539 results about "Ferritin" patented technology

The invention belongs to the field of biological medicines, and particularly relates to a varicella-zoster virus gE fusion protein as well as a preparation method and application thereof. The varicella-zoster virus gE fusion protein comprises an amino acid sequence as shown in SEQ ID NO: 1 and at least one of the following fragments: a signal peptide, a Foldon sequence, a Ferritin sequence and a His tag sequence, the signal peptide can improve the protein expression quantity, the Foldon sequence can improve the stability of the protein, the particle size of an antigen can be increased, the immunogenicity of the antigen can be improved, and the fusion protein can be used for preparing a medicine for treating the varicella-zoster virus gE fusion protein. The Ferritin sequence can enhance immune response, a vaccine prepared from the gE fusion protein has excellent immunogenicity, and compared with a commercially available vaccine Shingrix, higher humoral immunity and cellular immunity levels can be induced.

The invention discloses a bovine I-type alpha interferon-ferritin fusion protein, a mutant thereof, a preparation method and an application of the bovine I-type alpha interferon-ferritin fusion protein. The bovine I-type alpha interferon is fused with a ferritin subunit, and interferon molecules are highly repeatedly and orderly displayed on the surface of a ferritin nanocage by utilizing the self-assembly characteristic of ferritin, so that the expression level, the structural stability and the antiviral activity of the interferon are remarkably improved. The fusion protein is further subjected to single-site or multi-site rational design mutation, and a mutant with significantly improved antiviral activity and stability is obtained. According to the invention, a silkworm or insect cell eukaryotic expression system is adopted to express the fusion protein or the mutant thereof, and the expression system is safe to operate, simple and convenient in procedure, low in cost and extremely beneficial to large-scale industrial production; the prepared fusion protein or mutant nanoparticles have application prospects in preparation of drugs or reagents for preventing or treating bovine viral diseases.

The invention discloses preparation and application of antioxidative, anti-inflammatory and hypoglycemic lactoferrin peptide, and belongs to the field of food science and nutriology. According to the invention, lactoferrin is used as a raw material, hydrolysis is carried out by using a composite enzymatic hydrolysis method, and the molecular weight is obtained by ultrafiltration; the preparation method comprises the following steps: preparing a component of antioxidant, anti-inflammatory and hypoglycemic peptide from the active peptide YLSW (Tyr-Leu-Ser-Trp), carrying out freeze drying on the component to obtain peptide powder rich in antioxidant, anti-inflammatory and hypoglycemic peptides, identifying peptide fragments contained in the peptide powder by utilizing an LC-MS / MS technology, and screening out the active peptide YLSW (Tyr-Leu-Ser-Trp) with antioxidant, anti-inflammatory and hypoglycemic triple functions. The active peptide is added into the product to obtain the antioxidant, anti-inflammatory and hypoglycemic functional product.

The invention relates to the technical field of hemodialysis monitoring, and provides a hemodialysis on-line monitoring system and method, and the system comprises a data acquisition module which is used for collecting blood physiological parameters in a dialysis pipeline in real time; the blood component analysis module is connected with a blood loop of the dialysis machine and is used for detecting serum iron, ferritin and ferritin saturation indexes on line; and the data processing module is used for calculating the risk index of anemia, evaluating the iron utilization rate, constructing a model for measuring the iron deficiency erythropoiesis trend according to the collected and analyzed data, and training and optimizing the model. According to the method, individual parameters of patients are fused, personalized calculation is performed for iron metabolism rates and dialysis parameters of different patients, and data such as iron content, blood flow and dialysis fluid volume are acquired online in real time, so that iron concentration and infusion rate are accurately matched with real-time iron metabolism requirements of the patients, and anemia of the patients in the dialysis process is reduced.

