411 results about "Hemolysis" patented technology

The invention relates to the technical field of blood potassium detection, in particular to a blood potassium detection paper-based sensor and a preparation method and application thereof.The sensor is obtained by integrating a substrate, a sensing electrode and a whole blood microfluid channel, and the sensing electrode is located between the substrate and the whole blood microfluid channel; the whole blood microfluid channel comprises a serum diffusion area, a whole blood separation area positioned above the serum diffusion area and a blood potassium detection area positioned below the serum diffusion area; a blood separation membrane is also arranged in the whole blood separation area; the substrate is qualitative filter paper, and the sensing electrode comprises an all-solid-state PBA working electrode and an Ag / AgCl reference electrode; compared with the prior art, calibration is not needed when potassium ions in whole blood are detected, the whole blood sample can be directly used, centrifugal separation is not needed, the detection speed is increased, the hemolysis problem of the whole blood sample in blood potassium detection can be avoided, and the detection sensitivity, accuracy and stability are further improved.

The invention provides a sample pretreatment automation method for flow cytometry, and belongs to the technical field of sample pretreatment for flow cytometry. A biological sample to be detected is collected and placed in a collection tube containing an EDTA-K2 anticoagulant for pretreatment; an automatic pipetting system is used for carrying out incubation reaction on an anticoagulant whole blood sample and an intelligent matching fluorescence labeling antibody mixed solution, and a double-layer game optimization verification system is used for verifying the rationality of an antibody adding proportion and reaction condition parameters; carrying out fluorescence characteristic readability index prediction on the incubated cell suspension by adopting an intelligent dilution parameter regulator model, dynamically adjusting hemolysis parameters and suspension conditions, and adding 1 * hemolysin working solution by using an automatic liquid adding device to carry out hemolysis treatment so as to finish pretreatment; the technical problems that in the pretreatment process of flow cytometry detection samples, parameters are often based on artificial experience, and the reproducibility and standardization degree of detection results are insufficient are solved.

The invention belongs to the technical field of blood detection, and particularly relates to an experimental animal blood detection device which comprises a detector body and a sample injection needle, and further comprises a sample mixing part, and the sample mixing part comprises a disc rotationally installed on the detector body; the fixing bases are installed on the disc, and clamping assemblies are rotationally installed on the fixing bases; the driving assembly is installed on the fixing base and comprises a main shaft rotationally installed on the fixing base and an auxiliary shaft used for driving the clamping jaw to rotate, a plurality of sets of auxiliary gears and main gears are installed on the auxiliary shaft and the main shaft respectively, the tooth ratios of the auxiliary gears and the main gears in each set are different, and the auxiliary shaft is of a telescopic structure; by arranging the driving assembly, different shaking speeds are adopted for blood collection tubes of different specifications, it is ensured that blood and an anticoagulant are evenly mixed, the situation of hemolysis or uneven mixing is avoided, and the detection accuracy and efficiency are improved.

The invention discloses a method for controlling the compaction degree of a left heart and a right heart in an extracorporeal circulation operation, which comprises the following steps of: in an in-vitro environment, mounting a peristaltic pump at a left heart inlet and a right heart inlet of an extracorporeal circulation device; a liquid inlet of the peristaltic pump is connected with a liquid inlet pump, and a liquid outlet of the peristaltic pump is connected with a liquid outlet pump; and finally, a first pressure measuring structure and a second pressure measuring structure are arranged on the sides, away from the peristaltic pump, of the liquid inlet pump and the liquid outlet pump correspondingly, and the pressure of the liquid inlet pump and the pressure of the liquid outlet pump are adjusted to be-20 mmHg. The negative pressure of the liquid inlet pump is adjusted through the first pressure measuring structure, and the negative pressure of the liquid outlet pump is adjusted through the second pressure measuring structure, so that the purpose of quantifying the adjusting mode is achieved, and the adjusting mode is accurate, rapid and high in reliability; the peristaltic pump can effectively avoid the occurrence of risks such as blood component damage, hemolysis, gas embolism and the like caused by reduction of the amount of blood conveyed by the pump, insufficient blood supply or direct interruption of blood circulation due to the condition that a pipe body connected with the peristaltic pump is shrunken or cannot be sucked during an operation.

