151 results about "Hemolysis" patented technology

The invention belongs to the technical field of polypeptides and biological medicines, and particularly relates to a WK7 series antibacterial peptide and application thereof in the aspects of resisting bacteria and preventing and treating diabetic foot, pneumonia and septicemia. According to the invention, a linear peptide WK7-line with an amino acid sequence of WKRWKRW is used as a minimum active unit, and modification is carried out to form a plurality of linear modified peptides; meanwhile, a cyclic peptide WK7-cyl is formed on the basis of the linear peptide WK7-line through cyclization, and the cyclic peptide WK7-cyl is further modified to form an annular modified peptide. The linear peptide WK7-line, the linear modified peptide, the cyclic peptide WK7-cyl and the cyclic modified peptide jointly form the WK7-series antibacterial peptide, and the WK7-series antibacterial peptide is artificially synthesized polypeptide, is small in molecular weight, easy to synthesize, free of cytotoxicity, almost free of hemolysis risk, high in sterilization speed and broad-spectrum antibacterial property and can be used for preparing the antibacterial peptide. The traditional Chinese medicine composition has a certain effect on prevention and treatment of diabetic foot ulcer, pneumonia and septicemia.

The double-cavity cannula comprises a cannula body, the cannula body comprises an outer tube with an opening in the upper end and an inner tube coaxial with the outer tube, the upper end of the outer wall of the cannula body is provided with a connecting part communicated with the cannula body in a matched mode, a plurality of blood guide side holes are formed in the lower portion of the outer wall of the outer tube, and the opening in the lower end of the inner tube is communicated with a blood guide hopper. A blood return assembly is arranged on the inner wall of the outer tube and above the blood guide hopper, a blood flow guide cover is arranged on the inner wall of a blood return opening of the blood return assembly in a matched mode, a balloon capable of being filled and emptied is arranged on the outer wall of the outer tube and above the blood guide side hole, a filling valve is arranged on one side of the outer wall of the connecting part, and the filling valve is communicated with the balloon through a filling pipeline. When blood is guided, the conditions of local blood vessel wall adsorption and unsmooth blood guiding cannot be easily caused by the design of the blood guiding side holes, and the injection effect, blood turbulence and hemolysis risk increase cannot occur during blood return.

The invention provides a strain of high-temperature-resistant and inflammation-relieving Witzia ciliaris and an application thereof, the high-temperature-resistant and inflammation-relieving Witzia ciliaris is Witzia ciliaris CN985 and is preserved in the China Center for Type Culture Collection (CCTCC), the preservation date is April 7, 2024, and the preservation number is CCTCC NO: M 2024641. The invention further provides a preparation method of the high-temperature-resistant and inflammation-relieving Witzia ciliaris strain for preventing and treating inflammation of the high-temperature-resistant and inflammation-relieving Witzia ciliaris strain for preventing and treating inflammation of the high-temperature-resistant and inflammation-relieving Witzia ciliaris strain. The invention provides a brand new strain of Witzia ciliaris CN985, which is resistant to high temperature, has better acid resistance and bile resistance, and has no hemolysis risk when being applied to a human body. The Witzia coagulans CN985 has excellent antibacterial performance, and especially can significantly inhibit streptococcus pyogenes, salmonella enteritidis and / or group B streptococcus; besides, experimental results show that the Witzia ciliaris CN985 can relieve enteritis, improve the immunity of the organism and relieve pharyngitis, and especially when the Witzia ciliaris CN985 and the phytobacterium plantarum CE18 are combined for use, the Witzia ciliaris CN985 and the phytobacterium plantarum CE18 have a synergistic interaction effect, so that the relieving effect on enteritis can be remarkably improved.

The invention discloses an antibacterial peptide PA targeting multi-drug-resistant gram-negative bacteria and application of the antibacterial peptide PA, and the amino acid sequence of the antibacterial peptide PA is shown as SEQ ID NO.1. The antibacterial peptide PA provided by the invention has a remarkable antibacterial effect of the multi-drug-resistant gram-negative bacteria, is quick in bactericidal effect, does not have hemolysis and cytotoxicity, does not cause renal toxicity after treatment, and can be used for preparing the antibacterial peptide PA for treating the multi-drug-resistant gram-negative bacteria. The biological safety is good, and the compound can be used for treating bacterial infection and can also be applied to other scenes needing sterilization or bacterial growth inhibition.

