384 results about "Molecular genetics" patented technology

The invention relates to the technical field of molecular genetic breeding, in particular to a KASP molecular marker related to salt tolerance in a mung bean germination period, a primer group, a kit and application. According to the KASP molecular marker Chr126769484 related to the salt tolerance in the mung bean germination period, primers are designed according to the KASP molecular marker, typing is carried out by utilizing specific matching of basic groups at the tail ends of the primers, the KASP molecular marker related to the salt tolerance in the mung bean germination period is developed, genetic typing is carried out, identification is accurate and convenient, the salt tolerance in the mung bean germination period can be predicted at low cost, and the application prospect is wide. The process of salt-tolerant variety breeding in the mung bean germination period is simplified, the breeding efficiency is improved, and the mung bean breeding process is accelerated.

The invention discloses an STON2 gene intron SNP (Single Nucleotide Polymorphism) molecular marker related to egg laying traits of chickens and application of the STON2 gene intron SNP molecular marker, and belongs to the technical field of molecular genetics. The research finds that the STON2 gene may participate in the related process of poultry sexual precocity and egg laying, so that the reproductive performance and the egg laying character of the chicken are influenced, and four SNP loci remarkably related to the egg laying character of the chicken are found in the fifth intron region and the sixth intron region of the STON2 gene for the first time. Based on the SNP site, the invention further develops an SNP molecular marker related to the egg laying traits of the chickens, and the SNP molecular marker can be used for auxiliary breeding of high-yield laying hens.

The invention belongs to the field of SNP (Single Nucleotide Polymorphism) detection, and particularly provides an SNP molecular marker related to a sheep body length character, application of the SNP molecular marker and a kit for detecting the SNP molecular marker. The kit provided by the invention is used for performing polymorphism detection on the SNP molecular genetic marker, namely the 92690768th site of the No.4 chromosome, screening and determining the sheep body length character, and selecting the body length character to breed or assist to breed a long sheep individual with the body length character, so that a Tibetan sheep variety germplasm resource with high-quality body length character can be collected, and the kit has a very good application prospect.

The invention discloses a molecular marker combination related to a cockscomb height character and a detection reagent and application thereof, and belongs to the technical field of molecular genetics. According to the molecular marker combination and the detection reagent thereof, ten SNP loci remarkably associated with the chicken first-laying crown height and the 130-day-old crown height are identified in the intron of the CTCF gene for the first time, based on the SNP loci, the molecular marker combination related to the cockscomb height character and the detection reagent thereof are designed, and the molecular marker combination and the detection reagent thereof can be used for early screening of laying hens with high cockscomb height and can be used for early screening of laying hens with high cockscomb height. The method is of great significance to breeding and breeding of precocious high-quality laying hens.

The invention discloses an Indel marker related to a corn kernel protein content character and application of the Indel marker, and belongs to the technical field of plant molecular genetics and crop molecular design breeding. The Indel marker is located on a gdh2 gene of a chromosome 10 of a corn genome, and insertion or deletion of a nucleotide sequence as shown in SEQ ID NO.5 occurs in an intron region between a second exon and a third exon. By applying the molecular marker-assisted selection technology, high-protein new germplasms S3317 (the crude protein content is 17.0%) and JS231148 (the crude protein content is 15.6%) have been successfully cultivated, the increase amplitudes of the high-protein new germplasms and JS231148 are both more than 15% compared with those of the conventional control, and the lysine content reaches 0.41% and reaches the national high-quality protein corn standard.

