344 results about "Digestion Treatment" patented technology

An apparatus for the anaerobic digestion of solid waste is disclosed. The apparatus includes a pre-digestion treatment chamber and an anaerobic digester chamber coupled to the pre-digestion treatment chamber. A liquid suspension of solid waste is pretreated under aerobic conditions by heating to a desired pre-digestion temperature. One or more chemical or biological additives are added to the suspension of solid waste to improve the anaerobic digestibility of the solid waste. This may include enzymes to destruct poorly digestible solids and / or materials to provide a desired C:N ratio in the solid waste. The apparatus may include mixers, heaters, chemical or biological additives as needed, various sensors and probes, and a control system to monitor and control the anaerobic digestion process and maintain microbial health of the digester.

The invention discloses a method for eliminating mecA plasmids based on CRISPR / Cas9 technology. The method comprises: selecting an MRSA strain to perform PCR amplification to a DNA sequence of a C terminal of mecA gene coding transpeptidase; carrying out gel extraction for mecA genes; connecting the mecA genes with a T-pMD19 (simple) carrier and preparing DH5[alpha] competent cells; electro-transforming the DH5[alpha] competent cells through the obtained T-pMD19-mecA plasmids; extracting the T-pMD19-mecA plasmids and performing sequencing and verification; performing double digestion treatment for the T-pMD19-mecA plasmids and performing gel extraction to the mecA genes; connecting pET-21a (+) plasmids with the mecA genes; designing and synthesizing oligos; constructing pCas9 :: mecA plasmids; electro-transforming the obtained pET-21a (+)-mecA plasmids into an escherichia coli expression strain BL21 (D3); and electro-transforming the pCas9 :: mecA plasmids and the pCas9 plasmids into BL21 (D3)+pET-21a(+)-mecA competent bacteria and BL21 (D3)+pET-21a(+) competent bacteria. The method is simple to operate and good in specificity, and can effectively block spread of mecA to eliminating the mecA strain.

The invention relates to a preparation method of aragonite crystal form calcium carbonate, comprising the following steps: lime is sent into a digestion equipment and added with water for digestion treatment, water and quick lime are added slowly according to the ratio of 3:1 to 5:1, calcium hydroxide emulsion is obtained by filtrating, the calcium hydroxide emulsion is sent into a carbonization equipment, magnesium hydroxide needle crystal is added as a seed crystal, CO2 is injected in the materials which are mixed at the same time, aragonite crystal form light calcium carbonate is obtained by carbonization reaction; the light calcium carbonate screened pulp is sent into a filtration device for dehydrating and calcium carbonate filter cake is obtained; the sodium polyacrylate is added as a dispersant and light calcium carbonate with particle size distribution of 1-2 mum is obtained after grinding, thus obtaining a dispersion liquid product which reachs 70% of solid content or the liquid product is sent into a drying device for drying to obtain light calcium carbonate dry powder. The preparation method enjoys cheap price, simple preparation process and perfect performance, thus being applicable to the industrialized production. The obtained aragonite crystal form calcium carbonate is suitable to be a papermaking coating.

The invention relates to a pretreatment method of Acesulfame-K, comprising the following steps: firstly conducting nitric acid digestion treatment, then conducting high-temperature ashing treatment, and finally dissolving. The pretreatment method disclosed herein is an improvement based on orginal dry ashing and wet ashing, simplified the operation process, and effectively reduces the possibility of sample contamination. The invention further provides a detection method for detecting potassium in Acesulfame-K after pretreating the Acesulfame-K with the above pretreatment method, characterized by pretreating an Acesulfame-K sample with the above pretreatment method and then conducting instrument detection of potassium, thus the precision of the detection can be raised, and the content of potassium in the original sample can be accurately reflected.

