144 results about "Protein purification" patented technology

The invention discloses a glycosyl transferase mutant and an application of the glycosyl transferase mutant in synthesis of rebaudioside. According to the invention, single-point mutation and multi-point mutation are carried out on the basis of a glycosyl transferase amino acid sequence as shown in SEQ ID NO: 1, and a mutant with improved catalytic activity is obtained. The catalytic activity, the substrate specificity and / or the substrate specificity of the glycosyl transferase mutant are / is changed, and the catalytic activity of enzyme to a specific substrate can be remarkably improved by mutation at a specific site. And carrying out induced expression and protein purification on the obtained mutation sequence to obtain the mutant enzyme. The mutant enzyme is used as a catalyst, and UDPG is used as a glycosyl donor, so that the catalytic reaction efficiency of substrates such as stevioside ST, rebaudioside A (RebA) and rebaudioside D (RebD) can be obviously improved. According to the glycosyl transferase UGT76G1 mutant constructed by the research, the catalytic activity of the glycosyl transferase UGT76G1 mutant is improved, and efficient production of rebaudioside M is realized by optimizing a reaction system.

The invention discloses an AI-assisted protein purification result analysis method and system. The method comprises the following steps: S1, collecting chromatogram data, electrophoresis image data and mass spectrum peak table data; s2, performing data preprocessing after the chromatogram data, the electrophoresis image data and the mass spectrum peak table data are obtained; s3, carrying out AI identification analysis on the preprocessed data to realize electrophoretic band identification, chromatographic peak identification and mass spectrum deconvolution; s4, performing multi-modal fusion on the chromatography, gel electrophoresis and mass spectrum information sources; and S5, automatically generating a report, and producing a visual chart. The deep learning and multi-modal data fusion technology is introduced, comprehensive automatic analysis of chromatographic data, electrophoresis images and mass spectrum results is achieved, then automatic result analysis of the protein purification process is achieved, an artificial intelligence algorithm is used for recognizing a peak structure and an electrophoresis band, multi-modal data fusion is achieved, and the detection accuracy is improved. And generating a visual analysis report according to a data result.

The invention discloses a construction method and application of a chlamydomonas reinhardtii-sourced high-sweetness protein variant engineering algal strain, the construction method comprises the following steps: carrying out gene modification on sweeteners Thaumatin and Brazzein, optimizing preference codons of chlamydomonas reinhardtii to synthesize CrThaumatin and CrBrazzein encoding genes, cloning the encoding genes into an expression vector pGM6, introducing the recombinant expression vector into a chlamydomonas reinhardtii wild-type chlamydomonas sp. Algal strain, and carrying out high-sweetness protein variant engineering algal strain of the chlamydomonas reinhardtii-sourced high-sweetness protein variant engineering algal strain of the chlamydomonas reinhardtii. And screening by using a paromomycin resistance marker carried by the expression vector to successfully obtain an engineering algal strain. According to the present invention, the engineering strain is subjected to fermentation production to obtain the dry and stable Chlamydomonas reinhardtii powder rich in the target sweet protein, and the protein purification oral test results show that the sweet taste of the Chlamydomonas reinhardtii powder is 3-5 times of the sweet taste of the natural Thaumatin and Brazzein protein;

The instant invention relates to compositions comprising a protein, e.g., an antibody, or antigen-binding portion thereof, e.g., an anti-IL-1R1 antibody or antigen binding portion thereof, and methods, e.g., cell culture and protein purification methods, for producing such compositions.

The utility model relates to a protein high-flux purification device in the technical field of protein purification, which comprises a liquid receiving box, a deep hole plate is sleeved at the upper end of the liquid receiving box, a plurality of deep grooves are arranged at the upper end of the deep hole plate, water outlet holes communicated with the liquid receiving box are arranged on the inner bottom walls of the deep grooves, and a gasket is inserted in the deep hole plate. A pair of rectangular blocks are fixedly connected to a pair of side ends of the deep hole plate respectively; after purification processing, a spray head enters a deep groove, and water is sprayed out of the spray head under the pressure of a rubber plate to flush the inner wall of the deep groove, so that during use, the cleaning steps are simple and time-saving, personnel operation is facilitated, and after cleaning, clean water is sucked into a cover plate to be stored for next cleaning and use; and the deep groove is sealed through the cover plate, so that the interior of the deep groove is not prone to being polluted by dust, and dustproof protection is conducted on the deep groove.