The invention provides a DGAT1 gene SNPs marker for detecting sheep milk production traits and application thereof.Nucleotide sequence variation sites of the DGAT1 gene are detected with the first-filial generation of Dongfudrisheng sheep male * Hu sheep female as an object, a general linear mixing effect model is constructed, the correlation between nucleotide sequence variation and sheep milk production traits is researched, and the DGAT1 gene SNPs marker for detecting the sheep milk production traits is obtained. And digging a molecular genetic marker for regulating and controlling the lactation character of the sheep. Three SNPs (single nucleotide polymorphisms) sites are found in total; wherein two variation sites in the first intron are named as c.191 + 411 Cgt, and the second intron is named as c.191 + 411 Cgt; t (SNP1) and c.192-440 Cgt, and c. 292-440 Cgt; t (SNP2) and a variation site in the 17th exon are named as c.1461 Cgt; t (SNP3). For the SNP1 site, the average daily milk yield of the CT genotype ewe is increased by 8.2% and 17.04% compared with that of the CC genotype ewe and that of the TT genotype ewe respectively; for the SNP3 site, the milk fat percentage of the CC genotype ewe is improved by 0.314% compared with that of the CT genotype ewe. A new molecular marker can be provided for selection and improvement of lactation traits of the milk sheep, and the molecular marker has important application prospects in sheep screening or breeding.

The invention discloses an SNP (Single Nucleotide Polymorphism) marker related to a soybean branching number gene QNE1 and application thereof, and relates to the technical field of molecular genetics and crop genetic breeding, the SNP molecular marker is a nucleotide sequence as shown in SEQ ID NO.1 formed by single nucleotide mutation at the 686th site of the nucleotide sequence of the QNE1 gene in a soybean genome; the polymorphism of the single nucleotide mutation is C / G polymorphism. The method is used for assisting soybean breeding. The SNP marker provided by the invention can be used for molecular marker-assisted selection of soybean branch number related traits, and a key tool is provided for cultivating soybean varieties adapting to different planting requirements (such as a few-branch close planting type or a multi-branch high-yield type).

The invention discloses application of an SNAC1 gene in improving resistance of rice to rice blast. The nucleotide sequence of the SNAC1 gene is as shown in SEQ ID NO. 1, and the amino acid sequence coded by the SNAC1 gene is as shown in SEQ ID NO. 2. And the SNAC1 gene is used for editing a strain. The resistance of the rice to the rice blast can be obviously improved. The invention provides a basis for the key molecular genetic mechanism of rice blast resistance, and provides important guidance for cultivating resistant rice varieties and improving rice quality.

The invention belongs to the field of molecular genetics, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker combination for genetic relationship identification of wuzhu cattle and application of the SNP molecular marker combination. The SNP marker combination is composed of 140 high-polymorphism SNP sites distributed on a plurality of chromosomes of a bovine whole genome, the average distance between the sites is reasonable, and the SNP marker combination has good genome coverage. The selected SNP site has high allele frequency (MAF), expected heterozygosity (He) and polymorphic information content (PIC), and the cumulative exclusion probability is 0.9999. King and Plink software are combined to deduce a genetic relationship coefficient (Kinship) and an IBD shared value (PIHAT) between samples, and a result shows that the marker combination can realize a genetic relationship recognition effect equivalent to that of whole genome SNP data in Wuzhu white cattle (grassland white cattle), and the scientificity and practicability of the marker combination are verified. The SNP molecular marker combination can be widely applied to the processes of germplasm resource management, pedigree correction and cattle breeding, and has good popularization prospects and economic benefits.

The invention relates to a pathogenic gene identification method and system based on multi-agent debate and a medium. The method comprises the following steps: acquiring gene detection data and clinical phenotype data; the data agent processes the acquired data, and calls a gene variation pathogenicity analysis operator and a molecular genetic large model to obtain first pathogenic gene information; the knowledge agent calls a molecular genetic large model to obtain second pathogenic gene information based on the first pathogenic gene information; the knowledge and data agents sequentially speak and debate pathogenicity of candidate pathogenic genes in the first or second pathogenic gene information on the basis of sorting results in the pathogenic gene information output by the knowledge and data agents; after each round of debate is finished, the debate agent judges whether the sorting results of the data and the knowledge agent on the candidate pathogenic genes are consistent or not, if the sorting results are consistent or the number of debate rounds reaches a threshold value, debate is finished, the debate agent conducts reasoning and outputs a result, and if not, the next round of debate is started. Compared with the prior art, the method has the advantages of no dependence on large-scale training data, high interpretability and the like.

