20 results about "Biotin binding" patented technology

The invention provides an immobilized carrier based on bovine serum albumin-biotin as well as a preparation method and application of the immobilized carrier. The biological sample fixing carrier comprises a substrate and a composite layer of bovine serum albumin coupled biotin and neutral avidin, wherein the composite layer is fixed on the surface of the substrate; the bovine serum albumin coupled biotin and neutral avidin composite layer is connected to the surface of the substrate after being co-incubated with the substrate through bovine serum albumin coupled biotin, and then the neutral avidin is combined with biotin in the bovine serum albumin coupled biotin through co-incubation with the neutral avidin; the bovine serum albumin coupled biotin and neutral avidin composite layer is fixed on the surface of the substrate. The biomolecules on the surface of the carrier are high in fixing efficiency, good in uniformity, high in adaptability, high in signal-to-noise ratio and easy to operate.

The invention relates to the technical field of biology, in particular to multi-layer streptavidin coated magnetic particles and a preparation method and application thereof. A self-assembly mode is adopted, NHS-PEG (n)-NHS is used as a connecting arm between streptavidin layers, when streptavidin molecular layers are increased, the biotin binding amount of the magnetic particles is remarkably increased, the space structure is enlarged through the PEG connecting arm between the streptavidin molecular layers, the steric hindrance between streptavidin molecules can be effectively reduced, and the biotin binding capacity of the magnetic particles is improved. Therefore, the difficulty of capturing target macromolecules is obviously reduced. Meanwhile, the magnetic particles coated by the process have obvious advantages in the aspect of dispersity. The streptavidin magnetic particles coated by the method can be applied to a magnetic particle chemiluminescence detection platform and used in an immunodetection kit.

Disclosed herein are chromatographic materials comprising streptavidin molecules, wherein the streptavidin molecules are bound to biotin and one or more biotinylated affinity agents. The biotinylated affinity agent may be a biotinylated antibody, biotinylated antigen-binding fragment, or biotinylated oligonucleotide. The streptavidin chromatographic materials may be used to prepare affinity chromatographic materials. Due to biotin binding to the conjugated streptavidin, the materials provided herein have reduced to no streptavidin leachate, resulting in improved performance.

The present invention relates to a composition containing a fusion peptide having tripeptide and biotin bound to each other therein as an active ingredient which is effective for skin whitening, antioxidation, skin condition improvement, hair loss prevention or alleviation, and hair growth promotion. The fusion peptide in which tripeptide and biotin are bound to each other according to the present invention inhibits melanin biosynthesis, promotes cell proliferation, enhances antioxidative activity, and provides effects such as skin whitening, skin regeneration, wound healing, hair loss suppression, and hair growth promotion, and thus can be utilized as a material for cosmetics, pharmaceuticals, and food products.

The present disclosure generally relates to a method for forming a cis divalent Streptavidin (SA) complex, the complex comprising a Streptavidin core and a biotin structure arranged to connect to two biotin binding sites of the Streptavidin core, wherein the biotin structure has been specifically arranged such that at least one biotin binding site on the Streptavidin core remains unoccupied. The present disclosure also relates to a corresponding method for forming a mixture comprising the cis divalent Streptavidin (SA) complex, and to a method for forming a composition comprising such a cis divalent Streptavidin (SA) complex.

The invention discloses a high-sensitivity detection method and a high-sensitivity detection kit for soluble CD163 in urine. According to the method, a rare earth ion europium labeled streptavidin is adopted, an antigen is captured through a CD163 antibody on a stationary phase coated plate, then the antigen is combined with a biotinylated antibody to form a solid-phase antibody-antigen-biotinylated antibody sandwich immune complex, and finally the europium labeled streptavidin is added to be combined with biotin on the biotinylated antibody to form a solid-phase antibody-antigen-biotinylated antibody sandwich immune complex. The method comprises the following steps: adding a solid-phase antibody, an antigen, a biotinylated antibody and europium labeled streptavidin into a sandwich immune complex to form a solid-phase antibody-antigen-biotinylated antibody-europium labeled streptavidin sandwich immune complex, exciting europium ions to emit fluorescence through a time-resolved immunofluorescence analyzer, amplifying a fluorescence signal by an enhancement solution, and detecting by the time-resolved immunofluorescence analyzer to obtain the fluorescence intensity in a standard substance and a sample pore plate. And calculating to obtain the usCD163 content in the corresponding sample. The invention provides a novel detection method for IgA diagnosis, and has the characteristics of high sensitivity and simplicity in operation.