The invention belongs to the technical field of gene engineering, and particularly relates to ALFPm3 modified antibacterial peptide, a molecular design and construction method and application of the ALFPm3 modified antibacterial peptide, and the amino acid sequence of the ALFPm3 modified antibacterial peptide is selected from any one of SEQ ID NO: 1-SEQ ID NO: 7. Analysis and design are carried out by combining the structure and action site of vibrio parahaemolyticus and the molecular structure and function relationship of the antibacterial peptide ALFPm3 as a basic template, the antibacterial activity of the antibacterial peptide ALFPm3 is improved, and other biological activities such as hemolysis are reduced.

The invention belongs to the field of polypeptides, and particularly relates to an antibacterial peptide derived from marine microorganisms, an antibacterial composition and application. Based on a deep learning algorithm, five potential antibacterial peptides are mined from a global marine microbiome database, and the five antibacterial peptides are expressed in yarrowia lipolytica. Experimental tests show that the five antibacterial peptides have differentiated antibacterial effects on different bacteria, and the antibacterial peptide shown in SEQ ID NO: 3 can have an obvious inhibition effect on escherichia coli, pseudomonas aeruginosa and staphylococcus aureus, has low hemolysis and has a positive application prospect. The antibacterial peptide disclosed by the invention has the potential of further developing novel antibacterial drugs or antibacterial additives.

The invention belongs to the technical field of polypeptides and biological medicines, and particularly relates to a WK7 series antibacterial peptide and application thereof in the aspects of resisting bacteria and preventing and treating diabetic foot, pneumonia and septicemia. According to the invention, a linear peptide WK7-line with an amino acid sequence of WKRWKRW is used as a minimum active unit, and modification is carried out to form a plurality of linear modified peptides; meanwhile, a cyclic peptide WK7-cyl is formed on the basis of the linear peptide WK7-line through cyclization, and the cyclic peptide WK7-cyl is further modified to form an annular modified peptide. The linear peptide WK7-line, the linear modified peptide, the cyclic peptide WK7-cyl and the cyclic modified peptide jointly form the WK7-series antibacterial peptide, and the WK7-series antibacterial peptide is artificially synthesized polypeptide, is small in molecular weight, easy to synthesize, free of cytotoxicity, almost free of hemolysis risk, high in sterilization speed and broad-spectrum antibacterial property and can be used for preparing the antibacterial peptide. The traditional Chinese medicine composition has a certain effect on prevention and treatment of diabetic foot ulcer, pneumonia and septicemia.

The invention discloses application of fermented lactobacillus mucus capable of prolonging life, resisting aging and resisting oxidation, and belongs to the technical field of functional strains. The preservation numbers of the lactobacillus mucus A21224 and the lactobacillus mucus A21227 are GDMCC No: 62958 and GDMCC No: 62962 respectively, and the lactobacillus mucus A21224, the lactobacillus mucus A21227 and the lactobacillus mucus A21227 are used for The two strains can remarkably prolong the service life of nematodes, have good antioxidant effect and heat stress resistant effect, can prolong the service life of the nematodes by 40% in a strong oxidation environment, and can prolong the service life of the nematodes by 28.57% under the stress of a thermal environment. In addition, the lactobacillus mucus A21224 and the lactobacillus mucus A21227 both show an excellent anti-aging effect in the late stage of the nematodes. Meanwhile, the two strains have certain gastrointestinal fluid-resistant and cholate-resistant effects, can be colonized in intestinal tracts, have no hemolysis phenomenon and are high in safety.

The invention belongs to the field of microorganisms, and provides lactobacillus paracasei Jlus660321 with a function of assisting in reducing blood fat and cholesterol and application of the lactobacillus paracasei Jlus660321, and the preservation number of the lactobacillus paracasei Jlus660321 is CGMCC No. 33962. The lactobacillus paracasei Jlus 660321 has the functions of assisting in lowering blood fat and lowering cholesterol, shows excellent cholesterol lowering performance in a cholesterol degradation experiment, and can degrade 61.60% of cholesterol in the experiment. A hemolysis experiment result is gamma-hemolysis, namely, hemolysis is avoided, and toxic and side effects are small. Rat in-vivo experiments show that the lactobacillus paracasei Jlus 660321 can effectively slow down the increase of body weight, liver weight, liver index, low-density lipoprotein content and triglyceride content caused by high-fat diet, and further show that the lactobacillus paracasei Jlus 660321 has the function of assisting in reducing blood fat and cholesterol; the compound can be used for preparing medicines, nourishment or health care products for assisting in reducing blood fat and / or reducing cholesterol.

