575 results about "Molecular genetics" patented technology

The invention belongs to the field of SNP (Single Nucleotide Polymorphism) detection, and particularly provides an SNP molecular genetic marker related to a sheep horn length character and a kit for detecting the SNP molecular marker. The kit provided by the invention is used for performing polymorphism detection on the SNP molecular genetic marker, namely the 30991957th site of the 10th chromosome, screening and determining the horn length character of the sheep, and selecting the horn length character to breed or assist in breeding horn-free sheep individuals, so that the collection of high-quality horn-free Tibetan sheep variety germplasm resources can be ensured, and the kit has a very excellent application prospect.

The invention discloses a soybean SNP molecular marker combination, a chip and application thereof, and relates to the field of soybean molecular genetic breeding. The SNP molecular marker combination developed by the invention is uniformly distributed on 20 chromosomes of a soybean whole genome, and the accuracy of related research application such as gene positioning can be improved. The SNP molecular marker combination provided by the invention is rich in information amount and has important value in variety identification, correlation analysis and molecular marker-assisted breeding. On the basis, detection products such as SNP typing chips, kits and the like which are high in capture rate, high in density and high in polymorphism and are uniformly distributed in a genome can be developed.

The invention provides a KASP molecular marker related to a corn ear height character under a drought condition and application of the KASP molecular marker, and belongs to the technical field of molecular genetics. The SNP marker site related to the ear height character under the drought condition and having polymorphism is obtained through whole genome association analysis, the SNP marker site refers to a corn B73 RefGenv4 genome, when nucleotide polymorphism T / C exists at the position 147243591 of a 10 # chromosome, the SNP marker site is a dominant character, and nucleotide sequences used for amplifying a KASP molecular marker primer group are shown as SEQ ID NO.1-3. Gene typing identification can be rapidly, simply and efficiently carried out by utilizing a KASP typing technology, enzyme digestion and electrophoresis are not needed, accurate identification and selection can be carried out in a seedling stage, a large amount of breeding workload can be reduced, the period of high-ear-position breeding selection can be shortened, and the method can be used for high-ear-position molecular marker-assisted breeding of corn.

A resistance gene TaCAT to FCR of wheat and an application thereof are provided, which relates to the field of biological genetic engineering technologies. The nucleotide sequence of a genome where the TaCAT gene is located, the nucleotide sequence of a cDNA, the nucleotide sequence of a CDS and a coded protein sequence are respectively as shown in SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 and SEQ ID NO: 4. An InDel marker is developed for identifying different allelotypes of the TaCAT gene. An application of the TaCAT gene for preparation of transgenic plant cells and plant breeding is provided. The molecular genetic basis of the resistance to FCR is disclosed, an important role is played in improving the resistance to FCR by utilizing a genetic engineering technology, and a new way is provided for breeding a new wheat variety with disease-resistance and safety.

The invention relates to the technical field of molecular genetic breeding, in particular to a KASP molecular marker related to salt tolerance in a mung bean germination period, a primer group, a kit and application. According to the KASP molecular marker Chr126769484 related to the salt tolerance in the mung bean germination period, primers are designed according to the KASP molecular marker, typing is carried out by utilizing specific matching of basic groups at the tail ends of the primers, the KASP molecular marker related to the salt tolerance in the mung bean germination period is developed, genetic typing is carried out, identification is accurate and convenient, the salt tolerance in the mung bean germination period can be predicted at low cost, and the application prospect is wide. The process of salt-tolerant variety breeding in the mung bean germination period is simplified, the breeding efficiency is improved, and the mung bean breeding process is accelerated.

The invention belongs to the field of SNP (Single Nucleotide Polymorphism) detection, and particularly provides an SNP molecular marker related to a sheep horn length character and a kit for detecting the SNP molecular marker. The kit provided by the invention is used for performing polymorphism detection on the SNP molecular genetic marker, namely the 30947874th site of the 10th chromosome, screening and determining the horn length character of the sheep, and selecting the horn length character to breed or assist in breeding horn-free sheep individuals, so that the collection of high-quality horn-free Tibetan sheep variety germplasm resources can be ensured, and the kit has a very excellent application prospect.

