95 results about "Local immunity" patented technology

Illumination devices for impinging light on tissue, for example within a body cavity of a patient, to induce various biological effects are disclosed. Biological effects may include at least one of inactivating and / or inhibiting growth of one or more pathogens, upregulating a local immune response, increasing endogenous stores of nitric oxide, releasing nitric oxide from endogenous stores, and inducing an anti-inflammatory effect. Biological effects may include upregulating and downregulating inflammatory immune response molecules within a target tissue. Wavelengths of light are selected based on intended biological effects for one or more of targeted tissue types and targeted pathogens. Light treatments may provide multiple pathogenic biological effects, either with light of a single wavelength or with light having multiple wavelengths. Devices for light treatments are disclosed that provide light doses for inducing biological effects on various targeted pathogens and tissues with increased efficacy and reduced cytotoxicity.

The invention relates to the technical field of veterinary epidemic disease detection, and discloses a veterinary epidemic disease remote monitoring and intelligent diagnosis system, which comprises a multi-mode biosensor array including an implantable miniature biochip, a wearable flexible physiological monitoring patch and an environment sensing sensor cluster, the implantable miniature biochip is used for being implanted into a key tissue organ area in an animal body and collecting microscopic physiological data such as cellular metabolism signals and local immune reaction marker concentration in real time, and the wearable flexible physiological monitoring patch is used for being attached to the body surface of the animal. By means of the multi-mode biosensor array and the edge intelligent preprocessing unit, animal physiology and environment data are accurately monitored in real time. The multi-modal biosensor array covers the implantable miniature biochip, the wearable flexible physiological monitoring patch and the environment sensing sensor cluster, and can comprehensively collect in-vivo microscopic physiological data, body surface macroscopic physiological parameters and feeding environment information of animals.

The invention relates to the technical field of dental oral treatment, and discloses a repair method for treating periodontitis by using dental pulp stem cells, which comprises the following steps: S1, extracting dental pulp tissues from the oral cavity of a patient; s2, obtaining dental pulp stem cells; s3, culturing the dental pulp stem cells under specific conditions; s4, inducing the dental pulp stem cells to differentiate in the direction of osteogenesis, chondrogenesis or periodontal ligament cell formation; s5, transplanting the differentiated dental pulp stem cells to a periodontitis injury area of the patient, and using a biocompatible material; s6, applying a proper local stimulation means around the cell transplantation area; s7, by applying anti-inflammatory drugs locally or systemically, local immune response and inflammatory response are retarded, and repair and regeneration of periodontium are promoted. The problems that an existing treatment method is not lasting in effect, long in recovery period and the like are solved, the damaged periodontium is repaired through the dental pulp stem cells, the treatment effect can be remarkably improved, and the dental pulp stem cells have high clinical application value.

The invention provides in-situ forming fluid bionic hydrogel as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The invention provides an injectable GelSSO / PDA (at) SDF fluid bionic niche, after in-situ photo-crosslinking, preferential and sustained release of PDA (at) SDF NPs initiates early signal amplification, which recruits EPCs and MSCs through an SDF-1 alpha / CXCR4 axis, promotes activation of the angiogenic vascular niche through an AKT signal, and repolarizes macrophages to a regenerated M2 phenotype. Subsequently, the gradual degradation of the bionic niche triggers the stable release of SSO, and the later signal is further amplified to form an osteogenic niche with transcriptional activity, thereby maintaining the MAPK / ERK-mediated MSCs osteogenic differentiation. The fluid bionic niche can form a niche conforming to defects through photopolymerization, the local immune imbalance and endogenous progenitor cell recruitment are corrected in the early stage, then formation of new vessels and lamellar bones is promoted, and finally vascularization regeneration of diabetic skull defects is achieved.

Provided herein are cytokine-encoding nucleic acid molecules, such as recombinant expression vectors, and uses thereof to make modified tumor infiltrating lymphocytes (TILs), wherein the modified TILs include one or more immunomodulatory agents (e.g, cytokines) associated with their cell surface. The immunomodulatory agents associated with the TILs provide a localized immunostimulatory effect that can advantageously enhance TIL survival, proliferation and / or anti-tumor activity in a patient recipient. As such, the compositions and methods disclosed herein provide effective cancer therapies.

