5093 results about "Seedling" patented technology

The invention relates to the technical field of microorganisms, and particularly discloses a saline-alkaline tolerant bacillus deserticola BW-13 with biocontrol and growth promoting functions and application thereof. The bacillus safensis BW-13 is preserved in the China Center for Type Culture Collection on January 13, 2025, the classification name of the bacillus safensis BW-13 is bacillus safeensis, and the preservation number of the bacillus safensis BW-13 is CCTCC NO: M 2025108. The strain BW-13 has relatively high salt tolerance, and still keeps good resistance under various stress conditions; meanwhile, the plant growth promoting agent has various plant growth promoting characteristics including nitrogen fixation, phosphorus solubilization and cellulase and protease secretion capacity, and pot experiment verifies that the plant growth promoting agent can improve the salt resistance of cucumber seedlings and promote plant growth; the strain has an antagonistic effect on a plurality of phytopathogens, and especially has a remarkable prevention and treatment effect on plant diseases such as apple ring spot, polygonatum kingianum anthracnose, pear tree rot and tomato gray mold caused by ascomycophyta pathogenic fungi.

The invention discloses a method for evaluating enhancement and release seedling resources based on environmental DNA polymerization analysis. The method comprises the following steps: carrying out gridding partition on a target water area, collecting a water sample through a designed sampling scheme, and carrying out DNA extraction and high-throughput sequencing to obtain species sequence information of each sampling point. Sequencing data is subjected to species identification by using a bioinformatics method, released species are identified, a spatial abundance model is established, and a preliminary distribution map is generated. And establishing a DNA degradation kinetic model in combination with water area environmental parameters, and carrying out reverse correction on abundance distribution. And through a resource inversion model coupled with hydrodynamics, analyzing biomass distribution characteristics and migration laws of the release group, and obtaining a resource evaluation result. And finally, a species environment preference model is constructed based on migration path analysis, an optimal release area is matched in a target water area, a scientific scheme including release point locations, opportunities and quantity is generated, and a whole-process technical support and a decision basis are provided for enhancement and release.

The invention relates to the technical field of mountain afforestation, provides an efficient nursery stock planting method suitable for mountain afforestation, and aims at solving the problems that in existing mountain afforestation, planting is not standard, the survival rate is low, the water and soil conservation capacity is weak, and the labor intensity is high. The forest space layout is optimized, and the water and soil conservation capability is enhanced; the mellow soil and immature soil layered stacking and backfilling technology is adopted, the natural structure of the soil is recovered, and the root zone microenvironment quality is improved; a fertilizer isolation layer is arranged and degradable container seedlings are implanted, so that the risk of root burning is avoided, the integrity of root systems is protected, and the survival rate of the seedlings is remarkably increased; by combining root fixing water slow irrigation, water retention covering and slope water collection and soil conservation structures, natural rainfall is effectively utilized, evaporation and runoff are reduced, and water saving and drought resisting are achieved; the whole process flow is standard, the replicability is high, and the dependence on artificial experience is reduced.

The invention relates to the technical field of saline-alkali soil treatment, and discloses a light and moderate saline-alkali soil alfalfa seedling protection and strong growth promotion planting method which comprises the following steps: S1, soil pretreatment and root zone microenvironment construction; s2, seed treatment and space-time double-salt-avoiding sowing; s3, microbial inoculum synergistic application; s4, water and fertilizer integration and accurate management; s5, dynamic regulation and control in the growth period, a low-salt root zone microenvironment is constructed through combination of physical obstruction and biological improvement, salt ascending is inhibited by using a slag salt isolation layer, fertility is improved by using an organic layer, and stress resistance is enhanced by adopting a salt-tolerant variety, climate window sowing and aerogel embedding fungicide; precise regulation and control are achieved through water and fertilizer integrated drip irrigation, the stability of a root zone is maintained by combining intertillage and directional topdressing, finally, the seedling emergence uniformity and the seedling stage survival rate are remarkably improved, and robust growth of plants in saline-alkali soil is promoted.

