474 results about "Molecular genetics" patented technology

A resistance gene TaCAT to FCR of wheat and an application thereof are provided, which relates to the field of biological genetic engineering technologies. The nucleotide sequence of a genome where the TaCAT gene is located, the nucleotide sequence of a cDNA, the nucleotide sequence of a CDS and a coded protein sequence are respectively as shown in SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 and SEQ ID NO: 4. An InDel marker is developed for identifying different allelotypes of the TaCAT gene. An application of the TaCAT gene for preparation of transgenic plant cells and plant breeding is provided. The molecular genetic basis of the resistance to FCR is disclosed, an important role is played in improving the resistance to FCR by utilizing a genetic engineering technology, and a new way is provided for breeding a new wheat variety with disease-resistance and safety.

The invention relates to the technical field of molecular genetic breeding, in particular to a KASP molecular marker related to salt tolerance in a mung bean germination period, a primer group, a kit and application. According to the KASP molecular marker Chr126769484 related to the salt tolerance in the mung bean germination period, primers are designed according to the KASP molecular marker, typing is carried out by utilizing specific matching of basic groups at the tail ends of the primers, the KASP molecular marker related to the salt tolerance in the mung bean germination period is developed, genetic typing is carried out, identification is accurate and convenient, the salt tolerance in the mung bean germination period can be predicted at low cost, and the application prospect is wide. The process of salt-tolerant variety breeding in the mung bean germination period is simplified, the breeding efficiency is improved, and the mung bean breeding process is accelerated.

The invention discloses an STON2 gene intron SNP (Single Nucleotide Polymorphism) molecular marker related to egg laying traits of chickens and application of the STON2 gene intron SNP molecular marker, and belongs to the technical field of molecular genetics. The research finds that the STON2 gene may participate in the related process of poultry sexual precocity and egg laying, so that the reproductive performance and the egg laying character of the chicken are influenced, and four SNP loci remarkably related to the egg laying character of the chicken are found in the fifth intron region and the sixth intron region of the STON2 gene for the first time. Based on the SNP site, the invention further develops an SNP molecular marker related to the egg laying traits of the chickens, and the SNP molecular marker can be used for auxiliary breeding of high-yield laying hens.

The invention relates to the field of molecular genetics, in particular to a cynomolgus monkey specific SNP site combination, a liquid chip and application of the cynomolgus monkey specific SNP site combination and the liquid chip, 1508 cynomolgus monkey specific SNP sites disclosed and screened by the invention have high polymorphism, and rapid identification, paternity identification and lifelong tracking identification of cynomolgus monkey individuals can be realized. Liquid phase chip design is carried out on the sites, chip inspection is carried out on 383 cynomolgus monkey samples from three different groups, the result shows that the average site detection rate is 99.47%, and it is indicated that the liquid phase chip has excellent site capturing efficiency. According to the method, the genetic detection capability and accuracy of the cynomolgus monkeys can be greatly improved, the genetic detection cost can be remarkably reduced, and an important technical support is provided for improving the breeding management of the cynomolgus monkeys and optimizing the population structure.

The invention belongs to the field of SNP (Single Nucleotide Polymorphism) detection, and particularly provides an SNP molecular marker related to a sheep body length character, application of the SNP molecular marker and a kit for detecting the SNP molecular marker. The kit provided by the invention is used for performing polymorphism detection on the SNP molecular genetic marker, namely the 92690768th site of the No.4 chromosome, screening and determining the sheep body length character, and selecting the body length character to breed or assist to breed a long sheep individual with the body length character, so that a Tibetan sheep variety germplasm resource with high-quality body length character can be collected, and the kit has a very good application prospect.

