99 results about "Crucian carp" patented technology

The present invention discloses a strain of Stutzerimonas kunmingensis and its application in water denitrification, relating to the field of biotechnology. The Stutzerimonas kunmingensis was deposited in the General Microbiology Center (CGMCC) of the China Culture Collection of Microorganisms on April 24, 2024, with a deposit address of No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, and a deposit number of CGMCCNO.30427. The present invention isolated and obtained Stutzerimonas kunmingensis 9‑4 from a water sample of a crucian carp recirculating aquaculture system. The strain has a good removal effect on nitrate nitrogen, nitrite nitrogen and ammonia nitrogen in the water environment. At the same time, adding hematite under light conditions can significantly improve the removal rate of nitrate nitrogen, nitrite nitrogen and ammonia nitrogen by the Stutzerimonas kunmingensis, which can be applied to the removal of nitrate nitrogen, nitrite nitrogen and ammonia nitrogen in aquaculture water environment and aquaculture tail water.

The present invention belongs to the field of aquatic biological breeding, and specifically relates to a method and application of creating germplasm without intermuscular spines of economic fish for breeding. runx2b The economic fish, Megalobrama amblycephala, is gene-edited using a gene as the target gene. F0 generation fish with mutations are selected and cultured as parent fish. The F1 generation is produced by mating and breeding the F0 generation mutant male and female parents. F1 fish without intermuscular spines are screened and cultured to adult fish for use in cultivating a new germplasm of Megalobrama amblycephala without intermuscular spines. The present invention utilizes a gene mutation method to obtain a new germplasm of the economically cultivated fish, Megalobrama amblycephala, without intermuscular spines. This provides an improved method for solving the problem of the significant impact of intermuscular spines in the muscles of important aquacultured economic fish such as silver carp, bighead carp, carp, crucian carp, and bream in my country on their consumption and processing. It also provides a basis for subsequent research on the molecular formation mechanism of intermuscular spines, and has important scientific research value and industrial application value.

The invention provides a breeding method for constructing XX / XY sex determination all-female sterile fish and application. The method comprises the following steps: knocking out a catalytic enzyme coding gene of a fish sex steroid hormone synthesis pathway by using a gene editing technology to block the fish sex steroid hormone synthesis pathway, screening to obtain an effectively mutated homozygote XX sex genetic type pseudo male fish, and hybridizing the pseudo male fish with improved allotetraploid crucian and carp female fish to obtain the fish sex steroid hormone synthesis pathway. Therefore, an allotriploid population is obtained, and the population completely develops into female fishes and is sterile, so that the effect of obtaining all-female and sterile populations is achieved at the same time. The method provided by the invention has very strong applicability, and can be widely applied to aquaculture of fishes to obtain female unisexual groups of the fishes so as to improve the aquaculture yield, realize effective control on female fertility and protect ecological safety; the method has a wide application prospect in the fields of genetic breeding and ecological safety of aquaculture fishes.

The invention discloses enterobacter mori capable of resisting fish aeromonas and application of the enterobacter mori, the bacterial strain is classified and named as enterobacter mori SCH0241, the enterobacter mori SCH0241 is preserved in the China Center for Type Culture Collection on October 17, 2024, and the preservation number is CCTCC NO: M 20242236. The enterobacter mori SCH0241 disclosed by the invention is derived from the intestinal tract of high-stress-resistance healthy hybrid crucian carassius auratus No. 2, has good contact dependence antibacterial activity on aeromonas veronii, and can improve the capability of resisting aeromonas veronii infection of zebra fish. Through construction of an insertion mutation library and phenotype screening, it is found that a gene cluster of SCH0241, which exerts contact inhibition toxicity, is T6SS-2. The enterobacter mori SCH0241 disclosed by the invention provides a new powerful tool for biological prevention and treatment of fish diseases caused by aeromonas veronii.

