185 results about "Sexual reproduction" patented technology

The invention discloses an imitative wild sexual reproduction and breeding technique of gastrodia tuber. Main steps comprises that breeding is carried out to get mature gastrodia tuber seeds and good sprouting strain, mature class III shoestring fungus is adopted as production seed, which is planted under a natural hillside forest that is with over 80 percent of shade density and in which wild gastrodia tuber grows and is distributed; artificial mixed germination strain is adopted; room temperature is controlled so as to produce germination strain with seed; sexual reproduction, breeding and planting time is selected between June and August; the breeding technique is also suitable for field breeding. The invention has the advantages that: 1. bad factors resistance property of sexual reproduction and breeding of gastrodia tuber is improved. 2. Gastrodia tuber seeds are germinated to lead the growth cycle of protocorms to be a time slice that is basically matched with an animated period of the shoestring fungus; inoculation rate and survival rate of the protocorms are increased. 3. Raw materials are obtained locally, thus reducing cost; sustainable development of non-pollution gastrodia tuber planting industry is promoted in ecological agriculture forests. 4. The small site breeding method enhances competitor infection resistance of the gastrodia tuber seed.

The present invention concerns the use of chromosomal replacement techniques in the context of producing cloned and transgenic animals, in order to correct chromosome abnormalities or alter autosomal genotypes, and provide for novel breeding pairs by replacing the sex chromosome in animals to be cloned. Replacement of a sex chromosome, or an X or Y chromosome, will result in animals that are autosomally isogenic and sexually non-isogenic (AISN), with "autosomally isogenic" meaning that the paired sets of autosomes (non-sex chromosomes) in each animal are isogenic or identical. Also included in the invention are animals that are both "autosomally" and "allelically" isogenic whereby each particular pair of chromosomes is internally isogenic or identical within a single animal as well as between animals. Such animals are particularly useful in generating a line of cloned mammals using sexual reproduction, without having to undergo nuclear transfer in order to propagate cloned animals.

The invention discloses an artificial standard planting utilizing method of Akebia trifoliate, which comprises that selecting planting base, preparing, cultivating seed, transplanting, and managing in field. The invention utilizes Akebia trifoliate seed to process sexual reproduction or utilizes young plant to process asexual propagation as grafting or the like, thereby realizing artificial standard transplantation of Akebia trifoliate, it can transplant one time and collect several times, utilize the plant continuously, improve plant utilization, reduce cost and protect environment, with wide application.

The invention relates to a molecular detection method for rapidly distinguishing MAT1-1 and MAT1-2 mating types of Chinese caterpillar fungus, comprising two pairs of specific primers (Cm1-F / Cm1-R andCm2-F / Cm2-R) for detecting mating types and two sets of corresponding PCR amplification systems and PCR amplification programs. The primer pair Cm1-F / Cm1-R is used for detecting the MAT1-1 mating type of Chinese caterpillar fungus, and the other primer pair Cm2-F / Cm2-R is used for detecting the MAT1-2 mating type of Chinese caterpillar fungus; and using both pairs of the specific primers and thecorresponding PCR amplification systems and the PCR amplification programs can realize the rapid detection of the mating types of Chinese caterpillar fungus. The detection results of the molecular detection method have high specificity, and the molecular detection method is stable, reliable and rapid and can greatly shorten the time of biological detection. The molecular detection method is suitable for the detection of the mating types of Chinese caterpillar fungus in the relevant professional fields such as production of edible fungi and medicinal fungi, strain breeding, plant protection andthe like as well as the research in the aspects of mating type gene, phylogenetic development and the like, and has significance in researching sexual reproduction of other fungi of cordyceps and directing the production practice.