This invention discloses an antimicrobial peptide PA targeting multidrug-resistant Gram-negative bacteria and its application. The amino acid sequence of the antimicrobial peptide PA is shown in SEQ ID NO.1. The antimicrobial peptide PA provided by this invention has a significant antibacterial effect against multidrug-resistant Gram-negative bacteria, and has a rapid bactericidal onset, no hemolysis or cytotoxicity, does not cause nephrotoxicity after treatment, and has good biosafety. It can be used for the treatment of bacterial infections and can also be applied to other scenarios that require bactericidal or antibacterial growth inhibition.

The application discloses a hemolysis analyzer based on an integrated optical waveguide sensor and a preparation method thereof, and comprises an integrated photon waveguide, a nano filter and a micro-fluidic channel, wherein the integrated photon waveguide is a single waveguide with a middle spiral type and coupling gratings on both sides. The hemolysis analyzer fully utilizes the advantages of high sensitivity and small volume of the integrated optical waveguide sensor, and realizes sample-free preparation by combining the methods of instantaneous absorption and hemoglobin filtration, and can quickly and accurately detect hemolysis and the degree of hemolysis.

The invention relates to the technical field of biological pharmacy, and particularly discloses application of antibacterial small molecule peptide in preparation of antibacterial infection drugs. The antibacterial small molecule peptide is VeAMP, and the amino acid sequence of the antibacterial small molecule peptide is MKIVKRILLVL-NH2. The small molecule peptide VeAMP provided by the invention shows remarkable antibacterial activity on pan-drug-resistant acinetobacter baumannii, has the advantages of rapid sterilization, low hemolysis, low cytotoxicity and good in-vivo safety, is not easy to induce bacterial drug resistance after being exposed for a long time at a sub-inhibitory concentration, can effectively reduce tissue bacterial load, remarkably improves the survival rate of infected mice, and has a good application prospect. The invention provides a novel and safe candidate antibacterial agent which is not easy to generate drug resistance for preventing and treating drug-resistant acinetobacter baumannii infection.

The invention provides a carotid artery thrombus treatment catheter and a suction system comprising the same, and belongs to the technical field of medical instruments. The treatment catheter comprises a catheter body, a blocking balloon, a balloon pressurizing cavity and a Luer connector. The suction system comprises a Y-shaped connector, a blood transfusion module and a pressure monitoring system. Gapless wall attachment of the blood vessel wall is achieved through the blocking balloon, thrombus residues are avoided, suction-instrument conveying integrated operation is achieved in cooperation with the Y-shaped connector, and the multi-instrument exchange process is simplified; the balloon pressurizing cavity can accurately control the plugging balloon to expand to block blood flow, and thrombus fragments are prevented from being migrated; the peristaltic pump and the filter assembly of the blood transfusion module are connected through the catheter inner cavity, thrombus is sucked, filtered blood is transfused back, blood loss is reduced, the hemolysis risk is reduced, and the technical problems of thrombus residues, large blood loss amount, tedious operation, insufficient safety and the like are solved. The thrombus is efficiently removed, blood loss is remarkably reduced, the process is simplified, and the treatment safety is guaranteed through pressure monitoring.

The application belongs to the technical field of antibacterial medicine, and particularly relates to a cinnamaldehyde-modified polypyridine ruthenium complex for inhibiting hemolysis, a preparation method and application thereof. The cinnamaldehyde-modified polypyridine ruthenium complex has a structure as shown in formula I. 1 , formula I 2 or formula I 3 The cinnamaldehyde-modified polypyridine ruthenium complex can effectively reduce the hemolysis of red blood cells caused by toxins released by Staphylococcus aureus, and the ruthenium complex does not induce the drug resistance of bacteria, and can significantly increase the sensitivity of Staphylococcus aureus to aminoglycoside antibiotics.