The invention relates to the technical field of crop molecular genetic markers, and discloses an InDel molecular marker of avena nuda and application thereof.The molecular marker is a group of 314bp deletion variation and 2bp insertion variation in the 610439484-610440026 interval of the 5C chromosome position of a reference genome of an avena nuda variety OT3098, the nucleotide sequence of the deletion variation is shown as SEQ ID NO.1, the nucleotide sequence of the insertion variation is shown as SEQ ID NO.2, and the nucleotide sequence of the insertion variation is shown as SEQ ID NO.3. The nucleotide sequence of the insertion variation is 5 '-AA-3'. The molecular marker provided by the invention can realize stable detection in agarose gel electrophoresis by combining a specific primer pair (SEQ ID NO.2-3) with an optimized touchdown PCR (Polymerase Chain Reaction) program, has the advantage of being not influenced by a tissue part and a growth period, and provides an efficient and reliable technical means for oat germplasm identification, seed purity detection and molecular breeding.

The invention discloses a method for screening and identifying a cucumber aphid-resistant gene as well as a screened gene and application thereof, and provides a brand-new method for screening and identifying the cucumber aphid-resistant gene, which is used for screening the cucumber aphid-resistant gene based on four omics technologies. The four types of omics comprise transcriptome, proteome, methylation group and metabolome before and after inoculation of aphids on cucumbers, and the cucumber aphid resistance regulation gene is screened through combined analysis, so that a new gene resource is provided for improving the aphid resistance of the cucumbers, and a new method and a new idea are provided for designing molecular genetic breeding. The CsPGD2 gene obtained through screening is an aphid resistance gene, the dynamic change of a metabolic substance 5-ribulose phosphate in a metabolome is consistent with that of a transcriptome gene CsPGD2, the aphid resistance function of the gene CsPGD2 is verified through VIGS, and the result proves that the gene CsPGD2 is an important regulation factor for the aphid resistance of cucumbers. The invention provides a new gene resource for improving the aphid resistance of cucumbers, and has a good application prospect.

The invention provides a functional molecular marker of a wheat leaf rust resistance gene Lr.ace-4A, a detection method and application of the functional molecular marker. The functional molecular marker comprises an upstream primer as shown in SEQ ID NO: 1 and a downstream primer as shown in SEQ ID NO: 2. The method can solve the problem that the Lr.ace-4A gene in wheat is difficult to rapidly detect in the prior art, and is suitable for the field of molecular genetic breeding.

The invention discloses an InDel molecular marker related to wheat yield traits and application of the InDel molecular marker, and belongs to the technical field of molecular genetics. The InDel molecular marker is a molecular marker InDel 509; the nucleotide sequence of the molecular marker InDel 509 is as shown in SEQ ID No. 1. A wheat TaPRR95-5A gene functional marker InDel 509 is used for carrying out PCR (Polymerase Chain Reaction) amplification, polyacrylamide gel electrophoresis detection is carried out, and a germplasm material carrying a Hap II type is screened out according to the size of a target band. The functional marker can provide an effective marker resource for wheat genetic improvement and molecular marker-assisted breeding.

The invention discloses a KASP molecular marker related to brassica napus quality traits and application of the KASP molecular marker, and belongs to the technical field of molecular genetics. According to the KASP molecular marker related to the brassica napus quality traits, the primers of the KASP molecular marker and the application of the KASP molecular marker and the primers in brassica napus genetic typing or quality traits identification, results show that the KASP molecular marker can accurately identify genotypes, is high in sensitivity and specificity and effectively reduces the error rate. The invention also discloses a brassica napus genotyping / quality identification method, and the result shows that the material with the genotyping result of AA has the characteristics of low erucic acid content, low thioglycoside content, high oleic acid content, high linoleic acid content, low arachidonic acid content and low protein content; gG-type materials exhibit high erucic acid, high thioglycoside, low oleic acid, low linoleic acid, high arachidonic acid and high protein content. Individuals with excellent quality characters can be rapidly screened in early generations, and the long process of character identification after plant maturation in traditional breeding is avoided.

The invention relates to the technical field of molecular genetics, and particularly discloses a Yr6 gene, protein, a recombinant vector and application of the Yr6 gene, the protein and the recombinant vector, the full-length sequence of the Yr6 gene is shown as SEQ ID NO.1, and the CDS sequence of the Yr6 gene is shown as SEQ ID NO.2. Yr6 is overexpressed in a transgenic mode to improve wheat stripe rust resistance. The Yr6 gene provided by the invention is beneficial to improving the resistance of wheat to stripe rust, and provides support for breeding of stripe-rust-resistant wheat.

