184 results about "Sexual reproduction" patented technology

The invention discloses an artificial standard planting utilizing method of Akebia trifoliate, which comprises that selecting planting base, preparing, cultivating seed, transplanting, and managing in field. The invention utilizes Akebia trifoliate seed to process sexual reproduction or utilizes young plant to process asexual propagation as grafting or the like, thereby realizing artificial standard transplantation of Akebia trifoliate, it can transplant one time and collect several times, utilize the plant continuously, improve plant utilization, reduce cost and protect environment, with wide application.

The invention discloses a method for realizing sexual reproduction and seedling culture of bletilla under an imitated ecological condition, wherein the large-scale production of bletilla seedlings is realized by using bletilla seeds in a near natural environment, namely under the imitated ecological condition. The method comprises the following steps of artificial supplementary pollinating bletilla, collecting and storing bletilla seeds, building a sowing condition, sowing and early managing after the sowing, and nurturing bletilla seedlings. By the method disclosed by the invention, the problem that the setting percentage of wild bletilla is low is solved, the sexual reproduction of the bletilla is performed under the imitated ecological condition which is built through imitating the nature; the survival rate and the growth density of the reproduced seedlings are high; the scale operation can be realized; the method is easily popularized and applied.

The invention relates to a breeding method of algae and particularly relates to a breeding method of sargassum vachellianum artificial seeds. The breeding method comprises the following steps: temporarily culturing adult algae of sargassum vachellianum in a culture solution for 18-22 days under the condition that the temperature is 16-20 DEG C, and replacing one half of the culture solution every 5-8 days till the appearance of female and male reproductive organs; charging gas for culturing for 1-5 days according to the proportion that the quantity of the female reproductive organs to the quantity of the male reproductive organs is 1: 1, and replacing one half of the culture solution every 1-3 days till fertilized eggs begin to fall off and settle; and collecting the fertilized eggs which begin to fall off and settle, standing and culturing for 18-25 days under the conditions that the temperature is 21-24 DEG C, the light intensity is 1800-2200lx and the lighting cycle is 14L: 10D. According to the method, the sexual reproduction time can be effectively controlled and prolonged, the higher fertilization rate and the survival rate of seedlings can be ensured, and the fertilization rate can be above 95% and are not affected by natural conditions and climate changes.

The invention belongs to the technical field of agriculture, relates to a sexual reproduction technology of plants, and particularly relates to a sexual reproduction method of gastrodia elata. The method sequentially comprises the nine steps of seed selection, germination accelerating, artificial pollination, fungus material preparation, seed dressing, sowing, transplantation of fungus materials for culture, cultivation and harvesting. By means of the sexual reproduction method of the gastrodia elata, the problems that in the existing gastrodia elata planting process, genetic depression occurs, the germination rate is low, seeds are prone to microbial contamination, the zero-generation quality is low, and the product yield is low are solved. In the cultivation process of the gastrodia elata, the microbial contamination rate is low, the growth vigor of target fungi is good, the germination rate is high, the zero-generation quality is good, high and stable yield is achieved, and the product quality is good.

The invention relates to a monitoring method for natural reproduction of fish based on environmental DNA technology. The method comprises the following steps: acquisition of a water sample; DNA extraction; ddPCR amplification of a target fragment; and concentration analysis. The monitoring method provided by the invention is based on environmental DNA identification technology, does not depend on traditional fishing technology for roe and fries and traditional anatomical technology for oophagous fish and can determine the oviposition behavior of a target species and the position of an oviposition site only through reasonable design of a sampling point, acquisition of a water sample and concentration analysis of the target species. The method maximally protects fish resources and avoids inconvenience caused by restrictive factors like long time for traditional fishing and difficulty in fishing of a part of fish fingerlings.

The invention discloses a preparation method of inactivated porcine parvovirus vaccine, comprising the following steps: (1) inoculating ST (swine testicle) cells with lentogen strains of porcine parvovirus, culturing to obtain production virus seeds; (2) adding treated microcarriers to a cell growth medium in a bioreactor, stirring; (3) inoculating the ST cells into the microcarriers in the bioreactor for cell reproduction and culturing; (4) infecting the suspension cultured ST cells in the bioreactor with the production virus seeds of the porcine parvovirus for virus reproduction and culturing; and (5) gathering the reproduced virus liquid, inactivating and preparing the vaccine. The method provided by the invention has long cell hold time, high cell viability and high density of living cells, and is favorable for continuous reproduction of virus, so that the prepared virus liquid has high virus titer. The production processes of the method disclosed by the invention are tightly and smoothly linked, the production scale is easy to enlarge, the porcine parvovirus is convenient to gather and stable in quality, and the method can obviously lower the production cost and effectively improve the yield and quality of the vaccine.

