37 results about "Amino terminal" patented technology

The invention discloses decapeptide with antioxidant activity, the amino acid sequence of the decapeptide is amino terminal-APKTLPWGPK-carboxyl terminal, and the decapeptide can be developed into an antioxidant.

The application belongs to the technical field of biological detection, and discloses a cardiovascular marker control article and a preparation method thereof. The cardiovascular marker control article comprises a serum matrix, antigens, a preservative and a stabilizer. The antigens include cardiac troponin I antigens, creatine kinase isoenzyme antigens, myoglobin antigens, amino-terminal brain natriuretic peptide precursor antigens, brain natriuretic peptide antigens, D-dimer antigens, heart-type fatty acid-binding protein antigens, myeloperoxidase antigens and lipoprotein phospholipase A2 antigens. The stabilizer includes at least one of D-trehalose, sucrose, glycine, inositol and polyethylene glycol. The cardiovascular marker control article can cover various cardiovascular disease markers, including heart failure, pulmonary embolism, vasculitis and atherosclerosis, myocardial injury, myocardial infarction and the like. The control article can be used as a third-party control article for indoor and inter-laboratory quality control of a cardiovascular disease screening and auxiliary diagnosis measurement system.

Activatable interleukin 12 (IL12) fusion proteins and constructs are disclosed herein that contain at least one of the following structures: N'-DD-L1-MM-L2-IL12-C', C'-DD-L1-MM-L2-IL12-N', N'-DD-L1-IL12-L2-MM-C', C'-DD-L1-IL12-L2-MM-N', wherein L1 is a first linker or is absent; L2 is a second linker; DD is a dimerization domain; MM is a masking moiety; IL12 comprises an IL12α (p35) domain and an IL12β (p40) domain; N' is an amino terminus of the activatable IL12 fusion protein; and C' is a carboxy terminus of the activatable IL12 fusion protein, wherein at least one of L1 and L2 comprises a cleavable moiety (CM); or a first IL12 subunit and a second IL12 subunit; at least one of a first linking region (LR1) and a second linking region (LR2); and a half-life extending moiety (EM), wherein at least one of the LR1 and the LR2 comprises a cleavable moiety (CM); or dimers thereof.

The invention provides a whey protein isolate source antioxidant polypeptide, the amino acid sequence of the whey protein isolate source antioxidant polypeptide is amino terminal-LVYPFPGPIP-carboxyl terminal, and the whey protein isolate source antioxidant polypeptide can be developed into an antioxidant.

This disclosure generally relates to fusion proteins comprising TAL Arrays targeting a repetitive element and a transposase domain comprising amino terminal deletions, as well as dual cysteine rich domains (CRD), for targeting site-specific transposition into ribosomal DNA (rDNA) repeats or LINE1 repetitive elements, polynucleotides and vectors encoding the fusion proteins, and methods of use therefor.

The present application belongs to the technical field of organic framework materials, and particularly relates to a kind of covalent organic framework material and its preparation method and application.The present application covalent organic framework material includes electron donor and electron acceptor;Electron donor and electron acceptor are connected by covalent bond;Electron donor includes organic ligand containing aldehyde group terminal group;Electron acceptor includes organic ligand containing amino terminal group.The present application is constructed by specific kind of electron donor-electron acceptor structure, so that the redox center in the covalent organic framework material is separated in space, which is beneficial to the rapid separation and migration of photo-generated carriers and improves the lifetime of photo-generated carriers, thereby efficient photocatalysis-piezoelectricity synergistic catalytic reaction is generated H2O2, and the yield of hydrogen peroxide can be obviously improved, and under the synergistic effect of photocatalysis-piezoelectricity, the efficiency of hydrogen peroxide produced by the material is higher than that under the condition of photocatalysis.