The present disclosure pertains to the biological field, specifically to a combination of 33 single nucleotide polymorphism (SNP) loci associated with the wool fiber diameter of fine wool sheep, determined using Ovis aries reference genome version 4.0. It also covers the use of reagents for detecting genotypes of these SNP loci in wool fiber diameter analysis or molecular marker-assisted selection of fine wool sheep. Additionally, it includes molecular probe combinations, gene chips, and kits based on the SNP loci. These tools facilitate early-stage wool fiber trait analysis, enabling individual selection, reducing generation intervals, accelerating breeding processes, and lowering costs. This disclosure provides crucial support for the identification, breed preservation, and molecular genetic breeding of fine wool sheep.

The invention relates to the technical field of crop molecular genetic markers, and discloses an Indel molecular marker of avena nuda and application of the molecular marker, the molecular marker is insertion variation of 551bp located at the 4D chromosome position 451669339-451669889 of a reference genome of an avena nuda variety Sanfensan, and the insertion sequence is as shown in SEQ ID NO. 1. The Indel molecular marker provided by the invention is completely related to the naked oat skin character, can be efficiently used for naked oat germplasm resource identification, variety purity detection and molecular marker-assisted breeding, remarkably improves the screening efficiency of naked oat excellent germplasm, and provides a brand new molecular detection technology for genetic improvement of the naked oat skin character. According to the invention, not only is a rapid and accurate technical standard established for naked oat variety identification, but also important molecular marker resources and application schemes are provided for oat quality improvement and germplasm innovation.

The invention discloses a molecular marker combination related to a cockscomb height character and a detection reagent and application thereof, and belongs to the technical field of molecular genetics. According to the molecular marker combination and the detection reagent thereof, ten SNP loci remarkably associated with the chicken first-laying crown height and the 130-day-old crown height are identified in the intron of the CTCF gene for the first time, based on the SNP loci, the molecular marker combination related to the cockscomb height character and the detection reagent thereof are designed, and the molecular marker combination and the detection reagent thereof can be used for early screening of laying hens with high cockscomb height and can be used for early screening of laying hens with high cockscomb height. The method is of great significance to breeding and breeding of precocious high-quality laying hens.

The invention discloses a molecular genetic marker YZU-GOOSE-OE-1 for a goose eye opening character and an application of the molecular genetic marker YZU-GOOSE-OE-1. The molecular genetic marker comprises a GG genotype and / or a TT genotype, and the molecular genetic marker of the GG genotype is a sequence located at the SNP1 site of the FREM1 gene; the molecular genetic marker of the TT genotype is a sequence located at the SNP3 site of the PTPRM gene; the login number of the FREM1 gene is NC089912, and the login number of the FREM1 gene is NC089912; and the login number of the PTPRM gene is NC089874. According to the method, the selection accuracy of the goosefoot phenotype of the goosefoot is improved, the breeding population scale is reduced and the breeding cost is reduced by utilizing the molecular genetic marker obviously associated with the goosefoot character, and on the basis, the sex-associated genetic characteristic of the goosefoot character is utilized to cultivate the goosefoot character auto-sex specialized line, so that compared with phenotype selection, the cultivation process is obviously accelerated.

The invention discloses an Indel marker related to a corn kernel protein content character and application of the Indel marker, and belongs to the technical field of plant molecular genetics and crop molecular design breeding. The Indel marker is located on a gdh2 gene of a chromosome 10 of a corn genome, and insertion or deletion of a nucleotide sequence as shown in SEQ ID NO.5 occurs in an intron region between a second exon and a third exon. By applying the molecular marker-assisted selection technology, high-protein new germplasms S3317 (the crude protein content is 17.0%) and JS231148 (the crude protein content is 15.6%) have been successfully cultivated, the increase amplitudes of the high-protein new germplasms and JS231148 are both more than 15% compared with those of the conventional control, and the lysine content reaches 0.41% and reaches the national high-quality protein corn standard.