The invention belongs to the technical field of molecular genetic breeding, and particularly relates to a molecular marker Zm00001eb181850 gene related to corn leukoderma resistance and application thereof, the invention provides a molecular marker related to the corn leukoderma resistance, the gene sequence of the molecular marker is shown as SEQ ID NO: 1 and / or SEQ ID NO: 2, and the molecular marker Zm00001eb181850 gene is shown as SEQ ID NO: 1 and / or SEQ ID NO: 2. Researches prove that corn white spot resistance QTL qMWS4-1 and qMWS4-5 intervals and resistance SNP: 4-118983592 have co-localization, the corn white spot resistance QTL has a high phenotypic interpretation rate, the associated gene Zm00001eb181850 (SEQ ID NO: 2) is highly expressed in corn leaves, it is proved that the associated gene Zm00001eb181850 (SEQ ID NO: 2) is associated with the corn leaf spot, the corn white spot resistance is a quantitative character jointly regulated by multiple genes, and the corn leaf spot resistance QTL has a good application prospect. According to the technical scheme provided by the invention, a new method is provided for cultivating a new variety of leukoderma-resistant corn by utilizing a modern molecular breeding technology.

The invention discloses an STON2 gene intron SNP (Single Nucleotide Polymorphism) molecular marker related to egg laying traits of chickens and application of the STON2 gene intron SNP molecular marker, and belongs to the technical field of molecular genetics. The research finds that the STON2 gene may participate in the related process of poultry sexual precocity and egg laying, so that the reproductive performance and the egg laying character of the chicken are influenced, and four SNP loci remarkably related to the egg laying character of the chicken are found in the fifth intron region and the sixth intron region of the STON2 gene for the first time. Based on the SNP site, the invention further develops an SNP molecular marker related to the egg laying traits of the chickens, and the SNP molecular marker can be used for auxiliary breeding of high-yield laying hens.

The invention relates to the field of molecular genetics, in particular to a cynomolgus monkey specific SNP site combination, a liquid chip and application of the cynomolgus monkey specific SNP site combination and the liquid chip, 1508 cynomolgus monkey specific SNP sites disclosed and screened by the invention have high polymorphism, and rapid identification, paternity identification and lifelong tracking identification of cynomolgus monkey individuals can be realized. Liquid phase chip design is carried out on the sites, chip inspection is carried out on 383 cynomolgus monkey samples from three different groups, the result shows that the average site detection rate is 99.47%, and it is indicated that the liquid phase chip has excellent site capturing efficiency. According to the method, the genetic detection capability and accuracy of the cynomolgus monkeys can be greatly improved, the genetic detection cost can be remarkably reduced, and an important technical support is provided for improving the breeding management of the cynomolgus monkeys and optimizing the population structure.

The invention discloses a dCAPS molecular marker for identifying a wheat leaf rust resistance associated SNP site and application of the dCAPS molecular marker, and belongs to the technical field of wheat disease resistance breeding and molecular biology. The invention develops a dCAPS molecular marker of SNP (Single Nucleotide Polymorphism) linked with a leaf rust resistance genetic locus QLr.hebau-1BS in a wheat adult-plant stage by virtue of 277 parts of wheat materials which have genotype data and are introduced from Turkey and in combination with a Chinese spring reference sequence. By utilizing the molecular marker, different wheat can be accurately and quickly detected, early-stage molecular-assisted selection of leaf rust resistant germplasm materials in the adult-plant stage is realized, and the wheat disease-resistant breeding efficiency is improved. The method solves the problem of high difficulty in detecting the leaf rust resistance gene in the wheat adult-plant stage in the prior art, and is suitable for the field of molecular genetic breeding.

