53 results about "Paris polyphylla var. yunnanensis" patented technology

The invention relates to a Paris polyphylla var. yunnanensis scale seedling culturing method. The method comprises the following steps of: selecting Paris polyphylla var. yunnanensis plants more than 10 years old and reserving seeds for planting; collecting mature seeds; washing the collected seeds to remove episperm; spreading and drying the seeds in a shade and ventilating place within 12 hours, wherein a temperature is maintained and an accumulation thickness is less than or equal to 6 cm; spreading the seeds uniformly and drying; filling the seeds into a ventilating container; storing the container in a shade environment with a suitable temperature, wherein the storage time is no more than 2 months; clearing impurities and residues in a seedling culturing field; fertilizing decayed farm manure and calcium superphosphate; plowing and raking soil; forming a furrow; spreading humus soil on the furrow surface; flatting the furrow surface; sowing in early June next year; taking the stored seeds, and after sterilizing, broadcasting the seeds uniformly according to the thickness of sowing; earthing after broadcasting; covering mantle on the furrow surface; irrigating sufficiently and performing seedling management. A method for drying the seeds of the Paris polyphylla var. yunnanensis in shade in an environment without direct sunlight is adopted, the dormancy of the seeds of the Paris polyphylla var. yunnanensis is broken, and the scale production of Paris polyphylla var. yunnanensis seedlings is realized, so that germchit guarantee is provided for large-area popularization and cultivation of the Paris polyphylla var. yunnanensis. The method has the characteristics of simple and easily-learned operation, high survival rate of germchit, low production cost, healthy and strong germchit, stable and reliable technology, high repeatability, and capacity of effectively solving the problem of scale seedling cultivation of the Paris polyphylla var. yunnanensis.

The invention provides a two-stage cultivation method for Paris polyphylla var. yunnanensis(Franch.)hand.-mazz.. The method comprises three parts, i.e. high-quality seedling raising, commercial Paris polyphylla var. yunnanensis(Franch.)hand.-mazz. cultivation and field management, wherein the high-quality seedling raising step comprises seed germination and seed seedling raising, seeds are timely applied with a leaf fertilizer and topdressed with farm manure after germinating, water management is reinforced, and seedlings are transplanted after being cultivated for 3 to 4 years; the commercial Paris polyphylla var. yunnanensis(Franch.)hand.-mazz. cultivation step comprises land selection and preparation, the three to four-year-old seed seedlings are transplanted according to seedling size grades after being tumbled in November to December, the number of large seedlings (equal to or heavier than 10 grams) is 20,000 to 250,000, and the number of small seedlings (5 to 10 grams) is 250,000 to 30,000; after the transplanted seedlings survive, the leaf fertilizer is topdressed in a vigorous growth period, farm manure is timely topdressed after sprout tumble in winter, and a Paris polyphylla var. yunnanensis(Franch.)hand.-mazz. medicinal material can be harvested after 4 to 5 years. According to the method, the investment cost is effectively shared, the investment cycle is reasonably divided, Paris polyphylla var. yunnanensis(Franch.)hand.-mazz. seedling propagation and commercial medicinal material cultivation are normalized and specialized, and the healthy and rapid development of the industry is promoted.

The invention discloses a seed seedling raising method for Paris polyphylla var. yunnanensis(Fr.)hand.-mazz.. By the method, the rate of emergence is increased, and the seedling rate is increased. Paris polyphylla var. yunnanensis(Fr.)hand.-mazz. seeds subjected to stratification treatment and post ripening are uniformly broadcast on a prepared artificial substrate according to 600/800 per square meter, and by the loose, air-permeable and water-retention substrate, the good development of root systems in an early Paris polyphylla var. yunnanensis(Fr.)hand.-mazz. seed germination process is ensured, so that the rate of emergence is increased, and can reach more than 80 percent; by leaf dressing, the nutrition of seedlings is added, the growth of tubers of the Paris polyphylla var. yunnanensis(Fr.)hand.-mazz. seed seedlings is promoted, the stress resistance of the seedlings is improved, the seedling rate is greatly increased, and reaches more than 60 percent, the utilization rate of Paris polyphylla var. yunnanensis(Fr.)hand.-mazz. seed resources is increased, the good development of Paris polyphylla var. yunnanensis(Fr.)hand.-mazz. plantation is promoted, and special rare medicinal material resources in China are effectively protected.