To provide a new method for efficiently purifying an exogenous protein expressed in a plant.SOLUTION: A method for purifying a foreign protein expressed in a plant, comprising the step of adding an acid to an extract of a tissue of a plant in which a foreign protein is expressed to degrade contaminating proteins derived from the plant.SELECTED DRAWING: None

The invention provides a process of purification of antibody or fusion protein from protein mixture comprising product and process related impurities. The process provides the use of hydroxyapatite chromatography for the separation of low molecular weight impurities and basic variants. In addition, invention further provides a scalable purification process to remove product and process related impurities.

1. Name of the product in this design: Fully Automated Protein Purification Instrument. 2. Application of this design: For fully automated and efficient purification of various proteins. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

Provided is a technology for improving the efficiency of separating a complex of a protein to be purified and a carrier in a hydrocyclone of a device including an in-line mixer and the hydrocyclone. A protein purification device disclosed herein comprises a first supply unit, a second supply unit, an in-line mixer, a hydrocyclone, and a dilution line. The first supply unit supplies a first slurry containing a carrier including a ligand that binds to a protein. The second supply unit supplies a second slurry which is a cell culture solution containing a protein. The in-line mixer mixes the first slurry and the second slurry. The hydrocyclone separates, downstream of the in-line mixer, a complex of the protein and the carrier from a mixed slurry of the first slurry and the second slurry. The dilution line is provided between the in-line mixer and the hydrocyclone and introduces a diluted liquid into the mixed slurry.

The invention relates to a magnetic bead mediated protein purification device and a high-purity extraction method, and belongs to the technical field of protein purification. The magnetic bead mediated protein purification device comprises a bottom seat, the upper end of the bottom seat is fixedly connected with a circular shell, and the inner bottom of the circular shell is fixedly connected with a bottom magnet; the purification mechanism is mounted on the inner bottom wall of the circular shell; wherein the purification mechanism comprises a plurality of magnetization assemblies mounted on the inner wall of the bottom seat; according to the magnetic bead mediated protein purification device and the high-purity extraction method, the device drives the liquid and the magnetic beads to be mixed through the arranged magnetizing assembly, and after mixing is completed, relatively uniform magnetic attraction can be formed on the liquid storage pipe through the magnetizing assembly, so that the magnetic beads can be uniformly distributed on the inner wall of the liquid storage pipe, and the magnetic beads are uniformly separated from the liquid storage pipe; the occurrence of magnetic bead breakage is reduced, and the extraction effect of protein purification is improved.

1. The name of the design product: protein purification instrument. 2. The use of the design product: biological reaction instrument for protein chromatography separation. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

The invention belongs to the technical field of protein purification, and particularly relates to a chromatography medium, a preparation method of the chromatography medium and application of the chromatography medium to extraction of alpha-lactalbumin. The chromatography medium provided by the invention takes agarose as a matrix, and the surface of the chromatography medium is modified by a ligand through a glucan spacer arm; wherein the molecular weight of the glucan is 20 kDa to 500 kDa, the ligand comprises propylene diamine, and the density of the ligand in the chromatography medium is 200 [mu] mol / mL to 300 [mu] mol / mL. The invention also provides a preparation method of the material, and the material can be obtained by sequentially carrying out epoxy activation, glucan modification, secondary activation and ligand bonding on the matrix. When the chromatography medium provided by the invention is used for extracting alpha-lactalbumin from whey, the chromatography medium has the advantages of high loading capacity, high loading flow rate and efficient regeneration, and meets the requirements of industrial production.

The invention provides a full-automatic detection device and method for the concentration of free drugs in blood. The full-automatic detection method adopts a full-automatic detection device and comprises the following steps: (1) loading a blood sample to a protein purification chromatographic column by adopting a pump 1 and an automatic sampler, removing protein components in the blood sample, and retaining free drug components on the protein purification chromatographic column; (2) enabling free drug components in the protein purification chromatographic column to flow into a trapping column and enriching the free drug components in the trapping column; and (3) enabling the free drug components in the trapping column to flow into an analytical column, separating, and detecting in a triple quadrupole mass spectrometer. Through the specific full-automatic detection device, the problems that total drug concentration monitoring cannot meet clinical treatment requirements, and the free drug pretreatment process is complex, long in time and high in cost are solved, clinical individualized drug administration can be assisted, the drug curative effect is improved, and the toxic and side effects of drugs are reduced.

