58 results about "Crucian carp" patented technology

The invention discloses enterobacter mori capable of resisting fish aeromonas and application of the enterobacter mori, the bacterial strain is classified and named as enterobacter mori SCH0241, the enterobacter mori SCH0241 is preserved in the China Center for Type Culture Collection on October 17, 2024, and the preservation number is CCTCC NO: M 20242236. The enterobacter mori SCH0241 disclosed by the invention is derived from the intestinal tract of high-stress-resistance healthy hybrid crucian carassius auratus No. 2, has good contact dependence antibacterial activity on aeromonas veronii, and can improve the capability of resisting aeromonas veronii infection of zebra fish. Through construction of an insertion mutation library and phenotype screening, it is found that a gene cluster of SCH0241, which exerts contact inhibition toxicity, is T6SS-2. The enterobacter mori SCH0241 disclosed by the invention provides a new powerful tool for biological prevention and treatment of fish diseases caused by aeromonas veronii.

This invention discloses a molecular marker, primer pair, kit, and its application and method for identifying gynogenetic offspring of red crucian carp induced by blunt snout bream. The nucleotide sequence of the molecular marker is shown in SEQ ID NO.1. Based on resequencing analysis and genome alignment of gynogenetic offspring of red crucian carp induced by blunt snout bream, this invention screens for paternal gene fragments from blunt snout bream and develops primer pairs with blunt snout bream molecular markers and specificity. This effectively identifies gynogenetic offspring of red crucian carp induced by blunt snout bream, effectively avoiding the identification difficulties caused by morphological differences. The method of this invention is simple, rapid, and highly accurate, and can play an important role in the field of fish genetics and breeding.

The application belongs to the technical field of microorganisms, and specifically discloses a methanol phase extract of fruit of Phyllanthus emblica and application thereof. The methanol phase extract PeME is extracted from the fruit of Phyllanthus emblica by a methanol-chloroform extraction method, and a purified component PeME-F3 is obtained by further separation and purification of the methanol phase extract. The methanol phase extract PeME and the purified component PeME-F3 can inhibit Vibrio cholerae and Staphylococcus aureus, and can be used as bacteriostatic agents. The methanol phase extract of the fruit of Phyllanthus emblica can inhibit contamination of Vibrio cholerae and Staphylococcus aureus in crucian carp and Penaeus vannamei stored at low temperature, and can be applied to aquatic products; the methanol phase extract can also be used for preparation of food or medicine, and the prepared food or medicine also has the bacteriostatic effect.

This invention discloses a method for co-culturing giant freshwater prawns (Macrobrachium rosenbergii) with shrimp, grass, and fish based on probiotic regulation, and its application. This invention constructs a three-dimensional ecological system with shrimp as the main body, grass as the protector, and fish as a supplement. Elodea and water spinach purify the water, silver carp and crucian carp decompose uneaten feed and feces, and a compound microbial preparation of Lactococcus gasseri C6a2 regulates water quality, while a biological bottom conditioner improves the bottom sediment, achieving precise ecological regulation of the aquaculture environment. This invention does not rely on chemical agents, effectively inhibits ammonia nitrogen accumulation, reduces disease incidence, and significantly improves the survival rate and size of giant freshwater prawns, achieving a win-win situation for both ecological and economic benefits.

The present application belongs to the technical field of fish breeding, and discloses a method for efficiently inducing and rapidly identifying homologous tetraploid crucian male-nuclear development offspring, comprising the following steps: inducing parturition of female hybrid crucian and male homologous tetraploid crucian, obtaining hybrid crucian ovum, placing the ovum in ovary liquid, inactivating the ovum through ultraviolet lamp irradiation, stirring and uniformly mixing the ovum with sperm of the homologous tetraploid crucian to make the ovum "fertilized", without chromosome doubling treatment, and obtaining survival offspring after hatching and feeding, wherein a pair of oral papillae in the survival offspring is the male-nuclear development offspring. The hybrid crucian ovum selected by the present application contains a heterologous genome itself, has better compatibility, is beneficial to improving the hatching rate, can efficiently induce homologous tetraploid crucian male-nuclear development, and simultaneously utilizes the appearance characteristic of the male-nuclear development individual inheriting a pair of oral papillae from the father to rapidly identify the male-nuclear development offspring, so that the goal of rapidly identifying homologous tetraploid crucian male-nuclear development offspring is achieved, and the present application has important significance in fish genetic breeding.

