38 results about "Iron deficient" patented technology

The invention is applicable to the technical field of bioengineering, and provides a coding gene for enhancing iron deficiency stress tolerance of plants and application. According to the soybean GmMYB14 transcription factor disclosed by the invention, the problem that the growth of soybeans is limited in an iron-deficient environment, particularly calcareous soil and soda saline alkali soil, is solved by excavating and applying the encoding gene (the nucleotide sequence is shown as SEQ ID No.1) of the soybean GmMYB14 transcription factor. Experiments prove that after the gene is over-expressed in arabidopsis thaliana and soybean, the tolerance of plants to iron deficiency stress can be remarkably enhanced. The invention provides a key gene resource for molecular breeding: the GmMYB14 effectively promotes the absorption and utilization of plants on iron by regulating and controlling the metabolic pathway of phenylpropane, not only can improve the planting adaptability of soybeans in low-iron soil, but also can improve the yield and quality of the soybeans, provides powerful technical support for enlarging the planting area of the soybeans in iron-deficient soil regions, and has a wide application prospect. The method is of great significance in relieving insufficient soybean productivity in China and improving the self-sufficiency rate.

The invention discloses a maltol iron crystal form DCVIII, a preparation method and application thereof, a pharmaceutical composition containing the crystal form, and application of the crystal form in preparation of drugs for treating diseases related to iron deficiency of adults. The iron maltol crystal form DCVIII disclosed by the invention has good physical and chemical stability, good humidity stability and relatively low hygroscopicity, provides guarantee for subsequent production and development of medicines, and has relatively high industrial development value.

The application discloses a pentosus pentosaceus and postbiotic compound thereof for tonifying qi, nourishing face and improving anemia, and belongs to the technical field of microbial fermentation. The application screens a pentosus pentosaceus, obtains the bacterial body and postbiotic by culture, and forms a compound of the postbiotic and extract of semen ziziphi spinozae. The compound exhibits a synergistic effect, specifically, compared with the iron deficiency model group, the movement and various blood indexes of rats are improved, and are close to the normal rats of the control group. The indexes of rats in the compound group are better than those of rats fed with probiotics, and rats fed with postbiotic or semen ziziphi spinozae alone, which indicates that the compound can promote the recovery of iron content and protein indexes in the blood of rats more quickly. The application has practical value for comprehensive utilization of the pentosus pentosaceus and postbiotic, and also has important value for tonifying qi, nourishing face and improving anemia.

The present invention pertains to a combination of 2 types of inulins and nutritional compositions comprising that combination of inulins for improving the bioavailability of iron and preventing or treating iron deficiency, in particular for infants, young children, and subjects in need thereof.

The present invention relates to the use of ligustrazine nitrone derivatives and pharmaceutical composition thereof for the treatment and prevention of diseases of diabetic complications, which include renal anemia. The ligustrazine nitrone derivatives have the structure of the general formula (I).Experimental results indicated that the ligustrazine nitrone derivatives can significantly increase the levels of serum iron and erythropoietin in STZ induced SD rats and spontaneously hypertensive rats, and can be used for treating and preventing renal anemia caused by decrease in renal EPO production and / or concomitant iron deficiency. Therefore, the ligustrazine nitrone derivatives can be made into various dosage forms with a drug carrier. The derivatives can be prepared into various dose forms together with drug carriers.

This invention discloses the application of the PbeARF2 gene, an ARF-type transcription factor in *Pyrus pyrifolia*, belonging to the field of bioengineering technology. The nucleotide sequence of the PbeARF2 gene is shown in SEQ ID NO.1. The applications include enhancing plant resistance to iron deficiency stress, promoting root acidification, increasing the activity of ferric reductase under iron deficiency stress, cultivating plant varieties with high resistance to iron deficiency stress, and increasing chlorophyll content in leaves under iron deficiency stress. This invention, through biological experiments, demonstrates for the first time the function of the PbeARF2 ARF-type transcription factor gene and its encoded protein in promoting iron absorption in plants under iron deficiency stress, laying a theoretical foundation for plant variety improvement and the creation of plant varieties with high iron deficiency resistance.

In an iron (II) citrate monohydrate, identified by the formula FeC6H6O7-H2O ( Ferrous citrate monohydrate or iron citrate (II) monohydrate), obtained according to a particular production method, citric acid and elemental iron are bound in a molecular complex where the iron is agglomerated in the citrate which moves to a cyclic form and the iron is bound to the O groups of the carboxylic groups 1 and 3 through ionic bonds, forming two enantiomers, D and L (R and S), both perfectly water-soluble and highly assimilable by the human body. Formula (L) & Formula (D). With a different absorption mechanism from traditional iron molecules, said iron citrate monohydrate is usable in pharmaceutical and / or nutraceutical and / or food compositions, in particular in the treatment and prevention of iron deficiency anemias and in general of all diseases and disorders associated with iron deficiency. The effectiveness is proven by several examples.