The application discloses a method for preparing cell-derived exosomes and products and applications thereof, and the method comprises the following steps: co-culturing immune-activated programmable cells with a stimulating agent with immune-activation effect, and collecting the cell-derived exosomes with immune activity by a natural secretion collection method or an artificial extrusion collection method. The application has safe and controllable local immune activation, and the immune activation strategy based on the exosomes fully overcomes the safety bottleneck of traditional live bacterial preparations in tumor treatment. By replacing live bacteria with engineered exosomes, not only potential risks such as systemic inflammatory response and infection are effectively avoided, but also the local controllability of immune stimulation signals is ensured, the precise activation and directional regulation of tumor region immune cells are realized, the tumor microenvironment is reshaped, the anti-tumor effect is strengthened, and the broad-spectrum cross-tumor potential is verified across models.

The present invention provides a method for culturing immune organoids and its application. The present invention cultures immune organoids by isolating lung-infiltrating lymph nodes and evaluates the level of mucosal immune response produced against influenza virus. By adding components such as BAFF and IL-2 to the cell culture medium and using the air-liquid interface method to three-dimensionally reconstruct immune cells derived from lung-infiltrating lymph nodes, immune organoids with a lymphoid structure are formed. These immune organoids can more realistically reflect the level of local immune response in the lungs and better retain various immune cells. At the same time, T cells are activated, enhancing their helper effect on B cells; the time for B cells to differentiate into plasma cells and mature is greatly shortened, and the efficiency of detecting and evaluating the immunogenicity of viral antigens is improved.

The invention relates to allograft tolerance without systemic immune containment. There is provided a cell genetically modified to comprise at least one mechanism for providing localized immune containment at a transplantation site when transplanted into an allogeneic host; and methods for making and using the cells. The cell comprises a set of transgenes, each transgene encoding a gene product of cytoplasm, membrane binding or topical action, and having one or more of the following functions: mitigating antigen presenting cell activation and function; mitigating leukocyte activity or cytolytic function of the aggressive graft; the cytolytic function and phagocytosis of macrophages of allograft cells are reduced; inducing apoptosis in the graft-attacking leukocytes; reducing local inflammatory proteins; and protecting from leukocyte mediated apoptosis.

The application discloses a human vagina micro-ecological in-vitro model and a construction method and application thereof, and relates to the technical field of medicines.The application discloses a human vagina micro-ecological in-vitro model and a construction method and application thereof.Firstly, a mixed cell group of the vaginal epithelium containing a complete cell lineage and capable of long-term subculture is successfully established, a technical bottleneck that primary vaginal epithelial cells are difficult to expand is solved, and ideal seed cells are provided for model construction.Secondly, a highly-bionic vaginal mucosa model having a tight connection structure, rich layers and an orderly arrangement is constructed, and the physiological barrier and immune regulation functions of the vaginal mucosa are truly simulated.Finally, by introducing lactobacillus symbiosis and confirming the response to estrogen, a multi-element in-vitro research model integrating the anatomical structure of the vagina, resident flora, local immunity and endocrine regulation is constructed, and a reliable technical platform is provided for in-depth exploration of the mechanism of vaginal micro-ecological disorder and efficient screening of related drugs.

Embodiments described herein relate to restorative solutions for segmental peripheral nerve (PN) defects using allografted PNs for stimulating PN repair. More specifically, embodiments described herein provide for localized immunosuppression (LIS) surrounding PN allografts as an alternative to systemically suppressing a patient's entire immune system. Methods include localized release of immunosuppressive (ISV) agents are contemplated in one embodiment. Methods also include localized application of immunosuppressive (ISV) regulatory T-cells (Tregs) and / or mesenchymal stomal cells in other embodiments. Hydrogel carrier materials are also described herein.