The invention belongs to the field of SNP (Single Nucleotide Polymorphism) detection, and particularly provides an SNP molecular marker related to a sheep body length character, application of the SNP molecular marker and a kit for detecting the SNP molecular marker. The kit provided by the invention is used for performing polymorphism detection on the SNP molecular genetic marker, namely the 92690768th site of the No.4 chromosome, screening and determining the sheep body length character, and selecting the body length character to breed or assist to breed a long sheep individual with the body length character, so that a Tibetan sheep variety germplasm resource with high-quality body length character can be collected, and the kit has a very good application prospect.

The invention relates to the technical field of crop molecular genetic markers, and discloses an InDel molecular marker of avena nuda and application thereof.The molecular marker is a group of 314bp deletion variation and 2bp insertion variation in the 610439484-610440026 interval of the 5C chromosome position of a reference genome of an avena nuda variety OT3098, the nucleotide sequence of the deletion variation is shown as SEQ ID NO.1, the nucleotide sequence of the insertion variation is shown as SEQ ID NO.2, and the nucleotide sequence of the insertion variation is shown as SEQ ID NO.3. The nucleotide sequence of the insertion variation is 5 '-AA-3'. The molecular marker provided by the invention can realize stable detection in agarose gel electrophoresis by combining a specific primer pair (SEQ ID NO.2-3) with an optimized touchdown PCR (Polymerase Chain Reaction) program, has the advantage of being not influenced by a tissue part and a growth period, and provides an efficient and reliable technical means for oat germplasm identification, seed purity detection and molecular breeding.

The invention discloses a method for screening and identifying a cucumber aphid-resistant gene as well as a screened gene and application thereof, and provides a brand-new method for screening and identifying the cucumber aphid-resistant gene, which is used for screening the cucumber aphid-resistant gene based on four omics technologies. The four types of omics comprise transcriptome, proteome, methylation group and metabolome before and after inoculation of aphids on cucumbers, and the cucumber aphid resistance regulation gene is screened through combined analysis, so that a new gene resource is provided for improving the aphid resistance of the cucumbers, and a new method and a new idea are provided for designing molecular genetic breeding. The CsPGD2 gene obtained through screening is an aphid resistance gene, the dynamic change of a metabolic substance 5-ribulose phosphate in a metabolome is consistent with that of a transcriptome gene CsPGD2, the aphid resistance function of the gene CsPGD2 is verified through VIGS, and the result proves that the gene CsPGD2 is an important regulation factor for the aphid resistance of cucumbers. The invention provides a new gene resource for improving the aphid resistance of cucumbers, and has a good application prospect.

The invention provides a functional molecular marker of a wheat leaf rust resistance gene Lr.ace-4A, a detection method and application of the functional molecular marker. The functional molecular marker comprises an upstream primer as shown in SEQ ID NO: 1 and a downstream primer as shown in SEQ ID NO: 2. The method can solve the problem that the Lr.ace-4A gene in wheat is difficult to rapidly detect in the prior art, and is suitable for the field of molecular genetic breeding.

The invention discloses an SSR (simple sequence repeat) molecular marker associated with fresh weight character of corydalis tuber bulbules as well as primers and application of the SSR molecular marker, and belongs to the technical field of molecular genetics. According to the SSR molecular marker associated with the fresh weight character of the corydalis tuber bulbous disclosed by the invention, the amplification primer sequences of the SSR molecular marker are shown as SEQ ID NO.1 and SEQ ID NO.2. The SSR molecular marker associated with the fresh weight character of the rhizoma corydalis coleobulb and the amplification primer thereof can be applied to rhizoma corydalis population structure analysis, rhizoma corydalis coleobulb fresh weight character associated marker analysis, rhizoma corydalis excellent germplasm breeding and preparation of a kit for screening rhizoma corydalis excellent germplasm with high coleobulb weight. The method can be directly used for excellent germplasm screening, gene localization and cloning and molecular marker-assisted breeding, has chromosome localization information, is beneficial to further fine localization and cloning of genes for controlling fresh weight characters of corpus balls, and has important significance in breeding of high-yield rhizoma corydalis.