The invention belongs to the field of fish breeding, and discloses a construction method and primers of a fast-growing red crucian carp homozygous strain, and the construction method comprises the following steps: preparing gRNA of a red crucian carp acvr2b gene, microinjecting a mixture of the gRNA and Cas9 protein into a one-cell stage fertilized egg of the red crucian carp, and screening to obtain an F0-generation gene knockout mutant; carrying out selfing on the F0-generation gene knockout mutant to obtain an F1-generation mutant, and screening to obtain a homozygous mutant; and selfing and propagating the homozygous mutant to obtain the product. The acvr2b homozygous knockout strain is successfully established in the red crucian carp through a CRISPR / Cas9 gene editing technology for the first time, the body weight of the obtained acvr2b mutant red crucian carp at the age of 12 months is increased by 30.68% compared with that of a wild type, the muscle protein content is increased by 13.81%, the intestinal digestion and absorption capacity is obviously improved, high-quality germplasm resources are provided for fish genetic breeding, and the acvr2b mutant red crucian carp has a good application prospect.

The invention belongs to the field of fish genetic breeding, and discloses a method for efficiently obtaining homozygous red crucian carp, which comprises the following steps: (1) carrying out artificial false fertilization on eggs of the red crucian carp to obtain fertilized eggs; (2) performing gene editing on the fertilized eggs; (3) incubating the fertilized eggs subjected to gene editing for 18-32 minutes, and then carrying out cold treatment; and (4) incubating the fertilized eggs after cold treatment to obtain the homozygous red crucian carp. According to the method disclosed by the invention, gene editing and gynogenesis methods are comprehensively utilized, the hatching rate, the survival rate and the homozygous rate are high while the high gene editing efficiency is maintained through the early cold shock treatment time and the proper cold shock treatment time, and the gynogenesis red crucian carp mutant with a high proportion can be obtained.

The invention belongs to the technical field of microorganisms, and particularly discloses an ethanol phase extract of rumex madaio roots and application of the ethanol phase extract. An ethanol phase extract RmEE is extracted from rumex madaio roots through a 55-95% ethanol solvent extraction method, and the ethanol phase extract RmEE is further separated and purified to obtain a purified component RmEE-F2. The ethanol phase extract RmEE and the purified component RmEE-F2 of the ethanol phase extract RmEE can inhibit staphylococcus aureus and vibrio cholerae, and can be used as bacteriostatic agents. The ethanol phase extract of the rumex madaio root can inhibit the pollution of staphylococcus aureus and vibrio cholerae in meat of crucian and penaeus vannamei which are stored at low temperature (4 DEG C), and can be applied to aquatic products; the method can also be used for preparing food or medicines, and the prepared food or medicines also have an antibacterial effect.

This invention discloses a molecular marker, primer pair, kit, and its application and method for identifying gynogenetic offspring of red crucian carp induced by blunt snout bream. The nucleotide sequence of the molecular marker is shown in SEQ ID NO.1. Based on resequencing analysis and genome alignment of gynogenetic offspring of red crucian carp induced by blunt snout bream, this invention screens for paternal gene fragments from blunt snout bream and develops primer pairs with blunt snout bream molecular markers and specificity. This effectively identifies gynogenetic offspring of red crucian carp induced by blunt snout bream, effectively avoiding the identification difficulties caused by morphological differences. The method of this invention is simple, rapid, and highly accurate, and can play an important role in the field of fish genetics and breeding.

The application belongs to the technical field of microorganisms, and specifically discloses a methanol phase extract of fruit of Phyllanthus emblica and application thereof. The methanol phase extract PeME is extracted from the fruit of Phyllanthus emblica by a methanol-chloroform extraction method, and a purified component PeME-F3 is obtained by further separation and purification of the methanol phase extract. The methanol phase extract PeME and the purified component PeME-F3 can inhibit Vibrio cholerae and Staphylococcus aureus, and can be used as bacteriostatic agents. The methanol phase extract of the fruit of Phyllanthus emblica can inhibit contamination of Vibrio cholerae and Staphylococcus aureus in crucian carp and Penaeus vannamei stored at low temperature, and can be applied to aquatic products; the methanol phase extract can also be used for preparation of food or medicine, and the prepared food or medicine also has the bacteriostatic effect.

