117 results about "Digestion Treatment" patented technology

The invention relates to the technical field of total phosphorus detection, in particular to a method for determining total phosphorus in water, which comprises the following steps: 1) digestion treatment: adding an oxidant into a water sample to be detected, and then performing microwave digestion treatment to obtain digested water; a molybdate reagent is added into the digested water, then an improved ascorbic acid solution is added, a chromogenic reaction is completed at the set temperature, and the improved ascorbic acid solution is obtained by mixing an ascorbic acid solution and sodium ethylene diamine tetracetate; measuring the concentration: measuring the absorbance value of the obtained color development product by using a spectrophotometer and a long-optical-path cuvette; and drawing a standard curve: using a phosphorus standard solution as a quality control sample, adding a plurality of calibration points in a low-concentration interval of 0.0-0.1 mg / L, carrying out colorimetric determination, and establishing quadratic equation fitting according to absorbance and concentration so as to determine the total phosphorus content of the water sample to be detected, and the method has the advantages of high sensitivity and good accuracy in total phosphorus testing.

The invention relates to an enzyme and bacterium combined feed additive for pre-digestion of feed raw materials as well as preparation and application of the enzyme and bacterium combined feed additive. The feed additive comprises: (a) an enzyme preparation, which is composed of protease, cellulase, xylanase, mannase, phytase, pectinase and keratinase, and has a total enzyme activity of 42500-45000 U / g; the probiotics are composed of bacillus subtilis, lactobacillus plantarum and saccharomyces cerevisiae, and the viable count is larger than or equal to 107 CFU / g; and (c) the mass ratio of enzyme to bacteria is (1-2): 1. According to the additive, the enzyme preparation and the probiotics are jointly used, and the raw materials are effectively pretreated, so that the nutritional value and the digestibility of the feed are improved. The feed additive disclosed by the invention comprises a specific enzyme preparation and probiotic combination, and predigestion treatment on the feed raw materials is realized through an optimized preparation process and fermentation process, so that the nutritional value and the utilization value of the feed raw materials are improved. The additive is simple in preparation method, low in cost and environment-friendly, and has a wide application prospect.

The invention provides a method for preparing vaterite type nanoscale calcium carbonate with ardealite as a raw material and application of the method, and relates to the technical field of inorganic nano-material preparation.The method comprises the following steps that firstly, efficient dissociation of calcium ions in the ardealite is achieved through a phase transfer method, then digestion treatment is conducted on a leaching solution, and the leaching solution is obtained; a crystal morphology regulating agent is further introduced to construct a mineralized microenvironment, and then through a carbon dioxide carbonization reaction, vaterite type nano-scale calcium carbonate with a specific average particle size (200-400 nm) is prepared. According to the preparation method, natural high polymer materials such as collagen are introduced in the preparation process to serve as green crystal form guiding agents, so that the coordination effect of the natural high polymer materials and calcium ions can be utilized, the crystal morphology can be effectively regulated and controlled, and the biocompatibility of the nano material is improved; and the application possibility of calcium carbonate in the biomedical fields of drug carriers, bone repair materials and the like is expanded.

The invention discloses a carbon fiber reinforced plastic resin insoluble component content detection method, which comprises: taking a carbon fiber reinforced plastic sample to be detected, adding a mixed solvent, placing in an ultrasonic cleaning machine, carrying out oscillation extraction, drying, placing the sample in a digestion container, carrying out a digestion reaction, carrying out suction filtration, cleaning, and drying to obtain the carbon fiber reinforced plastic resin insoluble component content. The mass of the carbon fiber sample to be detected after digestion treatment is obtained through weighing, and then the resin content of the carbon fiber reinforced plastic and the resin insoluble component content of the carbon fiber sample to be detected are calculated. The detection method provided by the invention solves the problem that the resin content and the resin insoluble component content of the same carbon fiber reinforced plastic sample cannot be detected at the same time; the method is high in detection precision, can accurately reflect the real condition of the insoluble component content of the material resin, and effectively overcomes the defects that a traditional method is greatly influenced by human factors and is high in randomness.

