14 results about "Nile tilapia" patented technology

The invention relates to the technical field of biology, in particular to a tilapia mossambica streptococcus agalactiae resistance related KASP molecular marker and application thereof. According to the invention, two SNP (Single Nucleotide Polymorphism) loci, namely SNP-1 and SNP-2, which are remarkably associated with resistance are screened based on an intron region of an EGFRX2 gene of tilapia mossambica, a typing method of the loci is developed through a KASP technology, and relevance between the genotype of the SNP-1 and a resistance phenotype is verified. Furthermore, streptococcus agalactiae resistance dominant diplotype individuals are screened in combination with double SNP loci, and the survival rate of a challenge experiment is remarkably improved. The detection kit provided by the invention can realize high-throughput and high-precision typing, is suitable for tilapia mossambica resistance genotype identification and molecular marker-assisted breeding, provides an efficient genetic improvement tool for tilapia mossambica disease-resistant breeding, and is beneficial to reduction of breeding disease loss and reduction of antibiotic dependence.

The present invention relates to the effect of promoting muscle development and growth through inhibition of the expression of MRF4. According to the present invention, MRF4 isolated and identified from skeletal muscles of Nile tilapia showed a strong association with MyoG and MEF2 proteins, and the expression of MyoG and MEF2 was found to significantly increase when the MRF4 gene was disrupted through CRISPR / Cas9 gene editing, and thus the present invention has the effect of being able to be used for the promotion of muscle development or the treatment of muscle diseases through the inhibition of the MRF4 gene.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker for identifying different geographical populations of Nile tilapia and application of the SNP molecular marker, and belongs to the technical field of molecular markers. The invention provides three SNP (Single Nucleotide Polymorphism) molecular markers. In a reference genome (GCF001858045.2 version), a C / T site (marked as SNP chr4-17660871) exists at the 17660871 site of a fourth chromosome, an A / C site (marked as SNP chr7-30426048) exists at the 30426048 site of a seventh chromosome, and an A / G site (marked as SNP chr16-3192004) exists at the 3192004 site of a sixteenth chromosome. As a detection target, the SNP molecular marker can effectively distinguish geographical populations of an Alexander line, an Ismelia line and a Seneigarg line of Nile tilapia. According to the method, stable, specific and accurate germplasm resource identification can be realized.

This invention provides primers, a detection kit, and applications for identifying tilapia, belonging to the field of molecular biology. The invention provides primers for identifying tilapia species, enabling rapid, efficient, and accurate identification of Nile tilapia (Oreochromis niloticus), Oreochromis aureus, and Oreochromis spp., providing a scientific basis for tilapia germplasm resource conservation and species identification.

The present invention relates to bacterial probiotics useful in aquaculture. Specifically, it discloses a selection of non-hemolytic, proteolytic, stress-tolerant bacterial strains with enhanced quorum- quenching capabilities. Moreover, the method allows detection of novel species of bacteria whose probiotic attributes could be massive for aquaculture. From the isolated bacteria, selected species were tested for their probiotic efficacy in Nile tilapia (Oreochromis niloticus), a key fish species in global finfish aquaculture. The species demonstrated significant efficacy in improving the health and growth performance of Nile tilapia juveniles. Specific growth rates and weight gain, and improved feed conversion ratios without adverse effects on fish health were observed. The gut microbiome was positively influenced by promoting beneficial bacteria while reducing harmful (opportunistic) species, leading to improved immune modulation. Survival rates were significantly boosted during a pathogenic challenge, and key immune genes were upregulated, indicating stronger local immune defenses. These findings show that the selected probiotic species offer substantial benefits for growth, feed efficiency, and disease resistance, emphasizing their potential for widespread application as probiotics in aquaculture.

