27 results about "Mammary cells" patented technology

The invention provides a mammary gland pathological cell image detection method and system based on an improved YOLOv7 model, and the method comprises the steps: S1, collecting mammary gland pathological section images, and generating a data set; s2, carrying out frame labeling on cells obtained in the data set; s3, segmenting the marked data set into a training set and a verification set; step S4, establishing a YOLOv7 network, and respectively adding an ASFF module and a CBAM module in three branches of the YOLOv7 network to obtain an improved YOLOv7 network; and S5, inputting the training set into the improved YOLOv7 network for training, and verifying to obtain the high-precision breast pathological section cell detection model. The improved model can effectively avoid a large semantic gap between non-adjacent levels of mammary gland cell characteristics, greatly enhance the detection capability of multi-scale cell targets such as tiny early cancer cells and large normal mammary gland tissue cells, and improve the recognition precision of early mammary gland lesion cells.

The invention discloses a mammary gland immunohistochemical Ki-67 image segmentation method based on an attention mechanism, and the method comprises the following steps: obtaining a mammary gland immunohistochemical Ki-67 image, and carrying out the segmentation processing of the mammary gland immunohistochemical Ki-67 image, and obtaining mammary gland immunohistochemical Ki-67 image blocks; taking the breast immunohistochemical Ki-67 image block as input, taking the corresponding cell type classification result as output, and training a pre-constructed breast cell segmentation model; a mammary gland cell segmentation model is constructed based on a VGG16 network and a cooperative attention mechanism of a space and a channel; segmenting a mammary gland immunohistochemical Ki-67 image to be predicted into a plurality of image blocks, and inputting the image blocks into the trained mammary gland cell segmentation model for segmentation; and obtaining a distribution density map of classification results of different types of cells. The invention relates to the technical field of image segmentation, and by coordinating the relationship between spatial features and channel features and enhancing the guiding capability of the features, semantic feature differences are effectively relieved, and the precision, flexibility and adaptability of tumor cell segmentation are remarkably improved.

The present invention discloses the application of aspirin combined with abemacili or in combination with other drugs in the treatment of breast cancer, and belongs to the technical field of tumor therapeutic drugs. The present invention firstly finds that, compared with the single application of abemacili, the combined or coordinated use of aspirin and abemacili can significantly inhibit the proliferation of breast cancer MCF7 and MDA-MB-231 cells and promote the apoptosis of breast cancer MCF7 and MDA-MB-231 cells, and the effect of the combined use of aspirin and abemacili is significantly better than the effect of the single application of abemacili or aspirin; compared with the single application of abemacili, the toxicity to normal human breast MCF10A cells is not enhanced, and it is expected to be used for the treatment of breast cancer, and provide a new drug combination for reducing the cost of breast cancer treatment, improving the treatment effect, and alleviating the suffering of patients.

The invention relates to the technical field of cells, and particularly discloses an amino acid culture medium for promoting synthesis of casein of dairy cow mammary cells and a preparation method. In the culture medium, the addition amount of lysine is 0.74 mmol / L, the addition amount of threonine is 0.22 mmol / L, and the addition amount of phenylalanine is 0.28 mmol / L. Tests prove that the culture medium under the condition of the application can obviously improve the relative proliferation rate and casein expression quantity of dairy cow mammary gland cells compared with a culture medium under 4 times of plasma concentration (optimal amino acid supply mode) of dairy cow. The method for promoting casein synthesis comprises the following steps: culturing cow mammary epithelial cells to P3 generation; when the P3 generation cells grow to 75-85% of the adherent area in a 75 cm < 3 > culture flask, transferring the P3 generation cells into a culture medium without lysine, threonine and phenylalanine, and starving for 16 hours; when the cells are hungry, transferring the cells into the culture medium prepared by the invention to be continuously cultured for 24 hours; and after culture is finished, measuring the relative proliferation rate and casein expression quantity of the cells.

The invention discloses a nutritional additive for livestock, which is prepared from thiamine and nicotinamide in a mass ratio of (1-2): (1-2), and is a brand-new nutritional additive capable of effectively relieving the industrial problems of sheep breast immunity, oxidative damage, lactation performance reduction and the like caused by heat stress. Under the optimized additive amount, by applying the nutritional additive, the functions of mammary gland cells can be remarkably improved, and the milk yield and the content of milk components such as milk fat, milk protein and lactose can be increased; according to the nutritional additive, a safe and stable daily ration formula can be established, and the residual risk of traditional chemical drugs or excessive vitamins is avoided.

