Ultra-sensitive electrochemical LCR sensor for detecting methicillin-resistant staphylococcus aureus in synovial fluid

A methicillin and staphylococcus-resistant technology, applied in the field of electrochemical LCR sensors, can solve the problems of difficulty in distinguishing single bases, false positives and false negatives, etc., and achieves low detection cost, simple detection operation process, and high sequence sensitivity. Effect
CN112708684APending Publication Date: 2021-04-27THE FIRST AFFILIATED HOSPITAL OF FUJIAN MEDICAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
THE FIRST AFFILIATED HOSPITAL OF FUJIAN MEDICAL UNIV
Publication Date
2021-04-27

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Abstract

The invention discloses an ultra-sensitive electrochemical LCR sensor for detecting methicillin-resistant staphylococcus aureus in synovial fluid. The ultra-sensitive electrochemical LCR sensor comprises the following steps of (1) designing two specific double-stranded short probes (S-dsDNA) according to conservative series of a MecA gene; (2) in an LCR reaction system, taking the MecA gene as a template, connecting two short series of probes through DNA ligase to form long double-stranded DNA (L-dsDNA), and then carrying out cyclic amplification by using an L-dsDNA template to form a large amount of L-dsDNA, and (3) fixing the formed L-dsDNA with sulfydryl and biotin modification on a BSA modified gold electrode through a gold-sulfur bond, dropwise adding avidin-PolyHRP to be combined with biotin on the L-dsDNA, finally putting the electrode into a base solution containing TMB and H2O2, and enabling H2O2 to oxidize TMB to generate an electrochemical signal under the catalysis of HRP. The sensor has the advantages of economy, quickness, high sensitivity, specificity and the like, can be used for detecting a single base mutation series, and realizes the detection of the MRSA in the synovial fluid of a patient infected around the joint prosthesis.
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Description

technical field

[0001] The invention relates to an ultra-sensitive electrochemical LCR sensor for detecting methicillin-resistant Staphylococcus aureus (MRSA) in the joint fluid of patients with joint prosthetic infection, especially relates to an electrochemical sensing technology based on LCR to detect joint Methods for methicillin-resistant Staphylococcus aureus (MRSA) in the joint fluid of patients with periprosthetic infections. Background technique

[0002] Hip and knee periprosthetic infection (PJI) is a catastrophic and serious complication after artificial joint replacement, which occurs in about 1%-2% of patients after artificial joint replacement. Most PJIs are caused by Gram-positive bacteria including Staphylococcus species. Several studies have shown that about 74% of PJI patients are caused by methicillin-resistant Staphylococcus aureus (MRSA) infection. The traditional method of detecting MRSA is through bacterial culture. However, because many PJI patient...

Claims

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