219 results about "Molecular genetics" patented technology

The invention discloses a molecular marker combination related to a cockscomb height character and a detection reagent and application thereof, and belongs to the technical field of molecular genetics. According to the molecular marker combination and the detection reagent thereof, ten SNP loci remarkably associated with the chicken first-laying crown height and the 130-day-old crown height are identified in the intron of the CTCF gene for the first time, based on the SNP loci, the molecular marker combination related to the cockscomb height character and the detection reagent thereof are designed, and the molecular marker combination and the detection reagent thereof can be used for early screening of laying hens with high cockscomb height and can be used for early screening of laying hens with high cockscomb height. The method is of great significance to breeding and breeding of precocious high-quality laying hens.

The invention discloses an Indel marker related to a corn kernel protein content character and application of the Indel marker, and belongs to the technical field of plant molecular genetics and crop molecular design breeding. The Indel marker is located on a gdh2 gene of a chromosome 10 of a corn genome, and insertion or deletion of a nucleotide sequence as shown in SEQ ID NO.5 occurs in an intron region between a second exon and a third exon. By applying the molecular marker-assisted selection technology, high-protein new germplasms S3317 (the crude protein content is 17.0%) and JS231148 (the crude protein content is 15.6%) have been successfully cultivated, the increase amplitudes of the high-protein new germplasms and JS231148 are both more than 15% compared with those of the conventional control, and the lysine content reaches 0.41% and reaches the national high-quality protein corn standard.

The invention discloses an SNP (Single Nucleotide Polymorphism) marker related to a soybean branching number gene QNE1 and application thereof, and relates to the technical field of molecular genetics and crop genetic breeding, the SNP molecular marker is a nucleotide sequence as shown in SEQ ID NO.1 formed by single nucleotide mutation at the 686th site of the nucleotide sequence of the QNE1 gene in a soybean genome; the polymorphism of the single nucleotide mutation is C / G polymorphism. The method is used for assisting soybean breeding. The SNP marker provided by the invention can be used for molecular marker-assisted selection of soybean branch number related traits, and a key tool is provided for cultivating soybean varieties adapting to different planting requirements (such as a few-branch close planting type or a multi-branch high-yield type).

The invention discloses application of an SNAC1 gene in improving resistance of rice to rice blast. The nucleotide sequence of the SNAC1 gene is as shown in SEQ ID NO. 1, and the amino acid sequence coded by the SNAC1 gene is as shown in SEQ ID NO. 2. And the SNAC1 gene is used for editing a strain. The resistance of the rice to the rice blast can be obviously improved. The invention provides a basis for the key molecular genetic mechanism of rice blast resistance, and provides important guidance for cultivating resistant rice varieties and improving rice quality.

The invention relates to the technical field of animal molecular genetics, molecular marker breeding and aquatic genetic breeding, in particular to a Koi-TAT gene related to golden and silver scale traits of fancy carp, an SNP molecular marker, a primer pair and application. According to the invention, a functional gene highly associated with the Koi gold and silver scale character is found, a specific molecular marker and a detection method are developed on the basis of the gene, and by utilizing the molecular marker and the detection method disclosed by the invention, an individual with excellent gold and silver scale character can be efficiently identified, and the molecular marker is suitable for molecular-assisted selective breeding and early-stage seed selection. The invention provides an important molecular tool for phenotype genetic improvement of aquarium fish, also provides a basis for molecular mechanism research of gold and silver scale formation, and has a wide application prospect.

The invention provides a KASP molecular marker related to mesosulfuron resistance of wheat and application of the KASP molecular marker, and belongs to the technical field of crop molecular genetic breeding. Through correlation analysis, it is found that the KASP molecular marker is remarkably correlated with mesosulfuron-methyl resistance, T / C polymorphism exists, and the mesosulfuron-methyl resistance of CC homozygous wheat is remarkably higher than that of TT homozygous wheat. On the basis, a KASP labeling reagent or kit containing a specific primer group is developed, the primer group comprises two allele specific upstream primers and a universal downstream primer, and the 5'ends of the upstream primers are connected with FAM and HEX fluorescent labels respectively. By optimizing a PCR reaction system and a landing procedure, high-throughput genotyping can be realized. The mesosulfuron-methyl-resistant wheat variety can be rapidly screened by applying the KASP molecular marker, a technical support is provided for herbicide resistance molecular breeding, the breeding efficiency of the resistant variety is remarkably improved, and the KASP molecular marker has important significance for guaranteeing safe production of wheat.

