15 results about "Tributyrin" patented technology

The application discloses a Pseudomonas atacamensis STR8 and an application thereof, and belongs to the technical field of microorganisms. The strain is classified and named as Pseudomonas atacamensis STR8, and the preservation number is CCTCC NO: M 2025609. The hydrolysis circle HC value of the strain on a tributyrin plate is 2.78, and the lipase activity reaches 139.86 U / mL. The application also provides an application of the strain and a lipase produced by the strain in catalyzing hydrolysis of tocopherol succinate to prepare tocopherol. The strain STR8 is fermented to obtain a crude enzyme liquid, and the crude enzyme liquid is added into a reaction system containing the tocopherol succinate to perform a hydrolysis reaction. After 9 hours of reaction, the content of the tocopherol is increased by 750% compared with a control group. The strain and the method provided by the application have high catalytic efficiency, mild reaction conditions and environmental friendliness, and are suitable for green industrialized production of the tocopherol.

The present application relates to a kind of fat powder for reducing weaning piglet dead rate and its preparation method and application, and the composite lipid in the preparation raw material of fat powder includes 1,3-di-oleic acid-2-palmitic acid triglyceride, 24 degrees palm oil, rice bran oil, coconut oil, linseed oil, caprylic triglyceride, capric acid triglyceride, alpha-lauric acid monoglyceride and tributyrin.The present application develops the fat acid composition close to pig milk fat structure, let piglet continue lactation period in weaning period to enable approach mainly with lipid, meet the compound energy demand of fast, medium and slow, avoid falling into energy potential difference;And the specific combination of medium and short chain fatty acids, both can improve the energy efficiency of weaning piglet, also have bacteriostatic disease characteristics, can improve the immunity of piglet, reduce the diarrhea rate.This composite lipid overcomes the problem such as piglet energy deficiency and indigestion caused by single raw material oil due to unsuitable lipid structure, can significantly reduce weaning stress, reduce the diarrhea rate and dead rate.

The compositions and methods for improving or regulating GLP-1 levels, stimulating GLP-1 secretion, ameliorating metabolism including losing weight, managing glucose or insulin tolerance including stimulating insulin secretion, inhibiting glucagon secretion and reducing fasting glucose levels. The compositions comprise β-aminoisobutyric acid, a physiologically acceptable salt, polymer, ester, or 3-hydroxy-3-methylbutanoic acid, a physiologically acceptable salt, polymer, ester, or tributyrin or tributyrin derivative, or mixture thereof.

The invention discloses an application of butyric acid or a derivative thereof in preparation of a zearalenone reproductive toxicity resisting preparation. Also disclosed is an antitoxic feed composition comprising a specific base ration and a butyric acid derivative. In-vivo and in-vitro tests and molecular mechanism researches prove that butyric acid and derivatives thereof (such as tributyrin and sodium butyrate) can effectively relieve zearalenone (ZEN)-induced animal ovary injury. The action mechanism of the traditional Chinese medicine composition is that follicular atresia is reduced, the balance of reproductive hormones (such as GnRH, E2, AMH and P4) is recovered, and clinical symptoms caused by ZEN poisoning such as vulva swelling and the like are relieved by activating a BMPs / SMAD signal channel, especially up-regulating SMAD4 protein expression and inhibiting oxidative stress (ROS) and Caspase-3 mediated granular cell apoptosis.

The invention relates to the technical field of aquaculture feeds, and particularly discloses a high-protein aquatic feed for improving meat quality of micropterus salmoides and a preparation method of the high-protein aquatic feed. The preparation method of the feed comprises the following steps: mixing the carnitine, the astaxanthin, the phytosterol, the tributyrin, the yucca extract, the meat structure-effect core premix, the monocalcium phosphate and the choline chloride to obtain a core mixture; extruding and puffing bran, mixing the extruded and puffed bran with the core mixture, and performing negative pressure treatment to obtain a feed core compound; and finally, carrying out glucose-chitosan-zinc lysine inner layer coating and sodium alginate gel outer layer encapsulation to prepare the high-protein aquatic feed. The activity of functional components is effectively protected through a three-stage protection system, targeted slow release of intestinal tracts is achieved, the bioavailability is remarkably improved, the growth performance of largemouth bass can be remarkably improved through the obtained feed, the feed coefficient and the abdominal fat rate are reduced, meanwhile, the muscle hardness, elasticity and collagen content are improved, and the meat quality is comprehensively improved.