The invention discloses a seedling producing technique for rat-tailed algae, applying to seedling breed in-door according to sexual reproduction law, including: selecting and collecting the seed algae, stimulating and diffusing the seed algae, collecting the juvenile sporophyte, culturing and catadromousing seedlings, provided an artificial seedling producing technique for rat-tailed algae, dissolving the current shortcoming problem of the rat-tailed algae seedlings.

The invention provides a hibernating conservation asexual reproduction method for jellyfish hydranth. The method is characterized in that when normal cultivation of young jellyfish is finished in early summer every year, an enough quantity of jellyfish intestinal-shape young fingerlings are purchased and subjected to conservation, illumination, feeding, trace-amount inflation, water changing, asexual reproduction and development under the condition of low temperature. The method improves the efficiency and benefit of cultivating jellyfish fingerlings by reducing or avoiding a sexual reproduction process of fingerling cultivation, has a simple structure, reduces production cost, and is suitable for extensive popularization.

The invention discloses a greenhouse direct-seeding reproduction method for dendrobium officinale. According to the method, dendrobium officinale capsules are used as materials, at first, special phytohormone is used for treating dendrobium officinale seeds, a specific direct-seeding matrix is selected, the emergence rate of the dendrobium officinale seeds directly sown is 80% or above, and then by selecting a specific transplanting matrix, the survival rate of transplanted dendrobium officinale seedlings is 100% nearly. The method is simple, easy to implement and low in cost, the seedlings emerge fast, the seedling emergency rate is high, transplanted plants grow fast, the whole seedling culture period is short, the method is suitable for factorized rapid reproduction and large-scale field production, the requirement for planting dendrobium officinale in a large-area and large-scale mode can be met, a new path is opened up for rapid sexual reproduction of dendrobium officinale, and the method has double significance in production and economy.

The present invention provides a sargassum thunbergii seed-vegetable artificial-ripening condition controlling method, which can resolve the problems that the maturation of the male seed vegetable and the female seed vegetable is not synchronous and the developing time of the homosexual seed sargassum thunbergii in the sexual reproduction breeding process. The present invention uses the conditions that can be artificially controlled to achieve the artificial ripening of the seed vegetable in the seedling breeding process and can provide an excellent-quality seed vegetable for the seedling of the sargassum thunbergii. The technical proposal of the present invention comprises the selection of the adult sargassum thunbergii, gripping of the adult sargassum thunbergii, the selection of the temporary-cultivation pool and the controlling of the conditions for cultivation. Adopting the method of the present invention, the lighting time on the adult sargassum thunbergii is prolonged, the lighting strength is increased, the influence of the severe environment on the frond is reduced, the maturation of the pyrenium is relatively concentrated, the maturation of the male and the female pyrenium becomes synchronous and a plenty of concentrated fertilized eggs are achieved, which is beneficial for the success of the one-off seedling collection.

The invention relates to a nutrient solution capable of improving seed quality of rhizoma Gastrodiae. Each 1000 mL of the nutrient solution contains 10 to 15 g of sugar, 5.0 to 5.5 mg of ferrous sulfate heptahydrate, 11 to 13 mg of boric acid, 90 to 100 mg of ammonium chloride, 7.5 to 8.2 mg of copper sulphate, 22 to 25 mg of manganese sulfate, 12 to 15 mg of zinc sulfate and 30 to 50 ml of armillaria mellea fermentation broth. The nutrient solution can substantially improve the fruit setting rate and quality of seeds of rhizoma Gastrodiae, provides an excellent seed source for stable high yielding of sexual reproduction and improves economic benefits of production of rhizoma Gastrodiae.

The invention relates to a seedling breeding method using asparagus spores. The method comprises the steps of (1) spore collection, (2) indoor cultivation and (3) sea area hanging cultivation and period control. The seedling breeding method using the asparagus spores has the advantages that provenances needed by large-area popularization can be obtained effectively; indoor storage of asparagus during summer is achieved; seedling breeding is high in stability and strong in controllability; cost and labor and material consumption caused by north-to-south transfer and south-to-north transfer are effectively reduced; compared with vegetative propagation, generative propagation of seedlings has the advantages of being capable of breeding a large number of seedlings with different genetic backgrounds, selectively cultivating seedlings adaptable to different sea areas, and easing idioplasm degeneration to a certain degree; improved variety screening during asparagus breeding work is accelerated.