The present application relates to the technical field of feeding of crucian carp, and discloses a compound functional feed for repairing carbonate alkali damage of crucian carp, which is composed of alpha-ketoglutaric acid, linoleic acid, laurylene and basic feed, wherein the weight ratio of the alpha-ketoglutaric acid accounts for 1.5% of the total weight of the compound functional feed for repairing carbonate alkali damage of crucian carp, and the weight ratio of the linoleic acid accounts for 0.7% of the total weight of the compound functional feed for repairing carbonate alkali damage of crucian carp. The compound functional feed for repairing carbonate alkali damage of crucian carp can significantly enhance the repair capacity of the tissue cells of the crucian carp, improve the disease resistance of the crucian carp, reduce the risk of diseases and promote the energy metabolism process of the crucian carp, so as to ensure the efficient use of energy and maintain the normal homeostasis of the body.

The invention relates to an ecological mandarin fish breeding method based on natural breeding of bait fishes, and belongs to the technical field of aquaculture. According to the method, a crayfish culture pond or a river crab culture pond serves as an ecological mandarin fish culture pond, and aquatic plants with the coverage rate larger than 30% are planted. Propagation and growth characteristics of indigenous crucian carps are ingeniously utilized, the indigenous crucian carps are put from November of the previous year to January of the next year, and feed residues and excrement for breeding crayfishes or river crabs are used for providing food for the crucian carps; the aquatic plants provide inhabiting and molting places for the crayfishes or the river crabs, provide spawning places for the crucian carps, and purify water to form a stable ecological system. Large-size mandarin fish fries are put in the middle and last ten days of May in the next year, natural breeding of crucian carps provides continuous and palatable live bait fishes for mandarin fish, an efficient ecological circulation system is formed, and rapid growth of mandarin fish is promoted. The usage amount of the water quality improver and fishery medicine is zero, and mandarin fish, crayfish or river crab and crucian are harvested in autumn at the same time.

The application belongs to the field of fish breeding, and discloses a construction method and primers of a homozygous line of fast-growing red crucian carp. acvr2b The construction method comprises the following steps: preparing gRNA of a red crucian carp gene, microinjecting a mixture of the gRNA and a Cas9 protein into a one-cell fertilized egg of the red crucian carp, screening to obtain F0 generation gene knockout mutants, self-crossing the F0 generation gene knockout mutants to obtain F1 generation mutants, screening to obtain homozygous mutants, and self-crossing the homozygous mutants to propagate. acvr2b The application successfully establishes a homozygous knockout line in the red crucian carp for the first time through a CRISPR / Cas9 gene editing technology. acvr2b The mutant red crucian carp obtained in the application has a body weight increased by 30.68% and a muscle protein content increased by 13.81% compared with a wild type at 12 months of age, and has a significantly improved intestinal digestion and absorption capacity, thereby providing high-quality germplasm resources for fish genetic breeding and having a good application prospect.

This application discloses a method for periodically feeding vine tea ultrafine powder in aquaculture and its application. The method includes the following steps: mixing vine tea ultrafine powder into aquatic animal feed at an addition rate of 1% to 8% of the total feed weight, and feeding it using a periodic feeding pattern; the periodic feeding pattern consists of continuous feeding for 7 days, followed by a 7-21 day break, which constitutes one cycle. This application, by pulverizing vine tea to an optimal particle size of 300 mesh, and by optimizing the optimal addition rate of 4% and the periodic feeding strategy of "7 days of continuous use, 14 days of break," fully utilizes the antioxidant and immune-enhancing effects of vine tea while effectively avoiding the potential liver and kidney burden and immunosuppression risks associated with long-term continuous use. Large-scale application trials show that this method achieves a protection rate of 52.91% to 65.14% in various aquatic animals, including crucian carp, largemouth bass, whiteleg shrimp, carp, and tilapia, demonstrating significant economic benefits and promising prospects for industrial application.