The invention discloses a blood bag continuous extrusion method and device, a medium and equipment. The method comprises the steps that the blood bag continuous extrusion process is divided into a plurality of extrusion stages; obtaining a target extrusion parameter, and generating a target pressure value corresponding to each extrusion stage; and the current extrusion pressure is collected, the current extrusion pressure is adjusted to the corresponding target pressure value based on the preset control scheme of each extrusion stage, and continuous extrusion is conducted till the preset extrusion ending condition is met. The extrusion process is divided into a plurality of stages, the actual extrusion pressure of a target monitoring point on a mother bag serves as system input, the running speed of a stepping motor is adjusted in real time through a PID algorithm, and therefore the whole extrusion process is controlled to be within a proper pressure range; and after the pressure reaches the target value, the whole extrusion process is subjected to pressure maintaining according to the set small target pressure, so that the preparation efficiency is high, the hemolysis phenomenon is avoided, the quality of the separation process and the product percent of pass are improved, and the risk of abnormal quality is reduced.

The invention provides a silk fibroin hemostatic sponge loaded with polyhydroxy phenolic compounds and a preparation method of the silk fibroin hemostatic sponge, aiming at the defect that hemostatic materials have poor effects on large wounds and serious bleeding, and the silk fibroin hemostatic sponge loaded with the polyhydroxy phenolic compounds is prepared from silk fibroin compounds loaded with the polyhydroxy phenolic compounds, wherein the silk fibroin compound loaded with the polyhydroxy phenolic compound is prepared from the polyhydroxy phenolic compound and silk fibroin according to the feeding mass ratio of 1t; the material is prepared in a 1: 1 self-assembly mode. The polyhydroxy phenolic compound is preferably insoluble and most preferably ellagic acid, and the feeding ratio of the polyhydroxy phenolic compound to the silk fibroin is preferably 1: 2-1: 16 and more preferably 1: 4. The hemostatic capacity of the silk fibroin compound loaded with the polyhydroxy phenolic compound is remarkably superior to that of silk fibroin, Yunnan Baiyao, tranexamic acid, chitosan hemostatic powder and the like, and the silk fibroin compound is free of cytotoxicity, free of hemolysis, capable of being self-degraded and high in safety. The silk fibroin hemostatic sponge loaded with the polyhydroxy phenolic compound has excellent hemostatic performance, is easy to peel, does not cause secondary damage, and can be self-degraded.

The double-cavity cannula comprises a cannula body, the cannula body comprises an outer tube with an opening in the upper end and an inner tube coaxial with the outer tube, the upper end of the outer wall of the cannula body is provided with a connecting part communicated with the cannula body in a matched mode, a plurality of blood guide side holes are formed in the lower portion of the outer wall of the outer tube, and the opening in the lower end of the inner tube is communicated with a blood guide hopper. A blood return assembly is arranged on the inner wall of the outer tube and above the blood guide hopper, a blood flow guide cover is arranged on the inner wall of a blood return opening of the blood return assembly in a matched mode, a balloon capable of being filled and emptied is arranged on the outer wall of the outer tube and above the blood guide side hole, a filling valve is arranged on one side of the outer wall of the connecting part, and the filling valve is communicated with the balloon through a filling pipeline. When blood is guided, the conditions of local blood vessel wall adsorption and unsmooth blood guiding cannot be easily caused by the design of the blood guiding side holes, and the injection effect, blood turbulence and hemolysis risk increase cannot occur during blood return.

The invention relates to the technical field of microorganisms, and discloses Ackermann muciniphila, a combined microbial agent thereof and application of the Ackermann muciniphila in inflammatory enteritis. The invention provides Akkermansia muciniphila AIML-559, and the preservation number of the Akkermansia muciniphila AIML-559 is CGMCC (China General Microbiological Culture Collection Center) No. 34246. The invention relates to a combined microbial agent, which is characterized by comprising Ackermann muciniphila AIML-559 and Bifidobacterium adolescentis AIML-528 (the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.34300). The combined microbial agent is prepared from the following raw materials: Ackermann muciniphila AIML-559 and Bifidobacterium adolescentis AIML-528 The ackermann mucophil combined microbial inoculum is suitable for treatment intervention of inflammatory bowel disease (IBD), and can relieve weight loss, hemolysis, anemia, diarrhea, intestinal bleeding and other symptoms of IBD mice.

Provided are methods for treatment of pathological conditions causing hemolysis, cerebral edema, acute kidney injury and non-anaphylactic shock, including envenomation, trauma, cerebral malaria and mast-cell diseases using at least one PLA2 inhibitor, alone or in combination with other agents. The unexpected versatility of PLA2 inhibitors, their dosage forms and combinations make them candidates as essential medicines for the developing world.