The invention discloses a method for preparing semeglutide by using a natural chemical ligation method, which comprises the following steps: (1) synthesizing semeglutide in two segments, namely an N-terminal segment with carboxyl-terminal hydrazide and a C-terminal segment with amino-terminal cysteine; (2) connecting the two fragments through a natural chemical connection method under a buffer salt condition; and (3) performing desulfurization treatment to convert Cys into target amino acid, and performing purification and freeze-drying after complete treatment. According to the method, the semeglutide is divided into the C-terminal polypeptide fragment and the N-terminal polypeptide fragment, the C-terminal polypeptide fragment and the N-terminal polypeptide fragment are connected through natural chemical connection reaction after being prepared respectively, then desulfurization treatment is conducted, the high-purity and high-yield semeglutide is finally obtained, the problem of difficult sequences is solved, the synthesis efficiency and purity of the semeglutide are remarkably improved, and the method is suitable for industrial production. The method is suitable for large-scale production.

The invention discloses a whey protein source peptide with hyaluronidase inhibitory activity. The whey protein source peptide is characterized in that the sequence of the whey protein source peptide is amino terminal-GGVSLPEW-carboxyl terminal. The peptide has the advantages of no toxicity, no sensitization and the like, has a remarkable inhibition effect on hyaluronidase, and can be used for developing functional foods or medicines with anti-allergic characteristics.

The invention provides a biosensor for detecting nitrate content as well as a preparation method and application thereof, and relates to the technical field of biology. The biosensor disclosed by the invention comprises a luciferase amino terminal fragment, a luciferase carboxyl terminal fragment and NasR protein, the luciferase amino terminal fragment and the luciferase carboxyl terminal fragment are two fragments of the same luciferase. The biosensor provided by the invention has high selectivity on nitrate, can specifically, stably, continuously and online observe the nitrate content in the whole plant for a long time, and overcomes the key problems of destructive sampling, limited spatial resolution, insufficient detection sensitivity and the like in the traditional method; and a real-time dynamic detection platform capable of monitoring the whole plant living body is provided for plant nitrogen metabolism research.

The present invention relates to the biomedical and biopharmaceutical sectors. Specifically, it relates to a chimeric antigen comprising the combination of the amino- and carboxy-terminal regions of the amyloid-beta peptide (Aβ), the amino- and carboxy-terminal regions of the tau protein, and a T-cell epitope. The pharmaceutical composition comprising this chimeric antigen and at least one pharmaceutically acceptable vaccine adjuvant increases the efficacy of immunotherapy for the prevention and treatment of Alzheimer's disease (AD). The chimeric antigen exerts its action by stimulating a multi-target humoral response with high titers of anti-Aβ and anti-tau antibodies simultaneously. This promotes the combined elimination of toxic Aβ and tau species from the brain, thereby preventing or significantly improving the clinical symptoms and neuropathology of AD.

To provide an antitumor antagonist comprising a first targeting domain comprising a mutated TGFβ1-R11 extracellular domain (mutated ECD); and an immunoglobulin scaffold having an amino terminus and a carboxy terminus.SOLUTION: The present disclosure relates to an antitumor antagonist comprising a first targeting domain comprising a mutated TGFβ1-R11 extracellular domain (mutated ECD); and an immunoglobulin scaffold having an amino terminus and a carboxy terminus, the one or more mutations reducing proteolytic cleavage of the antitumor antagonist. Also disclosed are methods for treating proliferative disorders, infections, and immunological disorders with the antitumor antagonists described herein.SELECTED DRAWING: Figure 20

The application provides a preparation method of dopamine (DA) modified polyamide-amine dendrimer, which comprises amino terminal dopamine carboxylation, synthesis of carboxylated dopamine with a protective group, synthesis of carboxylated dopamine with a protective group modified polyamide-amine dendrimer, and synthesis and application of dopamine modified polyamide-amine dendrimer. The application utilizes the firm adhesion performance of DA in a marine humid environment, the antibacterial performance and the osteogenic induction performance of PAMAM-NH2 cationic polymer, introduces DA into the surface of PAMAM-NH2 to synthesize a new high polymer material DA-PAMAM-NH2, detects the long-acting antibacterial and osteogenic induction performance of the system as a bifunctional coating on the surface of an implant, and evaluates the role of the system in improving the clinical durability of the implant.