The invention relates to the technical field of animal molecular genetics, molecular marker breeding and aquatic genetic breeding, in particular to a Koi-TAT gene related to golden and silver scale traits of fancy carp, an SNP molecular marker, a primer pair and application. According to the invention, a functional gene highly associated with the Koi gold and silver scale character is found, a specific molecular marker and a detection method are developed on the basis of the gene, and by utilizing the molecular marker and the detection method disclosed by the invention, an individual with excellent gold and silver scale character can be efficiently identified, and the molecular marker is suitable for molecular-assisted selective breeding and early-stage seed selection. The invention provides an important molecular tool for phenotype genetic improvement of aquarium fish, also provides a basis for molecular mechanism research of gold and silver scale formation, and has a wide application prospect.

The invention provides a KASP molecular marker related to mesosulfuron resistance of wheat and application of the KASP molecular marker, and belongs to the technical field of crop molecular genetic breeding. Through correlation analysis, it is found that the KASP molecular marker is remarkably correlated with mesosulfuron-methyl resistance, T / C polymorphism exists, and the mesosulfuron-methyl resistance of CC homozygous wheat is remarkably higher than that of TT homozygous wheat. On the basis, a KASP labeling reagent or kit containing a specific primer group is developed, the primer group comprises two allele specific upstream primers and a universal downstream primer, and the 5'ends of the upstream primers are connected with FAM and HEX fluorescent labels respectively. By optimizing a PCR reaction system and a landing procedure, high-throughput genotyping can be realized. The mesosulfuron-methyl-resistant wheat variety can be rapidly screened by applying the KASP molecular marker, a technical support is provided for herbicide resistance molecular breeding, the breeding efficiency of the resistant variety is remarkably improved, and the KASP molecular marker has important significance for guaranteeing safe production of wheat.

The invention belongs to the technical field of soybean molecular genetic breeding, and discloses a KASP marker for detecting an SNP site related to a soybean petiole included angle and application of the KASP marker. The SNP site is located at the 4589285th site on a chromosome 10 of a soybean reference genome Glycine max Wm82. A4. V1, and the site is subjected to C / T basic group replacement. Three KASP primers as shown in SEQ ID NO.2-4 are developed according to the SNP site, PCR amplification and genetic typing are performed on to-be-detected soybeans by using the primer group, and if a detection result shows that the genetic typing of the marker position is 1 / 1, it is judged that the petiole angle of the soybean variety is large; and if the detection result is 0 / 0, judging that the petiole angle is small. The SNP molecular marker can be used for molecular marker-assisted selection of soybean petiole included angle characters, and has important theoretical and practical guiding significance for accelerating genetic improvement of soybean compact plant types and improving breeding selection efficiency. The marker can rapidly identify the soybean petiole included angle, the method is simple and rapid, the identification result is accurate, and the marker has good popularization and application prospects.

The invention discloses a plant gene function analysis method (FAGEN), and belongs to the technical field of molecular genetics and functional genomics. The method comprises the following steps: constructing a target gene mutant by using a gene editing technology; hybridizing the mutant and the corresponding wild type as a common parent with a plurality of parents with different genetic backgrounds to construct a pair F1 group; an F2 group is obtained through selfing; and determining phenotypic data of target characters of F1 and F2 populations, and comparing phenotypic differences of filial generations of wild types and mutants under different genetic backgrounds to judge the functions of the target genes. The method has the advantages of simplicity in operation, flexibility in design, short construction period and low cost, and gene functions can be efficiently and accurately analyzed under various genetic backgrounds.