The invention discloses a gene for controlling male reproduction and development of rice and application of the gene. The gene has a nucleotide sequence represented by SEQ ID NO.1, and a protein encoded by the gene has an amino acid sequence represented by SEQ ID NO.2. By targeting a LOC_Os08g20730 gene design target by virtue of a CRISPR / Cas9 gene editing system, a receptor is a middle flower 11, and a targeted T0 generation phenotype are shown in the figures 1, 2, 3 and 4 in the description; and sequencing analysis shows that anther development abnormality occurs in a homozygously mutated plant or a biallelically mutated plant and pollen does not exist. The analysis of a homozygously mutated T1 generation shows that the separation proportion of fertile plants to sterile plants is 3 to 1. The integration shows that the gene is capable of regulating and controlling the reproduction and development of rice, once the gene in the rice is damaged, the anther development abnormality occurs, and non-pollen sterility occurs. According to the obtained cell nucleus gene for controlling the reproduction and development of the rice, an intelligent sterile line can be created by virtue of a molecular measure, and excellent parents in the production are improved into excellent sterile lines, so that the heterosis utilization is expanded.

The invention provides a preparation method of a non-transgenic mutant based on a CRISPR-Cas9 technology. Specifically, the preparation method comprises a construction method and a screening method. The construction method is characterized in that CRISPR-Cas9 and sgRNA are preassembled and are located at T-DNA of agrobacterium tumefaciens; site-directed change of a target gene of a target plant can be realized by infection of the target plant by the agrobacterium tumefaciens and coculture without integrating the sequences of the CRISPR-Cas9 and the sgRNA into a genome of the target plant, so that an obtained site-directed mutant material of the target gene does not need the processes of sexual reproduction, segregation posterity, population screening and the like or does not contain any exogenous gene sequence. The obtained regeneration seedlings are screened by a high-throughput sequencing and high-resolution melting curve technology provided by the invention; mutant plants of which target genes are mutated can be obtained by identifying the regeneration seedlings efficiently and quickly at the current generation of transgene, even if low-proportion mutants of which the proportionis 1 / 100 of a mixed population can be screened. The screening method provided by the invention still can ensure excellent accuracy and sensitivity.

The invention discloses a method for realizing sexual reproduction and seedling culture of bletilla under an imitated ecological condition, wherein the large-scale production of bletilla seedlings is realized by using bletilla seeds in a near natural environment, namely under the imitated ecological condition. The method comprises the following steps of artificial supplementary pollinating bletilla, collecting and storing bletilla seeds, building a sowing condition, sowing and early managing after the sowing, and nurturing bletilla seedlings. By the method disclosed by the invention, the problem that the setting percentage of wild bletilla is low is solved, the sexual reproduction of the bletilla is performed under the imitated ecological condition which is built through imitating the nature; the survival rate and the growth density of the reproduced seedlings are high; the scale operation can be realized; the method is easily popularized and applied.

An artificial reproduction method for drynaria includes asexual production and spore reproduction. Said asexual reproduction includes such steps as taking its rhizome segment with 3-5 bud points and 3-5 sporophyllary leaves, fixing it to tree or mountain rock, covering it by bryophyte, spraying water and management. Said spore reproduction includes such steps as sterilizing the burnt earth, immersing in cold boiled water, loading the sporanges in bag, drying in air, preparing the suspension of sporanges, spraying it onto said burnt earth, optical culture to obtain gametophytes, transplanting, culturing to obtain sporophytes, and transplanting them on tree or mountain rock.

The invention provides a Daphnia reproductive bioassay for detecting and confirming the presence of a toxic substance in an aqueous sample, and / or for screening the substance as an endocrine disrupter. According to the assay, a test sample is brought into contact with adult, oviporous Daphnia of a single clone under conditions of crowding and growth conditions to stimulate sexual reproduction and the production of males. The bioassay is based upon the measurement of endpoints that convey quantitative information about the biological activity of the substance: survivorship, numbers of female offspring, numbers of male offspring, number of resting eggs, number of offspring that display developmental deformities or behavioral abnormalities, and nutritional status of the offspring. Also provided are kits for use in conducting the bioassay.