The invention discloses a construction and evaluation method of a liver injury organoid model, which comprises the following steps: (1) selecting mouse liver cells, perfusing a mouse with pancreatin through a sterile operation technology, removing blood in the liver, collecting liver tissues, and carrying out fine enzymolysis digestion treatment to obtain a single-cell suspension; (2) inoculating the single-cell suspension into a three-dimensional culture system, and carrying out preliminary stimulation by adopting a culture medium combined with growth factors and inhibition small molecules, so as to promote the growth, proliferation and differentiation of cells in a three-dimensional space; according to the cell growth condition, subculture operation is carried out in good time, so that continuous culture and amplification of liver organs are ensured; and (3) exposing the maturely cultured liver organoid in a stimulating factor for simulating a liver injury pathological environment, and constructing different types of liver injury organoid models. According to the liver injury organ-like model construction method, model construction can be completed in a short time, the research period is greatly shortened, the efficiency is high, and the cost is low.

The invention discloses a carrier type anaerobic sequencing batch reactor suitable for anaerobic digestion treatment of sludge and a treatment method. The anaerobic sequencing batch reactor comprises a tank body, a stirrer, a biogas water-sealed tank, a temperature adjusting module, a bottom sludge inlet valve, a scum valve, an overflow sludge discharge valve, a bottom sludge discharge valve, a circulating sludge path and carrier adding equipment, the carrier recovery device comprises a magnetic adsorption recovery device, the output end of the overflow sludge discharge valve and the output end of the bottom mud discharge valve are connected with the input end of the magnetic adsorption recovery device, the reactor can enrich microorganisms by adding the magnetic carrier, the settleability of the sludge can be improved by formed anaerobic granular sludge, and the magnetic carrier can be recycled. The device is simple in structure, realizes shorter sludge hydraulic retention time and longer sludge retention time, and is matched with carrier recovery equipment to recover carriers in discharged sludge and enable the carriers to flow back to the reactor, so that loss of the carriers is avoided.

The invention discloses a preparation method of salt-tolerant methanogen liquid for treating high-salt steroid pharmaceutical wastewater, and belongs to the technical field of anaerobic digestion treatment of high-salt pharmaceutical wastewater. According to the technology, the salt-tolerant methanogenic flora is domesticated and enriched through acetic acid and high salt dual stress, and then the salt-tolerant methanogenic bacterial liquid is prepared through passage enrichment, so that a salt-tolerant methanogenic strain is provided for biologically enhancing the anaerobic treatment performance of the high-salt steroid pharmaceutical wastewater, the COD (Chemical Oxygen Demand) removal efficiency and methanogenic efficiency of the high-salt steroid pharmaceutical wastewater are effectively improved, and the method is suitable for industrial production. The conversion of VFAs is accelerated, so that a rapid, low-cost and high-efficiency treatment method can be provided for efficient anaerobic treatment of high-salt steroid pharmaceutical wastewater.

The present application relates to a kind of method and device for producing lithium chloride using dust and lithium ore, belong to dust and sludge treatment and lithium ore lithium extraction technical field of steel enterprise.The method comprises the following steps: after lithium ore is ground, mixed sintering machine head dust and binder are added, and after adjusting the moisture content of mixed material, the mixed material is pressed ball or disc balling, the green ball is dried and then roasted, zinc, lead, lithium, potassium, rubidium, cesium in the mixture are converted into gaseous chlorides or oxides, and then collected and separated to obtain zinc, lead, lithium and other compound products.The device includes mixer, balling machine, dryer, reduction roaster and dust collector in sequence along the material transport direction.Dust is difficult to use in steel plant, and the alkali metals, zinc, lead and other elements contained therein can adversely affect blast furnace smelting, but these elements are exactly the necessary raw materials for lithium chloride extraction from lithium ore.The present application can extract and separate lithium from lithium ore, and also can simultaneously treat dust and sludge solid waste that cannot be digested and treated in steel plant.