The invention discloses a molecular marker closely linked with peanut yield related traits and application, and belongs to the technical field of plant molecular genetics and molecular breeding. The molecular marker disclosed by the invention is Arahy0036; the nucleotide sequence of Arahy0036 in peanuts with low single plant yield is shown as SEQ ID NO: 1, and the nucleotide sequence of Arahy0036 in peanuts with high single plant yield is shown as SEQ ID NO: 2. The marker is used for detecting the gene locus related to the yield character of the peanut single plant, the single plant with high yield can be identified after seeds are harvested, other single plants are selected, reserved and eliminated, the breeding scale can be effectively controlled, and the progress and efficiency of variety selection breeding are improved.

The invention is suitable for the technical field of molecular genetics, and provides an SNP molecular marker related to pig backfat thickness and application thereof. The invention studies and determines that the pig backfat thickness related molecular marker is located at the 95583333rd site (corresponding to the 701st basic group A / G mutation of SEQ ID NO: 1) of the No.18 chromosome of the international pig reference genome version 11.1, and verifies the influence effect of the molecular marker on the pig backfat thickness character. And when the basic group at the site is A, the pig has lower back fat thickness. On the basis, a molecular marker-assisted selection breeding technology for rapidly improving the backfat thickness character of the pig is established, the frequency can be increased generation by generation by optimizing the dominant allele (A), the backfat thickness of the breeding pig is remarkably reduced, the genetic improvement process of commercial pig herds containing landrace and large white pig blood lineage is accelerated, and the breeding efficiency is improved; the sales profit is increased for enterprises, the core competitiveness is improved, and the economic benefits of commercial pigs are improved.

The invention relates to the technical field of livestock molecular genetic breeding, in particular to application of a molecular marker for grassland short-tailed sheep body weight character breeding, the molecular marker is a nucleotide sequence as shown in SEQ ID NO.1, and nucleotide at the 101bp site is G or A; the application refers to any one of the following steps: identifying the weight of the grassland short-tailed sheep; the body weight of the grassland short-tailed sheep offspring is improved. The SNP molecular marker obviously related to the body weight character is identified on the 24 # chromosome of the grassland short-tailed sheep for the first time. The molecular marker can be used for genotype detection in a young sheep stage or an embryo stage, and does not need to wait for stable phenotype when the sheep grow to an adult stage, so that the breeding cycle is shortened, and the genetic progress is remarkably accelerated. The technical blank in the field of grassland short-tailed sheep body weight character molecular breeding is filled, and a reliable molecular tool and technical support are provided for efficient, accurate and sustainable breeding of the breed.

The application discloses PRR30 gene SNP and a haplotype combination thereof in identifying bull semen quality, and belongs to the technical field of molecular genetics. Eight SNP sites are identified, wherein SNP7: g.794 C>A or SNP8: g.2247 G>T can be used for identifying the high and low of bull semen collection amount, SNP2: g.-2151 C>T can be used for identifying the high and low of bull sperm abnormality rate, and the haplotype combination of SNP1-SNP8 is that the bull with H1H11 has higher semen collection amount and lower sperm abnormality rate, and can be further used for identifying bull semen quality, thereby helping staff to evaluate bull breeding performance in an early stage, to carry out superior gene bull screening, to promote the cultivation of superior bull, to shorten the screening period and to improve the breeding efficiency.

The invention relates to the technical field of biology, in particular to a ZmTCP19 gene mutant and application of the ZmTCP19 gene mutant to regulation and control of the nitrogen utilization rate, the growth amount and the plant height of corn. ZmTCP19 gene mutants (a mutant zmtcp19-1 and a mutant zmtcp19-2) are obtained through a molecular genetics means, the functions of the ZmTCP19 gene mutants are deeply analyzed and identified, and results show that under the condition of low nitrogen, the total fresh weight of the zmtcp19-1 is reduced by 30.33% compared with that of a control group, and under the condition of normal nitrogen, the total fresh weight of the zmtcp19-1 is reduced by 25.54% compared with that of the control group; under the condition of low nitrogen, the total fresh weight of zmtcp19-2 is reduced by 25.19% compared with that of a control group, and under the condition of normal nitrogen, the total fresh weight is reduced by 17.30% compared with that of the control group. Meanwhile, the ZmTCP19 gene mutant can reduce the plant height of the corn and improve the lodging resistance of the corn.

