12 results about "Cell lineage" patented technology

The present application relates to a kind of pluripotent stem cell co-differentiation method for generating vascularized heart organoids, the method comprises: (1) in iPSCs, obtain iEC-iPSCs by transferring exogenous gene Tet 3G and ETV2;(2) the iEC-iPSCs in step (1) are mixed with iPSCs, add culture medium I, prepare blastula after culture;(3) the blastula in step (2) is induced by using the heart organoid differentiation method combined with doxycycline, and vascularized heart organoids are prepared after differentiation culture.The present application uses Tet-on 3G inducible expression system, promotes the differentiation of part of cells in heart organoids to endothelial cell lineage by regulating the expression of ETV2 gene in part of iPSCs in blastula, and then forms vascularized heart organoids.

The application discloses a human vagina micro-ecological in-vitro model and a construction method and application thereof, and relates to the technical field of medicines.The application discloses a human vagina micro-ecological in-vitro model and a construction method and application thereof.Firstly, a mixed cell group of the vaginal epithelium containing a complete cell lineage and capable of long-term subculture is successfully established, a technical bottleneck that primary vaginal epithelial cells are difficult to expand is solved, and ideal seed cells are provided for model construction.Secondly, a highly-bionic vaginal mucosa model having a tight connection structure, rich layers and an orderly arrangement is constructed, and the physiological barrier and immune regulation functions of the vaginal mucosa are truly simulated.Finally, by introducing lactobacillus symbiosis and confirming the response to estrogen, a multi-element in-vitro research model integrating the anatomical structure of the vagina, resident flora, local immunity and endocrine regulation is constructed, and a reliable technical platform is provided for in-depth exploration of the mechanism of vaginal micro-ecological disorder and efficient screening of related drugs.

The present disclosure provides bovine animals and methods to create recipient bovine animals for spermatogenic stem cell transplantation / complementation through modulation of the NANOS2 gene. In one embodiment genome editing was used to create bovine animals with insertions or deletions (indels) that inactivate or otherwise modulate NANOS2 gene activity so that resulting male bovines lack functional germ cells yet retain functional testicular somatic cells, and bovine females are fertile. These bovine males can then be transplanted or complemented with donor cells capable of giving rise to cells of the spermatogenic lineage, or spermatogenic stem cells and used for breeding.

Provided herein are methods, compositions, systems, kits and uses for integrated, simultaneous spatial multiomic analyses of tissues and samples including chromatin accessibility epigenomic analysis, RNA expression transcriptomic analysis, cell cluster and cell lineage analysis, transcription factor motif analysis, extrachromosomal DNA analysis and mitochondrial DNA analysis.

Animal cells, notably adult fibroblasts, are advantageously reprogrammed in direct lineage reprogramming methods using defined factors to produce proliferative and multipotent induced cardiac progenitor cells (iCPC). The iCPC thus produced can be differentiated under suitable differentiation conditions to cardiac lineage cells including cardiomyocytes, smooth muscle cells, and endothelial cells, as evidenced by expression of lineage specific markers. Sets of factors effective in combination to reprogram the fibroblasts can include a set that includes some or all of 5 factors (Mespl, Baf60c, Nkx2.5, Gata4, Tbx5), a set that includes some or all of 11 factors (Mespl, Mesp2, Gata4, Gata6, Baf60c, SRF, Isll, Nkx2.5, Irx4, Tbx5, Tbx20), a set that includes some or all of 18 factors (T, Mespl, Mesp2, Tbx5, Tbx20, Isll, Gata4, Gata6, Irx4, Nkx2.5, Handl, Hand2, Tbx20, Tbx18, Tip60, Baf60c, SRF, Hey2), and a set that includes some or all of 22 factors (T, Mespl, Mesp2, Tbx5, Tbx20, Isll, Gata4, Gata6, Irx4, Nkx2.5, Hand1, Hand2, Tbx20, Tbx18, Tip60, Baf60c, SRF, Hey2, Oct4, Klf4, Sox2, L-myc).