The invention relates to the technical field of agriculture and forestry seedling cultivation, in particular to a quick cultivation method of Paris polyphylla seedlings. The quick cultivation method includes the steps of seed selection, seed coat softening, process steps like ultrasonic treatment, low-temperature seedling accelerating, normal-temperature sprouting and seedling cultivation. By the quick cultivation method, cultivation cycle of the Paris polyphylla seedlings is shortened greatly to 100-120 days from 600 and more days needed in a conventional seedling cultivation method, and Paris polyphylla seedlings cultivation cost is lowered from 0.8RMB/plant to 0.3RMB/plant, so that cultivation cost of the Paris polyphylla seedlings is lowered.

The invention relates to a tuber breeding and culturing method of paris polyphylla, and belongs to the technical field of agriculture and forestry seedling raising techniques. According to the method, healthy and mature paris polyphylla is obtained in January of the last year, cut into irregular blocks as small as broad beans, soaked in liquid medicine for 20-30 minutes, dried, put into a thermotank for wound processing, then soaked in a rooting powder solution of 15 percent for 15 minutes, taken out, dried, put into a frozen tank for dormancy processing and then subjected to a dormancy breaking processing for three days in a 22DEG C thermotank after 20 days of dormancy. The above process is repeated three times, after the third time of process, tubers are taken out and put in warm sand of the 70 percent humidity for accelerating germination, after about 35 days, buds are grown out, and the tubers are planted in April. The tuber breeding and culturing method of paris polyphylla effectively improves the breeding speed of the paris polyphylla, largely reduces the seedling culturing cost, controls the excavation of wild paris polyphylla, and protects the natural ecological resources to some extent.

The invention relates to an alternate cooling and heating paris polyphylla seed propagation method, which comprises the three major steps including seedbed selection and processing, seed selection and seed processing, and seeding, wherein a concrete seed selection and seed processing method comprises the steps that full and ripe paris polyphylla seeds with the thousand seed weight being 40 to 70g and without disease, mildew or damage are selected, and the seeds are soaked for 10 minutes by 30PPM GA for 10 minutes before the germination acceleration; then, the seeds are mixed with clean fine sand according to a mixture ratio of 2:1, episperm is removed through rubbing, and the seeds are cleanly cleaned and are soaked for 1 hours by 500 times of carbendazim; the processed seeds are buried into the wet sand to be processed for 2 months at 5 to 10 DEG C, are then placed to an 18-20-DEG C environment to be processed for 3 months, are next placed into a 5-10-DEG C environment to be processed for 1.5 months, and are then placed into a 20-22-DEG C environment to be processed for 1.5 months, and the attention is paid on the moisture preservation in the period. When the method provided by the invention is adopted, the germination period of the paris polyphylla seeds can be reduced from two years into one year, in addition, the germination rate of the paris polyphylla seeds is effectively improved, and the germination rate reaches more than 90 percent.

The invention discloses a large-scale cultivation method for paris polyphylla seedlings. The method includes the following steps that firstly, selected land is subjected to deep tillage and sun exposure, then base fertilizer is applied, furrow opening is conducted, and humus soil is scattered on the furrow face; afterwards, harvested fruits are peeled and soaked by warm water and gibberellin, and the soaked fruits are evenly mixed with sand for dark light storage; sowing can be performed after storage for 2-5 months, the temperature and humidity of the interior of an arched shed need to be reasonably controlled after sowing is completed, and reasonable topdressing is carried out. By means of the large-scale cultivation method, the emergence rate of the seedlings is effectively increased and can reach 85%; moreover, emergent seedlings are neat, the growth vigor of the seedlings is high, rooting is fast, and the survival rate of the seedlings can reach 96%. The method has the advantages of being easy to operate, low in cost, easy to popularize, and capable of effectively shortening the germination period of paris polyphylla seeds and simplifying the seedling raising technique of the paris polyphylla seeds. Benign development of the paris polyphylla planting industry is effectively promoted, and good economic benefits and social benefits are achieved.

The invention provides a preparation method of polyphyllin II and polyphyllin VII, and in particular provides a method for preparing the polyphyllin II and the polyphyllin VII from peel of paris polyphylla var. yunnanensis. The method comprises the steps of crushing the peel of the paris polyphylla var. Yunnanensis, extracting by a solvent to obtain a crude extract, enriching the polyphyllin II and the polyphyllin VII from the crude extract so as to obtain parts which are rich in the polyphyllin II and the polyphyllin VII, performing further separation and purification to obtain the polyphyllin II and the polyphyllin VII. Through the method provided by the invention, the purpose of preparing the polyphyllin II and the polyphyllin VII in large scale can be realized.