The invention relates to the technical field of molecular markers, in particular to an Indel molecular marker related to yellow leaves of a melon seedling growing point and application of the Indel molecular marker. The invention explores a key candidate gene MELO3C016136 for controlling the leaf color of the growth point of the muskmelon seedling, and develops an Indel molecular marker ygp2 for identifying the leaf color change of the growth point of the muskmelon seedling based on the key candidate gene MELO3C016136. The molecular marker ygp2 can be used for identifying the leaf color change character of the growth point of the muskmelon seedling, only one-time PCR amplification and polyacrylamide gel electrophoresis need to be carried out on the DNA of the plant to be detected, the identification result is reliable, the genotype of the target plant can be simply and conveniently identified, and the genotype identification result is completely consistent with the leaf color phenotype of the growth point of the seedling; important technical support is provided for creating new muskmelon germplasm with yellow seedling growing point leaves.

The invention discloses a sporosarcina sp. Strain RH34 and an application of the sporosarcina sp. Strain RH34 and the application of the sporosarcina sp. Strain RH34. The sporosarcina strain RH34 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, yard 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is December 25, 2024, and the preservation number is CGMCC NO.33199. The sporosarcina strain RH34 can be used for preparing the sporosarcina strain RH34. The alfalfa rhizosphere microorganism sporosarcina strain RH34 is separated from alfalfa roots in salinized soil, and has the functions of dissolving organic phosphorus, dissolving inorganic phosphorus, producing biological membranes, producing siderophores, producing IAA and producing ACC deaminase. Seed soaking and germination under salt stress can obviously improve the germination index of alfalfa seeds. The growth condition of alfalfa seedlings can be improved under salt stress.

The invention relates to the technical field of shellfish breeding, in particular to a rapid microalgae breeding method for shellfish breeding based on a photobioreactor and a cement pool. The method comprises the following steps: firstly, optimizing a photobioreactor structure and an illumination layout, controlling a culture environment by matching with a multi-parameter regulation and control system, breeding microalgae seeds with high resistance and high carbon sequestration capacity, culturing to a preset density, and partially harvesting and inoculating into a cement pond, after partial harvest, the photobioreactor realizes continuous culture by supplementing disinfected seawater and nutritive salt; the harvested high-density microalgae are inoculated into a cement pond, nutritive salt is supplemented in a doubled mode, air and carbon dioxide mixed gas is used for continuous aerated culture, and the microalgae are promoted to rapidly reach the index growth period and used for being directly fed to shellfish larvae; according to the rapid microalgae breeding method for shellfish seedling culture, the yield and quality of microalgae are both considered, the success rate and productivity of aquatic seedling culture are effectively improved, the production cost and the labor intensity of workers are reduced, and the method is suitable for large-scale popularization.

The present application relates to a kind of methods for mass propagation of tea corner miridae in laboratory, comprising the following technical steps: collecting tea garden tea corner miridae adult, cultivating fall kui seedling, putting seedling and leading insect, tea corner miridae habitat and feeding, mating and oviposition and nymph hatching and feeding.The present application uses fall kui seedling as the main body of feeding raw material, and the leaf of raw material is relatively thick, strong in harm resistance, not easy to dry, which helps to improve the hatching rate of tea corner miridae eggs, reduce the frequency of raw material supply and replacement, at the same time, the present application saves time and effort, can be realized in laboratory low cost and sustainability fast, efficient, mass propagation of tea corner miridae test insect source.

The utility model discloses an artificial climate chamber water-cooling seedling raising vehicle, a chassis comprises a bottom frame, a support is connected to the bottom frame, and trundles are connected to the bottom of the bottom frame; the supporting pieces are connected to the corners of the bottom frame; the multiple groups of clamping strips are arranged up and down, each group comprises two clamping strips, heat dissipation holes are formed in the clamping strips, and the two ends of the clamping strips are connected to the supporting pieces; the light guide plate is clamped between each group of clamping strips, and light guide points are arranged on the light guide plate; the light source is inserted into the clamping strip and irradiates the side part of the light guide plate; the water inlet pipe is connected to the supporting piece, and one end of the heat dissipation hole of the clamping strip communicates with the water inlet pipe; the water return pipe is connected to the supporting piece, and the other end of the heat dissipation hole of the clamping strip communicates with the water return pipe. Compared with the prior art, the circulating water cooling system enables the seedling raising vehicle to keep a proper temperature environment, damage to seeds or seedlings caused by too high temperature is avoided, and seed germination and seedling growth are facilitated.