The invention discloses a wild culture-imitating method for rheum officinale baill. The method is characterized by planting seedlings subjected to sexual reproduction and seed blocks subjected to asexual reproduction in an adret sloping field with a high altitude of 1,500 to 1,800 m and a gradient of less than or equal to 30 degrees and performing extensive management by a wild-imitating method, wherein the seedlings subjected to sexual reproduction is cultured in an adret flat field with a low altitude of 800 to 1,000 m; during seedling culture, the thousand seed weight is more than or equal to 12.9 g, the moisture content is lower than 6.51 percent and the purity is over 98.9 percent; after immersion and germination acceleration, the seeds are sown on a seedbed; after the seedlings grow to be 10 centimeters high and the ground diameter is more than 2 centimeters, the seedlings are transplanted; the transplanting time is the first ten days of April every year; farmyard manure subjected to innocent treatment is applied one time per year; and after three years, the rheum officinale baill can be harvested. The invention provides a practicable method for realizing large-area standard plantation of the rheum officinale baill. By the method, the yield and the quality of the rheum officinale baill can be improved; and all the officinal active ingredients of the product are over 1.5 percent.

The invention relates to a breeding method of algae and particularly relates to a breeding method of sargassum vachellianum artificial seeds. The breeding method comprises the following steps: temporarily culturing adult algae of sargassum vachellianum in a culture solution for 18-22 days under the condition that the temperature is 16-20 DEG C, and replacing one half of the culture solution every 5-8 days till the appearance of female and male reproductive organs; charging gas for culturing for 1-5 days according to the proportion that the quantity of the female reproductive organs to the quantity of the male reproductive organs is 1: 1, and replacing one half of the culture solution every 1-3 days till fertilized eggs begin to fall off and settle; and collecting the fertilized eggs which begin to fall off and settle, standing and culturing for 18-25 days under the conditions that the temperature is 21-24 DEG C, the light intensity is 1800-2200lx and the lighting cycle is 14L: 10D. According to the method, the sexual reproduction time can be effectively controlled and prolonged, the higher fertilization rate and the survival rate of seedlings can be ensured, and the fertilization rate can be above 95% and are not affected by natural conditions and climate changes.

The invention belongs to the technical field of agriculture, relates to a sexual reproduction technology of plants, and particularly relates to a sexual reproduction method of gastrodia elata. The method sequentially comprises the nine steps of seed selection, germination accelerating, artificial pollination, fungus material preparation, seed dressing, sowing, transplantation of fungus materials for culture, cultivation and harvesting. By means of the sexual reproduction method of the gastrodia elata, the problems that in the existing gastrodia elata planting process, genetic depression occurs, the germination rate is low, seeds are prone to microbial contamination, the zero-generation quality is low, and the product yield is low are solved. In the cultivation process of the gastrodia elata, the microbial contamination rate is low, the growth vigor of target fungi is good, the germination rate is high, the zero-generation quality is good, high and stable yield is achieved, and the product quality is good.

The invention relates to a monitoring method for natural reproduction of fish based on environmental DNA technology. The method comprises the following steps: acquisition of a water sample; DNA extraction; ddPCR amplification of a target fragment; and concentration analysis. The monitoring method provided by the invention is based on environmental DNA identification technology, does not depend on traditional fishing technology for roe and fries and traditional anatomical technology for oophagous fish and can determine the oviposition behavior of a target species and the position of an oviposition site only through reasonable design of a sampling point, acquisition of a water sample and concentration analysis of the target species. The method maximally protects fish resources and avoids inconvenience caused by restrictive factors like long time for traditional fishing and difficulty in fishing of a part of fish fingerlings.

The invention discloses a preparation method of inactivated porcine parvovirus vaccine, comprising the following steps: (1) inoculating ST (swine testicle) cells with lentogen strains of porcine parvovirus, culturing to obtain production virus seeds; (2) adding treated microcarriers to a cell growth medium in a bioreactor, stirring; (3) inoculating the ST cells into the microcarriers in the bioreactor for cell reproduction and culturing; (4) infecting the suspension cultured ST cells in the bioreactor with the production virus seeds of the porcine parvovirus for virus reproduction and culturing; and (5) gathering the reproduced virus liquid, inactivating and preparing the vaccine. The method provided by the invention has long cell hold time, high cell viability and high density of living cells, and is favorable for continuous reproduction of virus, so that the prepared virus liquid has high virus titer. The production processes of the method disclosed by the invention are tightly and smoothly linked, the production scale is easy to enlarge, the porcine parvovirus is convenient to gather and stable in quality, and the method can obviously lower the production cost and effectively improve the yield and quality of the vaccine.

