The present invention discloses a
molecular marker tightly linked to a resistance locus to
bacterial leaf streak disease in rice and its application. The inventors hybridized HD10 (♂), a source resistant to rice leaf streak
disease throughout the entire growth period, with 9311 (♀), a variety highly susceptible to rice leaf streak
disease throughout the entire growth period. Using HD10 as the donor parent and 9311 as the recurrent parent, they obtained BC1F1 and self-pollinated the resulting BC1F2
population. The
population was then subjected to resistance identification, phenotypic and resistance
gene linkage analysis, and fine mapping of the resistance locus. The tightly linked
molecular marker P1 was obtained. It is located at the physical position 26.45M on
chromosome 2 of rice, and its amplified fragment has the
base sequence of SEQ.ID.No.1 in the sequence listing. Studies have shown that the
molecular marker P1 of the present invention has a
population selection accuracy rate exceeding 96%, and can effectively and simply detect whether breeding rice materials contain
gene loci that confer resistance to rice leaf streak disease strains in breeding. Therefore, the present invention can be applied to rice disease resistance breeding, enabling simple, rapid, and accurate screening of rice leaf streak disease-resistant materials, thereby improving breeding efficiency.