This invention discloses a composition for the differentiation of mesenchymal stem cells (ADSCs) into keratinocytes and its application. The composition comprises: a Wnt signaling pathway activator, a TGF-β signaling pathway inhibitor, a bone morphogenetic protein pathway activator, and at least one of retinoic acid and a retinoic acid agonist. The small molecule composition provided by this invention is meticulously designed to gradually guide the fate transition of ADSCs by mimicking key signaling events during embryonic development. This strategy is not a simple compound screening, but a rational design based on a deep understanding of developmental biology, achieving precise regulation of cell lineages in vitro by reproducing the natural developmental process. The composition of this invention is not a simple superposition of components, but rather achieves cell fate transition by precisely regulating the balance of signaling pathway networks.

A normal set of cells is characterized using flow cytometry. A centroid and radius are defined for a set of clusters in an n-dimensional space corresponding to a normal maturation for a cell lineage in the normal set of cells. A test set of cells is characterized using flow cytometry and the characterization is compared to the defined set of clusters. Support Vector Machine (SVM) subroutines are employed to identify reference populations of interest by generating multidimensional boundary definitions. These boundary definitions may be used to identify reference populations to use in defining or refining a centroid line or a radius or radii defining a set of normal clusters, and to characterize and compare a test set of cells to the defined set of normal clusters.

A method of using a cell culture bioreactor for differentiating stem cells in situ in the bioreactor is provided. The method includes providing a bioreactor vessel that has a cell culture chamber with an inlet for flowing fluid into the cell culture chamber and an outlet for flowing fluid out of the cell culture chamber. The bioreactor vessel also includes a cell substrate disposed in the cell culture chamber for culturing cells thereon. The method includes seeding undifferentiated stem cells onto the cell substrate in the cell culture chamber and perfusing the cell culture chamber with a differentiation media to promote differentiation of the undifferentiated stem cells into a specified cell lineage, whereby the undifferentiated stem cells become differentiated cells.

The application relates to the biomedical technology field and discloses an endometrial carcinoma prognosis analysis system, equipment and medium, which comprises a cell typing module and a prognosis analysis module connected in sequence. The cell typing module is used for marker recognition on a to-be-detected cell sample of a target object, so that the to-be-detected cell sample is typed into multiple target cell subtypes, including one or more subtypes in tumor cell lineage typing. The tumor cell lineage typing includes a cilia type (FOXJ1), a luminal type (LCN2), a first gland type (SCGB2A1) and a second gland type (CST1). The prognosis analysis module is used for prognosis analysis according to the multiple target cell subtypes, so as to formulate a treatment plan for the target object. The beneficial effect is that the cell sample of the target object can be subjected to targeted cell typing based on expression gene differences, the accuracy of the prognosis analysis result is improved, and important reference bases are provided for clinical treatment guidance of the target object.

The application discloses a kind of fusion local topology and coordinate decoupling honeycomb cell tracking and lineage reconstruction method, belong to biological image processing and cell lineage tracking technical field.It includes: obtaining time sequence microscopic image and extracting cell segmentation mask data based on image segmentation algorithm;Adjacent frame mask is globally registered;Local topological neighborhood graph of surrounding ordering is constructed, and the coordinate decoupled feature matrix consisting of vector angle, distance ratio and area ratio is extracted;Fusion morphology and topological feature construct comprehensive cost matrix and globally optimize solution obtain tracking correlation;Based on skeleton and topological graph network extraction vectorized polygon contour;By reverse fusion and boundary morphology evolution generates virtual mother cell to establish division relationship;With neighborhood voting, conflict adjudication is carried out and lineage fault is repaired by division priority.The application realizes high robustness of automated cell tracking and lineage reconstruction in non-aligned long-interval microscopic image.

This invention discloses an integrated CRE / LOX system, comprising a binary vector backbone plasmid, a Cre-encoding gene for the cyclization recombinase, a LOX site specifically recognizable by Cre, and a reporter gene. As a biomarker gene expression platform, this integrated CRE / LOX system can generate transgenic lines for lineage tracing in specific cells in a single step by cloning the target gene promoter, without the need for secondary transformation or hybridization. This significantly reduces the experimental cycle and workload of cell lineage tracing in plants, providing an important tool for studying the development and regeneration of plant meristems and stem cells.