A phototherapy device is disclosed for irradiating tissues, such as those within a patient's body cavity, with light to induce various biological effects. These biological effects may include at least one of the following: inactivation and / or inhibition of the growth of one or more pathogens, upregulation of local immune responses, increase of endogenous nitric oxide reserves, release of nitric oxide from endogenous reserves, and induction of anti-inflammatory effects. The biological effects may also include upregulation and downregulation of inflammatory immune response molecules within the target tissue. The wavelength of light is selected based on the expected biological effects on one or more of the target tissue type and target pathogens. Whether using light of a single wavelength or light of multiple wavelengths, phototherapy can provide a variety of pathogenic biological effects. A device for phototherapy is disclosed that provides a light dose to induce biological effects on various target pathogens and tissues with enhanced efficacy and reduced cytotoxicity.

The invention discloses a combined medicine for preventing and treating chronic cervicitis and a preparation method thereof. The combined medicine comprises a scavenger I and a scavenger II, according to the combined medicine and the preparation method provided by the invention, comprehensive treatment is carried out by combining a mature therapy and an elimination therapy which are special for Uighur medicine, the vagina environment is changed, the local immune function is improved, and a treatment mode which is definite in curative effect, safe and low in price is provided for chronic cervicitis.

Methods and related devices for impinging light on tissue, for example within a body of a patient, to induce various biological effects are disclosed. Biological effects may include at least one of inactivating and / or inhibiting growth of one or more pathogens, upregulating a local immune response, stimulating enzymatic generation of nitric oxide to increase endogenous stores of nitric oxide, releasing nitric oxide from endogenous stores of nitric oxide, and inducing an anti-inflammatory effect. Wavelengths of light are selected based on intended biological effects for one or more of targeted tissue types and targeted pathogens. Light treatments may provide multiple pathogenic biological effects, either with light of a single wavelength or with light having multiple wavelengths. Devices and methods for light treatments are disclosed that provide light doses for inducing biological effects on various targeted pathogens and targeted tissues with increased efficacy and reduced cytotoxicity.

Methods to treat cancer in a subject comprising administering to the subject a therapeutically effective amount of T-cells of the subject having increased IRF4 polypeptide expression compared to a control are disclosed. Also disclosed are methods of increasing tumor reactivity of a T-cell by increasing IRF4 polypeptide expression, and methods to predict the likelihood that a subject having cancer will respond therapeutically to administered T-cells having increased IRF4 polypeptide expression. Also disclosed are compositions comprising a T-cell and a viral vector encoding an IRF4 polypeptide. The compositions are methods are useful for treating numerous cancers in which higher level expression of IRF4 in T-cells would be beneficial. In some embodiments, activated tumor-specific T-cells having increased IRF4 expression have greater infiltration in tumors and enhanced local immunological responses.

The invention discloses injectable hydrogel as well as a preparation method and application thereof. The injectable hydrogel is formed by self-assembling a natural plant active component, a framework material and an inorganic nano material, the natural plant active component comprises any one of puerarin, resveratrol, curcumin and quercetin; the framework material comprises any one of chitosan, gelatin, sodium alginate, hyaluronic acid and a polylactic acid-glycolic acid copolymer; the inorganic nano material comprises any one of mesoporous silica, calcium phosphate nano particles, zinc oxide nano particles and hydroxyapatite. The injectable hydrogel composed of puerarin, chitosan and mesoporous silica realizes the synergy of local immune regulation and tissue regeneration functions, and can be used for skeletal muscle defect repair.

The invention discloses a self-adaptive fluorescent immune layer quantitative detection feature extraction method and system, and relates to the technical field of fluorescent immune detection.The method comprises the steps that a fluorescent speckle image sequence is obtained, an image gradient difference value between adjacent frames is calculated, a three-dimensional interference disturbance tensor is constructed, and a main disturbance modal map is obtained through sparse principal component analysis; constructing a self-supervised triple training sample based on the main disturbance modal map, and training an image block encoder by taking a disturbance value as a pixel-level weighting factor; extracting image block features by using the trained encoder, and matching the image block features with the response feature dictionary to generate a response probability map; calculating perturbation spectrum entropies of the time sequence maps, and generating a fusion response probability map according to reciprocal weighted fusion of the perturbation spectrum entropies; and through mask screening and response integral calculation, outputting through a fitting method. According to the method, the local immune response recognition capability can be effectively enhanced, the adaptivity of feature extraction and the stability of response fusion under interference are improved, and high-precision quantification of fluorescence immunodetection is realized.

