56 results about "Mammary cells" patented technology

The present invention features a novel use of processed ingredients from the Indian mulberry plant. More specifically, the present invention features a novel use of processed Morinda citrifolia, namely Morinda citrifolia fruit juice, puree, or puree juice for treating breast cancer, and particularly for inhibiting and/or preventing the metastasis of carcinogenic cells within the mammary region of the breast, as well as destroying metastasized mammary or breast cancer cells. The present invention comprises the consumption of food products or medicinal products or compositions comprising processed Morinda citrifolia, in either puree or fruit juice form. The present invention also features a method of inhibiting carcinogen-mediated conversion of mammary cells and protecting DNA against carcinogen-mediated damage by administering a composition comprising water soluble Morinda citrifolia.

The invention discloses a Chinese herbal medicine feed additive for improving milk yield of dairy cattle and improving dairy quality. The additive contains fermented Chinese herbal medicine compounds with the effects of clearing away heat and toxic materials, invigorating the circulation of blood and promoting lactation, dilating blood vessels, tonifying kidney, qi and spleen and enhancing the body resistance, and sodium selenite and other substances capable of promoting milk yield of dairy cattle are added, and the absorption effect of the dairy cattle on additives and active ingredients can be improved due to existence of probiotics. The Chinese herbal medicine raw materials disclosed by the invention mainly comprise angelica sinensis, astragalus, acanthopanax, Beautiful Sweetgum Fruit, Codonopsis pilosula, the seed of cowherb, sea-buckthorn, dried tangerine or orange peel, motherwort, dandelion, Ligusticum wallichii, liquorice, fevervine and white paeony root. According to the Chinese herbal medicine feed additive, the mammary gland functions can be effectively improved, mammary gland cells are activated, the lactation yield of dairy cattle is improved, the milk production peak is maintained, the milk production quality is improved, and the product is environmentally and safe and does not have the problems of drug residues and drug tolerance.

A method for isolating cells which have a suprabasal position and expresses epithelial specific antigen but no sialomucin is provided. The isolated cells are shown to share many of the properties expected of a mammary gland stem cell. Three permanent cell lines that are capable of proliferating and capable of differentiating into cells of mammary gland luminal epithelial and myoepithelial cell lineages were established. Such cells form elaborate branching structures resembling uncultured terminal duct lobular units both by morphology and marker expression both in vitro and in vivo. Evidence is provided that these keratin K19 expressing cells most probably is the cells in which breast cancer arises. Thus they constitute a model not only for the developing breast, but also for the development of breast cancer. Also disclosed are the uses of such isolated cells or the cell lines as a model system of the mammary gland for pharmacological studies and their uses in tissue repair or transplantation.

Disclosed are methods for diagnosing breast cancer in a cell sample by detecting an increase in the levels of expression of protein markers in the cell sample as compared to the levels of expression of the same protein markers in a normal, nonneoplastic breast cell sample. Also disclosed is a device for diagnosis of cancer in a cell sample.

The invention relates to a separation and culture method for different cellular components of a human mammary tissue, and belongs to the technical field of cell culture. The method comprises the following steps: (1) sampling a fresh human mammary tissue specimen; (2) carrying out mechanical shearing and collagenase and hyaluronidase combined digestion to prepare a mammary tissue cell suspension; (3) carrying out low-speed and differential centrifugation to layer and separate the different cellular components; (4) carrying out centrifugation, washing and adherent culture for multiple times to purify the cell components, so as to obtain the different cellular components of the mammary tissue, which comprise epithelial cells, matrix cells and preadipocytes. The separation and culture method disclosed by the invention can be used for quickly, simply and effectively separating and purifying the different cellular components from the same mammary tissue; the cultured mammary gland cells are sufficient in quantity, good in cell viability and purity which is up to 95% above; the separation and culture method can provide very useful materials for molecular biology study associated with mammary glands and breast cancer, and establishes a foundation for construction of a mammary gland microenvironment multi-cell culture model.