This invention belongs to the field of biomedical technology and discloses the application of olaparib in combination with taquimod in the preparation of anti-tumor metastasis drugs. This invention is the first to discover that olaparib and taquimod, even at low concentrations (i.e., concentrations that do not affect tumor cell proliferation), can effectively inhibit the invasion and metastasis of breast cancer cells, exhibiting high safety. Furthermore, when olaparib and taquimod are used in combination, the inhibitory effect on breast cell invasion and metastasis shows a synergistic enhancement trend, with significantly better efficacy than single-drug therapy, overcoming the limitations of monotherapy. Moreover, the combination of olaparib and taquimod can effectively inhibit lung metastasis of breast cancer, providing a new theoretical basis and medication regimen for anti-metastasis treatment of breast cancer.

The present invention discloses a casein micelle with phosphorylation characteristics simulating human milk and a preparation method thereof, belonging to the technical field of dairy product processing. The present invention first uses animal milk casein as raw material, adopts selective precipitation technology to separate casein components, mixes each casein component according to the ratio of human milk casein, and realizes the preparation of composite casein; then dephosphorylates the composite casein to different degrees, and according to the phosphorylation degree and distribution of human milk casein, mixes composite caseins with different phosphorylation degrees to realize the preparation of human milk casein with phosphorylation characteristics; finally, according to the assembly process of natural micelles in mammary cells, simulates human milk by assembling the initial ion environment or simulating human milk by the assembly process (continuous) to reconstruct the simulated human milk casein micelles, so that its casein composition, casein phosphorylation characteristics, mineralization degree, structure and digestibility are similar to those of human milk casein micelles.

The invention discloses a semi-sandwich type metal organic ruthenium complex and a preparation method thereof, and relates to the technical field of medicines, the semi-sandwich type metal organic ruthenium complex is synthesized by researching the structure-activity relationship between an isoquinoline derivative and a pyridine compound, and the structural formula is shown in the specification, r groups comprise hydrogen, C1-C4 alkyl groups, C1-C4 alkoxy groups,-NR12, C6-C16 aryl groups, halogen,-COOR2, cyano groups, nitryl groups, aldehyde groups, acetyl groups, benzoyl groups and phenoxy groups. The invention proves that the semi-sandwich type metal organic ruthenium complex has obvious proliferation inhibition activity on triple negative mammary gland cells and also has higher physiological stability, so that a valuable research direction is provided for developing a new generation of antitumor metal drugs.

Provided herein are cell constructs for producing a cultured milk product from mammary epithelial cells (MECs). In some embodiments, the cell constructs comprise a scaffold, a culture medium in fluidic contact with the scaffold, and mammary cells coupled to the scaffold. In some embodiments, one or more features and / or properties of the scaffold are specified so as to mimic a basement membrane to help to induce the secretory phenotype of mammary epithelial cells in vitro.

The invention provides isolated human breast milk exosomes, and compositions thereof. The present invention also provides in vitro methods for producing said exosomes comprising generating lactocytes derived from human induced pluripotent stem cells (hiPSC), expressing the human milk like product from lactocytes, and purifying the exosomes from the human milk like product. The invention also provides therapeutic uses of the isolated human breast milk exosomes, and compositions thereof.

A method for producing mammary gland cells and a method of producing a mammalian milk like product, for example a human milk like product comprising generating lactocytes derived from mammalian induced pluripotent stem cells (miPSC), for example human induced pluripotent stem cells (hiPSC), and expressing the mammalian milk like product, for example the human milk like product from lactocytes.

The present application relates to living cell constructs for the production of cultured dairy products and methods of use thereof. The present invention relates to a living cell construct for the in vitro and / or ex vivo production of a cultured milk product from mammary gland cells, to a method for the production of an isolated cultured milk product from mammary gland cells, to a bioreactor for the production of the isolated cultured milk product, and to a cultured milk product.

Described herein are systems and methods for culturing mammary cells comprising microcarriers and methods of use thereof.

