158results about How to "High expression" patented technology

This invention provides novel stem cell activators, topical skin agents containing the aforementioned stem cell activators, and skin cosmetics containing the aforementioned stem cell activators. The stem cell activator of this invention comprises at least one compound selected from compounds (including optically active agents) shown in formula (1), salts of the aforementioned compounds, and hydrates thereof as active ingredients. In the following formula, R... 1 R 2 Represents a hydrogen atom or a hydrocarbon group. R 3 Represents a hydrogen atom or an organic group. n represents an integer greater than or equal to 1. The above hydrocarbon groups may have substituents. [Chemical Formula 1]

The present application relates to the technical field of biology, and discloses a construction method of a Pseudomonas putida suicide vector and application thereof.The construction method of the Pseudomonas putida suicide vector provided by the present application takes a pK18mobsacB vector as a template to obtain a vector skeleton by amplification, and then takes Pseudomonas putida KT2440 bacterial liquid as a template to obtain a strong promoter Prib, upper and lower homologous arms of a gene x to be knocked out, and connects the upper and lower homologous arms to the vector skeleton to obtain the Pseudomonas putida suicide vector.The strong promoter Prib can increase the expression amount of sacB sucrose in the Pseudomonas putida, and enhance the sucrose lethal effect.The Pseudomonas putida suicide vector obtained can be introduced into the Pseudomonas putida, and then the strain with the target gene to be knocked out deleted can be obtained efficiently and accurately through kanamycin positive screening and sucrose negative screening.

The application discloses a bacteriocin synthesis regulation gene plnC overexpression and a construction method and application of a recombinant plant lactis bacillus, and has the characteristics that the nucleotide sequence is shown in SEQ ID No:1 in the sequence table, and the construction method steps are as follows: 1) taking the plant lactis bacillus genomic DNA as a template, designing specific primers and performing PCR amplification; 2) purifying and double enzyme cutting the plnC gene fragment, and connecting the linear carrier pMG36e which is also double enzyme cut to obtain a pMG36e-plnC recombination cloning carrier; (3) through an electric transformation method, the pMG36e-plnC is transferred into plant lactis bacillus B1 competent cells to obtain a plnC overexpression strain B1-plnC, and the advantages are that the synthesis of the plant lactis bacillins is promoted, the plnC gene expression amount of the overexpression strain B1-plnC is increased by more than 4.5 times, and the PlnC protein content is increased by more than 1.5 times.

This invention relates to the field of plant genetic engineering, and particularly to the application of the OsPRMT6a gene in regulating the heading time and grain weight of rice. The invention involves constructing a vector overexpressing the OsPRMT6a gene and introducing it into rice using Agrobacterium-mediated transformation. Positive transgenic plants are obtained through screening with hygromycin labeling, and further, positive plants with high expression levels are obtained. Under Nipponbare background conditions, the OsPRMT6a gene overexpression material exhibits earlier heading and increased grain weight compared to the wild type. Therefore, it is evident that overexpression of the OsPRMT6a gene described in this invention can advance rice heading and increase grain weight, possessing significant breeding value for increasing rice yield and efficiency, and reducing costs and increasing income.

Provided herein are improved rAAV (e.g., rAAV2, rAAVrh8R, etc.) for enhanced gene therapy of ocular disorders or CNS disorders wherein the rAAV comprise one or more substitutions of amino acids that interact with heparan sulfate proteoglycan. The invention provides methods for improved transduction of retinal cells and methods for treating ocular diseases with improved compositions of rAAV particles. Further provided herein are improved recombinant adeno-associated virus (rAAV) (e.g., rAAV2, rAAVrh8R, etc.) for enhanced gene therapy of disorders of the CNS. The invention provides methods for delivering the rAAV to the CNS, methods for treating disorders of the CNS with improved compositions of rAAV particles, and kits for delivering the rAAV to the CNS and / or treating a CNS disorder.