The invention discloses a technique for producing cold-bonded pellets from steel converter dust removal ash, which comprises the following steps: separately collecting rough dust removal ash and fine dust removal ash of the steel converter, weighing iron scale, rough dust removal ash and fine dust removal ash in a mass ratio of (10-50):(20-80):(20-70), and uniformly mixing; adding the mixture into a magnesium chloride solution, and uniformly mixing, wherein the magnesium chloride solution accounts for 1-20 wt% of the total mass; and pelletizing with a pelletizer, stripping, and naturally solidifying to obtain the cold-bonded pellets. The dust removal ash does not need previous digestion treatment with water, thereby saving abundant water resources; the production technique is simple and feasible, and is implemented only by proportionally adding the inorganic weakly-alkaline binder into the iron scale, rough dust removal ash and fine dust removal, uniformly mixing and pelletizing; by using the inorganic weakly-alkaline binder, the finished cold-bonded pellets have the advantages of fewer impurities, low SiO2 content and high total iron content (not lower than 60%); and abundant cold-bonded pellets can be used in the steelmaking process, and the steelmaking process is not influenced by the addition amount.

The invention provides an urban sewage dephosphorization denitrification treatment method, relating to a sewage treatment technology. The invention discloses a high efficiency low energy consumption urban sewage dephosphorization denitrification treatment method. The treatment method includes the following steps: urban sewage after pre-treatment and digested sludge refluxed by an anaerobic sludge digestion unit enter into an anaerobic reactor, and biochemical reaction is taken place; effluent of the anaerobic reactor, mixed liquid refluxed by an aerobic reactor and returned sludge enter into an anoxic reactor, and biochemical reaction such as denitrification is taken place; one part of effluent of the anoxic reactor enters into the aerobic reactor, and the other part returns to the anaerobic reactor; mixed liquid of effluent of the aerobic reactor enters into a secondary sedimentation tank, and mud and water are separated; and residual activated sludge is concentrated and then discharged to the anaerobic sludge digestion unit to be digested. The anaerobic sludge digestion unit digests sludge under anaerobic condition, one part of digested sludge returns to the anaerobic reactor or anoxic reactor or aerobic reactor, and the other part of digested sludge is dehydrated, and then sludge treatment is carried out.

The invention provides a biological organic fertilizer and a production technology of the biological organic fertilizer. The biological organic fertilizer comprises the following raw materials in parts by weight: 35-45 parts of vinasse, 25-35 parts of chicken manure, 5-16 parts of mushroom dreg, 7-15 parts of rice bran powder, 7-15 parts of straw and 0.01-0.03 part of a composite microorganism inoculant. The vinasse adopted by the biological organic fertilizer is small red sorghum 0 sauced vinasse, contains higher crude fat, crude fiber, crude protein and amino acid, is easy to corrupt, can accelerate rapid reproduction and digestion treatment of microorganisms, can shorten the production time of the organic fertilizer, and can increase microorganism culture content of the organic fertilizer; and the produced organic fertilizer can realize the characteristics of high crop yield, high nutrient substance content and high disease and insect resistance by selection of culture variety and quantity and improvement of the production technology.

The invention relates to methods for separating, freezing and thawing a whole cell of an umbilical cord and separating and augmenting a thawed stem cell. The method for separating and freezing the whole cell of the umbilical cord comprises the following steps of: sterilizing and cleaning an umbilical cord tissue; shearing the tissue into a block shape and carrying out digestion treatment for 1.5 hours; preparing a umbilical cord tissue frozen solution for later use; and adding the whole cell and frozen solution which are obtained by sterilizing treatment into a freezing tube, refrigerating the freezing tube for 0.5 hour at low temperature of 4DEG C, freezing the freezing tube for one day under the temperature condition of -80DEG C and then freezing the freezing tube in liquid nitrogen for later use. The method for thawing the whole cell of the umbilical cord comprises the following steps of: when the whole cell of the umbilical cord is required, extracting the whole cell of the umbilical cord from the liquid nitrogen, thawing the whole cell of the umbilical cord in a constant-temperature water bath; cleaning the whole cell of the umbilical cord by using a mesenchymal stem cell culture medium and a drop method; and augmenting a mesenchymal stem cell by using the thawed whole cell of the umbilical cord through cell culture and cell passage. According to the methods disclosed by the invention, the frozen umbilical cord tissue can be effectively protected and is convenient for thawing; and the method is especially suitable for separating and augmenting the mesenchymal stem cell after the frozen umbilical cord tissue is thawed.

