281 results about "Hemolysis" patented technology

The invention provides a sample pretreatment automation method for flow cytometry, and belongs to the technical field of sample pretreatment for flow cytometry. A biological sample to be detected is collected and placed in a collection tube containing an EDTA-K2 anticoagulant for pretreatment; an automatic pipetting system is used for carrying out incubation reaction on an anticoagulant whole blood sample and an intelligent matching fluorescence labeling antibody mixed solution, and a double-layer game optimization verification system is used for verifying the rationality of an antibody adding proportion and reaction condition parameters; carrying out fluorescence characteristic readability index prediction on the incubated cell suspension by adopting an intelligent dilution parameter regulator model, dynamically adjusting hemolysis parameters and suspension conditions, and adding 1 * hemolysin working solution by using an automatic liquid adding device to carry out hemolysis treatment so as to finish pretreatment; the technical problems that in the pretreatment process of flow cytometry detection samples, parameters are often based on artificial experience, and the reproducibility and standardization degree of detection results are insufficient are solved.

The invention belongs to the field of polypeptides, and particularly relates to an antibacterial peptide derived from marine microorganisms, an antibacterial composition and application. Based on a deep learning algorithm, five potential antibacterial peptides are mined from a global marine microbiome database, and the five antibacterial peptides are expressed in yarrowia lipolytica. Experimental tests show that the five antibacterial peptides have differentiated antibacterial effects on different bacteria, and the antibacterial peptide shown in SEQ ID NO: 3 can have an obvious inhibition effect on escherichia coli, pseudomonas aeruginosa and staphylococcus aureus, has low hemolysis and has a positive application prospect. The antibacterial peptide disclosed by the invention has the potential of further developing novel antibacterial drugs or antibacterial additives.

The invention belongs to the technical field of polypeptides and biological medicines, and particularly relates to a WK7 series antibacterial peptide and application thereof in the aspects of resisting bacteria and preventing and treating diabetic foot, pneumonia and septicemia. According to the invention, a linear peptide WK7-line with an amino acid sequence of WKRWKRW is used as a minimum active unit, and modification is carried out to form a plurality of linear modified peptides; meanwhile, a cyclic peptide WK7-cyl is formed on the basis of the linear peptide WK7-line through cyclization, and the cyclic peptide WK7-cyl is further modified to form an annular modified peptide. The linear peptide WK7-line, the linear modified peptide, the cyclic peptide WK7-cyl and the cyclic modified peptide jointly form the WK7-series antibacterial peptide, and the WK7-series antibacterial peptide is artificially synthesized polypeptide, is small in molecular weight, easy to synthesize, free of cytotoxicity, almost free of hemolysis risk, high in sterilization speed and broad-spectrum antibacterial property and can be used for preparing the antibacterial peptide. The traditional Chinese medicine composition has a certain effect on prevention and treatment of diabetic foot ulcer, pneumonia and septicemia.

The double-cavity cannula comprises a cannula body, the cannula body comprises an outer tube with an opening in the upper end and an inner tube coaxial with the outer tube, the upper end of the outer wall of the cannula body is provided with a connecting part communicated with the cannula body in a matched mode, a plurality of blood guide side holes are formed in the lower portion of the outer wall of the outer tube, and the opening in the lower end of the inner tube is communicated with a blood guide hopper. A blood return assembly is arranged on the inner wall of the outer tube and above the blood guide hopper, a blood flow guide cover is arranged on the inner wall of a blood return opening of the blood return assembly in a matched mode, a balloon capable of being filled and emptied is arranged on the outer wall of the outer tube and above the blood guide side hole, a filling valve is arranged on one side of the outer wall of the connecting part, and the filling valve is communicated with the balloon through a filling pipeline. When blood is guided, the conditions of local blood vessel wall adsorption and unsmooth blood guiding cannot be easily caused by the design of the blood guiding side holes, and the injection effect, blood turbulence and hemolysis risk increase cannot occur during blood return.

The invention relates to the technical field of microorganisms, and discloses Ackermann muciniphila, a combined microbial agent thereof and application of the Ackermann muciniphila in inflammatory enteritis. The invention provides Akkermansia muciniphila AIML-559, and the preservation number of the Akkermansia muciniphila AIML-559 is CGMCC (China General Microbiological Culture Collection Center) No. 34246. The invention relates to a combined microbial agent, which is characterized by comprising Ackermann muciniphila AIML-559 and Bifidobacterium adolescentis AIML-528 (the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.34300). The combined microbial agent is prepared from the following raw materials: Ackermann muciniphila AIML-559 and Bifidobacterium adolescentis AIML-528 The ackermann mucophil combined microbial inoculum is suitable for treatment intervention of inflammatory bowel disease (IBD), and can relieve weight loss, hemolysis, anemia, diarrhea, intestinal bleeding and other symptoms of IBD mice.