The invention discloses a whey protein source peptide with hyaluronidase inhibitory activity. The whey protein source peptide is characterized in that the sequence of the whey protein source peptide is amino terminal-GVSLPEW-carboxyl terminal. The peptide has the advantages of no toxicity, no sensitization and the like, has a remarkable inhibition effect on hyaluronidase, and can be used for developing functional foods or medicines with anti-allergic characteristics.

The present invention relates to a fusion peptide, a salt thereof, or a solvate thereof, the fusion peptide containing, from the amino terminus toward the carboxy terminus, a tag peptide and any peptide among (A1)-(A3). (A1) A peptide that comprises the amino acid sequence represented by SEQ ID NO: 1; (A2) a peptide that comprises an amino acid sequence obtained by deleting, substituting, or adding 1-4 amino acids in the amino acid sequence represented by SEQ ID NO: 1 and that has a pore density increasing effect; and (A3) a peptide that comprises an amino acid sequence having 90% or more sequence identity with the amino acid sequence represented by SEQ ID NO: 1 and that has a pore density increasing effect.

The present application belongs to the technical field of protein engineering and genetic engineering, and particularly relates to a recombinant collagen XVII and a preparation method and application thereof. Specifically, a brand new recombinant collagen is designed through screening, replacement and splicing, the nucleotide sequence of the recombinant collagen is optimized according to the codon bias of Pichia pastoris, a transmembrane peptide is introduced into the amino terminal of the sequence, a corresponding genetic engineering yeast strain is constructed, and the recombinant collagen is successfully prepared through fermentation. The amino acid sequence of the recombinant collagen has a homology of 100% with the corresponding region of natural human collagen XVII, and there is no immunogenicity risk caused by sequence difference. Meanwhile, the endotoxin content of the protein can be controlled through a purification process, so that no endotoxin residue is ensured. The recombinant collagen prepared by the above technical scheme has more excellent transdermal performance and cell proliferation activity, and has better stability, and therefore has good practical application value.

The invention belongs to the technical field of protein engineering and genetic engineering, and particularly relates to a recombinant XVII type collagen as well as a preparation method and application thereof. Specifically, brand new recombinant collagen is designed by means of screening, replacement, splicing and the like, pichia pastoris codon preference optimization is performed on a nucleotide sequence of the recombinant collagen, cell-penetrating peptide is introduced to an amino terminal of the sequence, a corresponding genetic engineering yeast strain is constructed and obtained, and the recombinant collagen is successfully prepared by fermentation. The homology of the amino acid sequence of the gene and the corresponding region of the natural human source XVII type collagen reaches 100%, and the immunogenicity risk caused by sequence difference does not exist; meanwhile, the endotoxin content of the protein can be controlled through a purification process, and no endotoxin residue is ensured. The recombinant collagen prepared by the technical scheme has more excellent transdermal performance and cell proliferation activity, and also has better stability, so that the recombinant collagen has good practical application value.

By deleting a nucleotide sequence encoding the amino terminal region and a nucleotide sequence encoding the carboxy terminal region of the E120R protein in the genome, the modified African swine fever virus does not remain in the body of the inoculated animal and exhibits a vaccine effect against African swine fever.

The invention provides single-molecular-weight oligoproline with thermal responsiveness as well as a preparation method and application thereof, and belongs to the technical field of biological materials and high polymer chemistry. The preparation method comprises the following steps: synthesizing an oligoproline-solid-phase carrier compound with a protected amino terminal by adopting a solid-phase synthesis method; after an amino terminal protecting group is removed, carboxylic acid containing a hydrophobic group reacts with the amino terminal protecting group to realize hydrophobic modification of the amino terminal; and finally, cutting and purifying to obtain the thermal-responsive single-molecular-weight oligomeric proline of which the amino terminal is modified by a hydrophobic group. According to the invention, C2-C8 alkyl is introduced into the terminal amino group of oligoproline, so that oligoproline with low molecular weight and single molecular weight is successfully endowed with thermal responsiveness, the product structure is clear, the phase change behavior is clear, an ideal model is provided for design of a temperature-sensitive material and research on a phase separation mechanism, and the application prospect is wide. And the application of oligoproline in the fields of biological materials and drug delivery is expanded.