The invention discloses an InDel molecular marker related to wheat yield traits and application of the InDel molecular marker, and belongs to the technical field of molecular genetics. The InDel molecular marker is a molecular marker InDel 509; the nucleotide sequence of the molecular marker InDel 509 is as shown in SEQ ID No. 1. A wheat TaPRR95-5A gene functional marker InDel 509 is used for carrying out PCR (Polymerase Chain Reaction) amplification, polyacrylamide gel electrophoresis detection is carried out, and a germplasm material carrying a Hap II type is screened out according to the size of a target band. The functional marker can provide an effective marker resource for wheat genetic improvement and molecular marker-assisted breeding.

The invention discloses a wheat stripe rust resistance regulation gene TaWAK2 as well as an encoding protein and application thereof, and belongs to the technical field of molecular biology. The wheat stripe rust resistance regulation gene TaWAK2 disclosed by the invention is as shown in a sequence table SEQ ID NO: 1, and the length of the wheat stripe rust resistance regulation gene TaWAK2 is 7042bp; the amino acid sequence of the protein coded by the gene is shown as SEQ ID NO: 4 in a sequence table, and the length of the amino acid sequence is 693. Through a VIGS silencing technology and gene sequencing analysis, it is proved that the gene TaWAK2 plays an important role in wheat stripe rust resistance regulation and control, and the wheat stripe rust resistance is negatively regulated and controlled. The gene TaWAK2 disclosed by the invention can be applied to wheat stripe rust resistance breeding; research on the gene is beneficial to revealing a molecular genetic basis of wheat stripe rust resistance, and an important gene target is provided for wheat breeding for disease resistance.

The invention discloses a KASP molecular marker related to brassica napus quality traits and application of the KASP molecular marker, and belongs to the technical field of molecular genetics. According to the KASP molecular marker related to the brassica napus quality traits, the primers of the KASP molecular marker and the application of the KASP molecular marker and the primers in brassica napus genetic typing or quality traits identification, results show that the KASP molecular marker can accurately identify genotypes, is high in sensitivity and specificity and effectively reduces the error rate. The invention also discloses a brassica napus genotyping / quality identification method, and the result shows that the material with the genotyping result of AA has the characteristics of low erucic acid content, low thioglycoside content, high oleic acid content, high linoleic acid content, low arachidonic acid content and low protein content; gG-type materials exhibit high erucic acid, high thioglycoside, low oleic acid, low linoleic acid, high arachidonic acid and high protein content. Individuals with excellent quality characters can be rapidly screened in early generations, and the long process of character identification after plant maturation in traditional breeding is avoided.

The invention belongs to the technical field of gene detection and the field of crop molecular genetic breeding, and particularly relates to a sweet potato gene copy number detection method and application thereof. Specifically, according to the technology, transgenic sweet potatoes or arabidopsis thaliana carrying target genes (exogenous genes such as HPT, 35S, IbMYB1-4 and the like which are cloned to expression vectors, namely external reference genes) are taken as control materials, the control materials and sweet potato cultivated species needing to be analyzed are mixed according to a preset mass ratio, the target genes HPT of the transgenic materials are taken as the external reference genes on the basis of detection of the qPCR technology, and the sweet potato cultivated species needing to be analyzed are obtained. Or IbTUA, IbActin and the like in the screened sweet potato cultivar genome are taken as reference genes, and the relative copy number of the target gene in the genome of a sample to be detected can be quickly obtained by calculating the relative copy number between the target gene to be detected and the reference gene, so that data reference is provided for subsequent genetic research.

The invention belongs to the field of molecular genetics, and particularly relates to a method for reducing the plant height of corn. The invention provides a method for reducing the corn plant height through precise point mutation.