Functionalizing a sensor surface with a polydopamine or polynorepinephrine coating covalently bonded to a multi-arm poly(ethylene oxide) that is covalently bonded to a deglycosylated native avidin protein having available biotin binding sites for functionalization with biotinylated aptamers or proteins / peptides that can selectively bind to target biomolecules.

Disclosed herein are chromatographic materials comprising crosslinked streptavidin particles, wherein the crosslinked streptavidin molecule is conjugated to the surface material of a particle, said particle comprising a polymer core. The crosslinked streptavidin particles are bound to a biotinylated affinity agent and free biotin. The combined impact of streptavidin crosslinking and binding free biotin to accessible binding sites in streptavidin drastically reduce leaching of streptavidin from the chromatographic materials. The chromatographic materials disclosed herein may be used to prepare affinity chromatographic columns..

The invention relates to an Alzheimer's disease blood marker flow type fluorescence luminescence multi-joint detection kit and application thereof. The Alzheimer's disease blood marker flow type fluorescence luminescence multi-joint detection kit comprises a capture microsphere mixed solution, a detection antibody mixed solution, a streptavidin-phycoerythrin conjugate, an interference elimination solution and a washing solution. The bihexanamide-biotin is labeled on the detection antibody, the steric hindrance of combination of the streptavidin-phycoerythrin conjugate and the biotin on the detection antibody is reduced through bihexanamide, more phycoerythrin can be combined on a single antibody, a fluorescence signal is remarkably enhanced, and the detection sensitivity is greatly improved. An interference elimination solution containing dimethyl sulfoxide and Proclin300 at the same time is adopted and mixed with a plasma sample, then a capture antibody and a detection antibody are added, non-specific adsorption of lipids and binding proteins thereof in plasma on the surfaces of microspheres is weakened, and the detection accuracy is greatly improved. According to the method, a machine learning model based on a logistic regression algorithm is constructed through a joint detection result of seven markers in three layers, and the judgment accuracy is cooperatively improved.

The present application relates to the field of biotechnology, especially to a multi-layer streptavidin coated magnetic microparticle, a preparation method and application thereof. The present application uses a self-assembly method, and uses NHS-PEG(n)-NHS as a connecting arm between streptavidin layers. When the streptavidin molecular layer is increased, the amount of biotin combined with the magnetic microparticle is significantly improved. The PEG connecting arm between the streptavidin molecular layers increases the spatial structure, effectively reduces the spatial steric hindrance between the streptavidin molecules, and thus significantly reduces the difficulty of capturing target macromolecules. At the same time, the magnetic microparticle coated by the process has obvious advantages in dispersibility. The streptavidin magnetic microparticle coated by the method can be applied to a magnetic microparticle chemiluminescence detection platform and used in an immunological detection kit.

The invention relates to the technical field of biological medicine, in particular to a preparation method of a CAR-T cell targeting CD248, the CAR-T cell targeting CD248 comprises a CAR targeting CD248, the CAR targeting CD248 sequentially comprises CD8 alpha, a biotin recognition structural domain, CD8 TM, CD28, CD137 and CD3 zeta from the N end to the C end, the amino acid sequence of the CAR targeting CD248 is shown as SEQ ID NO.1, and the coded DNA sequence of the CAR targeting CD248 is shown as SEQ ID NO.2. The invention further discloses a preparation method of the CAR-T cell targeting CD248. Biotin binding immune receptor T cells are designed for targeting CD248, myocardial fibrosis can be effectively inhibited, and the cardiac function is improved.

The present invention relates to a monoclonal antibody capable of binding to biotin. In one embodiment the monoclonal antibody according to the invention also does not bind to a biotin moiety on a biotinylated molecule, wherein the biotin moiety is attached to the molecule via the carbon atom of the carboxyl function of the valeric acid moiety of biotin. Also disclosed is a method for generation of an antibody as disclosed herein. The monoclonal antibody according to the invention is of specific use in a method for measuring an analyte in a sample, wherein a (strept)avidin / biotin pair is used to bind a biotinylated analyte specific binding agent to a (strept)avidin coated solid phase.