The invention provides a dura graft and a preparation method thereof. The dura graft is prepared from an acellular dermal matrix. The acellular dermal matrix is obtained from allogenic skin by crosslinking treatment, digestion treatment with a protease solution, decellularization with a hypertonic saline solution containing a surfactant, DNA degradation, amino acid nutrition treatment and freeze drying. The invention also provides applications of the dura graft in preparing repair materials used in the treatment of dural damage. The acellular dermal matrix is obtained from human skin tissue and undergoes physicochemical and biochemical treatment, so that all the components that may cause immunological rejection are removed. A fibrous stereoscopic scaffold structure of the original tissue is completely reserved, and new vessels and fibroblasts rapidly grow in the dura graft after implantation. Therefore, the dura graft has good clinical effects.

The invention relates to a high-concentration anaerobic sludge digestion treatment process for carrying out mechanical dewatering on sludge, comprising the following steps of: conveying sludge with high water content into a concentration tank, wherein the water content of the concentrated sludge is reduced to 97 percent, and a supernate reflexes to a sludge treatment system; carrying out mechanical pre-dewatering on the concentrated sludge, wherein the water content is about 88-90 percent after the mechanical pre-dewatering; then carrying out anaerobic digestion on the pre-dewatered sludge under the high concentration condition by adopting a CSTR (Continuous Stirred-Tank Reactor); and further dewatering the sludge after anaerobic digestion, wherein the water content of a treated sludge cake is about 75 percent. The invention has following advantages and positive effects: by adopting the technical scheme, the dewatering effect of the sludge is favorable, the volume of an anaerobic sludge digestion tank is greatly shortened, and the capital construction investment and the operation cost of a sewage treatment plant are reduced; the load of organic matters in the sludge can be effectively improved by carrying out pre-dewatering on the sludge; and the CSTR anaerobic sludge digestion which is suitable for the high-concentration solid materials is used for treatment, which is beneficial to sludge resource utilization.

The invention provides a comprehensive treatment technology for municipal domestic sludge. The comprehensive treatment technology comprises the following steps: (1), the municipal domestic sludge is subjected to anaerobic fermentation and digestion treatment; and (2), residual sludge after anaerobic fermentation and digestion treatment is subjected to high-temperature aerobic fermentation. After two treatment processes, the municipal domestic sludge is thoroughly decomposed, finally changed to nutrient soil containing high organic matters and total NPK (nitrogen, phosphorus and potassium) nutrients, and can be used for producing various organic fertilizer products through processing. By means of the comprehensive treatment technology, not only can the municipal domestic sludge be thoroughly reduced and harmless, but also a series of environmental pollution for the air, the earth's surface, the water body and the like due to the municipal domestic sludge can be eliminated completely, the resource utilization rate of the municipal domestic sludge is increased, biogas can be produced and prepared at the anaerobic fermentation stage, sludge decomposed nutrient soil can be used for processing various organic fertilizers for landscaping, agriculture and the like at the high-temperature aerobic fermentation stage, the soil organic matters are increased, and the soil structure is improved.