The invention relates to the technical field of molecular biology, and discloses 74 core molecular marker sets for identifying cold resistance of pennisetum alopecuroides, a primer group, a detection reagent, a kit, a gene chip and application thereof. According to the method, core germplasm resources of pennisetum alopecuroides are systematically collected, and the GWAS population is constructed by utilizing multi-environment (different altitudes) conditions, so that wide genetic and phenotypic variation is fully covered, and a representative material foundation is laid for cold-resistant genetic analysis. In the aspect of phenotype evaluation, a method for determining the number of reviving and root cutting seedlings after natural overwintering by combining single-root double-stem-node oblique cutting planting is innovatively provided, a set of scientific and stable cold resistance and reproducibility evaluation system capable of being operated in a standardized mode is established, and the reliability and efficiency of phenotype identification are remarkably improved. The detection is not influenced by human and climate environments; the method realizes efficient and accurate early cold resistance identification of pennisetum alopecuroides, greatly improves the breeding screening efficiency and scale, and accelerates the breeding process.

The invention discloses an upland cotton GhCRK29 gene as well as an encoding protein and application thereof, the GhCRK29 gene is separated from cotton, the nucleotide sequence of the GhCRK29 gene is as shown in SEQ ID No.1, and the amino acid sequence of the encoding protein of the GhCRK29 gene is as shown in SEQ ID No.2. According to the invention, a virus-induced gene silencing technology and a transgenic overexpression technology are further utilized to carry out preliminary research on the function of the cotton GhCRK29 gene in cotton verticillium wilt resistance, and a result shows that the verticillium wilt resistance of a cotton seedling plant is obviously reduced after the GhCRK29 gene in cotton is silenced; after the GhCRK29 gene is over-expressed in arabidopsis thaliana or cotton, the verticillium wilt resistance of arabidopsis thaliana or cotton is remarkably improved, and the resistance of a GhCRK29 gene forward regulation plant to verticillium dahliae is determined; the invention has application prospects in the aspects of improving the verticillium wilt resistance of plants or cultivating verticillium wilt resistant plant varieties and the like.

The invention relates to application of an OsMPK4 gene in enhancing low-temperature stress resistance in a rice seedling stage. Specifically, the rice OsMPK4 gene participating in positive regulation and control of low-temperature stress resistance is cloned in rice, so that the rice OsMPK4 gene is overexpressed, and compared with a wild plant, the rice OsMPK4 gene overexpressed plant shows the low-temperature-resistant characteristic, and the survival rate is remarkably increased. The discovery of the new function of the rice OsMPK4 gene provides a new gene target and resource for improving rice low-temperature stress resistance genetic breeding.

The invention discloses a lolium perenne seedling returning nitrogen reduction amount regulation and control method based on soil multifunctional evaluation, and relates to the technical field of agricultural planting.According to the method, a closed-loop dynamic regulation and control system is constructed, aiming at the soil characteristic difference after lolium perenne seedlings are returned to the field, precise and personalized management of the application amount of nitrogen fertilizer is achieved, and the application amount of nitrogen fertilizer is improved. According to the method, the nitrogen fertilizer compensation amount is dynamically adjusted according to the soil multifunctionality real-time evaluation result of the key growth stage of crops, and the yield fluctuation caused by blind reduction of application is effectively avoided, so that the stability and improvement of the crop yield are ensured while the chemical nitrogen fertilizer input is remarkably reduced, and the economic benefit is increased. The technical scheme that physical, chemical and biological indexes of soil are comprehensively evaluated in the key growth stage of crops, and the nitrogen fertilizer correction amount is dynamically calculated based on the deviation between the evaluation result and the target function range is adopted, so that the accurate regulation and control of the nitrogen fertilizer application amount can be realized; therefore, the stable yield of crops is guaranteed and the soil health level is continuously improved while the application of chemical nitrogen fertilizer is reduced.

