta cell-like cells can be improved by reducing the expression of MST1. Meanwhile, how MST1 realizes the regulation of beta cell maturation by means of PDX1 in the process of directional differentiation of rat ESCs into beta cells is described, the reason affecting in-vitro maturation of beta cells is discussed, and thus the system of directional differentiation of ESCs into mature beta cells is optimized, which has important significance for obtaining beta cells with normal insulin secretion function in-vitro.

New strategies for treating patients suffering from Parkinson's disease and other secondary Parkinson's conditions are disclosed. Disclosed are DA neuronal cells that have been modified in vitro with genetic insertion of GDNF. A GDNF coding sequence is inserted under transcriptional control of a promoter such that after the engineered cell that is to be administered has been mature to a neuronal mature cell type, secretory proteins are produced and uptake by endogenous cells to promote survival of the endogenous neurons. Also disclosed are DA neuronal cells that have been modified in vitro with genetic insertion of GBA and that are SNCA hemizygote-invalid. The GBA coding sequence is inserted under transcriptional control of a traversal promoter and produces secretory proteins after transplantation and uptake immediately by the implant and endogenous cells to promote long term implant integrity.

Reprogramming allows the conversion of any mature or somatic cell of the human or animal body into a pluripotent stem cell. Reprogramming can be performed by introducing exogenous factors, usually transcription factors, into the mature cell. This process allows the production of induced pluripotent stem cells without the use of embryos, with the advantage that they can be produced from an individual for later reimplantation. The inventors have developed a method for the transient expression of exogenous reprogramming factors using a transient vector, in which the vector is a closed linear DNA molecule. Remarkably, the pluripotent stem cells developed in this way are stable and exhibit a phenotype more similar to that of natural stem cells, such as embryonic stem cells (ESCs).

Provided are age-modulated cells and method for making age-modulated cells. The aging and rejuvenation processes can be induced for young, aged, mature and / or immature cells, such as a somatic cell, a stem cell, a stem cell-derived somatic cell, including an induced pluripotent stem cell-derived cell, by contacting cells with one or more age-inducing or rejuvenating agent. Methods described by the present disclosure can produce age-appropriate cells from a somatic cell or a stem cell, such as an old cell, young cell, immature cell, and / or a mature cell. Such age-modified cells constitute model systems for the study of late-onset diseases and / or disorders.

The invention discloses a two-factor fusion protein of Flt3L and IL-7 (interleukin-7) and application of the two-factor fusion protein. Relates to the technical field of biology. According to the fusion protein provided by the invention, two heavy-like chains are connected through a disulfide bond to form a homodimer, and the fusion protein realizes double functions through a single molecule: Flt3L targets Flt3 to drive bone marrow precursor cells to be differentiated into dendritic cells, so that a DC library is expanded; maintaining development, survival and steady state of T cells and B cells through IL-7 is crucial to long-term survival of initial and memory T cells. The two have functional complementarity and are used for respectively promoting differentiation and maturation of DC precursor cells, and the DC cells further activate T lymphocytes; meanwhile, T lymphocytes are supplemented, so that systematic immunoregulation is realized, the fusion protein has disease universality, and a new technical support is provided for immune anti-aging and prevention and treatment of tumor and microbial infection.

The invention provides a mature T cell clonality detection reagent composition and application thereof. The reagent composition comprises five groups of antibodies and can be used for diagnosis and subtype judgment of mature T lymphocyte tumors, during application, four tubes are used in parallel or a first tube is combined with 1-3 tubes in second, third and fourth tubes, and the first group of antibodies, the second group of antibodies, the third group of antibodies and the fourth group of antibodies are respectively used for different tube samples. The reagent composition can be applied to flow cytometry for mature T cell clonality detection and mature T lymphocyte tumor diagnosis and subtype judgment.