The invention provides a jelly fish short-term low-temperature induction rearing method. The method is characterized in that: a strong jelly fish scyphistoma is prepared about a month before jelly fish breeding, is bred for a period at low temperature in an environment that natural water temperature is gradually cooled to low temperature, and then is heated to strobilation induction temperature; and operations of water changing, bait casting, air charge and lighting are carried out, so the jelly fish acetabular seedlings can be obtained in batch in short term. The jelly fish breeding method is not restricted by seasons, can breed the jelly fish acetabular larva in short term after the jelly scyphistoma is obtained, can shorten or avoid the sexual reproduction process of breeding to a certain extent, can improve the efficiency and benefit of jelly fish breeding, has concise process, lowers production cost, and is suitable for wide popularization.

The invention discloses an Eimeria tenella AMA 1 gene, the nucleotide sequence of which includes a DNA sequence that codes the amino acid sequence represented by SEQ ID No.1 or a DNA sequence that codes the amino acid sequence which has AMA 1 protein activity and is obtained by omitting, adding, inserting or substituting one or more amino acid in the amino acid sequence represented by SEQ ID No.1. The invention also discloses proteins coded by the above-mentioned genes. The Eimeria tenella AMA 1 gene and proteins provided in the invention are closely related to invasion of daughter spores, escape and invasion of merozoites and escape and invasion of microgametes in the stage of sexual reproduction, and are suitable for being developed into novel vaccines or medicines for controlling coccidiosis.

The invention discloses a seedling producing technique for rat-tailed algae, applying to seedling breed in-door according to sexual reproduction law, including: selecting and collecting the seed algae, stimulating and diffusing the seed algae, collecting the juvenile sporophyte, culturing and catadromousing seedlings, provided an artificial seedling producing technique for rat-tailed algae, dissolving the current shortcoming problem of the rat-tailed algae seedlings.

The invention belongs to the technical field of sexual reproduction of plants and discloses a method of raising seedlings for Lonicera sempervirens seed. The method mainly comprises following steps: fruit harvesting; seed after-ripening and storing; seed soaking; seed pregermination; seed sowing; seedling potting, etc. According to the method, the time of after-ripening of Lonicera sempervirens seed is increased; low temperature treatment facilitates breaking of seed dormancy; Lonicera sempervirens seed germination is accelerated due to suitable temperature so that the time of seedling emergence is reduced. The method has following beneficial effects: tidiness of seedling emerging; simplicity of operation during seedling; ease of implementation. The method provides forceful foundation for breeding seeds and raising seedlings for Lonicera sempervirens. Commercialization application and promotion of the technology are facilitated.

The invention provides a hibernating conservation asexual reproduction method for jellyfish hydranth. The method is characterized in that when normal cultivation of young jellyfish is finished in early summer every year, an enough quantity of jellyfish intestinal-shape young fingerlings are purchased and subjected to conservation, illumination, feeding, trace-amount inflation, water changing, asexual reproduction and development under the condition of low temperature. The method improves the efficiency and benefit of cultivating jellyfish fingerlings by reducing or avoiding a sexual reproduction process of fingerling cultivation, has a simple structure, reduces production cost, and is suitable for extensive popularization.

The invention discloses a greenhouse direct-seeding reproduction method for dendrobium officinale. According to the method, dendrobium officinale capsules are used as materials, at first, special phytohormone is used for treating dendrobium officinale seeds, a specific direct-seeding matrix is selected, the emergence rate of the dendrobium officinale seeds directly sown is 80% or above, and then by selecting a specific transplanting matrix, the survival rate of transplanted dendrobium officinale seedlings is 100% nearly. The method is simple, easy to implement and low in cost, the seedlings emerge fast, the seedling emergency rate is high, transplanted plants grow fast, the whole seedling culture period is short, the method is suitable for factorized rapid reproduction and large-scale field production, the requirement for planting dendrobium officinale in a large-area and large-scale mode can be met, a new path is opened up for rapid sexual reproduction of dendrobium officinale, and the method has double significance in production and economy.

