222results about How to "Fully digested" patented technology

The invention relates to a kind of decellularized blood vessel matrix gel, a preparation method therefor and applications thereof. The preparation method is as follows: first, after blood vessel outer membranes and anadesma are removed, the blood vessels are cut into blood vessel pieces, the blood vessel pieces are subjected to decellularized treatment in a TritonX-100 solution, then subjected to freeze drying, smashed into powder, and filtered with a 60-mesh screen, and decellularized blood vessel matrix powder is obtained; second, the decellularized blood vessel matrix powder is placed in a hydrochloric acid solution containing pepsin with a concentration of 0.09-0.11%, and subjected to digestion for 66-78h, a sodium hydroxide solution is added to neutralize hydrochloric acid, then PBS is added to balance ion concentration, finally, the gel solution is placed in an incubator with a temperature of 37 DEG C for gel forming. The decellularized blood vessel matrix gel has good molding capability and mechanical properties, the interior is loose, the gel has certain poriness, and the collagen fiber has appropriate diameters and good stability. The gel can meet requirements of preparation of medicines treating ischemic diseases, support materials of tissue engineering and/or cell culture.

The invention relates to the field of medical jurisprudence inspection, in particular to a diatom inspection method in medical jurisprudence. The method at least comprises the following steps: (1) microwave digestion: adding concentrated nitric acid and hydrogen peroxide solution into the inspected sample to carry out microwave digestion; (2) vacuum filtration: carrying out vacuum filtration on the digestion solution, adding ultrapure water, carrying out vacuum filtration continually until the surface of the filter membrane is approximately neutral, adding absolute ethyl alcohol, and carrying out vacuum filtration to remove moisture from the filter membrane; (3) a scanning electron microscope automatically takes pictures and stores the pictures; and (4) qualitative and quantitative analysis on diatom: carrying out inspection, classification and statistic treatment on the diatom in the on-spot pictures in an artificial identification way or computer automated identification way. The invention has the advantages of high detection sensitivity, high accuracy of qualitative and quantitative analysis, simpleness, high efficiency and environmental protection, can effectively avoid pollution, greatly improves the working environment for diatom inspection technicians in medical jurisprudence, and reduces the labor intensity, thereby having wide application prospects in drowning diagnosis practice in medical jurisprudence.

The invention discloses a feed for the late-stage fattening and lactation promoting of livestock/poultry and ruminants. The feed is prepared from 80-99 parts by weight of oil and grease and 1-20 parts by weight of additives and is characterized in that the oil and grease are emulsified animal oil and/or emulsified vegetable oil, and the additives are one or more of vitamins, amino acids, enzyme preparations and mineral substances. The invention further provides three preparation processes corresponding to formulae so as to efficiently and stably obtain required feed products. According to the feed, the ingredients are scientific in proportioning ratio, balanced in nutrition and easy to digest and absorb, so that the labor intensity for farmers is reduced greatly, the effect on the fattening and lactation promoting of the livestock/poultry and ruminants at the late stage of growth is good, and the economic benefit of various feeding can be increased remarkably.

The invention provides an additive for balancing the gastrointestinal health of animals, which is prepared by absorbing 3 to 15 percent of tributyrin, 21.5 to 52.5 percent of lactic acid and 3.5 to 7.5 percent of formic acid with special carrier white black carbon by grade and layer respectively and then combining the mixture with 1 to 10 percent of glutamine. The additive has the effects of controlling diarrhea and accelerating restoration of injured intestinal tract, and starting from a philosophy of balancing the relationship between the gastropore and the intestinal tract, saving resources, reducing environment loads and improving quality of livestock products, a comprehensive gastrointestinal health management scheme is provided.