The invention relates to a sludge pyrohydrolysis method and a sludge pyrohydrolysis system, belongs to the field of treatment of a sewage plant, and particularly relates to the field of treatment of sludge of a sewage treatment product. To solve the problem that most of organic matters are not removed with an existing method, the invention provides the sludge pyrohydrolysis method and the sludge pyrohydrolysis system. The method comprises the processes of sludge slurrying, sludge pyrohydrolysis, sludge decompressed flash, sludge digestion, and centrifugal dewatering, wherein the process of sludge slurrying adopts a pump stirring mode, the temperature in a slurrying tank is kept at 97 DEG C; saturated steam heats a pyrohydrolysis tank to rapidly rise the temperature to be 165-190 DEG C in the process of sludge pyrohydrolysis, the pyrohydrolysis process lasts for 90 min, wherein the time for sludge charge is 15 min, the time for sludge discharge is 15 min, the steam is introduced for about 15 min, the pyrohydrolysis reaction is about 30 min, and the air is exhausted for 15 min; in the flash process, the pressure of a flash tank is kept at 0.1-0.3 MPa and the balance temperature is 102 DEG C; the time for sludge digestion is 15 days. The method and the system use the modes of pyrohydrolysis and flash, the organic matters in the sludge are effectively removed, meanwhile, the time for sludge digestion is shortened, and the sludge treatment efficiency is improved.

The invention relates to an urban sewage dephosphorization treatment method, in particular to a sewage treatment technology. The invention discloses a zero-energy consumption urban sewage dephosphorization treatment method, which comprises the following steps: leading urban sewage after pretreatment and returned digested sludge to enter into an anaerobic tank together for carrying out biochemical reaction; leading effluent from the anaerobic tank to enter into an aerobic aeration tank to carry out degradation of organic matters and reaction of phosphorus uptake of phosphorus-accumulating bacteria; leading mixed liquor of the effluent from the aerobic aeration tank to enter into a secondary sedimentation tank for carrying out sludge-water separation; carrying out sludge concentration on the remaining activated sludge, and then discharging the concentrated sludge to an anaerobic sludge digestion unit for carrying out digestion treatment; and leading the anaerobic sludge digestion unit to digest the sludge under anaerobic conditions, leading one part of the digested sludge to return to the anaerobic tank or the aerobic aeration tank, or leading one part of the digester sludge to return to an anoxic tank and the aerobic aeration tank respectively according to proportion, and leading the other part of the digested sludge to carry out sludge disposal after dehydration.

The invention discloses a method for extracting phosphate concentrate from phosphate tailings and cooperatively producing calcium carbonate and magnesium oxide. First, phosphate tailings are calcined under high temperature, hot water is added into the calcined material for digestion treatment, an ammonium nitrate solution is added to the calcined material, the mixture is stirred, calcium is extracted under a certain temperature, calcium-containing leaching liquid and leaching residues are obtained, phosphate concentrate and magnesium-containing leaching liquid are obtained after magnesium is extracted from the leaching residues with an ammonium sulfate solution, calcium carbonate is obtained after the calcium-containing leaching liquid is precipitated by an ammonium carbonate solution, magnesium carbonate is obtained after the magnesium-containing leaching liquid is precipitated by an ammonium carbonate solution, and magnesium oxide is obtained after the magnesium carbonate is calcined. According to the method, phosphate concentrate, calcium carbonate and magnesium oxide are prepared with phosphate tailings as the main raw materials, large-scale treatment can be carried out on the phosphate tailings, the problem that because phosphate tailings pile up in quantity, the phosphate tailings occupy a lot of land and pollute the environment is solved, and the products of phosphate concentrate, calcium carbonate and magnesium oxide are obtained.

A technology for reducing and recycling treatment of sludge of a sewage treatment plant comprises steps as follows: sewage is treated with an air-floatation method, pretreated sewage and primary sludge are produced, the pretreated sewage is drained after biochemical treatment, treatment by a secondary sedimentation tank and disinfection treatment, excess sludge is separated in the secondary sedimentation tank, the primary sludge and the excess sludge are concentrated, and concentrated sludge and a supernate are separated out; the concentrated sludge has an anaerobic digestion reaction, digested sludge is subjected to incineration or landfill treatment after dehydration and drying treatment, a filtrate is produced during dehydration of the digested sludge, and biogas is subjected to power generation and / or heat recovery treatment after dehydration and desulfurization treatment; the supernate and the filtrate are mixed for nitrogen and phosphorus removal. With the adoption of the technology, the primary sedimentation effect of the sewage is improved, the yield of the primary sludge is increased, the primary sludge is removed before biochemical treatment, the yield of the excess sludge is decreased from the source, the primary sludge and the excess sludge are subjected to anaerobic digestion treatment, the higher organic matter transformation rate and the higher biogas yield can be obtained, and the sludge can be reduced and recycled.

