Method for promoting growth of paris polyphylla seedlings through paris polyphylla endophytic bacteria

A technology of endophytic bacteria and Parisia diarrhea, applied in horticultural methods, botanical equipment and methods, planting substrates, etc., can solve the problems of planting and growth inhibition of Parisia diarrhea, affecting the quality of Parisia diangi, and destroying the ecological environment, etc. Achieve the effect of improving the overall economic and ecological benefits, promoting photosynthesis, and reducing the damage to the ecological environment

Active Publication Date: 2019-12-03
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In addition, farmers use a large amount of chemical fertilizers and chemical pesticides in the planting process of Dianzhong...

Method used

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  • Method for promoting growth of paris polyphylla seedlings through paris polyphylla endophytic bacteria
  • Method for promoting growth of paris polyphylla seedlings through paris polyphylla endophytic bacteria
  • Method for promoting growth of paris polyphylla seedlings through paris polyphylla endophytic bacteria

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Experimental program
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Effect test

Embodiment 1

[0034] Step S1: Endophytic Bacteria Extraction

[0035] Step S1.1: Rinse the harvested Parisia diatriana with tap water, select healthy rhizome tissue of Parisia diangi and cut it into small sections, and dry the surface moisture with filter paper;

[0036] Step S1.2: Disinfect the surface of the tissue of Parisia diatriana, soak it in ethanol with a mass concentration of 75% for 3 minutes, wash it with sterile water for 3 times, then soak it with a sodium hypochlorite solution with a mass concentration of 10.9% for 3 minutes, and finally wash it with sterile water 3 times;

[0037] Step S1.3: Grind the surface-sterilized Pachyphysis Dianchi tissue in a sterilized mortar, add sterile water to stir and mix well, then transfer to LB liquid medium, set the shaker temperature to 37°C, Shake at 180 rpm for 3 hours to make a bacterial suspension;

[0038] Step S1.4: Spread the bacterial suspension on the LB plate medium, and place it in a 37°C incubator for 24 hours;

[0039] Ste...

Embodiment 2

[0049] Step S1: Endophytic Bacteria Extraction

[0050] Step S1.1: Rinse the harvested Parisia diatriana with tap water, select healthy rhizome tissue of Parisia diangi and cut it into small sections, and dry the surface moisture with filter paper;

[0051] Step S1.2: Disinfect the surface of the tissue of Parisia diatriana, soak it in ethanol with a mass concentration of 75% for 3 minutes, wash it with sterile water for 3 times, then soak it with a sodium hypochlorite solution with a mass concentration of 10.9% for 3 minutes, and finally wash it with sterile water 3 times;

[0052] Step S1.3: Grind the surface-sterilized Pachyphysis Dianchi tissue in a sterilized mortar, add sterile water to stir and mix well, then transfer to LB liquid medium, set the shaker temperature to 37°C, Shake at 190rpm for 2.5h to make a bacterial suspension;

[0053] Step S1.4: Spread the bacterial suspension on the LB plate medium, and place it in a 37°C incubator for 36 hours;

[0054] Step S1...

Embodiment 3

[0064] Step S1: Endophytic Bacteria Extraction

[0065] Step S1.1: Rinse the harvested Parisia diatriana with tap water, select healthy rhizome tissue of Parisia diangi and cut it into small sections, and dry the surface moisture with filter paper;

[0066] Step S1.2: Disinfect the surface of the tissue of Parisia diatriana, soak it in ethanol with a mass concentration of 75% for 3 minutes, wash it with sterile water for 4 times, then soak it with a sodium hypochlorite solution with a mass concentration of 10.9% for 3 minutes, and finally wash it with sterile water 4 times;

[0067] Step S1.3: Grind the surface-sterilized Pachyphysis Dianchi tissue in a sterilized mortar, add sterile water to stir and mix well, then transfer to LB liquid medium, set the shaker temperature to 37°C, Shake at 200 rpm for 2 hours to make a bacterial suspension;

[0068] Step S1.4: spread the bacterial suspension on the LB plate medium, and place it in a 37°C incubator for 48 hours;

[0069] Ste...

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Abstract

The invention discloses a method for promoting the growth of paris polyphylla seedlings through paris polyphylla endophytic bacteria. The method comprises the steps of extraction of endophytic bacteria, potting soil treatment, seedling transplanting, field management and root-irrigation inoculation. According to the method, on the basis of endophytic bacteria resources, the growth-promoting effectis brought to the paris polyphylla seedlings, endophytic bacteria inoculation is conducted on the paris polyphylla seedlings, increasing of plant height and plant weight of the paris polyphylla seedlings can be effectively promoted, the chlorophyll content of the paris polyphylla seedlings is increased, and meanwhile the application dose of chemical fertilizers and chemical pesticides in the cultivation process of paris polyphylla is reduced.

Description

technical field [0001] The invention belongs to the technical field of Chinese herbal medicine cultivation, and in particular relates to a method for promoting the growth of Chonglou diangi seedlings by endophytic bacteria. Background technique [0002] Dianzhonglou is a perennial herb belonging to the genus Paris L. of Liliaceae, mainly distributed in Yunnan, Guizhou and Sichuan. Modern pharmacological research shows that it is mainly used to treat snake bites and bruises. Its rhizomes have the functions of reducing swelling and blood stasis, cooling liver and calming convulsions, clearing away heat and detoxification, and are national essential medicines. They are clinically used for hemostasis and anti-tumor , Tonsillitis, laryngitis, mumps and upper respiratory tract infection embolism. Dianzhonglou is the main ingredient of famous Chinese patent medicines such as "Yunnan Baiyao", "Jidesheng Snake Tablet", "Reduqing" and "Gongxuening". It has a long history of use and i...

Claims

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Application Information

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IPC IPC(8): A01G22/00A01G7/06A01G24/12A01G24/28C05F11/08C12N1/02
CPCA01G7/06A01G22/00A01G24/12A01G24/28C05F11/08C12N1/02
Inventor 刘涛字淑慧廖秋红杨生超李艳冰徐若李莎金鑫
Owner YUNNAN AGRICULTURAL UNIVERSITY
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