The invention belongs to the cross technical field of veterinary medicine and biomedicine, and relates to a construction and evaluation method of a dog spontaneous breast cancer organoid model, which specifically comprises the following steps: (1) obtaining dog spontaneous breast cancer tissues through operation or biopsy, separating tumor tissues under sterile conditions, and carrying out fine enzymolysis digestion treatment to obtain dog spontaneous breast cancer tissues; obtaining a single-cell suspension; and (2) inoculating the single-cell suspension into a three-dimensional culture system, carrying out three-dimensional culture by adopting a culture medium containing EGF, FGF and B27 in combination with a growth factor, promoting the growth and amplification of the breast cancer organs, and carrying out timely passage according to the growth condition so as to maintain the activity of the model. The model constructed by the invention completely retains genetic heterogeneity and pathological microenvironment of primary tumors, does not need gene editing, is simple and convenient to operate and low in cost, is particularly suitable for individual drug sensitivity detection of pet dogs, and provides an efficient and high-simulation experimental platform for dog breast cancer mechanism research and clinical precise medication.

The utility model discloses an agricultural sludge anaerobic digestion treatment device, and relates to the technical field of sludge anaerobic digestion treatment devices. The agricultural sludge anaerobic digestion treatment device comprises a bottom plate, a control box and an anaerobic digestion reactor, a crushing mechanism is arranged at the top of the bottom plate, an extrusion mechanism is arranged on the surface of the crushing mechanism, and extraction assemblies are arranged on the top of the bottom plate, the surface of the crushing mechanism and the surface of the anaerobic digestion reactor. According to the anaerobic digestion reactor, massive sludge is crushed through the extrusion mechanism, the sludge is crushed into smaller particles through the crushing mechanism, the decomposition effect of anaerobic microorganisms is facilitated, and the sludge crushed in the crushing mechanism is extracted into the anaerobic digestion reactor through the extraction assembly; and organic matters are easier to degrade by microorganisms after the sludge is crushed, so that more biogas can be generated, the particle size can be reduced by crushing the sludge, the blocking risk is reduced, and the stable operation of the system is ensured.

The invention relates to the technical field of sewage treatment, in particular to a greenhouse gas emission control method and system applied to sewage nitrification treatment.The greenhouse gas emission control method is implemented in an aerobic treatment unit in a sewage digestion treatment system, and in the aerobic treatment unit, the sewage is subjected to nitrification treatment; arranging a front-section measuring point and a rear-section measuring point along the main flow direction of the mixed liquid, and periodically acquiring a first nitrite concentration time sequence at the front-section measuring point and a second nitrite concentration time sequence at the rear-section measuring point respectively; respectively configuring a front-section oxygen supply device and a rear-section oxygen supply device based on the front-section measuring point and the rear-section measuring point, controlling an oxygen supply base line of the front-section oxygen supply device based on the change trend of the first nitrite concentration time sequence and the second nitrite concentration time sequence, and controlling a short-time oxygen reduction control window of the rear-section oxygen supply device; wherein the oxygen supply base line is a preset oxygen supply level executed by the front-section oxygen supply device, and the short-time oxygen reduction control window carries out oxygen supply reduction or intermittent time arrangement for the rear-section oxygen supply device.

The invention discloses a processing method of pigeon blood tofu. The content of protein peptide and the absorbable utilization degree of protein in a pigeon blood tofu finished product are improved. The method comprises the following steps: (1) anticoagulant treatment: adding an anticoagulant into fresh pigeon blood, and uniformly mixing; (2) extracting the pigeon blood protein: adjusting the pH value of the anticoagulant pigeon blood to the isoelectric point of the pigeon blood protein, centrifuging, and collecting the precipitate to obtain the pigeon blood protein; (3) pre-digestion treatment: carrying out enzymolysis pre-digestion treatment on the pigeon blood protein by using pepsin and trypsin in sequence; (4) making pigeon blood bean curds: adding guar gum, carrageenan, calcium chloride and water into the pre-digested pigeon blood protein, performing reflux treatment by using a colloid mill, and then heating gel to obtain the pigeon blood bean curds; (5) inoculating and fermenting: inoculating debaryomyces hansenii suspension into the pigeon blood tofu, and fermenting; and sterilizing the fermented pigeon blood tofu with steam, and cooling to obtain the pigeon blood tofu product.