An anti-diarrhea complete formula feed for piglets comprises, by weight, 55-56 parts of extruded corn, 6-10 parts of broken rice, 13-18 parts of enzymatic soybean meal, 9.5-10 parts of low-protein whey powder containing 3% of CP, 3-5 parts of Peruvian steam fishmeal, 0.8-1 part of L-lysine hydrochloride, 0.13-0.18 parts of DL-methionine, 0.09-0.13 parts of L-tryptophan, 0.35-0.4 parts of L-threonine, 0.25-0.3 parts of L-valine, 0.1-0.15 parts of L-isoleucine, 0.18-0.2 parts of L-cystine, 0.08-0.12 parts of choline chloride, 0.83-0.88 parts of calcium hydrogen phosphate, 0.95-1 part of calcium carbonate, 0.8-0.88 parts of salt, 0.04 parts of a vitamin premix, 0.4 parts of a trace elements premix and 0.03-0.05 parts of other auxiliary additives. A feed nutrient ration is reduced, and reasonable burdening is carried out by treating an amount required by an NRC2012 piglet as a standard, so the digestible nutrient content of the feed is improved, thereby the feed resource is reduced, the nutrient draining amount of an animal is reduced, the intestinal digestion burden is reduced, and the diarrhea is mitigated.

The invention refers to a milking sow feed and a preparation method thereof, wherein the milking sow feed is prepared from yeast protein powder, hulled soybean meal, soybean meal, cottonseed meal, DDGS (distillers dried grains with solubles), rapeseed meal, pea protein powder, fish meal, blood cell protein powder, meat meal, edible soybean oil, calcium hydrophosphate, 50% choline chloride, specific complex enzyme for sow, specific premix compound for sow, complex vitamins and the like via specific processes. The milking sow feed is prepared via strict raw material selection and rational formulation proportioning so that the milking sow feed is balanced and comprehensive in nutrition, strong in pertinence, excellent in taste attractant and stability; furthermore, the milking sow feed is capable of greatly improving the milking quantity of the milking sow, and increasing the daily gain of the milking sow; and the product is low in production cost, high in efficiency, stable in quality, convenient in storage, free of adverse side effect and the like.

The invention provides a method for simultaneously determining trace gold, silver and palladium in a geochemical sample. The method comprises the following steps: firstly, digesting the geochemical sample by using 1:1 chloroazotic acid in a constant-temperature boiled water bath in a sealed manner; secondly, selectively enriching the trace gold, silver and palladium by using diphenylthiourea loaded polyurethane foam, and separating other interference elements; and finally, drawing a standard curve of Au197, Ag107 and Pd105 by adopting an inductively coupled plasma source mass spectrometer through a standard curve method, and simultaneously determining the trace gold, silver and palladium in the sample. According to the method provided by the invention, the problem that other main interference elements with the abundance of geochemical elements, which is hundreds, even thousands of times of that of the silver and the palladium, have interference on mass spectrums of the silver and the palladium is solved; and compared with a traditional emission spectrum and graphite furnace atomic absorption spectrometry, the sampling amount in analysis of the silver in the geochemical sample is increased by dozens of times, so that the influences caused by gold grain effect of the geochemical silver are effectively overcome.

The invention relates to a titanium dioxide content measurement method. The titanium dioxide content measurement method comprises the following steps: (1) digesting a polyester sample by a wet-acid soluble decomposition system; (2) reacting a dilute acid mixture of hydrochloric acid and nitric acid with non-titanium metal salt in a sample to obtain a digestion sample liquid; (3) masking metal ions, which interfere with a main reaction, by ascorbic acid, and preparing a dilute solution to be measured with the absorbance value in the range of 0.2 to 0.8 by taking hydrogen peroxide as a color developing agent; (4) measuring the absorbance value A of the dilute solution to be measured, and measuring the blank absorbance value A0; (5) preparing standard solutions of titanium with a series of ladder concentration, measuring the absorbance values of the standard solutions, and then drawing a standard working curve Y=KX by taking the difference values of the measured absorbance values and the blank absorbance value as horizontal coordinates X and taking the titanium content corresponding to the standard solutions of titanium with a series of ladder concentration as vertical coordinates Y, thereby obtaining the slope value K of the standard working curve; and (6) calculating the percentage of titanium dioxide TiO2. By the measurement method disclosed by the invention, the content of titanium dioxide in polyester can be measured accurately.