This invention belongs to the field of molecular genetics and discloses a method and application for controlling fish fertility using in vivo gene editing technology. The applicant, for the first time, cloned a carp-specific U6 promoter and used this promoter to construct the U6 gene. MOVIE gRNA transgenic vectors and water The Cas9 vector allows for the establishment of an in vivo editing system. This strategy enables the heritability of sterility, a reproductive control trait, through hybridization of fertile parents to produce sterile offspring. The operation is highly efficient, simple, and environmentally friendly. Furthermore, since high-copy-rate short, scattered repetitive sequences exist in all fish species, this strategy has broad applicability across species.

The present application belongs to the field of molecular genetics, and particularly relates to an ethylene response transcription factor HvERF62 for regulating the moisture tolerance of barley and application thereof in molecular marker assisted selection breeding. A site significantly affecting the moisture tolerance of barley is identified through whole genome association analysis, and a candidate gene is predicted, and further, the moisture tolerance function of the candidate gene HvERF62 is verified by using the CRISPR-Cas9 gene editing method. The function of HvERF62 for regulating the moisture tolerance of barley is determined by using the gene editing technology, and an InDel molecular marker for detecting the moisture tolerance of barley is developed. The molecular marker of the present application is detected by using 3% agarose gel, is stable, simple in method, and can be used for screening the moisture tolerance of barley at an early stage, and has a strong application value in the breeding of the moisture tolerance of barley.

The present invention relates to a method for increasing corn yield, belonging to the field of molecular genetics, and discloses a corn yield regulating gene and a method for increasing corn yield and / or biomass.

The present invention is directed to fluorescent rhodamine dyes having spectral properties suited to the creation of multiplex assay systems for use in molecular biology, cell biology and molecular genetics. The rhodamine dyes have the following structure.

The invention relates to the technical field of plant molecular genetic breeding, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker closely linked with white gourd pulp malic acid major QTL (Quantitative Trait Loci) and application thereof. The nucleotide sequence of the SNP molecular marker is as shown in SEQ ID NO.4, and G / A mutation exists at the 26th basic group of the sequence. The molecular marker and the white gourd pulp malic acid have a close linkage marker characteristic, so that a KSAP primer pair is developed on the basis of the SNP molecular marker. Results of the specific embodiment of the invention show that the KASP molecular marker provided by the invention can be used for identifying the content of malic acid in pulp of wax gourd seeds or early-stage seedlings with true leaves, and the method is high in accuracy, low in cost and short in consumed time. Therefore, the SNP molecular marker and the KASP primer pair provided by the invention can be used for molecular marker-assisted selective breeding of white gourds, and the process of white gourd flavor, taste and quality breeding is accelerated.

This invention provides a gene related to the glossy leaf trait in Rugao black cabbage and its application, belonging to the field of molecular genetics and breeding technology. Specifically, this invention identifies the gene controlling the glossy leaf trait in 'Rugao black cabbage', confirms that this trait is controlled by a single dominant gene, and successfully locates the candidate gene. BraA02g02617P This study confirmed the association between the gene mutation and the glossy leaf trait. The discovery of the new dominant gene controlling glossy leaves greatly improves the convenience of molecular breeding of cruciferous plants, especially Brassica species, and has promising application prospects.

