16 results about "Mammary cells" patented technology

The invention discloses a nutritional additive for livestock, which is prepared from thiamine and nicotinamide in a mass ratio of (1-2): (1-2), and is a brand-new nutritional additive capable of effectively relieving the industrial problems of sheep breast immunity, oxidative damage, lactation performance reduction and the like caused by heat stress. Under the optimized additive amount, by applying the nutritional additive, the functions of mammary gland cells can be remarkably improved, and the milk yield and the content of milk components such as milk fat, milk protein and lactose can be increased; according to the nutritional additive, a safe and stable daily ration formula can be established, and the residual risk of traditional chemical drugs or excessive vitamins is avoided.

This invention belongs to the field of biomedical technology and discloses the application of olaparib in combination with taquimod in the preparation of anti-tumor metastasis drugs. This invention is the first to discover that olaparib and taquimod, even at low concentrations (i.e., concentrations that do not affect tumor cell proliferation), can effectively inhibit the invasion and metastasis of breast cancer cells, exhibiting high safety. Furthermore, when olaparib and taquimod are used in combination, the inhibitory effect on breast cell invasion and metastasis shows a synergistic enhancement trend, with significantly better efficacy than single-drug therapy, overcoming the limitations of monotherapy. Moreover, the combination of olaparib and taquimod can effectively inhibit lung metastasis of breast cancer, providing a new theoretical basis and medication regimen for anti-metastasis treatment of breast cancer.

The invention discloses a semi-sandwich type metal organic ruthenium complex and a preparation method thereof, and relates to the technical field of medicines, the semi-sandwich type metal organic ruthenium complex is synthesized by researching the structure-activity relationship between an isoquinoline derivative and a pyridine compound, and the structural formula is shown in the specification, r groups comprise hydrogen, C1-C4 alkyl groups, C1-C4 alkoxy groups,-NR12, C6-C16 aryl groups, halogen,-COOR2, cyano groups, nitryl groups, aldehyde groups, acetyl groups, benzoyl groups and phenoxy groups. The invention proves that the semi-sandwich type metal organic ruthenium complex has obvious proliferation inhibition activity on triple negative mammary gland cells and also has higher physiological stability, so that a valuable research direction is provided for developing a new generation of antitumor metal drugs.

Provided herein are cell constructs for producing a cultured milk product from mammary epithelial cells (MECs). In some embodiments, the cell constructs comprise a scaffold, a culture medium in fluidic contact with the scaffold, and mammary cells coupled to the scaffold. In some embodiments, one or more features and / or properties of the scaffold are specified so as to mimic a basement membrane to help to induce the secretory phenotype of mammary epithelial cells in vitro.

The invention provides isolated human breast milk exosomes, and compositions thereof. The present invention also provides in vitro methods for producing said exosomes comprising generating lactocytes derived from human induced pluripotent stem cells (hiPSC), expressing the human milk like product from lactocytes, and purifying the exosomes from the human milk like product. The invention also provides therapeutic uses of the isolated human breast milk exosomes, and compositions thereof.

The present application relates to living cell constructs for the production of cultured dairy products and methods of use thereof. The present invention relates to a living cell construct for the in vitro and / or ex vivo production of a cultured milk product from mammary gland cells, to a method for the production of an isolated cultured milk product from mammary gland cells, to a bioreactor for the production of the isolated cultured milk product, and to a cultured milk product.

Described herein are systems and methods for culturing mammary cells comprising microcarriers and methods of use thereof.

The invention relates to the technical field of biomedicine, and discloses a mammary gland lactation organoid and a preparation method and application thereof. The mammary gland lactation organoid is obtained by induced culture of mammary gland globules; the mammary gland ball is obtained by inducing and culturing a cell aggregate; the cell aggregate is obtained by three-dimensional aggregation culture of MCF10A cells. Through two-stage culture of promoting cell proliferation culture and promoting lactation culture, based on Areg-mediated cell lineage directional differentiation and a PRL-driven hormone activation synergistic mechanism, the mammary gland lactation type organ model with an efficient secretion function is successfully constructed, specific differentiation and lactation function reconstruction of mammary gland cells are realized, and the mammary gland lactation type organ model with an efficient secretion function is obtained. The invention provides a good solution for improving breast milk yield and accessibility, can be used as an important experimental platform for researching breast function regulation and molecular mechanism in the lactation period, and can be widely applied to the related research field of breast lactation.

The invention provides isolated human breast milk exosomes, and compositions thereof. The present invention also provides in vitro methods for producing said exosomes comprising generating lactocytes derived from mammalian mammary epithelial cells, for example human mammary epithelial cells, expressing the mammalian milk like product, for example the human milk like product from lactocytes, and purifying the exosomes from the mammalian milk like product. The invention also provides therapeutic uses of the isolated human breast milk exosomes, and compositions thereof.