The invention provides a sample pretreatment method simultaneously determining arsenic and mercury in milk and dairy products. The method comprises weighing 0.3-1.0 g of to-be measured milk or dairy product in a digestion tank, adding 2.0 mL of hydrogen peroxide and 4.0 mL of nitric acid, so as to obtain a to-be digested solution, wherein the mass fraction of hydrogen peroxide is 30%, and the mass fraction of nitric acid is 65.0%-68.0%; performing microwave digestion on the to-be digested solution; cooling the solution obtained after microwave digestion, adding 1.0 mL of a saturated sodium hydroxide solution, so as to obtain a neutralization solution; and transferring the neutralization solution to another container by using hydrochloric acid, then adding 2.5 mL of a thiourea solution with the concentration of 50 g/L, finally using a hydrochloric acid solution to set a constant volume of 25 mL, and uniformly mixing the solution. The process does not need acid expelling, the sample can be completely digested, and loss of arsenic and mercury elements is relatively small. By using the sample pretreatment method for detection, the detection result is relatively accurate.

The invention provides a method for separating and culturing mouse cells, comprising the following steps: (1) disinfecting tissue processing instruments; (2) selecting male BALB/c mice aged 1 to 2 months, killing them by dislocation of the neck, and using surgical scissors Separate the synovial layer and fibrous layer of the joint capsule, then take out the synovial layer tissue, put the tissue into pre-cooled sterile double-antibody-containing PBS solution and wash several times to remove blood stains and fat tissue; (3) Cut the tissue into pieces and use Digest with preheated type II collagenase and trypsin respectively; (4) terminate the digestion, centrifuge, and discard the supernatant; (5) inoculate into the coated culture flask by artificial adherence method; (6) after incubation for 1-2 hours, Cultured in an environment of 37°C and 5% CO2; (7) observation of cell morphology; (8) subculture of cells; (9) identification of cells. The method for isolating and culturing mouse synoviocytes provided by the invention is simple to operate, has a large number of cells and a high survival rate, and is an ideal primary method for isolating and culturing mouse synoviocytes, providing a reliable basis for experiments. cellular resources.

The invention provides a cattle breeding method, and belongs to the technical field of cattle breeding. The breeding method comprises the following steps that firstly, a cattle breeding farm is built; secondly, cattle in different growth periods are fed on a mixture of a coarse material and a fine material in corresponding proportion, wherein the coarse material is prepared from straw, green grass and wheat bran, and the fine material is prepared from corn, soybean meal, rape seed cakes, cottonseed cakes, animal bone meal, malt, lotus petioles, lotus seeds, tangerine pith, pericarpium citri reticulatae, cortex magnoliae officinalis, baking soda, vegetable oil and edible salt; lastly, parasite expelling is conducted for the cattle regularly. According to the breeding method, the adopted feed is prepared from the natural raw materials through scientific combination and is rich in bioactive substances, effective ingredients of all the raw materials are mutually influenced and achieve a synergistic effect, therefore, the immunity of the cattle can be improved, the disease incidence can be decreased, and the quality and the edible safety of beef can be improved.

The invention discloses a regenerative foam material taking granite powder as a raw material and a preparation method of the regenerative foam material. A formula of the regenerative foam material comprises the following components in parts by weight: 40-69 parts of granite powder, 2-8 parts of fly ash, 6-25 parts of fluxing filler, 1-5 parts of pore forming material, and 1-5 parts of polymeric material. The preparation method of the regenerative foam material sequentially comprises the following steps that grain fineness of the powder is controlled above 100 meshes; the powder is stirred and mixed uniformly; a mixture is pressed to a required granular material; the granular material is sent to a high-temperature furnace for warming, cooled and taken out; and a finished product is obtained. The regenerative foam material and the preparation method have the advantages that granite powder waste can be digested sufficiently; a long-term severe environmental pollution problem of a granite processing base can be solved; and environmental requirements can be met. The regenerative foam material is stable in physical property, light in weight, heat-insulated, compression-resistant and fracture-resistant, and can be used for building wall or external wall decoration; objectives of building weight reduction and energy saving can be achieved; in addition, the regenerative foam material has fireproof, waterproof and soundproof properties, and can be used for fireproof materials and wall partitioning materials.