To provide parenteral nutrition formulations.SOLUTION: The present disclosure relates to multi-chamber containers and to parenteral nutrition formulations, including ready-to-use parenteral nutrition formulations reconstituted from and amino acid formulations. More specifically, the present disclosure relates to formulations comprising butyrate derivatives, specifically arginine butyrate, for use in adult or pediatric patients. The present disclosure further provides methods for reducing or preventing systemic and local inflammation in patients receiving parenteral nutrition, as well as methods for maintaining or improving their systemic and local immunity and intestinal barrier function.SELECTED DRAWING: None

Provided herein are compositions and methods for treating cancer using modified TILs, the modified TILs comprising one or more immunomodulatory agents (e.g., cytokines) associated with their cell surface. The immunomodulatory agents associated with the TILs provide a local immunostimulatory effect that can advantageously enhance TIL survival, proliferation, and / or antitumor activity in a patient recipient. Thus, the compositions and methods disclosed herein provide effective cancer therapy.

The invention discloses a bimodal nucleic acid removal system for treating an intervertebral disc degenerative disease as well as a preparation method and an application method of the bimodal nucleic acid removal system. A nucleus pulposus cell membrane vesicle is used for entrapping a small molecule mitochondrial membrane stabilizer, and the engineered nano vesicle is prepared. The preparation method comprises the following steps: mixing a polyamidoamine (PAMAM) dendritic polymer with a hyaluronic acid solution, adding the PAMAM dendritic polymer, and carrying out Schiff alkali reaction crosslinking to form hydrogel, so as to obtain the bimodal nucleic acid removal system. The hydrogel is combined with negative cfDNA released in tissue gaps through positive charges, inflammatory nucleic acid in the tissue microenvironment is efficiently removed, polarization of M1 type macrophages and release of inflammatory factors are inhibited, and the local immune microenvironment is improved. The engineered nanovesicles are used for targeted delivery of a small-molecule mitochondrial membrane stabilizer into nucleus pulposus cells, DNA leakage caused by increase of permeability of mitochondrial outer membranes is inhibited, the cell steady state is recovered, cell function degeneration is delayed, and inflammatory response is inhibited from two aspects of intracellular sources and tissue external environments through synergistic effects.

Provided herein include new antibodies reactive for canine CD3, which are shown to substantially increase activation of canine T cells; new multi-specific immune cell engager molecules based on the canine CD3 antibodies, which are shown to effectively bind canine cancer antigens and recruit T cells for killing of the cancer cells; as well as new recombinant proteins derived from canine cartilage protein fragments and hydrogels thereof. Uses of these compositions are also provided, which include therapeutic uses of antibodies and multi-specific immune cell engager molecules for treatment of cancers or other diseases in dogs, as well as uses of hydrogels for local immunotherapy delivery for reduction of post-surgical recurrence risk, reduction of cancer at surgically inoperable sites, provision of palliation, and / or substitution for surgery.

The invention relates to a traditional Chinese medicine composition for inhibiting HPV (human papilloma virus) and treating cervical precancerous lesions after conization, and belongs to the technical field of traditional Chinese medicines. The traditional Chinese medicine composition comprises the following traditional Chinese medicinal materials in parts by weight: 8-12 parts of inonotus obliquus, 6-10 parts of ganoderma applanatum, 0.5-1 part of saffron crocus, 2-3 parts of radix sophorae flavescentis, 5-8 parts of wild ganoderma lucidum, 4-8 parts of laetiporus sulphureus, 2-3 parts of ganoderma sinensis, 3-6 parts of golden cypress, 1-1.5 parts of fructus cnidii and 10-15 parts of phellinus igniarius. The traditional Chinese medicine composition has the advantages of remarkable clinical effect, natural traditional Chinese medicine components, no drug resistance, no side effect and small burden on the body of a patient, improves the local immunity of the vagina, enhances the capability of automatically removing pathogens of the body and effectively prevents the recurrence of diseases, and has remarkable progress compared with the prior art.

