225 results about "Bacterial disease" patented technology

The invention relates to a microbial agent prepared based on a new strain of bacillus velezensis, which has the function of broad-spectrum antagonism of common pathogenic bacteria of aquatic products and can effectively prevent and / or treat bacterial diseases and / or fungal diseases of aquatic organisms including crustaceans and fishes. Meanwhile, the content of nitrite and ammonia nitrogen in aquaculture water can be effectively reduced, and the usage amount and residual quantity of antibiotics and chemical drugs in aquaculture are reduced.

The invention belongs to the field of molecular biology, and particularly relates to application of OsDOF3 protein or a coding gene thereof to improvement of rice broad-spectrum disease resistance and regulation of agronomic traits. According to the application, an OsDOF3 knockout mutant and an overexpression transgenic line are constructed under the background of susceptible rice, resistance phenotype analysis is performed by inoculating various pathogenic bacteria (including pyricularia oryzae, rhizoctonia solani and bacterial blight), and it is proved that OsDOF3 participates in regulation and control of an immunoreaction pathway of rice to fungal diseases (rice blast and sheath blight) and bacterial diseases (bacterial blight). Meanwhile, the application also finds that after a strong promoter ubi is adopted for driving, an overexpression material of the OsDOF3 is shorter than that of a wild plant, and the OsDOF3 influences normal growth and development of rice while stimulating plant immune response. The invention provides important gene resources and molecular theoretical basis for developing a new variety of broad-spectrum disease-resistant rice.

The invention belongs to the field of prevention and control of bacterial diseases of aquatic animals, and discloses an acid-resistant broad-spectrum vibrio parahaemolyticus bacteriophage LVPP58 and application of the acid-resistant broad-spectrum vibrio parahaemolyticus bacteriophage LVPP58 in vibrio disease prevention and control, and the preservation number of the bacteriophage is CCTCC M 2025322. The bacteriophage is a broad-spectrum vibrio parahaemolyticus bacteriophage, not only can split vibrio parahaemolyticus, but also can split vibrio alginolyticus, the bacteriophage LVPP58 is stable in titer at the pH of 3-11 and the temperature of 30-60 DEG C, the bacteriophage and antibiotics are combined for use, a synergistic effect can be generated, the treatment effect is enhanced, the occurrence rate of drug resistance is reduced, and a new strategy is provided for solving the problem of bacterial drug resistance.

The invention belongs to the field of biological agriculture, and particularly relates to bacillus amyloliquefaciens as well as a fungicide and application thereof. The invention discloses bacillus amyloliquefaciens B26 which is preserved in China General Microbiological Culture Collection Center on November 6, 2024, and the preservation number is CGMCC No.32519. The strain has a broad-spectrum antibacterial effect on fungal diseases and bacterial diseases, particularly has a remarkable antagonistic effect on tobacco wildfire germs, and can be used for preparing a bacillus amyloliquefaciens strain. The bacillus amyloliquefaciens B26 is used for preventing and treating the tobacco wildfire disease, it is found that the strain has an obvious preventing and treating effect on the tobacco wildfire disease, and a fungicide of the bacillus amyloliquefaciens B26 also has a good preventing and treating effect on the tobacco wildfire disease in a pot experiment. And a new scheme is provided for plant disease prevention and treatment, especially for green prevention and treatment of tobacco wildfire.

The invention provides acinetobacter for culturing prawns in saline-alkali water and application of the acinetobacter. The preservation number of the provided acinetobacter is CGMCC (China General Microbiological Culture Collection Center) No.36066. The acinetobacter soilis strain provided by the invention has remarkable comprehensive application advantages, can still keep higher growth activity and metabolic capability in a low-temperature environment, and continuously plays a water body purification function. Ammonia nitrogen and nitrite nitrogen in a water body can be efficiently degraded, the environment of the aquatic water body is remarkably improved by promoting the nitrogen circulation process, and harm caused by nitrogen pollution is reduced. In addition, the strain can effectively inhibit and prevent reproduction of pathogenic vibrios and reduce the occurrence risk of bacterial diseases of aquatic animals. The strain is high in safety, does not cause poison or stress reaction to bred animals even under the condition of high-concentration use, and has good ecological safety and environmental friendliness.