The invention discloses a method for synergistically preparing magnesium ammonium phosphate by treating ammonia-nitrogen wastewater with phosphate tailings, which comprises the following steps: roasting the phosphate tailings at high temperature to obtain roasted ore; the obtained roasted ore is subjected to digestion treatment, CO2 is introduced into ore pulp for carbonization, and the CO2 comes from roasting waste gas CO2 and carbon-containing flue gas; performing solid-liquid separation to obtain a first filtrate and a first filter residue, using the first filter residue to recover calcium, and using the first filtrate to treat ammonia-nitrogen wastewater; adding a phosphorus source and ammonia-nitrogen wastewater into the first filtrate, adjusting the pH value, and filtering after reaction to obtain a second filtrate and a second filter residue; the ammonia nitrogen content of the second filtrate is detected through Nessler's reagent spectrophotometry, and the ammonia nitrogen removal standard is met; and drying the second filter residue at low temperature to obtain a magnesium ammonium phosphate product. The method is low in production cost, high in product value and high in resource utilization rate, ammonia nitrogen in the wastewater can be efficiently removed, the environmental wastewater discharge standard is met, efficient value-added utilization of industrial by-products is achieved, and meanwhile the prepared magnesium ammonium phosphate product meets the industrial standard.

The application belongs to the technical field of sludge treatment, and discloses a continuous ultrasonic sludge digestion device and a treatment method thereof, which comprises a treatment outer cylinder, an inlet pipe fixedly connected to the top of the treatment outer cylinder, and a rotating treatment assembly rotatably installed in the treatment outer cylinder. In the process of rotating the ultrasonic composite treatment assembly along with the treatment inner cylinder, the magnetic pole arrangement of the arc-shaped magnetic block one and the arc-shaped magnetic block two causes the intermittent overturning of the ultrasonic composite treatment assembly, and the sludge in the treatment cavity is stirred and subjected to the reversing ultrasonic effect. On the one hand, the internal sludge is stirred by a mechanical method, the sludge is uniformly distributed, sedimentation is avoided, and the uniform digestion effect of the ultrasonic transducer on the sludge is improved. On the other hand, the output direction of the ultrasonic transducer distributed around is dynamically changed, the uniformity of the sludge is provided, the sludge digestion uniformity is comprehensively improved, the actual digestion efficiency is improved, local sedimentation and omission are avoided, and the actual digestion treatment effect is greatly improved.

The invention relates to a kit for constructing a sequencing library, and the kit comprises a repair treatment reagent which comprises a first enzyme with a repair function, NAD < + >, ATP, dNTPs and a first buffer solution; the connection treatment reagent comprises a second enzyme with a connection function, a joint and a second buffer solution; the digestion treatment reagent comprises a third enzyme with a digestion function; the first buffer solution comprises 10 to 50 mM of a Tris buffer solution, 5 to 120 mM of monovalent cations and 0.1 to 10 mM of divalent cations; and the second buffer solution comprises 20 to 100 mM of a Tris buffer solution, 5 to 20 mM of divalent cations and 1 to 10 mM of dithiothreitol. The kit provided by the embodiment of the invention is simple in composition, convenient to use and high in library yield, the library building cost is remarkably reduced, and wide application of three-generation sequencing can be powerfully promoted.

The application provides an amniotic membrane stem cell and a separation method and a hydrogel 3D culture method thereof, relates to the technical field of cell culture, and comprises the following steps: placing amniotic membrane fragments in a pre-digestion buffer in sequence and incubating for 10-20 min, placing the amniotic membrane fragments in a mixed enzyme solution and performing digestion treatment, filtering, and obtaining a filtrate and residual amniotic membrane fragments; centrifuging the filtrate to obtain a cell precipitate, and performing differential adhesion culture to obtain amniotic membrane epithelial stem cells; adding a culture medium to resuspend the residual amniotic membrane fragments so that the residual amniotic membrane fragments are in contact with a culture container and are in a non-suspended state, performing adhesion culture, and obtaining amniotic membrane mesenchymal stem cells that have climbed out. Compared with the number of cells and the cell survival rate of cells that are subjected to digestion treatment of other mixed enzymes, the number of cells obtained by digestion treatment of the mixed enzyme is significantly increased, and the cell survival rate is higher; compared with a step-by-step digestion strategy, the mixed enzyme digestion one-step method is more simple, and efficient and synchronous separation is achieved. The technical problem of a significant decrease in cell activity caused by the step-by-step digestion separation strategy in the prior art is solved.