The invention discloses a QS-21 composite adjuvant and a preparation method thereof, and belongs to the technical field of biological medicines. According to the QS-21 composite adjuvant, a polyanion material and QS-21 are compounded to form nanoparticles, and the nanoparticles are organically combined with a freeze-drying protective additive, so that the prepared QS-21 composite adjuvant is relatively high in retention rate, the problems of hydrolysis and oxidation are solved, the requirement of a phospholipid carrier is avoided, and a vaccine co-preparation process is simplified; the subsequently added freeze-drying protective agent retains the immune enhancement activity of the QS-21 and reduces the exposure of the QS-21 to a degradation environment; the corresponding cytotoxicity meets the standard, and the hemolysis rate is low; therefore, the method has remarkable clinical application value and industrialization potential.

The invention belongs to the field of microorganisms, and particularly relates to lactococcus lactis Jlus 880314 and application thereof. The strain is classified and named as Lactococcus lactis, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing; the preservation number of the strain is CGMCC No. 33961. Time-of-flight mass spectrometry analysis shows that nisin exists in a metabolite of the strain, and the strain can be used for producing nisin. The compound shows an excellent and wide antibacterial function in antibacterial experiments, can be used for preparing antibacterial drugs, particularly has a remarkable inhibiting effect on growth of gram-negative bacteria and fungi, can be used for preparing drugs for relieving or treating enteritis, or preparing drugs, nourishment or health care products for regulating gastrointestinal tract micro-ecological balance of people or animals, and can be used for preparing medicines for relieving or treating enteritis. The compound can also be used for preparing antibacterial food additives and / or animal feed additives. A hemolysis experiment result shows that hemolysis is avoided, toxic and side effects are small, and wide development prospects and application values are achieved.

The invention relates to a chemiluminescence kit for detecting pepsinogen II. The kit comprises an enzyme marker, a luminescent substrate solution and an anti-interference buffer system, the anti-interference buffer system comprises a nonionic surfactant, animal-derived IgG (immunoglobulin G), an antioxidant and catalase; the chemiluminescence kit also comprises a PG II calibration product and a PG II quality control product. The core active component of the enzyme marker is beta-galactosidase. The enzyme marker further comprises an anti-PG II monoclonal antibody marked by beta-galactosidase, bovine serum albumin, trehalose, cane sugar, MgCl2. 6H2O, sodium chloride, Proclin300 and a phosphate buffer solution. According to the kit, a brand-new anti-interference buffer system is introduced on the basis of a spatial proximity chemiluminescence analysis method, so that the resistance to clinically common interferents (such as hemolysis, lipemia, jaundice and heterotropism antibodies) is remarkably improved, and the detection result is more accurate and reliable.

The invention discloses an antibacterial peptide IFD targeting gram-negative bacteria as well as a preparation method and application thereof. The amino acid sequence of the antibacterial peptide IFD is shown as SEQ NO.1, and a C terminal is amidated by adopting-NH2. The antibacterial peptide is obtained by modifying 119-132 fragments of human-derived MD-2 protein, and is prepared by site-specific mutagenesis, hydrophobic and hydrophilic sequence insertion optimization and solid-phase chemical synthesis. The antibacterial peptide has high antibacterial activity on Gram-negative bacteria (such as escherichia coli and salmonella typhimurium), the GM Gram-negative bacteria MIC reaches 3.63 mu M and is increased by 5.24 times compared with the original peptide, the targeting property is high, and the hemolysis rate 1t when the hemolysis activity is 128 mu M is low; 3%), the Gram-negative bacteria therapeutic index is as high as 76.1, and the Gram-negative bacteria can be used as a feed antibiotic substitute for preparing drugs for treating Gram-negative bacteria infectious diseases.