The invention provides a polygerm paris yunnanensis franch. underground rhizome cutting propagation method. According to the polygerm paris yunnanensis franch. underground rhizome cutting propagation method, combination of rhizome cutting propagation technology with dominant varieties is realized; full utilization of polygerm paris yunnanensis franch. polygerm germination characteristic is realized; and oblique cutting method and accelerating germination method are adopted so as to promote germinating of paris yunnanensis franch. underground rhizome surface resting buds and obtain new plants. According to the polygerm paris yunnanensis franch. underground rhizome cutting propagation method; a special soil formula is adopted, appropriate temperature, humidity, and gas permeability are maintained; no decay is caused in germination accelerating process; and rapid industrialization of paris yunnanensis franch. is realized. The polygerm paris yunnanensis franch. underground rhizome cutting propagation method is used for producing cutting seedlings, seedling development rate is as high as 95%, cutting loss is less than 5%, polygerm rate is higher than 90%; expanding propagation of 666.7m<2> of paris yunnanensis franch. into more than 6667m<2> is realized; germination accelerating time is 1 year or less; seedling supplying can be realized in one year, and is 2 to 3 years earlier than that of seed seedling. The obtained seedlings are strong, developed in root system; flowering and seeding in the same year can be realized; seeds can be produced; and the medicinal materials can be supplied in 5 years of plantation.

The invention discloses a culture method for paris polyphylla var. yunnanensis seeds. The method comprises the steps of seed selection, seed washing and peeling, first-time low-temperature storage, peeling culture, second-time low-temperature storage and seed germination culture. According to the culture method, through simulating a winter low-temperature environment in the natural world and carrying out dormancy breaking twice by adopting low-temperature treatment twice, the dormancy stage of the paris polyphylla var. yunnanensis seeds can be effectively broken, the germination period of paris polyphylla var. yunnanensis is shortened to 90 to 125 days from two years, the germination rate is increased to 85% to 95% from 30% to 50%, the germination period of the paris polyphylla var. yunnanensis seeds is shortened, the germination of the paris polyphylla var. yunnanensis seeds is accelerated, the germinating rate of the paris polyphylla var. yunnanensis seeds is increased, and the problems that the paris polyphylla var. yunnanensis is long in nursery stage and low in germination rate are solved; and the culture method is simple and easy and is low in cost, and a technical support is provided for the propagation and large-scale planting of the paris polyphylla var. yunnanensis.

The invention discloses a method for rapidly breeding paris polyphylla, mainly aiming at solving the problem of the existing paris polyphylla tuber breeding way in the prior art that, only one bud can be bred by using one tuber, a smaller root is formed, and growing is slow, so that the demand of the existing market on polyphylla breeding cannot be met. The perennial and robust paris polyphylla rhizomes which are not damaged and do not have plant diseases and insect pests are selected as a provenance for breeding; the method comprises the steps of digging out the fresh paris polyphylla rhizomes, washing and then drying in the air, and cutting off adventitious roots on the rhizomes; after that, washing stem internodes with sterile water, then cutting off buds on the stem internodes, and cutting the stem internodes of the rhizomes into blocky structures; inoculating the blocky structures into a breeding culture medium for culturing; when a first true leaf and five adventitious roots grow on one blocky structure, transplanting the blocky structure into soft soil for growing. After the scheme is adopted, the aims of rapidly breeding the paris polyphylla and enabling the paris polyphylla to take more roots are achieved.

The invention relates to a Paris polyphylla var. yunnanensis endogenetic Gliomastix used to produce volatile oil. The endogenetic fungi Ppf8 is acquired from Paris polyphylla var. yunnanensis by the endogenetic fungi isolation technique, and is classified and determined to be Gliomastix. The bacterial strain is preserved in China General Microbiological Culture Collection Center with the registered number of CGMCC No. 2474. Endogenetic Gliomastix used to produce volatile oil is isolated from Paris polyphylla var. yunnanensis for the first time herein, and the chemical compositions and the antibacterial activity of the volatile oil are identified. The invention is to make use of the endogenetic fungi resource of plant to acquire natural active ingredients.

The invention discloses a penicillium brefeldianum strain and application thereof. The penicillium brefeldianum strain is PFLJ04, the preservation name of the strain is penicillium brefeldianum, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC), the address of the preservation unit is No.3, Yard 1, West Beichen Road, Chaoyang District, Beijing, and the preservation date is August 15, 2019; and the preservation number is CGMCC No. 18119. Penicillium is separated from paris polyphylla var. yunnanensis endophyte, and has a good inhibiting effect on panax notoginseng root rot fungi (fusarium oxysporum, fusarium solani and phytophthoracactorum), paris polyphylla leaf spot fungi and paris polyphylla botrytis cinerea (botrytis cinerea, phoma sp. and pestalotiopsis sp.) simultaneously.