The invention discloses a function and an application of a fish LIM and SH3 structural domain protein 1 (LASP1) gene in fish antiviral immune regulation, and particularly discloses a molecular mechanism and an application value of the LASP1 as an immune negative regulation factor in carassius auratus herpesvirus (CaHV) infection. Experiments prove that fish LASP1 has the function of remarkably inhibiting immune regulation generated by host interferon and can promote CaHV proliferation for the first time, and knock-down of the gene can effectively enhance host antiviral response and inhibit CaHV infection. Therefore, the lasp1 gene can be used as a new target for preventing and treating CaHV infection, is used for preparing a preparation for preventing or treating CaHV infection, or is used for gene editing of a cell model for preventing CaHV infection or a new crucian carp variety.

This invention discloses a method for co-culturing giant freshwater prawns (Macrobrachium rosenbergii) with shrimp, grass, and fish based on probiotic regulation, and its application. This invention constructs a three-dimensional ecological system with shrimp as the main body, grass as the protector, and fish as a supplement. Elodea and water spinach purify the water, silver carp and crucian carp decompose uneaten feed and feces, and a compound microbial preparation of Lactococcus gasseri C6a2 regulates water quality, while a biological bottom conditioner improves the bottom sediment, achieving precise ecological regulation of the aquaculture environment. This invention does not rely on chemical agents, effectively inhibits ammonia nitrogen accumulation, reduces disease incidence, and significantly improves the survival rate and size of giant freshwater prawns, achieving a win-win situation for both ecological and economic benefits.

The invention relates to the field of fishery experiments, and discloses a Chuzhou crucian carp hypoxia stress experiment device which comprises a breeding mechanism for breeding and an oxygen control mechanism for controlling oxygen, the breeding mechanism comprises a breeding box on the outermost side, a plurality of disturbance rods are connected to one side of the breeding box, a partition plate is fixedly connected to the interior of the breeding box, and a plurality of separation pipes are connected to the bottom of the partition plate; according to the device, water quality filtering monitoring can be continuously carried out, meanwhile, the aeration oxygen supply function is matched, it is ensured that the oxygen content of a water body is stable, meanwhile, various variables can be controlled to be kept stable, the stability of the water body is ensured, and the water quality is improved. The accuracy of final experimental data is ensured, and better data support is provided for subsequent breeding.

The present application belongs to the technical field of fish breeding, and discloses a method for efficiently inducing and rapidly identifying homologous tetraploid crucian male-nuclear development offspring, comprising the following steps: inducing parturition of female hybrid crucian and male homologous tetraploid crucian, obtaining hybrid crucian ovum, placing the ovum in ovary liquid, inactivating the ovum through ultraviolet lamp irradiation, stirring and uniformly mixing the ovum with sperm of the homologous tetraploid crucian to make the ovum "fertilized", without chromosome doubling treatment, and obtaining survival offspring after hatching and feeding, wherein a pair of oral papillae in the survival offspring is the male-nuclear development offspring. The hybrid crucian ovum selected by the present application contains a heterologous genome itself, has better compatibility, is beneficial to improving the hatching rate, can efficiently induce homologous tetraploid crucian male-nuclear development, and simultaneously utilizes the appearance characteristic of the male-nuclear development individual inheriting a pair of oral papillae from the father to rapidly identify the male-nuclear development offspring, so that the goal of rapidly identifying homologous tetraploid crucian male-nuclear development offspring is achieved, and the present application has important significance in fish genetic breeding.

The present application relates to the technical field of feeding of crucian carp, and discloses a compound functional feed for repairing carbonate alkali damage of crucian carp, which is composed of alpha-ketoglutaric acid, linoleic acid, laurylene and basic feed, wherein the weight ratio of the alpha-ketoglutaric acid accounts for 1.5% of the total weight of the compound functional feed for repairing carbonate alkali damage of crucian carp, and the weight ratio of the linoleic acid accounts for 0.7% of the total weight of the compound functional feed for repairing carbonate alkali damage of crucian carp. The compound functional feed for repairing carbonate alkali damage of crucian carp can significantly enhance the repair capacity of the tissue cells of the crucian carp, improve the disease resistance of the crucian carp, reduce the risk of diseases and promote the energy metabolism process of the crucian carp, so as to ensure the efficient use of energy and maintain the normal homeostasis of the body.