The invention discloses an oligopeptide composition for inhibiting inclusion body formation and promoting Gag-Like protein nanoparticle self-assembly, the oligopeptide composition comprises a C-terminal oligopeptide and an N-terminal oligopeptide, the amino acid sequence of the N-terminal oligopeptide is as shown in SEQ ID NO: 1, and the amino acid sequence of the C-terminal oligopeptide is as shown in SEQ ID NO: 2. The oligopeptide composition can effectively avoid formation of a prokaryotic expression protein inclusion body and / or promote dissolution of prokaryotic expression protein, the oligopeptide composition is further utilized to be combined with HIV-1p24 and human endogenous retrovirus K capsid protein CA, nanoparticles are prepared respectively, and the oligopeptide composition can be used as a carrier for antigen surface display or drug transportation. The nanoparticles formed by the HIV-1 capsid protein p24 and the human HERV K CA have cavities which are obviously larger than those of ferritin, so that the nanoparticles have higher loading capacity; as an antigen display platform, the antibody also has good stability and wide applicability.

The invention provides a micro-fluidic chip for combined detection of C-reactive protein and ferritin and application thereof.The micro-fluidic chip comprises a substrate and a cover plate pressed on the substrate, a micro-channel is defined by the substrate and the cover plate, the left end of the micro-channel is communicated with a buffer solution sample adding hole formed in the cover plate, the right end of the micro-channel is communicated with a waste liquid area, and the left end of the micro-channel is communicated with a buffer solution sample adding hole formed in the cover plate. The waste liquid area is provided with a movable water-absorbing paper sheet, the microchannel is sequentially provided with a marking area, a detection area and a sample adding area from left to right, the marking area is provided with a marked anti-human CRP antibody, a marked anti-human SF antibody, a marked second antibody and a marked antigen, the first detection site is provided with an anti-human CRP antibody, and the second detection site is provided with an anti-human SF antibody. The first reference point is provided with a first antibody which reacts with the labeled second antibody, the second detection site is provided with an anti-human SF antibody, and the second reference point is provided with an antibody which reacts with the labeled antigen. The detection process of C-reactive protein and ferritin can be simplified, and the detection time is shortened.

The present application discloses a fusion protein comprising a varicella zoster virus protein and a ferritin, a nucleic acid encoding the fusion protein, a pharmaceutical composition comprising the fusion protein, a method of preparing the fusion protein, and a use of the fusion protein.

The invention discloses a porcine epidemic diarrhea virus antigen fusion protein, a coding gene and a porcine epidemic diarrhea vaccine prepared from the porcine epidemic diarrhea virus antigen fusion protein. The method comprises the following steps: screening to obtain a homosensory sequence of the spike protein of the porcine epidemic diarrhea virus, and fusing the homosensory sequence with a monomeric porcine ferritin heavy chain subunit to obtain an antigen fusion protein; in order to improve the expression quantity or the titer of the antigen fusion protein, the obtained antigen fusion protein is subjected to sequence optimization and then subjected to single-site or multi-site mutation, and the expression quantity and the titer of the obtained mutant are remarkably improved. According to the invention, a recombinant protein is expressed by using a silkworm or AcMNPV-insect cell and other eukaryotic expression systems to obtain a self-assembled ferritin nanoparticle antigen for displaying an S protein antigen protein on the surface of a cage structure of porcine ferritin, and a vaccine prepared from the prepared nanoparticle antigen can induce a widely neutralized anti-porcine epidemic diarrhea virus antibody; the porcine epidemic diarrhea virus vaccine has the potential of becoming a universal porcine epidemic diarrhea virus vaccine with cross immune efficacy.

The invention discloses a grass carp gamma interferon fusion protein as well as a mutant, a coding gene and application thereof. In order to improve the expression quantity or expression efficiency of the grass carp gamma interferon, codon optimization and mutation are carried out on the coding gene of the grass carp gamma interferon to obtain the grass carp gamma interferon mutant. The fusion protein is further obtained by connecting the grass carp gamma interferon mutant with the C end of the grass carp ferritin heavy chain subunit of which the last 17 amino acids are removed through a connecting peptide. In order to further improve the antiviral activity of the fusion protein, the obtained fusion protein is subjected to single-point mutation, and the titer of the fusion protein is remarkably improved. The fusion protein is expressed by a silkworm cell eukaryotic expression system, and the fusion protein shows a conformation suitable for the interferon to play functions based on the ferritin self-assembly characteristic, so that the titer of the interferon in a host is effectively improved, and the in-vivo and in-vitro half-life period of the interferon is prolonged. The invention has application prospects in preparation of drugs or reagents for preventing or treating viral diseases of grass carp and the like.