The invention provides a strain of high-temperature-resistant and inflammation-relieving Witzia ciliaris and an application thereof, the high-temperature-resistant and inflammation-relieving Witzia ciliaris is Witzia ciliaris CN985 and is preserved in the China Center for Type Culture Collection (CCTCC), the preservation date is April 7, 2024, and the preservation number is CCTCC NO: M 2024641. The invention further provides a preparation method of the high-temperature-resistant and inflammation-relieving Witzia ciliaris strain for preventing and treating inflammation of the high-temperature-resistant and inflammation-relieving Witzia ciliaris strain for preventing and treating inflammation of the high-temperature-resistant and inflammation-relieving Witzia ciliaris strain. The invention provides a brand new strain of Witzia ciliaris CN985, which is resistant to high temperature, has better acid resistance and bile resistance, and has no hemolysis risk when being applied to a human body. The Witzia coagulans CN985 has excellent antibacterial performance, and especially can significantly inhibit streptococcus pyogenes, salmonella enteritidis and / or group B streptococcus; besides, experimental results show that the Witzia ciliaris CN985 can relieve enteritis, improve the immunity of the organism and relieve pharyngitis, and especially when the Witzia ciliaris CN985 and the phytobacterium plantarum CE18 are combined for use, the Witzia ciliaris CN985 and the phytobacterium plantarum CE18 have a synergistic interaction effect, so that the relieving effect on enteritis can be remarkably improved.

An implantable pump system is provided, including an implantable blood pump suitable for use as a partial support assist device, the system further including an extracorporeal battery and a controller coupled to the implantable pump, and a programmer selectively periodically coupled to the controller to configure and adjust operating parameters of the implantable pump. The implantable pump includes a flexible membrane coupled to an electromagnetic actuator including a magnetic assembly and electromagnetic assembly, so that when the electromagnetic assembly is energized, the electromagnetic assembly causes wavelike undulations to propagate along the flexible membrane to propel blood through the implantable pump. The controller may be programmed by a programmer to operate at frequencies and duty cycles that mimic physiologic flow rates and pulsatility while operating in an efficient manner that avoids thrombus formation, hemolysis and / or platelet activation.

The invention discloses an antibacterial peptide PA targeting multi-drug-resistant gram-negative bacteria and application of the antibacterial peptide PA, and the amino acid sequence of the antibacterial peptide PA is shown as SEQ ID NO.1. The antibacterial peptide PA provided by the invention has a remarkable antibacterial effect of the multi-drug-resistant gram-negative bacteria, is quick in bactericidal effect, does not have hemolysis and cytotoxicity, does not cause renal toxicity after treatment, and can be used for preparing the antibacterial peptide PA for treating the multi-drug-resistant gram-negative bacteria. The biological safety is good, and the compound can be used for treating bacterial infection and can also be applied to other scenes needing sterilization or bacterial growth inhibition.

The invention belongs to the technical field of gene engineering, and particularly relates to ALFPm3 modified antibacterial peptide, a molecular design and construction method and application of the ALFPm3 modified antibacterial peptide, and the amino acid sequence of the ALFPm3 modified antibacterial peptide is selected from any one of SEQ ID NO: 1-SEQ ID NO: 7. Analysis and design are carried out by combining the structure and action site of vibrio parahaemolyticus and the molecular structure and function relationship of the antibacterial peptide ALFPm3 as a basic template, the antibacterial activity of the antibacterial peptide ALFPm3 is improved, and other biological activities such as hemolysis are reduced.

This invention discloses an antimicrobial peptide PA targeting multidrug-resistant Gram-negative bacteria and its application. The amino acid sequence of the antimicrobial peptide PA is shown in SEQ ID NO.1. The antimicrobial peptide PA provided by this invention has a significant antibacterial effect against multidrug-resistant Gram-negative bacteria, and has a rapid bactericidal onset, no hemolysis or cytotoxicity, does not cause nephrotoxicity after treatment, and has good biosafety. It can be used for the treatment of bacterial infections and can also be applied to other scenarios that require bactericidal or antibacterial growth inhibition.

The application discloses a hemolysis analyzer based on an integrated optical waveguide sensor and a preparation method thereof, and comprises an integrated photon waveguide, a nano filter and a micro-fluidic channel, wherein the integrated photon waveguide is a single waveguide with a middle spiral type and coupling gratings on both sides. The hemolysis analyzer fully utilizes the advantages of high sensitivity and small volume of the integrated optical waveguide sensor, and realizes sample-free preparation by combining the methods of instantaneous absorption and hemoglobin filtration, and can quickly and accurately detect hemolysis and the degree of hemolysis.

The invention discloses a red blood cell room-temperature preservation solution as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The erythrocyte preservative comprises the following components in parts by weight: 1-200 parts of an anticoagulant, 1-200 parts of a blood cell stabilizer, 1-100 parts of a metabolic inhibitor, 1-100 parts of an electrolyte, 1-150 parts of an antioxidant and 350-1000 parts of deionized water, and the pH value of the erythrocyte preservative is 4.6-7.0. All the components in the preservative are mutually matched and have a synergistic effect, and the integrity of erythrocyte membranes can be kept through synergistic protection of the multiple components; the electrolyte equilibrium osmotic pressure is maintained, the antioxidant scavenges free radicals, and hemolysis is reduced; the preservation time is prolonged, and the cell morphology and function are good; the dependence on a cold chain is reduced, and the demand on deep hypothermia equipment is reduced.