The invention discloses a sperm-friendly medical lubricant and a preparation method thereof. The lubricant comprises the following components: a water-based matrix, an active component, phosphate, a humectant, an osmotic pressure regulator and water, the active ingredients comprise small molecule peptidoglycan and alkaline polypeptide. The alkaline polypeptide is alkaline protein, can mildly improve the pH environment of the vagina and is beneficial to maintaining the activity of sperms, and the small molecule peptidoglycan has a good adhesion effect on vaginal epithelial cells, can be adhered to the vaginal epithelial cells and prevents the alkaline polypeptide from stimulating the vaginal inner wall; the small molecule peptidoglycan also can promote local immune regulation, enhance secretion of anti-inflammatory factors and immune response of macrophages, and improve the anti-infection ability of vagina; the composition of the two active substances can maintain sperm activity and protect and resist infection on the vagina at the same time, and is a lubricant which is friendly to the vagina and sperms.

Provided herein are anti-FN-EIIIB nanobodies, conjugates thereof, and methods of using nanobodies and conjugates thereof. Provided are anti-ECM nanobodies coupled with cytokines, which can be used to target the tumor microenvironment and stimulate a local immune response against the tumor. It is recognized herein that several parameters affect efficacy, including increasing nanobody affinity to tumor ECM, increasing expression levels of ECM targets, and increasing delivery routes (systemic or intratumoral). The increased affinity of the nanobodies described herein provides significant advantages over prior art antibodies, including the "parent NJB2" antibody.

This disclosure provides the use of nanoparticles of self-assembled acidic molecular clusters (NP-AMCs) of ammonium salts to lower the pH of skin and underlying tissue of a human or other mammal as a means of stimulating the localized immune response to a wound.

A cell genetically modified to comprise at least one mechanism for providing a local immunosuppression at a transplant site when transplanted in an allogeneic host is, and methods for making and using the same is provided. The cell comprises a set of transgenes, each transgene encoding a gene product that is cytoplasmic, membrane bound, or local acting, and whose function is one or more of: to mitigate antigen presenting cell activation and function; to mitigate graft attacking leukocyte activity or cytolytic function; to mitigate macrophage cytolytic function and phagocytosis of allograft cells; to induce apoptosis in graft attacking leukocytes; to mitigate local inflammatory proteins; and to protect against leukocyte-mediated apoptosis.

Methods for providing a vaccine composition capable of effectively inducing a systemic immune response and / or a topical immune response upon administration wherein the composition comprises a human leukocyte antigen class I (HLAI) restrictive epitope selected from the viral pathogen non-virion constituent protein (non-VIP) and thus elicits a CD8T cell response specific against viral infected cells.

The invention provides an injectable artificial bone matrix and a preparation method and application thereof.The method comprises the following steps that S1, a whole blood sample is collected and centrifuged, and a liquid plasma matrix on the upper layer, a plasma matrix albuginea layer on the middle layer and a red blood cell layer on the lower layer are obtained; s2, respectively collecting a liquid plasma matrix and a plasma matrix albuginea layer, heating the liquid plasma matrix, and cooling to obtain plasma matrix gel; s3, uniformly mixing the plasma matrix albuginea layer, the plasma matrix gel and the bone repair material to obtain the injectable artificial bone matrix. The liquid plasma matrix is heated and cooled to form the plasma matrix gel, and the plasma matrix gel is adopted to replace a conventional solid plasma matrix to be mixed with the bone repair material, so that the relative usage amount of the bone repair material and the plasma matrix can be reduced, and the cost is reduced; meanwhile, the plasma matrix albuginea layer is added, a large number of enriched immune cells contained in the plasma matrix albuginea layer are utilized, the local immune regulation function is achieved, and early-stage wound healing can be accelerated.

A method for providing a vaccine composition capable of effectively inducing a systemic immune response and / or a local immune response upon administration, wherein the composition comprises a human leukocyte antigen class I (HLA I)-restricted epitope selected from viral pathogen non-viral particle constituent proteins (non-VIPs), and thus elicits a CD8 T cell response specific for virus-infected cells.