The invention discloses a chicken FMNL1 gene promoter region molecular marker, a detection reagent and application thereof, and belongs to the technical field of molecular genetics. According to the invention, two SNP loci significantly associated with egg laying performance are identified in a chicken FMNL1 gene promoter region, and the two SNP loci generally exist in a plurality of different varieties of chickens and present a consistent effect trend between different genotypes. Based on the SNP locus identified by the invention, the invention develops and designs a chicken FMNL1 gene promoter region molecular marker and a corresponding detection reagent. The method can be used for accurately identifying the laying day age and egg laying traits of the chickens in the early stage, and has important significance on breeding of high-yield laying hens.

The invention belongs to the technical field of molecular genetics, and relates to a stichopus japonicus whole life history on-site rapid sex determination molecular marker, a kit and application. The nucleotide sequence of the molecular marker is shown as SEQ ID NO.1 in a sequence table. The stichopus japonicus with the molecular marker is male. The invention also provides a primer for amplifying the molecular marker, a loop-mediated isothermal amplification detection primer group, a kit, an identification method and the like. The molecular marker and the developed kit can be used for rapid sex identification of the stichopus japonicus in the whole life history, and the problem of accurate sex identification in stichopus japonicus culture is solved.

This invention relates to the field of molecular genetics, specifically to a SNP molecular marker closely linked to a major QTL related to bolting in spinach and its application. The SNP molecular marker provided by this invention is KMBL29, with its polymorphic site located at position 47693313 on spinach chromosome 1, exhibiting an A / G polymorphism. A genotype of AA corresponds to bolting resistance in spinach; genotypes of AG or GG correspond to bolting susceptibility. This invention also provides specific primers based on KASP technology for genotyping this SNP molecular marker. This molecular marker and primers can be used to breed high-quality spinach varieties resistant to bolting, shortening the breeding cycle.

The invention discloses a molecular marker closely linked with peanut pod size character, a primer pair and application, and belongs to the technical field of plant molecular genetics and molecular breeding. The molecular marker disclosed by the invention is Arahy0055; the nucleotide sequence of the Arahy0055 in the peanuts with small pods is as shown in SEQ ID NO: 1, and the nucleotide sequence of the Arahy0055 in the peanuts with large pods is as shown in SEQ ID NO: 2. The marker is used for detecting gene loci related to the size character of the peanut pods, single plants of the large pods can be identified after seeds are harvested, other single plants are selected, reserved and eliminated, the breeding scale can be effectively controlled, and the progress and efficiency of variety selection breeding can be improved.

The invention discloses a KASP molecular marker related to the oil content of peanuts and application, and belongs to the field of molecular genetic breeding, an SNP site is positioned in a peanut A03 chromosome and named as qOCA03.1, the size of a positioning interval is in a 102kb range between A03: 8591523-8693979, the SNP site is positioned at 8641246bp of the A03 chromosome, and the polymorphism is T / C. The primer sequences of the molecular marker are respectively as follows: a universal forward primer F, the nucleotide sequence of which is as shown in SEQ ID NO.1: 5 '-TTGAGCGAATACCCCATCCG-3', a universal reverse primer F, the nucleotide sequence of which is as shown in SEQ ID NO.2, a universal reverse primer F, the nucleotide sequence of which is as The nucleotide sequence of the reverse primer R1 is as shown in SEQ ID NO.2: 5 '-CGTTGCCCTTTCGAGATAATTGTT-3'; the nucleotide sequence of the reverse primer R1 is as shown in SEQ ID NO.2; and the nucleotide sequence of the reverse primer R2 is as shown in SEQ ID NO.3: 5 '-CGTTGCCCTTTCGAGATAATTGTC-3'. The invention also discloses a kit for detecting the disease resistance of the human body, and a kit for detecting the disease resistance of the human body. According to the invention, through whole genome association analysis of oil content traits, significant SNP loci related to peanut oil content are identified, and KASP primers are designed and KASP markers are developed according to genetic variation information of positioned candidate intervals and candidate genes. Based on the KASP typing technology, the genotype of the peanut sample to be detected is rapidly and accurately identified, and the breeding efficiency of the peanut variety is improved.