A method provided by the present invention is applicable to the aspects such as sargassum fusiform germplasm resource analysis, artificially cultured sargassum fusiform group line classification, elite seed and pedigree seed near-source genetic relationship analysis and manual seedling screening by using sexual reproduction of sargassum fusiform. The method mainly comprises the operating steps of sargassum fusiform history feature and sargassum fusiform mature sporophyte morphological feature main component morphological measurement, sargassum fusiform mature sporophyte morphological feature correlation mathematical model analysis, sargassum fusiform mature sporophyte morphological feature main component mathematical model analysis, sargassum fusiform mature sporophyte morphological feature main component cluster mathematical model analysis, and sargassum fusiform mature sporophyte line differentiation and classification determination and the like. The present invention may provide a scientific differentiation and classification method for the aspects such as sargassum fusiform germplasm resource investigation, sargassum fusiform mature sporophyte group diversity analysis, sargassum basic scientific research and experimental sample screening, sargassum fusiform introduced seed morphological feature stability analysis, manual elite seed differentiation and screening by using sexual reproduction of sargassum fusiform and asexual reproduction excellent plant rhizoid storage.

The present invention provides a sargassum thunbergii seed-vegetable artificial-ripening condition controlling method, which can resolve the problems that the maturation of the male seed vegetable and the female seed vegetable is not synchronous and the developing time of the homosexual seed sargassum thunbergii in the sexual reproduction breeding process. The present invention uses the conditions that can be artificially controlled to achieve the artificial ripening of the seed vegetable in the seedling breeding process and can provide an excellent-quality seed vegetable for the seedling of the sargassum thunbergii. The technical proposal of the present invention comprises the selection of the adult sargassum thunbergii, gripping of the adult sargassum thunbergii, the selection of the temporary-cultivation pool and the controlling of the conditions for cultivation. Adopting the method of the present invention, the lighting time on the adult sargassum thunbergii is prolonged, the lighting strength is increased, the influence of the severe environment on the frond is reduced, the maturation of the pyrenium is relatively concentrated, the maturation of the male and the female pyrenium becomes synchronous and a plenty of concentrated fertilized eggs are achieved, which is beneficial for the success of the one-off seedling collection.

The invention relates to a nutrient solution capable of improving seed quality of rhizoma Gastrodiae. Each 1000 mL of the nutrient solution contains 10 to 15 g of sugar, 5.0 to 5.5 mg of ferrous sulfate heptahydrate, 11 to 13 mg of boric acid, 90 to 100 mg of ammonium chloride, 7.5 to 8.2 mg of copper sulphate, 22 to 25 mg of manganese sulfate, 12 to 15 mg of zinc sulfate and 30 to 50 ml of armillaria mellea fermentation broth. The nutrient solution can substantially improve the fruit setting rate and quality of seeds of rhizoma Gastrodiae, provides an excellent seed source for stable high yielding of sexual reproduction and improves economic benefits of production of rhizoma Gastrodiae.

The invention relates to a seedling breeding method using asparagus spores. The method comprises the steps of (1) spore collection, (2) indoor cultivation and (3) sea area hanging cultivation and period control. The seedling breeding method using the asparagus spores has the advantages that provenances needed by large-area popularization can be obtained effectively; indoor storage of asparagus during summer is achieved; seedling breeding is high in stability and strong in controllability; cost and labor and material consumption caused by north-to-south transfer and south-to-north transfer are effectively reduced; compared with vegetative propagation, generative propagation of seedlings has the advantages of being capable of breeding a large number of seedlings with different genetic backgrounds, selectively cultivating seedlings adaptable to different sea areas, and easing idioplasm degeneration to a certain degree; improved variety screening during asparagus breeding work is accelerated.