The present invention discloses a method for preparing a eukaryotic expression vector of Nile tilapia CD36 protein and its application. The method comprises extracting RNA from Nile tilapia intestinal tissue and synthesizing cDNA; performing PCR amplification on the coding sequence fragment of the Nile tilapia CD36 gene to obtain the Nile tilapia CD36 protein gene; and then constructing a eukaryotic expression vector pEGFP-N1-CD36 of the Nile tilapia CD36 protein. The eukaryotic expression vector of the nucleotide sequence of the Nile tilapia CD36 protein prepared by the present invention can be used to prepare a product that improves the anti-streptococcal immunity of tilapia. The preparation of the product is simple, easy to operate, and low in cost. The present invention enhances the anti-streptococcal ability of tilapia by overexpressing the Nile tilapia CD36 protein in tilapia, thereby increasing the survival rate of tilapia infected with Streptococcus agalactiae by 60%. In addition, overexpressing the Nile tilapia CD36 protein in tilapia can also reduce the Streptococcus agalactiae load in tilapia. Therefore, it can be seen that the technical solution of the present invention can effectively induce cellular immunity in tilapia.

The present invention relates to the development of a microencapsulated vaccine containing inactivated Streptococcus agalactiae, together with the adjuvants beta glucan and mineral oil, to be administered orally to fish together with feed in pelletised form or coated with the vaccine. In addition, the invention relates to the use of this vaccine to immunise fish, more specifically Nile tilapia (Oreochromis niloticus) to activate both innate and adaptive (humoral and cellular) intestinal mucosal immunity and protect tilapia against streptococcosis. Such a vaccine can be applied in intensive fishing production systems, with the aim of reducing fish mortality, reducing the use of antimicrobials and not causing environmental damage, being easy to administer and control, reducing fish handling, and being suitable for immunising large numbers of animals at the same time without stress and at low cost.

The invention discloses Chinese herbal medicine sappan wood for treating tilapia bacterial meningitis, application and an application method, the Chinese herbal medicine sappan wood effectively becomes brazilin, the brazilin is a homoisoflavone compound, and the molecular formula of the brazilin is C16H14O5. The application includes that the compound has an inhibiting effect on tilapia mossambica source streptococcus agalactiae and can penetrate through a tilapia mossambica blood brain barrier. The application method comprises the steps of soaking treatment, pouring treatment and mixing treatment. The hematoxylon is selected as the medicine for treating the nile tilapia bacterial meningitis for the first time and has the effects of lowering the expression level of inflammatory factors of the nile tilapia bacterial meningitis and improving the survival rate of the nile tilapia, and the Chinese herbal medicine hematoxylon used in the invention is simple in use mode and low in price and has very strong clinical transformation significance.

The invention discloses host-derived spermaceti capable of targeted enhancement of fish T cell immunity and application of spermaceti, and belongs to the technical field of microbial addition. According to the present invention, the cetobacter sobolifera strain is cetobacter sobolifera SH518, and the nucleotide sequence of the cetobacter sobolifera strain is represented by SEQ ID NO: 1; the invention also discloses an animal feed / probiotic feed. The animal feed / probiotic feed comprises a common feed and the spermaceti soxhlet; wherein no less than 107 CFU of the spermaceti is added into each gram of the common feed. According to the invention, the spermaceti soxhlet is added into the fish feed, so that the resistance of T cells of Nile tilapia to Edwardsiella fish killing infection is enhanced; the fish host-derived animal feed / probiotic feed provided by the invention is simple in preparation method, simple and convenient to operate, relatively low in cost and easy to popularize. The method has a wide application prospect.

The invention belongs to the technical field of aquatic animal breeding, and discloses a growth evaluation method of a hybrid offspring of Zhuangluo No.1 and Guiafil Tilapia, which comprises the following steps: carrying out cross breeding on a 'Guiafil No.1' Tilapia strain with obvious growth advantages and a disease-resistant new variety 'Zhuangluo No.1', comparing growth character indexes of offspring, and comparing the growth character indexes of the offspring in countable characters. The spine number of the dorsal fins of the four groups of offspring is 14-17, the number of the dorsal fins is 12-14, the spine number of the dorsal fins of the Nile tilapia is 14-18, and the number of the dorsal fins of the Nile tilapia is 10-14, which indicates that the four groups of offspring are closer to the Nile tilapia. In measurable characters, the body type of the hybrid progeny is between that of two parents, and the ZG group is closer to that of the female parent GG group. After 90 days, the average weight of the ZG group and the GZ group is different from that of the GG group, but the difference is not obvious (P is greater than 0.05), but the standard deviation of the ZG group is greater than that of the GZ group and the GG group. The survival rates of the four groups of filial generations are ZZ > ZG > GG > GZ under the condition that the whole group is not infected with streptococcus, and the yield is ZG > GZ > GG > ZZ.