The invention discloses a method for inducing gynogenesis of Sinilabeo notabilis by white crucian carp sperms and application thereof, and the method for inducing gynogenesis of Sinilabeo notabilis by white crucian carp sperms comprises the following steps: (1) collecting and diluting white crucian carp sperms, and then carrying out inactivation treatment by ultraviolet lamp irradiation; (2) mixing the inactivated seminal fluid of the carassius auratus and the inactivated mature eggs of the sinilabeo nolatus, performing dry insemination, then pouring the mixture into cold water for treatment, putting fertilized eggs into a normal-temperature water incubation tank for incubation after cold treatment is finished, and keeping running water to enable the fertilized eggs to float along with water flow and not to sink to the bottom; and (3) after fry are hatched from the fertilized eggs, culturing to obtain gynogenetic sinilabeo nolabeo. According to the method, the carassius auratus sperms are adopted to induce the gynogenesis of the semilabeo nolabeo to successfully breed and obtain the gynogenesis semilabeo nolabeo offspring, which is the first time in the artificial induction gynogenesis and germplasm improvement of the semilabeo nolabeo. In the breeding process, it is found that the 6-month-old semilabeo notabilis has the advantages that the growth speed is high, and the low-temperature resistance is remarkably higher than that of the parent semilabeo notabilis.

The utility model relates to the technical field of crucian breeding, and discloses a temperature-control oxygen-increasing hatching device for artificial breeding of Chuzhou crucian carps, which comprises a hatching box, a hatching cavity, a circulating cavity and an equipment cavity, the hatching cavity, the circulating cavity and the equipment cavity are arranged in the hatching box, a first seedling raising layer and a second seedling raising layer are sequentially fixed in the hatching cavity from top to bottom, and a rotating hole is arranged on the inner wall between the hatching cavity and the circulating cavity. A stirring column is rotatably arranged in the rotating hole in a penetrating mode, a plurality of stirring fan blades are fixedly connected to the outer wall of the stirring column located in the hatching cavity in a sleeving mode, a plurality of water conveying holes are formed in the inner wall between the hatching cavity and the circulating cavity, and a plurality of blocking columns are slidably arranged in the water conveying holes in a penetrating mode. The crucian egg hatching device is reasonable in structure, convenient to use and high in working performance, can help to hatch crucian eggs, can purify water in the hatching device, can promote dispersion of the crucian eggs and fry, can regulate and control the temperature of culture water, further effectively improves the hatching rate of the crucian eggs, and can regulate the circulating speed of the water in the device.

The present application relates to the technical field of fish farming, and especially relates to a fish nest for collecting fish eggs of Pengze crucian carp spawning. The present application provides a fish nest for collecting fish eggs of Pengze crucian carp spawning, which can centrally hang waterweeds, facilitate the taking of the waterweeds and the collection of the fish eggs. The fish nest for collecting fish eggs of Pengze crucian carp spawning comprises a floating frame, fixing blocks, air bags, a collecting mechanism and a lifting mechanism. The floating frame is connected with four fixing blocks at the top. The fixing blocks are connected with air bags. The floating frame is provided with the collecting mechanism. The floating frame is provided with the lifting mechanism. The present application suspends the waterweeds by the collecting net, thereby simulating the suitable spawning environment of the Pengze crucian carp and facilitating the quick collection of the fish eggs by the breeders.