The present invention belongs to the technical field of fish genetic breeding, and specifically relates to a method for creating a fertile alloheptaploid silver crucian carp gynogenetic line that is resistant to herpes simplex virus. First, a gynogenetic triploid silver crucian carp mother is bred with a sexually reproducing diploid amblycephala bream father to obtain offspring G1, and then individuals whose genomes have been integrated with a set of amblycephala bream sperm chromosome sets are screened from offspring G1, i.e., alloheptaploid silver crucian carp, which has fertile gynogenetic ability. Herpes simplex virus infection experiments have confirmed that the alloheptaploid silver crucian carp clone line has 100% resistance to herpes simplex virus. The breeding route provided by the present application can create a new silver crucian carp strain with significant resistance to herpes simplex virus and maintaining gynogenetic ability, which is an important way to cultivate new silver crucian carp varieties and has broad application prospects.

The invention relates to an ecological mandarin fish breeding method based on natural breeding of bait fishes, and belongs to the technical field of aquaculture. According to the method, a crayfish culture pond or a river crab culture pond serves as an ecological mandarin fish culture pond, and aquatic plants with the coverage rate larger than 30% are planted. Propagation and growth characteristics of indigenous crucian carps are ingeniously utilized, the indigenous crucian carps are put from November of the previous year to January of the next year, and feed residues and excrement for breeding crayfishes or river crabs are used for providing food for the crucian carps; the aquatic plants provide inhabiting and molting places for the crayfishes or the river crabs, provide spawning places for the crucian carps, and purify water to form a stable ecological system. Large-size mandarin fish fries are put in the middle and last ten days of May in the next year, natural breeding of crucian carps provides continuous and palatable live bait fishes for mandarin fish, an efficient ecological circulation system is formed, and rapid growth of mandarin fish is promoted. The usage amount of the water quality improver and fishery medicine is zero, and mandarin fish, crayfish or river crab and crucian are harvested in autumn at the same time.

The application belongs to the field of fish breeding, and discloses a construction method and primers of a homozygous line of fast-growing red crucian carp. acvr2b The construction method comprises the following steps: preparing gRNA of a red crucian carp gene, microinjecting a mixture of the gRNA and a Cas9 protein into a one-cell fertilized egg of the red crucian carp, screening to obtain F0 generation gene knockout mutants, self-crossing the F0 generation gene knockout mutants to obtain F1 generation mutants, screening to obtain homozygous mutants, and self-crossing the homozygous mutants to propagate. acvr2b The application successfully establishes a homozygous knockout line in the red crucian carp for the first time through a CRISPR / Cas9 gene editing technology. acvr2b The mutant red crucian carp obtained in the application has a body weight increased by 30.68% and a muscle protein content increased by 13.81% compared with a wild type at 12 months of age, and has a significantly improved intestinal digestion and absorption capacity, thereby providing high-quality germplasm resources for fish genetic breeding and having a good application prospect.

The invention relates to the technical field of fish cultivation, in particular to a method for cultivating all-male tetraploid crucian carp, which comprises the following steps of: performing artificial spawning induction by taking gynogenesis homologous diploid crucian carp as a female parent and taking androgenesis homologous diploid crucian carp as a male parent; wherein the sex chromosome type of the gynogenesis homologous diploid crucian carp is XX and generates non-reduced diploid ovum, and the sex chromosome type of a male individual in the androgenesis homologous diploid crucian carp is XY or YY and generates non-reduced diploid sperm; artificial insemination is carried out, obtained fertilized eggs are hatched and cultured to sexual maturity, and survival offspring is the all-male tetraploid crucian carp population. Based on the characteristic that gynogenesis homologous diploid carassius auratus and androgenesis homologous diploid carassius auratus both generate non-reducing gametes, the gynogenesis homologous diploid carassius auratus and the androgenesis homologous diploid carassius auratus are subjected to artificial insemination, and all-male tetraploid The all-male tetraploid crucian carp can reach a sexual maturity state after being only 120 days old, diploid sperms can be stably generated, and the reproductive performance is improved.