Provided herein are isolated polynucleotides encoding a polypeptide comprising an antigen protein fused to a signal sequence and a transmembrane domain, and optionally fused to a ferritin. Also provided herein are isolated polynucleotides encoding alphaviral non-structural proteins nsp1, nsp2, nsp3, and nsp4 and polypeptides comprising an antigen protein fused to a signal sequence and a transmembrane domain, and optionally fused to a ferritin. The antigen may be influenza. Polynucleotides, such as RNAs, can be used as vaccines against influenza infection.

The invention provides an mRNA (messenger ribonucleic acid) vaccine and application of the mRNA vaccine in preparation of a medicine for treating tumors. The vaccine consists of ionizable lipid, cholesterol, phospholipid, a PEG (Polyethylene Glycol) derivative and mRNA (Messenger Ribonucleic Acid) containing a gene sequence for coding norovirus S protein fusion tumor antigen or a gene sequence for coding tumor antigen fusion ferritin. According to the mRNA vaccine designed by the invention, the antigen expression quantity is increased, a nanocrystallization antigen structure is formed to promote antigen processing and presentation, strong anti-tumor cell immune response is stimulated, and a tumor local microenvironment is effectively remodeled, so that a remarkable tumor inhibition effect is achieved.

This disclosure relates to fusion proteins comprising one or more EBV polypeptides and a ferritin protein, and their use in eliciting antibodies against EBV.

The invention relates to a human lactoferrin indirect competitive enzyme-linked immunosorbent assay kit and application thereof. The kit comprises an elisa plate, an anti-human lactoferrin antibody, an elisa secondary antibody, a confining liquid, a color developing agent and a stop solution. A nitrocellulose membrane in Western-Blot is replaced by an elisa plate with non-specific adsorption protein, and along with the addition of a sample, an anti-human lactoferrin antibody and an enzyme-labeled secondary antibody, a lactoferrin-primary antibody-secondary antibody compound is formed. And adding a color developing agent to carry out color development reaction, wherein the absorbance value of the sample is positively correlated with the content of the lactoferrin contained in the sample. And calculating the content of human lactoferrin in the sample according to the absorbance value. The method for detecting the human lactoferrin in the fermentation liquor has the advantages of reality, economy, high efficiency and the like.

The invention relates to the technical field of aquaculture, in particular to nile tilapia ferritin, a preparation method and application thereof and a bacteriostatic agent. The amino acid sequence of the nile tilapia ferritin is as shown in SEQ ID No.1, and the nile tilapia ferritin inhibits streptococcus agalactiae and / or aeromonas hydrophila. The invention provides Nile tilapia ferritin, and in-vitro experiments prove that the recombinant protein can effectively inhibit proliferation of pathogenic bacteria. A further in-vivo experiment research result shows that the ferritin heavy chain (Nile tilapia ferritin) has important functions of inhibiting pathogenic bacterium proliferation and improving the survival rate of an organism, and plays an important role in immune protection of the organism.

The present invention belongs to the field of biomedicine, and specifically relates to an antimicrobial peptide derived from lactoferrin and its application. The antimicrobial peptide derived from lactoferrin, whose amino acid sequence is shown in any one of SEQ ID NOs: 1 to 98, has a broad-spectrum antimicrobial effect, especially significant antibacterial effect against Pseudomonas putida, Rhodococcus erythropolis, Bacillus subtilis, Malassezia furfur, Cryptococcus neoformans, and Candida albicans, and has great application value.

The present invention discloses a complete medium for culturing DF-1 cells, including DMEM basal medium 13.7g / L, insulin, non-essential amino acids, transferrin, glutathione, ascorbyl phosphate, sodium selenite, ethanolamine, hydrocortisone, and newborn calf serum. Wherein non-essential amino acids include glycine, alanine, proline, serine, and asparagine. A complete medium for culturing DF-1 cells provided by the present invention can well promote the proliferation of DF-1 cells, can achieve the same proliferation effect as high-concentration fetal bovine serum, and is suitable for the adherent culture of DF-1 cells; using low-concentration newborn calf serum, it is possible to greatly avoid the batch differences, unstable culture results, cytotoxicity, and high risk of carrying pathogens brought by the culture medium with high-concentration serum, narrow application range, expensive fetal bovine serum, and high culture cost; the components are simple, the culture medium preparation process is short in time consumption, the culture efficiency is high, the repeatability is good, and it is suitable for batch production.