The invention belongs to the technical field of soybean molecular genetic breeding, and discloses a KASP marker for detecting an SNP site related to a soybean petiole included angle and application of the KASP marker. The SNP site is located at the 4589285th site on a chromosome 10 of a soybean reference genome Glycine max Wm82. A4. V1, and the site is subjected to C / T basic group replacement. Three KASP primers as shown in SEQ ID NO.2-4 are developed according to the SNP site, PCR amplification and genetic typing are performed on to-be-detected soybeans by using the primer group, and if a detection result shows that the genetic typing of the marker position is 1 / 1, it is judged that the petiole angle of the soybean variety is large; and if the detection result is 0 / 0, judging that the petiole angle is small. The SNP molecular marker can be used for molecular marker-assisted selection of soybean petiole included angle characters, and has important theoretical and practical guiding significance for accelerating genetic improvement of soybean compact plant types and improving breeding selection efficiency. The marker can rapidly identify the soybean petiole included angle, the method is simple and rapid, the identification result is accurate, and the marker has good popularization and application prospects.

The invention discloses a plant gene function analysis method (FAGEN), and belongs to the technical field of molecular genetics and functional genomics. The method comprises the following steps: constructing a target gene mutant by using a gene editing technology; hybridizing the mutant and the corresponding wild type as a common parent with a plurality of parents with different genetic backgrounds to construct a pair F1 group; an F2 group is obtained through selfing; and determining phenotypic data of target characters of F1 and F2 populations, and comparing phenotypic differences of filial generations of wild types and mutants under different genetic backgrounds to judge the functions of the target genes. The method has the advantages of simplicity in operation, flexibility in design, short construction period and low cost, and gene functions can be efficiently and accurately analyzed under various genetic backgrounds.

The invention relates to the technical field of livestock molecular genetic breeding, in particular to application of a molecular marker for grassland short-tailed sheep body weight character breeding, the molecular marker is a nucleotide sequence as shown in SEQ ID NO.1, and nucleotide at the 101bp site is G or A; the application refers to any one of the following steps: identifying the weight of the grassland short-tailed sheep; the body weight of the grassland short-tailed sheep offspring is improved. The SNP molecular marker obviously related to the body weight character is identified on the 24 # chromosome of the grassland short-tailed sheep for the first time. The molecular marker can be used for genotype detection in a young sheep stage or an embryo stage, and does not need to wait for stable phenotype when the sheep grow to an adult stage, so that the breeding cycle is shortened, and the genetic progress is remarkably accelerated. The technical blank in the field of grassland short-tailed sheep body weight character molecular breeding is filled, and a reliable molecular tool and technical support are provided for efficient, accurate and sustainable breeding of the breed.

The application discloses PRR30 gene SNP and a haplotype combination thereof in identifying bull semen quality, and belongs to the technical field of molecular genetics. Eight SNP sites are identified, wherein SNP7: g.794 C>A or SNP8: g.2247 G>T can be used for identifying the high and low of bull semen collection amount, SNP2: g.-2151 C>T can be used for identifying the high and low of bull sperm abnormality rate, and the haplotype combination of SNP1-SNP8 is that the bull with H1H11 has higher semen collection amount and lower sperm abnormality rate, and can be further used for identifying bull semen quality, thereby helping staff to evaluate bull breeding performance in an early stage, to carry out superior gene bull screening, to promote the cultivation of superior bull, to shorten the screening period and to improve the breeding efficiency.

This invention belongs to the field of molecular genetics and discloses a method and application for controlling fish fertility using in vivo gene editing technology. The applicant, for the first time, cloned a carp-specific U6 promoter and used this promoter to construct the U6 gene. MOVIE gRNA transgenic vectors and water The Cas9 vector allows for the establishment of an in vivo editing system. This strategy enables the heritability of sterility, a reproductive control trait, through hybridization of fertile parents to produce sterile offspring. The operation is highly efficient, simple, and environmentally friendly. Furthermore, since high-copy-rate short, scattered repetitive sequences exist in all fish species, this strategy has broad applicability across species.

