10 results about "Local immunity" patented technology

The application discloses a human vagina micro-ecological in-vitro model and a construction method and application thereof, and relates to the technical field of medicines.The application discloses a human vagina micro-ecological in-vitro model and a construction method and application thereof.Firstly, a mixed cell group of the vaginal epithelium containing a complete cell lineage and capable of long-term subculture is successfully established, a technical bottleneck that primary vaginal epithelial cells are difficult to expand is solved, and ideal seed cells are provided for model construction.Secondly, a highly-bionic vaginal mucosa model having a tight connection structure, rich layers and an orderly arrangement is constructed, and the physiological barrier and immune regulation functions of the vaginal mucosa are truly simulated.Finally, by introducing lactobacillus symbiosis and confirming the response to estrogen, a multi-element in-vitro research model integrating the anatomical structure of the vagina, resident flora, local immunity and endocrine regulation is constructed, and a reliable technical platform is provided for in-depth exploration of the mechanism of vaginal micro-ecological disorder and efficient screening of related drugs.

A phototherapy device is disclosed for irradiating tissues, such as those within a patient's body cavity, with light to induce various biological effects. These biological effects may include at least one of the following: inactivation and / or inhibition of the growth of one or more pathogens, upregulation of local immune responses, increase of endogenous nitric oxide reserves, release of nitric oxide from endogenous reserves, and induction of anti-inflammatory effects. The biological effects may also include upregulation and downregulation of inflammatory immune response molecules within the target tissue. The wavelength of light is selected based on the expected biological effects on one or more of the target tissue type and target pathogens. Whether using light of a single wavelength or light of multiple wavelengths, phototherapy can provide a variety of pathogenic biological effects. A device for phototherapy is disclosed that provides a light dose to induce biological effects on various target pathogens and tissues with enhanced efficacy and reduced cytotoxicity.

The present application relates to the technical field of gynecological medical biomaterials, and discloses a probiotic prebiotic repair composition for endogenous regulation of vaginal microecological balance, which is composed of active ingredients and medicinal excipients; the active ingredients are composed of 500-600 parts of galactooligosaccharides, 100-120 parts of isomaltooligosaccharides, 20-30 parts of human collagen and 15-25 parts of beta-glucan; and the medicinal excipients are selected from one or more of a lactic acid-sodium lactate pH buffer system, a carbomer mucosal adhesive material and a hydroxypropyl methylcellulose sustained-release matrix. The probiotic prebiotic repair composition for endogenous regulation of vaginal microecological balance and the preparation method thereof completely avoid exogenous live bacteria, recombinant proteins and antiviral components by using galactooligosaccharides and isomaltooligosaccharides to synergistically activate native lactobacilli, human collagen to repair the mucosal barrier, beta-glucan to enhance local immunity, a lactic acid-sodium lactate buffer system to stabilize the pH environment and hydroxypropyl methylcellulose to construct a three-dimensional sustained-release matrix.

The present invention relates to bacterial probiotics useful in aquaculture. Specifically, it discloses a selection of non-hemolytic, proteolytic, stress-tolerant bacterial strains with enhanced quorum- quenching capabilities. Moreover, the method allows detection of novel species of bacteria whose probiotic attributes could be massive for aquaculture. From the isolated bacteria, selected species were tested for their probiotic efficacy in Nile tilapia (Oreochromis niloticus), a key fish species in global finfish aquaculture. The species demonstrated significant efficacy in improving the health and growth performance of Nile tilapia juveniles. Specific growth rates and weight gain, and improved feed conversion ratios without adverse effects on fish health were observed. The gut microbiome was positively influenced by promoting beneficial bacteria while reducing harmful (opportunistic) species, leading to improved immune modulation. Survival rates were significantly boosted during a pathogenic challenge, and key immune genes were upregulated, indicating stronger local immune defenses. These findings show that the selected probiotic species offer substantial benefits for growth, feed efficiency, and disease resistance, emphasizing their potential for widespread application as probiotics in aquaculture.

The application relates to the technical field of orthopedic clinical repair, and particularly discloses a piezoelectric driving layered bionic support for repairing diabetic bone defects as well as a preparation method and application thereof. The support is a double-layer bionic structure, and the support comprises an upper-layer piezoelectric bionic periosteum simulating a periosteum and a lower-layer conductive filling hydrogel simulating a defect filling matrix. The upper-layer piezoelectric bionic periosteum is a nanofiber membrane, and the lower-layer conductive filling hydrogel is a composite three-dimensional porous hydrogel. The scheme designs a kind of physical hierarchical structure capable of simulating bone tissue, and the composite bionic support can conduct surface electrical signals to the deep part of the defect, and can synergistically regulate local immune metabolism and angiogenesis. The physical hierarchical structure material of the support exhibits excellent mechanical compressive strength, low impedance conductivity, and ultrasound-responsive on-demand degradation and drug release behavior adapted to the bone tissue healing period, and has extremely high clinical conversion potential.

