1131results about How to "High expression" patented technology

The invention relates to a high-activity recombinant XVII type collagen as well as a rapid preparation method and application thereof, and belongs to the technical field of bioengineering. The amino acid sequence of the high-activity recombinant XVII type collagen is as shown in SEQ ID NO. 1, and the nucleotide sequence of the high-activity recombinant XVII type collagen is as shown in SEQ ID NO. 2. The invention also provides a preparation method and application of the recombinant XVII type collagen. The high-activity recombinant XVII type collagen has good biological activity, has good effects of promoting cell adhesion, cell proliferation and cell migration, and can be compounded with other proteins to form raw materials of tissue engineering products, cosmetics, health care products or medicines. The high-activity recombinant XVII type collagen gene is integrated to different positions of a yeast genome through three different vectors, and the copy number of a target gene is increased, so that the expression quantity of the recombinant XVII type collagen is remarkably increased, and the yield of the high-activity recombinant XVII type collagen is effectively increased.

The invention discloses a remote sensing image ground object segmentation method based on hierarchical feature extraction and decoding, which relates to the technical field of remote sensing image processing, and comprises the following steps: step 1, collecting and preprocessing a remote sensing image, and constructing a training sample set; 2, constructing an image segmentation network, wherein the image segmentation network comprises a hierarchical encoder, a receptive field module unit and a network decoder which are connected in sequence; 3, training and optimizing the image segmentation network by adopting the training sample set to obtain a remote sensing image ground object segmentation model; and 4, collecting a remote sensing image to be segmented, and inputting the remote sensing image to the remote sensing image ground object segmentation model to obtain a ground object segmentation result. According to the method, the advantages of the advanced visual basic model and the U-shaped network architecture are combined, so that rapid and accurate segmentation of the ground target is realized.

The invention discloses a human face sensitive data protection method based on an elastic anti-interference privacy protection mechanism, belongs to the field of information processing, and provides a dynamic self-adaptive sampling strategy based on information entropy in a data preprocessing stage, and the sampling rate is adjusted according to the distribution characteristics of a human face data set and the weight of sensitive information. And the model performance is maximized on the premise of ensuring user privacy. In order to obtain a better privacy protection effect, an entropy adjustment differential privacy mechanism is designed, and Gaussian noise of different degrees is adaptively added according to an information entropy value of face data after a face data set is dynamically and adaptively sampled, so that refined privacy protection of face sensitive data under differential privacy constraint is realized. In a model training stage, an elastic anti-interference training method is provided, diversified adversarial samples are dynamically generated in different stages of model training, and the over-fitting risk of the model is reduced while the robustness of the model to various types of adversarial attacks is enhanced.

The invention provides an influence maximization method and system based on deep reinforcement learning and a graph neural network, and belongs to the field of influence maximization in social network analysis. The method aims to optimize selection of seed nodes through a reinforcement learning technology so as to realize maximum propagation of information in a network, and the graph neural network can accurately capture an interaction relationship between nodes in a graph structure by virtue of a message transmission mechanism of the graph neural network, which is highly matched with an information propagation process in a cascade propagation model. Therefore, in the influence maximization model based on the deep reinforcement learning and the graph neural network, the graph neural network is combined with the deep reinforcement learning, the embedded representations of the nodes are learned by using the graph neural network, and the embedded representations are used as the input of the deep Q network so as to fully capture the complex structure and node relationship in the network. In addition, a multi-head attention mechanism is introduced to aggregate information of each round, expression of key features is enhanced, and information redundancy is effectively reduced.

The invention belongs to the technical field of bioengineering, and particularly relates to recombinant III-type humanized collagen as well as a coding gene and application thereof. According to the invention, a novel III-type humanized collagen sequence is designed by adopting an advanced AI technology and molecular dynamics simulation, so that the recombinant III-type humanized collagen product is ensured to have high stability and degradation resistance. According to the invention, the screened new III-type humanized collagen sequence is transferred to the pichia pastoris strain for recombinant expression, and the prepared recombinant III-type humanized collagen has the advantages of strong stability, high expression quantity and good hydrophilicity, and meanwhile, the recombinant III-type humanized collagen also has obvious effects of promoting cell proliferation and cell adhesion.