The invention provides a large-scale epinephelus lanceolatus breeding method, and relates to the technical field of epinephelus lanceolatus breeding, the method comprises the steps of fertilized egg digestion treatment and indoor and outdoor relay breeding, and the fertilized eggs are subjected to digestion treatment, so that membrane rupture of fish flowers can be facilitated; in the indoor breeding process, the calamus eggs obtained after fry strengthening are fed, more nutrient substances can be provided for the fry, and the fry can open the mouth easily; fish flowers are cultured indoors when the bodies of the fish flowers are weak in the early metamorphosis stage, the fish flowers are cultured in an outdoor pond after having certain resistance, the resistance of the fish fries can be effectively improved, in addition, during outdoor culture, the fish fries can be prevented from being affected by pathogenic microorganisms while the water body is continuously disinfected and ammonia nitrogen is removed through an electrolytic seawater chlorine dioxide generator, and the fish fries are effectively prevented from being damaged. Under the action, the seedling survival rate can be improved.

A water treatment method treats raw water (water to be treated) containing organic wastewater. The method includes (i) an aeration process performs an initial absorption treatment by aerating the water to be treated, (ii) a filtration process for filtering the water to be treated which has been treated by the initial absorption treatment in the aeration process, (iii) a digestion treatment process for digesting solids captured by the filtration process, (iv) a biological treatment process for denitrifying, with activated sludge, filtered water obtained through the filtration process, (v) a sludge transfer process for sending the activated sludge from the biological treatment process to the aeration process, and (vi) an adjustment process for adjusting an amount of the activated sludge sent to the aeration process via the sludge transfer process based on a nitrogen concentration of treated water which has been biologically treated by the biological treatment process.

The application discloses a method for digesting and separating fluorinated aluminum by program, which comprises the following steps: placing a catalyst with metal elements supported on alumina as a carrier into a reaction container of a cluster digestion device, adding hydrochloric acid and / or nitric acid, setting a digestion program, introducing inert gas for pre-pressurization, performing primary digestion, and simultaneously starting a condensation reflux atomizer; after the program is completed, adding hydrofluoric acid, setting a digestion program, and performing secondary digestion; after the digestion is completed, entering a cooling and crystallization program, performing cooling and crystallization, and separating to obtain a sample solution to be detected and a solid; the solid is dried and calcined to obtain fluorinated aluminum crystals, and the liquid is used for detecting the content of metal elements. The method is applied to the digestion treatment of the alumina sample with metal elements supported thereon, has a large sample treatment range, complete digestion, no volatile loss of elements, and can separate AlF3 in an alpha crystal form through the program digestion of the digestion device.

The application discloses a preparation process of rice wine fermentation and relates to the technical field of rice wine preparation, and comprises the following steps: S1, a circulating fermentation activator is prepared; S2, the circulating fermentation activator is introduced into a digestion treatment procedure, a porous carrier is loaded with non-amine-producing competitive lactic acid bacteria biofilm and covalently immobilized amine oxidase; S3, according to the risk level of a coupling risk value, a corresponding digestion strengthening procedure is triggered; and S4, the circulating fermentation activator treated by the digestion treatment procedure is added back to a new batch of fermentation substrate. The application prepares the circulating fermentation activator by directional complex enzymolysis treatment of the distiller's grains generated after rice wine fermentation, and the non-amine-producing competitive lactic acid bacteria biofilm and the covalently immobilized amine oxidase are loaded on the porous carrier, so that the biological amine in the circulating system is subjected to directional digestion, and the problems of excessive accumulation of biological amine and deterioration of rice wine flavor caused by direct reuse of the distiller's grains in the prior art are solved.