The application belongs to the technical field of bioengineering, and provides an encoding gene for enhancing the tolerance of plants to iron deficiency stress and application thereof. The application solves the problem that soybeans are restricted in growth in an iron deficiency environment, especially in calcareous soil and soda saline-alkali soil, by mining and applying the encoding gene (nucleotide sequence as shown in SEQ ID No. 1) of a soybean GmMYB14 transcription factor. Experiments prove that the gene can significantly enhance the tolerance of plants to iron deficiency stress after overexpression in Arabidopsis thaliana and soybeans. The application provides a key gene resource for molecular breeding: GmMYB14 effectively promotes the absorption and utilization of iron in plants by regulating the phenylpropanoid metabolic pathway, which not only can improve the planting adaptability of soybeans in low-iron soil, but also can improve the yield and quality of soybeans, thereby providing strong technical support for expanding the planting area of soybeans in iron deficiency soil areas, and having important significance for relieving the insufficient production capacity of soybeans in China and improving the self-sufficiency rate.

The present invention relates to the application of a coding gene for enhancing iron accumulation in plants and tolerating iron deficiency stress, belonging to the technical field of bioengineering. Knocking out the coding gene for enhancing iron accumulation in plants and tolerating iron deficiency stress shown in SEQ ID No: 1 in the sequence listing, that is, not expressing it in plants, the plants show tolerance to iron deficiency, and the plant is Arabidopsis thaliana. The specific operation is as follows: The coding gene for enhancing iron accumulation and tolerating iron deficiency stress shown in SEQ ID No: 1 in the sequence listing is knocked out in Arabidopsis thaliana by the method of T-DNA insertion. RCD1 ). The present invention enables the above-mentioned gene for enhancing iron accumulation in plants and tolerating iron deficiency stress not to be expressed in plants by gene knockout, so that the plant body shows tolerance to iron deficiency. The gene for enhancing iron accumulation in plants and tolerating iron deficiency stress of the present invention can provide gene resources and technical support for breeding crops tolerant to iron deficiency stress.

This invention provides methods for quantitatively determining the concentration of free iron and ferritin-bound iron in a sample. The method includes diluting a ferritin-containing sample with a diluent to create a slurry, processing the slurry through a crossflow filtration system equipped with a membrane of at least 500 kilodaltons molecular weight cutoff, collecting the permeate containing free iron and the retentate containing ferritin-bound iron analyzing the iron concentrations in the permeate and retentate using a suitable analytical technique and calculating the mass balance of the total iron content. The invention also provides an antibody for use in quantitating the amount of ferritin in a sample and methods of treating iron deficiency in a patient in need thereof.

In the ferric iron citrate monohydrate defined by the chemical formula FeC6H6O7 H2O (ferrous citrate monohydrate or ferric iron citrate monohydrate) obtained according to a specific production method, the citric acid and the elemental iron are combined in a molecular complex in which the iron is gathered in the citrate moving into a ring-like form and the iron is bound to the O groups of the carboxyls 1 and 3 through ionic bonds, forming two enantiomers D and L (R and S), both of which are completely water-soluble and highly absorbable by the human body. Formula (L) and Formula (D). With a different absorption mechanism than traditional iron molecules, said ferric iron citrate monohydrate can be used in pharmaceutical compositions and / or nutritional compositions and / or food compositions, in particular for the treatment and prevention of iron deficiency anemia and all diseases and conditions associated with iron deficiency. The effectiveness is demonstrated through several examples.

The present application relates to a kind of kits for detecting sows' nutritional iron deficiency by biomarker quantitative detection evaluation and application, including fluorescent reaction liquid A, fluorescent reaction liquid B, internal standard, negative control, nucleic acid release agent and quantitative reference product, the fluorescent reaction liquid B contains detection primer and probe, the detection primer includes upstream primer and downstream primer, the sequence of upstream primer is 5'-ATCTGGCTTGTAAACCAACA-3', the sequence of downstream primer is 5'-ATCTTAGTTGTGGATTCCGT-3', the fluorescent reaction liquid B also contains primer probe, probe sequence is 5'-TCACCGTCAATTTCGGCTCT-3', 5' end is labeled with reporter fluorescent group, and 3' end is labeled with quenching fluorescent group.The present application effectively improves the accuracy of sows' nutritional iron deficiency detection, and provides scientific guidance for sow nutrition.