The invention relates to the technical field of plant molecular genetic breeding, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker closely linked with white gourd pulp malic acid major QTL (Quantitative Trait Loci) and application thereof. The nucleotide sequence of the SNP molecular marker is as shown in SEQ ID NO.4, and G / A mutation exists at the 26th basic group of the sequence. The molecular marker and the white gourd pulp malic acid have a close linkage marker characteristic, so that a KSAP primer pair is developed on the basis of the SNP molecular marker. Results of the specific embodiment of the invention show that the KASP molecular marker provided by the invention can be used for identifying the content of malic acid in pulp of wax gourd seeds or early-stage seedlings with true leaves, and the method is high in accuracy, low in cost and short in consumed time. Therefore, the SNP molecular marker and the KASP primer pair provided by the invention can be used for molecular marker-assisted selective breeding of white gourds, and the process of white gourd flavor, taste and quality breeding is accelerated.

This invention provides a gene related to the glossy leaf trait in Rugao black cabbage and its application, belonging to the field of molecular genetics and breeding technology. Specifically, this invention identifies the gene controlling the glossy leaf trait in 'Rugao black cabbage', confirms that this trait is controlled by a single dominant gene, and successfully locates the candidate gene. BraA02g02617P This study confirmed the association between the gene mutation and the glossy leaf trait. The discovery of the new dominant gene controlling glossy leaves greatly improves the convenience of molecular breeding of cruciferous plants, especially Brassica species, and has promising application prospects.

The invention discloses a chicken FMNL1 gene promoter region molecular marker, a detection reagent and application thereof, and belongs to the technical field of molecular genetics. According to the invention, two SNP loci significantly associated with egg laying performance are identified in a chicken FMNL1 gene promoter region, and the two SNP loci generally exist in a plurality of different varieties of chickens and present a consistent effect trend between different genotypes. Based on the SNP locus identified by the invention, the invention develops and designs a chicken FMNL1 gene promoter region molecular marker and a corresponding detection reagent. The method can be used for accurately identifying the laying day age and egg laying traits of the chickens in the early stage, and has important significance on breeding of high-yield laying hens.

The invention belongs to the technical field of molecular genetics, and relates to a stichopus japonicus whole life history on-site rapid sex determination molecular marker, a kit and application. The nucleotide sequence of the molecular marker is shown as SEQ ID NO.1 in a sequence table. The stichopus japonicus with the molecular marker is male. The invention also provides a primer for amplifying the molecular marker, a loop-mediated isothermal amplification detection primer group, a kit, an identification method and the like. The molecular marker and the developed kit can be used for rapid sex identification of the stichopus japonicus in the whole life history, and the problem of accurate sex identification in stichopus japonicus culture is solved.

The invention discloses a molecular marker closely linked with peanut pod size character, a primer pair and application, and belongs to the technical field of plant molecular genetics and molecular breeding. The molecular marker disclosed by the invention is Arahy0055; the nucleotide sequence of the Arahy0055 in the peanuts with small pods is as shown in SEQ ID NO: 1, and the nucleotide sequence of the Arahy0055 in the peanuts with large pods is as shown in SEQ ID NO: 2. The marker is used for detecting gene loci related to the size character of the peanut pods, single plants of the large pods can be identified after seeds are harvested, other single plants are selected, reserved and eliminated, the breeding scale can be effectively controlled, and the progress and efficiency of variety selection breeding can be improved.

The invention discloses a molecular marker related to correction day age of 100 kg weight of a pig and application of the molecular marker, and belongs to the technical field of molecular genetics. According to the invention, for the first time, two SNP loci obviously associated with the weight correction age of a pig reaching 100 kg are identified in a CDS region of a GREB1 gene, and the two SNP loci show complete linkage imbalance and form a conservative haplotype block. The two mutation sites are significantly related to the corrected day age (Plt; 0.001) of the 100 kg weight of the pig, and the corrected day age of the GA genotype is significantly shorter than the corrected day age of the GG genotype and the AA genotype. Detection of the molecular marker associated with the correction day age reaching 100 kg is beneficial to breeding of meat varieties / lines with high growth speed, and has important significance for breeding of pig varieties.

This invention provides SSR molecular markers associated with the yield trait of Corydalis yanhusuo, belonging to the field of molecular genetics. This invention utilizes 24 pairs of SSR markers to perform regression analysis on the genotype and yield trait of individual Corydalis yanhusuo individuals. Using the GLM program, association loci for the yield trait of Corydalis yanhusuo were detected. The SSR molecular markers CYC2-4 and CYC8-10 were found to be highly significantly associated, explaining 16.72% and 14.06% of the phenotypic variation, respectively. The primers provided by this invention for amplifying the aforementioned SSR molecular markers can be used to screen for high-yielding Corydalis yanhusuo germplasm, locate key genes controlling yield traits, and for marker-assisted breeding, which is of great significance for cultivating high-yielding Corydalis yanhusuo varieties.