The invention relates to the technical field of parallel robot control, in particular to a three-degree-of-freedom parallel robot control parameter adjusting method and system. The method comprises the following steps: carrying out end operation space analysis on the parallel robot, and carrying out work pose space optimization to obtain optimized operation space data; performing high-speed motion residual vibration feature extraction on the parallel robot by optimizing operation space data, and performing control parameter intelligent adjustment strategy processing to obtain an intelligent vibration control parameter adjustment strategy; performing real-time vibration monitoring on an end effector of the parallel robot to generate real-time end vibration characteristic data; and intelligent feedforward compensation control adjustment processing is performed on the real-time tail end vibration characteristic data through an intelligent vibration control parameter adjustment strategy, so that intelligent parameter adjustment control operation of the three-degree-of-freedom parallel robot is realized. Through intelligent vibration control parameter adjustment, the residual vibration of the parallel robot after high-speed movement is effectively inhibited, and the working efficiency is improved.

The invention provides lactobacillus reuteri HNU872 and application of the lactobacillus reuteri HNU872 in preparation of intestinal inflammation related products. The invention discloses how the Lactobacillus reuteri HNU872 effectively alleviates the phenotype of DSS-induced mouse enteritis. The invention also discloses a preparation method of the Lactobacillus reuteri HNU872. Research results show that the Lactobacillus reuteri HNU872 can significantly relieve weight loss caused by enteritis, improve colon length, significantly improve the level of anti-inflammatory cytokines in serum and reduce the concentration of pro-inflammatory cytokines. In addition, the expression of the intestinal tight junction protein ZO-1 is obviously promoted. The lactobacillus reuteri HNU872 can regulate diversity and structure of intestinal microbiota of mice, inhibit enrichment of pathogenic bacteria and relieve inflammatory response. According to the invention, the action mechanism of the lactobacillus reuteri HNU872 in improving enteritis is clarified, and a solid scientific basis is provided for developing a microbial product based on probiotics as an alternative treatment strategy for ulcerative colitis.

The invention discloses an RNA (Ribonucleic Acid) in-vitro cyclization and rolling circle translation method based on an I-type intron and an application of the RNA in-vitro cyclization and rolling circle translation method. According to the invention, a ribonucleic acid construct is obtained by splitting a specific non-complementary region of the I-type intron, and the preparation of'scarless' (traceless) circular RNA is realized through a ribozyme self-splicing principle and can be popularized to other I-type intron. By optimizing a splitting structure, high-efficiency cyclization can be realized without additionally adding GTP for catalysis under the condition of no homologous arm. Meanwhile, the original part of the ribonucleic acid construct is optimized, so that the cyclization efficiency is further improved. Particularly, a rolling circle translation (RCT) technical platform based on the CITE sequence is established, and the expression quantity of the protein or the polypeptide is greatly improved. Through verification, the cyclization and rolling circle translation method provided by the invention shows potential in vaccine and CAR treatment, and is expected to be used for clinical treatment of various diseases.

The invention provides a porcine epidemic diarrhea virus S protein mutant, a nucleic acid molecule, a subunit vaccine and application. Compared with wild type porcine epidemic diarrhea virus S protein, the porcine epidemic diarrhea virus S protein mutant has the advantage that amino acid at the 1076th site, amino acid at the 1077th site, amino acid at the 1016th site and amino acid at the 1017th site are mutated into proline. The natural trimer which is stable and maintains the conformation of the S protein before fusion is constructed, the safe and efficient PEDV subunit vaccine is obtained, and the use effect and immunogenicity of the porcine epidemic diarrhea vaccine can be improved; moreover, compared with a wild type S protein, the porcine epidemic diarrhea virus S protein mutant disclosed by the invention has a higher expression quantity.

