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Construction method of enzyme-reponsive multifunctional nano-coating

A nano-coating and construction method technology, applied in coatings, pharmaceutical formulations, surgery, etc., can solve the problems of surface modification of nano-coating cardiovascular materials, loss of biological activity, and difficulty in maintaining long-term functions of biological coatings. , to achieve the effect that the construction process and fixing method are simple and easy to operate, realize functional modification, and have a wide range of applications.

Active Publication Date: 2017-11-24
HUAIYIN INSTITUTE OF TECHNOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the complex environment of the body, many biological coatings are difficult to maintain long-term functions, but rapidly lose their biological activity in the dynamic environment of the body.
At present, there is no relevant report on the surface modification of this enzyme-responsive nano-coating cardiovascular material.

Method used

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  • Construction method of enzyme-reponsive multifunctional nano-coating
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  • Construction method of enzyme-reponsive multifunctional nano-coating

Examples

Experimental program
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Effect test

Embodiment 1

[0032] see figure 1 , the first specific embodiment of the present invention is a method for constructing an enzyme-responsive nano-coating, the steps of which are:

[0033] A) After the surface of 316L stainless steel was polished and cleaned, it was immersed in a dopamine solution with a concentration of 2 mg / ml to react for 12 hours. After ultrasonic cleaning with double distilled water, the above steps were repeated twice to obtain three layers of polydopamine coating surface, dry at 37°C;

[0034] B) Soak the sample deposited with polydopamine coating in step A) in 0.1 mg / ml avidin solution, let it stand for reaction at 37°C for 8 hours, then wash the sample with double distilled water, and save it for later use;

[0035]C) The biotinylated heparin sodium solution with a concentration of 10 mg / ml and the polyethyleneimine solution with a concentration of 1 mg / ml were blended in equal volumes, and ultrasonically treated for 5 minutes at room temperature to obtain a nanopa...

Embodiment 2

[0043] A method for constructing an enzyme-responsive nano-coating, the steps of which are:

[0044] A) After the surface of 316L stainless steel was polished and cleaned, it was immersed in a dopamine solution with a concentration of 2 mg / ml to react for 12 hours. After ultrasonic cleaning with double distilled water, the above steps were repeated twice to obtain three layers of polydopamine coating surface, dry at 37°C;

[0045] B) Soak the sample deposited with polydopamine coating in step A) in 0.5 mg / ml avidin solution, let it stand for reaction at 37°C for 8-24 hours, then wash the sample with double distilled water, and store it until use;

[0046] C) The biotinylated heparin sodium solution with a concentration of 20 mg / ml and the polyethyleneimine solution with a concentration of 2 mg / ml were blended in equal volumes, and ultrasonically treated for 5 minutes at room temperature to obtain a nanoparticle suspension. Submerge the sample obtained in step B into the nano...

Embodiment 3

[0054] A method for constructing an enzyme-responsive nano-coating, the steps of which are:

[0055] A) After the surface of 316L stainless steel was polished and cleaned, it was immersed in a dopamine solution with a concentration of 2 mg / ml to react for 12 hours. After ultrasonic cleaning with double distilled water, the above steps were repeated twice to obtain three layers of polydopamine coating surface, dry at 37°C;

[0056] B) Soak the sample deposited with polydopamine coating in step A) in 0.3 mg / ml avidin solution, let it stand for reaction at 37°C for 12 hours, then wash the sample with double distilled water, and save it for later use;

[0057] C) The biotinylated heparin sodium solution with a concentration of 15 mg / ml and the polyethyleneimine solution with a concentration of 1.5 mg / ml were blended in equal volumes, and ultrasonically treated for 5 minutes at room temperature to obtain a nanoparticle suspension. Submerge the sample obtained in step B into the na...

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Abstract

The invention discloses a construction method of an enzyme-reponsive multifunctional nano-coating. The construction method comprises the following steps: firstly, immobilizing avidin molecules to the surface of dopamine-coated stainless steel; then, on the basis of specific recognition and binding effects between avidin and biotin, immobilizing biotinylated heparin / PEI nanoparticles to the surface of a material, and further binding high-concentration avidin molecules to residual biotin on the surface of a nanoparticle coating, so that a novel biotin binding site is introduced; then, continuing to assemble biotinylated enzyme-responsive polypeptide to the surfaces of the nanoparticles on the basis of an interaction between the biotin and the avidin; and finally, by virtue of an EDC / NHS / MES coupling agent, immobilizing SDF-1[alpha] to an amino terminal of the polypeptide in a covalent mode, so that the multifunctional nano-coating, which has a matrix metalloproteinase 9 responsive characteristic, is constructed. According to the construction method provided by the invention, by constructing a multifunctional layer, which has anti-coagulating and endothelial regeneration inducing capacities, on a titanium surface, blood compatibility and a damaged endothelial repair capacity of the material can be obviously improved.

Description

Technical field [0001] The invention relates to the technical field of surface modification technology of inorganic materials, in particular to a method for constructing an enzyme-responsive multifunctional nano-coating. Background technique [0002] Cardiovascular diseases such as coronary heart disease seriously threaten human life and health. At present, clinically, metal vascular stent intervention is mainly used to improve the blood supply of patients with coronary heart disease. However, due to insufficient biocompatibility of traditional bare metal stents and drug-eluting stents, they often cause mid-to-late thrombosis and vascular intimal hyperplasia after implantation, leading to implant failure and even endangering the safety of patients. [0003] By biochemically modifying the surface of materials, endowing materials with good anticoagulant ability and inducing endothelial regeneration ability is a commonly used method to improve the biocompatibility of materials...

Claims

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Application Information

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IPC IPC(8): A61L31/10A61L31/16A61L31/14
CPCA61L31/10A61L31/14A61L31/16A61L2300/236A61L2300/252A61L2300/412A61L2300/42A61L2300/602A61L2300/624A61L2400/12A61L2400/18A61L2420/06A61L2420/08C08L79/02C08L89/00C08L5/10
Inventor 刘涛罗逊刘诗卉龚韬汤潇涵潘长江丁红燕
Owner HUAIYIN INSTITUTE OF TECHNOLOGY
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