The invention discloses a QS-21 composite adjuvant and a preparation method thereof, and belongs to the technical field of biological medicines. According to the QS-21 composite adjuvant, a polyanion material and QS-21 are compounded to form nanoparticles, and the nanoparticles are organically combined with a freeze-drying protective additive, so that the prepared QS-21 composite adjuvant is relatively high in retention rate, the problems of hydrolysis and oxidation are solved, the requirement of a phospholipid carrier is avoided, and a vaccine co-preparation process is simplified; the subsequently added freeze-drying protective agent retains the immune enhancement activity of the QS-21 and reduces the exposure of the QS-21 to a degradation environment; the corresponding cytotoxicity meets the standard, and the hemolysis rate is low; therefore, the method has remarkable clinical application value and industrialization potential.

A system for treatment of patient tissue by delivery of high-voltage pulses comprising an ablation catheter, a measurement unit and an electronic control unit (ECU). The measurement unit is configured to perform measurements using an energy source, whereby the impedance and / or current measurement values are determined as response to an alternating voltage and / or at least one voltage pulse. The ECU is configured to receive and analyze said measurement values provided by the measurement unit and identify a presence of hemolysis based on a determined decrease in the measured impedance values over time.

The invention belongs to the field of microorganisms, and particularly relates to lactococcus lactis Jlus 880314 and application thereof. The strain is classified and named as Lactococcus lactis, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing; the preservation number of the strain is CGMCC No. 33961. Time-of-flight mass spectrometry analysis shows that nisin exists in a metabolite of the strain, and the strain can be used for producing nisin. The compound shows an excellent and wide antibacterial function in antibacterial experiments, can be used for preparing antibacterial drugs, particularly has a remarkable inhibiting effect on growth of gram-negative bacteria and fungi, can be used for preparing drugs for relieving or treating enteritis, or preparing drugs, nourishment or health care products for regulating gastrointestinal tract micro-ecological balance of people or animals, and can be used for preparing medicines for relieving or treating enteritis. The compound can also be used for preparing antibacterial food additives and / or animal feed additives. A hemolysis experiment result shows that hemolysis is avoided, toxic and side effects are small, and wide development prospects and application values are achieved.

The invention belongs to the technical field of vaccine adjuvants, and particularly relates to tubeimoside B liposome as well as a preparation method and application thereof. The preparation method comprises the following steps: dissolving phospholipid, cholesterol and PEGylated phospholipid in an organic solvent to form a mixed solution, and then evaporating the mixed solution to form a film; and hydrating the film by using an aqueous solution of tubeimoside B to finally prepare the tubeimoside B liposome. Compared with free tubeimoside B, the lipidosome has the advantages that the hemolysis percentage is obviously reduced, and antigen-specific humoral immune response and / or cellular immune response can be effectively enhanced when the lipidosome is used as a vaccine adjuvant. In an H1N1 influenza mouse model, the lipidosome combined antigen shows an excellent protection effect, which is shown in that the mouse weight fluctuation is relatively small and the lung virus load is remarkably reduced. The invention provides important theoretical basis and practical examples for developing low-toxicity and high-efficiency plant-derived vaccine adjuvants, and has a wide application prospect.

The invention relates to application of a covalent organic framework material in preparation of a blood perfusion adsorbent, and the covalent organic framework material not only can adsorb toxins in blood with high adsorption capacity, but also has the advantages of excellent adsorption selectivity, low hemolysis rate, protein adhesion resistance and the like.

The invention relates to a chemiluminescence kit for detecting pepsinogen II. The kit comprises an enzyme marker, a luminescent substrate solution and an anti-interference buffer system, the anti-interference buffer system comprises a nonionic surfactant, animal-derived IgG (immunoglobulin G), an antioxidant and catalase; the chemiluminescence kit also comprises a PG II calibration product and a PG II quality control product. The core active component of the enzyme marker is beta-galactosidase. The enzyme marker further comprises an anti-PG II monoclonal antibody marked by beta-galactosidase, bovine serum albumin, trehalose, cane sugar, MgCl2. 6H2O, sodium chloride, Proclin300 and a phosphate buffer solution. According to the kit, a brand-new anti-interference buffer system is introduced on the basis of a spatial proximity chemiluminescence analysis method, so that the resistance to clinically common interferents (such as hemolysis, lipemia, jaundice and heterotropism antibodies) is remarkably improved, and the detection result is more accurate and reliable.