The invention relates to the technical field of agricultural intelligent monitoring and regulation and control, and discloses a rice three-dimensional seedling raising seedling growth monitoring, diagnosis and regulation and control method, which comprises the following steps: S1, when any hanging basket reaches a target position, reducing the movement speed of the hanging basket, and obtaining a serial number image of the hanging basket; s2, when the hanging baskets stop moving, sequentially acquiring images right above and obliquely above all seedling trays in the hanging baskets corresponding to the serial number images of the hanging baskets; s3, respectively obtaining the areas of a green plant area, a yellow plant area, a black soil area and a seedling block area in the images right above and obliquely above the seedling tray, calculating the pixel number of each area, and obtaining the pixel ratio of different areas; and S4, acquiring the coverage rate, health index and spindling index of each seedling tray according to the ratio of pixels in different areas, dividing the seedlings in the seedling trays into five types of normal, spindling, vertical withering, insufficient fertilizer and strong, obtaining a growth vigor diagnosis result of the seedlings in the seedling trays, and generating a seedling growth vigor regulation and control prescription according to the growth vigor diagnosis result.

The invention relates to the technical field of agricultural equipment, and discloses a peanut film mulching hole-free seeder which comprises an integrated frame, and a connecting frame is arranged at the front end of the integrated frame and used for being connected with a traction machine in a hanging mode; according to the device, a weakening point is accurately preset on a mulching film through a liftable micro hot needle, the traditional manual or mechanical hole breaking is replaced, a hole-free seeding mode of one hole and one point is realized, and the hole-free seeding efficiency is greatly improved. According to the method, the complex procedure of manual film breaking is avoided, the labor intensity is reduced, through the controllable design of the weakening points, it is ensured that peanut seedlings naturally break the mulching film, damage to roots is reduced, the emergence rate and the survival rate are increased, and meanwhile the problem of weed breeding or soil moisture loss caused by too large broken holes is effectively solved.

The invention discloses a seed pelleting formula for improving salt tolerance of suaeda salsa in coastal saline-alkali soil and a processing method, the pelleting formula comprises 348-492 parts of a filler, 348-492 parts of a binder, 348-492 parts of a water-retaining agent and 348-492 parts of a biological stimulant, and the filler comprises attapulgite, talcum powder and vermiculite; the binder is 73-89 parts and comprises sodium carboxymethyl cellulose, polyvinyl alcohol and starch; the water-retaining agent accounts for 30 to 42 parts, and the water-retaining agent is polyacrylamide; the biological stimulant is prepared from the following components in parts by weight: 20-78 parts of yeast metabolite, potassium fulvate, seaweed essence, rhamnolipid and a microbial agent; the processing method comprises the steps of seed selection, raw material preparation, layered coating, low-temperature drying, selection and sieving, and polishing and shaping. According to the invention, a layered coating process is adopted to break through a traditional single mixed coating mode, an isolation breathable microenvironment is firstly constructed by the filling layer, then growth-promoting stress-resistant nutrient substances are injected by the active layer, and finally, the shaping layer is used for locking and shaping and strengthening protection, so that the seeding performance, salt tolerance, germination activity and seedling stress resistance of the pellets are remarkably improved.

The invention relates to the technical field of agricultural information and seed and seedling cultivation, and discloses a peanut planting method for relieving continuous cropping obstacles of peanuts. The method comprises the following steps: constructing initial digital twins of a land parcel, and synchronously constructing a disease-resistant peanut variety screening and seedling pretreatment system; historical continuous cropping information, soil physicochemical and microorganism data, meteorological time sequence data and seedling cultivation parameters are fused, the system is driven to evolve into operation state digital twins, and seedling-soil-environment collaborative simulation is achieved. Evaluating the continuous cropping obstacle risk and the seedling adaptability based on the twinborn body, and generating a comprehensive diagnosis report containing a nutrient imbalance index, pathogenic bacteria abundance prediction, a root exudates cumulative effect and a seedling disease-resistant adaptation coefficient; according to the method, differentiated seed and seedling optimization, soil improvement and cultivation strategies are implemented, and management measures are dynamically optimized through real-time data feedback in a growth cycle. Through cooperative driving of digital twinning and seed and seedling cultivation, source prevention, control and intervention of continuous cropping obstacles are achieved.