The application discloses a SNP molecular marker combination of a FGF9 gene promoter region related to chicken egg laying traits and application thereof, and belongs to the technical field of molecular genetics. The application finds that multiple SNP sites exist in the FGF9 gene promoter region of a chicken through screening of SNPs between breeds, and for the first time, carries out correlation analysis on the SNPs genotypes of a Jining Hundred-day chicken population and egg laying traits, finds that the GA ‑2177 genotype individual corresponds to more maximum consecutive egg laying numbers; the GA ‑2289 genotype individual corresponds to an earlier age of first egg laying; and the GA ‑3906 genotype individual corresponds to more total egg laying numbers. Therefore, through detection of the three molecular markers related to the egg laying traits, not only is the method simple and fast, but also it is helpful to breed a high egg laying chicken breed, and provides favorable help for breeding work.

The invention belongs to the technical field of molecular genetics, and particularly relates to a wheat powdery mildew resistance main effect QTL, a KASP molecular marker closely linked with the wheat powdery mildew resistance main effect QTL and application of the wheat powdery mildew resistance main effect QTL. The invention provides a wheat powdery mildew resistance major QTL, the major QTL locus is located at the end part of a wheat 1A chromosome short arm and is located in a 4.4 cM genetic interval between flanking KASP markers Chr1A6275522K2 and Chr1A11524652K9, nine KASP molecules are developed, auxiliary selective breeding and screening of powdery mildew resistance wheat strains can be carried out, and a new available molecular marker is provided for screening of wheat powdery mildew resistance materials.

The application discloses a SNP marker related to a chicken age of first egg laying character and application thereof, and belongs to the technical field of molecular genetics and gene breeding. The SNP marker is located at the position of 3298230 bp of chicken chromosome 27, the reference genome version is GRCg7b, has G / A polymorphism, and the individual with the AA genotype at the position has the early age of first egg laying character compared with other genotypes. The SNP marker can be applied to the breeding of excellent breeding hens, can significantly shorten the age of first egg laying of a population, and has great economic application value.

The invention belongs to the field of molecular genetics, and particularly relates to application of a corn transcription factor bZIP83 in regulation of corn plant height characters. The invention provides a transcription factor bZIP83 for regulating and controlling the height of a corn plant and a sequence of an encoding protein of the transcription factor bZIP83, and discloses a method for reducing the height of the corn plant by mutating a bZIP83 gene by utilizing a genetic engineering means.

This invention belongs to the field of animal molecular genetics and breeding technology, specifically relating to SNP molecular marker combinations for identifying kinship in Sunite Mongolian cattle and their applications. The marker combination consists of 141 highly polymorphic SNP loci distributed on bovine autosomes 1-29, with an average physical distance of 19.93 Mb between loci, exhibiting good genome coverage and locus independence. The selected SNP loci show high minimum allele frequency (MAF), expected heterozygosity (He), and polymorphism information content (PIC) in the Sunite Mongolian cattle population, with average values ​​of 0.4843, 0.4990, and 0.3745, respectively, and a cumulative exclusion probability of no less than 0.9999 under unknown maternal genotype conditions. The SNP molecular marker combination and its application method of this invention can provide a reliable molecular tool for the protection of Sunite Mongolian cattle germplasm resources, the construction of conservation populations, the selection of breeding bulls and cows, and the development of the Sunite Mongolian beef cattle industry, offering advantages such as ease of operation, moderate cost, and high accuracy.

The application discloses a molecular genetic marker affecting pig sexual maturity and application thereof. The application takes RBP1 as a research object, and adopts molecular and cell biology methods to study the correlation between RBP1 and the initial estrus period. It is found that six SNP sites in the RBP1 promoter region are related to the initial estrus period. Further research shows that the promoter activity of the CC genotype of the g.80408109T>C site is significantly higher than that of the TT genotype, the promoter activity of the AA genotype of the g.80409632A>G site is significantly higher than that of the GG genotype, and it is confirmed that RBP1 can inhibit the ferroptosis of granulosa cells. It can be seen that the mutation in the RBP1 promoter region can affect the expression of RBP1, and RBP1 can accelerate the initial estrus start by inhibiting the ferroptosis of pig ovarian granulosa cells. The application has good application value for the research on ovarian follicle development and initial estrus start.