This invention provides a cold adaptation regulatory gene RAF3 / RAF6 This invention relates to the field of genetic engineering technology and its applications. Specifically, it provides a cold adaptation regulatory gene. RAF3 / RAF6 , wherein RAF3 The genome sequence is shown in SEQ ID No. 1. RAF6 The genome sequence is shown in SEQ ID No. 2. This invention also provides the... RAF3 / RAF6 The encoded protein, and its role in reducing RAF3 / RAF6 By reducing the expression level of a protein or decreasing its activity or content, the cold tolerance and photosynthetic efficiency of plants can be improved, meaning that under cold conditions, RAF3 and / or RAF6 It reduced photosynthesis while enhancing the expression of cold-response genes, ultimately inhibiting plant growth and promoting plant adaptation to cold stress.

This application relates to the field of biomedical technology, specifically disclosing a highly liquid-stable SUMO fusion influenza B NP antigen and its preparation method. The antigen is a fusion protein of a SUMO-tagged peptide and an influenza B virus nucleoprotein NP. The SUMO-tagged peptide is directly linked to the N-terminus of the influenza B virus nucleoprotein NP via peptide bonds. The antigen is dissolved in a storage buffer for liquid preservation. The preparation method includes key steps such as fusion gene construction, vector engineering construction, host transformation screening, induced expression culture, and bacterial cell disruption and purification. This antigen can be used for clinical detection of influenza B virus, preparation of quality control materials, and immunogen development. It exhibits excellent liquid preservation stability, maintaining good immunoreactivity even after long-term storage under low-temperature liquid conditions. The preparation method of this application is simple and controllable, requiring no inclusion body refolding step, and can efficiently obtain high-purity and homogeneous soluble fusion antigen.

The invention relates to the technical field of microorganisms, in particular to the technical field of agricultural microorganisms, and more particularly relates to a cellulose degradation composite flora as well as a construction method and application thereof. The cellulose degradation composite flora comprises a cellulose degradation bacterium, namely humid cellulomonas CW35 and an auxiliary strain used for digesting an intermediate metabolite of the humid cellulomonas CW35, and the auxiliary strain is one or more of pseudomonas Taiwan CW19, bacillus amyloliquefaciens L3 or microbacterium algal M266-2. Therefore, the feedback inhibition effect of a product in the cellulose degradation process of the cellulomonas can be destroyed, the expression and enzyme activity of cellulose degradation related genes are remarkably improved, and the decomposition utilization of cellulose-degrading bacteria, namely the wet cellulomonas CW35, on cellulose-containing wastes is effectively improved.

This invention relates to a traditional Chinese medicine combination for treating intrahepatic cholestasis, which is made from the following raw materials in parts by weight: Artemisia capillaris and Glycyrrhiza uralensis 5:1 and 3:1. This invention also provides the preparation of the above-mentioned medicine and its use in treating intrahepatic cholestasis. Its advantages are: the traditional Chinese medicine composition of this invention (Artemisia capillaris and Glycyrrhiza uralensis decoction) has a significant ameliorative effect on intrahepatic cholestasis (non-obstructive type) induced by a 1% CA-containing diet in mice. Artemisia capillaris and Glycyrrhiza uralensis decoction can alleviate weight loss in mice and improve liver necrosis; significantly reduce serum ALT and ALP levels in mice; reduce the content of total bile acids in the liver and decrease the proportion of toxic bile acid taurine deoxycholic acid (TDCA) in the bile acid pool; upregulate liver BSEP protein expression to promote bile acid efflux; and downregulate liver NLRP3 protein expression to alleviate liver necrosis.

The application discloses an intelligent teaching auxiliary system based on augmented reality, which combines an ORB algorithm with a single multi-task deep learning network, simultaneously realizes feature point detection, edge detection and depth estimation tasks, extracts two-dimensional coordinates of key points, edge information and three-dimensional space coordinates, ensures that the positioning of feature points is more accurate and has strong robustness, in addition, by introducing a multi-head attention mechanism, global feature maps and local feature points are weighted and fused, the expression and matching performance of feature points are optimized, the stability and accuracy of feature point detection are further improved, and reliable data input is provided for subsequent camera pose estimation; based on the extracted two-dimensional coordinates, three-dimensional coordinates and camera intrinsic parameters, combined with a PnP algorithm, the pose information of the camera is solved by minimizing the projection error, so that the virtual three-dimensional model can be accurately aligned with the real teaching target object.