The present application relates to fish breeding technology field, especially to a kind of all-male tetraploid crucian carp breeding method, comprising the following steps: with gynogenesis homologous diploid crucian carp as female parent, and androgenesis homologous diploid crucian carp as male parent, artificial delivery is carried out;Wherein, the gynogenesis homologous diploid crucian carp sex chromosome type is XX, produces non-reduction diploid egg, and the androgenesis homologous diploid crucian carp male individual sex chromosome type is XY or YY, produces non-reduction diploid sperm;Artificial insemination is carried out, and the obtained zygote is hatched, and cultured to sexual maturity, and the surviving offspring is all-male tetraploid crucian carp population.The present application is based on the characteristics that gynogenesis homologous diploid crucian carp and androgenesis homologous diploid crucian carp both produce non-reduction gametes, artificial insemination is carried out, and all-male tetraploid crucian carp can be prepared on a large scale.The all-male tetraploid crucian carp only needs 120 days of age to reach sexual maturity, can stably produce diploid sperm, and the reproductive performance is improved.

The invention discloses an application of a fructus psoraleae extract in carassius auratus gibelio hypoxia stress, and relates to the technical field of application of fructus psoraleae, and the application comprises the step of adding the fructus psoraleae extract into a feed so as to improve the hypoxia tolerance or oxidation resistance of carassius auratus gibelio. According to the invention, the fructus psoraleae extract is added into the feed, so that the purpose of improving the hypoxia tolerance or oxidation resistance of the carassius auratus gibelio is achieved.

The invention is suitable for the technical field of probiotic postbiotics, and provides an application of lactobacillus casei postbiotics in improving the immune function of crucian carps. In order to solve the problem of pollution of antibiotics to the culture water environment, the antibiotics are replaced by the postbiotics obtained by inactivating the lactobacillus casei, so that the problems of drug resistance, drug residues, edible safety and the like are solved, the growth performance of aquatic animals is improved, and the yield of the aquatic animals is increased on the basis of realizing green culture. By means of the lactobacillus casei, the immune function of the crucian is enhanced, and the immune response of the crucian is improved.

The invention belongs to the field of fish genetic breeding, and particularly relates to a method for preparing sterile triploid fish with a tyr gene knocked out, which comprises the following steps: S1, knocking out wild type female diploid red crucian carp and male autotetraploid crucian carp high mutants through the tyr gene, and screening out the female diploid red crucian carp high mutant and the male autotetraploid crucian carp high mutant; and S2, hybridizing the female diploid red crucian carp high mutant and the male autotetraploid crucian carp high mutant to prepare the tyr gene knockout sterile triploid crucian carp homozygous mutant on a large scale. By utilizing the method, the triploid fishes with the accurately improved characters are prepared on a large scale, and the problem of biological safety is solved due to the natural sterility characteristics of the triploid fishes, so that the method has important practical value and ecological significance for genetic breeding and sustainable development in aquaculture.

A method for tracing the origin of crucian carp based on muscle tissue fatty acid spectrum relates to the field of crucian carp origin tracing. The method solves the problem of the ecological destruction of the Yangtze River Basin, the continuous reduction of aquatic product resources and the sharp decline of the number of crucian carp due to the influence of water conservancy construction, overfishing and other factors. The method comprises the following steps: step 1, pretreating crucian carp muscle tissue to obtain fish oil; step 2, performing methyl esterification treatment on the fish oil obtained in step 1; step 3, detecting the characteristic fatty acid content of the crucian carp muscle tissue by using a GC-MS method; step 4, constructing a Yangtze River and non-Yangtze River crucian carp origin discrimination analysis model and a Yangtze River different reach crucian carp origin discrimination analysis model and performing origin discrimination to realize the origin prediction of the Yangtze River different reach crucian carp. The method is also suitable for the field of geographical tracing application of fatty acid detection.