This disclosure generally relates to fusion proteins comprising TAL Arrays targeting a repetitive element and a transposase domain comprising amino terminal deletions, as well as dual cysteine rich domains (CRD), and methods of use therefor.

This disclosure generally relates to transposase domains, in particular, transposase domains comprising amino terminal deletions, as well as transposase domains forming obligate heterodimers and transposase domains comprising DNA targeting domains.

Disclosed are a BAFFR-targeting chimeric antigen receptor, a CAR-T cell, and a use. The BAFFR-targeting chimeric antigen receptor comprises a signal peptide region, a BAFFR-targeting antigen binding domain, a hinge region, a transmembrane domain, a costimulatory domain, and a signal transduction domain, which are connected in order from the amino terminal to the carboxyl terminal, wherein the costimulatory domain comprises a CD28 costimulatory domain and a 4-1BB costimulatory domain which are connected, and the signal transduction domain is ITAM1 of CD3ζ. The CAR-T cell is constructed by means of BAFFR. By utilizing an antigen-antibody binding mechanism, autoimmune B cells or malignant proliferating B cells in patients with autoimmune diseases or some tumors can be effectively consumed, providing a new choice for the treatment of human autoimmune related diseases and some hematologic tumors.

The invention discloses a cell line for stably expressing porcine delta coronavirus S protein, a porcine delta coronavirus IgA antibody detection antigen and a kit. The method comprises the following steps: based on an S protein sequence of a CH / XJYN / 2016 strain, selecting an extracellular domain part D20-N1077, adding a human source septoglobin family 1D meber 1 signal peptide sequence MRLSVCLLLTLALCCYRANA to an amino terminal of the S protein, adding a 6 * His tag sequence to a carboxyl terminal, carrying out codon optimization according to a CHO expression system, and constructing into a pCDNA3.1 (+) eukaryotic expression vector to obtain a recombinant eukaryotic expression plasmid pcDNA3.1 (+)-PDCoV-S; then transfecting CHO cells with the plasmid, and carrying out two rounds of heredity mycin G418 pressurized screening, limited dilution cloning and continuous subculture to obtain an expiCHO-PDCoV-S stably transfected cell line through screening; and finally, constructing the PDCoV IgA antibody detection kit based on the cell line. The kit has no cross reaction with other common porcine disease virus positive serum, has intra-batch and inter-batch variation coefficients of less than 10%, has good specificity, repeatability and sensitivity, and can be used for early prevention and control of PDCoV.

The invention relates to the technical field of biological medicines, and particularly discloses application of an adeno-associated virus vector in preparation of a medicine for treating ovarian cancer. The adeno-associated virus vector comprises a modified AAV2 capsid and polynucleotide which is loaded in the modified AAV2 capsid and carries a therapeutic gene; wherein the EC1 targeting peptide is inserted into the amino terminal of the VP2 protein of the AAV2 capsid, and the VP1 protein has R585A and R588A mutations. Compared with a conventional virus vector, the vector can specifically target the ovarian cancer cells, efficient infection is achieved, the infection capacity of the vector on normal tissues such as the liver is remarkably reduced, and the vector has the advantage in the aspect of safety. In-vivo experiments show that by combining caudal vein injection of the adeno-associated virus vector carrying the HSV-TK with ganciclovir, tumor proliferation of mice with ovarian cancer transplanted tumors can be remarkably inhibited, and tissue organs such as liver and kidney of the mice are not obviously damaged.

The application discloses a recombinant Bst DNA polymerase with high DNA polymerase activity and reverse transcriptase activity, and preparation and application thereof. Sulfolobus tokodaii The application carries out sequence rearrangement on a Headpiece domain truncated body Hp47 derived from chicken villin and a Sto7d domain derived from a thermophilic archaea, and fuses the amino terminal end of the Bst DNA polymerase large fragment to construct a novel Bst DNA polymerase rearrangement mutant HpSt-G23. The recombinant polymerase HpSt-G23 obtained by the application has high polymerase activity and reverse transcriptase activity, and can resist inhibitors such as urea and sodium chloride. Tth The HpSt-G23 can be used in a RecA detection system, and only two primers are needed to complete DNA or RNA amplification in the same system, and the detection sensitivity of the RNA template is greatly improved, so that the HpSt-G23 has a good development prospect in the field of nucleic acid detection.