The embodiment of the invention provides a hemolysis risk assessment method and system based on erythrocyte morphology, and belongs to the technical field of biomedical treatment. The method comprises the following steps: carrying out image segmentation on a blood sample smear image to obtain a corresponding target segmentation result; performing target classification on the target segmentation result according to the first reference image and the second reference image to obtain a first classification result corresponding to the first reference image and a second classification result corresponding to the second reference image; calculating the difference degree between the first classification result and the second classification result to obtain a difference value; determining a target classification result of the target segmentation result by using the first classification result and the second classification result according to the difference value; determining a first number corresponding to normal erythrocytes and a second number corresponding to abnormal erythrocytes in the target object according to the target classification result; and performing hemolysis risk assessment on the target object according to the first quantity, the second quantity, the blood sample smear image and the blood item test parameters to obtain a target assessment result corresponding to the target object.

The invention discloses an antibacterial peptide IFD targeting gram-negative bacteria as well as a preparation method and application thereof. The amino acid sequence of the antibacterial peptide IFD is shown as SEQ NO.1, and a C terminal is amidated by adopting-NH2. The antibacterial peptide is obtained by modifying 119-132 fragments of human-derived MD-2 protein, and is prepared by site-specific mutagenesis, hydrophobic and hydrophilic sequence insertion optimization and solid-phase chemical synthesis. The antibacterial peptide has high antibacterial activity on Gram-negative bacteria (such as escherichia coli and salmonella typhimurium), the GM Gram-negative bacteria MIC reaches 3.63 mu M and is increased by 5.24 times compared with the original peptide, the targeting property is high, and the hemolysis rate 1t when the hemolysis activity is 128 mu M is low; 3%), the Gram-negative bacteria therapeutic index is as high as 76.1, and the Gram-negative bacteria can be used as a feed antibiotic substitute for preparing drugs for treating Gram-negative bacteria infectious diseases.

The embodiment of this specification relates to a position-controlled suspension centrifugal blood pump, which is a "pump-motor integrated" device. The blood pump includes: a pump housing, a rotor, an impeller, a servo motor, an inner magnetic core group, an outer magnetic ring group, and a position-control bearing; the rotor and the impeller are seamlessly connected. The rotor is arranged in the inner tube of the blood pump and is driven by the servo motor to drive the impeller to do work; the inner magnetic core group is composed of multiple round magnetic pieces and is built in the top end inside the rotor. The outer magnetic ring group is composed of multiple magnetic rings and is sleeved on the outer wall of the inner tube; the position-control bearing is composed of ceramic balls and ceramic sockets. The top end of the impeller blade has an inclined surface, which can generate hydrodynamic pressure when the pump is working. According to the technical solution of the present invention, the rotor and the impeller can be position-controlled and suspended in all directions, overcoming the friction effect of mechanical bearings. The internal structure of the pump is simple and smooth, which can greatly reduce hemolysis and thrombus complications; the blood pump is small in size and light in weight, with less surgical invasiveness, and can improve safety and practicability.

The invention provides a strain of high-temperature-resistant and inflammation-relieving Witzia ciliaris and an application thereof, the high-temperature-resistant and inflammation-relieving Witzia ciliaris is Witzia ciliaris CN985 and is preserved in the China Center for Type Culture Collection (CCTCC), the preservation date is April 7, 2024, and the preservation number is CCTCC NO: M 2024641. The invention further provides a preparation method of the high-temperature-resistant and inflammation-relieving Witzia ciliaris strain for preventing and treating inflammation of the high-temperature-resistant and inflammation-relieving Witzia ciliaris strain for preventing and treating inflammation of the high-temperature-resistant and inflammation-relieving Witzia ciliaris strain. The invention provides a brand new strain of Witzia ciliaris CN985, which is resistant to high temperature, has better acid resistance and bile resistance, and has no hemolysis risk when being applied to a human body. The Witzia coagulans CN985 has excellent antibacterial performance, and especially can significantly inhibit streptococcus pyogenes, salmonella enteritidis and / or group B streptococcus; besides, experimental results show that the Witzia ciliaris CN985 can relieve enteritis, improve the immunity of the organism and relieve pharyngitis, and especially when the Witzia ciliaris CN985 and the phytobacterium plantarum CE18 are combined for use, the Witzia ciliaris CN985 and the phytobacterium plantarum CE18 have a synergistic interaction effect, so that the relieving effect on enteritis can be remarkably improved.