Disclosed herein are methods for detecting and identifying potential breast cancer biomarkers in an individual patient. Also disclosed are newly discovered breast cancer markers set forth in Tables I, II and III, associated with the cancerous state of breast cells. It has been discovered that a higher than normal level of expression of any of these markers or combination of these markers correlates with breast cancer in a patient. Methods are provided for detecting the presence of breast cancer in a sample, the absence of breast cancer in a sample, the stage of breast cancer, assessing whether a breast cancer has metastasized, predicting the likely clinical outcome of a breast cancer patient, and with other characteristics of breast cancer that are relevant to prevention, diagnosis, characterization, and therapy of breast cancer in a patient.

A cell culture article includes a porous substrate having a plurality of pores and a plurality of interstices in communication with the pores. At least some of the plurality of pores and interstices are sufficiently large for two or more mammary epithelial cells to cluster within the pores or interstices. Non-malignant mammary epithelial cells or breast cancer cells may not attach strongly to the substrate surface, which may encourage cell-cell interaction. In many cases, the article is desirably free of components of unknown origin. The articles may be capable of maintaining culture of malignant and non-malignant mammary epithelial cells and allowing for development of in vivo-like morphologies or characteristics of such cells.

The invention discloses application of a set of miRNAs in preparation of a marker for screening early-stage breast cancers. The invention discovers that free miRNAs with completeness and relative abundance quite accordant with mammary gland cells exist in human breast milk for the first time. Through comparison with abnormal miRNAs which are abnormally expressed by the breast cancers and are confirmed by existing researches, a set of breast milk free miRNAs suitable for potential application of the screening of the early-stage breast cancers are analyzed, 26 miRNAs are expected to be regulatedup in the development process of the early-stage breast cancers, and 12 miRNAs are expected to be regulated down in the development process of the early-stage breast cancers. The invention disclosesa theoretically feasible application detection scheme. By taking breast milk as a sample, the affection risk of the early-stage breast cancers is detected by detecting free miRNA markers related withthe breast cancers in the breast milk.

Improved colony forming cell (CFC) assays are described. The improved assay comprises modifications to the standard CFC assay that enable analysis of temporal, real-time changes in antigen expression during colony development without need to fixate or destroy the culture. The improved assay is applicable to hematopoietic CFC assays as well as to CFC assays for other cells, such as neural cells and mammary cells. In one embodiment, the invention comprises adding a detection reagent, most likely a fluorescently labeled antibody, that is specific for antigens (most likely a cell-surface antigen) expressed on progenitors or on specific mature cell types to a culture at the start or during culture. The invention also comprises modifications to the culture medium and cell preparations used in standard CFC assays to selectively promote the development of one colony type while preventing or suppressing the development of other colony types.

The invention discloses a composition capable of enlarging and beautifying breasts, cream and preparation and use methods of cream. The composition is prepared from Lepidium meyenii, dandelions, panax notoginseng, saffron crocus, Anoectochilus roxburghii, green tangerine peels, luffa vegetable sponge, radix rhapontici, swallowwort roots, pearl essence and artemisia argyi powder. The cream is further prepared from the composition. The invention further discloses preparation and use methods of the cream. The composition and the cream, disclosed by the invention, are completely free of hormones and have obvious effects of repairing mastatrophy, external expansion, drooping, flatness and wrinkling and beautifying, enlarging and nursing flat breasts caused by maldevelopment; the composition and the cream can be used for promoting secondary development of the breasts, activating mammary glandular cells through pyrogenic dressing, massage and negative pressure, promoting the blood circulation and rapid fat aggregation of the breasts, prompting mammary growth and enabling the breasts to be full, mellow, dense and compact. In the opinion of a pyrogenic-dressing stasis removing theory of traditional Chinese medicine, pure herb essences are used for dredging channels and collaterals of the breasts and activating blood circulation to dissipate blood stasis, and thus, the breasts can be healthily nursed.