The invention discloses paenibacillus sp. H31, which is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC NO: M 20252669, and the preservation date is November 24, 2025. The invention further discloses a preparation method of the paenibacillus sp. The paenibacillus H31 disclosed by the invention not only has broad-spectrum disease-resistant activity on pathogenic fungi of various plants, but also has a remarkable growth-promoting effect on pepper, so that the paenibacillus H31 can be widely applied to prevention and treatment of agricultural diseases and insect pests, and sustainable development of green agricultural production is promoted. In addition, the paenibacillus H31 also has the effects of hydrolyzing proteinase and cellulose and decomposing ferritin, so that the paenibacillus H31 also can be applied to the scenes of industrial production and the like.

The invention provides a biomarker for diagnosing systemic lupus erythematosus and application of the biomarker. According to the application disclosed by the invention, the expression of Ferritin in neutrophil of a systemic lupus erythematosus patient is found to be obviously reduced and is improved after treatment, the neutrophil has disease specificity and cell specificity, and the Ferritin in the neutrophil has important significance on clinical diagnosis or auxiliary diagnosis of systemic lupus erythematosus and disease condition evaluation.

The invention discloses a method for separating and purifying bovine lactoferrin by using a ligand affinity chromatography technology, and belongs to the technical field of protein purification. The method comprises the following steps: introducing a bovine lactoferrin gene into pichia pastoris, fermenting to obtain a pichia pastoris fermentation solution, centrifuging, resuspending, homogenizing at high pressure, centrifuging and filtering the pichia pastoris fermentation solution to obtain a filtrate, separating and purifying bovine lactoferrin in the filtrate by using an affinity chromatography technology, immobilizing a triazine dye ligand in a filler of a chromatographic column, and purifying the triazine dye ligand to obtain the bovine lactoferrin. An eluent is prepared, the concentration of NaSCN in the eluent is 0.2-0.5 mol / L, the volume concentration of ethylene glycol in the eluent is 15%-20%, the concentration of NaCl in the eluent is 1-2 mol / L, and the bovine lactoferrin in the chromatographic column is eluted. According to the method, the composition of the eluent is optimized, the three components in the eluent are matched with one another and have a synergistic effect, the stability of the bovine lactoferrin is protected, the purity of the bovine lactoferrin is guaranteed, meanwhile, the elution efficiency of the bovine lactoferrin is improved, and the dosage of the eluent is reduced.

The invention relates to the technical field of antibacterial peptides, and discloses a preparation method and application of a multi-segment fused antibacterial peptide fusion protein. The antibacterial peptide fusion protein is obtained by introducing OST signal peptide and collagen and optimizing the connection sequence of the OST signal peptide, the collagen, bovine lactoferrin peptide LFcinB, bovine lactoferrin N-leaf small peptide LFcin and bovine lactoferrin N-leaf derived peptide Lf. The antibacterial peptide fusion protein can simultaneously contain three segments of bovine lactoferrin active peptides, namely LFcinB, LFcin and Lf, and the yield of the fusion peptide is maximized. Experiments prove that the fermentation yield of the fusion protein comprising the OST signal peptide, the collagen, the bovine lactoferrin peptide LFcinB, the bovine lactoferrin N-leaf small peptide LFcin and the bovine lactoferrin N-leaf derived peptide Lf which are sequentially connected from the N terminal to the C terminal can reach 600mg / L.

The invention discloses a pH-responsive deformable dual-targeting nano protein and a preparation method and application thereof, the method comprises the following steps: activating carboxylated mannose through an EDC / NHS system, reacting with an albumin solution, and dialyzing and purifying to obtain mannose modified albumin; and mixing the mannose modified albumin with an inhibitor IRG1-IN-1, and loading the IRG1-IN-1 through a hydrophobic effect to obtain the mannose albumin nanoparticles, ferritin serves as a carrier, oxaliplatin is loaded through a solvent replacement method, and ferritin nanoparticles are obtained; cHO-PEG2k-CHO is used as a coupling agent, and the mannose albumin nanoparticles and the ferritin nanoparticles are crosslinked through a Schiff base reaction, so that the large-size pH-responsive dual-targeting nano-protein is formed. The nano protein has the characteristic of double-targeting separability, so that specific treatment can be realized, immune cell metabolism is interfered while tumor cells are killed, metabolic immunosuppression is blocked, and anti-tumor immunotherapy is promoted.