The invention particularly relates to a water-saving irrigation system for intelligent agricultural vegetable seedling raising, and relates to the technical field of intelligent agriculture. And a decision and processing module. According to the method, multi-dimensional data perception and intelligent decision are adopted, so that the core contradiction that disease prevention needs drying and seedling strengthening needs stress in traditional seedling culture is solved; through a non-contact infrared leaf temperature sensor, a miniature resistance-type leaf surface humidity-sensitive sensor and the like, key data such as leaf temperature and leaf surface humidity state are accurately collected, a leaf surface humidity prediction model is constructed, a disease risk window is divided in advance, and propagation of diseases such as downy mildew and gray mold is blocked from the source; the stress intensity is controlled through the wilting coefficient, so that the seedlings are prevented from withering due to excessive stress, and moderate drought is ensured to stimulate root growth.

The invention relates to the technical field of forestry intelligent monitoring, and particularly discloses an intelligent monitoring system for the growth condition of afforestation and greening nursery stocks based on deep learning, which is characterized in that multi-modal growth data of the nursery stocks are acquired through a multi-spectral imaging sensor, a three-dimensional laser scanning sensor and an environment monitoring sensor, and a standardized data set is formed through space-time alignment processing; extracting morphological structure and spectral response features through spatial domain and frequency domain parallel analysis, and generating multi-dimensional feature representation through cross-modal fusion; converting the parameters into growth state parameters by utilizing a feature recombination and space mapping technology; identifying an abnormal growth mode through time sequence dynamic analysis; and finally, adaptively adjusting the working parameters of the sensor according to the abnormal type to form closed-loop monitoring. The problem that an existing monitoring system cannot autonomously optimize a monitoring strategy according to the abnormal state is solved, and accurate monitoring and intelligent regulation and control of the nursery stock growth condition are achieved.

The invention discloses a corn seed environment adaptability cultivation method based on edge calculation, which comprises the following steps: step 1, acquiring environment factor data of a corn seed sowing area, and preprocessing the environment factor data; 2, extracting the edge contour of the corn seedling by adopting Canny edge detection and a Sobel operator based on the RGB image; 3, constructing a multi-source heterogeneous data sample set according to time and space coordinate alignment; 4, constructing a causal structure diagram by adopting an improved NOTEARS algorithm; 5, carrying out comprehensive sorting to obtain a key factor path; step 6, inputting the multi-dimensional environment factor vector of the target area to the starting node of the key factor path to obtain a predicted phenotypic feature vector; and 7, comparing the actual phenotypic feature vector with the predicted phenotypic feature vector, and counting a prediction error. According to the method, causal modeling and image analysis are fused, and the breeding efficiency and the accuracy of regional suitability judgment are improved.

The invention provides an automatic illumination adjusting method and system based on seed and seedling cultivation, and relates to the technical field of illumination control and intelligent agriculture, the method comprises the following steps: firstly, collecting seedling chlorophyll fluorescence parameters to construct a light stress index, and accurately identifying a light inhibition risk; then calculating a physiologically permissible light intensity threshold adaptive to the dark response capability of the plant by combining the environment temperature and the CO2 concentration; positioning a shielded weak light area by using three-dimensional point cloud voxelization and a ray tracing technology, and calculating a shadow compensation gain coefficient; and finally, generating a control matrix in combination with a dynamic left-rotation light strategy, and driving an independent addressing LED array and a variable-angle lens to execute local precise light supplement and light vector adjustment. Through physiological feedback and spatial light field reconstruction, the problem that traditional illumination control neglects individual tolerance difference and canopy shielding is solved, photooxidation damage is effectively prevented, and the growth uniformity and the light energy utilization rate of seedling groups are remarkably improved.

The invention discloses beneficial fusarium oxysporum and application thereof in relieving apple continuous cropping obstacles, and belongs to the technical field of agricultural microorganisms. The biological preservation number of the fusarium oxysporum FoB1 disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) NO.42195. The fusarium oxysporum FoB1 disclosed by the invention is beneficial fusarium oxysporum, and has the advantages of excellent biocontrol function, strong environmental adaptability and high affinity with a host. By optimizing the preparation process of the microbial inoculant, the activity and stability of the inoculant are remarkably improved, and a high-quality bacterium source is provided for large-scale production. On the basis, a simple and convenient bacterium-bearing nursery stock application technology is developed, a novel efficient biological prevention and control way is provided for relieving apple continuous cropping obstacles, and important technical support is provided for promoting green and sustainable development of the fruit tree industry.