The invention provides a DGAT1 gene SNPs marker for detecting sheep milk production traits and application thereof.Nucleotide sequence variation sites of the DGAT1 gene are detected with the first-filial generation of Dongfudrisheng sheep male * Hu sheep female as an object, a general linear mixing effect model is constructed, the correlation between nucleotide sequence variation and sheep milk production traits is researched, and the DGAT1 gene SNPs marker for detecting the sheep milk production traits is obtained. And digging a molecular genetic marker for regulating and controlling the lactation character of the sheep. Three SNPs (single nucleotide polymorphisms) sites are found in total; wherein two variation sites in the first intron are named as c.191 + 411 Cgt, and the second intron is named as c.191 + 411 Cgt; t (SNP1) and c.192-440 Cgt, and c. 292-440 Cgt; t (SNP2) and a variation site in the 17th exon are named as c.1461 Cgt; t (SNP3). For the SNP1 site, the average daily milk yield of the CT genotype ewe is increased by 8.2% and 17.04% compared with that of the CC genotype ewe and that of the TT genotype ewe respectively; for the SNP3 site, the milk fat percentage of the CC genotype ewe is improved by 0.314% compared with that of the CT genotype ewe. A new molecular marker can be provided for selection and improvement of lactation traits of the milk sheep, and the molecular marker has important application prospects in sheep screening or breeding.

The invention discloses an SNP (Single Nucleotide Polymorphism) marker related to a soybean branching number gene QNE1 and application thereof, and relates to the technical field of molecular genetics and crop genetic breeding, the SNP molecular marker is a nucleotide sequence as shown in SEQ ID NO.1 formed by single nucleotide mutation at the 686th site of the nucleotide sequence of the QNE1 gene in a soybean genome; the polymorphism of the single nucleotide mutation is C / G polymorphism. The method is used for assisting soybean breeding. The SNP marker provided by the invention can be used for molecular marker-assisted selection of soybean branch number related traits, and a key tool is provided for cultivating soybean varieties adapting to different planting requirements (such as a few-branch close planting type or a multi-branch high-yield type).

The invention discloses application of an SNAC1 gene in improving resistance of rice to rice blast. The nucleotide sequence of the SNAC1 gene is as shown in SEQ ID NO. 1, and the amino acid sequence coded by the SNAC1 gene is as shown in SEQ ID NO. 2. And the SNAC1 gene is used for editing a strain. The resistance of the rice to the rice blast can be obviously improved. The invention provides a basis for the key molecular genetic mechanism of rice blast resistance, and provides important guidance for cultivating resistant rice varieties and improving rice quality.

The invention belongs to the field of molecular genetics, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker combination for genetic relationship identification of wuzhu cattle and application of the SNP molecular marker combination. The SNP marker combination is composed of 140 high-polymorphism SNP sites distributed on a plurality of chromosomes of a bovine whole genome, the average distance between the sites is reasonable, and the SNP marker combination has good genome coverage. The selected SNP site has high allele frequency (MAF), expected heterozygosity (He) and polymorphic information content (PIC), and the cumulative exclusion probability is 0.9999. King and Plink software are combined to deduce a genetic relationship coefficient (Kinship) and an IBD shared value (PIHAT) between samples, and a result shows that the marker combination can realize a genetic relationship recognition effect equivalent to that of whole genome SNP data in Wuzhu white cattle (grassland white cattle), and the scientificity and practicability of the marker combination are verified. The SNP molecular marker combination can be widely applied to the processes of germplasm resource management, pedigree correction and cattle breeding, and has good popularization prospects and economic benefits.

The invention relates to a pathogenic gene identification method and system based on multi-agent debate and a medium. The method comprises the following steps: acquiring gene detection data and clinical phenotype data; the data agent processes the acquired data, and calls a gene variation pathogenicity analysis operator and a molecular genetic large model to obtain first pathogenic gene information; the knowledge agent calls a molecular genetic large model to obtain second pathogenic gene information based on the first pathogenic gene information; the knowledge and data agents sequentially speak and debate pathogenicity of candidate pathogenic genes in the first or second pathogenic gene information on the basis of sorting results in the pathogenic gene information output by the knowledge and data agents; after each round of debate is finished, the debate agent judges whether the sorting results of the data and the knowledge agent on the candidate pathogenic genes are consistent or not, if the sorting results are consistent or the number of debate rounds reaches a threshold value, debate is finished, the debate agent conducts reasoning and outputs a result, and if not, the next round of debate is started. Compared with the prior art, the method has the advantages of no dependence on large-scale training data, high interpretability and the like.