The invention discloses staphylococcus warneri CCSM005 separated from human skin and capable of improving skin health and a metagen thereof, and belongs to the technical field of microorganisms and medicines. The staphylococcus warneri CCSM005 provided by the invention can be fermented to produce the L-glycine-valine dipeptide, and a fermentation supernatant of the staphylococcus warneri CCSM005 has a good effect of repairing a skin barrier function, which is specifically shown in that the activity of damaged HaCaT cells is improved in vitro, and the gene level expression of FLG, ZO-1, CLDN and Occludin can be improved at the same time after the damage. Therefore, the staphylococcus warneri CCSM005 has a huge application prospect in preparation of external daily chemical products or medicines for repairing the skin barrier.

The application discloses a prostate cancer patient pelvic floor muscle recovery evaluation system based on artificial intelligence, which comprises an original data collection module, an original data optimization module, a time sequence double-end recovery evaluation model establishment module, a recovery evaluation model performance optimization module and a whole-cycle intelligent recovery evaluation module. The application relates to the technical field of medical data processing, in particular to a prostate cancer patient pelvic floor muscle recovery evaluation system based on artificial intelligence. The scheme innovatively proposes a time sequence double-end recovery evaluation model combining an in-hospital baseline end and a home continuous time sequence end, improves the real-time performance of patient pelvic floor muscle recovery evaluation, extracts short-term time sequence recovery features by using a bidirectional long short-term memory network, extracts long-term time sequence recovery features by combining an improved convolution residual network with a parallel activation function, significantly improves the accuracy of the recovery evaluation results, and introduces an improved optimization algorithm combined with a dynamic local search strategy, thereby significantly improving the stability and accuracy of the output results of the model.

The invention discloses a small molecule binding protein design method. The method comprises the following steps: S1, a structure acquisition step: acquiring a compound structure of a target small molecule ligand and protein; s2, a candidate sequence generation step: generating a candidate protein sequence set by adopting a protein generation model and adjusting parameters corresponding to the protein generation model according to the compound structure in the step S1; s3, a primary screening step; compounding the candidate protein sequence set in the step S2 with the small molecule ligand in the step S1, predicting a compound structure I, and screening a potential sequence from the compound structure I; s4, a fine screening stage: compounding the potential sequence screened in the step S3 with the small molecule ligand in the step S1, predicting a compound structure II, and screening a preferred protein sequence I from the compound structure II. Through deep fusion of AI generation and physical screening, a layer-by-layer progressive accurate screening funnel is constructed, and the false positive rate is greatly reduced.

The invention discloses a bovine I-type tau interferon-ferritin fusion protein as well as a mutant and application thereof. The bovine I-type tau interferon is fused with a ferritin subunit, and interferon molecules are highly repeatedly and orderly displayed on the surface of a ferritin nanocage by utilizing the self-assembly characteristic of ferritin, so that a conformation suitable for the ferritin nanocage to play functions is formed, and the expression level, the structural stability and the antiviral activity of the tau interferon are remarkably improved. The fusion protein is subjected to rational design mutation to obtain a single-site or multi-site mutant with significantly improved antiviral activity and stability. According to the invention, a silkworm or insect cell eukaryotic expression system is adopted to express the fusion protein or the mutant thereof, and the expression system is safe to operate, simple and convenient in procedure, low in cost and extremely beneficial to large-scale industrial production; the prepared fusion protein or mutant nanoparticles can be applied to preparation of a plurality of drugs for preventing or treating bovine viral infection, tumors and immune system diseases and immunologic adjuvants for vaccine compatibility.