The invention belongs to the field of plant molecular genetics and crop breeding, and provides a rapid identification method and a creation method of an extremely early rice variety. According to the invention, a specific genotype combination causing the rice to present an extremely early-maturing phenotype is clear and verified for the first time, i.e., the three genes Ghd7, Ghd7.1 and Ghd8 are represented as function deletion alleles, and meanwhile, the Hd1 gene is represented as a functional alleles. Based on the specific combination, genotype identification is performed on a plurality of known extremely early rice varieties in northeast and southern China, and results are completely identical. Furthermore, a novel rice material with the genotype combination is created through a gene editing technology, and a typical strain with an extremely early heading stage is successfully obtained, so that the accuracy and reliability of the combination are verified on the molecular level. According to the invention, the problem of inaccurate prediction caused by gene interaction complexity in the prior art is solved, and a powerful molecular tool is provided for early-maturing breeding of rice.

The application provides a cold-resistant gene TrWRKY10a540, an expression vector, application and breeding method thereof, and belongs to the field of plant molecular genetics and genetic improvement. Specifically disclosed is a cold-resistant gene TrWRKY10a540 and an expression vector derived from Trifolium repens L. and application thereof in improving the cold resistance of plants, and the problem of insufficient research on low-temperature stress related genes of Trifolium repens in the prior art is solved. The application further discloses a breeding method for regulating the cold stress resistance of plants by the cold-resistant gene TrWRKY10a540, and research results show that the gene can significantly improve the cold resistance and antioxidant level of plants under low-temperature stress conditions, and has good functional effects. The application can be widely applied to plant molecular breeding, is particularly suitable for improving the low-temperature resistance of economic crops such as forage grasses, provides a new genetic resource for the breeding of cold-resistant plants, and has important significance for revealing the regulation mechanism of the cold resistance of plants.

The present application relates to a method for increasing grain weight, and belongs to the field of molecular genetics. Two maize kernel type regulating genes are disclosed, and a method for increasing maize grain weight and yield by gene editing is disclosed.

The invention belongs to the field of rice molecular genetics, and particularly discloses an MBKbase database-based dominant allelotype analysis method for stem strength genes, which comprises the following steps: firstly, analyzing different genotypes of rice stem strength genes SCM3 and OsRLCK191, and according to different genotype germplasm resources of the rice stem strength genes carried in MBKbase-Rice, constructing a dominant allelotype of the rice stem strength genes SCM3 and OsRLCK191; the method comprises the following steps: selecting rice materials with single-dominant allelic genes, double-dominant allelic genes and non-dominant allelic genes (reference genome type), determining the stem strength character of rice germplasm resources of different genotype combinations of the rice stem strength gene, and finally performing sequencing verification on different genotypes of the rice stem strength gene. Finally, clearly breeding a strategy that the stalks are thick and strong, the stalks are high in elasticity or the stalks are moderate in thickness and elasticity to improve the lodging resistance of the rice variety; therefore, low-cost and high-throughput genotype screening can be realized.

The invention relates to the technical field of molecular genetic breeding, in particular to a molecular marker primer group for identifying the early flowering character of brassica napus and application of the molecular marker primer group, the molecular marker primer group is a group of KASP primers and is used for detecting a single nucleotide polymorphism site in a brassica napus BnCRY2 gene coding region; the single nucleotide polymorphism site is located at the 1259th site of the nucleotide sequence as shown in SEQ ID NO.11, and the basic group of the site is T or C. The molecular marker primer group developed by the invention is high in detection efficiency and accurate in typing, can be used for rapid identification, genetic analysis and molecular marker-assisted breeding of a brassica napus early flowering material, and provides an effective technical support for cultivating a new variety of extremely early-maturing brassica napus.

The invention belongs to the field of molecular genetics, and provides a rice SS3a allele and application thereof in increasing the content of resistant starch in rice and reducing the glucose generation rate. The gene is deleted at the 2387th basic group G of the third exon of the rice SS3a gene and contains a gene sequence as shown in SEQ ID No. 1. The allele can significantly increase the amylose content in rice and change the amylopectin structure, so that the resistant starch content of rice is significantly increased, and the glucose generation rate of rice is reduced. Meanwhile, the allele can also significantly improve the gelatinization characteristic and the viscosity characteristic of the rice, and has small influence on the grain weight of the rice. The invention further relates to application of the new SS3a allele in rice breeding, the allele can be introduced through a cross breeding method, or the original SS3a gene is subjected to site-directed mutagenesis to form the allele, and the allele is used for cultivating a new high-resistance starch rice strain.