The invention relates to the technical field of animal molecular genetics, molecular marker breeding and aquatic genetic breeding, in particular to a Koi-TAT gene related to golden and silver scale traits of fancy carp, an SNP molecular marker, a primer pair and application. According to the invention, a functional gene highly associated with the Koi gold and silver scale character is found, a specific molecular marker and a detection method are developed on the basis of the gene, and by utilizing the molecular marker and the detection method disclosed by the invention, an individual with excellent gold and silver scale character can be efficiently identified, and the molecular marker is suitable for molecular-assisted selective breeding and early-stage seed selection. The invention provides an important molecular tool for phenotype genetic improvement of aquarium fish, also provides a basis for molecular mechanism research of gold and silver scale formation, and has a wide application prospect.

The invention provides a KASP molecular marker related to mesosulfuron resistance of wheat and application of the KASP molecular marker, and belongs to the technical field of crop molecular genetic breeding. Through correlation analysis, it is found that the KASP molecular marker is remarkably correlated with mesosulfuron-methyl resistance, T / C polymorphism exists, and the mesosulfuron-methyl resistance of CC homozygous wheat is remarkably higher than that of TT homozygous wheat. On the basis, a KASP labeling reagent or kit containing a specific primer group is developed, the primer group comprises two allele specific upstream primers and a universal downstream primer, and the 5'ends of the upstream primers are connected with FAM and HEX fluorescent labels respectively. By optimizing a PCR reaction system and a landing procedure, high-throughput genotyping can be realized. The mesosulfuron-methyl-resistant wheat variety can be rapidly screened by applying the KASP molecular marker, a technical support is provided for herbicide resistance molecular breeding, the breeding efficiency of the resistant variety is remarkably improved, and the KASP molecular marker has important significance for guaranteeing safe production of wheat.

The invention belongs to the technical field of soybean molecular genetic breeding, and discloses a KASP marker for detecting an SNP site related to a soybean petiole included angle and application of the KASP marker. The SNP site is located at the 4589285th site on a chromosome 10 of a soybean reference genome Glycine max Wm82. A4. V1, and the site is subjected to C / T basic group replacement. Three KASP primers as shown in SEQ ID NO.2-4 are developed according to the SNP site, PCR amplification and genetic typing are performed on to-be-detected soybeans by using the primer group, and if a detection result shows that the genetic typing of the marker position is 1 / 1, it is judged that the petiole angle of the soybean variety is large; and if the detection result is 0 / 0, judging that the petiole angle is small. The SNP molecular marker can be used for molecular marker-assisted selection of soybean petiole included angle characters, and has important theoretical and practical guiding significance for accelerating genetic improvement of soybean compact plant types and improving breeding selection efficiency. The marker can rapidly identify the soybean petiole included angle, the method is simple and rapid, the identification result is accurate, and the marker has good popularization and application prospects.

The invention discloses a plant gene function analysis method (FAGEN), and belongs to the technical field of molecular genetics and functional genomics. The method comprises the following steps: constructing a target gene mutant by using a gene editing technology; hybridizing the mutant and the corresponding wild type as a common parent with a plurality of parents with different genetic backgrounds to construct a pair F1 group; an F2 group is obtained through selfing; and determining phenotypic data of target characters of F1 and F2 populations, and comparing phenotypic differences of filial generations of wild types and mutants under different genetic backgrounds to judge the functions of the target genes. The method has the advantages of simplicity in operation, flexibility in design, short construction period and low cost, and gene functions can be efficiently and accurately analyzed under various genetic backgrounds.