The invention relates to the technical field of bioengineering, in particular to a targeting nanoparticle for treating acute respiratory distress syndrome as well as a preparation method and application of the targeting nanoparticle. Comprising the following steps: (1) combining MP peptide with DSPE-PEG2000-MAL through a maleimide-mercaptan reaction, dialyzing the obtained conjugate, washing, and freeze-drying, so as to obtain a DSPE-PEG2000-MP conjugate; and (2) mixing the conjugate, lipid, a pharmaceutical preparation and a solvent, removing the solvent to obtain a lipid film, hydrating the lipid film, and performing membrane extrusion to obtain the nanoparticles. The invention not only provides a novel efficient and safe ARDS treatment scheme, but also provides an innovative mode for the design of a nano-drug delivery system based on cell mediation. And due to the dual targeting capability, excellent anti-inflammatory efficiency and good biocompatibility, the nano-material has a wide prospect in clinical application.

The application provides an online sales click rate estimation method for agricultural products. The method of the application comprises the following steps: obtaining agricultural product sales related data, including product attributes, user behaviors, context information, etc., and cleaning and preprocessing the data; using a GBDT model to process the features, which can flexibly process various types of data without one-hot encoding processing of discrete features, introducing an Input-aware strategy to refine the weight vector and embedding vector of each feature for different instances, adaptively learning the influence of each input vector on feature representation, and adding a fully connected network to capture high-order features in a nonlinear manner, so that the method has better expression and generalization ability for complex structure data features.

The invention discloses an injectable hydrogel pharmaceutical composition for an articular cavity as well as a preparation method and application of the injectable hydrogel pharmaceutical composition. The hydrogel pharmaceutical composition comprises gel particles and a medicine loaded in the gel particles, the gel particles are formed by cross-linking hyaluronic acid or salt thereof and silk fibroin through a cross-linking agent; the medicine comprises an anti-inflammatory drug and / or a growth factor. The preparation method is simple, good in safety and beneficial to industrial production. The uniform porous structure of the hydrogel is beneficial to adhesion and growth of bone cells, meanwhile, the double-load hydrogel loaded with the anti-inflammatory drug and the growth factor has good viscoelasticity and injectability and has the effects of synergistically resisting inflammation and promoting cartilage repair, and bone joint related diseases are treated in a bone joint intracavity injection mode; the drug residence time can be prolonged, and the bioavailability is improved.

The present application relates to the field of traditional Chinese medicinal materials, in particular to a composition for resisting PRRSv and improving the reproductive performance of sows and application thereof. The present application formulates a composition composed of 10 plant extracts (Angelica sinensis extract, Atractylodes macrocephala Koidz extract, Glycyrrhiza uralensis Fisch extract, Eucommia ulmoides Oliver extract, Eucommia ulmoides leaf extract, Populus davidiana Dode extract, Perilla frutescens leaf extract, Gynostemma pentaphyllum Makino extract, Leonurus japonicus extract and Astragalus membranaceus Bge extract) for sows in late pregnancy, and collects sow blood and placenta samples at the time of delivery, studies the influence of the composition on sow reproduction, and explores the mechanism of action. The experimental results show that the composition can improve the reproductive performance of sows by increasing the total number of piglets and other indicators; meanwhile, the composition can effectively inhibit the replication of PRRSv.

This invention relates to the field of cosmetics, specifically to a composition containing copper peptides for repairing photothermal damage and its application. The composition comprises a complex polypeptide, galactosomal yeast fermentation product filtrate, and cyanobacterial extract; wherein the complex polypeptide includes copper peptides, yeast polypeptides, and hexapeptide-11. This invention, by combining specific complex polypeptides with galactosomal yeast fermentation product filtrate and cyanobacterial extract, increases the expression level of heat shock proteins, thereby achieving excellent efficacy in repairing photothermal damage. Furthermore, this composition is mild and non-irritating, and can be widely used in cosmetics.

The application relates to the field of biotechnology, in particular to recombinant Pichia pastoris expressing recombinant glucoside hydrolase, a preparation method, a preparation and application thereof, further relates to the recombinant glucoside hydrolase, a preparation method of the recombinant glucoside hydrolase, a preparation of the recombinant glucoside hydrolase, a preparation method of the preparation of the recombinant glucoside hydrolase, a product for constructing the recombinant Pichia pastoris and a method for synthesizing ginsenoside CK. The recombinant Pichia pastoris expresses the recombinant glucoside hydrolase, and MicC sRNA and Hfq protein of a target gene. The recombinant Pichia pastoris has the advantages of high expression of the recombinant glucoside hydrolase, high safety, high heat resistance, strong specificity and high transformation efficiency.