The invention discloses a multifunctional nano delivery system based on targeted ferritin as well as a preparation method and application of the multifunctional nano delivery system. According to the system, traditional Chinese medicine active ingredients, namely neogambogic acid GNA, a photosensitizer Ce6 and ferritin targeting peptide HKN15 are integrated through a self-assembly technology, a synergistic treatment system integrating photodynamic therapy PDT and ferroptosis induction is constructed, and the treatment effect on non-small cell lung cancer NSCLC can be remarkably enhanced. According to the nano delivery system, precise targeting of a tumor site is achieved through the ferritin targeting peptide HKN15, and a double anti-tumor mechanism is formed by utilizing the photodynamic effect of the photosensitizer Ce6 and the ferroptosis induction effect of GNA. The preparation process is based on a molecular self-assembly principle, and has the advantages of high tumor accumulation efficiency, low system toxicity, remarkable tumor inhibition effect and the like, and an innovative solution is provided for precise treatment of the non-small cell lung cancer.

The present invention relates to the technical field of biomedicine. Specifically provided are a respiratory syncytial virus (RSV) Pre-F recombinant protein nanoparticle vaccine and a preparation method therefor. By performing amino acid mutation on an RSV Pre-F protein, and performing fusion expression on the mutated Pre-F protein and ferritin particles in eukaryotic cells, PreF-Ferritin fusion protein nanoparticles, in which eight Pre-F protein trimers are densely displayed on the surface of each ferritin particle, are obtained. By stabilizing and exposing antigen epitopes required to be displayed and disrupting or masking unrequired antigen epitopes, the PreF-Ferritin fusion protein nanoparticles effectively improve the immunogenicity and the production stability of the antigen. Experiments show that: when the PreF-Ferritin fusion protein is injected into mice, high-titer protective sera can be acquired, and the mice sera can yield relatively high neutralizing titers against RSV.

The invention relates to a preparation method and application of recombinant canine lactoferrin and peptide thereof, aiming at codon preference of host cells, canine lactoferrin genes are optimized, the full length of canine lactoferrin and the lactoferrin peptide are successfully expressed by virtue of an escherichia coli expression system, and the recombinant canine lactoferrin and the peptide thereof are identified by virtue of a minimum inhibitory concentration experiment. It is proved that the canine lactoferrin and the peptide thereof have the inhibiting capacity on pathogenic bacteria such as staphylococcus aureus, salmonella typhimurium and staphylococcus pseudointermedius, and the antibacterial activity MIC of the canine lactoferrin on the staphylococcus aureus, the salmonella typhimurium and the staphylococcus pseudointermedius is 1.5 mg / mL; the antibacterial activity MIC of the dog lactoferrin peptide to staphylococcus aureus is equal to 0.75 mg / mL, and the dog lactoferrin peptide shows high antibacterial ability.

The invention relates to the technical field of biological medicine, in particular to a nano-drug with a dual targeting effect and a preparation method and application thereof, and the nano-drug comprises exosome and ferritin; the surface of the exosome is modified with an azide group, the surface of the ferritin is modified with a cyclopentyl group, and the exosome and the ferritin are connected through a click chemical reaction between the azide group and the cyclopentyl group. According to the nano-drug, the drug carrying efficiency is improved through the exosome and the ferritin, meanwhile, the nano-drug can effectively penetrate through a cerebral blood barrier and is taken by glioma cells, and the killing effect of the drug on tumor cells is enhanced.

The invention discloses a method for preparing a Clostridium perfringens epsilon toxin-ferritin nanoparticle antigen, comprising constructing a soluble E toxin mutant and a soluble Ferritin protein respectively and purifying them, and then self-assembling the soluble E toxin mutant and the soluble Ferritin protein to form a nanoparticle antigen. The epsilon-ferritin nanoparticles prepared by the invention significantly promote the production of IgG in antigen-immunized mice and successfully induce a cellular immune response.