The invention discloses a method for promoting the growth of paris polyphylla seedlings through paris polyphylla endophytic bacteria. The method comprises the steps of extraction of endophytic bacteria, potting soil treatment, seedling transplanting, field management and root-irrigation inoculation. According to the method, on the basis of endophytic bacteria resources, the growth-promoting effectis brought to the paris polyphylla seedlings, endophytic bacteria inoculation is conducted on the paris polyphylla seedlings, increasing of plant height and plant weight of the paris polyphylla seedlings can be effectively promoted, the chlorophyll content of the paris polyphylla seedlings is increased, and meanwhile the application dose of chemical fertilizers and chemical pesticides in the cultivation process of paris polyphylla is reduced.

The invention discloses a method increasing Paris polyphylla var yunnanensis maturing rate. The method comprises the following steps of (1) selecting strong and free of insect diseases paris polyphylla var yunnanensis plants, collecting pollen from most plants with cracked pollen sacs after the plants blossoms, (2) utilizing a portable dust collector to collect thoroughly-grown and rich pollen groups to a polypropylene filtration film with the diameter of 10mm and an aperture of 450nm, (3) conducting hand-pollinating three days after most pollen sacs crack, dipping the collected pollen via a writing brush or a soft brush and then evenly coating the pollen on the Paris polyphylla var yunnanensis necking head. Each necking head is dipped for two times; hand-pollination can be conducted from 4pm to 8pm and finished on the day; the next day, the same operations are conducted; and hand-pollination is conducted once more. Paris polyphylla var yunnanensis maturing rate can be improved by more than 40%, so seed source is enlarged for agricultural production and higher promotion value can be achieved.

The invention belongs to a cultivation technique for plantation of plants, and specifically relates to a floating seedling-raising method for amyloid Paris polyphylla var. yunnanensis rhizomes. The floating seedling-raising method for the amyloid Paris polyphylla var. yunnanensis rhizomes has a concrete technical scheme as follows: A, addition of a nutrient solution into a nutrient pool; B, filling with a substrate; C, burying with the amyloid Paris polyphylla var. yunnanensis rhizomes; and D, management. The floating seedling-raising method for the amyloid Paris polyphylla var. yunnanensis rhizomes has a short seedling-raising period, shortens rejuvenation time, saves labor, avoids soil-borne diseases and pests and environment-caused diseases, and has high survival rate.

The invention relates to a cultivation method of Paris polyphylla and belongs to the technical field of agricultural planting. The cultivation method includes: at a seedling growing stage which is November of each year, selecting plateau sand land, preparing soil, fertilizing, sowing seeds of Paris polyphylla, sequentially covering humus fine powder and pine leaves, regularly watering and weeding, sheltering from sunlight in the next year, cultivating outdoors in the third year, and completing seedling growing in the fourth year; at a cultivation stage which is June afterwards of the fourth year of seedling growing, starting transplanting, still selecting plateau sand land, fertilizing, leveling, covering humus after transplanting is completed, regularly watering and weeding, paving a layer of humus and pine leaves in winter respectively, and cutting sprouts to obtain finished products starting from the fifth year of seedling growing, wherein less than 10000 plants are transplanted for each mu, and seedling planting depth is 5-7 cm from the ground. Weight of dry Shen Paris polyphylla after being transplanted by five years reaches higher than 1kg, and saponin content can reach 2.2-2.8.

The invention relates to a Paris polyphylla var. yunnanensis endogenetic Pichia pastoris used to produce volatile oil. The endogenetic fungi Ppf9 is acquired from Paris polyphylla var. yunnanensis by the endogenetic fungi isolation technique, and is classified and determined to be Pichia. The bacterial strain is preserved in China General Microbiological Culture Collection Center with the registered number of CGMCC No. 2475. Endogenetic Gliomastix used to produce volatile oil is isolated from Paris polyphylla var. yunnanensis for the first time herein, and the chemical compositions and the antibacterial activity of the volatile oil are identified. The invention is to make use of the endogenetic fungi resource of plant to acquire natural active ingredients.