The utility model relates to the technical field of protein purification, and particularly discloses a portable low-temperature protein purification box which comprises a box body, a control panel is mounted at the top of the box body, a temperature sensor is mounted on the inner wall of the box body, and a sample loading module, a purification module, a detection module and a sample collection module are arranged in the box body. The sample loading module comprises a sample liquid storage device, a buffer liquid storage device and a peristaltic pump which are mounted in the box body, the purification module comprises a chromatographic column connected with the peristaltic pump, and the detection module comprises an ultraviolet detector mounted in the box body. Through the design of the low-temperature control system and the heat preservation layer, protein purification can be carried out in a low-temperature environment, the activity of the protein is effectively maintained, and the problem that the protein is inactivated or even degraded due to the fact that the temperature is too high in a traditional purification method is solved; meanwhile, the influence of long-time low-temperature experiments on the body health of experiment operators is avoided.

In situ Raman spectroscopy methods and systems are provided for the characterization or quantification of a protein purification intermediate and / or the final concentrated fraction during production or preparation. In one embodiment, in situ Raman spectroscopy is used to characterize or quantify critical quality attributes of protein purification intermediates during downstream processing (i.e., after the protein purification intermediate has been obtained). For example, the described in situ Raman spectroscopy methods and systems can be used to characterize and quantify protein purification intermediates as the protein purification intermediates are purified, condensed, or otherwise formulated into the final pharmaceutical product to be marketed or administered.

The application belongs to the technical field of biology, and particularly relates to a SUMO protein, a specific protease thereof and application in protein purification. The amino acid sequences of the SUMO specific protease and the SUMO protein are shown in SEQ ID NO. 1 and SEQ ID NO. 3 respectively. The SUMO protein provided by the application can significantly improve the soluble expression amount of a target protein. More importantly, compared with a commercial scSUMO / Ulp1 system, the SUMO specific protease of the application exhibits higher biochemical robustness in a complex buffer, and can maintain high efficient specific cleavage capacity in a wide temperature range and in a high concentration sodium ion and high concentration imidazole environment. The application overcomes the bottleneck of traditional purification process, i.e. complicated dialysis desalting, and can directly perform in-situ efficient enzymatic cutting on a chromatography eluent, and has great application potential and economic value in industrialized production of recombinant protein purification.

The invention relates to the field of protein purification. The present invention envisages a method for purifying a fermentation-derived solution of a protein of interest, the method comprising the steps of: contacting the fermentation-derived solution with chemically activated carbon in an amount ranging from 0.1 wt% to 15 wt% of the solution; adjusting the pH to a value of 4 to 12, and adjusting the temperature to a value of 2 DEG C to 60 DEG C; separating the chemically activated carbon from the solution; and thereby obtaining a clarified solution of the protein of interest. Furthermore, the invention relates to a clarified solution of a protein of interest obtainable or obtained by the method according to the invention, and the use of chemically activated carbon for purifying a fermentation-derived solution of a protein of interest.

The invention relates to the technical field of biology, in particular to a pagrosomus major antibacterial peptide Pm-NK-lysin as well as a preparation method and application of the pagrosomus major antibacterial peptide Pm-NK-lysin. The amino acid sequence of the antibacterial peptide is as shown in SEQ ID NO: 1. The minimum inhibitory concentration of the antibacterial peptide to vibrio anguillarum is not higher than 8 [mu] g / ml, and / or the minimum inhibitory concentration of the antibacterial peptide to escherichia coli is not higher than 4 [mu] g / ml. The antibacterial peptide is prepared through a pichia pastoris expression system. The antibacterial peptide is applied to preparation of an antibacterial preparation. According to the invention, efficient secretory expression of recombinant Pm-NK-lysin is successfully realized by creatively utilizing a pichia pastoris eukaryotic expression system, and a protein purification process is optimized, so that sufficient sources of high-activity recombinant protein are guaranteed. The Pm-NK-lysin provided by the invention has an excellent killing effect on aquatic pathogenic bacteria. The antibacterial peptide Pm-NK-lysin is used for replacing or reducing the use of antibiotics to control the harm of aquatic bacterial diseases, has wide popularization and application values, and certainly has far-reaching significance for promoting the green and healthy development of the aquaculture industry.

The application belongs to the technical field of protein purification, and particularly relates to a recombinant human metapneumovirus pre-fusion F protein purification method, which comprises the following steps: filtering fermentation broth by using a deep filter to obtain fermentation clarified liquid; capturing the recombinant hMPV F protein from the fermentation clarified liquid by using hydroxyapatite to obtain purified liquid 1; inactivating viruses in the purified liquid 1 by using a low-pH method to obtain inactivated liquid; performing anion exchange chromatography on the inactivated liquid to obtain purified liquid 2; performing composite weak anion chromatography on the purified liquid 2 to obtain purified liquid 3; performing UF / DF on the purified liquid 3 to obtain ultrafiltrate; and performing virus-removing nanofiltration and sterile filtration on the ultrafiltrate to obtain a recombinant hMPV F stock solution. The method is simple, reproducible, suitable for large-scale production, and can be effectively used for producing a recombinant human metapneumovirus pre-fusion F protein vaccine.