This invention discloses a kit for constructing an iron-dependent growth environment for Acinetobacter baumannii and its application method, belonging to the field of biotechnology. The kit is specifically designed for Acinetobacter baumannii, and its core components include a 50 mmol / L sterile stock solution tube of 2,2′-bipyridine, a 2000 μmol / L sterile stock solution tube of FeCl3, and a user manual. It does not include general-purpose LB medium, thus avoiding the problem of high-temperature and high-pressure deterioration of core reagents and highlighting the innovative combination of dedicated reagents. This invention also provides the application method of the kit. By adding the kit stock solution to the autoclaved and cooled LB medium in a specific ratio, standardized growth environments with normal iron, iron deficiency, and iron enrichment can be rapidly constructed. Dynamic monitoring and verification of the reversibility of growth inhibition-recovery ensure that the iron environment is precise and controllable. The kit is easy to operate, has good reproducibility, and can achieve standardized construction of the iron environment for Acinetobacter baumannii. It is suitable for iron metabolism-related research such as functional analysis of iron uptake-related proteins, development of iron deprivation antibacterial strategies, and screening of iron-deficient strains.

The present invention discloses the use of a composition for preparing an iron-supplementing preparation. The composition comprises cornus officinalis and / or a cornus officinalis extract, and mangosteen and / or a mangosteen extract. By combining the active ingredients, the present invention provides a composition that can promote the body's absorption of iron by promoting intestinal iron absorption and / or inhibiting hepcidin, thereby providing an iron-supplementing effect. The composition can further be used to treat functional iron deficiency and other indications, providing a more effective preparation for improving iron deficiency. Furthermore, the preparation prepared using the composition also has the effects of regulating physical constitution, replenishing deficiency and invigorating qi, and regulating the body's health.

The present disclosure includes compositions possessing a recombinant H-ferritin homopolymer that has a plurality of H-ferritin subunits with an amino acid sequence of SEQ ID NO: 1. Methods for treating an iron-related condition in a subject and for treating a neurological or blood disorder in a subject with such compositions are also disclosed.

The present invention relates to iron complex compounds for subcutaneous use in a method of therapy, e.g., for iron deficiency, particularly of iron deficiency anaemia, in a companion animal and to pharmaceutical compositions for subcutaneous administration, comprising an iron complex compound and a pharmaceutically acceptable carrier. The present invention relates more particularly, although not exclusively, to iron octasaccharide complexes, pharmaceutical compositions comprising an iron octasaccharide complex, and the iron octasaccharide complexes for use in a method of therapy of iron deficiency, particularly of iron deficiency anaemia, in a human or non-human subject.

The invention discloses application of a rice iron deficiency tolerance gene OsIDT1 (Oryza sativa L. iron deficiency tolerance gene 1). The sequence of the iron-deficiency tolerance gene OsIDT1 is as shown in SEQ ID NO. 1. The gene can be applied in multiple aspects of promoting the iron element to be transferred from mature tissues to new tissues, improving the iron utilization efficiency of the rice and the like. The biological function of the iron-deficiency tolerance gene OsIDT1 in rice is found through a large number of experiments, and the iron element can be prevented from being transferred from a mature tissue to a new tissue by knocking out the gene, so that the iron utilization efficiency, the yield, the grain iron content and the like of the rice are reduced; the over-expression of the gene can promote the iron element to be transferred from mature tissues to new tissues, so that the iron utilization efficiency, the yield, the grain iron content and the like of the rice are improved.

The invention discloses pediococcus pentosaceus capable of tonifying qi, beautifying skin and improving anemia and a metagen complex thereof, and belongs to the technical field of microbial fermentation. Pediococcus pentosaceus is obtained through screening, thalli and metabiotics of the thalli are obtained through culture, the metabiotics and spina date seed extract form a compound, and the compound shows a synergistic interaction effect, and specifically, compared with an iron-deficiency model group, the movement and various blood indexes of rats are improved, and are close to those of normal rats of a control group. All indexes of rats in the compound group are superior to indexes of corresponding groups of experimental subjects fed with probiotics and independently fed with metabiotics or spina date seeds, and it is indicated that the compound can more quickly promote recovery of the iron content and protein indexes in rat blood. The pediococcus pentosaceus oral liquid has practical value for comprehensive utilization of the pediococcus pentosaceus and the metaplasts thereof, and also has important value in the aspects of tonifying qi, maintaining beauty and improving anemia.

