9 results about "Biotin binding" patented technology

Disclosed herein are chromatographic materials comprising streptavidin molecules, wherein the streptavidin molecules are bound to biotin and one or more biotinylated affinity agents. The biotinylated affinity agent may be a biotinylated antibody, biotinylated antigen-binding fragment, or biotinylated oligonucleotide. The streptavidin chromatographic materials may be used to prepare affinity chromatographic materials. Due to biotin binding to the conjugated streptavidin, the materials provided herein have reduced to no streptavidin leachate, resulting in improved performance.

Disclosed herein are chromatographic materials comprising crosslinked streptavidin particles, wherein the crosslinked streptavidin molecule is conjugated to the surface material of a particle, said particle comprising a polymer core. The crosslinked streptavidin particles are bound to a biotinylated affinity agent and free biotin. The combined impact of streptavidin crosslinking and binding free biotin to accessible binding sites in streptavidin drastically reduce leaching of streptavidin from the chromatographic materials. The chromatographic materials disclosed herein may be used to prepare affinity chromatographic columns..

The invention relates to an Alzheimer's disease blood marker flow type fluorescence luminescence multi-joint detection kit and application thereof. The Alzheimer's disease blood marker flow type fluorescence luminescence multi-joint detection kit comprises a capture microsphere mixed solution, a detection antibody mixed solution, a streptavidin-phycoerythrin conjugate, an interference elimination solution and a washing solution. The bihexanamide-biotin is labeled on the detection antibody, the steric hindrance of combination of the streptavidin-phycoerythrin conjugate and the biotin on the detection antibody is reduced through bihexanamide, more phycoerythrin can be combined on a single antibody, a fluorescence signal is remarkably enhanced, and the detection sensitivity is greatly improved. An interference elimination solution containing dimethyl sulfoxide and Proclin300 at the same time is adopted and mixed with a plasma sample, then a capture antibody and a detection antibody are added, non-specific adsorption of lipids and binding proteins thereof in plasma on the surfaces of microspheres is weakened, and the detection accuracy is greatly improved. According to the method, a machine learning model based on a logistic regression algorithm is constructed through a joint detection result of seven markers in three layers, and the judgment accuracy is cooperatively improved.

The present application relates to the field of biotechnology, especially to a multi-layer streptavidin coated magnetic microparticle, a preparation method and application thereof. The present application uses a self-assembly method, and uses NHS-PEG(n)-NHS as a connecting arm between streptavidin layers. When the streptavidin molecular layer is increased, the amount of biotin combined with the magnetic microparticle is significantly improved. The PEG connecting arm between the streptavidin molecular layers increases the spatial structure, effectively reduces the spatial steric hindrance between the streptavidin molecules, and thus significantly reduces the difficulty of capturing target macromolecules. At the same time, the magnetic microparticle coated by the process has obvious advantages in dispersibility. The streptavidin magnetic microparticle coated by the method can be applied to a magnetic microparticle chemiluminescence detection platform and used in an immunological detection kit.

The present invention relates to a monoclonal antibody capable of binding to biotin. In one embodiment the monoclonal antibody according to the invention also does not bind to a biotin moiety on a biotinylated molecule, wherein the biotin moiety is attached to the molecule via the carbon atom of the carboxyl function of the valeric acid moiety of biotin. Also disclosed is a method for generation of an antibody as disclosed herein. The monoclonal antibody according to the invention is of specific use in a method for measuring an analyte in a sample, wherein a (strept)avidin / biotin pair is used to bind a biotinylated analyte specific binding agent to a (strept)avidin coated solid phase.

Biotin-containing monomers, polymeric materials formed from the biotin-containing monomers, articles containing the polymeric materials, methods of making the articles, and methods of using the articles are provided. The articles can be used, for example, for affinity capture of biotin-binding proteins, including biotin-binding fusion proteins (i.e., a biotin-binding protein fused to another biomaterial). Articles that contain captured biotin-binding proteins can be further used for affinity capture of various biotin-containing biomaterials such as biotinylated proteins. The articles can also be used, for example, for affinity capture of biotin-binding fusion proteins where the fusion protein includes, for example, an enzyme or antibody.

The present invention relates to a monoclonal antibody capable of binding to biotin. In one embodiment the monoclonal antibody according to the invention also does not bind to a biotin moiety on a biotinylated molecule, wherein the biotin moiety is attached to the molecule via the carbon atom of the carboxyl function of the valeric acid moiety of biotin. Also disclosed is a method for generation of an antibody as disclosed herein. The monoclonal antibody according to the invention is of specific use in a method for measuring an analyte in a sample, wherein a (strept)avidin / biotin pair is used to bind a biotinylated analyte specific binding agent to a (strept)avidin coated solid phase.

The present invention relates to a gene construct characterized by encoding a fusion protein of flgM and Strepavidin having biotin binding ability through the linkage of an anti-sigma factor flgM gene and a gene encoding streptavidin, which enables expression and secretion of a streptavidin-fusion protein with biotin binding ability in an anti-cancer strain capable of targeting tumor sites and stimulating immune activity, and to an anti-cancer adjuvant and tumor imaging agent that, when transformed by this construct, exhibits anti-cancer activity itself and can be effectively utilized in the diagnosis and treatment of tumor cells together with anti-cancer substances or contrast agents labeled with biotin, which specifically interacts with streptavidin.

Technologies for the prevention and / or treatment of pathogenic or microbial infections. Compositions relate to compositions, vaccines and methods comprising an immunogenic multiple antigen presenting system (MAPS), where two or more biotinylated polysaccharide antigens are interconnected or joined together by one or more fusion proteins comprising (i) a sialic acid binding domain (SBD) and (ii) a biotin-binding moiety (BBM), thereby facilitating the joining multiple polysaccharide antigens together in the complex to form a cross-linked MAPS-X immunogenic complex. The polysaccharide antigens that are linked can be on the same polysaccharide macromolecule or on distinct polysaccharide macromolecules.