The invention provides an in vitro culture method of cancer type organs. According to the embodiment of the invention, the method comprises the steps of performing shearing and digestion treatment oncancer tissue samples, wherein the size of cancer tissue after the shearing treatment is 1-2mm<3>, and the digestion treatment is performed under the action of combined digestive enzymes; and performing re-suspension treatment on cancer cells obtained after digestion treatment with precooling substrate glue, wherein the concentration of the cancer cells in the substrate glue is 500-1500 / 10 [mu]L;and performing point and pallet treatment on cancer cell suspension, and performing culture treatment on the cancer cells after point and pallet treatment is performed, so as to obtain cancer organs.According to the method of the embodiment of the invention, the enzymolysis efficiency of the cancer cells is improved, the success rate of subculturing of organoids is increased, and long-term culture of the organoids can be realized, and a biology sample database can be established.

The present invention relates to a livestock excrement treatment method, and particularly relates to an excrement biological treatment method for a livestock and poultry breeding farm. The method comprises the steps of adjusting the water content of the collected livestock excrement to be within the range of 50-90% to form an adjustment material; putting the larvae of black soldier flies into the adjustment material; feeding the larvae of black soldier flies on the livestock excrement, wherein the larvae of black soldier flies digest and absorb organic substances in the excrement to grow up and excrete during the growing process so as to complete the digestion treatment of the excrement during the growing and moving process, and the excrement of black soldier flies and the digested residue of the livestock excrement together form a granular organic fertilizer, namely insect sands; separating the larvae of black soldier flies from the insect sands, and machining the larvae of black soldier flies into high-value protein powders. According to the technical scheme of the invention, the organic substances of the livestock excrement are converted into insect protein powders and a high-quality organic fertilizer, and the conversion rate of the organic substances is up to be over 90%. In this way, the problem in the prior art that the livestock excrement of the livestock and poultry breeding farm pollutes the surrounding environment can be effectively solved. Meanwhile, the treatment method is simple, practical and high in efficiency.

The invention relates to a sewage treatment method, relating to a sewage treatment technology. The invention discloses a municipal sewage treatment method with zero energy consumption low sludge yield, which comprises the following steps of: leading pretreated municipal sewage into a primary sedimentation basin, and leading the outlet water of the primary sedimentation basin and active backflow sludge of a secondary sedimentation basin into an aeration basin together to form a mixed liquid; leading the mixed liquid of outlet water from the aeration basin into the secondary sedimentation basin, and leading the sedimented and clarified sewage to tertiary sewage treatment or discharging as treatment water; and concentrating the residual active sludge, and discharging the concentrated sludge to an anaerobic sludge digestion unit for digestion treatment. The anaerobic sludge digestion unit digests the sludge under anaerobic conditions, and one part of digested sludge flows back to the aeration basin or the primary sedimentation basin or flows back to the aeration basin and the primary sedimentation basin proportionally; the other part of digested sludge is treated after dehydration. The energy of methane gas is recycled so as to provide energy (electric energy and thermal energy) for sewage and sludge treatment.

The invention provides a resource treatment method of urban sewage and relates to a sewage treatment technology. The invention discloses a zero-energy-consumption enhanced AB (adsorption-biodegradation) treatment method for urban sewage, which includes the following steps of: simultaneously introducing pretreated urban sewage and digested sludge returned from an anaerobic sludge digestion unit into an adsorption tank for biochemical adsorption reaction; introducing the effluent of the adsorption tank into an intermediate settling tank for sludge-water separation and introducing clarified sewage into an aeration tank; carrying out organic matter biodegradation, nitrification and other biochemical reactions on the sewage and sludge in the aeration tank; introducing the effluent mixed liquidof the aeration tank into a secondary settling tank for sludge-water separation; concentrating the excess sludge discharged from the intermediate settling tank and secondary settling tank sludge and discharging to the anaerobic sludge digestion unit for digestion treatment; and digesting the sludge in the anaerobic sludge digestion unit under an anaerobic condition, returning part of the digestedsludge to the adsorption tank or the aeration tank and dewatering the other part of the digested sludge for sludge treatment.