The utility model provides a heat digestion treatment device for element form valence state change, including casing, digestion pool, buffer pool, reaction pipeline, circulating pipeline, liquid circulating device and heating device, and is provided with oil containing cavity in digestion pool and buffer pool, and heating device is installed in oil containing cavity, and liquid circulating device is connected on any circulating pipeline for driving liquid circulation flow between digestion pool and buffer pool. Through setting up oil containing cavity to contain heat conducting oil, heat conducting oil is used as heat transfer medium to heat reaction pipeline, improves the temperature of heating, and has strong digestion capacity, on the other hand, sets up buffer pool and communicates buffer pool with digestion pool through circulating pipeline, and liquid circulating device drives heat conducting oil circulation flow between buffer pool and digestion pool, thereby makes heat conducting oil heating temperature rise more stable.

The invention discloses an MEC reactor for promoting sludge deamination and strengthening anaerobic digestion capability by coupling oxides, and relates to the field of anaerobic digestion treatment of sludge in a sewage treatment plant and resource utilization of biogas, the MEC reactor comprises an MEC reactor, an oxidation-reduction potential detection device, a hydrogen detection device, a PLC control module and an oxide adding device; the oxide adding device takes air as an oxide, the air is converted into micro-nano bubbles through a microporous aerator, and ammonia nitrogen is oxidized into nitrate nitrogen; oRP and hydrogen partial pressure in the MEC reactor are monitored in real time through the oxidation-reduction potential detection device and the hydrogen detection device respectively and transmitted to the PLC control module, and the ORP and the hydrogen partial pressure in the MEC reactor main body are intelligently and accurately controlled according to a negative feedback regulation mechanism. The MEC reactor disclosed by the invention relieves the inhibition effect of partial pressure of ammonia nitrogen and hydrogen on methanogens while deaminizing the sludge, improves the biogas yield and strengthens the anaerobic digestion capability of the MEC reactor.

The application belongs to the technical field of resource utilization, and particularly relates to a method for using lime plant dedusting ash slurry for neutralization in hydrometallurgy and application thereof. The method for using lime plant dedusting ash slurry for neutralization in hydrometallurgy comprises the following steps: S1, performing digestion treatment on the dedusting ash collected by the lime plant; S2, preparing dedusting ash slurry; S3, adding the prepared dedusting ash slurry into acidic ore slurry or tailings generated in the hydrometallurgy process, adjusting the pH of the ore slurry, and separating the ore slurry into solid and liquid to complete the neutralization of the acidic ore slurry or tailings. The dedusting ash of the lime plant is used as raw material, and the dedusting ash is treated through a specific method. The prepared dedusting ash slurry is used for the neutralization of the acidic ore slurry and tailings generated in the hydrometallurgy process. The method can achieve good neutralization effect, and the reaction is mild and smooth, which can reduce the risk of local high pH. The use of the dedusting ash can realize the resource utilization of the dedusting ash of the lime plant, and has good environmental protection benefits.

The application discloses a method for replacing high-temperature denaturation to produce single strands in a multiplex PCR detection reaction and a method for improving the sensitivity and accuracy of liquid-phase hybridization detection of multiplex PCR products and a matching kit. The method mainly comprises the following steps: a 5' end of a primer is designed and modified to have a phosphate group, so that a PCR product with a phosphorylated end of one strand is amplified; and the PCR amplification product is subjected to digestion treatment by using Lambda exonuclease, so that the DNA strand with the phosphorylated end is completely removed, and a single strand is obtained. The method for replacing high-temperature denaturation to produce single strands in the ordinary multiplex PCR detection reaction eliminates the negative influence of DNA recombination on hybridization of a probe and a target sequence, improves the hybridization efficiency, and improves the accuracy and sensitivity of a liquid chip technology platform in application of molecular detection.