The invention belongs to the technical field of fish genetic breeding, and particularly relates to a creation method of a crucian carp herpesvirus resistant fertile heterologous heptaploid crucian carp gynogenetic germline. The method comprises the following steps: firstly, breeding a gynogenesis double triploid prussian carp female parent and a sexual reproduction diploid megalobrama amblycephala male parent to obtain a filial generation G1, and then screening an individual of which the genome is fused with a set of megalobrama amblycephala sperm chromosome group from the filial generation G1, namely the heterologous heptaploid prussian carp which has the gynogenesis capacity. Cassius auratus gibelio herpesvirus infection experiments prove that the heterologous heptaploid Cassius auratus gibelio clone line has 100% anti-herpesvirus ability, and the breeding route provided by the invention can create a new Cassius auratus gibelio line with significant anti-herpesvirus ability and maintenance of gynogenesis ability, is an important way for breeding new Cassius auratus gibelio varieties, and has wide application prospects.

The invention relates to the technical field of fish genetic breeding, and discloses a creation method, an identification method and application of alkali-resistant heterologous heptaploid crucian carp. The characteristic that sperms and eggs of new double-triploid prussian carp are combined to form fertilized eggs is utilized, sperms of male leuciscus waleckii are integrated into eggs of female new double-triploid prussian carp in a non-subtrahend fusion mode, and a heteroheptaploid prussian carp group is created. Specific sequences of the heterogenous heptaploid carassius auratus gibelio are screened and explored, molecular marker primers are designed, breeding screening in the future is facilitated, further carbonate alkalinity stress experiments are carried out, and it is found that compared with new double-triploid carassius auratus gibelio, the alkali resistance of the heterogenous heptaploid carassius auratus gibelio is remarkably improved, and the heterogenous heptaploid carassius auratus gibelio is expected to be cultured in saline-alkali water areas.

This application discloses a method for periodically feeding vine tea ultrafine powder in aquaculture and its application. The method includes the following steps: mixing vine tea ultrafine powder into aquatic animal feed at an addition rate of 1% to 8% of the total feed weight, and feeding it using a periodic feeding pattern; the periodic feeding pattern consists of continuous feeding for 7 days, followed by a 7-21 day break, which constitutes one cycle. This application, by pulverizing vine tea to an optimal particle size of 300 mesh, and by optimizing the optimal addition rate of 4% and the periodic feeding strategy of "7 days of continuous use, 14 days of break," fully utilizes the antioxidant and immune-enhancing effects of vine tea while effectively avoiding the potential liver and kidney burden and immunosuppression risks associated with long-term continuous use. Large-scale application trials show that this method achieves a protection rate of 52.91% to 65.14% in various aquatic animals, including crucian carp, largemouth bass, whiteleg shrimp, carp, and tilapia, demonstrating significant economic benefits and promising prospects for industrial application.

The invention discloses a method for inducing gynogenesis of Sinilabeo notabilis by white crucian carp sperms and application thereof, and the method for inducing gynogenesis of Sinilabeo notabilis by white crucian carp sperms comprises the following steps: (1) collecting and diluting white crucian carp sperms, and then carrying out inactivation treatment by ultraviolet lamp irradiation; (2) mixing the inactivated seminal fluid of the carassius auratus and the inactivated mature eggs of the sinilabeo nolatus, performing dry insemination, then pouring the mixture into cold water for treatment, putting fertilized eggs into a normal-temperature water incubation tank for incubation after cold treatment is finished, and keeping running water to enable the fertilized eggs to float along with water flow and not to sink to the bottom; and (3) after fry are hatched from the fertilized eggs, culturing to obtain gynogenetic sinilabeo nolabeo. According to the method, the carassius auratus sperms are adopted to induce the gynogenesis of the semilabeo nolabeo to successfully breed and obtain the gynogenesis semilabeo nolabeo offspring, which is the first time in the artificial induction gynogenesis and germplasm improvement of the semilabeo nolabeo. In the breeding process, it is found that the 6-month-old semilabeo notabilis has the advantages that the growth speed is high, and the low-temperature resistance is remarkably higher than that of the parent semilabeo notabilis.