The invention relates to the technical field of aquaculture, in particular to nile tilapia hepcidin as well as a preparation method and application thereof and a bacteriostatic agent. The amino acid sequence of the nile tilapia hepcidin is as shown in SEQ ID No.1, and the nile tilapia hepcidin can inhibit streptococcus agalactiae and / or aeromonas hydrophila. According to the method, the nile tilapia hepcidin can be simply, conveniently and efficiently purified, in-vitro experiments prove that the nile tilapia hepcidin can inhibit proliferation of pathogenic bacteria, and in-vivo experiment research results prove that the hepcidin can remarkably improve the streptococcus infection resistance of tilapia.

The invention provides an application of Klebsormidum sp.GXU-A8 as an aquatic animal feed additive, and belongs to the technical field of aquatic animal feeds. According to the invention, the krill GXU-A8 is continuously cultured for 10 days under the condition of 0.75 g / L nitrogen, so that the krill cells are obtained. By adding 5% of GXU-A8 and 10% of GXU-A8 into the feed, the FBW, WGR and SGR of the tilapia can be remarkably improved, and the levels of VSI and serum ALT, AST, TG, CHO and LDL are reduced; the GSH-PX activity of the nile tilapia liver is improved, the MDA content is reduced, the liver is protected, the digestion function is optimized, and the enteric microorganism richness is increased. The GXU-A8 synergistically improves the health level of tilapia mossambica by regulating and controlling lipid metabolism and immune regulation and control pathways of nile tilapia mossambica, relieving oxidative stress, enhancing iron metabolism and antibacterial immunity, optimizing lipid metabolism and enhancing intestinal barrier, nutrient absorption and immunocompetence.

The invention provides application of PTX3 protein in tilapia mossambica antibacterial infection, and belongs to the technical field of aquaculture. Specific immunoaffinity purification and glycosidase treatment are combined to prove that the nile tilapia PTX3 protein has functional glycosylation modification, and the modification characteristic is directly associated with the immunoprotection activity of the nile tilapia PTX3 protein; an in-vivo gene knock-down model is successfully constructed by utilizing targeting siRNA, so that the pathogen removal capability after infection is remarkably reduced, the pathological damage of tissues is aggravated, and the necessity of PTX3 in resisting streptococcus agalactiae infection is confirmed; furthermore, a foreign protein refilling experiment proves that the immune failure state caused by gene defects can be effectively reversed by directly providing the PTX3 protein, the survival rate of a host is remarkably improved, a foundation is laid for developing a fish bacterial disease treatment strategy based on the protein, and the method has a wide application prospect. Meanwhile, the established gene interference model provides a tool platform for quantitative evaluation for fish immune mechanism research.

The application discloses a method for creating a new strain of fast-growing Nile tilapia, and belongs to the technical field of fish breeding. The method comprises the following steps: (1) taking a fragment as shown in SEQ ID NO. 1 in the third exon of the pomc gene as a gRNA target sequence; (2) designing and synthesizing a gRNA primer sequence; (3) obtaining a gRNA fragment by transcription and purification after amplification of the gRNA primer sequence and a gRNA plasmid; (4) transferring the gRNA fragment into fertilized eggs of the Nile tilapia through gene editing to obtain F0 generation positive fish; and (5) crossing the F0 generation positive male fish with wild-type female fish, screening F1 generation parent fish, and cultivating the F1 generation parent fish to obtain F2 generation Nile tilapia with homozygous deletion mutation. The application establishes a pomc homozygous knockout strain in the Nile tilapia for the first time, and the new fast-growing strain is a complete gene function loss model, has lower cost and higher efficiency, and the phenotype can be stably inherited.