The application belongs to the field of polypeptides, and particularly relates to a marine-derived antibacterial peptide, a mutant thereof, an antibacterial composition and application. The application screens a marine-derived antibacterial peptide, and optimizes net charge, hydrophobicity and amphiphilicity of the antibacterial peptide through mutation. Through experimental testing, the four antibacterial peptides have differential antibacterial effects on different gram-negative bacteria, wherein the antibacterial peptide mutant shown in SEQ ID NO. 2 has the best antibacterial effect and low hemolysis, and has a positive application prospect.

The invention relates to the technical field of biological pharmacy, and particularly discloses application of antibacterial small molecule peptide in preparation of antibacterial infection drugs. The antibacterial small molecule peptide is VeAMP, and the amino acid sequence of the antibacterial small molecule peptide is MKIVKRILLVL-NH2. The small molecule peptide VeAMP provided by the invention shows remarkable antibacterial activity on pan-drug-resistant acinetobacter baumannii, has the advantages of rapid sterilization, low hemolysis, low cytotoxicity and good in-vivo safety, is not easy to induce bacterial drug resistance after being exposed for a long time at a sub-inhibitory concentration, can effectively reduce tissue bacterial load, remarkably improves the survival rate of infected mice, and has a good application prospect. The invention provides a novel and safe candidate antibacterial agent which is not easy to generate drug resistance for preventing and treating drug-resistant acinetobacter baumannii infection.

The invention provides a carotid artery thrombus treatment catheter and a suction system comprising the same, and belongs to the technical field of medical instruments. The treatment catheter comprises a catheter body, a blocking balloon, a balloon pressurizing cavity and a Luer connector. The suction system comprises a Y-shaped connector, a blood transfusion module and a pressure monitoring system. Gapless wall attachment of the blood vessel wall is achieved through the blocking balloon, thrombus residues are avoided, suction-instrument conveying integrated operation is achieved in cooperation with the Y-shaped connector, and the multi-instrument exchange process is simplified; the balloon pressurizing cavity can accurately control the plugging balloon to expand to block blood flow, and thrombus fragments are prevented from being migrated; the peristaltic pump and the filter assembly of the blood transfusion module are connected through the catheter inner cavity, thrombus is sucked, filtered blood is transfused back, blood loss is reduced, the hemolysis risk is reduced, and the technical problems of thrombus residues, large blood loss amount, tedious operation, insufficient safety and the like are solved. The thrombus is efficiently removed, blood loss is remarkably reduced, the process is simplified, and the treatment safety is guaranteed through pressure monitoring.

Aspects of the present disclosure provide anti-TMPRSS6 antibodies and methods of using the same to promote hepcidin expression and treat iron overload related disorders such as hemopigmentation, sickle cell disease, thalassemia, hemolysis, Diamond-Blackfan anemia, myelodysplastic syndrome (MDS), and blood transfusion.

A catheter extension set includes a distal end including a luer adapter configured to couple to a catheter adapter, a proximal end including a blood collection device, and an extension tube extending between the distal end and the proximal end. The catheter extension set can increase flow resistance, which can reduce the risk of hemolysis when there is a high pressure differential within the catheter extension set. The catheter extension set can decrease flow resistance after the pressure differential within the catheter extension set is reduced, which can facilitate faster blood collection.

The application belongs to the technical field of antibacterial medicine, and particularly relates to a cinnamaldehyde-modified polypyridine ruthenium complex for inhibiting hemolysis, a preparation method and application thereof. The cinnamaldehyde-modified polypyridine ruthenium complex has a structure as shown in formula I. 1 , formula I 2 or formula I 3 The cinnamaldehyde-modified polypyridine ruthenium complex can effectively reduce the hemolysis of red blood cells caused by toxins released by Staphylococcus aureus, and the ruthenium complex does not induce the drug resistance of bacteria, and can significantly increase the sensitivity of Staphylococcus aureus to aminoglycoside antibiotics.

The present application relates to a kind of single fatty chain choline phospholipid and its preparation method and application, belong to the field of biomedical materials.The single fatty chain choline phospholipid of the present application, structural formula is as shown in formula (I) or formula (II).Single fatty chain choline phospholipid can be applied in preparation of the medicament for reducing the content of lysophospholipid in human body.The single fatty chain choline phospholipid of the present application can produce strong charge interaction with lysophospholipid, and then combine with lysophospholipid in vivo, so that lysophospholipid loses lysolabine toxicity, weaken the hemolysis harm caused to red blood cell due to high concentration of lysophospholipid in vivo, thereby reduce the harm of lysophospholipid to liver disease patient lacking lysophospholipase.