The invention relates to the technical field of recombinant protein expression, in particular to recombinant human-derived III-type collagen as well as a preparation method and application thereof. The invention provides a recombinant human source III type collagen. The amino acid sequence of the recombinant human source III type collagen is shown as SEQ ID NO. 1. The recombinant human-derived III-type collagen prepared by the invention can be better applied to a human body, better exerts biological functions of the collagen, avoids repulsive interaction, is high in safety, can effectively promote cell proliferation and oxidation resistance, and is high in expression yield, stable in batches and good in quality; the recombinant human III type collagen prepared by the invention has the potential of being applied to preparation and production of biological medicines and cosmetics.

The invention relates to an X-ray image contraband detection method based on multi-scale feature fusion, and belongs to the technical field of image processing. The method comprises the following steps: acquiring an X-ray contraband data set, and dividing the data set into a training set and a test set; constructing an X-ray image contraband target detection model based on multi-scale feature fusion, wherein the model comprises an image branch, a self-adaptive high-low pass filter module, a text branch, a Neck layer and a Head head; images in the training set are input into the model to train the model; optimizing the model by adopting a loss function to obtain a trained model; and inputting the images in the test set into the trained model to obtain a contraband detection result. According to the method, through image and text joint training, the performance is improved in real-time task monitoring, the efficiency is higher, the calculation amount and memory occupation are reduced, and the problem that image boundary details are fuzzy in a complex scene is solved.

The invention discloses a liver-targeting drug liposome and application thereof, and relates to the field of drug delivery, cholate and mannose are used for respectively modifying phospholipid molecules DSPE-PEG2000, two raw materials DSPE-PEG2000-CA and DSPE-PEG2000-MAN composed of the liposome are synthesized, and then the liposome is prepared into the liver-targeting drug liposome. The liposome with liver targeting property is obtained from the liposome, cholesterol, DSPC, DC-cholesterol and a water-soluble therapeutic drug according to a certain proportion, the liposome has relatively high cell safety and liver targeting property, and the therapeutic effect of the corresponding drug can be remarkably improved after the drug is embedded.

The invention provides an application of a turnip crinkling virus P8 in inhibiting RNA silencing, and the nucleotide sequence of the P8 gene is as shown in SEQ ID NO: 1. Researches find that P8 has a silent inhibition function and can inhibit mRNA (messenger ribonucleic acid) of GFP (green fluorescent protein) from being degraded, so that the expression quantity of GFP protein is increased; in addition, it is found that the P8 can also increase the number of particles of an arabidopsis thaliana mRNA degradation pathway Procness body (a compound formed by interaction of Dcp1 protein and Dcp2 protein), and research results lay a foundation for clarification of P8 functions.

The invention provides a recombinant XVII type collagen with a triple helix structure, and belongs to the technical field of biological proteins. The amino acid sequence of the collagen comprises one or more amino acid residue repetitive units, each repetitive unit comprises a sequence as shown in SEQ ID NO.1 or SEQ ID NO.2, the number of repetitions is n, and n is larger than 1 and smaller than or equal to 60. The recombinant XVII type collagen provided by the invention is simple in production process, relatively high in expression quantity, low in immunogenicity, high in safety, relatively good in stability of the triple-helix structure, and strong in supporting capacity for skin cells; efficacy experiments show that the collagen has higher cell adhesion, wound healing promotion and hair follicle repair promotion capability than XVII type collagen products in the market at present, and can be widely applied to the fields of food, health care products, cosmetics, medical instruments and the like.

The invention belongs to the technical field of bioengineering, and particularly relates to recombinant III-type humanized collagen and application thereof. Area fragments with good hydrophilicity in a human III-type collagen alpha chain are selected and sequentially connected to serve as expression protein, the amino acid sequence is 100% identical to that of a corresponding part of natural human III-type collagen, and immunological rejection cannot be generated when the human III-type collagen is applied to a human body. According to the invention, the screened new III-type humanized collagen sequence is transferred into pichia pastoris for recombinant expression, the prepared recombinant III-type humanized collagen has the advantages of strong stability, high expression quantity and good hydrophilicity, and meanwhile, the recombinant III-type humanized collagen also has obvious effects of promoting cell proliferation and cell adhesion.