The invention discloses a clean production method for preparing xylitol by using agricultural and forestry waste. The method comprises the following steps of: performing crushing, dust removal and washing pretreatment on an agricultural and forestry waste raw material; soaking the obtained agricultural and forestry waste granules; performing high-temperature digestion treatment on the treated agricultural and forestry waste granules; performing neutralization or over-neutralization treatment on the treated system; performing solid-liquid separation on the treated system, and performing decolorization and detoxification treatment on the obtained liquid components by using active carbon or ion exchange resin; concentrating the treated liquid components to obtain high-concentration digestion liquid for xylitol fermentation; screening and breading high-yield strains for producing the xylitol by substrate fermentation; and producing the xylitol by fermentation. The method is a novel clean process for producing the xylitol, has simple process and easy operation, and is favorable for purification and quality control of a product.

A bionic digestion determination method for the total quantity of digestible carbohydrates of feed includes the following steps that 1, sample preparation is carried out; 2, a buffer solution and simulated digestive juice of the stomach and the small intestine and other reagents are prepared; 3, a monogastric animal bionic digestive system is preheated; 4, sample loading is carried out; 5, stomach simulated digestion treatment is carried out; 6, small intestine simulated digestion treatment is carried out, wherein small intestine simulated digestion parameters are set through monogastric animal bionic digestive system control software, and small intestine simulated digestion treatment is started; 7, digestion residue treatment is carried out; 8, the content of reducing sugar in a control sample and the content of reducing sugar in an analysis sample are determined with the DNS colorimetric method, and the total quantity of digestible carbohydrates of the feed is worked out. According to the bionic digestion determination method for the total quantity of digestible carbohydrates of the feed, the external digestion process is automatically controlled in the monogastric animal bionic digestive system, and the test result is high in reproducibility and precision; the workload of operators is reduced, system errors caused by manual operation are eliminated, and the detection efficiency is high.

The invention discloses a compound microbial agent for anaerobic digestion treatment of sludge and a production method of the compound microbial agent. The compound microbial agent is a liquid microbial agent which is prepared through fermentation culture and comprises the following bacteria: bacillus subtilis, streptomyces albus, streptomyces griseus, candida utilis, aspergillus sojae, lactobacillus bulgaricus, lactococcus lactis, rhodopseudomonas palustris, rhodopseudomonas capsulate and rhodobacter sphaeroides, and the number of total viable bacteria in 1ml of the compound microbial agent is not less than 2*10<8>. All the bacteria used by the compound microbial agent have metabiosis and symbiosis effects and form a biodegradation system with very high enzymatic activities. When the compound microbial agent disclosed by the invention is used for carrying out anaerobic digestion treatment on sludge, the degradation rate of organic matters can be effectively increased, the sludge hydrolytic process can be accelerated, the anaerobic digestion time of the sludge can be shortened, and the biological gas yield can be increased.

The invention relates to a method for the combined test of total nitrogen and total phosphor phosphorus in soil, which comprises the following steps: (1) synchronous digestion treatment of soil: weighing a dried and screen soil sample, placing the sample into a digestion tube, adding an accelerator, adding solution of concentrated sulfuric acid and placing the digestion tube in at high-temperatureenvironment for digestion; (2) preparation of soil liquid to be tested: after the digestion liquid obtained after digestion is cool, fixing the volume of the digestion liquid by adding distilled water, using the digestion liquid to test the total nitrogen content of the soil, adjusting the pH value of soil total nitrogen liquid to be tested by using alkaline liquid and using the resulting liquidto test the total phosphor content of the soil; and (3) test of the total nitrogen content and total phosphor content of soil: automatically testing the total nitrogen content and total phosphor content of soil by using a constant flow injection analyzer. The method has the advantages of: 1, avoiding repeated soil sample weighing and soil digestion and simplifying experimental operation process; 2, avoiding damages of selenium powder to human bodies by using potassium sulfate and copper sulfate in place of the accelerator for soil digestion treatment; and 3, improving accuracy and precision bytesting total nitrogen content and total phosphor content of soil by using constant flow injection analyzer.