The present invention relates to sexual reproduction of plant containing tripterine, and is especially seed reproduction process of celastraceae plant containing tripterine. The seed reproduction process including seed treatment, young seedling culture, small seedling culture and field planting in certain conditions is favorable to the growth of root system and the synthesis and accumulation of tripterine. The present invention provides artificial culture technology of plant containing tripterine, and can improve environment while developing wild plant resource.

The present invention provides a molecular mechanism for gene containment in sexually reproducing transgenic plants. The mechanism is achieved with a molecular construct comprising a blocking construct (BC) that is inserted fully or partially into an intron of a transgene of interest (TGI). The TGI encodes desired gene products, such as heterologous or homologous proteins, peptides or other useful products. The expression of the BC leads to block of at least one molecular or physiological function that is essential for development or reproduction of the transgenic plant. Thereby the BC expression leads to death or incapacity of sexual reproduction of the plant. Moreover, the mechanism comprises an externally applicable recovering tool to recover the functions blocked by the BC. The recovering tool may be a recovering construct (RC).

Compositions and methods for producing a plant population lacking sexually derived embryos are provided. Compositions include suppression cassettes encoding polynucleotides and promoters resulting in parthenogenesis. Further provided are parthenogenesis genetic elements used to prevent sexual reproduction in self-reproducing plants.Methods include: utilizing maternal embryo defective recessive mutations which are maintained as a sterile inbred maintenance system, allowing generation of populations that are homozygous for recessive mutant alleles, but transgenically complemented. Methods include utilizing a toxin genes expressed via egg-cell specific promoters, creating a dominant, embryo-less phenotypes, non-transmittable through female gametes. Resultant hemizygous plants are transformed with egg-cell promoters driving the antidote, a pollen ablation PTU and a seed color marker for identification of transgenic seed. The generation of a plants 50% female fertile, having seed which when grown in the next generation will yield plants with 50% viable transgenic seed, and 50% non-viable embryo-less seed.

The invention discloses a polyporus sexual propagation technique and culture method thereof, comprising: 1, spore seedbed culture method: selecting suitable seedbed ground and hacking wood-blocks as fungus substrate, digging pits, scattering the shoestring fungus on wood-blocks and covering soil or rotten plants on the shoestring fungus, selecting good hypha to sow, timely collecting sporophore spores, shredding and broadcasting the sporophore on the fungus substrate, heeling the fungus substrate using rotten plant fine earth tree-leaves, artificial controlling temperature; 2, spore sexual propagation method, (1) wooden case and bamboo basket cultivation method, sawing the fungus substrate into short segments and planting the polyporus in the short segments using the same culture method with the pit culture method, artificial controlling temperature; (2) soiless culture method, culturing the polyporus using soiless pure sand in river using the same culture method with the pit culture method, the inoculation speed of mycelitha and the shoestring fungus is quick and the growth speed is fast; the sexual propagation organs-sporophore of polyporus is directly used, thus 'polyporus flower' is used as strain source, namely the spored on the polyporus flower is used for propagating with strong growing ability and higher yield.

The invention discloses a method of sexual reproduction of radix psuedostellariae, which comprises the following steps that (1) a single plant is selected; (2) fruits are collected; (3) seeds are manually treated; (4) dormancy of the seeds is released; (5) land is prepared; (6) the seeds are sown, wherein the seeds are covered by fine soil, the thickness of the covered soil is 0 to 8 cm, and the soil on the box side is compacted; (7) field management is performed; and (8) the seeds are timely collected. According to the method, firstly, the good single plant and the seeds of the radix psuedostellariae are selected, so that the viability of the seeds reaches more than 94.49%, and the moisture content and the storage temperature and humidity of the seeds are controlled, so that the germination rate of the seeds of the radix psuedostellariae reaches more than 89.17%. In addition, the reasonable thickness of the covering soil is determined, so that the emergence rate of the radix pseudostellariae plant reaches 63.58%, the root dry weight per square meter reaches more than 198.83 g, and the reasonable collecting time is controlled, so that the quality and the yield of the medicine are guaranteed.