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Breeding method of sargassum vachellianum artificial seeds

A Sargasso varnishae, artificial technology, applied in the fields of botanical equipment and methods, seaweed cultivation, climate change adaptation, etc., can solve problems such as lack of research, achieve high seedling survival rate, control and prolong sexual reproduction time, avoid mutual adhesion effect

Inactive Publication Date: 2013-07-17
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there is almost no research on the reproductive biology and seed breeding methods of this type of seaweed. Therefore, it is of great significance to carry out artificial maturation of algae indoors, complete sexual reproduction, and obtain fertilized eggs for artificial seedling breeding.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0027] (1) Algae selection:

[0028] Sargassum varschii grown in the subtidal zone of the natural sea area, which has developed panicle-like reproductive receptacle prototypes, cannot distinguish between male and female individuals, has fewer epiphytes, and is in good health are used as algae.

[0029] (2) Temporary cultivation of algae:

[0030] The sterilized seawater used to grow algae is temporarily raised in a culture bucket with culture medium, and half of the seawater is replaced every 7 days for 20 days. Temporary breeding conditions: temperature 18°C, light intensity 1500-2000lx, light cycle 10L:14D.

[0031] (3) Reproductive care to promote maturation:

[0032] Cut off the side branches with genital receptacles from the temporarily raised algae in step (2), each side branch is 3-5cm long, and has 15-20 genital receptacles on it, according to the ratio of 1:1 between female and male genital receptacles, Inflate culture in the same inflatable bottle (use air pump to...

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Abstract

The invention relates to a breeding method of algae and particularly relates to a breeding method of sargassum vachellianum artificial seeds. The breeding method comprises the following steps: temporarily culturing adult algae of sargassum vachellianum in a culture solution for 18-22 days under the condition that the temperature is 16-20 DEG C, and replacing one half of the culture solution every 5-8 days till the appearance of female and male reproductive organs; charging gas for culturing for 1-5 days according to the proportion that the quantity of the female reproductive organs to the quantity of the male reproductive organs is 1: 1, and replacing one half of the culture solution every 1-3 days till fertilized eggs begin to fall off and settle; and collecting the fertilized eggs which begin to fall off and settle, standing and culturing for 18-25 days under the conditions that the temperature is 21-24 DEG C, the light intensity is 1800-2200lx and the lighting cycle is 14L: 10D. According to the method, the sexual reproduction time can be effectively controlled and prolonged, the higher fertilization rate and the survival rate of seedlings can be ensured, and the fertilization rate can be above 95% and are not affected by natural conditions and climate changes.

Description

technical field [0001] The invention relates to a breeding method for algae, in particular to a breeding method for artificial seedlings of Sargassum varschii. Background technique [0002] Sargassum belongs to Phaeophyta, Sargassumaceae. my country is one of the main producing areas of Sargassum, and there are more than 60 kinds of them. Common ones include Sargassum schistophora, Sargassum half-leaf, Sargassum stoloni, Sargassum Heinzii, Artemisia sargassum, and Sargassum waschii. Among them, Sargassum vachellianum Greville, Latin name Sargassum vachellianum Greville, is a species concentrated in the coastal areas of Zhejiang, Fujian, Guangdong and Hong Kong in my country. Sargassum vachellianum itself is rich in protein, carbohydrates, inorganic salts, vitamins, etc. It is widely used in the fields of medicine, food, feed and organic fertilizer. Especially in the field of organic fertilizers, it has attracted people's attention. Compared with chemical fertilizers, Sarga...

Claims

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Application Information

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IPC IPC(8): A01G33/00
CPCY02A40/80
Inventor 严兴洪张婧
Owner SHANGHAI OCEAN UNIV
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