The invention provides a silk fibroin composition for injection and a preparation method thereof, and belongs to the technical field of medical cosmetology. The silk fibroin composition is prepared from the following components in percentage by mass: 0.1 to 0.7 weight percent of hyaluronic acid or salt thereof, 0.1 to 10 weight percent of silk fibroin and the balance of phosphate buffer solution. The silk fibroin composition is used for water-light injection, can promote collagen production and inhibit collagen fibrosis, does not have adverse reactions such as skin allergy, hardening and the like, and has the effects of quickly improving the dry state of skin, fading fine wrinkles or wrinkles, replenishing water, preserving moisture and tightening the skin.

The invention discloses a method for cultivating high-starch potatoes through grafting, and relates to the technical field of potato cultivation. The method comprises the following specific steps: (1) cultivating a high-starch potato stock and a common potato scion; (2) grafting the rootstock and the scion; (3) grafted plant treatment; (4) domestication treatment: carrying out stress treatment on the grafted seedlings for 10-15 days, then transferring the grafted seedlings outdoors for culture, and harvesting tubers; and (5) screening of high-starch germplasm: selecting tubers with high starch content for planting, repeatedly domesticating for 2-3 generations, and harvesting tubers to obtain the high-starch potato germplasm. According to the method, the high-starch potato seedlings and the common potato seedlings are grafted, aeroponic culture management is performed after grafting, domestication treatment is performed, the starch content of the grafted potatoes can be increased, and after multi-generation domestication, the high-starch potato germplasm is obtained through breeding. According to the method, the variety breeding process is shortened, and the problem that the high-starch potato germplasm is not created by adopting a grafting method at present is solved.

The application relates to a high-efficiency Pichia pastoris engineering bacterium for expressing I+III type recombinant collagen and a fermentation and purification method thereof, and belongs to the technical field of genetic engineering, microbial fermentation and protein separation and purification. The high-efficiency Pichia pastoris engineering bacterium for expressing I+III type recombinant collagen is a multi-copy Pichia pastoris engineering strain, the Pichia pastoris engineering bacterium contains multiple 2-copy plasmid vectors and / or multiple single-copy plasmid vectors, the plasmid vector contains an I+III type recombinant collagen fusion gene, and the I+III type recombinant collagen fusion gene sequence is shown in SEQ ID NO: 1. The expression amount is high, the stable amplification is easy, and the I+III type recombinant collagen with high purity and high recovery rate can be obtained.

The invention discloses a small-diameter tube welding defect detection method based on space guide feature selection. A used model comprises a backbone network, a pixel decoder and a query decoder. A multi-scale feature map is extracted from an input image through a backbone network, the multi-scale feature map is fused from top to bottom and from bottom to top in a pixel decoder, and a large-scale pixel decoding feature map, a secondary large-scale pixel decoding feature map, a secondary small-scale pixel decoding feature map and a small-scale pixel decoding feature map are obtained; the object query vector interacts with the small-scale pixel decoding feature map, the secondary small-scale pixel decoding feature map and the secondary large-scale pixel decoding feature map in sequence in a query decoder to obtain a prototype query vector; and interacting the prototype query vector with the foreground-enhanced large-scale pixel decoding feature map to obtain a welding defect detection result. Aiming at the characteristics of non-uniform gray distribution, smooth edge and the like of an X-ray small-diameter tube welding image, the backbone network extracts features from multiple angles, and the detection precision is improved.

This invention relates to the field of genetic breeding technology, and specifically discloses... TaGS2‑B The application of genes in regulating wheat yield traits, the aforementioned TaGS2‑B The cDNA sequence of the gene is shown in SEQ ID NO.4, and the amino acid sequence of the protein it encodes is shown in SEQ ID NO.13. This invention improves... TaGS2‑B Gene expression levels increased the number of grains per wheat spike, the number of wheat spikes, and the grain yield per wheat plant.