The subject invention provides compositions and methods for treating and / or preventing iron deficiencies and iron chlorosis in plants. Compositions comprising an iron-capturing ingredient are contacted with plant and / or its surrounding environment in order to increase the amount of iron that is bioavailable to the plant.

Health authorities recommend the use of iron drops as a supplement for each infant at the age of 6 months and encourage and monitor their use until the age of 2 years. In addition, the use of iron drops becomes a long-term necessity in all systemic conditions that cause iron deficiency. Iron discoloration is quite common in children, especially in the infant period (0- 2 years) due to the structure of the milk teeth and the incomplete oral flora. The developed toothpaste for infants and children comprising phytic acid will eliminate iron-related discoloration of milk teeth.

The present invention relates to crystal form DCIII of compound I and a preparation method therefor, and the use of the crystal form in the preparation of a drug for treating iron deficiency-related conditions with or without iron deficiency anemia. The new crystal form of compound I provided by the present invention has the advantages of high solubility, good chemical stability, solid-state stability, mechanical stability and humidity stability, a simple preparation method, easy crystal form control, and a low production scale-up cost, etc., and is of significant value for the future development of the drug.

Health authorities recommend the use of iron drops as a supplement for each infant at the age of 6 months and encourage and monitor their use until the age of 2 years. In addition, the use of iron drops becomes a long-term necessity in all systemic conditions that cause iron deficiency. Iron discoloration is quite common in children, especially in the infant period (0- 2 years) due to the structure of the milk teeth and the incomplete oral flora. The developed toothpaste for infants and. children comprising bromelain will eliminate iron-related discoloration of milk teeth.

Injectable iron compositions comprising iron, a carbohydrate, a stabilizing agent and water are provided. These iron compositions can be prepared by mixing iron with a carbohydrate and water to form a mixture and adding a stabilizing agent to the mixture to form the stable injectable iron composition. These compositions can be considered “ready-to-use” and can treat a variety of diseases, disorders, or conditions characterized by iron deficiency or dysfunctional iron metabolism, for example, anemia.

The present invention relates to novel iron-containing pharmaceutical compositions and / or dietary supplement compositions and / or food compositions, and their use in the treatment and / or prevention of absolute or relative iron deficiency conditions in subjects in need thereof. Specifically, the present invention relates to novel compositions containing highly assimilable iron, and their use in the treatment and / or prevention of disorders and diseases associated with iron deficiency. The present invention also relates to methods for preparing said compositions.

The invention belongs to the technical field of bioengineering, discloses a pseudomonas putida purF gene knockout strain and application thereof, and particularly discloses application of a reagent for knocking out a purF gene or a reagent for inhibiting purF gene expression to improvement of riboflavin synthesis capacity of pseudomonas putida. The invention discloses that the iron deficiency adaptive capacity, the adhesion capacity and the biofilm generation capacity of the pseudomonas putida can be remarkably improved by knocking out or inhibiting the purF gene or the expression of the purF gene in the pseudomonas putida for the first time, and the expression of fish host iron balance related genes can be regulated and controlled, so that the iron content of fish host tissues is improved.

The application discloses a strain for significantly improving HSAF yield under iron limitation and application thereof, and the genetically engineered bacterium is obtained by knocking out or silencing an iron response transcription factor Fur gene of L.enzymogenes OH11, and the nucleotide sequence of the Fur gene is shown as SEQ ID NO.1.Compared with a wild type strain, the mutant strain has no significant difference in growth in an iron deficiency or low-iron culture medium, and the HSAF synthesis capacity is greatly improved.The HSAF yield of the mutant strain in the iron deficiency culture medium reaches 162.34+5.84 mg / L, which is 3.60 times of that of the wild type strain.Therefore, the technical scheme of the application provides a more direct and effective way for improving the HSAF yield of L.enzymogenes OH11 in the iron deficiency or low-iron environment.

The application belongs to the technical field of detection, and particularly relates to a primer and kit for detecting lactobacillus reuteri and application, which comprises an upstream primer and a downstream primer, the sequence of the upstream primer is 5'-AAGCACCCTGATCCGCAAT-3', and the sequence of the downstream primer is 5'-AATTTGCCGCGCTTCGTCT-3'. The application has high sensitivity, good specificity and high precision, and can effectively detect early nutritional iron deficiency of sows.

The present invention relates to novel iron-containing pharmaceutical and / or nutraceutical and / or food compositions, and their use in the treatment and / or prevention of absolute or relative iron deficiency conditions in individuals in need thereof. Specifically, the present invention relates to novel compositions containing highly assimilable iron, and their use in the treatment and / or prevention of disorders and conditions associated with iron deficiency. The present invention also relates to methods for preparing said compositions.