The invention discloses a preparation method of gynecological gel. The gel is prepared from the following traditional Chinese medicines: ginseng, cortex phellodendri, catechu, radix salviae miltiorrhizae, fructus cnidii, radix paeoniae alba, herba lycopi, radix scutellariae, fructus kochiae, radix stemonae, aloe, borneol, saffron crocus, cananga odorata, geranium, radix sophorae flavescentis, folium artemisiae argyi, dragon's blood, angelica sinensis, frankincense, propolis, radix angelicae, herba epimedii and the like. The gynecological gel is prepared by setting a proper ratio of thetraditional Chinese medicines; and further, the extracting solution of the traditional Chinese medicines is used as a main effective component, so that the traditional Chinese medicine composition is noninvasive, non-irritant, hormone-free, free of any toxic and side effects and free of a microbial tolerance phenomenon. The active ingredients of traditional Chinese medicines are used for inhibiting the activity of viruses and bacteria, quickly nourishing and repairing germ cells and improving the self-secretion function and local immunity, so that the survival of viruses and bacteria is intervened, and finally, the treatment on vaginitis, cervicitis and cervical erosion is realized. The gynecological gel disclosed by the invention has a bacteriostatic effect on staphylococcus aureus, candida albicans and escherichia coli of the female pudendum.