The present application relates to a kind of mediated zymolytic enzyme of brown algae and application of double transcription unit recombinant bacteria of oxyprotein and brown algae, belong to genetic engineering technical field, the recombinant engineering bacteria is composed of two transcription units, one transcription unit is GAP-alpha-Vgb-AOX1TT, another transcription unit is AOX-alpha-102C300C-AOX1TT, two transcription units are expressed in tandem in pPICZ alphaA plasmid.The present application also provides the preparation method and application of the recombinant bacteria.The recombinant bacteria of the present application can improve the secretion expression efficiency of brown algae zymolytic enzyme, especially can be expressed in low-oxygen environment.

The application belongs to the field of genetic engineering, and particularly relates to a polynucleotide construct capable of expressing LMNA and application thereof in preventing and treating DCM. The synthetic intron in the application comprises: I) a nucleotide sequence as shown in SEQ ID No. 8, II) a nucleotide sequence having at least one mutation in the 1-8th position of the sequence shown in SEQ ID No. 8, or a nucleotide sequence having at least 90% homology with the sequence shown in I) or II). By using the synthetic intron in the application, two proteins can be simultaneously expressed through alternative splicing, and the ratio of the two proteins or the expression of a single protein can be regulated through mutation of the splicing signal. Compared with the existing gene therapy method for dilated cardiomyopathy caused by LMNA mutation, the expression vector for simultaneously expressing Lamin A and Lamin C proteins in the application has stronger rescue effect.

The present invention relates to the technical field of traditional Chinese medicine, and provides an application of a traditional Chinese medicine composition in the preparation of treating postmenopausal osteoporosis. The traditional Chinese medicine composition is prepared from the following components in parts by weight: 25-35 parts of Glossy Privet Fruit, 10-20 parts of Morinda officinalis How, 10-20 parts of Drynaria fortunei (Kunze) J. Sm., and 5-15 parts of Alisma orientale (Sam.) Juz. Among them, Glossy Privet Fruit is the monarch drug, with a mild nature, being good at nourishing the liver and kidney, and replenishing yin and generating essence; Morinda officinalis How and Drynaria fortunei (Kunze) J. Sm. together serve as the ministerial drugs, and Alisma orientale (Sam.) Juz. serves as the assistant and guiding drug. The whole formula can achieve the effects of tonifying both yin and yang, tonifying without stagnation, and dispelling pathogenic factors and strengthening healthy qi.

The application discloses a bovine type I alpha interferon-ferritin fusion protein, a mutant thereof, and a preparation method and application thereof. The bovine type I alpha interferon is fused with a ferritin subunit, the self-assembly characteristics of the ferritin are utilized, the interferon molecules are highly repeated and orderly displayed on the surface of the ferritin nanocage, and the expression level, structural stability and antiviral activity of the interferon are significantly improved. The fusion protein is further subjected to rational design mutation at a unit point or multiple points, and a mutant with significantly improved antiviral activity and stability is obtained. The fusion protein or the mutant thereof is expressed by using a silkworm or insect cell eukaryotic expression system, the expression system is safe in operation, simple in procedure, low in cost, and extremely beneficial to large-scale industrial production, and the prepared fusion protein or mutant nanometer particle has application prospects in the preparation of medicines or reagents for preventing or treating bovine viral diseases.

The invention relates to a method for improving the secretory expression level of expansin in bacillus subtilis, and aims to provide a method capable of promoting the bacillus subtilis to secrete expansin (microorganism-derived expansin BsEXLX1 and plant-derived expansin LeEXP2). The method comprises the following steps: firstly, screening a promoter capable of efficiently starting expansin to construct an engineering strain, and then performing overexpression of a secretory cofactor in the strain to improve the limitation of target protein in the aspects of secretory transport, folding maturation, stability and the like, thereby improving the extracellular secretion level of the two expansin. Amplification verification is carried out through a 5L fermentation tank, the secretion level of expansin is further improved, the expression quantity of BsEXLX1 protein is improved to 1.3 g / L, and the secretion quantity of LeEXP2 is improved to 43.7 mg / L. And a process basis and industrialization potential are provided for subsequent large-scale fermentation production and application development.