Loss of Wnt-5a protein expression in breast carcinoma patients is associated with a shorter recurrence-free survival as well as increased motility in mammary cell lines. Based on sequence analysis of Wnt-5a 13 peptide fragments were identified and investigated for their ability to mimic effects of the Wnt-5a protein on mammary cell adhesion and motility. Two of these peptides significantly increased adhesion and impaired the motility of non-tumourigenic breast cancer cell lines, both low in endogenous Wnt-5a protein expression. To identify the shortest possible peptide that still had an anti-motile effect, sequential deletions of two Wnt-5a protein expression. To identify the shortest possible peptide that still had an anti-motile effect, sequential deletions of two amino acids from the N-terminal side of the shorter of these two peptides were performed. The effect on tumour cell adhesion was gradually lost, and when only 6 amino acids remained the effect was not detectable. However, formulation of the N—terminal methionine of this hexapeptide restored its effect on adhesion and reduced tumour cell motility. The novel formyl-Met-Asp-Gly-Cys-Glu-Leu peptide ligand can serve as a lead substance for anti-metastatic treatment in the 50% of human breast cancers where the endogenous expression of Wnt-5a is reduced.

Mammastatin has an approximate molecular weight of 44 kDa in its inactive, non-phosphorylated form. Normal mammary cells express functional phosphorylated forms having approximate molecular weights of 53 kDa and 49 kDa. Metastatic mammary cells either do not express Mammastatin at all, or do not express the 53 kDa or 49 kDa, phosphorylated forms. Mammary cancer cells are inhibited in their growth by the administration of phosphorylated Mammastatin.

The invention discloses a culture method of a Jinhua pig mammary gland epithelial cell line. Jinhua pig lactation-period 21-day mammary tissues are subjected to primary culture and subculture to finally obtain the high-purity high-viability Jinhua pig mammary gland epithelial cell line. The mammary gland cell line belongs to the field of biology. The cultured mammary gland cell line is free of fibroblasts and other impure cells, and has high cell purity. The cryopreserved cells have stable quality. The viability of the cryopreserved and resuscitated cells can reach 90%. The cells grow stably after being subjected to subculture, and are suitable for large-scale culture. The cytological characteristic detection indicates that the cell line satisfies the cell line establishment requirement. The Jinhua pig mammary gland epithelial cells can be used for preparing a nucleus transplantation donor of a transgenic pig. The Jinhua pig mammary gland epithelial cell line can provide a raw material for research in medicine and molecular biology. The Jinhua pig mammary gland epithelial cell line can enrich and improve the local pig species in agriculture, and is an effective protection means for favorable pig species in China.

The present invention relates to an improved method that permits the differential isolation of mouse mammary stem cells and colony forming cells (CFCs). The method involves depletion of non-epithelial cells from freshly dissociated mouse mammary tissue by incubation with an antibody composition containing antibodies specific for CD45, Ter119, CD35 and optionally CD140a. After formation of conjugates between the non-epithelial mammary cells and the antibodies specific for CD45, Ter119, CD35 and optionally CD140a, the cell conjugates are removed and the remaining epithelial cells are then incubated with an antibody composition containing antibodies specific for CD24 and CD49f. After formation of conjugates between the epithelial cells and the antibodies specific for CD24 and CD49f, the mouse mammary stem and the luminal-restricted CFC cells can be differentially isolated. The invention also relates to kits for carrying out this method and to the cell preparations prepared by this method.

The invention discloses a method for inducing differentiation of iPS cells to mammary glands in vitro and a special culture medium for the method. The in-vitro induction method comprises the followingsteps: dissociating the iPS cell into single cells, inoculating an iPS cell maintaining culture medium with the single cells as seed cells, and obtaining high-density monolayer cells; culturing the monolayer iPS cells in a differentiation culture medium I, and changing solutions for the cells every day; discarding the culture medium, culturing the cells with a differentiation culture medium II, and changing solutions for the cells every other day; discarding the culture medium, culturing the cells with a differentiation culture medium III, and changing solutions for the cells every other day;and discarding the culture medium, and continuously culturing the cells with a differentiation culture medium IV. The method can obtain a large quantity of single mammary gland cells at different differentiation stages, has the advantages of being simple to operate, low in cost, high in differentiation efficiency and the like, and has broad application prospects.

