This invention discloses a chicken
embryo fibroblast, its preparation method, and its application, relating to the field of
biopharmaceutical technology. The preparation method of the chicken
embryo fibroblast includes the following steps: S1, providing chicken embryos and obtaining the
muscle tissue of the chicken embryos; S2, mixing the
muscle tissue with a
plant-derived compound
digestion solution, and sequentially performing
digestion, purification, and
primary culture treatment using a serum-free special culture medium to obtain primary chicken
embryo fibroblasts; wherein, the
plant-derived compound
digestion solution includes bromelain and
papain. This invention, using a
plant-derived compound digestion solution and a serum-free special culture medium, significantly improves the batch-to-batch stability,
cell yield, and
cell purity of the cells, reducing the risk of exogenous
viral contamination. Therefore, the prepared chicken embryo fibroblasts exhibit high batch-to-batch stability and good reproducibility in
viral culture.