The invention belongs to the field of molecular genetics, and discloses a molecular marker method for anti-rice blast gene Pi-hk1 (t) of paddy. The method comprises the following steps of: (1) selecting a paddy sample, and extracting genome deoxyribonucleic acid (DNA) of the paddy sample; and (2) performing polymerase chain reaction (PCR) amplification on the genome DNA of the paddy sample by utilizing any one, two or three pairs of molecular marker primers in s01, in5 and in3, performing electrophoretic detection on a PCR amplification product, and if corresponding DNA fragments of molecularmarkers are amplified, indicating that the Pi-hk1 (t) gene exists. In the method, the selection efficiency of a single marker is over 99.92 percent, and the selection efficiency of any double markersis 100 percent. The resistance level of the gene on rice blast can be forecasted by detecting whether the paddy contains the gene or not by the molecular marker primers provided by the invention, so the selection efficiency of the rice blast resistance of the paddy is improved greatly, and the process for breeding for disease resistance is accelerated.