The application relates to a recombinant type I collagen and application thereof, which utilizes the characteristics of Pichia pastoris capable of secreting and expressing recombinant proteins, secretes the recombinant type I collagen on the culture medium supernatant, and reduces the difficulty of protein purification. In addition, the integrin site RGD is inserted into the collagen to form a recombinant type I collagen with high stability and high expression. According to the relative proliferation of cells and the relative adhesion experiment of cells, it can be seen that the recombinant type I collagen has the effects of promoting the proliferation of skin fibroblasts, improving the skin, promoting wound healing, promoting the adhesion of fibroblasts in the skin layer and the like. Therefore, the recombinant type I collagen has great potential applications in the fields of skin care and beauty, medical treatment and the like.

The utility model relates to the technical field of protein purification devices, and solves the problems that in the process of using a protein purification instrument, a pipeline needs to continuously extract a solution in a bottle body, a user only needs to simply place the bottle body on the top surface of the protein purification instrument, a structure for fixing the bottle body is lacked, the bottle body is easily influenced by external force and topples over, and the use cost is high. In addition, the problems that the solution intrudes into the protein purification instrument, and the protein purification instrument is damaged are also solved. The protein purification device comprises a protein purification instrument, the top surface of the protein purification instrument is fixedly connected with a plurality of positioning assemblies, and the side surface of the protein purification instrument is fixedly connected with a wire clamp assembly; the positioning assembly comprises stretching damping rods which are fixedly connected to the top surface of the protein purification instrument in a rectangular array manner, the top ends of the stretching damping rods are fixedly connected with positioning blocks, grooves are formed in the side surfaces of the positioning blocks in a rectangular array manner, and rotating blocks are rotationally connected to the interiors of the grooves.

The utility model discloses a continuous feeding device for protein purification, which relates to the technical field of protein purification, and comprises a fixed bottom plate and a feeding component, a support column is mounted on the upper surface of the fixed bottom plate, a support ring is arranged at the top of the support column, and the feeding component is arranged on the inner side of the support ring. And the feeding assembly comprises an auxiliary feeding tank, a servo motor, a feeding auger, a connecting material pipe, a discharging switch valve, a material conveying pipe, a material conveying pump body and a feeding connecting pipe, the auxiliary feeding tank is arranged on the surface of the inner side of the supporting ring and is of an integrated structure, and the servo motor is installed on the right surface of the auxiliary feeding tank. According to the continuous feeding device for protein purification, automatic and continuous feeding can be achieved through the feeding assembly, the conveying amount and conveying time of materials can be adjusted according to needs, the materials can be fully filtered through the filtering assembly, and the subsequent purification efficiency and quality are improved.

To provide a process for purifying an antibody or a fusion protein from a protein mixture containing product - and process-related impurities.SOLUTION: Provided is a process for purifying a protein of interest from a protein mixture comprising the protein of interest and one or more low molecular weight (LMW) variants, comprising: a. loading the protein mixture onto a ceramic hydroxyapatite (CHT) column with a suitable buffer; b. washing the CHT column with a suitable buffer; and c. eluting the purified protein of interest with a suitable gradient of phosphate buffer, wherein the protein of interest is substantially pure and has less than 0.4% LMW as analyzed by SE-HPLC analysis.SELECTED DRAWING: None

The application belongs to the technical field of membrane separation, and particularly relates to a small-aperture high-flux ultrafiltration membrane and a preparation method and application thereof. Polymer material, an additive and an organic solvent are mixed, constant-temperature stirring and standing defoaming are performed to obtain a casting solution, and then a non-solvent phase separation method is used to prepare the ultrafiltration membrane. The polymer material comprises polyethersulfone and sulfonated polyphenylsulfone with a mass ratio of 95:5-80:20, and the additive is polyethylene glycol. The rejection rate of the ultrafiltration membrane to bovine serum albumin (BSA) is above 99%, the rejection rate of direct red 23 and coomassie brilliant blue and other dyes can be above 96%, and the pure water permeability is 310 L·m ‑2 ·h ‑1 ·bar ‑1 , which has a wide application prospect in the fields of antibody protein purification and concentration, dye desalination and nanoscale pollutant treatment in wastewater.