The invention discloses a hole-ground film direct sowing culture method for paris polyphylla. The method comprises the following steps: selecting seeds which are full in buds and are fully ripened, peeling, sterilizing, directly seeding the seeds on a black film which is pretreated and sterilized and is provided with direct sowing holes, covering the film on a nursery garden, retaining that the moisture content of soil is between 50 and 60 percent after seeding, completely weeding, and preventing and controlling plant diseases and insect pests of paris polyphylla after seedling emergence by adopting a physical trapping method. Compared with the traditional culture method, the method disclosed by the invention has the advantages that the seedling emergence rate of the seeds is effectively improved, the manual cost generated during seed sprouting and transplanting is saved, the resources are greatly saved, any chemical pesticide residue is avoided, the quality and drug effect of paris polyphylla can be completely remained, and the planting method is simple in technical program and easy to perform, is a direct seed sowing technology with the advantages of simplicity, effectiveness, low cost, high germination rate, good growth vigor and high disease-resistant immunity, is prominent in effect and is easy for large-area cultivation promotion.

The invention relates to a planting method, in particular to imitating the wild planting of Prunus chinensis under the chestnut tree. Solve the problem of the wild planting of double buildings under the chestnut tree. The following steps are taken to imitate the wild planting of chrysanthemum under the chestnut tree: ① soil moisture adjustment: deep plow the soil under the forest where the chrysanthemum grows by 30-45 cm, and then shallow plow and fine rake to level the moisture before planting; ② build shade Shed: Combined with the planting conditions of the chestnut tree, cover with a sunshade net with a shading degree of 60-70%; ③Transplanting: In the first ten days of October, the seedlings of Dianzhonglou were placed in the shade shed with a row spacing of 15×15cm. After cultivation and transplanting, spread a layer of sun-dried Chestnut branches and leaves; ④Conventional management, after 3 to 5 years of cultivation, after the plants wither from mid-October to early November, excavate the rhizomes of Dianzhonglou to obtain mature The Chinese herbal medicine Dian Chonglou. It has the advantages of simple operation, easy popularization, high income and low risk.

The invention discloses a culture medium capable of increasing the emergence rate of paris polyphylla and a seedling raising method of the medium. The culture medium comprises a seedling raising medium and a sowing medium, wherein the seedling raising medium is formed by mixing river sand, vermiculite, a compound fertilizer and fresh cow dung according to the mass ratio of 3: 2: 1: 1.5, and the compound fertilizer comprises the following effective components in percentage by mass: 15% of N, 15% of P2O5 and 15% of K2O; and the sowing medium is formed by mixing peat soil, vermiculite, a compound fertilizer and decomposed chicken manure according to the mass ratio of 3: 1: 1.5: 1, and the compound fertilizer comprises the following effective components in percentage by mass: 15% of N, 15% of P2O5 and 15% of K2O. The culture medium can increase the emergence rate and the seedling rate of the paris polyphylla remarkably. The seedling raising medium and the sowing medium are good in air permeability and water retention performance, the requirement for root development in the paris polyphylla seed germination process is met, the formula of fresh cow dung and the like is added into the seedling medium, and it can be effectively guaranteed that the emergence rate is increased and reaches 83% or above.

The invention provides a method for accelerating biomass accumulation of paris polyphylla by increasing the carbon dioxide concentration. The method comprises the steps of increasing the carbon dioxide concentration in the environment, accumulating the biomass of paris polyphylla, pre-culturing paris polyphylla, and achieving carbon dioxide concentration treatment and culture. The invention relates to the technical field of bioengineering. The method is a novel means for promoting quick biomass accumulation of paris polyphylla by increasing the carbon dioxide (CO2) concentration. Increase of the carbon dioxide concentration in the environment accelerates accumulation of fresh weight of paris polyphylla seedlings. The method has a production promotion function in actual production. By changing and increasing the content of main carbon sources required by the paris polyphylla in the environment, the requirement of actual production for yield increase of the paris polyphylla is met, and the cultivation and planting efficiency of the paris polyphylla is further improved.

The invention provides a cultivation method of paris polyphylla. The cultivation method is characterized by comprising the following steps: planting field selection; shading shed construction; land consolidation; seed propagation; transplantation and field planting; field management; pest control and prevention; and harvesting. The cultivation method of paris polyphylla, provided by the invention,is standard and simple; due to process control, the yield of the paris polyphylla can be effectively increased; due to the comprehensive use of a great number of farm manure and microelement fertilizers, the condition of an organic/inorganic compound fertilizer is simulated, and the demand for fertility in a paris polyphylla production process is increased; and meanwhile, a brand-new seed germination way is adopted in the cultivation method, so that the problem that paris polyphylla seeds are after-matured is effectively solved, and the germination rate of the seeds is high.