The invention discloses a high-oxygen-permeability membrane material and a preparation method thereof, and belongs to the technical field of membrane materials. The membrane material is prepared by taking polydimethylsiloxane and polycaprolactone as organic raw materials, adding an inorganic material nano titanium dioxide or graphene, and carrying out solution preparation, cross-linking reaction, casting membrane formation, hot press molding, plasma treatment and surface modification. The polydimethylsiloxane guarantees the oxygen permeability, the polycaprolactone improves the flexibility and the biocompatibility, and the nano inorganic particles enhance the mechanical strength and the antibacterial property. The prepared membrane material is improved in oxygen permeability coefficient, greatly improved in tensile strength and elongation at break, good in hydrophilicity and biocompatibility, low in cytotoxicity and small in hemolysis rate, can be widely applied to medical products such as artificial lungs, wound dressings and contact lenses, and effectively meets the requirements of the medical field for the membrane material with high oxygen permeability and good comprehensive performance.

An implantable pump system is provided, including an implantable blood pump suitable for use as a partial support assist device, the system further including an extracorporeal battery and a controller coupled to the implantable pump, and a programmer selectively periodically coupled to the controller to configure and adjust operating parameters of the implantable pump. The implantable pump includes a flexible membrane coupled to an electromagnetic actuator including a magnetic assembly and electromagnetic assembly, so that when the electromagnetic assembly is energized, the electromagnetic assembly causes wavelike undulations to propagate along the flexible membrane to propel blood through the implantable pump. The controller may be programmed by a programmer to operate at frequencies and duty cycles that mimic physiologic flow rates and pulsatility while operating in an efficient manner that avoids thrombus formation, hemolysis and / or platelet activation.

A blood testing device for detecting hemolysis in a blood sample is described. The blood testing device comprises an housing for containing the blood sample. The housing has a treatment window and an optical zone formed therein. The blood testing device further includes an acoustic transducer positioned to selectively generate acoustic forces directed into the treatment window of the housing and a control unit for selectively actuating and deactuating the acoustic transducer to permit colorimetric analysis of plasma within the blood sample.

The invention discloses an antibacterial peptide PA targeting multi-drug-resistant gram-negative bacteria and application of the antibacterial peptide PA, and the amino acid sequence of the antibacterial peptide PA is shown as SEQ ID NO.1. The antibacterial peptide PA provided by the invention has a remarkable antibacterial effect of the multi-drug-resistant gram-negative bacteria, is quick in bactericidal effect, does not have hemolysis and cytotoxicity, does not cause renal toxicity after treatment, and can be used for preparing the antibacterial peptide PA for treating the multi-drug-resistant gram-negative bacteria. The biological safety is good, and the compound can be used for treating bacterial infection and can also be applied to other scenes needing sterilization or bacterial growth inhibition.

The invention relates to the technical field of microorganisms, in particular to kitten source lactobacillus reuteri L50 and application thereof. The invention provides a kitten source lactobacillus reuteri L50, the collection number of the kitten source lactobacillus reuteri L50 is CGMCC (China General Microbiological Culture Collection Center) NO.31003, the kitten source lactobacillus reuteri L50 is collected in the China General Microbiological Culture Collection Center, the collection date is July 1, 2024, and the collection address is Institute of Microbiology, Chinese Academy of Sciences, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, China. The provided kitten-source lactobacillus reuteri L50 is a bacterial strain which is separated, screened and identified from kitten feces and has excellent probiotic performance, is high in adhesion capacity, has good probiotic capacity, acid resistance and cholate resistance, has a good inhibition effect on common pathogenic bacteria, is sensitive to various antibiotics, is free of hemolysis, is high in safety, and can be used for preparing the kitten-source lactobacillus reuteri L50 for the kitten-source lactobacillus reuteri L50 for the kitten-source lactobacillus reuteri L50 for the kitten-source lactobacillus reuteri L50. Wide application is facilitated.

The invention belongs to the technical field of gene engineering, and particularly relates to ALFPm3 modified antibacterial peptide, a molecular design and construction method and application of the ALFPm3 modified antibacterial peptide, and the amino acid sequence of the ALFPm3 modified antibacterial peptide is selected from any one of SEQ ID NO: 1-SEQ ID NO: 7. Analysis and design are carried out by combining the structure and action site of vibrio parahaemolyticus and the molecular structure and function relationship of the antibacterial peptide ALFPm3 as a basic template, the antibacterial activity of the antibacterial peptide ALFPm3 is improved, and other biological activities such as hemolysis are reduced.