The present invention relates to sexual reproduction of plant containing tripterine, and is especially seed reproduction process of celastraceae plant containing tripterine. The seed reproduction process including seed treatment, young seedling culture, small seedling culture and field planting in certain conditions is favorable to the growth of root system and the synthesis and accumulation of tripterine. The present invention provides artificial culture technology of plant containing tripterine, and can improve environment while developing wild plant resource.

The present invention provides a molecular mechanism for gene containment in sexually reproducing transgenic plants. The mechanism is achieved with a molecular construct comprising a blocking construct (BC) that is inserted fully or partially into an intron of a transgene of interest (TGI). The TGI encodes desired gene products, such as heterologous or homologous proteins, peptides or other useful products. The expression of the BC leads to block of at least one molecular or physiological function that is essential for development or reproduction of the transgenic plant. Thereby the BC expression leads to death or incapacity of sexual reproduction of the plant. Moreover, the mechanism comprises an externally applicable recovering tool to recover the functions blocked by the BC. The recovering tool may be a recovering construct (RC).

Compositions and methods for producing a plant population lacking sexually derived embryos are provided. Compositions include suppression cassettes encoding polynucleotides and promoters resulting in parthenogenesis. Further provided are parthenogenesis genetic elements used to prevent sexual reproduction in self-reproducing plants.

Methods include: utilizing maternal embryo defective recessive mutations which are maintained as a sterile inbred maintenance system, allowing generation of populations that are homozygous for recessive mutant alleles, but transgenically complemented. Methods include utilizing a toxin genes expressed via egg-cell specific promoters, creating a dominant, embryo-less phenotypes, non-transmittable through female gametes. Resultant hemizygous plants are transformed with egg-cell promoters driving the antidote, a pollen ablation PTU and a seed color marker for identification of transgenic seed. The generation of a plants 50% female fertile, having seed which when grown in the next generation will yield plants with 50% viable transgenic seed, and 50% non-viable embryo-less seed.

The invention discloses a polyporus sexual propagation technique and culture method thereof, comprising: 1, spore seedbed culture method: selecting suitable seedbed ground and hacking wood-blocks as fungus substrate, digging pits, scattering the shoestring fungus on wood-blocks and covering soil or rotten plants on the shoestring fungus, selecting good hypha to sow, timely collecting sporophore spores, shredding and broadcasting the sporophore on the fungus substrate, heeling the fungus substrate using rotten plant fine earth tree-leaves, artificial controlling temperature; 2, spore sexual propagation method, (1) wooden case and bamboo basket cultivation method, sawing the fungus substrate into short segments and planting the polyporus in the short segments using the same culture method with the pit culture method, artificial controlling temperature; (2) soiless culture method, culturing the polyporus using soiless pure sand in river using the same culture method with the pit culture method, the inoculation speed of mycelitha and the shoestring fungus is quick and the growth speed is fast; the sexual propagation organs-sporophore of polyporus is directly used, thus 'polyporus flower' is used as strain source, namely the spored on the polyporus flower is used for propagating with strong growing ability and higher yield.

The invention discloses a method of sexual reproduction of radix psuedostellariae, which comprises the following steps that (1) a single plant is selected; (2) fruits are collected; (3) seeds are manually treated; (4) dormancy of the seeds is released; (5) land is prepared; (6) the seeds are sown, wherein the seeds are covered by fine soil, the thickness of the covered soil is 0 to 8 cm, and the soil on the box side is compacted; (7) field management is performed; and (8) the seeds are timely collected. According to the method, firstly, the good single plant and the seeds of the radix psuedostellariae are selected, so that the viability of the seeds reaches more than 94.49%, and the moisture content and the storage temperature and humidity of the seeds are controlled, so that the germination rate of the seeds of the radix psuedostellariae reaches more than 89.17%. In addition, the reasonable thickness of the covering soil is determined, so that the emergence rate of the radix pseudostellariae plant reaches 63.58%, the root dry weight per square meter reaches more than 198.83 g, and the reasonable collecting time is controlled, so that the quality and the yield of the medicine are guaranteed.