The present invention belongs to the technical field of rapid detection of drug sensitivity for bacteria. Disclosed are an enrichment culture medium, and a preparation method therefor and the use thereof. The enrichment culture medium comprises the following raw materials in parts by mass: 15.0-20.0 parts of tryptone, 3.5-5.5 parts of peptone, 1.5-3.5 parts of glucose, 5.0-6.5 parts of yeast extract, 2.0-2.5 parts of dipotassium phosphate, 2.0-3.5 parts of disodium hydrogen phosphate, 1.0-1.5 parts of monopotassium phosphate, 2.5-7.5 parts of sodium chloride, 12.0-18.0 parts of proteose peptone, 15.0-22.0 parts of beef heart infusion powder, 8.0-12.0 parts of soya peptone, 1.0-5.0 parts of 0.1% nicotinamide adenine dinucleotide (NAD), 30-100 parts of newborn calf serum inactivated at 56°C for 30 min, 2.0-4.0 parts of mannitol, 3.0-5.0 parts of sodium pyruvate, 0.23-0.45 parts of cycloheximide, 0.8-2.0 parts of 0.05% bromothymol blue, 2.5-4.5 parts of 0.1% methylene blue, and 1000 parts of distilled water, with the pH value of 6.8-7.5. The enrichment culture medium is used to screen effective therapeutic drugs for bacterial diseases in poultry, and is convenient to use and has a single enrichment effect superior to that of respective selective enrichment broths of target bacteria. Therefore, the culture medium can realize rapid proliferation of common pathogenic bacteria in poultry.

The invention relates to paenibacillus strongly ZKSW001 as well as a fungicide and application thereof, and belongs to the technical field of microorganisms. The paenibacillus pumilus ZKSW001 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.36076, the preservation date is September 25, 2025, and the address of the preservation institution is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The invention also provides application of the paenibacillus strongly ZKSW001 in inhibition of harmful bacteria in soil, prevention and treatment of root-knot nematode and promotion of plant growth. The paenibacillus strongly ZKSW001 disclosed by the invention not only can promote the growth of crops and realize weight loss and efficiency improvement, but also can control the occurrence of soil-borne fungal and bacterial diseases, and can inhibit the growth of root-knot nematode. Chemical pesticide application can be reduced, pesticide residues are reduced, and sustainable development of agricultural economy is promoted.

The invention discloses a preparation method of a recombinant lactic acid bacteria preparation for expressing antibacterial peptide and interleukin 22 (IL-22) and application of a composite microecological preparation composed of the antibacterial peptide and the interleukin 22 in prevention and treatment of animal bacterial infection diseases. The probiotic preparation provided by the invention comprises lactic acid bacteria, antibacterial peptide and interleukin, the recombinant lactic acid bacteria preparation can be taken orally without generating toxicity to an organism, and the recombinant lactic acid bacteria preparation can be colonized in intestinal tracts and secrete the antibacterial peptide and IL-22. The antibacterial peptide secreted by the recombinant lactic acid bacteria can punch bacterial cell membranes to cause bacterial death, does not cause generation of drug-resistant bacteria, and can reduce or replace the use of antibiotics; iL-22 secreted by the recombinant lactic acid bacteria can promote the repair of damaged tissues of intestinal tracts, activate the immune system of the organism and enhance the resistance; the probiotic effect of lactic acid bacteria can regulate the balance of intestinal flora of the organism, promote nutrient absorption and enhance the immunity of the organism. The preparation provided by the invention has the advantages of innovativeness, good safety, no toxic or side effect, simple administration mode, remarkable effect and the like, can effectively reduce the use of antibiotics, and provides a new solution for preventing and treating bacterial diseases in animal husbandry.

The present invention discloses the application of coumarin-3-carboxylic acid in preventing and treating plant bacterial diseases. The coumarin-3-carboxylic acid provided by the present invention has a significant inhibitory effect on plant bacterial diseases. The plant bacterial diseases are oryzae bacterial leaf blight, oryzae bacterial leaf streak, citrus canker, cantaloupe bacterial fruit spot, tomato bacterial wilt, strawberry bacterial angular leaf spot, cabbage black rot, cabbage soft rot, potato soft rot, mango bacterial angular leaf spot, cassava bacterial wilt, cucumber bacterial angular leaf spot, kiwifruit canker and banana bacterial soft rot. The coumarin-3-carboxylic acid provided by the present invention has protective and therapeutic effects on melon bacterial fruit spot.