The present application belongs to the field of fish breeding, and discloses a method for simultaneously breeding natural gynogenesis of Procyprinus and hybrid offspring of Cyprinus carpio, comprising the following steps: taking Procyprinus as the female parent and taking red crucian carp as the male parent to perform special pond breeding, after artificial induction of parturition, performing artificial insemination after the parent fish is in estrus, then performing flow water hatching on the fertilized eggs, after the fry is hatched, performing feeding, and then performing detection and screening on the fed fish, thereby obtaining the natural gynogenesis of Procyprinus and the hybrid offspring of Cyprinus carpio. The present application changes the activity of the ovum and sperm of the parent fish by performing distant hybridization of Procyprinus and red crucian carp and combining with specific types and dosages of parturition inducers, and thereby obtains the natural gynogenesis of Procyprinus and the hybrid offspring of Cyprinus carpio which are both sexually fertile.

The invention discloses a compound beverage and a preparation method thereof, and belongs to the technical field of beverage preparation. The compound beverage is prepared by the following steps: step 1, mixing and fermenting angelica sinensis, codonopsis pilosula, lotus seeds, phaseolus calcaratus, poria cocos, day lily, coix seeds, Chinese wolfberry fruits, Chinese dates and stevia rebaudiana; step 2, carrying out mixed fermentation on the Taihe black chicken meat, the pig trotters, the crucian meat, the donkey-hide gelatin powder, the compound enzyme and the enzyme activator; and step 3, mixing the materials obtained in the step 1 and the step 2 to obtain the compound beverage. Compared with the prior art, the prepared compound beverage has the advantages of being rich in nutritional ingredients, good in stability, high in protein and amino acid content, resistant to oxidation, free of peculiar smell after being added with meat ingredients and the like.

The present application belongs to the field of fish sex determination system identification, and discloses a molecular marker primer pair for identifying the sex determination system of homologous tetraploid crucian carp, a kit and application. The molecular marker primer pair can not be limited by the growth stage of the homologous tetraploid crucian carp, saves time cost, overcomes the limitation of low evolution degree of sex chromosomes of the homologous tetraploid crucian carp, and also provides a new technical means for determining the sex determination system of fish. The molecular marker primer pair, the kit and the application method are low in cost, simple in operation, rapid, accurate, and can be applied to identifying the sex determination system of homologous tetraploid crucian carp, providing a more convenient method for determining the sex determination system of homologous tetraploid crucian carp. Meanwhile, the molecular marker primer pair can also be used for identifying the sex of the androgenetic offspring of the homologous tetraploid crucian carp.

The invention relates to the technical field of fish phagostimulants, and discloses an emulsified milk-fishy phagostimulant and a preparation method thereof. Comprising the following raw materials: fresh chicken liver, fructose, sodium bicarbonate, table salt, chicken oil, ethyl maltol, vanillin, capric acid, dodecanoic acid, myristic acid, gamma-nonanolactone, delta-decalactone, gamma-octyl lactone, delta-dodecalactone, milk lactone, 3-hydroxy-2-butanone, an emulsifier and a cosolvent. Chicken livers and milk-flavor components are gradually added through a Maillard reaction, and the crucian carp phagostimulant with natural viscera fragrance and milk flavor is prepared. Compared with a control group, the feed attractant has the advantages that the feed attractant rate is increased by 79%, the feed attractant rate is increased by 15% compared with a single chicken liver Maillard reaction group, the feed coefficient is low, and the advanced level of white crucian carp culture is achieved. Great popularization and utilization values are realized.

The invention discloses a crucian egg dispersing, fixing and micro-injection device, and relates to the technical field of fish egg micro-injection, the crucian egg dispersing, fixing and micro-injection device comprises a base and a micro-injection instrument, the upper end of the base is provided with a box body, the box body is internally provided with a partition plate, the partition plate divides the interior of the box body into a collecting cavity and a water collecting cavity, a top plate is arranged above the box body, and the top plate is provided with a water outlet; a top plate is arranged in the box body, a first electric telescopic rod is arranged at the upper end of the top plate, the telescopic end of the first electric telescopic rod penetrates through the top plate and is connected with a microinjection instrument, the rotary table is arranged in the box body, four device grooves are formed in the side wall of the rotary table, and the four device grooves are formed in the circumferential direction of the rotary table at equal intervals; and the four roe dispersing assemblies are arranged on the inner sides of the four device grooves correspondingly. The device can continuously and automatically disperse and fix crucian eggs, perform micro-injection and discharge and collect the crucian eggs, so that the working efficiency of micro-injection of the crucian eggs is greatly improved.