The utility model relates to the technical field of crucian breeding, and discloses a temperature-control oxygen-increasing hatching device for artificial breeding of Chuzhou crucian carps, which comprises a hatching box, a hatching cavity, a circulating cavity and an equipment cavity, the hatching cavity, the circulating cavity and the equipment cavity are arranged in the hatching box, a first seedling raising layer and a second seedling raising layer are sequentially fixed in the hatching cavity from top to bottom, and a rotating hole is arranged on the inner wall between the hatching cavity and the circulating cavity. A stirring column is rotatably arranged in the rotating hole in a penetrating mode, a plurality of stirring fan blades are fixedly connected to the outer wall of the stirring column located in the hatching cavity in a sleeving mode, a plurality of water conveying holes are formed in the inner wall between the hatching cavity and the circulating cavity, and a plurality of blocking columns are slidably arranged in the water conveying holes in a penetrating mode. The crucian egg hatching device is reasonable in structure, convenient to use and high in working performance, can help to hatch crucian eggs, can purify water in the hatching device, can promote dispersion of the crucian eggs and fry, can regulate and control the temperature of culture water, further effectively improves the hatching rate of the crucian eggs, and can regulate the circulating speed of the water in the device.

The present application relates to the technical field of fish farming, and especially relates to a fish nest for collecting fish eggs of Pengze crucian carp spawning. The present application provides a fish nest for collecting fish eggs of Pengze crucian carp spawning, which can centrally hang waterweeds, facilitate the taking of the waterweeds and the collection of the fish eggs. The fish nest for collecting fish eggs of Pengze crucian carp spawning comprises a floating frame, fixing blocks, air bags, a collecting mechanism and a lifting mechanism. The floating frame is connected with four fixing blocks at the top. The fixing blocks are connected with air bags. The floating frame is provided with the collecting mechanism. The floating frame is provided with the lifting mechanism. The present application suspends the waterweeds by the collecting net, thereby simulating the suitable spawning environment of the Pengze crucian carp and facilitating the quick collection of the fish eggs by the breeders.

The present invention provides a kind of breeding method that improves the disease resistance of Pengze crucian carp myxosporeans, comprising the following steps: S1. breeding environment treatment, S2. purchasing healthy fry, S3. fry disinfection, S4. fry delivery, S5. feeding management, S6. water quality management, S7. disease prevention and detection, S8. emergency treatment. In the fry disinfection link, Pengze crucian carp fry are soaked by using a biological disinfectant composed of tea saponin, photosensitizing component, carvacrol, artesunate, and choline-lactic acid ionic liquid, and supplemented with LED light irradiation. These disinfecting components can work together after penetrating into the inside of the myxosporeans, inhibiting their growth and reproduction, thereby achieving the effect of effectively killing the myxosporeans. In the emergency treatment link, the insecticide composed of polysaccharide-mercaptosuccinic acid conjugate, antimicrobial peptide, artesunate, nano zinc oxide, triptolide, and carvacrol is used, and through the synergistic effect between the components, multi-target efficient killing of myxosporeans is achieved.

The invention belongs to the technical field of molecular breeding of aquatic products, and particularly discloses a molecular marker combination for identifying new species of carassius auratus and carassius auratus No.2 and application of the molecular marker combination. Based on a whole genome re-sequencing technology, eight SNPs and three SV sites are obtained through screening, primers are designed according to site information and used for judging carassius auratus and carassius auratus No.2, and a combined judgment rule is that if fragments, amplified by 11 primers, of a to-be-detected sample are double bands (heterozygous type), the carassius auratus is judged as the carassius auratus, and if the fragments, amplified by 11 primers, of the to-be-detected sample are double bands (heterozygous type), of the to-be-detected sample are double bands (heterozygous type); if double bands (heterozygous type) and the carassius auratus type (male parent homozygous type) exist at the same time and the ratio of the double bands to the carassius auratus type (male parent homozygous type) is 0.375-2.667, the carassius auratus is judged to be carassius auratus No. 2. According to the invention, a double verification mechanism of the SNP marker and the SV fragment is innovatively combined, a molecular marker combination suitable for identifying a new aquatic product crucian carp series fish is established, and the detection accuracy is relatively high. The molecular marker provided by the invention can be applied to carassius auratus variety identification, filial generation screening and molecular assisted breeding, and has important application value.