The utility model belongs to the technical field of light-emitting devices, in particular to an ultraviolet lamp stable light-emitting device for an analytical instrument, which comprises an irradiation device and a driving device, the irradiation device comprises a shell, a light-gathering cover is mounted in the shell, three ultraviolet lamp tubes are mounted in the light-gathering cover, the rear end of the shell is fixedly connected with a mounting block, and the mounting block is fixedly connected with the driving device. And the mounting block is fixedly connected with a rotating shaft. According to the utility model, the three ultraviolet lamp tubes in triangular arrangement are matched with the light gathering cover to form a uniform and stable ultraviolet illumination area, so that the detection precision is improved; the bevel gear disc transmission and the driving shaft are matched with the rotating shaft, so that the angle of the irradiation device is accurately adjustable to adapt to different detection scenes; the light-transmitting glass isolates dust and impurities to guarantee the cleanness of a light path; the driving shaft is mounted on the driving box through a bearing seat to ensure stable transmission and low noise; the overall structure gives consideration to light source stability, angle flexibility and environmental adaptability, and the detection efficiency and reliability of an analytical instrument in scenes such as protein purification are remarkably improved.

This invention discloses a method for hydroxylation modification of recombinant mussel foot protein and its application. The invention utilizes a whole-cell catalyst with tyrosinase catalytic activity to hydroxylate tyrosine residues in recombinant mussel foot protein. This method eliminates the need for cell disruption and protein purification steps. By immobilizing tyrosinase on the host cell surface through cell surface display, the recombinant mussel foot protein can directly contact the tyrosinase in the extracellular environment and undergo hydroxylation, thereby introducing dopamine groups into the protein to improve its underwater adhesion properties. This method is applicable to the hydroxylation modification of various types of recombinant mussel foot protein and has advantages such as high catalytic efficiency, good stability, simple operation, and low cost, making it valuable for industrial applications.

The utility model relates to the technical field of protein purification, in particular to a protein purification column convenient to clean, which comprises a column body and a vibration component, the column body is divided into a mounting section and a purification section, a sieve plate is arranged in the purification section, and a filler column is arranged above the sieve plate; a driving cavity is formed in the mounting section, a collision convex block is arranged on the driving cavity, and a vibration assembly is mounted in the driving cavity; the vibration assembly generates vibration by colliding with the collision protruding block. Vibration can be stably generated through the vibration assembly, the vibration frequency is controlled according to the actual situation, the contact efficiency of NaCl and residual protein in the column body is improved, then the cleaning efficiency is improved, cleaning is more convenient, meanwhile, the sieve plate is cleaned through vibration, and the problem that the sieve plate is blocked is solved.

The inventions provide improved systems and methods for inline concentrating and reducing intermediate process pools to mitigate plant fit challenges associated with high-titer protein purification process. The inventions also provide methods for concentrating and reducing VI pool volumes by integrating columns of single pass tangential flow filtration (SPTFF) system with viral inactivation pool filtration (VIPF) in protein purification process. Therapeutic proteins purified by the methods also are provided.

The present application relates to the technical field of protein purification, and particularly relates to a porcine circovirus vaccine protein purification device, which comprises a protein purification instrument, a tray is arranged on the top of the protein purification instrument, and a connecting mechanism is arranged at the connecting position of the tray and the protein purification instrument. The tray is installed and fixed through the mutual insertion of the convex block and the pin rod, so that the tray is more convenient to take down and install and is easy to clean. The internal inclined surface of the tray achieves the effect of flow guiding, liquid is stored in the water collecting box, dripping on the experiment table is avoided, the safety of the experiment is improved, the height adjustment of the bracket is more convenient through the cooperation of the arc-shaped clamping block and the arc-shaped groove, the positioning effect is achieved through the cooperation of the spherical convex and the spherical groove, the adjustment of the installation position of the pre-packed column is more convenient, and the operation is more simple and convenient during the protein purification operation.

The invention relates to the technical field of biology, in particular to a preparation and purification process of a DPEase mutant. According to the present invention, I46E / K86I / K86V / H102P / Y111A / N176P / C213N / M249P / Y285E modification is performed on the D-psicose 3-epimerase DPEase derived from Rumiclostridium cellulolyticum, such that the expression quantity and the enzyme activity of the enzyme in Escherichia coli are improved, and the industrial fermentation process and the industrial protein purification process of the enzyme are optimized, such that the D-psicose 3-epimerase prepared by using the process can be easily produced under the conditions of short time and lowest cost so as to significantly improve the yield and the yield of the enzyme; the conversion rate is the same as or even higher than that in the prior art, and the systematic optimization strategy enables the production efficiency to be higher and is more suitable for large-scale industrial production.