The present application discloses a strain of Enterobacter mori against Aeromonas veronii and its application, which is classified and named as Enterobacter mori (SCH0241). Enterobacter mori SCH0241, preserved in China Center for Type Culture Collection on October 17, 2024, with a preservation number of CCTCC NO: M 20242236. The Enterobacter mori SCH0241 of the present application is derived from the intestine of a highly stress-resistant healthy hybrid crucian carp “Hefang crucian carp No. 2”, has good contact-dependent bacteriostatic activity against Aeromonas veronii, and can improve the ability of zebrafish to resist Aeromonas veronii infection. Through the construction of an insertion mutation library and phenotype screening, it is found that the gene cluster of T6SS-2 of SCH0241 plays a contact inhibition toxicity. The Enterobacter mori SCH0241 of the present application provides a new powerful tool for the biological control of fish diseases caused by Aeromonas veronii.

The invention discloses a whole-process primary processing system for crucian carps, and relates to the field of aquatic product processing equipment.The whole-process primary processing system comprises a stunning device, a scale scraping device, a laparotomy and viscera removing device, a gill removing and cleaning device and a storage device which are arranged in sequence, the crucian carps are treated by the stunning device to enter a syncope state and then conveyed to the scale scraping device to complete scale removal, and the fish scales are removed; the scaled fish body enters a belly-cutting and viscera-removing device, is stably clamped by oppositely arranged vertical conveyor belts and is conveyed forwards, and meanwhile, a belly-cutting knife and a digging spoon at the lower part cooperatively act to sequentially complete belly cutting and viscera digging; then, the fish body enters a gill removing and cleaning device, after the fish body is fixed by a clamping mechanism, gill removing cutters on the two sides are driven by a driving mechanism to accurately cut off fish gill, and the fish gill is finally washed by a cleaning spray head in the process of transferring the fish body to a storage device; finally, the treated fish bodies fall into a storage box to be collected. The full-process automatic processing of crucian carps from stunning to storage is realized, all procedures are smoothly linked, and the processing efficiency is high.

The application discloses a kind of based on photoperiod regulation's carp yield increasing breeding method and system, by obtaining image data in carp breeding site, determine the growth stage of carp, and according to it fish population selection and grouping are carried out. Each group of fish population is bred according to preset illumination cycle, monitors fish population reproductive hormone level change, analyzes the influence of illumination on fish population reproductive hormone, and formulates illumination control scheme. After the implementation of the scheme, the breeding time of carp is predicted, and the breeding time prediction data is obtained, and the efficiency of the control scheme is evaluated with the breeding cycle data. The evaluation result is used to adjust the scheme, to improve the efficiency of carp yield increasing breeding. The present application makes full use of photoperiod regulation factor, fine management carp growth and reproduction process, improves the efficiency and yield of aquaculture, reduces the cost, has important practical value and economic benefit.

The present application relates to the technical field of biological medicine, and particularly relates to a DNA vaccine for carp herpesvirus type II, a preparation method and application thereof. The present application provides a DNA vaccine for carp herpesvirus type II, which comprises a carrier and a nucleic acid molecule of an ORF66 gene, wherein the nucleic acid sequence of the ORF66 gene is shown as SEQ ID NO:1, and the carrier is pVAX1. The DNA vaccine for carp herpesvirus type II prepared by the present application is safer and more reliable, has good immunoprotective property, can inhibit the infection of carp herpesvirus type II on crucian carp, and reduce the economic loss caused by diseases in the process of crucian carp breeding.