The application discloses a biomedical test strip detection data analysis method and system based on multiple algorithms. The method comprises the following steps: obtaining related data of a test paper to be detected, wherein the related data comprises T-line related data, B-line related data and C-line related data; verifying whether the lengths of three curves corresponding to the related data are consistent, if not, determining that the detection result is invalid, if yes, analyzing sample adding characteristics to determine whether there is sample adding sequence error or no sample adding; distinguishing sample types according to chromatography speed according to the related data; when the sample type is a whole blood type, calculating the length of a sample type identification point corresponding to three lines, adjusting and cutting off, and if the total length exceeds a set threshold, determining that a hemolysis phenomenon leads to an invalid detection result. The method of the application particularly focuses on processing the hemolysis phenomenon and other user operation related problems, thereby enhancing the reliability and efficiency of the overall detection system.

The application discloses a sample information identification method, a computer readable storage medium and an identification device. The sample information identification method comprises the following steps: transporting a sample tube to a first identification area by controlling a transportation guide rail, acquiring sample tube information by a sample tube information reading mechanism; controlling an identification mechanism to capture a first image information of the sample tube; analyzing the first image information to acquire first identification information; comparing the first identification information with the sample tube information; if the first identification information matches the sample tube information, adjusting the position of a mechanical hand according to the first identification information to clamp the sample tube and move the sample tube to a second identification area; and acquiring sample information of the sample tube located in the second identification area. According to the sample information, whether the blood sample in the sample tube has hemolysis, jaundice and chyle phenomena and the severity information of the disease can be obtained, so that an abnormal sample with a relatively large influence on detection error can be identified.

The utility model provides a kind of blood specimen transmission cylinder, including main body cylinder, first buffer sleeve and cylinder cover;Wherein main body cylinder has accommodating cavity;First buffer sleeve is installed in accommodating cavity, blood collection tube can be inserted into first buffer sleeve;Cylinder cover can be connected with main body cylinder cover, after cover connection, the inner top wall of cylinder cover and the tube cap of blood collection tube abut.In transmission blood specimen, blood collection tube with blood specimen is placed in first buffer sleeve, then cylinder cover is connected with main body cylinder cover and fixed blood collection tube.The blood specimen transmission cylinder of the utility model can effectively avoid specimen spilling and hemolysis problem in transmission process, specifically, blood collection tube is placed in first buffer sleeve, the impact of transport vibration on blood collection tube is reduced using the buffer effect of sleeve, and the stability of tube body is enhanced, so as to prevent hemolysis;In addition, cylinder cover and main body cylinder are reliably fixed by clamping structure, and the pre-tightening pressure exerted on blood collection tube cap by cylinder cover inner wall can prevent tube cap from loosening and avoid spilling.

A method of characterizing a specimen to be analyzed in an automated diagnostic analysis system provides a segmentation determination and / or an HILN (hemolysis, icterus, lipemia, normal) determination of the specimen while providing characterization training updates based on the accuracy and / or confidence in the determinations. The method includes identifying an incorrect or low confidence segmentation or HILN determination, forwarding the incorrect or low confidence determination from the HILN network to a database, and providing one or more training images to the HILN network based on the incorrect or low confidence determination. Quality check modules and systems configured to carry out the method are also described, as are other aspects.

The invention relates to a preserving fluid for stabilizing viruses at normal temperature and high temperature as well as a preparation method and application of the preserving fluid. The preserving fluid is an aqueous solution containing the following components in concentration: 2mM-15mM of ethylenediamine tetraacetic acid tetrasodium salt, 5mM-20mM of sodium citrate, 0.1 wt%-10wt% of tween-20, 10wt%-30wt% of a structure stabilizer, 0.5 wt%-10wt% of an amide solution, 0.5 wt%-10wt% of a hemolysis inhibitor, 0.05 wt%-10wt% of a reducing agent and 0.1 mM-2mM of 3-morpholine propanesulfonic acid. According to the preservation solution, the formula is optimized, and particularly, the amide solution and the reducing agent component are matched with MOPS for use, so that the activity of viruses in a sample under normal-temperature and high-temperature conditions can be greatly maintained, and the stability of nucleic acid is effectively improved.