The invention discloses an ecological value calculation method based on scene application and ecological behaviors. The method comprises the following steps: acquiring multi-source data required by ecological value calculation; cleaning and standardizing the multi-source data, removing abnormal values, complementing missing data, and generating a structured data set which can be used for modeling; an ecological value calculation model is constructed, and the model comprises a scene application ecological value model used for calculating ecological service values in different marketization scenes; the ecological behavior value contribution model is used for calculating ecological value contributions of ecological behaviors of seedling planting, soil restoration and water source restoration developed by the social subject; and the ecological value calculation model generates an ecological value calculation report based on the structured data set, wherein the report comprises each index weight, a calculation process and an ecological value result. According to the method, ecological behavior differences and regional characteristics are considered, and scientificity, applicability and economic transformation benefits of an ecological value calculation result are improved.

The invention provides a sunlight greenhouse winter seedling raising method for angelica sinensis. The Chinese angelica sunlight greenhouse winter seedling raising method comprises the steps that a sunlight greenhouse is built in an area with the altitude being 2000 m or below, the azimuth angle of the sunlight greenhouse is 5-7 degrees from the south to the west, the base angle of a front roof is 60-65 degrees, the elevation angle of a main lighting surface is 22-25 degrees, and the elevation angle of a back slope is 37-38 degrees; the method comprises the following steps: sowing angelica sinensis seeds in a greenhouse from late November to early and middle December, wherein the seeds comprise early bolting seeds, side ear seeds or main ear seeds; the environment in the greenhouse is controlled, the temperature in the coldest month is not lower than 12 DEG C, the ground temperature is not lower than 10 DEG C, and vernalization of the angelica sinensis seedlings is avoided; managing the seedlings, including water management, sunlight management, temperature regulation and control, weeding and thinning, and disease and pest control. The Chinese angelica sunlight greenhouse winter seedling raising method provided by the invention has the advantages that Chinese angelica seedling raising is carried out in a zone with the altitude of 2000m or below, and the problem that the ecological environment is damaged by Chinese angelica early bolting, Chinese angelica seed reserving, Chinese angelica prepared land seedling raising and Chinese angelica high-altitude seedling raising can be solved.

The invention discloses a multi-row planting rice transplanter, and belongs to the technical field of agricultural power machinery, the multi-row planting rice transplanter comprises a seedling hopper, a frame body of the seedling hopper is provided with a plurality of parallel inclined bottom grooves, seedlings are vertically stacked in the inclined bottom grooves, and the downstream of each inclined bottom groove is provided with a seedling discharge port for the seedlings to be smoothly taken out; a main adjusting frame of the base is fixed below the seedling hopper. By arranging the contactors with the variable gaps and the matched adjusting assemblies of the contactors, lossless seedling taking and reliable clamping of seedlings are achieved. When the contactor is close to the seedling discharging opening from bottom to top, the variable gap is automatically enlarged by the adjusting assembly, so that a plurality of seedlings smoothly enter the fork head, and the damage of stems and leaves caused by too narrow opening is avoided; when the contactor moves downwards to the seedling discharging opening, the gap is rapidly reduced, seedling stems are actively clamped, friction force between seedlings is overcome, and it is ensured that the seedlings synchronously move downwards along with the contactor. The dynamic regulation mechanism remarkably reduces the seedling taking damage rate and improves the seedling integrity.

The invention discloses a seed coating containing an arbuscular mycorrhizal inoculant. The seed coating is prepared from the following components in parts by weight: 25 to 35 parts of the arbuscular mycorrhizal inoculant, 35 to 45 parts of a soil conditioner, 15 to 20 parts of talcum powder and 15 to 20 parts of polyvinyl acetate. The invention further provides a preparation method of the seed coating containing the arbuscular mycorrhizal inoculant and application of the seed coating to reduction of soil heavy metal pollution, increase of disease resistance and stress resistance of crops and promotion of crop growth. The seed coating containing the arbuscular mycorrhizal inoculant wraps the surfaces of plant seeds, provides good nutritional ingredients for growth and development of the seeds, and is beneficial to improving the germination rate of the seeds under heavy metal cadmium stress, reducing soil heavy metal cadmium pollution, improving soil quality, increasing soil fertility and improving disease resistance and stress resistance of crops; growth of seedlings is promoted, and crop quality and yield are improved.