The invention relates to the technical field of feed additives, in particular to a feed additive for preventing goose gout based on bacterium-enzyme synergistic targeted release as well as a preparation method and application of the feed additive. The invention provides a feed additive for preventing goose gout based on bacterium-enzyme synergistic targeted release, which comprises the following components in parts by weight: 5-10 parts of enveloped tributyrin and 5-10 parts of an enzyme-producing symbiotic bacterium composition, the enzyme-producing symbiotic bacterium composition comprises a fermented product obtained by carrying out symbiotic fermentation on pediococcus pentosaceus SMM914 and bacillus subtilis. In the formula of the feed additive, the slow-release characteristic of the enveloped tributyrin is utilized to ensure that the enveloped tributyrin and the enzyme-producing symbiotic bacterium compound synchronously reach the rear section of the intestinal tract, the butyric acid is efficiently utilized, targeted positioning release is realized, and the butyric acid can be continuously released to achieve a stable relieving or treating effect.

The invention provides a tributyrin solid preparation, a preparation method thereof and a feed additive containing the solid preparation, and belongs to the technical field of feed additive.The tributyrin and PLGA are dissolved in acetone and injected into a crystallization kettle through a nozzle under the supercritical CO2 condition, and a solvent is rapidly extracted to form high-encapsulation-efficiency nanometer microspheres; carrying out high-speed shearing and high-pressure homogenization treatment on the microspheres and a water phase containing maltodextrin, hydroxypropyl-beta-cyclodextrin, Arabic gum and the like to form a submicron emulsion; and finally, performing spray drying to obtain spherical microcapsule powder, and tabletting or filling capsules. The solid preparation comprises tributyrin, PLGA (poly (lactic-co-glycolic acid)), a viscoelastic matrix and a plurality of functional auxiliary materials, has the characteristics of high encapsulation efficiency, uniform particle size, good stability and low water content, and is compounded with a protein carrier, a carbohydrate carrier and the like as a core component; the prepared feed additive can effectively improve the processing stability, palatability and bioavailability of tributyrin.

The screening method comprises the following steps: S1, sample treatment: taking a high-temperature yeast sample, adding sterile water, oscillating and uniformly mixing, and then carrying out gradient dilution; s2, primary screening: coating a PDA culture medium with the diluent, and separating and purifying yeast strains through colonial morphology observation and microscopic morphology observation after constant-temperature culture; s3, secondary screening: inoculating the purified yeast strain to a tributyrin solid culture medium, performing constant-temperature culture, and screening out a strain with relatively high ester production capacity according to the size of a transparent circle around a bacterial colony; s4, molecular biological identification: constructing a clear strain taxonomic status through sequence alignment and phylogenetic tree construction; and S5, fermentation condition optimization: determining key influence factors through a single factor test, and optimizing fermentation conditions by adopting a response surface test to obtain optimal ester production fermentation parameters. According to the method, high-efficiency screening and fermentation condition optimization of the high-yield ester yeast in the high-temperature yeast for making hard liquor are realized through targeted screening and accurate identification.

The present application belongs to the technical field of feed additive, and particularly relates to a compound fatty acid preparation for promoting chicken growth, and a preparation method and application thereof. The compound fatty acid preparation for promoting chicken growth is mainly composed of the following raw materials in parts by weight: 3-6 parts of stigmasterol, 2-4 parts of campesterol, 10-20 parts of linoleic acid, 12-24 parts of tributyrin, 1-2 parts of emulsifier, 3-5 parts of soybean phospholipid, 3-5 parts of protective agent, and 30-80 parts of water. The combination of stigmasterol and campesterol can significantly improve the targeted release efficiency of linoleic acid and tributyrin. The released butyric acid can activate the mitochondrial beta-oxidation pathway of intestinal epithelial cells, significantly improve the efficiency of tricarboxylic acid cycle, and enhance the intestinal barrier function. The phytosterol can also competitively inhibit the NPC1L1 transporter, reduce the intestinal cholesterol absorption, direct the energy to the growth demand, thereby improving the daily weight gain of broilers and reducing the feed-meat ratio.

