35 results about "Methicillin resistance gene" patented technology

Disclosed are methods of identifying a methicillin-resistant Staphylococcus aureus (MRSA) or methicillin-sensitive Staphylococcus aureus (MSSA) in a sample. The present invention provides a diagnostic method comprising modification of sequences of S. aureus by converting non-methylated cytosine residues ultimately into thymidine residues in the target nucleic acid. The invention further provides for the detection of modified sequences derived from the spa gene, the mecA gene, and the integrated SCCmec cassette of S. aureus.

The invention relates to a double-stranded small molecular interference nucleic acid used for inhibiting and killing various drug-resistant bacteria represented by methicillin-resistant Staphylococcus aureus. The siNA described in the present invention is a double-stranded molecule with 19 base pairs, the sense strand and the antisense strand each have two protruding bases dT at the 5' end, and the GC content is 40-55%; the targeted sequence Selected from genes related to life activities such as replication, transcription, and translation in the genome of Staphylococcus aureus, and the mecA gene related to drug resistance; the target sequence is conserved in more than 90% of Staphylococcus aureus strains and is compatible with All gene sequences in the human genome have different sequence regions; the target sequence of the siNA double-stranded molecule is selected from SEQ ID NO.1-325, the sense strand is a DNA or RNA sequence corresponding to the target sequence one-to-one, and the antisense The strand is the RNA or DNA that corresponds one-to-one to the sequence of the sense strand according to the principle of base complementarity.

The invention discloses a pyrophosphate detection kit for common pathogens and drug-resistant genomes and its detection method and application, wherein the kit is used to detect 12 drug-resistant bacterial strains and drug-resistant genes in samples to be tested, Including Pseudomonas aeruginosa, Acinetobacter baumannii, Staphylococcus aureus, Klebsiella pneumoniae, Stenotrophomonas maltophilia, Escherichia coli, Enterococcus faecalis, Enterococcus faecium, KPC gene, The genotypes of OXA-23 gene, SHV gene and MecA gene, the kit includes amplification primer set, sequencing primer set, amplification solution, amplification enzyme and sequencing substrate. The present invention uses the pyrosequencing method to detect 12 highly pathogenic and high-detection rate pathogenic bacteria and drug-resistant genes at the same time. Detection, the detection process is flexible and simple, and the pyrosequencing results are fast and readable.

Provided is a primer pair of primers for methicillin-resistant gene detection for the purpose of achieving highly sensitive methicillin-resistant gene detection. Said primer pair comprises a combination of SEQ ID NO:3 and SEQ ID NO:7, a combination of SEQ ID NO:2 and SEQ ID NO:9, a combination of SEQ ID NO:1 and SEQ ID NO:8, a combination of SEQ ID NO:1 and SEQ ID NO:9, a combination of SEQ ID NO:4 and SEQ ID NO:11, a combination of SEQ ID NO:5 and SEQ ID NO:12, a combination of SEQ ID NO:6 and SEQ ID NO:10, or a combination of SEQ ID NO:6 and SEQ ID NO:12.

The present invention provides improved tests for the detection of methicillin-resistant Staphylococcus aureus bearing a variant mecA gene. The tests are particularly useful for eliminating certain false negative results due to the presence of this variant in MRSA in patient samples.