The invention relates to a method for improving luminous intensity of a plant by introducing an exogenous caffeic acid synthesis pathway. A TAL gene and a P450 gene are introduced into a plant body to obtain a remarkable effect of increasing the expression quantity of caffeic acid. Due to the fact that introduction of the TAL gene and the P450 gene promotes deamination of the L-DOPA, the accumulation amount of the L-DOPA is increased, and then the expression amount of caffeic acid in the plant is effectively increased. Based on the pathway, the synthesis of fluorescein in the plant is increased, so that the luminous intensity of the plant is enhanced.

The invention relates to the technical field of biology, in particular to application of N-terminal truncated 8-isopentenyl transferase in production of 8-isopentene kaempferol. The invention provides the N-terminal truncated 8-isopentenyl transferase, and the N-terminal truncated 8-isopentenyl transferase can be used for carrying out isopentenylation on kaempferol to generate 8-isopentene kaempferol. Specifically, the saccharomyces cerevisiae is used as a chassis, under the condition that kaempferol is exogenously added, through overexpression of 8-isopentenyl transferase, biosynthesis of 8-isopentenyl kaempferol in the saccharomyces cerevisiae is realized, and meanwhile, a foundation is laid for subsequent biosynthesis of icaritin. The invention provides a novel reference scheme for biosynthesis of flavonoid compounds.

The invention discloses an OpenCRISPR (Open Clustered Regularly Interspaced Short Palindromic Repeats)-NLS (Non-Least Squares) fusion protein for gene editing of penaeus monodon, a construction method of the OpenCRISPR-NLS fusion protein and a nucleotide construct containing the OpenCRISPR The invention also discloses an application of the fusion protein or the nucleotide construct in gene editing of penaeus monodon and an application of the fusion protein or the nucleotide construct in population improvement of aquaculture penaeus monodon. According to the invention, codon optimization is carried out on a coding sequence of OpenCRISPR-1 protein, and a nuclear localization signal (NLS) is fused with the OpenCRISPR protein subjected to codon optimization; the method has the advantages of being efficient in gene expression, accurate in targeting positioning capacity, excellent in genome editing efficiency, capable of reducing the off-target effect, wide in adaptability, capable of promoting sustainable aquaculture, remarkable in economic and social value and the like.

The invention discloses a multi-target fusion dairy cow oestrus behavior detection method and system, and belongs to the technical field of target detection of deep learning. The method comprises the following steps: S1, collecting a dairy cow oestrus behavior image, and performing image enhancement on the dairy cow oestrus behavior image; s2, constructing a PBF-YOLO deep learning network model, wherein the PBF-YOLO deep learning network model comprises a lightweight module, a background information enhancement module and a feature aggregation module; and S3, identifying a dairy cow oestrus behavior region of interest through the PBF-YOLO deep learning network model, and detecting the dairy cow oestrus behavior. The invention provides a solution for the situation that the existing lightweight algorithm is low in detection precision and poor in robustness due to the fact that the cow oestrus behavior and other background features are similar and are difficult to distinguish in an actual pasture and cows with different scales appear on the same image.

The invention relates to the technical field of gene engineering, in particular to nucleic acid for encoding kreotoxin and a preparation method of the kreotoxin. The invention provides nucleic acid for encoding the botulinum toxin, the nucleic acid is subjected to codon optimization, and the scheme is proved to be superior to other optimized sequences. Through verification, the sequence has a relatively high expression quantity in escherichia coli and is in soluble expression, and the prepared kreotoxin has good activity.