The invention relates to the technical field of biomedicine, and discloses a mammary gland lactation organoid and a preparation method and application thereof. The mammary gland lactation organoid is obtained by induced culture of mammary gland globules; the mammary gland ball is obtained by inducing and culturing a cell aggregate; the cell aggregate is obtained by three-dimensional aggregation culture of MCF10A cells. Through two-stage culture of promoting cell proliferation culture and promoting lactation culture, based on Areg-mediated cell lineage directional differentiation and a PRL-driven hormone activation synergistic mechanism, the mammary gland lactation type organ model with an efficient secretion function is successfully constructed, specific differentiation and lactation function reconstruction of mammary gland cells are realized, and the mammary gland lactation type organ model with an efficient secretion function is obtained. The invention provides a good solution for improving breast milk yield and accessibility, can be used as an important experimental platform for researching breast function regulation and molecular mechanism in the lactation period, and can be widely applied to the related research field of breast lactation.

An apparatus and method for in vitro milk production includes a reactor assembly having an elongate housing enclosing one or more hollow semi-permeable tubes within an extracellular nutrient medium. A monolayer of lactating cells is formed on the inner surface of the tube. A pump connected to the tube produces a pulsating flow of the lactating medium to induce shear stress on the lactating cells. Variable pump rates subject the lactating cells to different shear stress levels to stimulate milk production. The lactation medium is continuously recycled through the tube until a predetermined milk quality is reached, after which it is harvested. The lactating cells may be extracted from milk or mammary tissue and may be genetically modified to produce milk with design characteristics such as low allergenicity or reduced lactose.

The invention provides isolated human breast milk exosomes, and compositions thereof. The present invention also provides in vitro methods for producing said exosomes comprising generating lactocytes derived from mammalian mammary epithelial cells, for example human mammary epithelial cells, expressing the mammalian milk like product, for example the human milk like product from lactocytes, and purifying the exosomes from the mammalian milk like product. The invention also provides therapeutic uses of the isolated human breast milk exosomes, and compositions thereof.

The invention discloses a green tea extract, hydrolyzed keratin and vitamin C composition for delaying senescence of cats and dogs, and belongs to the technical field of pet anti-aging food preparation. The composition of the three components can promote the proliferation of cat mammary gland cells and dog mammary gland cells, and obviously improve the cell proliferation capacity decline caused by actinomycin D; meanwhile, the expression level of senescence-related proteins (such as p16, p21, p53 and the like) can be down-regulated, and the up-regulation trend of the senescence-related proteins in a senescence-induced state can be reversed. In addition, the composition has a remarkable protection effect on oxidative damage of cat mammary gland cells and dog mammary gland cells, and is regulated and controlled by a Hippo signal channel. Animal experiment results show that the composition can improve the athletic ability and oxidative stress level of aged mice and delay the aging process of the aged mice. The technical scheme provided by the invention is high in safety and remarkable in biological activity, and has a wide application prospect in the fields of pet anti-aging nutrition intervention and functional medicine development.

This invention relates to live cell constructs for in vitro and / or ex vivo production of cultured milk products from mammary cells, methods of producing isolated cultured milk products from mammary cells, bioreactors for producing isolated cultured milk products, and cultured milk products.

A method for producing mammary gland cells and a method of producing a mammalian milk like product, for example a human milk like product, comprising generating lactocytes derived from mammalian adult breast milk stem cells (mBSC), for example from human adult breast milk stem cells (hBSC) and expressing the mammalian milk like product, for example the human milk like product from lactocytes.

The current study was conducted to investigate the possibility Using fenugreek seed extract against cancer cells For humans, two MCF-7 breast lines were also used. For cancer, specifically liver cancer cells And also the Vero HCAM cell line Natural (non-cancerous) as a comparison using Colorimetric. The MTT extract showed an assay Fenugreek seeds exhibit clear activity against alcohol. Staphylococcus and Pseudomonas aeruginosa The alcoholic extract of fenugreek aureus seeds showed Inhibiting and stopping the growth of breast cancer cells without taking Cell death (apoptosis or necrosis) pathway Programmed cell death (or necrosis) occurred in a dose-dependent manner after 72 hours of incubation, while the HCAM extract showed no anticancer effect on cell lines. Fenugreek seed extract showed Vero activity and Antibacterial activity against pathogenic bacteria was tested at various levels. The current MCF-7 study demonstrated the effect of fenugreek seed extract on tissues. HCAM and no effect was observed on the cell line To our knowledge, no study has demonstrated inhibition of human breast cancer cells without the occurrence of apoptosis or necrosis. This finding focuses on the potential benefit of fenugreek extract for the treatment of breast cancer.