The invention discloses a bactericidal composition containing cuaminosulfate and benziothiazolinone, the composition contains cuaminosulfate and benziothiazolinone, and the weight part ratio of cuaminosulfate to benziothiazolinone in the composition is (1: 8)-(32: 1). The bactericidal composition containing cuaminosulfate and benziothiazolinone is used for preventing and treating bacterial diseases. According to the invention, cuaminosulfate and benziothiazolinone are compounded for use, the synergistic effect is obvious, the development of drug resistance of diseases can be delayed, the service life of the pesticide can be prolonged, the control effect of effective components of the pesticide can be obviously improved, the number of times of pesticide application is reduced, the pesticide application amount is reduced, and the environmental pressure is relieved, so that the effects of main prevention and comprehensive control are achieved; the requirement of national'zero increase 'of pesticides is met.

A fermented traditional Chinese medicine feed additive for catfish, its preparation method, and its application are disclosed, relating to the field of aquatic feed additives. The preparation method involves activating and expanding the culture of *Saccharomyces cerevisiae*, *Bacillus subtilis*, and *Lactobacillus plantarum*; pulverizing *Paeonia suffruticosa* leaves, *Smilax china*, *Scutellaria baicalensis*, *Rhus chinensis*, hawthorn, *Polygonum cuspidatum*, ginkgo, and forsythia suspensa* to obtain traditional Chinese medicine powders; preparing a solid fermentation medium using the traditional Chinese medicine powders, soybean meal powder, corn cob granules, and wheat bran; inoculating *Saccharomyces cerevisiae* seed culture and *Bacillus subtilis* seed culture for aerobic fermentation; and inoculating *Lactobacillus plantarum* seed culture for anaerobic fermentation to obtain the fermented traditional Chinese medicine feed additive. The resulting fermented traditional Chinese medicine feed additive promotes the absorption and metabolic elimination of enrofloxacin in catfish, shortens the withdrawal period, has preventive and adjunctive therapeutic effects on bacterial diseases in catfish, promotes growth, reduces feed conversion ratio, and improves survival rate.

The invention discloses an LAMP (loop-mediated isothermal amplification) detection primer group for flavobacterium psychrophilum and application of the LAMP detection primer group, and belongs to the technical field of molecular biology. The method comprises the following steps: firstly, searching a specific sequence which is completely not matched with other genomes according to a whole genome sequence of flavobacterium psychrophilum, and designing a specific LAMP detection primer group according to the specific sequence; the LAMP detection primer group comprises a pair of outer primers and a pair of inner primers, and the sequence compositions of the outer primers and the inner primers are respectively shown as SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 and SEQ ID NO: 4. The primer group is used for constant-temperature fluorescent LAMP detection of flavobacterium psychrophilum, and the result shows that the LAMP detection primer group designed by the invention has a good amplification effect on detection of target flavobacterium psychrophilum, and has the advantages of strong specificity, high sensitivity, convenience and rapidness in operation and the like when being used for detecting flavobacterium psychrophilum. The method is suitable for early screening, accurate diagnosis and epidemic situation detection of freshwater fish bacterial diseases caused by flavobacterium psychrophilum.

The application provides a compound microbial agent for preventing and treating bacterial diseases of fruits and vegetables and a preparation method thereof, wherein the compound microbial agent comprises Brevibacillus brevis, Pseudomonas chlororaphis and Streptomyces; wherein the ratio of the viable bacterial count of the Brevibacillus brevis, the Pseudomonas chlororaphis and the Streptomyces is (1-3):(2-4):(1-2). The Brevibacillus brevis directly inhibits bacteria by secreting antibacterial peptides, the Pseudomonas chlororaphis competes for ecological niches through iron carriers, and the Streptomyces produces antibiotics, so that multiple strains form a "bacteriostasis-competition-immune activation" multiple mechanism, thereby achieving efficient prevention and treatment of bacterial diseases of fruits and vegetables.

The invention belongs to the field of molecular biology, and particularly relates to application of an OsNiR1 gene to improvement of rice broad-spectrum disease resistance and improvement of rice agronomic traits. The invention provides an application of an OsNiR1 gene, and the application comprises an application in preventing and treating plant diseases and / or enhancing the resistance of plants to pathogenic bacteria, and / or an application in improving agronomic traits of the plants. The amino acid sequence coded by the OsNiR1 gene is as shown in SEQ ID NO. 1, and the CDS sequence of the OsNiR1 gene is as shown in SEQ ID NO. 2. The application proves that OsNiR1 participates in regulating and controlling the immune response pathway of rice to fungal disease rice blast, fungal disease banded sclerotial blight and bacterial disease bacterial leaf blight. Meanwhile, after the strong promoter ubi is adopted for driving, an overexpression material of the OsNiR1 is higher and stronger than a wild plant type, and the tiller number is increased. The invention provides important gene resources and molecular theoretical basis for developing a new variety of broad-spectrum disease-resistant rice.