The invention discloses a molecular marker closely linked with peanut yield related traits and application, and belongs to the technical field of plant molecular genetics and molecular breeding. The molecular marker disclosed by the invention is Arahy0036; the nucleotide sequence of Arahy0036 in peanuts with low single plant yield is shown as SEQ ID NO: 1, and the nucleotide sequence of Arahy0036 in peanuts with high single plant yield is shown as SEQ ID NO: 2. The marker is used for detecting the gene locus related to the yield character of the peanut single plant, the single plant with high yield can be identified after seeds are harvested, other single plants are selected, reserved and eliminated, the breeding scale can be effectively controlled, and the progress and efficiency of variety selection breeding are improved.

The invention is suitable for the technical field of molecular genetics, and provides an SNP molecular marker related to pig backfat thickness and application thereof. The invention studies and determines that the pig backfat thickness related molecular marker is located at the 95583333rd site (corresponding to the 701st basic group A / G mutation of SEQ ID NO: 1) of the No.18 chromosome of the international pig reference genome version 11.1, and verifies the influence effect of the molecular marker on the pig backfat thickness character. And when the basic group at the site is A, the pig has lower back fat thickness. On the basis, a molecular marker-assisted selection breeding technology for rapidly improving the backfat thickness character of the pig is established, the frequency can be increased generation by generation by optimizing the dominant allele (A), the backfat thickness of the breeding pig is remarkably reduced, the genetic improvement process of commercial pig herds containing landrace and large white pig blood lineage is accelerated, and the breeding efficiency is improved; the sales profit is increased for enterprises, the core competitiveness is improved, and the economic benefits of commercial pigs are improved.

The invention belongs to the field of plant molecular genetics, and relates to a spirodela polymorphic microsatellite molecular marker primer and application thereof. The kit comprises 10 pairs of microsatellite primers which form 5 groups of duplex PCR systems, and the kit can be applied to population genetic diversity analysis of spirodela polyrhiza. The population genetic diversity analysis comprises the following steps: collecting a sample of spirodela polyrhiza to be identified; extracting spirodela polyrhiza genome DNA from the spirodela polyrhiza sample; taking the extracted DNA as a template, and carrying out PCR (Polymerase Chain Reaction) amplification on the DNA template by using the screened 10 pairs of microsatellite primers; carrying out electrophoresis detection on a PCR product, and then carrying out silver staining; the silver staining result is digitally analyzed, the UPGMA clustering analysis chart is drawn by genetic analysis software, the clustering analysis chart of the spirodela polyrhiza is identified according to the clustering analysis result, and the method has huge popularization and utilization value.

The invention relates to the technical field of livestock molecular genetic breeding, in particular to application of a molecular marker for grassland short-tailed sheep body weight character breeding, the molecular marker is a nucleotide sequence as shown in SEQ ID NO.1, and nucleotide at the 101bp site is G or A; the application refers to any one of the following steps: identifying the weight of the grassland short-tailed sheep; the body weight of the grassland short-tailed sheep offspring is improved. The SNP molecular marker obviously related to the body weight character is identified on the 24 # chromosome of the grassland short-tailed sheep for the first time. The molecular marker can be used for genotype detection in a young sheep stage or an embryo stage, and does not need to wait for stable phenotype when the sheep grow to an adult stage, so that the breeding cycle is shortened, and the genetic progress is remarkably accelerated. The technical blank in the field of grassland short-tailed sheep body weight character molecular breeding is filled, and a reliable molecular tool and technical support are provided for efficient, accurate and sustainable breeding of the breed.

The invention belongs to the technical field of plant molecular genetic engineering, and particularly relates to a FaAL6 gene related to strawberry fruit hardness and application of the FaAL6 gene. The nucleotide sequence of the FaAL6 gene is as shown in SEQ ID NO. 1. The invention discloses a nucleotide sequence of a FaAL6 gene for regulating and controlling the hardness of strawberry fruits and an amino acid sequence coded by the FaAL6 gene, and experiments show that the FaAL6 gene or a construct or a host cell containing the FaAL6 gene can be applied to regulating and controlling the hardness of the strawberry fruits. The invention provides a new gene resource for molecular breeding for delaying strawberry fruit softening and improving strawberry fruit storage and transportation performance.