The present disclosure describes a study of estrogenic activity present in various plant species, selectively inducing some but not all estrogenic responses in the uterus. Prepubertal female rats were treated sequentially with various extracts or decoctions of different plant species or its vehicle, followed 1 h later by treatment with estradiol-17β (E) or its solvent. Uteri were excised under anesthesia and histologically processed for eosinophil quantification and morphometric evaluation of various uterine responses to estrogen, at 6 or 24 h after hormone or vehicle treatment. Besides extracts or decoctions, pure phytoestrogens were also used. Additionally, human mammary cancer cells MCF-7 or MDAMB-231 were cultured in presence of the extract (or decoction), E, both or solvent and cell proliferation was evaluated. Various extracts or decoction displayed selective estrogenic and/or antiestrogenic action for some but not all parameters of estrogen stimulation in the uterus and inhibited growth of human mammary cells in culture or antagonized the estrogen-induced increase in their growth. Present results reveal, for the first time, a dissociation of responses to estrogen by phytoestrogens, suggesting its possible therapeutic application as estrogenic compounds not inducing cell proliferation and reveal the anticancerous effect of some of the extracts with possible therapeutic relevance. The dissociation of responses to estrogen additionally suggest therapeutic applications in estrogen-related diseases (for instance, premenstrual syndrome, endometriosis, etc.); the inhibition of eosinophil degranulation suggest an application in diseases related to eosinophils (hypereosinophilic syndrome, allergic and hypersensitivity diseases).

The invention discloses a ten-medicine Xiang Lu, a preparation method thereof and a ginsenoside content analysis method, and belongs to the technical field of traditional Chinese medicine formulas. The capsule gives full play to the values of the traditional Chinese medicines, has the effects of soothing liver-qi stagnation, softening hardness to dissipate stagnation and the like, the pharmacodynamic material basis of ginseng is ginsenoside which has an adjusting effect on the neuroendocrine function of the pituitary-gonad axis and can excite the pituitary to secrete gonadotropin, promote biosynthesis of DNA and protein synthesis related zymoprotein and increase the gonad activity. Through the two-way regulation effect on the pituitary-gonad axis, the positive and negative feedback functions of neuroendocrine are balanced so as to achieve the purpose of reducing estradiol which is abnormally increased, and mammary gland cell proliferation controlled by ovarian hormone is inhibited; andinternal components of traditional Chinese medicines are selected as internal standards in a quantitative analysis of multiple components by single marker, and the content of the to-be-detected components is calculated by measuring relative correction factors (RCF) between the internal standards and other to-be-detected components, so that the use amount of reference substances is reduced, and the cost is saved.

The present invention relates to a cow milk protein fatty acid modifier capable of inducing human breast cancer cell apoptosis, and a preparation method thereof, and belongs to the technical field of medicine or function food preparation. According to the method, cow milk albumin is subjected to a decalcification treatment to form the unfolded state, and then an unsaturated fatty acid is adopted to carry out appropriate molecule modification on the lactoalbumin component through the anion exchange chromatography technology to form the milk protein fatty acid modifier, wherein the in vitro cell model test results of the modifier prove that the modifier can significantly induce the human breast cancer cell (including estrogen receptor positive cells and estrogen receptor negative cells) apoptosis and does not significantly affect the normal human breast cells. According to the present invention, the support can be provided for development of the breast cancer prevention and treatment drug and the anti-breast cancer function food, the new reference is provided for the high value utilization of the whey protein and the optimization of the formula milk powder, and the positive significance is provided for promotion of the fine and deep processing of the milk product.

The invention belongs to the technical field of sow breeding, and particularly relates to a composite additive bag for promoting mammary gland development of replacement gilts and application of the composite additive bag. The composite additive bag is prepared from an additive and a carrier; the additive is composed of cellulose, inulin, silymarin, baicalin and nicotinic acid; and the carrier iscomposed of zeolite powder and defatted rice bran. The composite additive bag for promoting the mammary gland development of the replacement gilts is researched and prepared according to an apoptosismechanism of mammary gland cells of the replacement gilts, physiological characteristics and reproductive physiological characteristics of mammary gland development of the replacement gilts and physicochemical characteristics of nutrient substances eaten by the replacement gilts. It is found that the weight of mammary glands of the replacement gilts in a third estrus period can be increased, apoptosis of the mammary gland cells in puberty is inhibited, development of mammary gland parenchyma is promoted, then lactation capacity of the gilts in a lactation stage is improved, and productivity and economic benefits of a farm are improved.