This invention discloses a gynecological antibacterial hydrogel, its preparation method, and its application. The hydrogel comprises a water extract of traditional Chinese medicines including Verbena officinalis, Salvia miltiorrhiza, Portulaca oleracea, Sophora flavescens, Viola yedoensis, Phellodendron chinense, and Artemisia argyi, as well as lactic acid, chitosan, and water. The preparation involves a water extraction and alcohol precipitation purification process to obtain the herbal extract, which is then mixed with a chitosan-lactic acid swelling matrix, adjusted to pH 3.8-4.4, and then filled and sterilized. Based on the principles of traditional Chinese medicine (TCM) formulation and combined with modern pharmaceutical technology, this invention's gel possesses the effects of clearing heat and drying dampness, detoxifying and killing parasites, and promoting blood circulation and removing blood stasis. It exhibits strong inhibitory activity against common gynecological pathogens, restores the acidic environment of the vagina, regulates local immunity and microenvironment, has an adjunctive therapeutic effect on HPV infection, is non-irritating to the vaginal mucosa, and has high safety and good stability.

The application discloses a preparation method and application of a metal organic framework composite coating of active molecules loaded on a zinc alloy surface, and belongs to the technical field of biomedically degradable metal materials. The preparation method of the organic metal framework composite coating comprises the following steps: S1, preparing a ZnO nanostructure transition layer; S2, in-situ generating a metal organic framework coating induced by 2,5-dihydroxyterephthalic acid; and S3, preparing a metal organic framework composite coating loaded with deferoxamine. The application can effectively slow down the degradation rate of a zinc alloy substrate containing Mg, Ca and Sr, and makes the zinc alloy substrate containing Mg, Ca and Sr free of local corrosion, so that no toxicity is caused to cells and tissues around an implantation site. Slow release of the loaded deferoxamine can significantly accelerate blood supply reconstruction at a fracture / bone defect site, and can balance osteogenesis and osteoclastogenesis. The application solves the problems of uncontrollable degradation and lack of regulation on local immune inflammation and osteogenesis-osteoclastogenesis balance.

The application provides a method for constructing a local inflammation model of an experimental animal brain, which comprises the following steps: anesthetizing the experimental animal and fixing the experimental animal on a positioning device; injecting an immunomodulator into a target region according to the coordinates of the target region by using a puncture tool; controlling the injection speed and dose of the immunomodulator by using a micro-infusion device; keeping the puncture needle in the body for a period of time after injection to reduce the backflow of the liquid medicine; verifying the local immune activation by quantitatively detecting the expression change of an inflammation marker in the target region, and synchronously detecting the expression level of the inflammation marker in a peripheral medium to exclude a systemic immune response. The application can effectively solve the problem that the peripheral inflammation effect is difficult to distinguish in the research of brain diseases caused by systemic administration in the prior art, and can be applied to the research of the local immune activation effect of diseases such as Alzheimer's disease, ischemic stroke and glioblastoma, and the evaluation of the efficacy and mechanism of STING agonists in these diseases.

This invention provides a nanomaterial for relieving inflammation and pain in tendinopathy and its preparation method, relating to the field of biomedical technology. The nanomaterial comprises decellularized tendon DTM and hybrid membrane vesicles (MSM) of macrophages and nerve cells. The aforementioned nanomaterial for relieving inflammation and pain in tendinopathy includes decellularized tendon DTM and hybrid membrane vesicles (MSM) of macrophages and nerve cells. Combining these two components yields DTM-MSM, which regulates the proliferation and differentiation of tendon stem cells, modulates the local immune microenvironment, and relieves pain. It can improve inflammation and pain in tendinopathy and accelerate the repair of damaged tendons. In other words, DTM-MSM can not only alleviate the pathophysiological changes in tendinopathy but also rapidly improve the main clinical symptoms of tendinopathy, such as pain, swelling, and functional limitation, thus overcoming the current shortcomings of tendinopathy treatments, including slow onset of action, poor compliance, significant side effects, and unclear efficacy.

This invention relates to the field of biopharmaceutical technology, specifically to a recombinant pentamer protein, polynucleotide, expression vector, host cell, preparation method, and application. The fusion protein is formed by fusing LTB or its immunogenic functional variant with the pentamer domain of COMP via a flexible linker peptide. This invention constructs the gene of this fusion protein in a eukaryotic expression vector, transfects it into CHO-K1 cells, obtains stable high-expression cell lines through pressure selection, and performs efficient secretory expression using serum-free suspension culture. The resulting protein forms a pentamer under non-reducing conditions. The sIgA level in mouse nasal lavage fluid is significantly higher than that of the antigen alone, confirming its effective activation of local immunity in the respiratory mucosa. This invention utilizes the CHO expression system to achieve large-scale preparation of structurally correct, homogeneous, and endotoxin-free LTB pentamers, which has significant application value in the development of mucosal vaccines, targeted delivery, and diagnostic reagents.