The application relates to the field of metal nanopowder or plasma spraying, and particularly discloses a biliary stent prepared by combining metal nanopowder with a plasma spraying process. The ZrNbFeLa coating prepared by the plasma spraying process has good corrosion resistance, but the hemolysis rate is poor. Further, a proper amount of metal Ti is injected into the ZrNbFeLa coating, so that the biocompatibility of the ZrNbFeLa coating can be significantly improved, the corrosion resistance is not greatly affected, and the ZrNbFeLa coating can meet the use requirements of medical materials such as the biliary stent. It should be noted that the ion injection amount of the metal Ti needs to be controlled within 2.8*10 5 ions / cm 2 -1.2*10 12 ions / cm 2 , otherwise, too much titanium will cause the corrosion resistance of the ZrNbFeLa coating to sharply decrease.

The application discloses a red blood cell room temperature storage solution and a preparation method and application thereof, and belongs to the technical field of biological medicines. The red blood cell storage agent comprises the following components in parts by weight: 1-200 parts of an anticoagulant, 1-200 parts of a blood cell stabilizer, 1-100 parts of a metabolism inhibitor, 1-100 parts of an electrolyte, 1-150 parts of an antioxidant, 350-1000 parts of deionized water, and pH 4.6-7.0. The components in the storage agent cooperate with each other and have a synergistic effect, and the multi-component synergistic protection can keep the red blood cell membrane integrity; the electrolyte balance osmotic pressure is maintained, the antioxidant removes free radicals and reduces hemolysis; the storage time is prolonged, the cell morphology and function are good; the cold chain dependence is reduced, and the demand for deep low-temperature equipment is reduced.

The invention provides an impeller state detection method and device, a blood pump, electronic equipment and a storage medium. The method is applied to the blood pump, the blood pump is a full magnetic suspension blood pump, the blood pump comprises a pump head assembly and a driving assembly, the pump head assembly comprises a pump shell and an impeller capable of being completely suspended in the pump shell, the driving assembly is connected with the pump head assembly, and the driving assembly magnetically drives the impeller to operate in the pump shell. Acquiring a mechanical wave signal which is acquired by a sensor and is generated when the impeller runs; and outputting alarm information corresponding to an abnormal operation event under the condition that the abnormal operation event of the impeller is detected based on the mechanical wave signal. By the adoption of the method and device, the abnormal condition that the impeller operates unstably in the early stage can be effectively recognized as early as possible, an alarm is given in time, the risks of supporting flow instability, pump body damage or hemolysis caused by continuous abnormal operation of the impeller are reduced, and the safety and reliability of operation of the blood pump are improved.

The invention belongs to the technical field of biological medicines, and particularly relates to an antibacterial peptide CeP28 from red deer as well as a preparation method and application thereof. The amino acid sequence of the antibacterial peptide CeP28 is shown as SEQ ID NO.1, and the antibacterial peptide CeP28 has good broad-spectrum antibacterial activity and has good antibacterial activity on gram-negative bacteria, gram-positive bacteria and fungi. Meanwhile, the antibacterial peptide CeP28 has low hemolysis activity on sheep red blood cells and low toxicity on mouse fibroblasts L929. Therefore, the antibacterial peptide CeP28 disclosed by the invention can be used as a potential broad-spectrum anti-infection drug.

Methods and systems configured to measure content of a target substance in a fluid sample using a refractometer including a light source and a light sensor. A method includes: calibrating the refractometer by identifying a calibration pixel pattern of first pixels of the light sensor illuminated by light from the light source that has reflected off a sensing surface of a container including a reference fluid having a known reference density; identifying a blood pixel pattern of second pixels of the light sensor illuminated by light from the light source that has reflected off the sensing surface when the fluid sample is present in the container; identifying a pixel shift distance along the light sensor from the calibration pixel pattern to the fluid pixel pattern; and determining the content of the target substance in the fluid sample based on the pixel shift distance.

An automatic standing device for blood collection tubes comprises a rack, a feeding unit, a code scanning unit and a conveying unit, the feeding unit, the code scanning unit and the conveying unit are installed on the rack, the feeding unit conducts discretized feeding on the blood collection tubes, the code scanning unit is used for obtaining information of the blood collection tubes, and the automatic standing device for the blood collection tubes further comprises an allocating unit and a standing cache unit; the conveying unit conveys the blood collection tubes to the allocating unit, the allocating unit shifts the blood collection tubes needing to be stood into the standing cache unit according to code scanning information, the standing cache unit comprises a cache bin, and the cache bin comprises a cache distribution box and at least two sequential cache bins arranged at equal arc intervals. Each temporary storage bin is defined by a temporary storage distribution box, blades and a visual door and window, all the blades are installed on a blade installation shaft, and the blade installation shaft is connected with a blade driving assembly used for driving the blades to rotate. On the premise that manual intervention is not needed, transmission stress is effectively relieved, the hemolysis rate is remarkably reduced, and meanwhile the continuity and high efficiency of an automatic process of a laboratory are guaranteed.