The invention discloses a tetradecapeptide with antioxidant activity, the amino acid sequence of the tetradecapeptide is amino terminal-MFLSFPTTKTYFPH-carboxyl terminal, and the tetradecapeptide can be developed into an antioxidant.

The invention discloses a polymerase labeled mouse and rabbit universal secondary antibody as well as a preparation method and application thereof. The polymerase labeled mouse and rabbit universal secondary antibody comprises an anti-mouse IgG and rabbit IgG recombinant antibody, and the anti-mouse IgG and rabbit IgG recombinant antibody sequentially comprises an anti-mouse IgG nano antibody, a connecting peptide, an anti-rabbit IgG nano antibody, a human IgG hinge region peptide fragment, a polylysine peptide fragment and a purification label from an amino terminal to a carboxyl terminal. According to the present invention, the anti-mouse IgG and anti-rabbit IgG recombinant antibody with the brand new structure is designed to obtain the recombinant antibody with the multiple primary amine groups at the carboxyl terminal, and the primary amine groups can be further utilized to couple the labeled enzyme so as to develop the preparation method of the polymerase-labeled mouse-rabbit universal secondary antibody, such that the polymerase-labeled mouse-rabbit universal secondary antibody can be obtained; the secondary antibody signal amplification effect can be improved, the secondary antibody preparation period is shortened, the secondary antibody preparation process is simplified, the stability between batches is improved, and meanwhile dependence on experimental animals is reduced.

The present application relates to the technical field of single molecule device, and especially relates to a single molecule device for detecting small molecule gas based on supramolecule and a preparation method thereof. The single molecule device comprises a pair of graphene electrodes and a functional molecule, and the functional molecule is connected between the pair of graphene electrodes through an amide bond. A cucurbituril hydrophobic cavity structure in a structural formula of the functional molecule can accommodate a target small molecule gas, selectively encapsulate gas molecules of different volumes through multiple weak interactions, form a dynamic controllable host-guest complex structure, and then realize "on-off type" sensing and detection of the small molecule gas. Amino terminals at end portions of a host bridging unit in the functional molecule can be connected with carboxyl terminals of the graphene point electrodes through a covalent bond, and a molecular chain connected through amide condensation is formed at both ends of the graphene point electrodes, so that the stability of the single molecule device is improved. The preparation method provided by the present application is simple in process, mild in reaction condition, controllable, and beneficial to large-scale production.

The invention relates to the field of biological medicines and daily chemicals, and discloses a bioactive peptide and a derivative thereof. The amino acid sequence of the bioactive peptide is FKTT; and modifying the amino terminal, carboxyl terminal or any amino acid side chain group to obtain the derivative. The bioactive peptide and the derivative thereof have good anti-aging and anti-oxidation effects and the like, can be widely applied to the fields of medicines, daily chemical products, health care products or foods and the like, and have good application prospects.

The invention relates to a cat hepatitis virus core antigen virus-like particle as well as a preparation method and application thereof. The virus-like particle is prepared by expressing a wild type gene DCHcWT of a cat hepatitis virus core antigen or a 1-149 amino acid truncated gene DCHcN149 of an amino terminal of the wild type gene DCHcWT in an insect baculovirus expression system, and purifying and self-assembling the wild type gene DCHcWT or the 1-149 amino acid truncated gene DCHcN149 in the insect baculovirus expression system. The prepared virus-like particles are regular in structure and good in immunogenicity, and antigen characteristics of natural virus particles can be efficiently simulated. The virus-like particle can be used as a diagnosis antigen of cat hepatitis B virus infection, and is used for preparing a serum detection kit for cat hepatitis. The vaccine can also be used as an immunogen to prepare a subunit vaccine for preventing cat hepatitis B; the compound can also be used for vaccine nano-delivery carriers.

The invention provides for a fusion protein comprising a 3E10 Fv joined to a Hsp-70, Hsp-27, Hsp-90 or GRP-78 or portion thereof, and optionally, the 3E10 Fv comprising an amino acid sequence AGIH at its amino terminus.