The invention relates to the technical field of chafing dish soup base processing, in particular to a marmite thick soup health preserving chafing dish soup base preparation method which comprises the following steps: step 1, according to 1000kg of finished product soup base, putting 150kg of old hen, 200kg of pig bones and 100kg of duck bones into a multifunctional cutting and chopping machine with an ultrasonic cleaning function for cutting and cleaning, fishing out, then grabbing and mixing with 10kg of fresh ginger and 8kg of cooking wine, and pickling for 15 minutes, washing 50 kg of crucian carps and then drying the crucian carps; the raw materials are pretreated through ultrasonic waves and ice water, supercritical extraction is combined, solvent residues are avoided while flavor precursors of the raw materials are reserved, three-section temperature-controlled enzymolysis and Maillard reaction linkage is matched, umami substances are accurately released, and composite fragrance is generated; the rapid cooling and UHT instantaneous sterilization are combined with microbial control and nutrition retention, so that the product is rich in flavor, rich in layer and comprehensive in nutrition retention, and the flavor complexity, the nutrition retention rate and the production efficiency of the soup base are remarkably improved.

The invention belongs to the technical field of animal-derived active polypeptides, and discloses a He-prescription crucian carp skin collagen peptide with anti-osteoarthritis activity and application thereof. After being subjected to in-vitro simulated digestion, the He-prescription crucian carp skin collagen peptide comprises 10 peptide fragments which are respectively LVGPPGLT, IGMPGMT, LIGPPGL, NIGMPGM, IGPGPI, LTGFPGAA, GFNGLPGS, MTGPIGL, VGPPGAP and GLPGLAGR. The invention also discloses that the combined-prescription crucian skin collagen peptide has anti-osteoarthritis activity, can be further used for preparing anti-osteoarthritis products, can obviously relieve hyperalgesia behaviors and anxiety depression behaviors caused by osteoarthritis of mice, and can obviously improve the defects of rough surface, serious abrasion and poor anti-osteoarthritis of knee joint cartilage of mice caused by osteoarthritis. The number of cartilage cells is reduced, and joint matrix protein is lost.

The application discloses a Chuzhou crucian carp anti-aeromonas hydrophila ability prediction method and system based on data fusion, which comprises collecting physiological and biochemical, immune-related gene expression and post-infection phenotype multi-source heterogeneous data, and screening a characteristic variable set through alignment processing; an adaptive weighted fusion strategy is used to generate a fusion feature matrix, which is input into an improved deep learning model to train a preliminary prediction model; model parameters are optimized and a decision boundary is corrected through cross-validation to obtain an optimal prediction model; and the prediction data is input into the model after the same processing, and a quantitative result is output. In the method, the adaptive weighted fusion strategy realizes deep integration of data through feature extraction, redundancy elimination and weight optimization, the improved deep learning model adopts a hybrid network architecture and introduces an attention mechanism, and cross-validation is used to train and verify multiple groups of samples to optimize parameters, so that the prediction accuracy and generalization ability are effectively improved, and an efficient technical means is provided for evaluation of the stress resistance of Chuzhou crucian carp.

The present application relates to a kind of goldfish milt cell line and its application, belong to aquatic animal cell technical field.The preservation number of the present application goldfish milt cell line RcGFSB is: GDMCC NO:68000.The present application first successfully establishes the stable cell line from goldfish milt tissue, the cell line has good proliferation ability and is stable, can be long-term subculture and freeze storage;The goldfish milt cell line is sensitive to a variety of aquatic animal viruses, especially to the most serious virus pathogen of carp herpesvirus II type currently harmful to crucian carp and its variety, provides key experimental material for the isolation culture of the specificity virus and other aquatic pathogens of crucian carp and its variety, pathogenesis research and antiviral drug screening;The cell line has good exogenous gene expression capacity, can be used as fish bioreactor for recombinant protein production and gene engineering vaccine research and development, has important application prospect.