The invention relates to the technical field of molecular genetics, and particularly discloses a Yr6 gene, protein, a recombinant vector and application of the Yr6 gene, the protein and the recombinant vector, the full-length sequence of the Yr6 gene is shown as SEQ ID NO.1, and the CDS sequence of the Yr6 gene is shown as SEQ ID NO.2. Yr6 is overexpressed in a transgenic mode to improve wheat stripe rust resistance. The Yr6 gene provided by the invention is beneficial to improving the resistance of wheat to stripe rust, and provides support for breeding of stripe-rust-resistant wheat.

The invention belongs to the field of molecular genetic breeding, and particularly relates to a KASP molecular marker method related to peanut aspergillus flavus seed kernel infection resistance and application of the KASP molecular marker method. Through whole genome association analysis, the SNP locus is located at the 133198521th bp position of the peanut B06 chromosome (the reference genome is peanut), the nucleotide sequence polymorphism is C / A (the antisense strand is G / T), and the SNP locus is named as qARIB06. Based on the SNP site, KASP molecular marker primer sequences (SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3) are designed and developed, and the KASP molecular marker primer sequences are used for rapidly and accurately identifying the genotype of a peanut sample.

The invention discloses an FABP3 gene SNPs marker for detecting sheep milk production traits and application of the FABP3 gene SNPs marker, and belongs to the technical field of biology. According to the invention, lactating sheep is taken as a research object, Sanger and other technologies are applied to detect a nucleotide sequence variation site of an FABP3 gene, a general linear mixing effect model is constructed, the correlation between nucleotide sequence variation and sheep lactation traits is researched, and a molecular genetic marker for regulating and controlling the sheep lactation traits is excavated; on the SNP1 site, the cream-fat percentages of AG and GG genotype individuals are respectively 0.99% and 1.01% higher than those of AA type individuals. On the site SNP2, the milk fat rates of TC and TT genotype individuals are 1.18% and 1.23% higher than those of CC type individuals respectively, and the daily lactation volumes of the TC and TT genotype individuals are 1.97% and 1.27% higher than those of the CC type individuals respectively.

The invention discloses an OsRNE gene and application of protein coded by the OsRNE gene in rice leaf color regulation and chloroplast development. The nucleotide sequence of the gene OsRNE is as shown in SEQ ID NO.1, and the amino acid sequence of the encoded protein of the gene OsRNE is as shown in SEQ ID NO.2. According to the invention, the OsRNE is knocked out by using a CRISPR / Cas9 gene editing technology, and a functional loss mutant is obtained. The mutation of the gene causes damaged development of rice chloroplast, influences chlorophyll synthesis, albinism of seedlings and death within the trefoil stage, so that the gene can be used as a marker character in the seedling stage to assist rice molecular breeding. The OsRNE gene is mainly expressed in leaves, protein coded by the OsRNE gene is positioned in chloroplast, and development of the chloroplast and normal growth of seedlings are further affected by affecting metabolism of ribosome RNA in the chloroplast. Therefore, the method can be applied to rice leaf color character molecular genetic breeding, and has important significance in further understanding a rice chloroplast development regulation mechanism and improving the rice yield.

A molecular marker significantly associated with vitamin E content in soybeans, a Kompetitive Allele-Specific polymerase chain reaction (KASP) primers combination and an application thereof are provided in the present disclosure, belonging to the field of molecular genetics and breeding. A nucleotide sequence of the molecular marker is shown in SEQ ID NO. 1, and there is an A / T mutation at the 21st base. The KASP primer set for detecting the molecular marker includes an upstream primer F1 with a nucleotide sequence as shown in SEQ ID NO. 2, an upstream primer F2 with a nucleotide sequence as shown in SEQ ID NO. 3 and a downstream primer r with a nucleotide sequence as shown in SEQ ID NO. 4. The KASP primers combination developed by the present disclosure accurately distinguish soybeans with high and low vitamin E content.