The invention provides a preparation method and application of macronuclear magnetic ferritin, and the method is as follows: synthetic ferritin is subjected to multiple biomimetic mineralization, large-particle-size nanoparticles are formed in the ferritin, and finally, the macronuclear magnetic ferritin is obtained through separation and purification. The average particle size of the macronuclear magnetic ferritin prepared by the method is not less than 7nm, the magnetocaloric performance is remarkably improved, and the magnetic hyperthermia curative effect taking the magnetic ferritin as a thermal medium can be enhanced.

The invention discloses a method for preparing low-bitterness micromolecular bovine colostrum protein peptide powder by adopting a multi-stage membrane separation and enzyme membrane coupling combined technology, and belongs to the technical field of food processing. The preparation method comprises the following steps: firstly, dividing a colostrum component into components to be hydrolyzed by adopting a multi-stage membrane separation technology, namely casein intercepted by ceramic membrane microfiltration and lactoglobulin and lactalbumin filtered by an ultrafiltration membrane, and non-hydrolytic components, namely immune globulin and lactoferrin; the preparation method comprises the following steps: firstly, separating bovine coloctrum, then deeply hydrolyzing the separated lactoglobulin, milky white and casein flocculate with the molecular weight of less than 50kDa by adopting an enzyme membrane coupling technology, mixing with unhydrolyzed immunoglobulin and lactoferrin, and freeze-drying, so that the peptide content in the treated bovine coloctrum is more than 40%, the peptide content of 200-1000Da is more than 30%, the peptide content of 200-1000Da is more than 30%, and the peptide content of 200-1000Da is more than 30%. The preparation method is simple, the preparation dispersion time is within 10 s, no obvious bitter taste exists, the retention rate of immune globulin in bovine coloctrum reaches 90% or above, and the preparation method is suitable for large-scale industrial popularization.

The invention relates to the technical field of food processing, in particular to antioxidant nano collagen peptide powder and a preparation method thereof.The antioxidant nano collagen peptide powder is prepared by forming antioxidant micelles wrapping collagen peptide through collagen peptide liquid and a complex antioxidant, loading the antioxidant micelles on a porous carrier and then conducting cross-linking through transglutaminase; tea polyphenol and whey protein are covalently combined to prepare a composite antioxidant, rapid free radical neutralization, lactoferrin inhibition of an oxidation source and synergistic effect of collagen peptide self-antioxidant groups are provided, multistage barrier design is combined, oxygen diffusion is isolated through a micelle hydrophobic core, and the contact area of micelles and the outside is reduced through physical adsorption of a porous carrier, so that the anti-oxidation effect of the collagen peptide is improved. Finally, the micelles are fixed in the carrier by utilizing an enzyme cross-linked network, so that the antioxidant nano collagen peptide powder has efficient and long-acting antioxidant capacity.

The invention provides a porcine deltacoronavirus S1 protein nanoparticle vaccine CTDnps and application thereof, and belongs to the technical field of vaccine preparation. The PDCoV S1 protein C-terminal structural domain nanoparticle vaccine capable of being self-assembled, provided by the invention, comprises ferritin and a PDCoV S1 protein C-terminal structural domain, the amino acid sequence of the ferritin is as shown in SEQ ID NO.1, and the amino acid sequence of the PDCoV S1 protein C-terminal structural domain is as shown in SEQ ID NO.2. The PDCoV S1 protein C-terminal structural domain nanoparticle vaccine capable of being self-assembled comprises the ferritin and the PDCoV S1 protein C-terminal structural domain. The PDCoV S1 protein C-terminal structural domain nanoparticle vaccine (S1CTD-Fer) constructed by the invention can be self-assembled to form ferritin-like cage-shaped nanoparticles, and the hydrodynamic diameter of the cage-shaped nanoparticles is obviously greater than that of natural ferritin nanoparticles. Compared with the S1CTD, the S1CTD-Fel based on the ferritin carrier can obviously enhance the immunogenicity of the S1CTD, can obviously enhance the uptake efficiency of antigen presenting cells, and is high in safety.