The invention belongs to the technical field of bioengineering, and provides a recombinant XVII type collagen as well as a preparation method and application thereof. A target gene sequence is obtained through bioinformatics predictive analysis and integrated design on the basis of a human XVII type collagen sequence, codon optimization is carried out, a T7 promoter in a plasmid vector is optimized, an engineering strain of the high-hydrophobicity humanized XVII type collagen is prepared, and the high-hydrophobicity humanized XVII type collagen is obtained. The strain is subjected to culture and inducible expression to obtain high-yield recombinant XVII type collagen. The recombinant XVII type collagen disclosed by the invention is reasonable in sequence design, large in expression quantity, simple in preparation method and stable in heredity, and rapid separation of the collagen can be realized in a production and preparation process by utilizing high hydrophobicity.

The invention relates to the technical field of microorganisms, in particular to recombinant pichia pastoris for expressing plastic degrading enzyme LCCICCG as well as a construction method and application of the recombinant pichia pastoris. According to the invention, pichia pastoris is taken as a host cell, the coding gene lccICCG of the plastic degrading enzyme is heterologously expressed, and single-copy or multi-copy recombinant pichia pastoris is constructed. By optimizing culture conditions, the expression quantity and biomass of the plastic degrading enzyme protein are remarkably improved; by optimizing a fermentation strategy, high-density fermentation production of the LCCICCG protein in a 5L fermentation tank is realized, and a technical basis is provided for realizing large-scale industrial production of the protein. The improvements not only improve the production efficiency and reduce the cost of industrial production, but also provide powerful support for commercial application of the plastic depolymerases.

The invention discloses application of a Wnt2 gene in buffalo ovarian granular cells, and belongs to the technical field of cell engineering and gene engineering. Wnt2 is taken as a research object, the Wnt2 overexpression vector or Wnt2 siRNA is transfected to the ovarian granulosa cells respectively by constructing the Wnt2 overexpression vector and synthesizing small interfering RNA (Wnt2 siRNA), then changes of signal channel gene mRNA and protein level related to ovarian granulosa cell apoptosis, proliferation and E2 and P4 secretion are detected, and finally phenotypic changes of the ovarian granulosa cells are detected. Results show that Wnt2 can inhibit apoptosis of ovarian granular cells and promote proliferation and E2 and P4 secretion. By exploring the application of the Wnt2 in the ovarian granular cells, the application has good application in researching an ovarian follicle atresia mechanism, initial estrus initiation and the like.

The invention discloses a polygonatum cyrtonema transcription factor gene PcWRKY1 as well as an encoding protein and application thereof. According to the application, a WRKY transcription factor PcWRKY1 which is obviously inhibited by high-temperature stress is screened, the transcription level of the PcWRKY1 transcription factor gene responding to the high-temperature stress is further determined, a tissue expression mode and subcellular sites of the PcWRKY1 transcription factor gene are analyzed, and finally, the regulation effect of the PcWRKY1 transcription factor gene on the high-temperature tolerance of the polygonatum cyrtonema protoplast is determined. The PcWRKY1 transcription factor gene is located in a cell nucleus, codes a protein containing 519 amino acids and is expressed in roots, stems, leaves, tubers and seeds of polygonatum cyrtonema, the expression quantity in the seeds is the highest, and the PcWRKY1 transcription factor gene can inhibit the increase of MDA content and promote the improvement of CAT enzyme activity under high temperature stress. Further, the tolerance of the polygonatum cyrtonema protoplast under high-temperature stress is improved.

The invention provides a recombinant engineering bacterium for preparing recombinant albumin based on a transcription factor and application, and relates to the technical field of gene engineering. According to the present invention, while the recombinant albumin is expressed, the SUS1 gene and the SUA7 gene are simultaneously overexpressed and integrated into the strain, and / or one or more genes of YHP1 and HDA1 are subjected to targeted knockout by using the CRISPR-Cas9 gene editing technology so as to improve the expression quantity of the recombinant albumin; according to the recombinant engineering bacterium, the yield of recombinant albumin can be remarkably increased, and the recombinant engineering bacterium has a great application value.