The application discloses a biomedical test strip detection data analysis method and system based on multiple algorithms. The method comprises the following steps: obtaining related data of a test paper to be detected, wherein the related data comprises T-line related data, B-line related data and C-line related data; verifying whether the lengths of three curves corresponding to the related data are consistent, if not, determining that the detection result is invalid, if yes, analyzing sample adding characteristics to determine whether there is sample adding sequence error or no sample adding; distinguishing sample types according to chromatography speed according to the related data; when the sample type is a whole blood type, calculating the length of a sample type identification point corresponding to three lines, adjusting and cutting off, and if the total length exceeds a set threshold, determining that a hemolysis phenomenon leads to an invalid detection result. The method of the application particularly focuses on processing the hemolysis phenomenon and other user operation related problems, thereby enhancing the reliability and efficiency of the overall detection system.

The application discloses a sample information identification method, a computer readable storage medium and an identification device. The sample information identification method comprises the following steps: transporting a sample tube to a first identification area by controlling a transportation guide rail, acquiring sample tube information by a sample tube information reading mechanism; controlling an identification mechanism to capture a first image information of the sample tube; analyzing the first image information to acquire first identification information; comparing the first identification information with the sample tube information; if the first identification information matches the sample tube information, adjusting the position of a mechanical hand according to the first identification information to clamp the sample tube and move the sample tube to a second identification area; and acquiring sample information of the sample tube located in the second identification area. According to the sample information, whether the blood sample in the sample tube has hemolysis, jaundice and chyle phenomena and the severity information of the disease can be obtained, so that an abnormal sample with a relatively large influence on detection error can be identified.

A tangent flow hemolysis blood testing assembly, device and method are described herein.

The utility model provides a kind of blood specimen transmission cylinder, including main body cylinder, first buffer sleeve and cylinder cover;Wherein main body cylinder has accommodating cavity;First buffer sleeve is installed in accommodating cavity, blood collection tube can be inserted into first buffer sleeve;Cylinder cover can be connected with main body cylinder cover, after cover connection, the inner top wall of cylinder cover and the tube cap of blood collection tube abut.In transmission blood specimen, blood collection tube with blood specimen is placed in first buffer sleeve, then cylinder cover is connected with main body cylinder cover and fixed blood collection tube.The blood specimen transmission cylinder of the utility model can effectively avoid specimen spilling and hemolysis problem in transmission process, specifically, blood collection tube is placed in first buffer sleeve, the impact of transport vibration on blood collection tube is reduced using the buffer effect of sleeve, and the stability of tube body is enhanced, so as to prevent hemolysis;In addition, cylinder cover and main body cylinder are reliably fixed by clamping structure, and the pre-tightening pressure exerted on blood collection tube cap by cylinder cover inner wall can prevent tube cap from loosening and avoid spilling.

The invention discloses a magnetofluid-driven pump-free blood purification system, and belongs to the technical field of medical apparatuses and instruments. The system breaks through a traditional mechanical pump driving mode, magnetic nanoparticles are introduced into blood, the blood is driven to flow by means of magnetic force generated by a gradient magnetic field, and a pump-free blood purification system is constructed. Compared with a traditional blood purification system, the blood purification system has the advantages that damage of high shear force generated by a mechanical pump to blood cells is thoroughly avoided, and the hemolysis risk is effectively eliminated; continuous operation of a mechanical pump is not needed, and energy consumption is remarkably reduced; no mechanical part runs, so that the noise is greatly reduced; meanwhile, huge structures of a mechanical pump and a driving system thereof are eliminated, and miniaturization of equipment is facilitated. The system provides a brand new thought for blood purification treatment, has safety, high efficiency and convenience, is expected to bring a new change to the medical field, and meets the blood purification requirements in different scenes.

A method of characterizing a specimen to be analyzed in an automated diagnostic analysis system provides a segmentation determination and / or an HILN (hemolysis, icterus, lipemia, normal) determination of the specimen while providing characterization training updates based on the accuracy and / or confidence in the determinations. The method includes identifying an incorrect or low confidence segmentation or HILN determination, forwarding the incorrect or low confidence determination from the HILN network to a database, and providing one or more training images to the HILN network based on the incorrect or low confidence determination. Quality check modules and systems configured to carry out the method are also described, as are other aspects.