Biotin-containing monomers, polymeric materials formed from the biotin-containing monomers, articles containing the polymeric materials, methods of making the articles, and methods of using the articles are provided. The articles can be used, for example, for affinity capture of biotin-binding proteins, including biotin-binding fusion proteins (i.e., a biotin-binding protein fused to another biomaterial). Articles that contain captured biotin-binding proteins can be further used for affinity capture of various biotin-containing biomaterials such as biotinylated proteins. The articles can also be used, for example, for affinity capture of biotin-binding fusion proteins where the fusion protein includes, for example, an enzyme or antibody.

The present invention relates to a monoclonal antibody capable of binding to biotin. In one embodiment the monoclonal antibody according to the invention also does not bind to a biotin moiety on a biotinylated molecule, wherein the biotin moiety is attached to the molecule via the carbon atom of the carboxyl function of the valeric acid moiety of biotin. Also disclosed is a method for generation of an antibody as disclosed herein. The monoclonal antibody according to the invention is of specific use in a method for measuring an analyte in a sample, wherein a (strept)avidin / biotin pair is used to bind a biotinylated analyte specific binding agent to a (strept)avidin coated solid phase.

The present invention relates to a gene construct characterized by encoding a fusion protein of flgM and Strepavidin having biotin binding ability through the linkage of an anti-sigma factor flgM gene and a gene encoding streptavidin, which enables expression and secretion of a streptavidin-fusion protein with biotin binding ability in an anti-cancer strain capable of targeting tumor sites and stimulating immune activity, and to an anti-cancer adjuvant and tumor imaging agent that, when transformed by this construct, exhibits anti-cancer activity itself and can be effectively utilized in the diagnosis and treatment of tumor cells together with anti-cancer substances or contrast agents labeled with biotin, which specifically interacts with streptavidin.

The invention relates to the technical field of gene therapy, and discloses an AMD gene therapy AAV biological distribution ELISA detection method, which comprises the following steps: pre-coating an ELISA plate with a capture antibody (18B2G9) to capture the antibody, adding a sample, incubating to specifically bind a substance to be detected with the capture antibody, washing off uncombined substances, adding excessive VEGF to bind with the substance to be detected, washing off uncombined VEGF, and detecting the AAV biological distribution by using the ELISA plate. According to the method, Biotin-labeled Anti-VEGF is combined with VEGF to form a bridging compound, SA-HRP is added to be combined with biotin on the bridging compound, TMB is added for color development, a reaction is terminated through sulfuric acid, and a light absorption value of a reaction hole is read through a microplate reader. The detection method is high in sensitivity, good in specificity, accurate and reliable, and is used for preclinical biological sample detection of gene therapy products.

A programmable cell receptor complex expressed by an immunocyte, wherein the programmable receptor complex includes a plurality modified receptor subunits, wherein the modified receptor subunits have been engineered or modified to include FcγRI receptor components, biotin-binding components, or both, and wherein the FcγRI receptor components and biotin-binding components are operative to bind to target detector molecules that bind to or otherwise interact with predetermined targets.

The present invention generally relates to method for forming an immunolabeling complex, the immunolabeling complex comprising a labeled monovalent biotin-binding composition. The invention also related to the use of the antibody-reporter molecule complex for detecting a target in a sample.

Technologies for the prevention and / or treatment of pathogenic or microbial infections. Compositions relate to compositions, vaccines and methods comprising an immunogenic multiple antigen presenting system (MAPS), where two or more biotinylated polysaccharide antigens are interconnected or joined together by one or more fusion proteins comprising (i) a sialic acid binding domain (SBD) and (ii) a biotin-binding moiety (BBM), thereby facilitating the joining multiple polysaccharide antigens together in the complex to form a cross-linked MAPS-X immunogenic complex. The polysaccharide antigens that are linked can be on the same polysaccharide macromolecule or on distinct polysaccharide macromolecules.

The present invention relates to a gene construct characterized in that an anti-sigma factor flgM gene and a gene encoding streptavidin are linked to encode a fusion protein of flgM and Stv with biotin-binding capacity, which enables the expression and secretion of a streptavidin fusion protein with biotin-binding capacity in an anticancer strain that targets tumor regions and can stimulate immune activity, as well as an antineoplastic adjuvant and a tumor imaging aid which not only itself has antineoplastic activity, but can also be used usefully for the diagnosis and treatment of tumor cells in conjunction with antineoplastic agents or contrast agents labeled with biotin that specifically reacts with streptavidin.