Compositions and methods for improving or regulating GLP-1 levels, or increasing GLP-1 receptors, or reducing GLP-1 degradation, or stimulating GLP-1 secretion in a subject. The composition comprises two or more of the following substances: an effective amount of dihydroberberine (DHB) or berberine, a physiologically acceptable salt, polymer, ester, acid thereof; gymnema sylvestre or gymnemic acid; cinnamon or ceylon cinnamon; nobiletin; or the composition comprises an effective amount of dihydroberberine (DHB), a physiologically acceptable salt, polymer, ester, acid, and one or more of the following substances: β-aminoisobutyric acid, a physiologically acceptable salt, ester; 3-hydroxy-3-methylbutanoic acid, a physiologically acceptable salt, ester; tetrahydrocurcumin, a physiologically acceptable salt, acid, ester; tributyrin; berberine; resveratrol; rhodiola rosea; cinnamon; fenugreek; olive leaf extract; grain of paradise; fruit-vegetable powder; whey protein; or arginine.

The invention discloses a method for accurately determining the activity of swine-derived co-lipase, which comprises the following steps: emulsifying tributyrin by using gum arabic powder and glycerol together, and performing enzymolysis on the tributyrin into free fatty acid according to the coenzyme action of co-lipase and the combined action of co-lipase and lipase, thereby obtaining the activity of the swine-derived co-lipase. Meanwhile, the blank that the tributyrin is independently enzymolyzed by the same amount of lipase is taken, finally, the activity of the co-lipase is calculated according to the calculation formula by utilizing the volume difference value of the sodium hydroxide solutions consumed for generating the free fatty acid under the two conditions, and the method for designing the activity of the pig-derived co-lipase is stable and accurate in detection result.

The invention belongs to the technical field of functional foods and health foods, and particularly relates to a composition capable of improving intestinal health, enhancing organism immunity and regulating mood health through an intestine-brain axis and application. The composition is prepared from the following components in parts by weight: 44 to 74 parts of plant extract, 7 to 25.5 parts of inactivated probiotics, 10 to 20 parts of short-chain fatty acid and 22 to 44 parts of prebiotics, the plant extract comprises at least one of an oregano extract, a cinnamon extract, a ginger extract, a turmeric extract and a spearmint extract; the short-chain fatty acid comprises at least one of tributyrin microcapsule powder, sodium butyrate and calcium butyrate. The four components are combined for use in a specific amount ratio range, can form a complementary effect on the basis of respective effects, and comprehensively act on the intestinal tract, the immune system and the intestinal-brain axis to form a collaborative network, so that the intestinal tract health is comprehensively improved, the immunity is enhanced, and the mood health is regulated.

The invention discloses a feed additive containing tributyrin and a preparation method thereof. The feed additive is prepared by the following steps: dispersing microspheres in a mixed solvent, adding tetraethoxysilane for reaction to obtain a product, adding an etching agent for stirring reaction to obtain HMSNs, dispersing the HMSNs in a solvent, adding a coupling agent for reaction to obtain aminated HMSNs, dissolving carboxymethyl chitosan and an activating agent in a buffer solution, adding a coupling agent for reaction to obtain aminated HMSNs, and performing ultrasonic dispersion to obtain the feed additive. The preparation method comprises the following steps: adding aminated HMSNs to react to obtain HMSNs modified by CMCS on the inner wall, dispersing the HMSNs modified by CMCS on the inner wall in a solvent, adding succinic anhydride to carry out ring-opening reaction, adding ethanolamine to stir to obtain a product, dispersing the product in a solvent, adding a catalyst and palmitoyl chloride to obtain HMSNs modified by CMCS on the inner wall and palmitate on the outer wall, and carrying out reaction on the HMSNs modified by CMCS and palmitate on the outer wall. And finally, mixing with an oil phase formed by mixing tributyrin and glycerol monocaprylate. The feed additive provided by the invention can improve the utilization rate and the acting effect of active ingredients.

The application provides a tributyrin solid preparation and a preparation method thereof, and a feed additive containing the solid preparation, and belongs to the technical field of feed additives.The tributyrin and PLGA are dissolved in acetone, injected into a crystallization kettle through a nozzle under the condition of supercritical CO2, and the solvent is quickly extracted to form high-encapsulation-rate nanometer microspheres; the microspheres and an aqueous phase containing maltodextrin, hydroxypropyl-beta-cyclodextrin, gum arabic and the like are subjected to high-speed shearing and high-pressure homogenization treatment to form a submicron emulsion; finally, spherical microcapsule powder is prepared through spray drying, and the powder can be tabletted or filled into capsules.The solid preparation contains tributyrin, PLGA, a viscoelastic matrix and various functional excipients, has the characteristics of high encapsulation rate, uniform particle size, good stability and low water content, and when the solid preparation is used as a core component and is compounded with a protein carrier and a carbohydrate carrier, the prepared feed additive can effectively improve the processing stability, palatability and bioavailability of the tributyrin.