The invention belongs to the technical field of biological medicine, and particularly relates to a sophora flower extract for inhibiting formation of an oral biofilm and application thereof. According to the method, the sophora flower extract obtained after alcohol extraction is subjected to specific macroporous resin column elution conditions, the sophora flower extract obtained through enrichment has a very excellent effect of inhibiting oral biofilm formation, and further elution is carried out under specific silica gel column conditions, so that the effect of the obtained sophora flower extract is further improved. The inhibition rate of the obtained sophora flower extract is obviously higher than that of sophora flower extracts prepared by traditional alcohol extraction or other processes, and the sophora flower extract has important application value in preparation of products for inhibiting and / or killing oral microorganisms or biological membranes thereof, and can be used for preventing and / or treating dental bacterial diseases including decayed teeth.

The present document discloses a method for producing a bacterial vaccine composition against bacterial diseases in animals, such as tetrapods or fish, wherein said bacterial vaccine composition comprises inactivated bacteria of the bacterium causing said bacterial diseases. The method involves exposing the bacteria to different growth conditions and then preparing a bacterial vaccine composition where the bacteria are exposed to different growth conditions. With vaccines prepared by the present method, bacterial diseases that previously had not been able to be cured could be treated or the effect of the treatment was more long-lasting.

The application provides a new bacteriophage for preventing and treating vibrio diseases of aquatic animals and application thereof, the bacteriophage is preserved in China Center for Type Culture Collection on September 4, 2020, and the preservation number is CCTCC M 2020465. The bacteriophage is a new species of bacteriophage of the Caudovirales class and Queuovirinae subfamily, the whole genome of the bacteriophage is OP331229.1 in the NCBI official website GeneBank. The bacteriophage has a wide phage spectrum, can crack 6 different species and genera of vibrio at the same time, can crack 45 strains of Vibrio parahaemolyticus, 22 strains of Vibrio harveyi, 17 strains of Vibrio alginolyticus, 13 strains of Vibrio vulnificus, 11 strains of Vibrio campbellii and 9 strains of Vibrio cholerae at the same time, can more effectively solve the problem of multiple bacterial disease infections in aquaculture, and can be used as a microecological preparation for preventing and treating vibrio diseases such as Vibrio parahaemolyticus, Vibrio harveyi and Vibrio alginolyticus of aquatic animals.

The present application relates to a kind of fungicide composition;Also related to the application of the fungicide composition in the field of agriculture, forestry or horticulture in preventing or preventing plant pathogenic fungi from infecting plants.The fungicide composition comprises active ingredient A and active ingredient B, the active ingredient A is formula (I) compound, the active ingredient B is bronopol, kasugamycin, the mass ratio of the active ingredient A and active ingredient B is 1:24-60:1.The fungicide composition of the present application has good control effect on bacterial diseases, especially on cucumber bacterial angular spot disease, and shows significant synergistic effect, which can effectively reduce the amount of drug, reduce agricultural input cost, and delay the resistance of plant pathogenic fungi.

The invention relates to the technical field of pesticide compounding, and discloses a bactericidal compound composition containing quinolone compounds and application thereof. The composition comprises effective active components, the effective active components comprise a compound I and a compound II, the compound I is 7-chloro-1-cyclopropyl-6-fluoro-4-oxo-1, 4-dihydroquinoline-3-carboxylic acid-2-methoxyethyl ester, and the compound II is other compounds with bacterial killing activity, and the compound I and the compound II are selected from the group consisting of 7-chloro-1-cyclopropyl-6-fluoro-4-oxo-1, 4-dihydroquinoline-3-carboxylic acid-2-methoxyethyl ester and 7-chloro-1-cyclopropyl-6-fluoro-4-oxo-1, 4-dihydroquinoline-3-carboxylic acid-2-methoxyethyl ester. The bactericidal compound composition disclosed by the invention has the following advantages: (1) the bactericidal compound composition has a synergistic effect and can improve the prevention and treatment effect; (2) the bactericidal spectrum is expanded, field diseases are mixed, and the effect on bacterial diseases is stronger; (3) the two effective components are mixed for use, so that the drug resistance of pathogenic bacteria can be delayed; and (4) the application amount is reduced, the use frequency is reduced, and the use cost is reduced.

