The invention relates to medicinal liquor capable of killing bacteria, dispelling wind, removing obstruction in meridians and relieving pain and a preparation method thereof. The medicinal liquor is composed of, by weight, grasshoppers, sanchi, scorpions, centipedes, squama manis, chuanxiong rhizomas, tuberculate speranskia herbs, herba lycopodii, ground beetles, willow branches, rhizoma phragmitis, starfishes, Chinese prickly ash, Sichuan pepper, mature ginger, turtle shells, bulbus allii, rhizoma gastrodiae and bark of Japanese cinnamon. The medicinal materials are immersed in baijiu, so that the medicinal liquor is obtained. The medicinal liquor has the effects of dispelling wind, eliminating dampness, dispelling cold, relaxing tendons, dredging collaterals, promoting blood circulation to remove blood stasis, promoting qi circulation to relieve pain, removing toxic materials, eliminating stagnation, eliminating swelling, expelling pus, diminishing inflammation and killing bacteria, can promote blood circulation, restore joint and cartilage injury, eliminate infection cells and restore healthy skin and is effective for various bone joint diseases, various skin diseases, snake bites, haemorrhoids and gynecologic inflammation, the total effective rate reaches 97.02%, and the cure rate reaches 60.71%.