The invention relates to the field of fish school feeding detection, and discloses a sampling device for Chuzhou crucian carp virus detection, which comprises a protection frame, a belt conveyor connected to the protection frame, a detection mechanism arranged above the belt conveyor, first electric slide rails fixedly connected to two sides of the protection frame, and a storage box in transmission connection with the output ends of the first electric slide rails. A binding belt is connected between the two symmetrical storage boxes, a third electric sliding rail is connected between the two parallel first electric sliding rails, the moving end of the third electric sliding rail is in transmission connection with a second electric sliding rail, a first power box is in transmission connection between the two second electric sliding rails, and the executing end of the first power box is in transmission connection with a second power box. The crucian and the sampling pen can be quickly limited to reach a specified position and a specified angle, sampling at different sampling points can be carried out, repeated continuous sampling can be conveniently carried out only by replacing the corresponding sampling pen, the number of samples is increased, and the overall detection effect is improved.

The present application relates to fish breeding technology field, especially to a kind of all-male tetraploid crucian carp breeding method, comprising the following steps: with gynogenesis homologous diploid crucian carp as female parent, and androgenesis homologous diploid crucian carp as male parent, artificial delivery is carried out;Wherein, the gynogenesis homologous diploid crucian carp sex chromosome type is XX, produces non-reduction diploid egg, and the androgenesis homologous diploid crucian carp male individual sex chromosome type is XY or YY, produces non-reduction diploid sperm;Artificial insemination is carried out, and the obtained zygote is hatched, and cultured to sexual maturity, and the surviving offspring is all-male tetraploid crucian carp population.The present application is based on the characteristics that gynogenesis homologous diploid crucian carp and androgenesis homologous diploid crucian carp both produce non-reduction gametes, artificial insemination is carried out, and all-male tetraploid crucian carp can be prepared on a large scale.The all-male tetraploid crucian carp only needs 120 days of age to reach sexual maturity, can stably produce diploid sperm, and the reproductive performance is improved.

The invention discloses an application of a fructus psoraleae extract in carassius auratus gibelio hypoxia stress, and relates to the technical field of application of fructus psoraleae, and the application comprises the step of adding the fructus psoraleae extract into a feed so as to improve the hypoxia tolerance or oxidation resistance of carassius auratus gibelio. According to the invention, the fructus psoraleae extract is added into the feed, so that the purpose of improving the hypoxia tolerance or oxidation resistance of the carassius auratus gibelio is achieved.

The present invention relates to the field of fish farming technology, and in particular to a method for inhibiting gonadal development in Pengze crucian carp. The method comprises the following steps: preparing a circular canvas pool, cement pool, or culture barrel with a diameter of 6-10 meters and a height of 1-1.5 meters, equipped with a microporous oxygenation device; cultivating bioflocs in the circular canvas pool, cement pool, or culture barrel to culture Pengze crucian carp; and inhibiting gonadal development in Pengze crucian carp by managing biofloc suspended solids (TSS) concentration, alkalinity, carbon-nitrogen ratio, water quality, and feeding. The present invention, developed based on biofloc technology, can inhibit gonadal development in Pengze crucian carp, addressing the problem of premature gonadal maturation in Pengze crucian carp farming without affecting growth and muscle nutrients, and increasing meat yield. The present invention provides a new aquaculture method for the Pengze crucian carp farming industry that can address premature gonadal maturation, thereby achieving the goal of improving the quality and efficiency of Pengze crucian carp farming.