The application discloses a pesticide composition containing zhongshengmycin and kanamycin and application thereof, and belongs to the technical field of pesticides. Effective components of the pesticide composition are zhongshengmycin and kanamycin. The mass ratio of the kanamycin to the zhongshengmycin is 1:1-9. The two effective components of the application have different action sites for killing pathogenic bacteria, and can effectively alleviate the generation of drug resistance of the pathogenic bacteria. After the zhongshengmycin and the kanamycin are compounded, synergistic effect is obvious, the control effect of the pesticide on bacterial diseases, such as citrus canker, is improved, the pesticide dosage can be reduced, and agricultural residues are reduced. Meanwhile, the prepared soluble granules do not use any organic solvent, are safe to crops, and have no residues and pollution to the environment.

This invention relates to the field of agricultural pest control, and particularly to a compound nanogel sustained-release agent, its preparation method, and its application. This invention provides a compound nanogel sustained-release agent comprising bromonitol, a synergist, a carrier material, and a cross-linking agent, wherein the synergist includes one or more of kasugamycin, amino oligosaccharides, thiazolium zinc, and copper hydroxide. This invention prepares a nanogel sustained-release agent by compounding bromonitol and the synergist, which has significant preventive effects against plant bacterial diseases such as bacterial wilt, bacterial angular leaf spot, canker, and soft rot. Furthermore, the compound nanogel sustained-release agent of this invention has small particle size, stable physicochemical properties under cold and heat, good dispersibility in water, no particle agglomeration, no particle size increase, a decomposition rate of less than 5%, a long-lasting effect, good safety, is not prone to inducing drug resistance, requires a small dosage, and simultaneously increases yield and efficiency.

The invention relates to bacillus safensis AHAU-WU410 and application of the bacillus safensis AHAU-WU410 in prevention and treatment of corn bacterial wilt and promotion of corn plant growth, and belongs to the technical field of biological prevention and treatment, a living body pure culture of the bacillus safensis AHAU-WU410 is preserved in China Center for Type Culture Collection in Wuhan University, the preservation date is August 4, 2025, the preservation number is CCTCC NO: M 20251769, and the preservation number is CCTCC NO: M 20251769. The bacillus safensis AHAU-WU410 has a remarkable antagonistic effect on pathogenic bacteria pantoea stutzeri of the corn bacterial wilt, and can promote the growth of corn plants by secreting IAA (Indoleacetic Acid) and playing a phosphate solubilizing role. The bacillus safensis AHAU-WU410 is screened and identified, the control effect, the biocontrol mechanism and the growth promoting function of the biocontrol bacterium are verified and analyzed, and a new direction and a theoretical basis are provided for developing a green biocontrol bacterium agent related to corn bacterial disease control.

The present application belongs to the field of agricultural fungicides, and particularly relates to a fungicidal composition and application thereof.The composition comprises two active components A and B, the weight ratio of the active component A to the active component B is 1:99-99:1; the active component A is one or more of substituted pyrazole compounds containing pyrimidine or salts thereof, and the active component B is selected from one fungicide.The fungicidal composition is particularly suitable for preventing and treating various plant pathogenic fungi and bacterial diseases.

The application discloses a breeding method for improving the quality of Sebastes schlegelii fry, and comprises the following steps: collecting parent fish in a Sebastes schlegelii original seed field, artificially cultivating the parent fish in a net cage at sea, and performing nutrition rejuvenation on the parent fish; obtaining offspring fry by using an artificial fry squeezing method; controlling reasonable culture density according to different fry specifications; screening and using natural plant active ingredients and common micro-ecological preparations which are easy to absorb; avoiding disinfection and drug use; and controlling water flow rate in different growth stages. The method integrates nutrition rejuvenation, artificial fry production, pollution-free water quality regulation and fry behavior state regulation, solves problems such as poor parent germplasm, dead fry or insufficient activity of fry, slow growth and poor stress resistance in the process of fry breeding, has strong controllability in the operation period, has high survival rate of fry, has remarkable growth promotion and immunity effect, has good prevention and treatment effect on bacterial diseases, realizes pollution-free fry breeding, can reduce disease occurrence and mortality, reduces the feed conversion ratio, and effectively improves the economic benefit of breeding.