The invention discloses a method for extracting phosphate concentrate from phosphate tailings and cooperatively producing calcium ammonium nitrate and magnesium ammonium sulphate. Phosphate tailings are calcined under high temperature, hot water is added into the calcined material for digestion treatment, an ammonium nitrate solution is added to the calcined material, the mixture is stirred, calcium is extracted under a certain temperature, calcium-containing leaching liquid and leaching residues are obtained, phosphate concentrate and magnesium-containing leaching liquid are obtained after magnesium is extracted from the leaching residues with an ammonium sulfate solution, and calcium ammonium nitrate and magnesium ammonium sulphate are obtained after the calcium-containing leaching liquid and the magnesium-containing leaching liquid are concentrated and dried. According to the method, phosphate concentrate, calcium ammonium nitrate and magnesium ammonium sulphate are prepared with phosphate tailings as the main raw materials, large-scale treatment can be carried out on the phosphate tailings, the problem that because phosphate tailings pile up in quantity, the phosphate tailings occupy a lot of land and pollute the environment is solved, and the obtained phosphate concentrate, calcium ammonium nitrate and magnesium ammonium sulphate can bring certain economic interests.

The invention discloses a processing method of fruity black tea condensed juice, and belongs to the technical field of black tea beverage processing methods. The processing method comprises the following processing steps of (1) deterioration treatment; (2) fruit and deterioration leaf rolling treatment; (3) fermentation treatment; (4) digestion treatment; (5) centrifugal separation treatment; (6) concentration treatment; and (7) seasoning treatment. The processing method of the fruity black tea condensed juice provided by the invention has the advantages that the rich middle and low tea fresh leaf resource and fruits at present are utilized for producing a natural fruity black tea condensed juice, the obtained fruity black tea condensed juice is red and bright in liquor color, rich in sweet fruit flavor, sweet, mellow as well as tasty and refreshing in taste, and good in mouthful, the tea pigment (theaflavin and thearubigin) content is high, a health care value is high, the limitation of tea raw material is eliminated, and the product quality is stable.

The invention discloses a separation culture method of the primary hepatocyte of a jian carp. The separation culture method comprises the following steps: selecting a healthy jian carp without injury, collecting blood from the caudal vein of the jian carp, then sterilizing the surface of a fish body, placing into a super clean bench, and aseptically collecting the liver of the jian carp; rinsing by using a PBS solution which contains double antibodies, adding a trypsin digestion solution, wherein the temperature of digestion treatment is 26-28 DEG C, and the time of the digestion treatment is 15-20 minutes; after digestion is finished, adding to a culture medium A to finish the digestion, filtering the trypsin digestion solution by using a filter screen of 200 meshes, and collecting filter liquor; and respectively centrifugalizing 50 grams of the filter liquor and 30 grams of the filter liquor for 5 minutes, then washing twice by using a culture medium B, removing a supernatant to obtain a precipitation, namely an extracted hepatocyte, preparing a cell suspension, and planking for culture. The experimental method disclosed by the invention is simple and fast and can save the separation time to a great extent. The separation time adopted by an experiment is about 40 minutes, and the obtained hepatocyte has the advantages of good growth condition, small erythrocyte quantity and cell survival rate of about 95%.