The invention discloses a calcium hydroxide continuation production process and a dedicated production line, the calcium hydroxide continuation production process belongs to a calcium hydroxide production process, and mainly aims to solve complex process equipment, high production costs, low efficiency and other technical issues in the prior art. The technical program elements are that: the dedicated production line includes a material distribution device, digestion tanks and a sorting machine, the material distribution device is connected via a transfer mechanism with an inlet of a digestion device, the digestion device is at least provided with two digestion tanks which are connected in turn, an outlet and an inlet of adjacent digestion tanks are connected in turn, a sorting machine feed inlet and the digestion tank outlet are communicated via a transfer mechanism, sorting machine outlets comprise a powder outlet and a slag outlet, a powder separator wind outlet is connected with a material storage tank, and is communicated with a finished product output mechanism, and a closed loop is formed by mutual seal connection. The dedicated production line can prevents the environmental pollution caused by accidental leaks during production, is energy-saving and emission-reducing, and is mainly used in the field of production of calcium hydroxide.

The invention relates to a detection method and in particular relates to a method for detecting organic carbon in plants and soil and a heating device of the method. The method for detecting organic carbon in plants and soil comprises the following steps: pretreating a plant sample or a soil sample, mixing the pretreated plant sample or the pretreated soil sample, potassium dichromate solution and concentrated sulfuric acid solution in a triangular flask, heating to carry out oxidizing reaction, adding distilled water after the reaction is ended, cooling and adding phenanthroline solution as an indicator, titrating the residual potassium dichromate in the oxidizing reaction by using ferrous sulfate solution, and calculating the content of organic carbon, wherein the oxidizing reaction refers to reaction under the condition of normal pressure steam generated by water at the temperature of 90-100 DEG C for 20-60 minutes. The normal pressure steam heating method is simple to operate and easy to control, the temperature in a water bath kettle is stable and easy to control, the sample is uniformly heated and fully digested, and the method is safe, environmentally friendly, high in detection reproducibility, accurate and efficient.

The invention discloses a sensitive, reliable, rapid and stable synchronous detection method for iron ore multi-elements, and belongs to the technical field of test and analysis of metallurgy materials. The method comprises the following steps: adding 0.1-0.2g of iron ore powder to 200-250ml/g mixed acid of hydrochloric acid and nitric acid with the ratio of (3-5):1 to dissolve; adding 7-12ml/ml hydrofluoric acid, and heating at 200-250 DEG C for 10-15 minutes; adding 4-6ml/ml perchloric acid, heating at 200-250 DEG C until the residual solution is 0.8-1.2ml; adding 10-15ml/ml diluted hydrochloric acid; and filtering after dissolving, and diluting into 0.0008-0.0012g/ml to obtain a solution to be detected. Standard solutions of the elements with different concentration gradients are prepared, an analytical spectral line and a working condition of each element in an inductively coupled plasma emission spectrum are selected, the emission intensity of the analytical spectral line of each element is determined to draw a standard curve, the emission intensity corresponding to each element is detected, and the content of each element is determined according to the standard curve. The method is sensitive, reliable, rapid and stable, analysis data can be timely provided for the smelting production process, the production efficiency and the utilization ratio of equipment are improved, and the application promotion value is relatively high.

The invention belongs to the microbial fermentation field, and particularly relates to a fermentation method of soybean meal, which adopts a formula that: the ratio of soybean meal to sweet potato(corn) starch: salt: yeast: active 99 fermenter: coarse fodder degrader: calcium hydrophosphate: lysine: threonine: ferrous sulfate: zinc oxide is 100: 2 to 10: 0.2 to 0.4: 0.025: 0.036 to 0.04: 0.036 to 0.04: 0.4 to 0.6: 0.1 to 0.2: 0.07 to 0.1: 0.01: 0.001. The raw materials are placed into a blender to be mixed with 1000 to 1200kg of water, then the mixture enters an underground fermentation pool, and the underground fermentation pool is covered and sealed by a film. The temperature is maintained within the temperature range of 15 to 35 DEG C through the terrestrial heat, and after being fermented for 5 to 10 days, the soybean meal can be directly used for feeding animals. The fermentation method of the soybean meal is simple to operate, protein inside the soybean meal can be thoroughly decomposed, and contents of the lysine, threonine and trace elements of iron and zinc are high and are easy to assimilate by the animals.