The invention discloses a rice seedling leaf age intelligent identification system based on a depth camera, and belongs to the technical field of agricultural intelligent equipment and machine vision. Comprising a monitoring point gridding path planning module, a mechanical arm cooperative positioning data acquisition module, an RGB-D data preprocessing module, a dynamic foreground seedling accurate extraction module, a leaf age recognition confidence evaluation module, a leaf age data visualization presentation module and an integrated control strategy feedback module. RGB color features and connected domain analysis are combined to optimize a mask, a front single seedling is accurately segmented, background interference is eliminated, the confidence coefficient is dynamically calibrated through a deep learning model and image quality features, texture definition and edge continuity features are combined to generate calibrated confidence coefficient, confidence coefficient evaluation is directly associated with an agricultural decision, and the accuracy of the agricultural decision is improved. The leaf age result is generated only based on reliable data, the personnel rechecking demand is reduced, and the decision-making efficiency is improved.

The invention discloses an aquatic seedling counting and growth parameter measuring system, which comprises the following steps: acquiring a seedling flow image sequence and generating an image confidence score, selecting kernel function width according to different confidence conditions to construct a density map, and obtaining a density distribution feature vector capable of representing a spatial aggregation state; extracting morphological features of the seedlings under a multi-scale receptive field based on the feature vectors, constructing a multi-scale feature pyramid, calculating a spatial overlapping degree index according to a difference relationship between scales, and identifying a dense region; the fusion weight of the multi-scale features is determined in combination with an overlapping degree index, and fusion features are generated and used for executing counting and growth parameter measurement; and constructing a performance deviation index according to the counting accuracy and the measurement precision, and performing joint updating on the kernel function parameters and the fusion weight when the deviation exceeds a threshold value to form an optimized path of which the parameters can be iteratively updated so as to realize stable counting and accurate measurement under complex density change and overlapping conditions.

The invention provides a molecular marker composition related to rice seedling stage cold resistance, a primer composition, a kit and application thereof, and belongs to the technical field of crop genetic breeding. The invention provides a molecular marker composition related to rice seedling stage cold tolerance. The molecular marker composition comprises at least one of a first SNP marker, a second SNP marker, a third SNP marker, a fourth SNP marker, a fifth SNP marker and an InDel marker. The invention further provides a primer composition for detecting the cold resistance of the rice in the seedling stage. The primer composition comprises at least one of a first primer group, a second primer group, a third primer group, a fourth primer group, a fifth primer group and an InDel detection primer group. The molecular marker composition and the primer composition disclosed by the invention can be used for accurately distinguishing a cold-tolerant rice variety and a cold-sensitive rice variety in a seedling stage, and scientific guidance is provided for breeding and improvement of a new cold-tolerant rice variety.

The invention discloses a Chinese wolfberry seedling raising method for improving paddy field soil, which comprises the following specific steps: collecting cutting slips: collecting branches with the diameter of more than 0.3 cm, cutting the branches into cutting slips with the length of 12-15 cm, and reserving 2-3 tender shoots on each cutting slip without damage; rooting liquid treatment: preparing a rooting liquid, and soaking the positions 3-5 cm above the bottom ends of the cutting slips in the rooting liquid for 12-24 hours; the rooting liquid is mainly prepared from rice vinegar and brown sugar; temperature-controlled root promoting: controlling the environment temperature to be 15-25 DEG C, and vertically inserting the cutting slips treated by the rooting liquid into the seedling raising container; the seedling raising container is filled with improved paddy field soil in advance; when 1-2 tender shoots newly grow on the cutting slips and 7-8 tender leaves grow on the cutting slips, shoot tips are removed, so that the tender shoots on the cutting slips are converted into semi-lignified from fleshy, apical dominance is inhibited, rooting is promoted, and seedling raising is continued; when new buds grow out again, seedling raising is completed, and the seedlings are transplanted to a field for planting. The lycium barbarum seedling raising method has the advantages of being low in cost, environmentally friendly, high in survival rate, suitable for improved paddy field soil and the like.