The invention relates to application of a dazomet liquid preparation in soil treatment in the field of pesticides. The liquid preparation can be used for preventing and treating true and bacterial diseases, nematodes and weeds of crops, and has a remarkable synergistic effect on soil-borne bacteria, fungal diseases, root-knot nematodes and field weeds. The pesticide application mode can be selected from one or more of drip irrigation, flushing application, mist spraying and soil layer treatment, and the pesticide can be directly used or used after being diluted for multiple times during pesticide application. Besides, the core advantage of the application is that the application limitation of the traditional dazomet preparation is broken through, and the dazomet preparation is suitable for wide regions, especially suitable for low-temperature regions and environments with the soil temperature being lower than 10 DEG C, and is especially preferably applied to rice seedling raising fields in low-temperature regions; multiple operation modes such as soil mixing, pulping and rice hull mixing can be adopted in a low-temperature scene, the operation is convenient and fast, the actual field requirements are met, and the environmental adaptability and the application flexibility of the dazomet preparation are remarkably improved.

The invention discloses bacillus amyloliquefaciens capable of resisting plant pathogenic bacteria and application of the bacillus amyloliquefaciens, and belongs to the technical field of microorganisms, the bacillus amyloliquefaciens is classified and named as bacillus amyloliquefaciens KT-7, the bacillus amyloliquefaciens KT-7 is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on June 9, 2020, and the address is No.66, Courtyard No.100, Xianlie Middle Road, Guangzhou City, Guangdong Province; the preservation number of the strain is GDMCC No: 61051. The bacillus amyloliquefaciens provided by the invention has a growth inhibition effect on plant pathogenic bacteria, and particularly has a good control effect on bacterial diseases such as citrus canker and tomato bacterial wilt.

The invention belongs to the technical field of prevention and control of plant bacterial diseases, and particularly relates to an amide derivative containing benzoxazole sulfone and a preparation method and application thereof.The amide compound containing benzoxazole sulfone has good antibacterial activity on plant pathogenic bacteria of rice bacterial leaf blight, rice bacterial streak and citrus canker, and can be used for preventing and controlling bacterial diseases of plants such as rice bacterial leaf blight, rice bacterial streak and citrus canker. The method has the advantages of simple preparation process, excellent reaction yield and low raw material cost; the structural formula of the derivative is shown as I,

The invention belongs to the technical field of prevention and control of diseases of aquatic animals, and discloses application of a biscoumarin derivative in preparation of a medicine for preventing and treating nocardia seriolae diseases of the aquatic animals. The bishydroxycoumarin derivative is 3, 3 '-(ethane-1, 1-diyl) bis (4-hydroxycoumarin), and can be used as an active ingredient for preparing a medicine for preventing and treating bacterial diseases of aquatic animals, and preferably, a mixed feeding use form is adopted. Experiments prove that the minimum in-vitro inhibitory concentration of the derivative to nocardia seriolae is about 3.125 mu g / mL, the survival rate of the derivative in 28 days is 50-56% when the derivative is mixed with largemouth bass infected with the nocardia seriolae at the concentrations of 200 mg / kg and 2000 mg / kg, and the derivative has no obvious toxicity to the largemouth bass within the feed concentration range of 100-3200 mg / kg, and can effectively solve the problems of poor curative effect, residue, drug resistance and the like of traditional antibiotics.

The Bacillus velezensis M173 was deposited with the Guangdong Microbial Culture Collection Center with a deposit number of GDMCC No. 61434. After being prepared into a seed-coating agent, Bacillus velezensis M173 can promote the growth and germination of plants and significantly improve the control effect on pathogens. Moreover, Bacillus velezensis M173 can relieve the condition of dead seedlings of crops at seedling stage after being applied to the crops. In particular, both pot experiments and field experiments prove that the Bacillus velezensis M173 of the present invention has a significant control effect on fungal and bacterial diseases, in particular, bacterial wilt and stem rot. Therefore, the Bacillus velezensis M173 of the present invention has broad application prospects.

The application provides a Cibotium barometz root endophytic fungus Pseudosperma yunnanense JMG086 and a separation method and application thereof belong to the technical field of bacterial disease control. The Cibotium barometz root endophytic fungus Pseudosperma yunnanense JMG086 is named as Pseudosperma yunnanense , and the preservation number is CCTCC NO: M 20251771. It is found through experiments that the Cibotium barometz root endophytic fungus Pseudosperma yunnanense JMG086 liquid fermentation broth extract has a strong inhibitory effect on the growth of Agrobacterium tumefaciens, Pectobacterium carotovorum and Ralstonia solanacearum, and can effectively prevent and control plant diseases.