The invention relates to a suspension chip for detecting shigella and a detecting method thereof. The suspension chip comprises a microsphere carrier and an oligonucleotide probe fixed on the carrier, wherein the oligonucleotide probe is a fragment of DNA sequence in specific gene invasive plasmid antigen H screened from the shigella. The detecting method comprises the following steps: after a genome DNA in a sample to be detected is amplified and labeled by a designed primer, using the suspension chip to hybridize the genome DNA, and judging whether the sample to be detected contains the shigella according to hybridized fluorescence intensity. The suspension chip has high detection sensitivity which can reach a level of 1.18fg genome DNA (1fg corresponds to 2 numbers of bacteria), so the method can fully meet detection requirements of clinical samples and environmental samples, has the characteristics of high specificity, simple operation, low cost and the like, and is easy to popularize. And the method adopts a PCR reaction system of UNG-Taq enzyme to reduce PCR false positive results greatly.

The invention relates to a sintering quicklime digestion device and method. The device comprises a raw material belt, a ditch scraping plate, a material flatting plate, a quicklime disc distributor and a discharge belt. At least one material distributing plate is inclinedly disposed between the discharge belt and the raw material belt, over two material distributing plates can adopt a layering setting form, and a digestion water pipe is disposed below each material distributing plate. The ditch scraping plate is employed to perform ditch scraping on raw materials, the quicklime disc distributor and the discharge belt are adopted for filling, layer-by-layer digestion is carried out according to a digestion water flow of 950-1000ml per kilogram of quicklime, and the material flatting plate is utilized to conduct material flatting and burying. According to the invention, by setting the quicklime shunt on the basis of an original sintering quicklime digestion device, layered group digestion of quicklime can be realized, so that quicklime digestion can be more complete, CaO in the raw materials can be distributed more uniformly, the sintering mixing granulating effect can be better, the sinter feed greater than 3mm particle fraction is increased by 5%, and the mixture temperature is raised from 62DEG C to 75DEG C.

An efficient dry-type anaerobic fermentation equipment for organic solid waste is disclosed. A horizontal main tank is provided with a material inlet, a slag outlet, a gas outlet and a liquid outlet; the main tank is inside divided into multiple fermentation chambers by separation plates, a stirrer is horizontally inserted along the center of the main tank, the bottom of the main tank is provided with a biogas discharging device which is communicated with the slag outlet, and the bottom of each separation plate is provided with a communicating pore. The efficient dry-type anaerobic fermentation equipment is high in organic loading, high in gas production efficiency, small in land occupation area and low in energy consumption, and also helps to avoid problems of biogas residue crusting and secondary pollution existed in wet-type anaerobic fermentation.

The invention provides a separating and culturing method for olfactory ensheathing cells of rats. The separating and culturing method comprises the following steps: (1) disinfecting a tissue processing instrument; (2) selecting male SD rats of 2-3 months old, killing the male SD rats after anesthesia, separating the olfactory mucosa tissue by adopting surgical scissors, placing the tissue in PBSsolution which is precooled, is sterile and contains double antibodies, and washing the tissue for a plurality of times, so that blood stains and outer membranes are removed; (3) cutting up the tissue, and carrying out digestion by adopting preheated neutral enzyme and trypsin respectively; (4) terminating the digestion, carrying out centrifugation, and discarding the supernatant; (5) inoculatingthe obtained product into an enveloped culture flask by adopting an artificial wall sticking method; (6) carrying out incubation for 1-2 h, and carrying out culturing in the environment being at 37 DEG C containing 5% CO2; (7) carrying out cell purification; (8) carrying out cell morphological observation; (9) carrying out cell subculturing; (10) carrying out cell counting and survival rate testing; and (11) carrying out immunological identification. For the separating and culturing method for olfactory ensheathing cells of rats, the operation is simple, a large number of cells are obtained, the survival rate is high, the method belong to an ideal primary separating and culturing method for the olfactory ensheathing cells of rats, and a reliable cell resource is provided for the experiment.