The invention discloses a preparation method for food-grade calcium hydroxide, and belongs to the field of food additives. The method comprises the following steps: pouring quicklime particles into adrum-type ball mill to obtain powdery particles with a particle size of 200-500 [mu]m, conveying the powdery particles into a quicklime bin, and conveying the obtained powdery particles into a primarydigestive device, wherein the interior of the primary digestive device is equipped with a spraying device for spraying a mixed solvent of water and an alcohol solvent, and the exterior is equipped with a cold water sleeve; controlling a primary digestion stirring frequency range at 25-40 Hz; stirring a material conveyed by the primary digestive device by a secondary digestive device until calciumoxide is fully converted into calcium hydroxide; and performing purification on the calcium hydroxide digested by the secondary digestive device to remove residues, performing drying under the temperature condition of 150-300 DEG C, and performing sieving to obtain a finished product of the required food-grade calcium hydroxide. The method provided by the invention has the beneficial effects thatmulti-stage digestion is adopted for sintering the quicklime, and compared one-stage digestion treatment, the method provided by the invention can effectively control quality and costs of the finished product of the calcium hydroxide, and improve a conversion rate and specific surface area of the calcium hydroxide.

The invention discloses a method for removing heavy metals from waste compost by united elution by ammonium sulfate and oxalic acid, mainly comprising the following steps: drying the domestic waste compost and carrying out elution on the domestic waste compost with mixed solution of the ammonium sulfate and the oxalic acid, after elution, standing for 48h; carrying out elution with water every day in the same time and lasting for 2-3 times, collecting leacheate, filtering and carrying out digestion treatment and determining the change of the contents of such heavy metals as Cd, Cr, Cu, Ni, Pb and Zn in the solution; wherein, the weight ratio of the oxalic acid of 20-40mmol / L to (NH4)2SO4 of lg / L is 1:1-2.5, and the weight ratio of the compost to the mixed solution is 1:4-10.The method of the invention belongs to the phase of mature development of elution technique and is used for technical support and preparation for pushing forward remediation work of national contaminated substrates.

The invention provides composite mulberry leaf tea. A preparation method of the composite mulberry leaf tea comprises the following steps: (1) carrying out digestion treatment or juicing treatment on raw materials, and then adding dextrin or vitamin C; (2) selecting and compounding an obtained product, and adjusting solid content; (3) measuring and taking compound liquid for later use; (4) spraying the compound liquid on mulberry leaf tea base; (5) adsorbing the compound liquid by the mulberry leaf tea base; (6) drying the mulberry leaf tea base; (7) repeating the steps (4), (5) and (6) till using up the compound liquid; (8) drying the mulberry leaf tea base, and spreading the mulberry leaf tea base for cooling, thus obtaining the finished product. The invention also provides the preparation method of the composite mulberry leaf tea. The mulberry leaf tea provided by the invention is clear and bright in tea soup and suitable for drinking by modern women and crowds with weak, cold and cool physique, is a beneficial supplement for health and has sweet mouth feel and pleasant aroma. The mulberry leaf tea is in favor of meeting the demands of consumers, promoting the satisfaction of products on the market and stabilizing the promotion of development of mulberry leaf tea industries.

The invention discloses a mud-water separation type sludge anaerobic digestion treatment system, which comprises a sludge anaerobic digestion tank, a drainage assembly and a cyclone for mud-water separation. The sludge anaerobic digestion tank is provided with a sludge inlet pipeline, a sludge circulation stirring assembly and a detection element. The cyclone is respectively connected with the drainage assembly and the sludge inlet pipeline. A mechanical stirring assembly and a biogas circulating stirring assembly are arranged in the sludge anaerobic digestion tank, the mechanical stirring assembly is arranged at the side part of a tank body, and the biogas circulating stirring assembly not only collects biogas in the tank, but also stirs sludge by using the biogas. The sludge circulating stirring assembly is used for realizing circulating stirring of sludge in the tank and discharging the sludge out of the tank. The detection element is used for detecting the liquid level, the temperature, the pressure, the viscosity and the potential of the sludge. The treatment system has the advantages of flexibility in disassembly and assembly, small occupied area, high space utilization rate and the like. The invention further discloses a sludge-water separation type sludge anaerobic digestion treatment method. The anaerobic digestion efficiency and dehydration performance of the sludge are improved, and the sludge volume is reduced.