The application discloses a kind of closed field leachate and kitchen garbage combined anaerobic digestion device and method, device includes sequentially and side by side and is connected and is arranged pretreatment bin, preliminary mixing bin and anaerobic digestion bin, preliminary mixing bin middle part is equipped with a for receiving the mixed component of solid kitchen garbage on the filter plate.S1, kitchen garbage pretreatment;S2, kitchen garbage humification treatment;S3, combined anaerobic digestion treatment;S4, gas collection.The application is by closed field leachate as inoculum, kitchen garbage is digestion material, carries out sequential batch combined anaerobic digestion treatment, so that closed field leachate has good inoculation function, under the condition that no any inoculum is added, combined anaerobic digestion system can quickly enter gas production peak period finally by kitchen garbage conversion formed humus is substantially completely degraded.

Provided is a method for preparing a recombinant human collagen. The method comprises: constructing a genetically engineered bacterium which expresses a prolyl hydroxylase and a recombinant human collagen; culturing the genetically engineered bacterium and performing digestion treatment by means of pepsin to obtain a recombinant human collagen type I or type III. The prolyl hydroxylase comprises P4Ha2, P4Hb and vP4H, and by means of controlling the ratio of the copy numbers of the genes thereof, the recombinant human collagen and a natural human collagen are consistent in hydroxylation modification. Also provided is a large-scale production method for the recombinant human collagen. The prepared recombinant human collagen has completely the same sequence composition and length and substantially the same hydroxyproline content as the natural human collagen, and also has a stable full-length triple helix structure and biological activity without immunogenicity and potential risk of viruses.

The application relates to a method for separating and primary culturing of monopterus albus liver cells. The method comprises the following steps: obtaining monopterus albus liver tissue, perfusing the liver tissue with HBSS buffer solution without calcium and magnesium until blood is completely drained; removing the capsule, blood clots and connective tissue after rinsing, and cutting the liver tissue; sequentially using 0.02% EDTA solution, 0.25% trypsin solution and 50 U / mL collagenase IV working solution to perform three-step digestion treatment on the liver tissue fragments; obtaining a cell suspension by blowing and filtering after the digestion is completed, and then performing gradient centrifugal purification of the cells at rotating speeds of 600 rpm, 250 rpm and 80 rpm in sequence; and finally resuspending the cells in DMEM / F12 culture medium containing 10% FBS and inoculating and culturing the cells. The monopterus albus primary liver cells obtained by the method have the advantages of large quantity, high activity and high purity, and provide a reliable in-vitro model for monopterus albus related research.

The application discloses a method for preparing stem cell exosomes by using multiple pulse electric field stimulation, which comprises the following steps: step 1, thawing the frozen stem cells and preparing cell suspension, and adjusting the cell density to a first cell density preset value; step 2, when the cell fusion degree in the cell suspension reaches a first cell fusion degree preset value, the cell suspension is subjected to digestion treatment; step 3, placing the cell suspension after digestion treatment in an electric conversion cup, and using a nanosecond pulse electric field with a preset electric field intensity to stimulate the cell suspension in the electric conversion cup; step 4, collecting the cell supernatant in the culture dish for exosome extraction within a first preset time length after the nanosecond pulse electric field pretreatment, meanwhile, adding culture medium in the culture dish again and culturing for a preset time length; and step 5, repeating steps 2 to 4 until the cells reach a preset generation number.

The utility model relates to a biodegradable sludge treatment device which comprises a pretreatment mechanism, a microbiological treatment mechanism and a digestion treatment mechanism, and the pretreatment mechanism is used for pretreating sewage; the microbiological treatment mechanism comprises a microbiological treatment assembly and a secondary sedimentation tank, the microbiological treatment assembly is used for purifying the pretreated sewage, a water outlet of the microbiological treatment assembly is communicated with the secondary sedimentation tank, and the secondary sedimentation tank is used for discharging supernatant liquid and precipitating sludge; the digestion treatment mechanism comprises a sludge collecting tank, a sludge pump and an advanced treatment assembly, a sludge outlet of the secondary sedimentation tank is communicated with the sludge collecting tank, the sludge pump is mounted in the sludge collecting tank and discharges sludge in the sludge collecting tank into the advanced treatment assembly, and the advanced treatment assembly degrades the sludge. According to the technical scheme, the digestion treatment mechanism can degrade the settled sludge, so that the total amount of the sludge is reduced by 90% or above, the amount of the sludge needing to be incinerated or landfilled is reduced, and the pollution to the environment is reduced.