The invention discloses a green tea extract, hydrolyzed keratin and vitamin C composition for delaying senescence of cats and dogs, and belongs to the technical field of pet anti-aging food preparation. The composition of the three components can promote the proliferation of cat mammary gland cells and dog mammary gland cells, and obviously improve the cell proliferation capacity decline caused by actinomycin D; meanwhile, the expression level of senescence-related proteins (such as p16, p21, p53 and the like) can be down-regulated, and the up-regulation trend of the senescence-related proteins in a senescence-induced state can be reversed. In addition, the composition has a remarkable protection effect on oxidative damage of cat mammary gland cells and dog mammary gland cells, and is regulated and controlled by a Hippo signal channel. Animal experiment results show that the composition can improve the athletic ability and oxidative stress level of aged mice and delay the aging process of the aged mice. The technical scheme provided by the invention is high in safety and remarkable in biological activity, and has a wide application prospect in the fields of pet anti-aging nutrition intervention and functional medicine development.

The current study was conducted to investigate the possibility Using fenugreek seed extract against cancer cells For humans, two MCF-7 breast lines were also used. For cancer, specifically liver cancer cells And also the Vero HCAM cell line Natural (non-cancerous) as a comparison using Colorimetric. The MTT extract showed an assay Fenugreek seeds exhibit clear activity against alcohol. Staphylococcus and Pseudomonas aeruginosa The alcoholic extract of fenugreek aureus seeds showed Inhibiting and stopping the growth of breast cancer cells without taking Cell death (apoptosis or necrosis) pathway Programmed cell death (or necrosis) occurred in a dose-dependent manner after 72 hours of incubation, while the HCAM extract showed no anticancer effect on cell lines. Fenugreek seed extract showed Vero activity and Antibacterial activity against pathogenic bacteria was tested at various levels. The current MCF-7 study demonstrated the effect of fenugreek seed extract on tissues. HCAM and no effect was observed on the cell line To our knowledge, no study has demonstrated inhibition of human breast cancer cells without the occurrence of apoptosis or necrosis. This finding focuses on the potential benefit of fenugreek extract for the treatment of breast cancer.

The invention relates to the technical field of sow feed processing, and particularly discloses a lactating sow feed capable of increasing the lactation yield of sows and a preparation method thereof.The lactating sow feed is prepared from, by weight, base materials and active materials, the base materials comprise 400-450 parts of corn, 200-230 parts of soybean meal, 38-42 parts of whey powder, 68-72 parts of bran, 19-21 parts of fish meal and 33-37 parts of vitamin and mineral premix, and the active materials are mixed with the base materials; 15 to 18 parts of mineral supplement and 120 to 130 parts of maltodextrin; the active material is prepared from 0.9 to 1.1 parts of enzyme group material, 15 to 25 parts of maltodextrin, 6 to 10 parts of phospholipid oil-loaded microcapsule and 3 to 5 parts of organic zinc-biotin-lysine ternary complex. Sufficient unsaturated fatty acid raw materials are provided for mammary gland cells, so that milk fat synthesis is promoted, and milk quality is improved; the bioavailability of Zn is remarkably improved through a chelating structure, Zn participates in mammary gland metabolism and protein synthesis, lysine promotes milk protein synthesis, and biotin improves fatty acid metabolism and mammary gland epithelium functions and synergistically enhances lactation capacity.

The invention provides isolated human breast milk exosomes, and compositions thereof. The present invention also provides in vitro methods for producing said exosomes comprising generating lactocytes derived from human induced pluripotent stem cells (hiPSC), expressing the human milk like product from lactocytes, and purifying the exosomes from the human milk like product. The invention also provides therapeutic uses of the isolated human breast milk exosomes, and compositions thereof.

The invention discloses application of matrine in preparation of a product for resisting staphylococcus aureus invasion of bovine mammary epithelial cells. The invention provides an application of sophocarpidine in preparation of a product with at least one of the following functions: A1) preventing and treating animal infectious diseases; a2) anti-staphylococcus aureus or methicillin-resistant staphylococcus aureus; a3) reducing the adhesion of staphylococcus aureus or methicillin-resistant staphylococcus aureus to mammary gland cells or tissues; a4) the invasion of staphylococcus aureus or methicillin-resistant staphylococcus aureus to mammary gland cells or tissues is reduced; a5) retarding apoptosis of mammary gland cells or tissues induced by staphylococcus aureus or methicillin-resistant staphylococcus aureus. The matrine can be used for preparing veterinary drugs and preventing and treating animal diseases caused by staphylococcus aureus or staphylococcus aureus drug-resistant strains MRSA.

The invention provides a traditional Chinese medicine composition for enlarging and beautifying breasts and removing stasis and a preparation method of the traditional Chinese medicine composition. The composition is prepared from the following raw materials in parts by weight: 15 to 30 parts of camellia-seed oil, 12 to 28 parts of crithmum maritimum extract, 10 to 25 parts of red clover root extract, 3 to 10 parts of rosemary leaf oil, 1 to 3 parts of jasmine essential oil, 8 to 20 parts of leuconostoc / radish root fermentation product filtrate, 6 to 18 parts of African chandelia fruit extract, 5 to 15 parts of arnica flower extract and 0.1 to 1 part of acetyl hexapeptide-7. And blood-activating and stasis-removing auxiliary raw materials and skin-care components can also be added. During preparation, oil phase-water phase separation, gradient emulsification and vacuum ripening processes are adopted; through the synergistic effect of various active components, the composition can effectively promote mammary gland cell proliferation and adipose differentiation, has the effects of resisting inflammation and improving microcirculation, has the double effects of enlarging breasts and removing stasis and blood stasis, is good in stability, and can be used for preparing cosmetics or external medicines.