The application provides a spring tube, which comprises an inner layer tube, a spring and an outer layer tube arranged from inside to outside in sequence, the inner layer tube comprises a middle section and a hardened section arranged at least one end of the middle section and connected with the middle section, the end of the hardened section connected with the middle section is sleeved outside the middle section and connected with the middle section partially in overlap, and the inner wall of the hardened section is formed with a step; the spring is sleeved outside the inner layer tube and covers at least the middle section, the outer layer tube is sleeved outside the spring and covers the middle section and the hardened section, and the outer layer tube is connected with the hardened section in overlap. The application reduces the risk of blood injury and hemolysis.

The invention relates to an intrusive foldable left ventricle auxiliary device, the near end of a driving shaft is connected with a motor, the far end of the driving shaft is connected with a pump head assembly, and the pump head assembly comprises a foldable impeller driven by the driving shaft and an expandable support covering the periphery of the foldable impeller; the expandable stent is covered with a blood flow flexible film, a blood inlet is formed in the far end of the blood flow flexible film, and at least one blood outlet is formed in the near end area of the blood flow flexible film; the blood flow flexible membrane comprises a first channel, an expansion section and a second channel. When blood passes through the expansion section, the blood flow speed is reduced, the flow distribution is uniform, and the shearing force borne by the blood is directly reduced, so that the hemolysis risk is remarkably reduced from the source; meanwhile, blood flow is more stable, turbulent flow and flow stagnation areas are reduced, and the thrombosis risk is reduced; the near-end edge of the expansion section is located on the far side of the near-end edge of the expandable stent, after blood is decelerated, damage caused by impacting the inner wall of the stent is reduced, and hemolysis is further reduced.

A biosensor for detecting a hormone is provided. The biosensor for detecting a hormone can include a core component and a biological molecule (such as a receptor for the hormone or an antibody against the hormone) immobilized on the biosensor. Also provided are methods for detecting or quantitating a hormone in a sample using the biosensor provided herein. The biosensor can be a bio-layer interferometry (BLI) biosensor, and the signals generated by binding of a hormone to the biosensor can be indicative of spectral shift, which can be measured using BLI. Additional signals such as UV absorbance of the hormone or signals of a detectable label may be measured. The biosensor can detect the presence or amount of the hormone in a sample accurately without interference by the presence of biotin, hemolysis, or lipid in a sample.

The invention relates to the technical field of new drugs, in particular to a clevidipine butyrate concentrated solution and a preparation method thereof, and the composition contains clevidipine butyrate, a solubilizer, a cosolvent, a pH regulator and water for injection. Injection oil is abandoned, so that the requirements of lipid metabolism defect patients are met; no phospholipid is used in the prescription, safety risks such as hemolysis, immunoreaction and long-term toxicity and stability problems such as oxidative degradation are avoided, after phospholipid is removed, the clevidipine butyrate preparation can tolerate excessive hot-press sterilization, the sterility level guarantee degree is higher, the state of the clevidipine butyrate preparation is converted into a true solution system from a micelle system, and the stability of the clevidipine butyrate preparation is improved. The solubilizer and the cosolvent are combined, so that the solubility of clevidipine butyrate is greatly improved, dichloromethane, chloroform and other organic solvents with large toxic and side effects are not needed, the toxicity of the medicine is reduced, the stimulation to blood vessels is small, and the adverse reaction of the medicine is reduced; and the preparation method is simple and easy to amplify, and production amplification is facilitated by selecting a production process of a common injection.