The present invention relates to living cell constructs for producing cultured milk products from mammary cells in vitro and / or ex vivo, methods for producing isolated cultured milk products from mammary cells, bioreactors for producing isolated cultured milk products, and cultured milk products.

The invention relates to the technical field of sow feed processing, and particularly discloses a lactating sow feed capable of increasing the lactation yield of sows and a preparation method thereof.The lactating sow feed is prepared from, by weight, base materials and active materials, the base materials comprise 400-450 parts of corn, 200-230 parts of soybean meal, 38-42 parts of whey powder, 68-72 parts of bran, 19-21 parts of fish meal and 33-37 parts of vitamin and mineral premix, and the active materials are mixed with the base materials; 15 to 18 parts of mineral supplement and 120 to 130 parts of maltodextrin; the active material is prepared from 0.9 to 1.1 parts of enzyme group material, 15 to 25 parts of maltodextrin, 6 to 10 parts of phospholipid oil-loaded microcapsule and 3 to 5 parts of organic zinc-biotin-lysine ternary complex. Sufficient unsaturated fatty acid raw materials are provided for mammary gland cells, so that milk fat synthesis is promoted, and milk quality is improved; the bioavailability of Zn is remarkably improved through a chelating structure, Zn participates in mammary gland metabolism and protein synthesis, lysine promotes milk protein synthesis, and biotin improves fatty acid metabolism and mammary gland epithelium functions and synergistically enhances lactation capacity.

The invention provides isolated human breast milk exosomes, and compositions thereof. The present invention also provides in vitro methods for producing said exosomes comprising generating lactocytes derived from human induced pluripotent stem cells (hiPSC), expressing the human milk like product from lactocytes, and purifying the exosomes from the human milk like product. The invention also provides therapeutic uses of the isolated human breast milk exosomes, and compositions thereof.

The invention discloses application of matrine in preparation of a product for resisting staphylococcus aureus invasion of bovine mammary epithelial cells. The invention provides an application of sophocarpidine in preparation of a product with at least one of the following functions: A1) preventing and treating animal infectious diseases; a2) anti-staphylococcus aureus or methicillin-resistant staphylococcus aureus; a3) reducing the adhesion of staphylococcus aureus or methicillin-resistant staphylococcus aureus to mammary gland cells or tissues; a4) the invasion of staphylococcus aureus or methicillin-resistant staphylococcus aureus to mammary gland cells or tissues is reduced; a5) retarding apoptosis of mammary gland cells or tissues induced by staphylococcus aureus or methicillin-resistant staphylococcus aureus. The matrine can be used for preparing veterinary drugs and preventing and treating animal diseases caused by staphylococcus aureus or staphylococcus aureus drug-resistant strains MRSA.

The invention provides a traditional Chinese medicine composition for enlarging and beautifying breasts and removing stasis and a preparation method of the traditional Chinese medicine composition. The composition is prepared from the following raw materials in parts by weight: 15 to 30 parts of camellia-seed oil, 12 to 28 parts of crithmum maritimum extract, 10 to 25 parts of red clover root extract, 3 to 10 parts of rosemary leaf oil, 1 to 3 parts of jasmine essential oil, 8 to 20 parts of leuconostoc / radish root fermentation product filtrate, 6 to 18 parts of African chandelia fruit extract, 5 to 15 parts of arnica flower extract and 0.1 to 1 part of acetyl hexapeptide-7. And blood-activating and stasis-removing auxiliary raw materials and skin-care components can also be added. During preparation, oil phase-water phase separation, gradient emulsification and vacuum ripening processes are adopted; through the synergistic effect of various active components, the composition can effectively promote mammary gland cell proliferation and adipose differentiation, has the effects of resisting inflammation and improving microcirculation, has the double effects of enlarging breasts and removing stasis and blood stasis, is good in stability, and can be used for preparing cosmetics or external medicines.