The present disclosure pertains to the biological field, specifically to a combination of 33 single nucleotide polymorphism (SNP) loci associated with the wool fiber diameter of fine wool sheep, determined using Ovis aries reference genome version 4.0. It also covers the use of reagents for detecting genotypes of these SNP loci in wool fiber diameter analysis or molecular marker-assisted selection of fine wool sheep. Additionally, it includes molecular probe combinations, gene chips, and kits based on the SNP loci. These tools facilitate early-stage wool fiber trait analysis, enabling individual selection, reducing generation intervals, accelerating breeding processes, and lowering costs. This disclosure provides crucial support for the identification, breed preservation, and molecular genetic breeding of fine wool sheep.

The invention relates to the technical field of crop molecular genetic markers, and discloses an Indel molecular marker of avena nuda and application of the molecular marker, the molecular marker is insertion variation of 551bp located at the 4D chromosome position 451669339-451669889 of a reference genome of an avena nuda variety Sanfensan, and the insertion sequence is as shown in SEQ ID NO. 1. The Indel molecular marker provided by the invention is completely related to the naked oat skin character, can be efficiently used for naked oat germplasm resource identification, variety purity detection and molecular marker-assisted breeding, remarkably improves the screening efficiency of naked oat excellent germplasm, and provides a brand new molecular detection technology for genetic improvement of the naked oat skin character. According to the invention, not only is a rapid and accurate technical standard established for naked oat variety identification, but also important molecular marker resources and application schemes are provided for oat quality improvement and germplasm innovation.

The invention discloses a molecular marker combination related to a cockscomb height character and a detection reagent and application thereof, and belongs to the technical field of molecular genetics. According to the molecular marker combination and the detection reagent thereof, ten SNP loci remarkably associated with the chicken first-laying crown height and the 130-day-old crown height are identified in the intron of the CTCF gene for the first time, based on the SNP loci, the molecular marker combination related to the cockscomb height character and the detection reagent thereof are designed, and the molecular marker combination and the detection reagent thereof can be used for early screening of laying hens with high cockscomb height and can be used for early screening of laying hens with high cockscomb height. The method is of great significance to breeding and breeding of precocious high-quality laying hens.

The invention relates to the technical field of plant molecular genetic engineering, in particular to an FvemiR172c gene for regulating and controlling anthocyanin accumulation of strawberry fruits and application of the FvemiR172c gene. The nucleotide sequence of the FvemiR172c gene is as shown in SEQ ID NO. 1 (sequence identifier number 1). The invention discloses a nucleotide sequence of an FvemiR172c gene for regulating and controlling the anthocyanin content of strawberry fruits, and experiments show that the FvemiR172c gene or an expression vector containing the FvemiR172c gene can be applied to regulation and control of the anthocyanin content of the strawberry fruits so as to regulate and control the color of the strawberry fruits. The invention provides a new gene resource for creating different colors of strawberries and molecular breeding for promoting / inhibiting anthocyanin accumulation.

The invention discloses a molecular genetic marker YZU-GOOSE-OE-1 for a goose eye opening character and an application of the molecular genetic marker YZU-GOOSE-OE-1. The molecular genetic marker comprises a GG genotype and / or a TT genotype, and the molecular genetic marker of the GG genotype is a sequence located at the SNP1 site of the FREM1 gene; the molecular genetic marker of the TT genotype is a sequence located at the SNP3 site of the PTPRM gene; the login number of the FREM1 gene is NC089912, and the login number of the FREM1 gene is NC089912; and the login number of the PTPRM gene is NC089874. According to the method, the selection accuracy of the goosefoot phenotype of the goosefoot is improved, the breeding population scale is reduced and the breeding cost is reduced by utilizing the molecular genetic marker obviously associated with the goosefoot character, and on the basis, the sex-associated genetic characteristic of the goosefoot character is utilized to cultivate the goosefoot character auto-sex specialized line, so that compared with phenotype selection, the cultivation process is obviously accelerated.