The invention relates to the technical field of probiotics, in particular to intestinal tract source pediococcus acidilactici of Yanqu county in Tibet and fat reducing application thereof. The pediococcus acidilactici disclosed by the invention is preserved in the China General Microbiological Culture Collection Center on April 29, 2025, and the preservation number is CGMCC NO.34406; the strain has the characteristics of acid resistance, cholate resistance and stress resistance, and has high survival rate and planting efficiency; the strain is free of hemolysis, free of toxic factors and high in safety; the strain can secrete short-chain fatty acid and antibacterial peptide, inhibit pathogenic bacteria and promote intestinal barrier repair; short-chain fatty acid generated by metabolism of most of carbon water by the strain can be directly absorbed by intestinal epithelial cells, and can be used as an energy substance to replace partial glucose demand, so that the metabolism energy consumption is reduced, the energy conversion rate of feed is improved, the feed conversion is promoted, and the effects of reducing fat and weight and improving the non-alcoholic fatty liver disease are achieved.

The present invention is in the fields of antibody technology, medicine, pharmacology, infection biology, and medical diagnostics. More specifically, the present disclosure provides VHH antibodies that prevent membrane binding and / or oligomerization of Staphylococcus aureus (HLA) alpha-hemolysin and HLA-mediated hemolysis.

This invention discloses a method for crushing and purifying polyetheretherketone (PEEK), PEEK materials, and their applications. Belonging to the field of polymer composite materials technology, the method employs a process control approach using the polymerization mother liquor to regulate the polymer morphology in the polymerization solution, causing PEEK to precipitate in the form of spherical particles with a microspherical structure. Simple crushing yields low-particle-size ultrafine PEEK powder, with a particle size adjustable within the range of 8-100 micrometers and a high spheroidization rate. Simultaneously, the crushed low-particle-size ultrafine PEEK powder facilitates the purification of PEEK. Washing with organic solvents such as ethanol and acetone, and distilled water, PEEK powder can be obtained with high efficiency and cost-effectiveness. Due to the small particle size of PEEK, it is easy to purify. After simple purification, the cytotoxicity and hemolysis rate meet the standards for implantable medical-grade PEEK materials for human implantation, reducing the raw material costs of medical product manufacturing and enabling the general public to use more affordable and high-quality medical products.

This application discloses an adaptive oscillation method, system, and storage medium for blood products. The method includes: S1: acquiring and reconstructing multidimensional physical features of the blood bag surface to obtain three-dimensional morphological data and heat distribution data of the blood bag surface; S2: based on the three-dimensional morphological data and heat distribution data, using a fluid dynamics proxy model to inversely deduce the fluid shear stress and comprehensive physical suspension feature vector of the blood inside the blood bag, and performing cross-calibration on both, continuously outputting a mixed completion confidence score; S3: adjusting the motion parameters of the oscillation tray and clamping mechanism in real time according to the mixed completion confidence score, and stopping oscillation when the mixed completion confidence score continuously exceeds a preset threshold. This application achieves real-time, non-invasive sensing of the blood state inside a sealed blood bag, ensuring uniform suspension of red blood cells while avoiding mechanical hemolysis, significantly improving the safety, adaptability, and processing efficiency of blood product oscillation processing.

A blood collection device can include a syringe having hemolysis protection. The syringe with hemolysis protection can be coupled to a catheter assembly and used to draw blood. The blood collection device can include a syringe that can include a distal end and a flow restrictor. The flow restrictor can include a distal end and a proximal end. The proximal end of the flow restrictor can be coupled to the distal end of the syringe such that hemolysis can be protected against.

The invention relates to a blood sample cold storage transfer box with a temperature compensation function and a sample state real-time monitoring method, and belongs to the technical field of medical instruments and cold-chain logistics. The blood sample cold storage transfer box comprises a box body, a box cover, a heat preservation inner container, a phase change cold storage layer, a semiconductor chilling plate, a microcontroller, a temperature sensor and a sample state monitoring unit; the sample state monitoring unit comprises a light reflection type hemolysis detection module and an anti-leakage sensing layer. According to the invention, the microcontroller actively controls the semiconductor chilling plate to carry out refrigeration or heating compensation according to the feedback of the temperature sensor, and precise regulation and control of the temperature in the box are realized; meanwhile, the hemolysis state of the sample is monitored in a non-contact mode through an optical means, liquid leakage is monitored in real time through the conductive grid, the quality and biological safety of the blood sample in the transferring process are effectively guaranteed, and the whole-process traceability of the sample state is achieved.