The invention discloses a site-specific integration site screening method, a carrier particle, a site-specific integration method and a particle. The invention provides a site-specific integration site screening method, a carrier particle, a site-specific integration method and a particle, and aims to integrate upstream and downstream marker genes with recognition sequences on the carrier particle, perform protein expression quantity screening while performing monoclonal screening, obtain a monoclonal carrier with good performance through one-step screening, and perform site-specific integration on the carrier particle. The marker gene is replaced when the exogenous target gene is expressed, so that the overall stability of the particle is greatly ensured, the overall expression performance and stability of the expression particle are ensured while the development of the expression particle is shortened, and the technical problem that an expression vector in the prior art is relatively long in development time or poor in performance is solved. The site-specific integration site screening method provided by the invention can effectively shorten the development period of the expression vector with high expression quantity and overall stability. The carrier particle provided by the invention is good in adaptability and good in expression quantity and passage stability.

The invention discloses a method for modifying lutein lipidosome by composite polyphenol, which comprises the following specific steps: (1) dissolving lutein and a lipid material to obtain a lutein lipid solution, and carrying out rotary evaporation to form a film; (2) dissolving the composite polyphenol in a thermal phosphate solution, and hydrating the xanthophyll film; (3) carrying out rotary evaporation after hydration to form a film, and adding a cholate solution for redissolving; and (4) after ultrasonic treatment, passing through a polycarbonate film and extruding to obtain the lutein liposome. The lutein liposome modified by the composite polyphenol has the characteristics of high stability and high bioavailability, and the accumulation of lutein in liver and plasma can be remarkably improved.

The invention provides a group photo interaction method and device based on a virtual image, electronic equipment, a computer readable storage medium and a computer program product. The method comprises the steps that a target group photo template comprising a first bearing position and at least one second bearing position is displayed, the first bearing position bears a first virtual image corresponding to a current object, and the second bearing position is used for bearing a virtual image corresponding to an invited interaction object; in response to an information editing operation triggered based on the target group photo template, displaying edited impression information for the interaction object; in response to a group photo invitation operation for the interaction object, sending a group photo invitation carrying the impression information to a terminal corresponding to the interaction object; and when the interaction object responds to the group photo invitation based on the impression information, displaying a second virtual image at a second bearing position in the target group photo template to obtain a target group photo comprising the first virtual image and the second virtual image. According to the invention, the group photo interactivity based on the virtual image can be improved.

The invention relates to a mutant of an RSV pre-fusion conformation F protein, the mutant comprises amino acid mutation relative to an amino acid sequence of a wild type RSV F protein, the amino acid mutation is that amino acids at positions 392 and 493 or near the 493 are substituted by C, and a disulfide bond is formed between mutated positions 392C and 493C or near the 493C. The invention also provides a related DNA (deoxyribonucleic acid) coding sequence, a recombinant plasmid, an mRNA (messenger ribonucleic acid) sequence and a corresponding RSV vaccine. By introducing disulfide bonds at proper positions and combining with cavity filling, electrostatic mutation, proline mutation and the like, the expression and stability of the F protein with conformations before and after fusion and trimerization conformations are remarkably improved, and the F protein is remarkably inhibited from being converted into conformations after fusion. The mutant is also applied to preparation of RSV vaccines and detection of respiratory syncytial virus infection.

The invention relates to the field of bioengineering, in particular to application of integration sites as targets in production and / or preparation of icaritin, and the integration sites comprise one or more of a YPRC [delta] 15 site, a YORW [delta] 22 site, a HO site, a YNRC [delta] 9 site, a PDC5 site, a YCRW [delta] 12 site and a YMRW [delta] 15 site. The saccharomyces cerevisiae is used as a chassis cell, two-step conversion from kaempferol to icaritin is focused on, a novel method for enhancing synthesis of icaritin from the perspective of pathway engineering and enzyme engineering is provided, and an advanced strategy is provided for biosynthesis of flavonoid compounds and natural products.